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## Bioperl Test Harness Script for Modules
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test_begin(-tests => 181,
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-requires_modules => [qw(Clone)]);
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use_ok('Bio::Tools::Run::Phylo::Phast::PhastCons');
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use_ok('Bio::AlignIO');
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use_ok('Bio::TreeIO');
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use_ok('Bio::DB::Taxonomy');
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# setup input files etc
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my $alignfilename = test_input_file("apes.multi_fasta");
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my $treefilename = test_input_file("apes.newick");
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ok (-e $alignfilename, 'Found input alignment file');
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ok (-e $treefilename, 'Found input tree file');
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my $factory = Bio::Tools::Run::Phylo::Phast::PhastCons->new(-verbose => -1,
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'-expected-length' => 3,
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Target_coverage => 0.286,
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isa_ok($factory, 'Bio::Tools::Run::Phylo::Phast::PhastCons');
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ok $factory->can('ignore_missing'), 'has a created method not in args';
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is $factory->expected_length, 3, 'dashed parameter with internal dash was set';
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ok ! $factory->can('Target_coverage'), "wrong-case method wasn't created";
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is $factory->target_coverage, 0.286, 'dashless wrong-case parameter was set';
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is $factory->C, 0.286, 'synonym installed and accessed primary value';
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is $factory->rho, 0.01, 'double-dashed parameter was set';
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# test default factory values
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is ($factory->program_dir, $ENV{'PHASTDIR'}, 'program_dir returned correct default');
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is ($factory->program_name(), 'phastCons', 'Correct exe default name');
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# test the program itself
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test_skip(-requires_executable => $factory,
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# using filename input
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ok my @result1 = $factory->run($alignfilename, $treefilename), 'got results using filename input';
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# using SimpleAlign and Bio::Tree::Tree input
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my $alignio = Bio::AlignIO->new(-file => $alignfilename);
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my $aln = $alignio->next_aln;
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my $treeio = Bio::TreeIO->new(-verbose => -1, -file => $treefilename);
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my $tree = $treeio->next_tree;
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ok my @result2 = $factory->run($aln, $tree), 'got results using object input';
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# using database to generate species tree
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my $tdb = Bio::DB::Taxonomy->new(-source => 'flatfile',
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-directory => test_output_dir(),
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-nodesfile => test_input_file('taxdump','nodes.dmp'),
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-namesfile => test_input_file('taxdump','names.dmp'));
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ok my @result3 = $factory->run($aln, $tdb), 'got results using db input';
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is_deeply \@result1, \@result2, 'results same for file and object input';
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is_deeply \@result1, \@result3, 'results same for file and db input';
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my @apes = qw(human chimpanzee Cross_river_gorilla orangutan common_gibbon crested_gibbon siamang mountain_gorilla Hoolock_gibbon silvery_gibbon);
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is @result1, 20, 'correct number of results';
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foreach my $expected (['apes.1', 10, 14], ['apes.2', 26, 30]) {
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foreach my $i (0..9) {
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my $feat = shift(@result1);
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isa_ok $feat, 'Bio::SeqFeature::Annotated';
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is $feat->seq_id, $apes[$i], 'correct seq_id';
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is $feat->source->value, 'phastCons', 'correct source';
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is ${[$feat->annotation->get_Annotations('Name')]}[0]->value, ${$expected}[0], 'correct feature name';
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is $feat->start, ${$expected}[1], 'correct feature start';
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is $feat->end, ${$expected}[2], 'correct feature end';
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is $feat->score, 6, 'correct feature score';
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is $feat->strand, 1, 'correct feature strand';