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|
Seq-entry ::=
set {
class pub-set ,
descr {
pub {
pub {
medline {
uid 91288538 ,
em
str "1991 Jul 1" ,
cit {
title {
name "A phorbol ester/diacylglycerol-binding protein encoded
by the unc-13 gene of Caenorhabditis elegans." } ,
authors {
names
str {
"Maruyama IN" ,
"Brenner S" } } ,
from
journal {
title {
ml-jta "Proc. Natl. Acad. Sci. U.S.A." } ,
imp {
date
str "1991 Jul 1" ,
volume "88" ,
issue "13" ,
pages "5729-33" } } } ,
abstract "Mutations in the unc-13 gene cause diverse defects in
the nervous system of the nematode Caenorhabditis elegans. Molecular cloning
of the gene and sequencing of the cDNA revealed that the product encodes a
protein, 1734 amino acids in length, with a central domain with sequence
similarity to the regulatory region of protein kinase C. The domain was
expressed in Escherichia coli and shown to bind specifically to a phorbol
ester in the presence of calcium; diacylglycerol inhibited the binding in a
competitive manner. These findings confirm that the unc-13 gene product has
binding sites similar to those of protein kinase C and may be a component of
an alternative transduction pathway of the diacylglycerol signal to a
different effector function in the nervous system." ,
mesh {
{
term "Amino Acid Sequence" } ,
{
term "Animal" } ,
{
term "Base Sequence" } ,
{
term "Caenorhabditis" } ,
{
term "Chromosome Mapping" } ,
{
term "DNA" } ,
{
term "Genes, Structural" } ,
{
term "Molecular Sequence Data" } ,
{
term "Phorbol 12,13-Dibutyrate" } ,
{
term "Protein Kinase C" } ,
{
term "Receptors, Drug" } ,
{
term "Recombinant Proteins" } ,
{
term "Restriction Mapping" } } ,
substance {
{
type ec ,
cit "2.7.1.-" ,
name "Protein Kinase C" } ,
{
type cas ,
cit "37558-16-0" ,
name "Phorbol 12,13-Dibutyrate" } ,
{
type cas ,
cit "9007-49-2" ,
name "DNA" } } ,
xref {
{
type genbank ,
cit "M62830" } } } } } } ,
seq-set {
set {
class nuc-prot ,
descr {
title "[mRNA] unc-13=phorbol ester/diacylglycerol-binding protein
[Caenorhabditis elegans]; and translated products" ,
org {
taxname "Caenorhabditis elegans" } } ,
seq-set {
seq {
id {
gibbmt 131777 ,
gibbsq 39237 } ,
descr {
mol-type mRNA ,
title "[mRNA] unc-13=phorbol ester/diacylglycerol-binding
protein [Caenorhabditis elegans]" ,
pub {
pub {
muid 91288538 } ,
name "unc-13" ,
fig "fig 3" ,
num
cont {
} } } ,
inst {
repr raw ,
mol rna ,
length 5376 ,
topology linear ,
seq-data
iupacna "GAATTCATTTTTGAAACAAATCGACCTGACGACGGAATGGTACTCGAATTA
TGGGCCAAAGGGTTTTATGGGACAAATTAATAGGTGTTCATTATATGCCACTTTCGGAGATTAGATACAGCAATGCAG
CAGGTTCGGGTCAATGGCTTCAAATGGATCATGAACTTGAAACAAGAAATGGTCAAACTGTAGGAACACGTGGTCCAA
CAGGTCATAACTTGTTGACTGATGTTCGATTTGAGCTTCCTTTTGATGTACAAGGCCACGACGAAGATGTTCAGTCCA
GATTGCTCGCGTTGAACGGACTCATCGAACACGATCAGCTGGGCCCCAACAACCATCACAGGGCGCCGTTTAACCATT
CGGGTTTGAGTGAAGACAGTGATTATACATCCGACGTGTCCGTTCCAGTCAACCATCATCAATTACATCCAAACAGTT
CGGCACATCAATACGAATCACATCTACATCCACATCGAACACGTCAATTGTTACACACTCGTGAAGGCGCAGCGAGTT
ATGAGGACGAAGAAGACGCCTATCACGCTAGACATCAACCCGAATCCGACGACTATAACCACCAAGACACCTACGATC
AACATTCGTCGTATTATAACGACGAATACGCGCCAAGTGGTAGTAGCAGTCAATATCAACGGCAAGGATATCAAGATC
AAGATCAACAACATCAGAATATATACGAGGACACGGTGACTCCGGTTCGAGAATTTGGCGAGTCGGCCGTTCCGCCGG
CTGCTTCATCGTCTAGACGTCAATTCGATCAGGTTTACGGGTACGCATCTAGCAGTGAAGAACGATATGACACACCGA
TGTCAAGTGGTCGACTGCCAAGAGATGAACCAATTCTTGAGCACAGTGAACCAGAATACGTGTACGATCAGAATGGTT
ATCCCGAAGAAGACAACTATGGAATTAATCCAACATATTCGGAAGATCATTTTGAAGGTCAAACAAATGACTATTCTA
CGACACATCAAGAACCTAATGATTTTAGGAATGATTATAATTCTAGTTATCAAAGAGAATATTGGAATGAGAGTGAGC
CGCTTTCTTATAATAGTAGGCCTCCAAACGGACATATTAGAACAGGTGCAAACACTTGGCGCGAACCATCTACGTCAT
CTCGCCCAACATCTTCCCAAGCATGGAATTATCAAGATGACACTCACCAATATGATGAAGTTGATCGTGGATCCCGAG
TATCTTTCACAAGAACACCATCAGTAGATAGAACAGATAGACCAAGTGAAAGTGGTGGAGGATTTTATGATGAAATGT
CAGAAAGTGGAAGACCAGGGCGTCCAGATTCTCACCATAATTGGAGATATGATTCAATTCAAGAAGAAGATAATGAAA
AAGACAACTGGAAACAACATGTGGAAGGCTACGAAGAAGGACAGGAAGAGAAACAAAAGGACAATCAAAAACCTAATG
ATCATAGTGCAGCTTCACCTCAAGATCACTATCATCGATCAGATTCAACAGCGCAACAAGATTTCGGAAACAATATCG
TTCGTCAGACAATTCAAGAAGAAGAGGAAAAAAGGAATTATCAGGAACTTTGGCATAATGCTTACAAGAGAGTTTGTG
CTGATCTCGGAATTAAGAGTACTGTGCTTGACGGGAACGGGAGTTCGGCTGCCAACGCCTTCTACAAATCAATCGATG
CAGCTCCAAACATGAATGTGGCCCGAACGAAGACAAGTATTCCACTTGTTTCCGAACTTGTTCTAAAGACGATGGCCA
CAAAACGTGCGCAAGCAGGTCTGGCGAATGCGGCAAGAACGACGTTCTCAGATACAGAATTAAAAACTCATGTCTACA
AGAAAACTCTACAAGCATTGATCTACCCGATCAGTGCAACAACTCCTCACAACTTCGCAACTACAACATTCCAAACAC
CCACGTTTTGTTACGAATGCGAAGGATTATTGTGGGGATTGGCTAGACAAGGATTGCGATGTACTCAGTGTCAAGTGA
AAGTTCACGATAAATGTCGTGAATTGCTCAGCGCTGATTGTCTTCAAAGAGCAGCCGAGAAGTCGACGAAACATGGAG
AAGCTGATAGAACACAGTCACTTGTCAATGTTATCAGAGATCGAATGAAGATTCAGGAACAGAATAAGCCTGAAGTGT
TTCAGATGATTAGAACAGTTTTTGATGTGGATGAGAATATTCAAAAAGAGACTTTGAAAACTGTAAAGGCGTCTATTT
TGGAAGGAAGTAGCAAATGGAGTGCAAAGATTACATTGACAGTGCTTTGTGCGCAGGGACTTATTGCCAAGGACAAAA
CCGGAAAAAGTGATCCATATGTGACAGCTCAAGTTGGAAAGACCAAACGAAGGACACGAACCATACACCAAGAGCTCA
ATCCAGTTTGGAATGAGAAATTTCATTTTGAATGCCACAACTCCACGGACAGAATTAAAGTTCGAGTTTGGGACGAGG
ATAATGATTTGAAATCCAAGTTAAGGCAAAAATTGACTCGTGAATCTGATGATTTCTTGGGACAAACAGTAATTGAAG
TTCGAACTTTATCTGGTGAAATGGATGTCTGGTATAATCTTGAAAAGAGAACTGATAAATCTGCTGTATCCGGAGCAA
TTCGATTGCATATCAATGTTGAAATCAAGGGAGAAGAGAAACTTGCACCGTATCATGTTCAGTATACATGTCTTCATG
AACATCTTTTTGCTGCTCATTGTGTAGATGAAGAAGTTAAACTTCCAAAAGTTCGAGGCGAAGATAGTTGGAAAGTTT
GCTTCCAGGAAACTGGTCAAGAGATTGCTGAAGAATTTGCGATGCGGTATGGAATTGAAAGTATTTACCAGGCCATGA
CTCATTTCGCATGCCTATGTACTAGATACATGTGCGCAGGAGTACCTGCAGTCTTGTCAACACTTTTGGCCAATATAA
ATGCGTACTATGCTCATACAACTGCCACGTCAGCAGTCTCTGCTCCTGATAGATTTGCTGCATCCAACTTCGGAAAAG
AACGATTTGTGAAACTTCTTGATCAACTTCACAACAGTTTGAGAATTGACTTGTCAGCATATAGGAATCATTTTCCCT
CTTCGTCGCCTGCCAAACTTCAAGATCTTAAATCAACCGTTGATCTTCTTACTTCAATTACATTCTTTCGCATGAAAG
TTCTCGAGTTGGCGAGCCCACCGCGAGCATCAACAGTTGTTCGCGAATGCGCAAAAGCCTGCATGCAGCAGACCTATC
AACTGATGTTTGAATCATGCGCTGAACAATTCCCAATTTTGGACACTTCAGTTCAATTCTGGTATGAATTCATCGACT
ATATAATGCGAGTAATTGAAGAAGATCAAAAAAACTATACACCAGCATTGAATCAATTCCCTCAAGAACTGAATGTTG
GGAATTTATCCGCTGAAACACTTTGGAGTATGTACAAGAATGATTTGAAAATGGCATTAGAAGAACATGCACAAAAGA
AACGATGCAAGACGCCTGAATATATGAATTTATATTTCAAGGTTAAAGGTTTCTATTTCAAATATGTCGCTGATCTTT
CAACATACAAATCATCAATTCCCGAGTTTCCTGCATGGTTTATTCCATTCGTCATGGATTGGCTCAATGAAAATGACG
AACATTCGATGGATATTCTCAGAAATGCATACAACTGTAGACAAGCTGACACTTCCCACAAACATCAGAACACACAAA
TTCTCGAACATGTTGTTGATGTGTTCACCCAACTAAACGCTGCATTGAAGCTCCTCAAACAAATGGACTGTCCAAATC
CAGAAGTTGCTGCTGATATGATGAAGAGATTCAGTAAAACATTGAATAAAGTTCTTCTTGCATACGCTGATATGGTTC
AAAAAGATTTTCCGAAATTTGCACATGACGAGAAGCTTGCGTGTATTCTAATGAACAATGTTCAGCAATTACGTGTGC
AACTCGAAAAGATCTACGAAACAATGGGCGGAGCAGAACTTGACGAACATATTGGACAAGTGCTTACAGTCCTTCAAA
AGAAGTTGAATTCTGTTCTAGACAGACTTTCAGCTGAATTTGTGACCACTTTGGAACCCCATATTCATGAACAAACAA
TCAAATTGGGTATGCTCCTCGTTAAAATAAAGGGTCCACAACTTCAAAAGACACAAGTTCAACCGGAAGCCGATGCAG
TTCTAGAACCGTTGATGGATCTTCTTGAAGGATCATTGAGAAGATACGCGGATCAGTGTGAGAAAACGGTTCTGAAGT
ATATTCTCAAAGAACTTTGGAAAATAACAATTGTTAATATGGAAAAGAGAGTTGTTCTACCACCATTATCAGATAAGG
CTCTATTGAAACAACTGCCGAATGCCAAAATTGGAGATGTTACGAAGTTAATGTCAACAAACAATCAAAGTATAAAGG
GAATGAATTCCGTTAAAGATATGATGGATATGGCAAGAGAATCTGAAAAATCTCTAACACCCAGACAATGTACAGTTC
TTGATTGTGCACTTGACGCCATTAAAGACAGTTTCCATGCATCCGGAAAAGGACTTAAGAAATCATTCTTTGAGAAAT
CACCTGAACTCCAATCTCTCAAATATGCACTCAGTTTATATACACAGACTACTGAGCAACTCATCAAAACGTTTATTA
CAAGCCAGCGACAGCAAGATCTCCCAAGTCAAGAGCAACCAGTTGGAGAAGTTAGTGTTCAAGTTGACTTATTCAGTC
ATCCAGGCACTGGGGAACAGAAGGCTACCGTTAAAATCCTTGCCGCAAACGATCTTCGATGGCAAACCTCGTCAGCTT
TCAAACCATTTGTTGAAGTGCATCTTGTCGGACCACATCTTTCAGACAAAAAGAGAAAATGGTCGACAAAGACCAAAG
CAGGCAACTGGGCTCCAAAATTCAACGAAACTTTTCATTTCTTCCTTGGAAATGAAGGAGAACCTGAACATTACGAGC
TTATGTTTCAAGTCAAGGATTACTGTTTTGCACGAGATGATCGAGTTGTTGGAGTTGGAGTTCTGCAGTTATCATCTG
TCGTTGATCAAGCGGGATCCTGCGCCATGTGGGTTCAACTTGGAACTCGACTGCATATCGACGAGACTGGGCTCATTT
TGCTCAGGATTCTTTCACAGCGTCAAACTGATGAGGTGGCAAAGGACTTTGTACGCCTAAAAACGGAATGCCGATACG
AGACAGAAACAGTAATGGCTGCATCAGCATCAAGTCAAAACATCAATCGAACATAGTGATTCAAGTGCCTTTTTTTGT
TGGATTCAAAGCAATAGAGTTGTTCTTCATTCGATCAATTTTCATCTCCTCTACGAATACCTGTACATTTTGTCGTTT
TTTTTTTCTTTCCTCGAATTC" } ,
annot {
{
data
ftable {
{
id
gibb 131780 ,
data
gene {
locus "unc-13" ,
desc "phorbol ester/diacylglycerol-binding protein" } ,
location
packed-int {
{
from 0 ,
to 5375 ,
strand plus ,
id
gibbsq 39237 } } } } } } } ,
seq {
id {
gibbmt 131799 ,
gibbsq 39239 } ,
descr {
mol-type peptide ,
method both ,
title "[Peptide] phorbol ester/diacylglycerol-binding protein
[Caenorhabditis elegans]" ,
pub {
pub {
muid 91288538 } ,
name "unc-13" ,
fig "fig 3" ,
num
cont {
} } } ,
inst {
repr raw ,
mol aa ,
length 1734 ,
topology linear ,
seq-data
iupacaa "MGQRVLWDKLIGVHYMPLSEIRYSNAAGSGQWLQMDHELETRNGQTVGTRG
PTGHNLLTDVRFELPFDVQGHDEDVQSRLLALNGLIEHDQLGPNNHHRAPFNHSGLSEDSDYTSDVSVPVNHHQLHPN
SSAHQYESHLHPHRTRQLLHTREGAASYEDEEDAYHARHQPESDDYNHQDTYDQHSSYYNDEYAPSGSSSQYQRQGYQ
DQDQQHQNIYEDTVTPVREFGESAVPPAASSSRRQFDQVYGYASSSEERYDTPMSSGRLPRDEPILEHSEPEYVYDQN
GYPEEDNYGINPTYSEDHFEGQTNDYSTTHQEPNDFRNDYNSSYQREYWNESEPLSYNSRPPNGHIRTGANTWREPST
SSRPTSSQAWNYQDDTHQYDEVDRGSRVSFTRTPSVDRTDRPSESGGGFYDEMSESGRPGRPDSHHNWRYDSIQEEDN
EKDNWKQHVEGYEEGQEEKQKDNQKPNDHSAASPQDHYHRSDSTAQQDFGNNIVRQTIQEEEEKRNYQELWHNAYKRV
CADLGIKSTVLDGNGSSAANAFYKSIDAAPNMNVARTKTSIPLVSELVLKTMATKRAQAGLANAARTTFSDTELKTHV
YKKTLQALIYPISATTPHNFATTTFQTPTFCYECEGLLWGLARQGLRCTQCQVKVHDKCRELLSADCLQRAAEKSTKH
GEADRTQSLVNVIRDRMKIQEQNKPEVFQMIRTVFDVDENIQKETLKTVKASILEGSSKWSAKITLTVLCAQGLIAKD
KTGKSDPYVTAQVGKTKRRTRTIHQELNPVWNEKFHFECHNSTDRIKVRVWDEDNDLKSKLRQKLTRESDDFLGQTVI
EVRTLSGEMDVWYNLEKRTDKSAVSGAIRLHINVEIKGEEKLAPYHVQYTCLHEHLFAAHCVDEEVKLPKVRGEDSWK
VCFQETGQEIAEEFAMRYGIESIYQAMTHFACLCTRYMCAGVPAVLSTLLANINAYYAHTTATSAVSAPDRFAASNFG
KERFVKLLDQLHNSLRIDLSAYRNHFPSSSPAKLQDLKSTVDLLTSITFFRMKVLELASPPRASTVVRECAKACMQQT
YQLMFESCAEQFPILDTSVQFWYEFIDYIMRVIEEDQKNYTPALNQFPQELNVGNLSAETLWSMYKNDLKMALEEHAQ
KKRCKTPEYMNLYFKVKGFYFKYVADLSTYKSSIPEFPAWFIPFVMDWLNENDEHSMDILRNAYNCRQADTSHKHQNT
QILEHVVDVFTQLNAALKLLKQMDCPNPEVAADMMKRFSKTLNKVLLAYADMVQKDFPKFAHDEKLACILMNNVQQLR
VQLEKIYETMGGAELDEHIGQVLTVLQKKLNSVLDRLSAEFVTTLEPHIHEQTIKLGMLLVKIKGPQLQKTQVQPEAD
AVLEPLMDLLEGSLRRYADQCEKTVLKYILKELWKITIVNMEKRVVLPPLSDKALLKQLPNAKIGDVTKLMSTNNQSI
KGMNSVKDMMDMARESEKSLTPRQCTVLDCALDAIKDSFHASGKGLKKSFFEKSPELQSLKYALSLYTQTTEQLIKTF
ITSQRQQDLPSQEQPVGEVSVQVDLFSHPGTGEQKATVKILAANDLRWQTSSAFKPFVEVHLVGPHLSDKKRKWSTKT
KAGNWAPKFNETFHFFLGNEGEPEHYELMFQVKDYCFARDDRVVGVGVLQLSSVVDQAGSCAMWVQLGTRLHIDETGL
ILLRILSQRQTDEVAKDFVRLKTECRYETETVMAASASSQNINRT" } ,
annot {
{
data
ftable {
{
id
gibb 131801 ,
data
prot {
desc "phorbol ester/diacylglycerol-binding protein" } ,
location
packed-int {
{
from 0 ,
to 1733 ,
strand plus ,
id
gibbsq 39239 } } } } } } } } ,
annot {
{
data
ftable {
{
id
gibb 131779 ,
data
cdregion {
frame one ,
code {
id 1 } } ,
product
whole
gibbsq 39239 ,
location
packed-int {
{
from 50 ,
to 5251 ,
strand plus ,
id
gibbsq 39237 } } } } } } } } }
|