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<?xml version='1.0' encoding='ISO-8859-1'?>
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<!DOCTYPE refentry PUBLIC "-//OASIS//DTD DocBook XML V4.4//EN"
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"/usr/share/xml/docbook/schema/dtd/4.4/docbookx.dtd" [
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<!ENTITY dhfirstname "<firstname>Charles</firstname>">
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<!ENTITY dhsurname "<surname>Plessy</surname>">
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<!ENTITY dhdate "<date>avril 29, 2006</date>">
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<!ENTITY dhsection "<manvolnum>1</manvolnum>">
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<!ENTITY dhemail "<email>charles-debian-nospam@plessy.org</email>">
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<!ENTITY dhusername "Charles Plessy">
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<!ENTITY dhucpackage "<refentrytitle>KALIGN</refentrytitle>">
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<!ENTITY dhpackage "kalign">
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<!ENTITY debian "<productname>Debian</productname>">
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<!ENTITY gnu "<acronym>GNU</acronym>">
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<!ENTITY gpl "&gnu; <acronym>GPL</acronym>">
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<!-- Copyright 2006 Charles Plessy. You can use, copy, and redistribute this manpage under the GNU GPL. -->
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<holder>&dhusername;</holder>
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<refname>&dhpackage;</refname>
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<refpurpose>performs multiple alignment of biological sequences.</refpurpose>
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<command>&dhpackage;</command>
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<arg choice="opt">Options</arg>
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<replaceable>infile.fasta</replaceable>
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<replaceable>outfile.fasta</replaceable>
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<command>&dhpackage;</command>
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<arg choice="opt">Options</arg>
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<replaceable>infile.fasta</replaceable>
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<replaceable>outfile.fasta</replaceable>
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<command>&dhpackage;</command>
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<arg choice="opt">Options</arg>
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<arg choice="opt"> <
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<replaceable>infile.fasta</replaceable>
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<arg choice="opt"> >
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<replaceable>outfile.fasta</replaceable>
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<title>DESCRIPTION</title>
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<para><command>Kalign</command> is a command line tool to perform multiple alignment of biological sequences. It employs the Wu-Manber string-matching algorithm, to improve both the accuracy and speed of the alignment. It uses global, progressive alignment approach, enriched by employing an approximate string-matching algorithm to calculate sequence distances and by incorporating local matches into the otherwise global alignment. In comparisons made by its authors, Kalign was about 10 times faster than ClustalW and, depending on the alignment size, up to 50 times faster than popular iterative methods.</para>
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<title>OPTIONS</title>
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<term><option>-i</option>
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<option>-input</option>
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<para>Name of the input file in FASTA format.</para>
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<term><option>-o</option>
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<option>-output</option>
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<para>Name of the output file (in FASTA format).</para>
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<option>-gpo</option>
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<option>-gap_open</option>
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<parameter>x</parameter>
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<para>Gap open penalty (default 6.0).</para>
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<term><option>-gpe</option>
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<option>-gap_ext</option>
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<parameter>x</parameter>
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<para>Gap extension penalty (default 0.9).</para>
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<term><option>-p</option>
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<option>-points</option>
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<para>Wu-Manber algorithm used in both distance calculation and dynamic programming.</para>
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<term><option>-w</option>
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<para>Wu-Manber algorithm will not be used at all.</para>
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<term><option>-f</option>
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<option>-fast</option>
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<para>Fast heuristic alignment ( recommended for >500 sequences)</para>
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<term><option>-q</option>
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<para><quote>quiet</quote> - no messages are sent to standard error.</para>
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<para> In default mode Kalign will use the Wu-Manber algorithm for distance calculation only. (recommended for < 500 sequences)</para>
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<title>REFERENCE</title>
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Please cite Timo Lassmann and Erik L.L. Sonnhammer (2005) Kalign - an accurate and fast multiple sequence alignment algorithm. BMC Bioinformatics 6:298
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<title>COPYRIGHTS</title>
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<para>Copyright (C) 2004, 2005, 2006 Timo Lassmann <email>timo.lassmann@cgb.ki.se</email>. Kalign is free software. You can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation.</para>
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<para>This manual page was written by &dhusername; &dhemail; for
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the &debian; system (but may be used by others). Permission is
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granted to copy, distribute and/or modify this document under
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the terms of the &gnu; General Public License, Version 2 any
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later version published by the Free Software Foundation.
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On Debian systems, the complete text of the GNU General Public
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License can be found in /usr/share/common-licenses/GPL.