1
ACCEPTABLE LEFT PRIMERS
2
0-based # self self qual-
3
# sequence start ln N GC% Tm any end lity
4
0 AACCTGGCTACAGTTCCCTC 71 20 0 55.00 58.255 3.00 0.00 1.745
5
1 AAACCTGGCTACAGTTCCCTC 70 21 0 52.38 59.630 3.00 0.00 1.370
6
2 GAAACCTGGCTACAGTTCCCTC 69 22 0 54.55 61.364 3.00 0.00 3.364
7
3 AGAAACCTGGCTACAGTTCCCTC 68 23 0 52.17 62.164 3.00 0.00 5.164
8
4 AAACCTGGCTACAGTTCCCT 70 20 0 50.00 57.789 3.00 1.00 2.211
9
5 GAAACCTGGCTACAGTTCCCT 69 21 0 52.38 59.630 3.00 1.00 1.370
10
6 AGAAACCTGGCTACAGTTCCCT 68 22 0 50.00 60.528 3.00 2.00 2.528
11
7 GAAACCTGGCTACAGTTCCC 69 20 0 55.00 58.646 3.00 3.00 1.354
12
8 AGAAACCTGGCTACAGTTCCC 68 21 0 52.38 59.630 3.00 3.00 1.370
13
9 CAGAAACCTGGCTACAGTTCCC 67 22 0 54.55 62.245 3.00 3.00 4.245
14
10 CAGAAACCTGGCTACAGTTCC 67 21 0 52.38 58.851 3.00 2.00 2.149
15
11 TCAGAAACCTGGCTACAGTTCC 66 22 0 50.00 60.661 3.00 2.00 2.661
16
12 CTCAGAAACCTGGCTACAGTTCC 65 23 0 52.17 61.514 3.00 2.00 4.514
17
13 TCAGAAACCTGGCTACAGTTC 66 21 0 47.62 57.065 3.00 2.00 3.935
18
14 CTCAGAAACCTGGCTACAGTTC 65 22 0 50.00 58.135 3.00 2.00 3.865
19
15 CCTCAGAAACCTGGCTACAGTTC 64 23 0 52.17 61.514 3.00 2.00 4.514
20
16 CCTCAGAAACCTGGCTACAGTT 64 22 0 50.00 59.810 3.00 2.00 2.190
21
17 CCTCAGAAACCTGGCTACAGT 64 21 0 52.38 58.468 3.00 3.00 2.532
22
18 GCCTCAGAAACCTGGCTACAGT 63 22 0 54.55 62.045 3.00 3.00 4.045
23
19 CCTCAGAAACCTGGCTACAG 64 20 0 55.00 57.547 3.00 3.00 2.453
24
20 GCCTCAGAAACCTGGCTACAG 63 21 0 57.14 61.316 3.00 3.00 2.316
25
21 GCCTCAGAAACCTGGCTACA 63 20 0 55.00 60.397 3.00 1.00 0.397
26
22 GCCTCAGAAACCTGGCTAC 63 19 0 57.89 57.465 3.00 3.00 3.535
27
23 TGCCTCAGAAACCTGGCTAC 62 20 0 55.00 60.397 3.00 3.00 0.397
28
24 GTGCCTCAGAAACCTGGCTAC 61 21 0 57.14 61.195 3.00 3.00 2.195
29
25 TGCCTCAGAAACCTGGCTA 62 19 0 52.63 59.531 3.00 2.00 1.469
30
26 GTGCCTCAGAAACCTGGCTA 61 20 0 55.00 60.397 3.00 2.00 0.397
31
27 TGCCTCAGAAACCTGGCT 62 18 0 55.56 59.499 3.00 3.00 2.501
32
28 GTGCCTCAGAAACCTGGCT 61 19 0 57.89 60.402 3.00 3.00 1.402
33
29 GTGCCTCAGAAACCTGGC 61 18 0 61.11 59.345 3.00 2.00 2.655
34
30 TGTGCCTCAGAAACCTGGC 60 19 0 57.89 62.397 3.00 2.00 3.397
35
31 TGTGCCTCAGAAACCTGG 60 18 0 55.56 58.249 3.00 3.00 3.751
36
32 CTGTGCCTCAGAAACCTGG 59 19 0 57.89 59.399 5.00 3.00 1.601
37
33 CCTGTGCCTCAGAAACCTG 58 19 0 57.89 59.399 5.00 2.00 1.601
38
34 CCCTGTGCCTCAGAAACCT 57 19 0 57.89 60.245 5.00 0.00 1.245
39
35 TCCCTGTGCCTCAGAAACCT 56 20 0 55.00 62.145 5.00 0.00 2.145
40
36 GTCCCTGTGCCTCAGAAACCT 55 21 0 57.14 62.881 5.00 0.00 3.881
41
37 CCCTGTGCCTCAGAAACC 57 18 0 61.11 59.180 5.00 1.00 2.820
42
38 TCCCTGTGCCTCAGAAACC 56 19 0 57.89 61.213 5.00 1.00 2.213
43
39 GTCCCTGTGCCTCAGAAACC 55 20 0 60.00 62.024 5.00 3.00 2.024
44
40 TCCCTGTGCCTCAGAAAC 56 18 0 55.56 57.158 5.00 3.00 4.842
45
41 GTCCCTGTGCCTCAGAAAC 55 19 0 57.89 58.201 5.00 5.00 2.799
46
42 TGTCCCTGTGCCTCAGAAAC 54 20 0 55.00 61.259 5.00 5.00 1.259
47
43 CTGTCCCTGTGCCTCAGAAAC 53 21 0 57.14 62.180 5.00 5.00 3.180
48
44 GTCCCTGTGCCTCAGAAA 55 18 0 55.56 57.158 5.00 3.00 4.842
49
45 TGTCCCTGTGCCTCAGAAA 54 19 0 52.63 60.385 5.00 3.00 1.385
50
46 CTGTCCCTGTGCCTCAGAAA 53 20 0 55.00 61.390 5.00 3.00 1.390
51
47 TGTCCCTGTGCCTCAGAA 54 18 0 55.56 58.812 5.00 1.00 3.188
52
48 CTGTCCCTGTGCCTCAGAA 53 19 0 57.89 59.963 5.00 1.00 1.037
53
49 CTGTCCCTGTGCCTCAGA 53 18 0 61.11 58.348 5.00 3.00 3.652
54
50 CCTGTCCCTGTGCCTCAGA 52 19 0 63.16 62.470 5.00 3.00 3.470
55
51 CCTGTCCCTGTGCCTCAG 52 18 0 66.67 60.405 5.00 5.00 2.405
56
52 TCCTGTCCCTGTGCCTCAG 51 19 0 63.16 62.470 5.00 5.00 3.470
57
53 TCCTGTCCCTGTGCCTCA 51 18 0 61.11 61.468 3.00 3.00 3.468
58
54 TTCCTGTCCCTGTGCCTCA 50 19 0 57.89 62.869 3.00 3.00 3.869
59
55 ATTCCTGTCCCTGTGCCTCA 49 20 0 55.00 62.996 3.00 3.00 2.996
60
56 TATTCCTGTCCCTGTGCCTCA 48 21 0 52.38 62.891 3.00 3.00 3.891
61
57 TTCCTGTCCCTGTGCCTC 50 18 0 61.11 59.749 2.00 1.00 2.251
62
58 ATTCCTGTCCCTGTGCCTC 49 19 0 57.89 60.064 2.00 1.00 1.064
63
59 TATTCCTGTCCCTGTGCCTC 48 20 0 55.00 60.073 2.00 1.00 0.073
64
60 ATATTCCTGTCCCTGTGCCTC 47 21 0 52.38 60.342 4.00 1.00 1.342
65
61 AATATTCCTGTCCCTGTGCCTC 46 22 0 50.00 61.574 6.00 1.00 3.574
66
62 TAATATTCCTGTCCCTGTGCCTC 45 23 0 47.83 61.529 6.00 1.00 4.529
67
63 TTAATATTCCTGTCCCTGTGCCTC 44 24 0 45.83 62.607 6.00 1.00 6.607
68
64 ATTAATATTCCTGTCCCTGTGCCTC 43 25 0 44.00 62.718 6.00 1.00 7.718
69
65 TATTAATATTCCTGTCCCTGTGCCTC 42 26 0 42.31 62.643 8.00 1.00 8.643
70
66 ATATTAATATTCCTGTCCCTGTGCCTC 41 27 0 40.74 62.745 10.00 1.00 9.745
71
67 ATTCCTGTCCCTGTGCCT 49 18 0 55.56 57.991 2.00 0.00 4.009
72
68 TATTCCTGTCCCTGTGCCT 48 19 0 52.63 58.086 2.00 0.00 2.914
73
69 ATATTCCTGTCCCTGTGCCT 47 20 0 50.00 58.473 4.00 0.00 1.527
74
70 AATATTCCTGTCCCTGTGCCT 46 21 0 47.62 59.841 6.00 0.00 1.159
75
71 TAATATTCCTGTCCCTGTGCCT 45 22 0 45.45 59.856 6.00 0.00 2.144
76
72 TTAATATTCCTGTCCCTGTGCCT 44 23 0 43.48 61.044 6.00 0.00 4.044
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73 ATTAATATTCCTGTCCCTGTGCCT 43 24 0 41.67 61.230 6.00 0.00 5.230
78
74 TATTAATATTCCTGTCCCTGTGCCT 42 25 0 40.00 61.200 8.00 0.00 6.200
79
75 ATATTAATATTCCTGTCCCTGTGCCT 41 26 0 38.46 61.365 10.00 0.00 7.365
80
76 AATATTAATATTCCTGTCCCTGTGCCT 40 27 0 37.04 62.311 12.00 0.00 9.311
81
77 ATATTCCTGTCCCTGTGCC 47 19 0 52.63 57.370 4.00 1.00 3.630
82
78 AATATTCCTGTCCCTGTGCC 46 20 0 50.00 58.864 6.00 1.00 1.136
83
79 TAATATTCCTGTCCCTGTGCC 45 21 0 47.62 58.917 6.00 1.00 2.083
84
80 TTAATATTCCTGTCCCTGTGCC 44 22 0 45.45 60.204 6.00 1.00 2.204
85
81 ATTAATATTCCTGTCCCTGTGCC 43 23 0 43.48 60.438 6.00 1.00 3.438
86
82 TATTAATATTCCTGTCCCTGTGCC 42 24 0 41.67 60.432 8.00 1.00 4.432
87
83 ATATTAATATTCCTGTCCCTGTGCC 41 25 0 40.00 60.638 10.00 1.00 5.638
88
84 AATATTAATATTCCTGTCCCTGTGCC 40 26 0 38.46 61.650 12.00 1.00 7.650
89
85 AAATATTAATATTCCTGTCCCTGTGCC 39 27 0 37.04 62.579 12.00 1.00 9.579
90
86 ATTAATATTCCTGTCCCTGTGC 43 22 0 40.91 57.155 6.00 2.00 4.845
91
87 TATTAATATTCCTGTCCCTGTGC 42 23 0 39.13 57.263 8.00 2.00 5.737
92
88 ATATTAATATTCCTGTCCCTGTGC 41 24 0 37.50 57.627 10.00 2.00 6.373
93
89 AATATTAATATTCCTGTCCCTGTGC 40 25 0 36.00 58.823 12.00 2.00 6.177
94
90 AAATATTAATATTCCTGTCCCTGTGC 39 26 0 34.62 59.917 12.00 2.00 6.083
95
91 CAAATATTAATATTCCTGTCCCTGTGC 38 27 0 37.04 62.049 12.00 2.00 9.049
96
92 CAAATATTAATATTCCTGTCCCTGTG 38 26 0 34.62 59.176 12.00 1.00 6.824
97
93 TCAAATATTAATATTCCTGTCCCTGTG 37 27 0 33.33 60.641 12.00 1.00 7.641
98
94 TCAAATATTAATATTCCTGTCCCTGT 37 26 0 30.77 58.425 12.00 0.00 7.575
99
95 CTCAAATATTAATATTCCTGTCCCTGT 36 27 0 33.33 59.222 12.00 0.00 7.778
100
96 TCAAATATTAATATTCCTGTCCCTG 37 25 0 32.00 57.690 12.00 1.00 7.310
101
97 CTCAAATATTAATATTCCTGTCCCTG 36 26 0 34.62 58.540 12.00 1.00 7.460
added
removed
test/primer_list_last/SP01-19.txt.for
