~ubuntu-branches/ubuntu/maverick/ncbi-tools6/maverick

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<TITLE> FASTA format description </TITLE>
<!-- Changed by: Sergei Shavirin,  2-Apr-1996 -->
<BODY bgcolor="FFFFFF" link="0000FF" vlink="ff0000" text="000000" >  
<h1>FASTA format description</h1>
<HR>
<p>
<dd>A sequence in FASTA format begins with a single-line description, 
followed by lines of sequence data.  The description line is 
distinguished from the sequence data by a greater-than (">") symbol 
in the first column.  It is recommended that all lines of text be 
shorter than 80 characters in length. An example sequence in FASTA 
<BR>format is:

<PRE>

>gi|532319|pir|TVFV2E|TVFV2E envelope protein
ELRLRYCAPAGFALLKCNDADYDGFKTNCSNVSVVHCTNLMNTTVTTGLLLNGSYSENRT
QIWQKHRTSNDSALILLNKHYNLTVTCKRPGNKTVLPVTIMAGLVFHSQKYNLRLRQAWC
HFPSNWKGAWKEVKEEIVNLPKERYRGTNDPKRIFFQRQWGDPETANLWFNCHGEFFYCK
MDWFLNYLNNLTVDADHNECKNTSGTKSGNKRAPGPCVQRTYVACHIRSVIIWLETISKK
TYAPPREGHLECTSTVTGMTVELNYIPKNRTNVTLSPQIESIWAAELDRYKLVEITPIGF
APTEVRRYTGGHERQKRVPFVXXXXXXXXXXXXXXXXXXXXXXVQSQHLLAGILQQQKNL
LAAVEAQQQMLKLTIWGVK

</PRE>

<dd> Sequences are expected to be represented in the standard 
IUB/IUPAC amino acid and nucleic acid codes, with these 
exceptions:  lower-case letters are accepted and are mapped 
into upper-case; a single hyphen or dash can be used to represent 
a gap of indeterminate length; and in amino acid sequences, U and *
are acceptable letters (see below).  Before submitting a request, 
any numerical digits in the query sequence should either be 
removed or replaced by appropriate letter codes (e.g., N for 
unknown nucleic acid residue or X for unknown amino acid residue).
<BR>
The nucleic acid codes supported are:
<PRE>
        A --> adenosine           M --> A C (amino)
        C --> cytidine            S --> G C (strong)
        G --> guanine             W --> A T (weak)
        T --> thymidine           B --> G T C
        U --> uridine             D --> G A T
        R --> G A (purine)        H --> A C T
        Y --> T C (pyrimidine)    V --> G C A
        K --> G T (keto)          N --> A G C T (any)
                                  -  gap of indeterminate length
</PRE>

For those programs that use amino acid query sequences (BLASTP 
and TBLASTN), the accepted amino acid codes are:
<PRE>

    A  alanine                         P  proline
    B  aspartate or asparagine         Q  glutamine
    C  cystine                         R  arginine
    D  aspartate                       S  serine
    E  glutamate                       T  threonine
    F  phenylalanine                   U  selenocysteine
    G  glycine                         V  valine
    H  histidine                       W  tryptophan
    I  isoleucine                      Y  tyrosine
    K  lysine                          Z  glutamate or glutamine
    L  leucine                         X  any
    M  methionine                      *  translation stop
    N  asparagine                      -  gap of indeterminate length
</PRE>
<HR>