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***Evidence Subpage
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#This page is primarily used by large sequencing centers to explain
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annotation prediction methods and its use is optional. The two choices
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of evidence are Experiment or Inference.
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annotation prediction methods and its use is optional. More details
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about these qualifiers can be found in the
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<A HREF="http://www.ncbi.nlm.nih.gov/GenBank/evidence.html">
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genome submission guidelines
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The two choices of evidence are Experiment or Inference.
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#Wet-bench, experimental evidence can be entered as free text in the
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Experiment section. Please be as brief as possible.
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#The Inference section allows for information to be added in cases where
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the feature is annotated based solely on sequence similarity or
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prediction software. There is a controlled format for this qualifier
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which is explained in detail on the
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prediction software. In order to fill in text, you must select one of
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the options from the Category pull-down menu. Different pull-down and
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text boxes will appear depending on the selection you choose from the
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Category menu. If you select one of the 'similar to' categories, you
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must include the name of the database and the corresponding accession
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number of the sequence used as the basis for the annotation. If you
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choose one of the prediction categories, you must include the name and
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version of the prediction program used as the basis for the annotation.
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<A HREF="http://www.insdc.org/feature_table.html#7.4.1">
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. In order to fill in text, you must select one of the options from
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the pull-down menu. The text field must be structured such that the
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evidence basis included is either a reference to a database entry
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(including accession and version number) or an algorithm (including
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version). In the first case, the name of the database must be
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separated from the accession number by a ":". For example, you could
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select "similar to DNA sequence" from the pull-down menu and then enter
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"INSD:AY411252.1" in the text portion. If the annotation is based on a
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prediction algorithm, you would select "ab initio prediction" from the
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pull-down menu and then enter "Genscan:2.0" in the text portion.
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#For example, if your annotation of a coding region was based on
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similarity to the sequence and annotation in GenBank Accession number
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AY411252, you would select "similar to DNA sequence" from the pull-down
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menu and then select "INSD" in the Database pull-down. You would then
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type "AY411252.1" in the Accession text box. If the annotation is
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based on the Genscan prediction algorithm, you would select "ab initio
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prediction" from the pull-down menu, select "Genscan" in the Program
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pull-down and enter 2.0 in the Program Version text box. If the
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database or program used is not listed in the appropriate pull-down
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list, select Other from the list. A new text box will appear where you
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can enter the name of the database or program used. You still must
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include the appropriate accession number or version in the subsequent
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***Identifiers Subpage
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removed
data/sequin.hlp
