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- Committer: Bazaar Package Importer
- Author(s): Charles Plessy
- Date: 2006-09-28 20:18:54 UTC
- Revision ID: james.westby@ubuntu.com-20060928201854-45pwapz5e3a6d684
Tags: upstream-1.0b
Import upstream version 1.0b
- files added:
- src
- test
- test/primer1_list_last
- test/primer1_list_tmp
- test/primer_global_err
- test/primer_list_last
- test/primer_list_tmp
added
removed
test/primer_start_codon_output
1
PRIMER_PRODUCT_SIZE_RANGE=75-20000
2
PRIMER_MAX_END_STABILITY=10
3
PRIMER_EXPLAIN_FLAG=1
4
PRIMER_MIN_SIZE=19
5
PRIMER_MAX_TM=75
6
PRIMER_NUM_RETURN=1
7
PRIMER_SEQUENCE_ID=test0
8
SEQUENCE=ATTGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTGA
9
PRIMER_START_CODON_POSITION=-3
10
PRIMER_LEFT_EXPLAIN=considered 36, would not amplify any of the ORF 18, low tm 14, ok 4
11
PRIMER_RIGHT_EXPLAIN=considered 33, low tm 1, high end compl 1,high 3' stability 1, ok 30
12
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
13
PRIMER_STOP_CODON_POSITION=75
14
PRIMER_PAIR_PENALTY=69.7977
15
PRIMER_LEFT_PENALTY=68.113745
16
PRIMER_RIGHT_PENALTY=1.683953
17
PRIMER_LEFT_SEQUENCE=ATTGAGAATAGTCTTAGATGTGTCGAA
18
PRIMER_RIGHT_SEQUENCE=ACTGAGCAAGGAAGCTCCG
19
PRIMER_LEFT=0,27
20
PRIMER_RIGHT=75,19
21
PRIMER_LEFT_TM=58.886
22
PRIMER_RIGHT_TM=60.684
23
PRIMER_LEFT_GC_PERCENT=33.333
24
PRIMER_RIGHT_GC_PERCENT=57.895
25
PRIMER_LEFT_SELF_ANY=6.00
26
PRIMER_RIGHT_SELF_ANY=4.00
27
PRIMER_LEFT_SELF_END=2.00
28
PRIMER_RIGHT_SELF_END=2.00
29
PRIMER_LEFT_POSITION_PENALTY=60.000000
30
PRIMER_RIGHT_POSITION_PENALTY=0.000000
31
PRIMER_LEFT_END_STABILITY=8.7000
32
PRIMER_RIGHT_END_STABILITY=9.9000
33
PRIMER_PAIR_COMPL_ANY=3.00
34
PRIMER_PAIR_COMPL_END=2.00
35
PRIMER_PRODUCT_SIZE=76
36
=
37
PRIMER_SEQUENCE_ID=test1
38
SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTAA
39
PRIMER_START_CODON_POSITION=0
40
PRIMER_LEFT_EXPLAIN=considered 36, would not amplify any of the ORF 18, low tm 13, high end compl 1, ok 4
41
PRIMER_RIGHT_EXPLAIN=considered 33, low tm 2,high 3' stability 1, ok 30
42
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
43
PRIMER_STOP_CODON_POSITION=75
44
PRIMER_PAIR_PENALTY=8.2055
45
PRIMER_LEFT_PENALTY=7.037233
46
PRIMER_RIGHT_PENALTY=1.168315
47
PRIMER_LEFT_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCG
48
PRIMER_RIGHT_SEQUENCE=TACTGAGCAAGGAAGCTCCG
49
PRIMER_LEFT=0,25
50
PRIMER_RIGHT=76,20
51
PRIMER_LEFT_TM=57.963
52
PRIMER_RIGHT_TM=60.668
53
PRIMER_LEFT_GC_PERCENT=40.000
54
PRIMER_RIGHT_GC_PERCENT=55.000
55
PRIMER_LEFT_SELF_ANY=6.00
56
PRIMER_RIGHT_SELF_ANY=4.00
57
PRIMER_LEFT_SELF_END=2.00
58
PRIMER_RIGHT_SELF_END=2.00
59
PRIMER_LEFT_POSITION_PENALTY=0.000000
60
PRIMER_RIGHT_POSITION_PENALTY=0.500000
61
PRIMER_LEFT_END_STABILITY=8.4000
62
PRIMER_RIGHT_END_STABILITY=9.9000
63
PRIMER_PAIR_COMPL_ANY=4.00
64
PRIMER_PAIR_COMPL_END=2.00
65
PRIMER_PRODUCT_SIZE=77
66
=
67
PRIMER_SEQUENCE_ID=test2
68
SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTGA
69
PRIMER_START_CODON_POSITION=0
70
PRIMER_LEFT_EXPLAIN=considered 36, would not amplify any of the ORF 18, low tm 13, high end compl 1, ok 4
71
PRIMER_RIGHT_EXPLAIN=considered 33, low tm 1, high end compl 1,high 3' stability 1, ok 30
72
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
73
PRIMER_STOP_CODON_POSITION=75
74
PRIMER_PAIR_PENALTY=8.7212
75
PRIMER_LEFT_PENALTY=7.037233
76
PRIMER_RIGHT_PENALTY=1.683953
77
PRIMER_LEFT_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCG
78
PRIMER_RIGHT_SEQUENCE=ACTGAGCAAGGAAGCTCCG
79
PRIMER_LEFT=0,25
80
PRIMER_RIGHT=75,19
81
PRIMER_LEFT_TM=57.963
82
PRIMER_RIGHT_TM=60.684
83
PRIMER_LEFT_GC_PERCENT=40.000
84
PRIMER_RIGHT_GC_PERCENT=57.895
85
PRIMER_LEFT_SELF_ANY=6.00
86
PRIMER_RIGHT_SELF_ANY=4.00
87
PRIMER_LEFT_SELF_END=2.00
88
PRIMER_RIGHT_SELF_END=2.00
89
PRIMER_LEFT_POSITION_PENALTY=0.000000
90
PRIMER_RIGHT_POSITION_PENALTY=0.000000
91
PRIMER_LEFT_END_STABILITY=8.4000
92
PRIMER_RIGHT_END_STABILITY=9.9000
93
PRIMER_PAIR_COMPL_ANY=4.00
94
PRIMER_PAIR_COMPL_END=2.00
95
PRIMER_PRODUCT_SIZE=76
96
=
97
PRIMER_SEQUENCE_ID=test3
98
SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTGA
99
PRIMER_START_CODON_POSITION=0
100
PRIMER_LEFT_EXPLAIN=considered 36, would not amplify any of the ORF 18, low tm 13, high end compl 1, ok 4
101
PRIMER_RIGHT_EXPLAIN=considered 33, low tm 1, high end compl 1,high 3' stability 1, ok 30
102
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
103
PRIMER_STOP_CODON_POSITION=75
104
PRIMER_PAIR_PENALTY=8.7212
105
PRIMER_LEFT_PENALTY=7.037233
106
PRIMER_RIGHT_PENALTY=1.683953
107
PRIMER_LEFT_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCG
108
PRIMER_RIGHT_SEQUENCE=ACTGAGCAAGGAAGCTCCG
109
PRIMER_LEFT=0,25
110
PRIMER_RIGHT=75,19
111
PRIMER_LEFT_TM=57.963
112
PRIMER_RIGHT_TM=60.684
113
PRIMER_LEFT_GC_PERCENT=40.000
114
PRIMER_RIGHT_GC_PERCENT=57.895
115
PRIMER_LEFT_SELF_ANY=6.00
116
PRIMER_RIGHT_SELF_ANY=4.00
117
PRIMER_LEFT_SELF_END=2.00
118
PRIMER_RIGHT_SELF_END=2.00
119
PRIMER_LEFT_POSITION_PENALTY=0.000000
120
PRIMER_RIGHT_POSITION_PENALTY=0.000000
121
PRIMER_LEFT_END_STABILITY=8.4000
122
PRIMER_RIGHT_END_STABILITY=9.9000
123
PRIMER_PAIR_COMPL_ANY=4.00
124
PRIMER_PAIR_COMPL_END=2.00
125
PRIMER_PRODUCT_SIZE=76
126
=
127
PRIMER_SEQUENCE_ID=test4
128
SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGATA
129
PRIMER_START_CODON_POSITION=0
130
PRIMER_LEFT_EXPLAIN=considered 36, would not amplify any of the ORF 18, low tm 13, high end compl 1, ok 4
131
PRIMER_RIGHT_EXPLAIN=considered 33, low tm 1, high end compl 1,high 3' stability 1, ok 30
132
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
133
PRIMER_STOP_CODON_POSITION=-1
134
PRIMER_PAIR_PENALTY=9.9307
135
PRIMER_LEFT_PENALTY=7.037233
136
PRIMER_RIGHT_PENALTY=2.893427
137
PRIMER_LEFT_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCG
138
PRIMER_RIGHT_SEQUENCE=TATCTGAGCAAGGAAGCTCCG
139
PRIMER_LEFT=0,25
140
PRIMER_RIGHT=77,21
141
PRIMER_LEFT_TM=57.963
142
PRIMER_RIGHT_TM=61.893
143
PRIMER_LEFT_GC_PERCENT=40.000
144
PRIMER_RIGHT_GC_PERCENT=52.381
145
PRIMER_LEFT_SELF_ANY=6.00
146
PRIMER_RIGHT_SELF_ANY=4.00
147
PRIMER_LEFT_SELF_END=2.00
148
PRIMER_RIGHT_SELF_END=2.00
149
PRIMER_LEFT_POSITION_PENALTY=0.000000
150
PRIMER_RIGHT_POSITION_PENALTY=0.000000
151
PRIMER_LEFT_END_STABILITY=8.4000
152
PRIMER_RIGHT_END_STABILITY=9.9000
153
PRIMER_PAIR_COMPL_ANY=5.00
154
PRIMER_PAIR_COMPL_END=2.00
155
PRIMER_PRODUCT_SIZE=78
156
=
157
PRIMER_SEQUENCE_ID=test5
158
SEQUENCE=ATTGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGATA
159
PRIMER_START_CODON_POSITION=0
160
PRIMER_ERROR=No start codon at PRIMER_START_CODON_POSITION
161
=
162
PRIMER_SEQUENCE_ID=test6
163
INCLUDED_REGION=0,299
164
SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGATGAGCGCGCATCTTCAAGTAACCGGTTTTCAAATGAAAGCTTTCACAGCACTGCGCTTCCTCTCAGAACCTTCTGATGCCGTCACAATGCGGGGAGGAAATGTCCTCCTCGACTGCTCCGCGGAGTCCGACCGAGGAGTTCCAGTGATCAAGTGGAAGAAAGATGGCATTCATCTGGCCTTGGGAATGGATGAAAGGAAGCAGCAACTTTCAAATGGGTCTCTGCTGATACAAATGATACTTCATTCCAGA
165
PRIMER_START_CODON_POSITION=298
166
PRIMER_COMMENT=Should fail with "Start codon not contained in INCLUDED_REGION".
167
PRIMER_ERROR=Start codon position not contained in INCLUDED_REGION
168
=
169
PRIMER_SEQUENCE_ID=test7
170
INCLUDED_REGION=0,300
171
SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGATGAGCGCGCATCTTCAAGTAACCGGTTTTCAAATGAAAGCTTTCACAGCACTGCGCTTCCTCTCAGAACCTTCTGATGCCGTCACAATGCGGGGAGGAAATGTCCTCCTCGACTGCTCCGCGGAGTCCGACCGAGGAGTTCCAGTGATCAAGTGGAAGAAAGATGGCATTCATCTGGCCTTGGGAATGGATGAAAGGAAGCAGCAACTTTCAAATGGGTCTCTGCTGATAATGATGATACTTCATTCCATG
172
PRIMER_START_CODON_POSITION=297
173
PRIMER_COMMENT=Should succeed.
174
PRIMER_LEFT_EXPLAIN=considered 1864, would not amplify any of the ORF 1229, low tm 87, high tm 53,high 3' stability 25, ok 470
175
PRIMER_RIGHT_EXPLAIN=considered 1840, low tm 186, high tm 161,high 3' stability 25, ok 1468
176
PRIMER_PAIR_EXPLAIN=considered 1, ok 1
177
PRIMER_STOP_CODON_POSITION=-1
178
PRIMER_PAIR_PENALTY=2163.8303
179
PRIMER_LEFT_PENALTY=2160.043439
180
PRIMER_RIGHT_PENALTY=3.786842
181
PRIMER_LEFT_SEQUENCE=AAAGATGGCATTCATCTGGC
182
PRIMER_RIGHT_SEQUENCE=CATTATCAGCAGAGACCCATTT
183
PRIMER_LEFT=225,20
184
PRIMER_RIGHT=299,22
185
PRIMER_LEFT_TM=60.043
186
PRIMER_RIGHT_TM=58.213
187
PRIMER_LEFT_GC_PERCENT=45.000
188
PRIMER_RIGHT_GC_PERCENT=40.909
189
PRIMER_LEFT_SELF_ANY=6.00
190
PRIMER_RIGHT_SELF_ANY=4.00
191
PRIMER_LEFT_SELF_END=2.00
192
PRIMER_RIGHT_SELF_END=0.00
193
PRIMER_LEFT_POSITION_PENALTY=2160.000000
194
PRIMER_RIGHT_POSITION_PENALTY=0.000000
195
PRIMER_LEFT_END_STABILITY=9.7000
196
PRIMER_RIGHT_END_STABILITY=7.2000
197
PRIMER_PAIR_COMPL_ANY=4.00
198
PRIMER_PAIR_COMPL_END=2.00
199
PRIMER_PRODUCT_SIZE=75
200
=
201
PRIMER_SEQUENCE_ID=test8
202
PRIMER_INSIDE_PENALTY=100.0
203
SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGATA
204
PRIMER_START_CODON_POSITION=0
205
PRIMER_WARNING=Non-default inside penalty or outside penalty has no effect when number of targets is 0
206
PRIMER_ERROR=Cannot accept both PRIMER_START_CODON_POSITION and non-default arguments for PRIMER_INSIDE_PENALTY or PRIMER_OUTSIDE_PENALTY
207
=
208
PRIMER_SEQUENCE_ID=test9
209
PRIMER_INSIDE_PENALTY=-1.0
210
PRIMER_OUTSIDE_PENALTY=100.0
211
SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGATA
212
PRIMER_START_CODON_POSITION=0
213
PRIMER_WARNING=Non-default inside penalty or outside penalty has no effect when number of targets is 0
214
PRIMER_ERROR=Cannot accept both PRIMER_START_CODON_POSITION and non-default arguments for PRIMER_INSIDE_PENALTY or PRIMER_OUTSIDE_PENALTY
215
=
216
PRIMER_PRODUCT_SIZE_RANGE=75-20000
217
PRIMER_OUTSIDE_PENALTY=0.0
218
PRIMER_MAX_END_STABILITY=10
219
PRIMER_EXPLAIN_FLAG=1
220
PRIMER_MIN_SIZE=19
221
PRIMER_MAX_TM=65
222
PRIMER_NUM_RETURN=20
223
PRIMER_SEQUENCE_ID=DCC/X76132
224
SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGCGCGCATCTTCAAGTAACCGGTTTTCAAATTAAAGCTTTCACAGCACTGCGCTTCCTCTCAGAACCTTCTGATGCCGTCACAATGCGGGGAGGAAATGTCCTCCTCGACTGCTCCGCGGAGTCCGACCGAGGAGTTCCAGTGATCAAGTGGAAGAAAGATGGCATTCATCTGGCCTTGGGAATGGATGAAAGGAAGCAGCAACTTTCAAATGGGTCTCTGCTGATACAAAACATACTTCATTCCAGACACCACAAGCCAGATGAGGGACTTTACCAATGTGAGGCATCTTTAGGAGATTCTGGCTCAATTATTAGTCGGACAGCAAAAGTTGCAGTAGCAGGACCACTGAGGTTCCTTTCACAGACAGAATCTGTCACAGCCTTCATGGGAGACACAGTGCTACTCAAGTGTGAAGTCATTGGGGAGCCCATGCCAACAATCCACTGGCAGAAGAACCAACAAGACCTGACTCCAATCCCAGGTGACTCCCGAGTGGTGGTCTTGCCCTCTGGAGCATTGCAGATCAGCCGACTCCAACCGGGGGACATTGGAATTTACCGATGCTCAGCTCGAAATCCAGCCAGCTCAAGAACAGGAAATGAAGCAGAAGTCAGAATTTTATCAGATCCAGGACTGCATAGACAGCTGTATTTTCTGCAAAGACCATCCAATGTAGTAGCCATTGAAGGAAAAGATGCTGTCCTGGAATGTTGTGTTTCTGGCTATCCTCCACCAAGTTTTACCTGGTTACGAGGCGAGGAAGTCATCCAACTCAGGTCTAAAAAGTATTCTTTATTGGGTGGAAGCAACTTGCTTATCTCCAATGTGACAGATGATGACAGTGGAATGTATACCTGTGTTGTCACATATAAAAATGAGAATATTAGTGCCTCTGCAGAGCTCACAGTCTTGGTTCCGCCATGGTTTTTAAATCATCCTTCCAACCTGTATGCCTATGAAAGCATGGATATTGAGTTTGAATGTACAGTCTCTGGAAAGCCTGTGCCCACTGTGAATTGGATGAAGAATGGAGATGTGGTCATTCCTAGTGATTATTTTCAGATAGTGGGAGGAAGCAACTTACGGATACTTGGGGTGGTGAAGTCAGATGAAGGCTTTTATCAATGTGTGGCTGAAAATGAGGCTGGAAATGCCCAGACCAGTGCACAGCTCATTGTCCCTAAGCCTGCAATCCCAAGCTCCAGTGTCCTCCCTTCGGCTCCCAGAGATGTGGTCCCTGTCTTGGTTTCCAGCCGATTTGTCCGTCTCAGCTGGCGCCCACCTGCAGAAGCGAAAGGGAACATTCAAACTTTCACGGTCTTTTTCTCCAGAGAAGGTGACAACAGGGAACGAGCATTGAATACAACACAGCCTGGGTCCCTTCAGCTCACTGTGGGAAACCTGAAGCCAGAAGCCATGTACACCTTTCGAGTTGTGGCTTACAATGAATGGGGACCGGGAGAGAGTTCTCAACCCATCAAGGTGGCCACACAGCCTGAGTTGCAAGTTCCAGGGCCAGTAGAAAACCTGCAAGCTGTATCTACCTCACCTACCTCAATTCTTATTACCTGGGAACCCCCTGCCTATGCAAACGGTCCAGTCCAAGGTTACAGATTGTTCTGCACTGAGGTGTCCACAGGAAAAGAACAGAATATAGAGGTTGATGGACTATCTTATAAACTGGAAGGCCTGAAAAAATTCACCGAATATAGTCTTCGATTCTTAGCTTATAATCGCTATGGTCCGGGCGTCTCTACTGATGATATAACAGTGGTTACACTTTCTGACGTGCCAAGTGCCCCGCCTCAGAACGTCTCCCTGGAAGTGGTCAATTCAAGAAGTATCAAAGTTAGCTGGCTGCCTCCTCCATCAGGAACACAAAATGGATTTATTACCGGCTATAAAATTCGACACAGAAAGACGACCCGCAGGGGTGAGATGGAAACACTGGAGCCAAACAACCTCTGGTACCTATTCACAGGACTGGAGAAAGGAAGTCAGTACAGTTTCCAGGTGTCAGCCATGACAGTCAATGGTACTGGACCACCTTCCAACTGGTATACTGCAGAGACTCCAGAGAATGATCTAGATGAATCTCAAGTTCCTGATCAACCAAGCTCTCTTCATGTGAGGCCCCAGACTAACTGCATCATCATGAGTTGGACTCCTCCCTTGAACCCAAACATCGTGGTGCGAGGTTATATTATCGGTTATGGCGTTGGGAGCCCTTACGCTGAGACAGTGCGTGTGGACAGCAAGCAGCGATATTATTCCATTGAGAGGTTAGAGTCAAGTTCCCATTATGTAATCTCCCTAAAAGCTTTTAACAATGCCGGAGAAGGAGTTCCTCTTTATGAAAGTGCCACCACCAGGTCTATAACCGATCCCACTGACCCAGTTGATTATTATCCTTTGCTTGATGATTTCCCCACCTCGGTCCCAGATCTCTCCACCCCCATGCTCCCACCAGTAGGTGTACAGGCTGTGGCTCTTACCCATGATGCTGTGAGGGTCAGCTGGGCAGACAACTCTGTCCCTAAGAACCAAAAGACGTCTGAGGTGCGACTTTACACCGTCCGGTGGAGAACCAGCTTTTCTGCAAGTGCAAAATACAAGTCAGAAGACACAACATCTCTAAGTTACACAGCAACAGGCCTCAAACCAAACACAATGTATGAATTCTCGGTCATGGTAACAAAAAACAGAAGGTCCAGTACTTGGAGCATGACTGCACATGCCACCACGTATGAAGCAGCCCCCACCTCTGCTCCCAAGGACTTTACAGTCATTACTAGGGAAGGGAAGCCTCGTGCCGTCATTGTGAGTTGGCAGCCTCCCTTGGAAGCCAATGGGAAAATTACTGCTTACATCTTATTTTATACCTTGGACAAGAACATCCCAATTGATGACTGGATTATGGAAACAATCAGTGGTGATAGGCTTACTCATCAAATCATGGATCTCAACCTTGATACTATGTATTACTTTCGAATTCAAGCACGAAATTCAAAAGGAGTGGGGCCACTCTCTGATCCCATCCTCTTCAGGACTCTGAAAGTGGAACACCCTGACAAAATGGCTAATGACCAAGGTCGTCATGGAGATGGAGGTTATTGGCCAGTTGATACTAATTTGATTGATAGAAGCACCCTAAATGAGCCGCCAATTGGACAAATGCACCCCCCGCATGGCAGTGTCACTCCTCAGAAGAACAGCAACCTGCTTGTGATCATTGTGGTCACCGTTGGTGTCATCACAGTGCTGGTAGTGGTCATCGTGGCTGTGATTTGCACCCGACGCTCTTCAGCCCAGCAGAGAAAGAAACGGGCCACCCACAGTGCTGGCAAAAGGAAGGGCAGCCAGAAGGACCTCCGACCCCCTGATCTTTGGATCCATCATGAAGAAATGGAGATGAAAAATATTGAAAAGCCATCTGGCACTGACCCTGCAGGAAGGGACTCTCCCATCCAAAGTTGCCAAGACCTCACACCAGTCAGCCACAGCCAGTCAGAAACCCAACTGGGAAGCAAAAGCACCTCTCATTCAGGTCAAGACACTGAGGAAGCAGGGAGCTCTATGTCCACTCTGGAGAGGTCGCTGGCTGCACGCCGAGCCCCCCGGGCCAAGCTCATGATTCCCATGGATGCCCAGTCCAACAATCCTGCTGTCGTGAGCGCCATCCCGGTGCCAACGCTAGAAAGTGCCCAGTACCCAGGAATCCTCCCGTCTCCCACCTGTGGATATCCCCACCCGCAGTTCACTCTCCGGCCTGTGCCATTCCCAACACTCTCAGTGGACCGAGGTTTCGGAGCAGGAAGAAGTCAGTCAGTGAGTGAAGGACCAACTACCCAACAACCACCTATGCTGCCCCCATCTCAGCCTGAGCATTCTAGCAGCGAGGAGGCACCAAGCAGAACCATCCCCACAGCTTGTGTTCGACCAACTCACCCACTCCGCAGCTTTGCTAATCCTTTGCTACCTCCACCAATGAGTGCAATAGAACCGAAAGTCCCTTACACACCACTTTTGTCTCAGCCAGGGCCCACTCTTCCTAAGACCCATGTGAAAACAGCCTCCCTTGGGTTGGCTGGAAAAGCAAGATCCCCTTTGCTTCCTGTGTCTGTGCCAACAGCCCCTGAAGTGTCTGAGGAGAGCCACAAACCAACAGAGGATTCAGCCAATGTGTATGAACAGGATGATCTGAGTGAACAAATGGCAAGTTTGGAAGGACTCATGAAGCAGCTTAATGCCATCACAGGCTCAGCCTTTTAACATGTATTTCTGAATGGATGAGGTGAATTTTCCGGGAACTTTGCAGCATACCAATTACCCATAAACAGCACACCTGTGTCCAAGAACTCTAACCAGTGTACAGGTCACCCATCAGGACCACTCAGTTAAGGAAGATCCTGAAGCAGTTCAGAAGGAATAAGCATTCCTTCTTTCACAGGCATCAGGAATTGTCAAATGATGATTATGAGTTCCCTAAACAAAAGCAAAGATGCATTTTCACTGCAATGTCAAAGTTTAGCTGCT
225
PRIMER_START_CODON_POSITION=0
226
PRIMER_LEFT_EXPLAIN=considered 39774, would not amplify any of the ORF 27031, GC content failed 49, low tm 2568, high tm 5293, high any compl 25, high end compl 13, long poly-x seq 73,high 3' stability 94, ok 4628
227
PRIMER_RIGHT_EXPLAIN=considered 39257, GC content failed 148, low tm 8235, high tm 15926, high any compl 53, high end compl 11, long poly-x seq 76,high 3' stability 140, ok 14668
228
PRIMER_PAIR_EXPLAIN=considered 58, ok 58
229
PRIMER_STOP_CODON_POSITION=4341
230
PRIMER_PAIR_PENALTY=20.4222
231
PRIMER_LEFT_PENALTY=7.184184
232
PRIMER_RIGHT_PENALTY=13.237993
233
PRIMER_LEFT_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
234
PRIMER_RIGHT_SEQUENCE=TCAGAAATACATGTTAAAAGGCTGA
235
PRIMER_LEFT=0,27
236
PRIMER_RIGHT=4356,25
237
PRIMER_LEFT_TM=60.184
238
PRIMER_RIGHT_TM=59.262
239
PRIMER_LEFT_GC_PERCENT=37.037
240
PRIMER_RIGHT_GC_PERCENT=32.000
241
PRIMER_LEFT_SELF_ANY=6.00
242
PRIMER_RIGHT_SELF_ANY=6.00
243
PRIMER_LEFT_SELF_END=0.00
244
PRIMER_RIGHT_SELF_END=1.00
245
PRIMER_LEFT_POSITION_PENALTY=0.000000
246
PRIMER_RIGHT_POSITION_PENALTY=7.500000
247
PRIMER_LEFT_END_STABILITY=8.8000
248
PRIMER_RIGHT_END_STABILITY=8.2000
249
PRIMER_PAIR_COMPL_ANY=6.00
250
PRIMER_PAIR_COMPL_END=1.00
251
PRIMER_PRODUCT_SIZE=4357
252
PRIMER_PAIR_PENALTY_1=20.5592
253
PRIMER_LEFT_1_PENALTY=7.184184
254
PRIMER_RIGHT_1_PENALTY=13.374972
255
PRIMER_LEFT_1_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
256
PRIMER_RIGHT_1_SEQUENCE=CAGAAATACATGTTAAAAGGCTGA
257
PRIMER_LEFT_1=0,27
258
PRIMER_RIGHT_1=4355,24
259
PRIMER_LEFT_1_TM=60.184
260
PRIMER_RIGHT_1_TM=57.625
261
PRIMER_LEFT_1_GC_PERCENT=37.037
262
PRIMER_RIGHT_1_GC_PERCENT=33.333
263
PRIMER_LEFT_1_SELF_ANY=6.00
264
PRIMER_RIGHT_1_SELF_ANY=6.00
265
PRIMER_LEFT_1_SELF_END=0.00
266
PRIMER_RIGHT_1_SELF_END=1.00
267
PRIMER_LEFT_1_POSITION_PENALTY=0.000000
268
PRIMER_RIGHT_1_POSITION_PENALTY=7.000000
269
PRIMER_LEFT_1_END_STABILITY=8.8000
270
PRIMER_RIGHT_1_END_STABILITY=8.2000
271
PRIMER_PAIR_1_COMPL_ANY=6.00
272
PRIMER_PAIR_1_COMPL_END=1.00
273
PRIMER_PRODUCT_SIZE_1=4356
274
PRIMER_PAIR_PENALTY_2=20.6580
275
PRIMER_LEFT_2_PENALTY=7.184184
276
PRIMER_RIGHT_2_PENALTY=13.473771
277
PRIMER_LEFT_2_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
278
PRIMER_RIGHT_2_SEQUENCE=CAGAAATACATGTTAAAAGGCTGAG
279
PRIMER_LEFT_2=0,27
280
PRIMER_RIGHT_2=4355,25
281
PRIMER_LEFT_2_TM=60.184
282
PRIMER_RIGHT_2_TM=58.526
283
PRIMER_LEFT_2_GC_PERCENT=37.037
284
PRIMER_RIGHT_2_GC_PERCENT=36.000
285
PRIMER_LEFT_2_SELF_ANY=6.00
286
PRIMER_RIGHT_2_SELF_ANY=6.00
287
PRIMER_LEFT_2_SELF_END=0.00
288
PRIMER_RIGHT_2_SELF_END=3.00
289
PRIMER_LEFT_2_POSITION_PENALTY=0.000000
290
PRIMER_RIGHT_2_POSITION_PENALTY=7.000000
291
PRIMER_LEFT_2_END_STABILITY=8.8000
292
PRIMER_RIGHT_2_END_STABILITY=6.7000
293
PRIMER_PAIR_2_COMPL_ANY=6.00
294
PRIMER_PAIR_2_COMPL_END=0.00
295
PRIMER_PRODUCT_SIZE_2=4356
296
PRIMER_PAIR_PENALTY_3=20.7611
297
PRIMER_LEFT_3_PENALTY=7.184184
298
PRIMER_RIGHT_3_PENALTY=13.576946
299
PRIMER_LEFT_3_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
300
PRIMER_RIGHT_3_SEQUENCE=TCAGAAATACATGTTAAAAGGCTGAG
301
PRIMER_LEFT_3=0,27
302
PRIMER_RIGHT_3=4356,26
303
PRIMER_LEFT_3_TM=60.184
304
PRIMER_RIGHT_3_TM=60.077
305
PRIMER_LEFT_3_GC_PERCENT=37.037
306
PRIMER_RIGHT_3_GC_PERCENT=34.615
307
PRIMER_LEFT_3_SELF_ANY=6.00
308
PRIMER_RIGHT_3_SELF_ANY=6.00
309
PRIMER_LEFT_3_SELF_END=0.00
310
PRIMER_RIGHT_3_SELF_END=3.00
311
PRIMER_LEFT_3_POSITION_PENALTY=0.000000
312
PRIMER_RIGHT_3_POSITION_PENALTY=7.500000
313
PRIMER_LEFT_3_END_STABILITY=8.8000
314
PRIMER_RIGHT_3_END_STABILITY=6.7000
315
PRIMER_PAIR_3_COMPL_ANY=6.00
316
PRIMER_PAIR_3_COMPL_END=0.00
317
PRIMER_PRODUCT_SIZE_3=4357
318
PRIMER_PAIR_PENALTY_4=21.0592
319
PRIMER_LEFT_4_PENALTY=7.184184
320
PRIMER_RIGHT_4_PENALTY=13.874972
321
PRIMER_LEFT_4_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
322
PRIMER_RIGHT_4_SEQUENCE=TCAGAAATACATGTTAAAAGGCTG
323
PRIMER_LEFT_4=0,27
324
PRIMER_RIGHT_4=4356,24
325
PRIMER_LEFT_4_TM=60.184
326
PRIMER_RIGHT_4_TM=57.625
327
PRIMER_LEFT_4_GC_PERCENT=37.037
328
PRIMER_RIGHT_4_GC_PERCENT=33.333
329
PRIMER_LEFT_4_SELF_ANY=6.00
330
PRIMER_RIGHT_4_SELF_ANY=6.00
331
PRIMER_LEFT_4_SELF_END=0.00
332
PRIMER_RIGHT_4_SELF_END=1.00
333
PRIMER_LEFT_4_POSITION_PENALTY=0.000000
334
PRIMER_RIGHT_4_POSITION_PENALTY=7.500000
335
PRIMER_LEFT_4_END_STABILITY=8.8000
336
PRIMER_RIGHT_4_END_STABILITY=9.7000
337
PRIMER_PAIR_4_COMPL_ANY=6.00
338
PRIMER_PAIR_4_COMPL_END=1.00
339
PRIMER_PRODUCT_SIZE_4=4357
340
PRIMER_PAIR_PENALTY_5=21.3353
341
PRIMER_LEFT_5_PENALTY=7.184184
342
PRIMER_RIGHT_5_PENALTY=14.151147
343
PRIMER_LEFT_5_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
344
PRIMER_RIGHT_5_SEQUENCE=TTCAGAAATACATGTTAAAAGGCTG
345
PRIMER_LEFT_5=0,27
346
PRIMER_RIGHT_5=4357,25
347
PRIMER_LEFT_5_TM=60.184
348
PRIMER_RIGHT_5_TM=58.849
349
PRIMER_LEFT_5_GC_PERCENT=37.037
350
PRIMER_RIGHT_5_GC_PERCENT=32.000
351
PRIMER_LEFT_5_SELF_ANY=6.00
352
PRIMER_RIGHT_5_SELF_ANY=6.00
353
PRIMER_LEFT_5_SELF_END=0.00
354
PRIMER_RIGHT_5_SELF_END=1.00
355
PRIMER_LEFT_5_POSITION_PENALTY=0.000000
356
PRIMER_RIGHT_5_POSITION_PENALTY=8.000000
357
PRIMER_LEFT_5_END_STABILITY=8.8000
358
PRIMER_RIGHT_5_END_STABILITY=9.7000
359
PRIMER_PAIR_5_COMPL_ANY=6.00
360
PRIMER_PAIR_5_COMPL_END=1.00
361
PRIMER_PRODUCT_SIZE_5=4358
362
PRIMER_PAIR_PENALTY_6=21.5665
363
PRIMER_LEFT_6_PENALTY=7.184184
364
PRIMER_RIGHT_6_PENALTY=14.382282
365
PRIMER_LEFT_6_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
366
PRIMER_RIGHT_6_SEQUENCE=TTCAGAAATACATGTTAAAAGGCTGA
367
PRIMER_LEFT_6=0,27
368
PRIMER_RIGHT_6=4357,26
369
PRIMER_LEFT_6_TM=60.184
370
PRIMER_RIGHT_6_TM=60.382
371
PRIMER_LEFT_6_GC_PERCENT=37.037
372
PRIMER_RIGHT_6_GC_PERCENT=30.769
373
PRIMER_LEFT_6_SELF_ANY=6.00
374
PRIMER_RIGHT_6_SELF_ANY=6.00
375
PRIMER_LEFT_6_SELF_END=0.00
376
PRIMER_RIGHT_6_SELF_END=1.00
377
PRIMER_LEFT_6_POSITION_PENALTY=0.000000
378
PRIMER_RIGHT_6_POSITION_PENALTY=8.000000
379
PRIMER_LEFT_6_END_STABILITY=8.8000
380
PRIMER_RIGHT_6_END_STABILITY=8.2000
381
PRIMER_PAIR_6_COMPL_ANY=6.00
382
PRIMER_PAIR_6_COMPL_END=1.00
383
PRIMER_PRODUCT_SIZE_6=4358
384
PRIMER_PAIR_PENALTY_7=22.0762
385
PRIMER_LEFT_7_PENALTY=8.838215
386
PRIMER_RIGHT_7_PENALTY=13.237993
387
PRIMER_LEFT_7_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG
388
PRIMER_RIGHT_7_SEQUENCE=TCAGAAATACATGTTAAAAGGCTGA
389
PRIMER_LEFT_7=0,26
390
PRIMER_RIGHT_7=4356,25
391
PRIMER_LEFT_7_TM=57.162
392
PRIMER_RIGHT_7_TM=59.262
393
PRIMER_LEFT_7_GC_PERCENT=34.615
394
PRIMER_RIGHT_7_GC_PERCENT=32.000
395
PRIMER_LEFT_7_SELF_ANY=6.00
396
PRIMER_RIGHT_7_SELF_ANY=6.00
397
PRIMER_LEFT_7_SELF_END=0.00
398
PRIMER_RIGHT_7_SELF_END=1.00
399
PRIMER_LEFT_7_POSITION_PENALTY=0.000000
400
PRIMER_RIGHT_7_POSITION_PENALTY=7.500000
401
PRIMER_LEFT_7_END_STABILITY=7.0000
402
PRIMER_RIGHT_7_END_STABILITY=8.2000
403
PRIMER_PAIR_7_COMPL_ANY=6.00
404
PRIMER_PAIR_7_COMPL_END=2.00
405
PRIMER_PRODUCT_SIZE_7=4357
406
PRIMER_PAIR_PENALTY_8=22.2132
407
PRIMER_LEFT_8_PENALTY=8.838215
408
PRIMER_RIGHT_8_PENALTY=13.374972
409
PRIMER_LEFT_8_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG
410
PRIMER_RIGHT_8_SEQUENCE=CAGAAATACATGTTAAAAGGCTGA
411
PRIMER_LEFT_8=0,26
412
PRIMER_RIGHT_8=4355,24
413
PRIMER_LEFT_8_TM=57.162
414
PRIMER_RIGHT_8_TM=57.625
415
PRIMER_LEFT_8_GC_PERCENT=34.615
416
PRIMER_RIGHT_8_GC_PERCENT=33.333
417
PRIMER_LEFT_8_SELF_ANY=6.00
418
PRIMER_RIGHT_8_SELF_ANY=6.00
419
PRIMER_LEFT_8_SELF_END=0.00
420
PRIMER_RIGHT_8_SELF_END=1.00
421
PRIMER_LEFT_8_POSITION_PENALTY=0.000000
422
PRIMER_RIGHT_8_POSITION_PENALTY=7.000000
423
PRIMER_LEFT_8_END_STABILITY=7.0000
424
PRIMER_RIGHT_8_END_STABILITY=8.2000
425
PRIMER_PAIR_8_COMPL_ANY=6.00
426
PRIMER_PAIR_8_COMPL_END=2.00
427
PRIMER_PRODUCT_SIZE_8=4356
428
PRIMER_PAIR_PENALTY_9=22.3120
429
PRIMER_LEFT_9_PENALTY=8.838215
430
PRIMER_RIGHT_9_PENALTY=13.473771
431
PRIMER_LEFT_9_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG
432
PRIMER_RIGHT_9_SEQUENCE=CAGAAATACATGTTAAAAGGCTGAG
433
PRIMER_LEFT_9=0,26
434
PRIMER_RIGHT_9=4355,25
435
PRIMER_LEFT_9_TM=57.162
436
PRIMER_RIGHT_9_TM=58.526
437
PRIMER_LEFT_9_GC_PERCENT=34.615
438
PRIMER_RIGHT_9_GC_PERCENT=36.000
439
PRIMER_LEFT_9_SELF_ANY=6.00
440
PRIMER_RIGHT_9_SELF_ANY=6.00
441
PRIMER_LEFT_9_SELF_END=0.00
442
PRIMER_RIGHT_9_SELF_END=3.00
443
PRIMER_LEFT_9_POSITION_PENALTY=0.000000
444
PRIMER_RIGHT_9_POSITION_PENALTY=7.000000
445
PRIMER_LEFT_9_END_STABILITY=7.0000
446
PRIMER_RIGHT_9_END_STABILITY=6.7000
447
PRIMER_PAIR_9_COMPL_ANY=6.00
448
PRIMER_PAIR_9_COMPL_END=1.00
449
PRIMER_PRODUCT_SIZE_9=4356
450
PRIMER_PAIR_PENALTY_10=22.4152
451
PRIMER_LEFT_10_PENALTY=8.838215
452
PRIMER_RIGHT_10_PENALTY=13.576946
453
PRIMER_LEFT_10_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG
454
PRIMER_RIGHT_10_SEQUENCE=TCAGAAATACATGTTAAAAGGCTGAG
455
PRIMER_LEFT_10=0,26
456
PRIMER_RIGHT_10=4356,26
457
PRIMER_LEFT_10_TM=57.162
458
PRIMER_RIGHT_10_TM=60.077
459
PRIMER_LEFT_10_GC_PERCENT=34.615
460
PRIMER_RIGHT_10_GC_PERCENT=34.615
461
PRIMER_LEFT_10_SELF_ANY=6.00
462
PRIMER_RIGHT_10_SELF_ANY=6.00
463
PRIMER_LEFT_10_SELF_END=0.00
464
PRIMER_RIGHT_10_SELF_END=3.00
465
PRIMER_LEFT_10_POSITION_PENALTY=0.000000
466
PRIMER_RIGHT_10_POSITION_PENALTY=7.500000
467
PRIMER_LEFT_10_END_STABILITY=7.0000
468
PRIMER_RIGHT_10_END_STABILITY=6.7000
469
PRIMER_PAIR_10_COMPL_ANY=6.00
470
PRIMER_PAIR_10_COMPL_END=1.00
471
PRIMER_PRODUCT_SIZE_10=4357
472
PRIMER_PAIR_PENALTY_11=22.5640
473
PRIMER_LEFT_11_PENALTY=7.184184
474
PRIMER_RIGHT_11_PENALTY=15.379819
475
PRIMER_LEFT_11_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
476
PRIMER_RIGHT_11_SEQUENCE=ATTCAGAAATACATGTTAAAAGGCTG
477
PRIMER_LEFT_11=0,27
478
PRIMER_RIGHT_11=4358,26
479
PRIMER_LEFT_11_TM=60.184
480
PRIMER_RIGHT_11_TM=59.120
481
PRIMER_LEFT_11_GC_PERCENT=37.037
482
PRIMER_RIGHT_11_GC_PERCENT=30.769
483
PRIMER_LEFT_11_SELF_ANY=6.00
484
PRIMER_RIGHT_11_SELF_ANY=6.00
485
PRIMER_LEFT_11_SELF_END=0.00
486
PRIMER_RIGHT_11_SELF_END=1.00
487
PRIMER_LEFT_11_POSITION_PENALTY=0.000000
488
PRIMER_RIGHT_11_POSITION_PENALTY=8.500000
489
PRIMER_LEFT_11_END_STABILITY=8.8000
490
PRIMER_RIGHT_11_END_STABILITY=9.7000
491
PRIMER_PAIR_11_COMPL_ANY=6.00
492
PRIMER_PAIR_11_COMPL_END=1.00
493
PRIMER_PRODUCT_SIZE_11=4359
494
PRIMER_PAIR_PENALTY_12=22.7132
495
PRIMER_LEFT_12_PENALTY=8.838215
496
PRIMER_RIGHT_12_PENALTY=13.874972
497
PRIMER_LEFT_12_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG
498
PRIMER_RIGHT_12_SEQUENCE=TCAGAAATACATGTTAAAAGGCTG
499
PRIMER_LEFT_12=0,26
500
PRIMER_RIGHT_12=4356,24
501
PRIMER_LEFT_12_TM=57.162
502
PRIMER_RIGHT_12_TM=57.625
503
PRIMER_LEFT_12_GC_PERCENT=34.615
504
PRIMER_RIGHT_12_GC_PERCENT=33.333
505
PRIMER_LEFT_12_SELF_ANY=6.00
506
PRIMER_RIGHT_12_SELF_ANY=6.00
507
PRIMER_LEFT_12_SELF_END=0.00
508
PRIMER_RIGHT_12_SELF_END=1.00
509
PRIMER_LEFT_12_POSITION_PENALTY=0.000000
510
PRIMER_RIGHT_12_POSITION_PENALTY=7.500000
511
PRIMER_LEFT_12_END_STABILITY=7.0000
512
PRIMER_RIGHT_12_END_STABILITY=9.7000
513
PRIMER_PAIR_12_COMPL_ANY=6.00
514
PRIMER_PAIR_12_COMPL_END=2.00
515
PRIMER_PRODUCT_SIZE_12=4357
516
PRIMER_PAIR_PENALTY_13=22.8956
517
PRIMER_LEFT_13_PENALTY=7.184184
518
PRIMER_RIGHT_13_PENALTY=15.711440
519
PRIMER_LEFT_13_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
520
PRIMER_RIGHT_13_SEQUENCE=CATTCAGAAATACATGTTAAAAGGC
521
PRIMER_LEFT_13=0,27
522
PRIMER_RIGHT_13=4359,25
523
PRIMER_LEFT_13_TM=60.184
524
PRIMER_RIGHT_13_TM=58.289
525
PRIMER_LEFT_13_GC_PERCENT=37.037
526
PRIMER_RIGHT_13_GC_PERCENT=32.000
527
PRIMER_LEFT_13_SELF_ANY=6.00
528
PRIMER_RIGHT_13_SELF_ANY=6.00
529
PRIMER_LEFT_13_SELF_END=0.00
530
PRIMER_RIGHT_13_SELF_END=2.00
531
PRIMER_LEFT_13_POSITION_PENALTY=0.000000
532
PRIMER_RIGHT_13_POSITION_PENALTY=9.000000
533
PRIMER_LEFT_13_END_STABILITY=8.8000
534
PRIMER_RIGHT_13_END_STABILITY=9.7000
535
PRIMER_PAIR_13_COMPL_ANY=6.00
536
PRIMER_PAIR_13_COMPL_END=1.00
537
PRIMER_PRODUCT_SIZE_13=4360
538
PRIMER_PAIR_PENALTY_14=22.9894
539
PRIMER_LEFT_14_PENALTY=8.838215
540
PRIMER_RIGHT_14_PENALTY=14.151147
541
PRIMER_LEFT_14_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG
542
PRIMER_RIGHT_14_SEQUENCE=TTCAGAAATACATGTTAAAAGGCTG
543
PRIMER_LEFT_14=0,26
544
PRIMER_RIGHT_14=4357,25
545
PRIMER_LEFT_14_TM=57.162
546
PRIMER_RIGHT_14_TM=58.849
547
PRIMER_LEFT_14_GC_PERCENT=34.615
548
PRIMER_RIGHT_14_GC_PERCENT=32.000
549
PRIMER_LEFT_14_SELF_ANY=6.00
550
PRIMER_RIGHT_14_SELF_ANY=6.00
551
PRIMER_LEFT_14_SELF_END=0.00
552
PRIMER_RIGHT_14_SELF_END=1.00
553
PRIMER_LEFT_14_POSITION_PENALTY=0.000000
554
PRIMER_RIGHT_14_POSITION_PENALTY=8.000000
555
PRIMER_LEFT_14_END_STABILITY=7.0000
556
PRIMER_RIGHT_14_END_STABILITY=9.7000
557
PRIMER_PAIR_14_COMPL_ANY=6.00
558
PRIMER_PAIR_14_COMPL_END=2.00
559
PRIMER_PRODUCT_SIZE_14=4358
560
PRIMER_PAIR_PENALTY_15=23.0640
561
PRIMER_LEFT_15_PENALTY=7.184184
562
PRIMER_RIGHT_15_PENALTY=15.879819
563
PRIMER_LEFT_15_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
564
PRIMER_RIGHT_15_SEQUENCE=CATTCAGAAATACATGTTAAAAGGCT
565
PRIMER_LEFT_15=0,27
566
PRIMER_RIGHT_15=4359,26
567
PRIMER_LEFT_15_TM=60.184
568
PRIMER_RIGHT_15_TM=59.120
569
PRIMER_LEFT_15_GC_PERCENT=37.037
570
PRIMER_RIGHT_15_GC_PERCENT=30.769
571
PRIMER_LEFT_15_SELF_ANY=6.00
572
PRIMER_RIGHT_15_SELF_ANY=6.00
573
PRIMER_LEFT_15_SELF_END=0.00
574
PRIMER_RIGHT_15_SELF_END=3.00
575
PRIMER_LEFT_15_POSITION_PENALTY=0.000000
576
PRIMER_RIGHT_15_POSITION_PENALTY=9.000000
577
PRIMER_LEFT_15_END_STABILITY=8.8000
578
PRIMER_RIGHT_15_END_STABILITY=9.4000
579
PRIMER_PAIR_15_COMPL_ANY=6.00
580
PRIMER_PAIR_15_COMPL_END=2.00
581
PRIMER_PRODUCT_SIZE_15=4360
582
PRIMER_PAIR_PENALTY_16=23.2205
583
PRIMER_LEFT_16_PENALTY=8.838215
584
PRIMER_RIGHT_16_PENALTY=14.382282
585
PRIMER_LEFT_16_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG
586
PRIMER_RIGHT_16_SEQUENCE=TTCAGAAATACATGTTAAAAGGCTGA
587
PRIMER_LEFT_16=0,26
588
PRIMER_RIGHT_16=4357,26
589
PRIMER_LEFT_16_TM=57.162
590
PRIMER_RIGHT_16_TM=60.382
591
PRIMER_LEFT_16_GC_PERCENT=34.615
592
PRIMER_RIGHT_16_GC_PERCENT=30.769
593
PRIMER_LEFT_16_SELF_ANY=6.00
594
PRIMER_RIGHT_16_SELF_ANY=6.00
595
PRIMER_LEFT_16_SELF_END=0.00
596
PRIMER_RIGHT_16_SELF_END=1.00
597
PRIMER_LEFT_16_POSITION_PENALTY=0.000000
598
PRIMER_RIGHT_16_POSITION_PENALTY=8.000000
599
PRIMER_LEFT_16_END_STABILITY=7.0000
600
PRIMER_RIGHT_16_END_STABILITY=8.2000
601
PRIMER_PAIR_16_COMPL_ANY=6.00
602
PRIMER_PAIR_16_COMPL_END=2.00
603
PRIMER_PRODUCT_SIZE_16=4358
604
PRIMER_PAIR_PENALTY_17=23.2662
605
PRIMER_LEFT_17_PENALTY=7.184184
606
PRIMER_RIGHT_17_PENALTY=16.081991
607
PRIMER_LEFT_17_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
608
PRIMER_RIGHT_17_SEQUENCE=ATTCAGAAATACATGTTAAAAGGCTGA
609
PRIMER_LEFT_17=0,27
610
PRIMER_RIGHT_17=4358,27
611
PRIMER_LEFT_17_TM=60.184
612
PRIMER_RIGHT_17_TM=60.582
613
PRIMER_LEFT_17_GC_PERCENT=37.037
614
PRIMER_RIGHT_17_GC_PERCENT=29.630
615
PRIMER_LEFT_17_SELF_ANY=6.00
616
PRIMER_RIGHT_17_SELF_ANY=6.00
617
PRIMER_LEFT_17_SELF_END=0.00
618
PRIMER_RIGHT_17_SELF_END=1.00
619
PRIMER_LEFT_17_POSITION_PENALTY=0.000000
620
PRIMER_RIGHT_17_POSITION_PENALTY=8.500000
621
PRIMER_LEFT_17_END_STABILITY=8.8000
622
PRIMER_RIGHT_17_END_STABILITY=8.2000
623
PRIMER_PAIR_17_COMPL_ANY=6.00
624
PRIMER_PAIR_17_COMPL_END=1.00
625
PRIMER_PRODUCT_SIZE_17=4359
626
PRIMER_PAIR_PENALTY_18=23.3048
627
PRIMER_LEFT_18_PENALTY=7.184184
628
PRIMER_RIGHT_18_PENALTY=16.120652
629
PRIMER_LEFT_18_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
630
PRIMER_RIGHT_18_SEQUENCE=TTCAGAAATACATGTTAAAAGGCTGAG
631
PRIMER_LEFT_18=0,27
632
PRIMER_RIGHT_18=4357,27
633
PRIMER_LEFT_18_TM=60.184
634
PRIMER_RIGHT_18_TM=61.121
635
PRIMER_LEFT_18_GC_PERCENT=37.037
636
PRIMER_RIGHT_18_GC_PERCENT=33.333
637
PRIMER_LEFT_18_SELF_ANY=6.00
638
PRIMER_RIGHT_18_SELF_ANY=6.00
639
PRIMER_LEFT_18_SELF_END=0.00
640
PRIMER_RIGHT_18_SELF_END=3.00
641
PRIMER_LEFT_18_POSITION_PENALTY=0.000000
642
PRIMER_RIGHT_18_POSITION_PENALTY=8.000000
643
PRIMER_LEFT_18_END_STABILITY=8.8000
644
PRIMER_RIGHT_18_END_STABILITY=6.7000
645
PRIMER_PAIR_18_COMPL_ANY=6.00
646
PRIMER_PAIR_18_COMPL_END=0.00
647
PRIMER_PRODUCT_SIZE_18=4358
648
PRIMER_PAIR_PENALTY_19=23.8715
649
PRIMER_LEFT_19_PENALTY=7.184184
650
PRIMER_RIGHT_19_PENALTY=16.687284
651
PRIMER_LEFT_19_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG
652
PRIMER_RIGHT_19_SEQUENCE=CCATTCAGAAATACATGTTAAAAGGC
653
PRIMER_LEFT_19=0,27
654
PRIMER_RIGHT_19=4360,26
655
PRIMER_LEFT_19_TM=60.184
656
PRIMER_RIGHT_19_TM=61.187
657
PRIMER_LEFT_19_GC_PERCENT=37.037
658
PRIMER_RIGHT_19_GC_PERCENT=34.615
659
PRIMER_LEFT_19_SELF_ANY=6.00
660
PRIMER_RIGHT_19_SELF_ANY=6.00
661
PRIMER_LEFT_19_SELF_END=0.00
662
PRIMER_RIGHT_19_SELF_END=2.00
663
PRIMER_LEFT_19_POSITION_PENALTY=0.000000
664
PRIMER_RIGHT_19_POSITION_PENALTY=9.500000
665
PRIMER_LEFT_19_END_STABILITY=8.8000
666
PRIMER_RIGHT_19_END_STABILITY=9.7000
667
PRIMER_PAIR_19_COMPL_ANY=6.00
668
PRIMER_PAIR_19_COMPL_END=1.00
669
PRIMER_PRODUCT_SIZE_19=4361
670
=
671
PRIMER_SEQUENCE_ID=LEU8/L-SELECTING/X17519
672
SEQUENCE=CTCCCTTTGGGCAAGGACCTGAGACCCTTGTGCTAAGTCAAGAGGCTCAATGGGCTGCAGAAGAACTAGAGAAGGACCAAGCAAAGCCATGATATTTCCATGGAAATGTCAGAGCACCCAGAGGGACTTATGGAACATCTTCAAGTTGTGGGGGTGGACAATGCTCTGTTGTGATTTCCTGGCACATCATGGAACCGACTGCTGGACTTACCATTATTCTGAAAAACCCATGAACTGGCAAAGGGCTAGAAGATTCTGCCGAGACAATTACACAGATTTAGTTGCCATACAAAACAAGGCGGAAATTGAGTATCTGGAGAAGACTCTGCCTTTCAGTCGTTCTTACTACTGGATAGGAATCCGGAAGATAGGAGGAATATGGACGTGGGTGGGAACCAACAAATCTCTCACTGAAGAAGCAGAGAACTGGGGAGATGGTGAGCCCAACAACAAGAAGAACAAGGAGGACTGCGTGGAGATCTATATCAAGAGAAACAAAGATGCAGGCAAATGGAACGATGACGCCTGCCACAAACTAAAGGCAGCCCTCTGTTACACAGCTTCTTGCCAGCCCTGGTCATGCAGTGGCCATGGAGAATGTGTAGAAATCATCAATAATTACACCTGCAACTGTGATGTGGGGTACTATGGGCCCCAGTGTCAGTTTGTGATTCAGTGTGAGCCTTTGGAGGCCCCAGAGCTGGGTACCATGGACTGTACTCACTCTTTGGGAAACTTCAGCTTCAGCTCACAGTGTGCCTTCAGCTGCTCTGAAGGAACAAACTTAACTGGGATTGAAGAAACCACCTGTGGACCATTTGGAAACTGGTCATCTCCAGAACCAACCTGTCAAGTGATTCAGTGTGAGCCTCTATCAGCACCAGATTTGGGGATCATGAACTGTAGCCATCCCCTGGCCAGCTTCAGCTTTACCTCTGCATGTACCTTCATCTGCTCAGAAGGAACTGAGTTAATTGGGAAGAAGAAAACCATTTGTGAATCATCTGGAATCTGGTCAAATCCTAGTCCAATATGTCAAAAATTGGACAAAAGTTTCTCAATGATTAAGGAGGGTGATTATAACCCCCTCTTCATTCCAGTGGCAGTCATGGTTACTGCATTCTCTGGGTTGGCATTTATCATTTGGCTGGCAAGGAGATTAAAAAAAGGCAAGAAATCCAAGAGAAGTATGAATGACCCATATTAAATCGCCCTTGGTGAAAGAAAATTCTTGGAATACTAAAAATCATGAGATCCTTTAAATCCTTCCATGAAACGTTTTGTGTGGTGGCACCTCCTACGTCAAACATGAAGTGTGTTTCCTTCAGTGCATCTGGGAAGATTTCTACCTGACCAACAGTTCCTTCAGCTTCCATTTCGCCCCTCATTTATCCCTCAACCCCCAGCCCACAGGTGTTTATACAGCTCAGCTTTTTGTCTTTTCTGAGGAGAAACAAATAAGACCATAAAGGGAAAGGATTCATGTGGAATATAAAGATGGCTGACTTTGCTCTTTCTTGACTCTTGTTTTCAGTTTCAATTCAGTGCTGTACTTGATGACAGACACTTCTAAATGAAGTGCAAATTTGATACATATGTGAATATGGACTCAGTTTTCTTGCAGATCAAATTTCACGTCGTCTTCTGTATACTGTGGAGGTACACTCTTATAGAAAGTTCAAAAAGTCTACGCTCTCCTTTCTTTCTAACTCCAGTGAAGTAATGGGGTCCTGCTCAAGTTGAAAGAGTCCTATTTGCACTGTAGCCTCGCCGTCTGTGAATTGGACCATCCTATTTAACTGGCTTCAGCCTCCCCACCTTCTTCAGCCACCTCTCTTTTTCAGTTGGCTGACTTCCACACCTAGCATCTCATGAGTGCCAAGCAAAAGGAGAGAAGAGAGAAATAGCCTGCGCTGTTTTTTAGTTTGGGGGTTTTGCTGTTTCCTTTTATGAGACCCATTCCTATTTCTTATAGTCAATGTTTCTTTTATCACGATATTATTAGTAAGAAAACATCACTGAAATGCTAGCTGCAAGTGACATCTCTTTGATGTCATATGGAAGAGTTAAAACAGGTGGAGAAATTCCTTGATTCACAATGAAATGCTCTCCTTTCCCCTGCCCCCAGACCTTTTATCCGACTTACCTAGATTCTACATATTCTTTAAATTTCATCTCAGGCCTCCCTCAACCCCACCACTTCTTTTATAACTAGTCCTTTACTAATCCAACCCATGATGAGCTCCTCTTCCTGGCTTCTTACTGAAAGGTTACCCTGTAACATGCAATTTTGCATTTGAATAAAGCCTGCTTTTTAAGTGTT
673
PRIMER_START_CODON_POSITION=49
674
PRIMER_LEFT_EXPLAIN=considered 19864, would not amplify any of the ORF 16423, low tm 794, high tm 1127, high end compl 2, long poly-x seq 23,high 3' stability 37, ok 1458
675
PRIMER_RIGHT_EXPLAIN=considered 19472, GC content failed 127, low tm 6190, high tm 5041, high end compl 1, long poly-x seq 45,high 3' stability 67, ok 8001
676
PRIMER_PAIR_EXPLAIN=considered 57, high end compl 2, ok 55
677
PRIMER_STOP_CODON_POSITION=1204
678
PRIMER_PAIR_PENALTY=6.7884
679
PRIMER_LEFT_PENALTY=1.909478
680
PRIMER_RIGHT_PENALTY=4.878922
681
PRIMER_LEFT_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
682
PRIMER_RIGHT_SEQUENCE=GGGCGATTTAATATGGGTCA
683
PRIMER_LEFT=49,21
684
PRIMER_RIGHT=1213,20
685
PRIMER_LEFT_TM=59.091
686
PRIMER_RIGHT_TM=59.621
687
PRIMER_LEFT_GC_PERCENT=47.619
688
PRIMER_RIGHT_GC_PERCENT=45.000
689
PRIMER_LEFT_SELF_ANY=6.00
690
PRIMER_RIGHT_SELF_ANY=5.00
691
PRIMER_LEFT_SELF_END=2.00
692
PRIMER_RIGHT_SELF_END=1.00
693
PRIMER_LEFT_POSITION_PENALTY=0.000000
694
PRIMER_RIGHT_POSITION_PENALTY=4.500000
695
PRIMER_LEFT_END_STABILITY=5.7000
696
PRIMER_RIGHT_END_STABILITY=7.9000
697
PRIMER_PAIR_COMPL_ANY=2.00
698
PRIMER_PAIR_COMPL_END=1.00
699
PRIMER_PRODUCT_SIZE=1165
700
PRIMER_PAIR_PENALTY_1=6.9188
701
PRIMER_LEFT_1_PENALTY=2.039879
702
PRIMER_RIGHT_1_PENALTY=4.878922
703
PRIMER_LEFT_1_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG
704
PRIMER_RIGHT_1_SEQUENCE=GGGCGATTTAATATGGGTCA
705
PRIMER_LEFT_1=49,22
706
PRIMER_RIGHT_1=1213,20
707
PRIMER_LEFT_1_TM=60.040
708
PRIMER_RIGHT_1_TM=59.621
709
PRIMER_LEFT_1_GC_PERCENT=50.000
710
PRIMER_RIGHT_1_GC_PERCENT=45.000
711
PRIMER_LEFT_1_SELF_ANY=6.00
712
PRIMER_RIGHT_1_SELF_ANY=5.00
713
PRIMER_LEFT_1_SELF_END=2.00
714
PRIMER_RIGHT_1_SELF_END=1.00
715
PRIMER_LEFT_1_POSITION_PENALTY=0.000000
716
PRIMER_RIGHT_1_POSITION_PENALTY=4.500000
717
PRIMER_LEFT_1_END_STABILITY=5.7000
718
PRIMER_RIGHT_1_END_STABILITY=7.9000
719
PRIMER_PAIR_1_COMPL_ANY=2.00
720
PRIMER_PAIR_1_COMPL_END=0.00
721
PRIMER_PRODUCT_SIZE_1=1165
722
PRIMER_PAIR_PENALTY_2=7.5160
723
PRIMER_LEFT_2_PENALTY=1.909478
724
PRIMER_RIGHT_2_PENALTY=5.606474
725
PRIMER_LEFT_2_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
726
PRIMER_RIGHT_2_SEQUENCE=GGGCGATTTAATATGGGTCAT
727
PRIMER_LEFT_2=49,21
728
PRIMER_RIGHT_2=1213,21
729
PRIMER_LEFT_2_TM=59.091
730
PRIMER_RIGHT_2_TM=59.894
731
PRIMER_LEFT_2_GC_PERCENT=47.619
732
PRIMER_RIGHT_2_GC_PERCENT=42.857
733
PRIMER_LEFT_2_SELF_ANY=6.00
734
PRIMER_RIGHT_2_SELF_ANY=5.00
735
PRIMER_LEFT_2_SELF_END=2.00
736
PRIMER_RIGHT_2_SELF_END=3.00
737
PRIMER_LEFT_2_POSITION_PENALTY=0.000000
738
PRIMER_RIGHT_2_POSITION_PENALTY=4.500000
739
PRIMER_LEFT_2_END_STABILITY=5.7000
740
PRIMER_RIGHT_2_END_STABILITY=6.3000
741
PRIMER_PAIR_2_COMPL_ANY=3.00
742
PRIMER_PAIR_2_COMPL_END=2.00
743
PRIMER_PRODUCT_SIZE_2=1165
744
PRIMER_PAIR_PENALTY_3=7.6464
745
PRIMER_LEFT_3_PENALTY=2.039879
746
PRIMER_RIGHT_3_PENALTY=5.606474
747
PRIMER_LEFT_3_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG
748
PRIMER_RIGHT_3_SEQUENCE=GGGCGATTTAATATGGGTCAT
749
PRIMER_LEFT_3=49,22
750
PRIMER_RIGHT_3=1213,21
751
PRIMER_LEFT_3_TM=60.040
752
PRIMER_RIGHT_3_TM=59.894
753
PRIMER_LEFT_3_GC_PERCENT=50.000
754
PRIMER_RIGHT_3_GC_PERCENT=42.857
755
PRIMER_LEFT_3_SELF_ANY=6.00
756
PRIMER_RIGHT_3_SELF_ANY=5.00
757
PRIMER_LEFT_3_SELF_END=2.00
758
PRIMER_RIGHT_3_SELF_END=3.00
759
PRIMER_LEFT_3_POSITION_PENALTY=0.000000
760
PRIMER_RIGHT_3_POSITION_PENALTY=4.500000
761
PRIMER_LEFT_3_END_STABILITY=5.7000
762
PRIMER_RIGHT_3_END_STABILITY=6.3000
763
PRIMER_PAIR_3_COMPL_ANY=3.00
764
PRIMER_PAIR_3_COMPL_END=1.00
765
PRIMER_PRODUCT_SIZE_3=1165
766
PRIMER_PAIR_PENALTY_4=8.3537
767
PRIMER_LEFT_4_PENALTY=1.909478
768
PRIMER_RIGHT_4_PENALTY=6.444219
769
PRIMER_LEFT_4_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
770
PRIMER_RIGHT_4_SEQUENCE=GGCGATTTAATATGGGTCATTC
771
PRIMER_LEFT_4=49,21
772
PRIMER_RIGHT_4=1212,22
773
PRIMER_LEFT_4_TM=59.091
774
PRIMER_RIGHT_4_TM=59.556
775
PRIMER_LEFT_4_GC_PERCENT=47.619
776
PRIMER_RIGHT_4_GC_PERCENT=40.909
777
PRIMER_LEFT_4_SELF_ANY=6.00
778
PRIMER_RIGHT_4_SELF_ANY=5.00
779
PRIMER_LEFT_4_SELF_END=2.00
780
PRIMER_RIGHT_4_SELF_END=0.00
781
PRIMER_LEFT_4_POSITION_PENALTY=0.000000
782
PRIMER_RIGHT_4_POSITION_PENALTY=4.000000
783
PRIMER_LEFT_4_END_STABILITY=5.7000
784
PRIMER_RIGHT_4_END_STABILITY=6.9000
785
PRIMER_PAIR_4_COMPL_ANY=3.00
786
PRIMER_PAIR_4_COMPL_END=3.00
787
PRIMER_PRODUCT_SIZE_4=1164
788
PRIMER_PAIR_PENALTY_5=8.4316
789
PRIMER_LEFT_5_PENALTY=1.909478
790
PRIMER_RIGHT_5_PENALTY=6.522147
791
PRIMER_LEFT_5_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
792
PRIMER_RIGHT_5_SEQUENCE=AGGGCGATTTAATATGGGTCA
793
PRIMER_LEFT_5=49,21
794
PRIMER_RIGHT_5=1214,21
795
PRIMER_LEFT_5_TM=59.091
796
PRIMER_RIGHT_5_TM=60.522
797
PRIMER_LEFT_5_GC_PERCENT=47.619
798
PRIMER_RIGHT_5_GC_PERCENT=42.857
799
PRIMER_LEFT_5_SELF_ANY=6.00
800
PRIMER_RIGHT_5_SELF_ANY=5.00
801
PRIMER_LEFT_5_SELF_END=2.00
802
PRIMER_RIGHT_5_SELF_END=1.00
803
PRIMER_LEFT_5_POSITION_PENALTY=0.000000
804
PRIMER_RIGHT_5_POSITION_PENALTY=5.000000
805
PRIMER_LEFT_5_END_STABILITY=5.7000
806
PRIMER_RIGHT_5_END_STABILITY=7.9000
807
PRIMER_PAIR_5_COMPL_ANY=2.00
808
PRIMER_PAIR_5_COMPL_END=1.00
809
PRIMER_PRODUCT_SIZE_5=1166
810
PRIMER_PAIR_PENALTY_6=8.4841
811
PRIMER_LEFT_6_PENALTY=2.039879
812
PRIMER_RIGHT_6_PENALTY=6.444219
813
PRIMER_LEFT_6_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG
814
PRIMER_RIGHT_6_SEQUENCE=GGCGATTTAATATGGGTCATTC
815
PRIMER_LEFT_6=49,22
816
PRIMER_RIGHT_6=1212,22
817
PRIMER_LEFT_6_TM=60.040
818
PRIMER_RIGHT_6_TM=59.556
819
PRIMER_LEFT_6_GC_PERCENT=50.000
820
PRIMER_RIGHT_6_GC_PERCENT=40.909
821
PRIMER_LEFT_6_SELF_ANY=6.00
822
PRIMER_RIGHT_6_SELF_ANY=5.00
823
PRIMER_LEFT_6_SELF_END=2.00
824
PRIMER_RIGHT_6_SELF_END=0.00
825
PRIMER_LEFT_6_POSITION_PENALTY=0.000000
826
PRIMER_RIGHT_6_POSITION_PENALTY=4.000000
827
PRIMER_LEFT_6_END_STABILITY=5.7000
828
PRIMER_RIGHT_6_END_STABILITY=6.9000
829
PRIMER_PAIR_6_COMPL_ANY=3.00
830
PRIMER_PAIR_6_COMPL_END=2.00
831
PRIMER_PRODUCT_SIZE_6=1164
832
PRIMER_PAIR_PENALTY_7=8.5620
833
PRIMER_LEFT_7_PENALTY=2.039879
834
PRIMER_RIGHT_7_PENALTY=6.522147
835
PRIMER_LEFT_7_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG
836
PRIMER_RIGHT_7_SEQUENCE=AGGGCGATTTAATATGGGTCA
837
PRIMER_LEFT_7=49,22
838
PRIMER_RIGHT_7=1214,21
839
PRIMER_LEFT_7_TM=60.040
840
PRIMER_RIGHT_7_TM=60.522
841
PRIMER_LEFT_7_GC_PERCENT=50.000
842
PRIMER_RIGHT_7_GC_PERCENT=42.857
843
PRIMER_LEFT_7_SELF_ANY=6.00
844
PRIMER_RIGHT_7_SELF_ANY=5.00
845
PRIMER_LEFT_7_SELF_END=2.00
846
PRIMER_RIGHT_7_SELF_END=1.00
847
PRIMER_LEFT_7_POSITION_PENALTY=0.000000
848
PRIMER_RIGHT_7_POSITION_PENALTY=5.000000
849
PRIMER_LEFT_7_END_STABILITY=5.7000
850
PRIMER_RIGHT_7_END_STABILITY=7.9000
851
PRIMER_PAIR_7_COMPL_ANY=2.00
852
PRIMER_PAIR_7_COMPL_END=0.00
853
PRIMER_PRODUCT_SIZE_7=1166
854
PRIMER_PAIR_PENALTY_8=8.5798
855
PRIMER_LEFT_8_PENALTY=1.909478
856
PRIMER_RIGHT_8_PENALTY=6.670364
857
PRIMER_LEFT_8_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
858
PRIMER_RIGHT_8_SEQUENCE=GCGATTTAATATGGGTCATTCA
859
PRIMER_LEFT_8=49,21
860
PRIMER_RIGHT_8=1211,22
861
PRIMER_LEFT_8_TM=59.091
862
PRIMER_RIGHT_8_TM=58.830
863
PRIMER_LEFT_8_GC_PERCENT=47.619
864
PRIMER_RIGHT_8_GC_PERCENT=36.364
865
PRIMER_LEFT_8_SELF_ANY=6.00
866
PRIMER_RIGHT_8_SELF_ANY=5.00
867
PRIMER_LEFT_8_SELF_END=2.00
868
PRIMER_RIGHT_8_SELF_END=1.00
869
PRIMER_LEFT_8_POSITION_PENALTY=0.000000
870
PRIMER_RIGHT_8_POSITION_PENALTY=3.500000
871
PRIMER_LEFT_8_END_STABILITY=5.7000
872
PRIMER_RIGHT_8_END_STABILITY=6.9000
873
PRIMER_PAIR_8_COMPL_ANY=3.00
874
PRIMER_PAIR_8_COMPL_END=2.00
875
PRIMER_PRODUCT_SIZE_8=1163
876
PRIMER_PAIR_PENALTY_9=8.6317
877
PRIMER_LEFT_9_PENALTY=1.909478
878
PRIMER_RIGHT_9_PENALTY=6.722200
879
PRIMER_LEFT_9_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
880
PRIMER_RIGHT_9_SEQUENCE=CAAGGGCGATTTAATATGGG
881
PRIMER_LEFT_9=49,21
882
PRIMER_RIGHT_9=1216,20
883
PRIMER_LEFT_9_TM=59.091
884
PRIMER_RIGHT_9_TM=59.278
885
PRIMER_LEFT_9_GC_PERCENT=47.619
886
PRIMER_RIGHT_9_GC_PERCENT=45.000
887
PRIMER_LEFT_9_SELF_ANY=6.00
888
PRIMER_RIGHT_9_SELF_ANY=5.00
889
PRIMER_LEFT_9_SELF_END=2.00
890
PRIMER_RIGHT_9_SELF_END=2.00
891
PRIMER_LEFT_9_POSITION_PENALTY=0.000000
892
PRIMER_RIGHT_9_POSITION_PENALTY=6.000000
893
PRIMER_LEFT_9_END_STABILITY=5.7000
894
PRIMER_RIGHT_9_END_STABILITY=9.6000
895
PRIMER_PAIR_9_COMPL_ANY=2.00
896
PRIMER_PAIR_9_COMPL_END=0.00
897
PRIMER_PRODUCT_SIZE_9=1168
898
PRIMER_PAIR_PENALTY_10=8.7102
899
PRIMER_LEFT_10_PENALTY=2.039879
900
PRIMER_RIGHT_10_PENALTY=6.670364
901
PRIMER_LEFT_10_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG
902
PRIMER_RIGHT_10_SEQUENCE=GCGATTTAATATGGGTCATTCA
903
PRIMER_LEFT_10=49,22
904
PRIMER_RIGHT_10=1211,22
905
PRIMER_LEFT_10_TM=60.040
906
PRIMER_RIGHT_10_TM=58.830
907
PRIMER_LEFT_10_GC_PERCENT=50.000
908
PRIMER_RIGHT_10_GC_PERCENT=36.364
909
PRIMER_LEFT_10_SELF_ANY=6.00
910
PRIMER_RIGHT_10_SELF_ANY=5.00
911
PRIMER_LEFT_10_SELF_END=2.00
912
PRIMER_RIGHT_10_SELF_END=1.00
913
PRIMER_LEFT_10_POSITION_PENALTY=0.000000
914
PRIMER_RIGHT_10_POSITION_PENALTY=3.500000
915
PRIMER_LEFT_10_END_STABILITY=5.7000
916
PRIMER_RIGHT_10_END_STABILITY=6.9000
917
PRIMER_PAIR_10_COMPL_ANY=3.00
918
PRIMER_PAIR_10_COMPL_END=1.00
919
PRIMER_PRODUCT_SIZE_10=1163
920
PRIMER_PAIR_PENALTY_11=8.7621
921
PRIMER_LEFT_11_PENALTY=2.039879
922
PRIMER_RIGHT_11_PENALTY=6.722200
923
PRIMER_LEFT_11_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG
924
PRIMER_RIGHT_11_SEQUENCE=CAAGGGCGATTTAATATGGG
925
PRIMER_LEFT_11=49,22
926
PRIMER_RIGHT_11=1216,20
927
PRIMER_LEFT_11_TM=60.040
928
PRIMER_RIGHT_11_TM=59.278
929
PRIMER_LEFT_11_GC_PERCENT=50.000
930
PRIMER_RIGHT_11_GC_PERCENT=45.000
931
PRIMER_LEFT_11_SELF_ANY=6.00
932
PRIMER_RIGHT_11_SELF_ANY=5.00
933
PRIMER_LEFT_11_SELF_END=2.00
934
PRIMER_RIGHT_11_SELF_END=2.00
935
PRIMER_LEFT_11_POSITION_PENALTY=0.000000
936
PRIMER_RIGHT_11_POSITION_PENALTY=6.000000
937
PRIMER_LEFT_11_END_STABILITY=5.7000
938
PRIMER_RIGHT_11_END_STABILITY=9.6000
939
PRIMER_PAIR_11_COMPL_ANY=2.00
940
PRIMER_PAIR_11_COMPL_END=0.00
941
PRIMER_PRODUCT_SIZE_11=1168
942
PRIMER_PAIR_PENALTY_12=8.9584
943
PRIMER_LEFT_12_PENALTY=1.909478
944
PRIMER_RIGHT_12_PENALTY=7.048962
945
PRIMER_LEFT_12_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
946
PRIMER_RIGHT_12_SEQUENCE=CAAGGGCGATTTAATATGGGT
947
PRIMER_LEFT_12=49,21
948
PRIMER_RIGHT_12=1216,21
949
PRIMER_LEFT_12_TM=59.091
950
PRIMER_RIGHT_12_TM=60.049
951
PRIMER_LEFT_12_GC_PERCENT=47.619
952
PRIMER_RIGHT_12_GC_PERCENT=42.857
953
PRIMER_LEFT_12_SELF_ANY=6.00
954
PRIMER_RIGHT_12_SELF_ANY=5.00
955
PRIMER_LEFT_12_SELF_END=2.00
956
PRIMER_RIGHT_12_SELF_END=0.00
957
PRIMER_LEFT_12_POSITION_PENALTY=0.000000
958
PRIMER_RIGHT_12_POSITION_PENALTY=6.000000
959
PRIMER_LEFT_12_END_STABILITY=5.7000
960
PRIMER_RIGHT_12_END_STABILITY=9.4000
961
PRIMER_PAIR_12_COMPL_ANY=2.00
962
PRIMER_PAIR_12_COMPL_END=1.00
963
PRIMER_PRODUCT_SIZE_12=1168
964
PRIMER_PAIR_PENALTY_13=9.0139
965
PRIMER_LEFT_13_PENALTY=1.909478
966
PRIMER_RIGHT_13_PENALTY=7.104387
967
PRIMER_LEFT_13_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
968
PRIMER_RIGHT_13_SEQUENCE=AGGGCGATTTAATATGGGTC
969
PRIMER_LEFT_13=49,21
970
PRIMER_RIGHT_13=1214,20
971
PRIMER_LEFT_13_TM=59.091
972
PRIMER_RIGHT_13_TM=57.896
973
PRIMER_LEFT_13_GC_PERCENT=47.619
974
PRIMER_RIGHT_13_GC_PERCENT=45.000
975
PRIMER_LEFT_13_SELF_ANY=6.00
976
PRIMER_RIGHT_13_SELF_ANY=5.00
977
PRIMER_LEFT_13_SELF_END=2.00
978
PRIMER_RIGHT_13_SELF_END=2.00
979
PRIMER_LEFT_13_POSITION_PENALTY=0.000000
980
PRIMER_RIGHT_13_POSITION_PENALTY=5.000000
981
PRIMER_LEFT_13_END_STABILITY=5.7000
982
PRIMER_RIGHT_13_END_STABILITY=9.1000
983
PRIMER_PAIR_13_COMPL_ANY=2.00
984
PRIMER_PAIR_13_COMPL_END=2.00
985
PRIMER_PRODUCT_SIZE_13=1166
986
PRIMER_PAIR_PENALTY_14=9.0672
987
PRIMER_LEFT_14_PENALTY=1.909478
988
PRIMER_RIGHT_14_PENALTY=7.157689
989
PRIMER_LEFT_14_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
990
PRIMER_RIGHT_14_SEQUENCE=GGCGATTTAATATGGGTCATT
991
PRIMER_LEFT_14=49,21
992
PRIMER_RIGHT_14=1212,21
993
PRIMER_LEFT_14_TM=59.091
994
PRIMER_RIGHT_14_TM=57.842
995
PRIMER_LEFT_14_GC_PERCENT=47.619
996
PRIMER_RIGHT_14_GC_PERCENT=38.095
997
PRIMER_LEFT_14_SELF_ANY=6.00
998
PRIMER_RIGHT_14_SELF_ANY=5.00
999
PRIMER_LEFT_14_SELF_END=2.00
1000
PRIMER_RIGHT_14_SELF_END=1.00
1001
PRIMER_LEFT_14_POSITION_PENALTY=0.000000
1002
PRIMER_RIGHT_14_POSITION_PENALTY=4.000000
1003
PRIMER_LEFT_14_END_STABILITY=5.7000
1004
PRIMER_RIGHT_14_END_STABILITY=6.9000
1005
PRIMER_PAIR_14_COMPL_ANY=3.00
1006
PRIMER_PAIR_14_COMPL_END=2.00
1007
PRIMER_PRODUCT_SIZE_14=1164
1008
PRIMER_PAIR_PENALTY_15=9.0888
1009
PRIMER_LEFT_15_PENALTY=2.039879
1010
PRIMER_RIGHT_15_PENALTY=7.048962
1011
PRIMER_LEFT_15_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG
1012
PRIMER_RIGHT_15_SEQUENCE=CAAGGGCGATTTAATATGGGT
1013
PRIMER_LEFT_15=49,22
1014
PRIMER_RIGHT_15=1216,21
1015
PRIMER_LEFT_15_TM=60.040
1016
PRIMER_RIGHT_15_TM=60.049
1017
PRIMER_LEFT_15_GC_PERCENT=50.000
1018
PRIMER_RIGHT_15_GC_PERCENT=42.857
1019
PRIMER_LEFT_15_SELF_ANY=6.00
1020
PRIMER_RIGHT_15_SELF_ANY=5.00
1021
PRIMER_LEFT_15_SELF_END=2.00
1022
PRIMER_RIGHT_15_SELF_END=0.00
1023
PRIMER_LEFT_15_POSITION_PENALTY=0.000000
1024
PRIMER_RIGHT_15_POSITION_PENALTY=6.000000
1025
PRIMER_LEFT_15_END_STABILITY=5.7000
1026
PRIMER_RIGHT_15_END_STABILITY=9.4000
1027
PRIMER_PAIR_15_COMPL_ANY=2.00
1028
PRIMER_PAIR_15_COMPL_END=0.00
1029
PRIMER_PRODUCT_SIZE_15=1168
1030
PRIMER_PAIR_PENALTY_16=9.1443
1031
PRIMER_LEFT_16_PENALTY=2.039879
1032
PRIMER_RIGHT_16_PENALTY=7.104387
1033
PRIMER_LEFT_16_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG
1034
PRIMER_RIGHT_16_SEQUENCE=AGGGCGATTTAATATGGGTC
1035
PRIMER_LEFT_16=49,22
1036
PRIMER_RIGHT_16=1214,20
1037
PRIMER_LEFT_16_TM=60.040
1038
PRIMER_RIGHT_16_TM=57.896
1039
PRIMER_LEFT_16_GC_PERCENT=50.000
1040
PRIMER_RIGHT_16_GC_PERCENT=45.000
1041
PRIMER_LEFT_16_SELF_ANY=6.00
1042
PRIMER_RIGHT_16_SELF_ANY=5.00
1043
PRIMER_LEFT_16_SELF_END=2.00
1044
PRIMER_RIGHT_16_SELF_END=2.00
1045
PRIMER_LEFT_16_POSITION_PENALTY=0.000000
1046
PRIMER_RIGHT_16_POSITION_PENALTY=5.000000
1047
PRIMER_LEFT_16_END_STABILITY=5.7000
1048
PRIMER_RIGHT_16_END_STABILITY=9.1000
1049
PRIMER_PAIR_16_COMPL_ANY=2.00
1050
PRIMER_PAIR_16_COMPL_END=1.00
1051
PRIMER_PRODUCT_SIZE_16=1166
1052
PRIMER_PAIR_PENALTY_17=9.1976
1053
PRIMER_LEFT_17_PENALTY=2.039879
1054
PRIMER_RIGHT_17_PENALTY=7.157689
1055
PRIMER_LEFT_17_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG
1056
PRIMER_RIGHT_17_SEQUENCE=GGCGATTTAATATGGGTCATT
1057
PRIMER_LEFT_17=49,22
1058
PRIMER_RIGHT_17=1212,21
1059
PRIMER_LEFT_17_TM=60.040
1060
PRIMER_RIGHT_17_TM=57.842
1061
PRIMER_LEFT_17_GC_PERCENT=50.000
1062
PRIMER_RIGHT_17_GC_PERCENT=38.095
1063
PRIMER_LEFT_17_SELF_ANY=6.00
1064
PRIMER_RIGHT_17_SELF_ANY=5.00
1065
PRIMER_LEFT_17_SELF_END=2.00
1066
PRIMER_RIGHT_17_SELF_END=1.00
1067
PRIMER_LEFT_17_POSITION_PENALTY=0.000000
1068
PRIMER_RIGHT_17_POSITION_PENALTY=4.000000
1069
PRIMER_LEFT_17_END_STABILITY=5.7000
1070
PRIMER_RIGHT_17_END_STABILITY=6.9000
1071
PRIMER_PAIR_17_COMPL_ANY=3.00
1072
PRIMER_PAIR_17_COMPL_END=1.00
1073
PRIMER_PRODUCT_SIZE_17=1164
1074
PRIMER_PAIR_PENALTY_18=9.2077
1075
PRIMER_LEFT_18_PENALTY=1.909478
1076
PRIMER_RIGHT_18_PENALTY=7.298213
1077
PRIMER_LEFT_18_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
1078
PRIMER_RIGHT_18_SEQUENCE=AAGGGCGATTTAATATGGGTC
1079
PRIMER_LEFT_18=49,21
1080
PRIMER_RIGHT_18=1215,21
1081
PRIMER_LEFT_18_TM=59.091
1082
PRIMER_RIGHT_18_TM=59.202
1083
PRIMER_LEFT_18_GC_PERCENT=47.619
1084
PRIMER_RIGHT_18_GC_PERCENT=42.857
1085
PRIMER_LEFT_18_SELF_ANY=6.00
1086
PRIMER_RIGHT_18_SELF_ANY=5.00
1087
PRIMER_LEFT_18_SELF_END=2.00
1088
PRIMER_RIGHT_18_SELF_END=2.00
1089
PRIMER_LEFT_18_POSITION_PENALTY=0.000000
1090
PRIMER_RIGHT_18_POSITION_PENALTY=5.500000
1091
PRIMER_LEFT_18_END_STABILITY=5.7000
1092
PRIMER_RIGHT_18_END_STABILITY=9.1000
1093
PRIMER_PAIR_18_COMPL_ANY=2.00
1094
PRIMER_PAIR_18_COMPL_END=2.00
1095
PRIMER_PRODUCT_SIZE_18=1167
1096
PRIMER_PAIR_PENALTY_19=9.2811
1097
PRIMER_LEFT_19_PENALTY=1.909478
1098
PRIMER_RIGHT_19_PENALTY=7.371606
1099
PRIMER_LEFT_19_SEQUENCE=ATGGGCTGCAGAAGAACTAGA
1100
PRIMER_RIGHT_19_SEQUENCE=GCGATTTAATATGGGTCATTCAT
1101
PRIMER_LEFT_19=49,21
1102
PRIMER_RIGHT_19=1211,23
1103
PRIMER_LEFT_19_TM=59.091
1104
PRIMER_RIGHT_19_TM=59.128
1105
PRIMER_LEFT_19_GC_PERCENT=47.619
1106
PRIMER_RIGHT_19_GC_PERCENT=34.783
1107
PRIMER_LEFT_19_SELF_ANY=6.00
1108
PRIMER_RIGHT_19_SELF_ANY=5.00
1109
PRIMER_LEFT_19_SELF_END=2.00
1110
PRIMER_RIGHT_19_SELF_END=3.00
1111
PRIMER_LEFT_19_POSITION_PENALTY=0.000000
1112
PRIMER_RIGHT_19_POSITION_PENALTY=3.500000
1113
PRIMER_LEFT_19_END_STABILITY=5.7000
1114
PRIMER_RIGHT_19_END_STABILITY=6.9000
1115
PRIMER_PAIR_19_COMPL_ANY=3.00
1116
PRIMER_PAIR_19_COMPL_END=2.00
1117
PRIMER_PRODUCT_SIZE_19=1163
1118
=
1119
PRIMER_SEQUENCE_ID=L40146/UBC4
1120
PRIMER_START_CODON_POSITION=0
1121
SEQUENCE=ATGGCGCTGAAAAGAATCCACAAGGAATTGAATGATCTGGCACGGGACCCTCCAGCACAGTGTTCAGCAGGTCCTGTTGGAGATGATATGTTCCATTGGCAAGCTACAATAATGGGGCCAAATGACAGTCCCTATCAGGGTGGAGTATTTTTCTTGACAATTCATTTCCCAACAGATTACCCCTTCAAACCACCTAAGGTTGCATTTACAACAAGAATTTATCATCCAAATATTAACAGTAATGGCAGCATTTGTCTTGATATTCTACGATCACAGTGGTCTCCAGCACTAACTATTTCAAAAGTACTCTTGTCCATCTGTTCTCTGTTGTGTGATCCCAATCCAGATGATCCTTTAGTGCCTGAGATTGCTCGGATCTACCAAACAGATAGAGAAAAGTACAACAGAATAGCTCGGGAATGGACTCAGAAGTATGCGATGTAA
1122
PRIMER_LEFT_EXPLAIN=considered 3285, would not amplify any of the ORF 2182, GC content failed 11, low tm 330, high tm 255, high end compl 5,high 3' stability 7, ok 495
1123
PRIMER_RIGHT_EXPLAIN=considered 3267, GC content failed 36, low tm 1201, high tm 523,high 3' stability 8, ok 1499
1124
PRIMER_PAIR_EXPLAIN=considered 81, high end compl 35, ok 46
1125
PRIMER_STOP_CODON_POSITION=441
1126
PRIMER_PAIR_PENALTY=6.8208
1127
PRIMER_LEFT_PENALTY=4.584153
1128
PRIMER_RIGHT_PENALTY=2.236656
1129
PRIMER_LEFT_SEQUENCE=ATGGCGCTGAAAAGAATCCAC
1130
PRIMER_RIGHT_SEQUENCE=ACATCGCATACTTCTGAGTCCA
1131
PRIMER_LEFT=0,21
1132
PRIMER_RIGHT=441,22
1133
PRIMER_LEFT_TM=63.584
1134
PRIMER_RIGHT_TM=59.763
1135
PRIMER_LEFT_GC_PERCENT=47.619
1136
PRIMER_RIGHT_GC_PERCENT=45.455
1137
PRIMER_LEFT_SELF_ANY=4.00
1138
PRIMER_RIGHT_SELF_ANY=3.00
1139
PRIMER_LEFT_SELF_END=0.00
1140
PRIMER_RIGHT_SELF_END=1.00
1141
PRIMER_LEFT_POSITION_PENALTY=0.000000
1142
PRIMER_RIGHT_POSITION_PENALTY=0.000000
1143
PRIMER_LEFT_END_STABILITY=7.9000
1144
PRIMER_RIGHT_END_STABILITY=7.9000
1145
PRIMER_PAIR_COMPL_ANY=5.00
1146
PRIMER_PAIR_COMPL_END=3.00
1147
PRIMER_PRODUCT_SIZE=442
1148
PRIMER_PAIR_PENALTY_1=8.3015
1149
PRIMER_LEFT_1_PENALTY=4.584153
1150
PRIMER_RIGHT_1_PENALTY=3.717312
1151
PRIMER_LEFT_1_SEQUENCE=ATGGCGCTGAAAAGAATCCAC
1152
PRIMER_RIGHT_1_SEQUENCE=TACATCGCATACTTCTGAGTCCA
1153
PRIMER_LEFT_1=0,21
1154
PRIMER_RIGHT_1=442,23
1155
PRIMER_LEFT_1_TM=63.584
1156
PRIMER_RIGHT_1_TM=59.783
1157
PRIMER_LEFT_1_GC_PERCENT=47.619
1158
PRIMER_RIGHT_1_GC_PERCENT=43.478
1159
PRIMER_LEFT_1_SELF_ANY=4.00
1160
PRIMER_RIGHT_1_SELF_ANY=3.00
1161
PRIMER_LEFT_1_SELF_END=0.00
1162
PRIMER_RIGHT_1_SELF_END=1.00
1163
PRIMER_LEFT_1_POSITION_PENALTY=0.000000
1164
PRIMER_RIGHT_1_POSITION_PENALTY=0.500000
1165
PRIMER_LEFT_1_END_STABILITY=7.9000
1166
PRIMER_RIGHT_1_END_STABILITY=7.9000
1167
PRIMER_PAIR_1_COMPL_ANY=5.00
1168
PRIMER_PAIR_1_COMPL_END=3.00
1169
PRIMER_PRODUCT_SIZE_1=443
1170
PRIMER_PAIR_PENALTY_2=8.4940
1171
PRIMER_LEFT_2_PENALTY=2.909495
1172
PRIMER_RIGHT_2_PENALTY=5.584511
1173
PRIMER_LEFT_2_SEQUENCE=ATGGCGCTGAAAAGAATCCA
1174
PRIMER_RIGHT_2_SEQUENCE=TTACATCGCATACTTCTGAGTCC
1175
PRIMER_LEFT_2=0,20
1176
PRIMER_RIGHT_2=443,23
1177
PRIMER_LEFT_2_TM=62.909
1178
PRIMER_RIGHT_2_TM=58.415
1179
PRIMER_LEFT_2_GC_PERCENT=45.000
1180
PRIMER_RIGHT_2_GC_PERCENT=43.478
1181
PRIMER_LEFT_2_SELF_ANY=4.00
1182
PRIMER_RIGHT_2_SELF_ANY=3.00
1183
PRIMER_LEFT_2_SELF_END=0.00
1184
PRIMER_RIGHT_2_SELF_END=1.00
1185
PRIMER_LEFT_2_POSITION_PENALTY=0.000000
1186
PRIMER_RIGHT_2_POSITION_PENALTY=1.000000
1187
PRIMER_LEFT_2_END_STABILITY=8.1000
1188
PRIMER_RIGHT_2_END_STABILITY=7.6000
1189
PRIMER_PAIR_2_COMPL_ANY=5.00
1190
PRIMER_PAIR_2_COMPL_END=3.00
1191
PRIMER_PRODUCT_SIZE_2=444
1192
PRIMER_PAIR_PENALTY_3=10.1687
1193
PRIMER_LEFT_3_PENALTY=4.584153
1194
PRIMER_RIGHT_3_PENALTY=5.584511
1195
PRIMER_LEFT_3_SEQUENCE=ATGGCGCTGAAAAGAATCCAC
1196
PRIMER_RIGHT_3_SEQUENCE=TTACATCGCATACTTCTGAGTCC
1197
PRIMER_LEFT_3=0,21
1198
PRIMER_RIGHT_3=443,23
1199
PRIMER_LEFT_3_TM=63.584
1200
PRIMER_RIGHT_3_TM=58.415
1201
PRIMER_LEFT_3_GC_PERCENT=47.619
1202
PRIMER_RIGHT_3_GC_PERCENT=43.478
1203
PRIMER_LEFT_3_SELF_ANY=4.00
1204
PRIMER_RIGHT_3_SELF_ANY=3.00
1205
PRIMER_LEFT_3_SELF_END=0.00
1206
PRIMER_RIGHT_3_SELF_END=1.00
1207
PRIMER_LEFT_3_POSITION_PENALTY=0.000000
1208
PRIMER_RIGHT_3_POSITION_PENALTY=1.000000
1209
PRIMER_LEFT_3_END_STABILITY=7.9000
1210
PRIMER_RIGHT_3_END_STABILITY=7.6000
1211
PRIMER_PAIR_3_COMPL_ANY=5.00
1212
PRIMER_PAIR_3_COMPL_END=2.00
1213
PRIMER_PRODUCT_SIZE_3=444
1214
PRIMER_PAIR_PENALTY_4=10.6050
1215
PRIMER_LEFT_4_PENALTY=4.584153
1216
PRIMER_RIGHT_4_PENALTY=6.020829
1217
PRIMER_LEFT_4_SEQUENCE=ATGGCGCTGAAAAGAATCCAC
1218
PRIMER_RIGHT_4_SEQUENCE=TTACATCGCATACTTCTGAGTCCA
1219
PRIMER_LEFT_4=0,21
1220
PRIMER_RIGHT_4=443,24
1221
PRIMER_LEFT_4_TM=63.584
1222
PRIMER_RIGHT_4_TM=61.021
1223
PRIMER_LEFT_4_GC_PERCENT=47.619
1224
PRIMER_RIGHT_4_GC_PERCENT=41.667
1225
PRIMER_LEFT_4_SELF_ANY=4.00
1226
PRIMER_RIGHT_4_SELF_ANY=3.00
1227
PRIMER_LEFT_4_SELF_END=0.00
1228
PRIMER_RIGHT_4_SELF_END=1.00
1229
PRIMER_LEFT_4_POSITION_PENALTY=0.000000
1230
PRIMER_RIGHT_4_POSITION_PENALTY=1.000000
1231
PRIMER_LEFT_4_END_STABILITY=7.9000
1232
PRIMER_RIGHT_4_END_STABILITY=7.9000
1233
PRIMER_PAIR_4_COMPL_ANY=5.00
1234
PRIMER_PAIR_4_COMPL_END=3.00
1235
PRIMER_PRODUCT_SIZE_4=444
1236
PRIMER_PAIR_PENALTY_5=63.2969
1237
PRIMER_LEFT_5_PENALTY=61.060283
1238
PRIMER_RIGHT_5_PENALTY=2.236656
1239
PRIMER_LEFT_5_SEQUENCE=GCGCTGAAAAGAATCCACA
1240
PRIMER_RIGHT_5_SEQUENCE=ACATCGCATACTTCTGAGTCCA
1241
PRIMER_LEFT_5=3,19
1242
PRIMER_RIGHT_5=441,22
1243
PRIMER_LEFT_5_TM=59.940
1244
PRIMER_RIGHT_5_TM=59.763
1245
PRIMER_LEFT_5_GC_PERCENT=47.368
1246
PRIMER_RIGHT_5_GC_PERCENT=45.455
1247
PRIMER_LEFT_5_SELF_ANY=4.00
1248
PRIMER_RIGHT_5_SELF_ANY=3.00
1249
PRIMER_LEFT_5_SELF_END=0.00
1250
PRIMER_RIGHT_5_SELF_END=1.00
1251
PRIMER_LEFT_5_POSITION_PENALTY=60.000000
1252
PRIMER_RIGHT_5_POSITION_PENALTY=0.000000
1253
PRIMER_LEFT_5_END_STABILITY=8.2000
1254
PRIMER_RIGHT_5_END_STABILITY=7.9000
1255
PRIMER_PAIR_5_COMPL_ANY=5.00
1256
PRIMER_PAIR_5_COMPL_END=2.00
1257
PRIMER_PRODUCT_SIZE_5=439
1258
PRIMER_PAIR_PENALTY_6=63.5215
1259
PRIMER_LEFT_6_PENALTY=61.284892
1260
PRIMER_RIGHT_6_PENALTY=2.236656
1261
PRIMER_LEFT_6_SEQUENCE=GCGCTGAAAAGAATCCACAA
1262
PRIMER_RIGHT_6_SEQUENCE=ACATCGCATACTTCTGAGTCCA
1263
PRIMER_LEFT_6=3,20
1264
PRIMER_RIGHT_6=441,22
1265
PRIMER_LEFT_6_TM=61.285
1266
PRIMER_RIGHT_6_TM=59.763
1267
PRIMER_LEFT_6_GC_PERCENT=45.000
1268
PRIMER_RIGHT_6_GC_PERCENT=45.455
1269
PRIMER_LEFT_6_SELF_ANY=4.00
1270
PRIMER_RIGHT_6_SELF_ANY=3.00
1271
PRIMER_LEFT_6_SELF_END=0.00
1272
PRIMER_RIGHT_6_SELF_END=1.00
1273
PRIMER_LEFT_6_POSITION_PENALTY=60.000000
1274
PRIMER_RIGHT_6_POSITION_PENALTY=0.000000
1275
PRIMER_LEFT_6_END_STABILITY=7.0000
1276
PRIMER_RIGHT_6_END_STABILITY=7.9000
1277
PRIMER_PAIR_6_COMPL_ANY=5.00
1278
PRIMER_PAIR_6_COMPL_END=1.00
1279
PRIMER_PRODUCT_SIZE_6=439
1280
PRIMER_PAIR_PENALTY_7=64.0998
1281
PRIMER_LEFT_7_PENALTY=61.060283
1282
PRIMER_RIGHT_7_PENALTY=3.039531
1283
PRIMER_LEFT_7_SEQUENCE=GCGCTGAAAAGAATCCACA
1284
PRIMER_RIGHT_7_SEQUENCE=ACATCGCATACTTCTGAGTCCAT
1285
PRIMER_LEFT_7=3,19
1286
PRIMER_RIGHT_7=441,23
1287
PRIMER_LEFT_7_TM=59.940
1288
PRIMER_RIGHT_7_TM=60.040
1289
PRIMER_LEFT_7_GC_PERCENT=47.368
1290
PRIMER_RIGHT_7_GC_PERCENT=43.478
1291
PRIMER_LEFT_7_SELF_ANY=4.00
1292
PRIMER_RIGHT_7_SELF_ANY=3.00
1293
PRIMER_LEFT_7_SELF_END=0.00
1294
PRIMER_RIGHT_7_SELF_END=2.00
1295
PRIMER_LEFT_7_POSITION_PENALTY=60.000000
1296
PRIMER_RIGHT_7_POSITION_PENALTY=0.000000
1297
PRIMER_LEFT_7_END_STABILITY=8.2000
1298
PRIMER_RIGHT_7_END_STABILITY=8.1000
1299
PRIMER_PAIR_7_COMPL_ANY=5.00
1300
PRIMER_PAIR_7_COMPL_END=2.00
1301
PRIMER_PRODUCT_SIZE_7=439
1302
PRIMER_PAIR_PENALTY_8=64.3244
1303
PRIMER_LEFT_8_PENALTY=61.284892
1304
PRIMER_RIGHT_8_PENALTY=3.039531
1305
PRIMER_LEFT_8_SEQUENCE=GCGCTGAAAAGAATCCACAA
1306
PRIMER_RIGHT_8_SEQUENCE=ACATCGCATACTTCTGAGTCCAT
1307
PRIMER_LEFT_8=3,20
1308
PRIMER_RIGHT_8=441,23
1309
PRIMER_LEFT_8_TM=61.285
1310
PRIMER_RIGHT_8_TM=60.040
1311
PRIMER_LEFT_8_GC_PERCENT=45.000
1312
PRIMER_RIGHT_8_GC_PERCENT=43.478
1313
PRIMER_LEFT_8_SELF_ANY=4.00
1314
PRIMER_RIGHT_8_SELF_ANY=3.00
1315
PRIMER_LEFT_8_SELF_END=0.00
1316
PRIMER_RIGHT_8_SELF_END=2.00
1317
PRIMER_LEFT_8_POSITION_PENALTY=60.000000
1318
PRIMER_RIGHT_8_POSITION_PENALTY=0.000000
1319
PRIMER_LEFT_8_END_STABILITY=7.0000
1320
PRIMER_RIGHT_8_END_STABILITY=8.1000
1321
PRIMER_PAIR_8_COMPL_ANY=5.00
1322
PRIMER_PAIR_8_COMPL_END=2.00
1323
PRIMER_PRODUCT_SIZE_8=439
1324
PRIMER_PAIR_PENALTY_9=64.7776
1325
PRIMER_LEFT_9_PENALTY=61.060283
1326
PRIMER_RIGHT_9_PENALTY=3.717312
1327
PRIMER_LEFT_9_SEQUENCE=GCGCTGAAAAGAATCCACA
1328
PRIMER_RIGHT_9_SEQUENCE=TACATCGCATACTTCTGAGTCCA
1329
PRIMER_LEFT_9=3,19
1330
PRIMER_RIGHT_9=442,23
1331
PRIMER_LEFT_9_TM=59.940
1332
PRIMER_RIGHT_9_TM=59.783
1333
PRIMER_LEFT_9_GC_PERCENT=47.368
1334
PRIMER_RIGHT_9_GC_PERCENT=43.478
1335
PRIMER_LEFT_9_SELF_ANY=4.00
1336
PRIMER_RIGHT_9_SELF_ANY=3.00
1337
PRIMER_LEFT_9_SELF_END=0.00
1338
PRIMER_RIGHT_9_SELF_END=1.00
1339
PRIMER_LEFT_9_POSITION_PENALTY=60.000000
1340
PRIMER_RIGHT_9_POSITION_PENALTY=0.500000
1341
PRIMER_LEFT_9_END_STABILITY=8.2000
1342
PRIMER_RIGHT_9_END_STABILITY=7.9000
1343
PRIMER_PAIR_9_COMPL_ANY=5.00
1344
PRIMER_PAIR_9_COMPL_END=2.00
1345
PRIMER_PRODUCT_SIZE_9=440
1346
PRIMER_PAIR_PENALTY_10=65.0022
1347
PRIMER_LEFT_10_PENALTY=61.284892
1348
PRIMER_RIGHT_10_PENALTY=3.717312
1349
PRIMER_LEFT_10_SEQUENCE=GCGCTGAAAAGAATCCACAA
1350
PRIMER_RIGHT_10_SEQUENCE=TACATCGCATACTTCTGAGTCCA
1351
PRIMER_LEFT_10=3,20
1352
PRIMER_RIGHT_10=442,23
1353
PRIMER_LEFT_10_TM=61.285
1354
PRIMER_RIGHT_10_TM=59.783
1355
PRIMER_LEFT_10_GC_PERCENT=45.000
1356
PRIMER_RIGHT_10_GC_PERCENT=43.478
1357
PRIMER_LEFT_10_SELF_ANY=4.00
1358
PRIMER_RIGHT_10_SELF_ANY=3.00
1359
PRIMER_LEFT_10_SELF_END=0.00
1360
PRIMER_RIGHT_10_SELF_END=1.00
1361
PRIMER_LEFT_10_POSITION_PENALTY=60.000000
1362
PRIMER_RIGHT_10_POSITION_PENALTY=0.500000
1363
PRIMER_LEFT_10_END_STABILITY=7.0000
1364
PRIMER_RIGHT_10_END_STABILITY=7.9000
1365
PRIMER_PAIR_10_COMPL_ANY=5.00
1366
PRIMER_PAIR_10_COMPL_END=1.00
1367
PRIMER_PRODUCT_SIZE_10=440
1368
PRIMER_PAIR_PENALTY_11=65.3838
1369
PRIMER_LEFT_11_PENALTY=63.147152
1370
PRIMER_RIGHT_11_PENALTY=2.236656
1371
PRIMER_LEFT_11_SEQUENCE=GCGCTGAAAAGAATCCACAAG
1372
PRIMER_RIGHT_11_SEQUENCE=ACATCGCATACTTCTGAGTCCA
1373
PRIMER_LEFT_11=3,21
1374
PRIMER_RIGHT_11=441,22
1375
PRIMER_LEFT_11_TM=62.147
1376
PRIMER_RIGHT_11_TM=59.763
1377
PRIMER_LEFT_11_GC_PERCENT=47.619
1378
PRIMER_RIGHT_11_GC_PERCENT=45.455
1379
PRIMER_LEFT_11_SELF_ANY=4.00
1380
PRIMER_RIGHT_11_SELF_ANY=3.00
1381
PRIMER_LEFT_11_SELF_END=0.00
1382
PRIMER_RIGHT_11_SELF_END=1.00
1383
PRIMER_LEFT_11_POSITION_PENALTY=60.000000
1384
PRIMER_RIGHT_11_POSITION_PENALTY=0.000000
1385
PRIMER_LEFT_11_END_STABILITY=6.7000
1386
PRIMER_RIGHT_11_END_STABILITY=7.9000
1387
PRIMER_PAIR_11_COMPL_ANY=5.00
1388
PRIMER_PAIR_11_COMPL_END=2.00
1389
PRIMER_PRODUCT_SIZE_11=439
1390
PRIMER_PAIR_PENALTY_12=65.6084
1391
PRIMER_LEFT_12_PENALTY=61.060283
1392
PRIMER_RIGHT_12_PENALTY=4.548131
1393
PRIMER_LEFT_12_SEQUENCE=GCGCTGAAAAGAATCCACA
1394
PRIMER_RIGHT_12_SEQUENCE=TACATCGCATACTTCTGAGTCCAT
1395
PRIMER_LEFT_12=3,19
1396
PRIMER_RIGHT_12=442,24
1397
PRIMER_LEFT_12_TM=59.940
1398
PRIMER_RIGHT_12_TM=60.048
1399
PRIMER_LEFT_12_GC_PERCENT=47.368
1400
PRIMER_RIGHT_12_GC_PERCENT=41.667
1401
PRIMER_LEFT_12_SELF_ANY=4.00
1402
PRIMER_RIGHT_12_SELF_ANY=3.00
1403
PRIMER_LEFT_12_SELF_END=0.00
1404
PRIMER_RIGHT_12_SELF_END=2.00
1405
PRIMER_LEFT_12_POSITION_PENALTY=60.000000
1406
PRIMER_RIGHT_12_POSITION_PENALTY=0.500000
1407
PRIMER_LEFT_12_END_STABILITY=8.2000
1408
PRIMER_RIGHT_12_END_STABILITY=8.1000
1409
PRIMER_PAIR_12_COMPL_ANY=5.00
1410
PRIMER_PAIR_12_COMPL_END=2.00
1411
PRIMER_PRODUCT_SIZE_12=440
1412
PRIMER_PAIR_PENALTY_13=65.8330
1413
PRIMER_LEFT_13_PENALTY=61.284892
1414
PRIMER_RIGHT_13_PENALTY=4.548131
1415
PRIMER_LEFT_13_SEQUENCE=GCGCTGAAAAGAATCCACAA
1416
PRIMER_RIGHT_13_SEQUENCE=TACATCGCATACTTCTGAGTCCAT
1417
PRIMER_LEFT_13=3,20
1418
PRIMER_RIGHT_13=442,24
1419
PRIMER_LEFT_13_TM=61.285
1420
PRIMER_RIGHT_13_TM=60.048
1421
PRIMER_LEFT_13_GC_PERCENT=45.000
1422
PRIMER_RIGHT_13_GC_PERCENT=41.667
1423
PRIMER_LEFT_13_SELF_ANY=4.00
1424
PRIMER_RIGHT_13_SELF_ANY=3.00
1425
PRIMER_LEFT_13_SELF_END=0.00
1426
PRIMER_RIGHT_13_SELF_END=2.00
1427
PRIMER_LEFT_13_POSITION_PENALTY=60.000000
1428
PRIMER_RIGHT_13_POSITION_PENALTY=0.500000
1429
PRIMER_LEFT_13_END_STABILITY=7.0000
1430
PRIMER_RIGHT_13_END_STABILITY=8.1000
1431
PRIMER_PAIR_13_COMPL_ANY=5.00
1432
PRIMER_PAIR_13_COMPL_END=2.00
1433
PRIMER_PRODUCT_SIZE_13=440
1434
PRIMER_PAIR_PENALTY_14=66.1867
1435
PRIMER_LEFT_14_PENALTY=63.147152
1436
PRIMER_RIGHT_14_PENALTY=3.039531
1437
PRIMER_LEFT_14_SEQUENCE=GCGCTGAAAAGAATCCACAAG
1438
PRIMER_RIGHT_14_SEQUENCE=ACATCGCATACTTCTGAGTCCAT
1439
PRIMER_LEFT_14=3,21
1440
PRIMER_RIGHT_14=441,23
1441
PRIMER_LEFT_14_TM=62.147
1442
PRIMER_RIGHT_14_TM=60.040
1443
PRIMER_LEFT_14_GC_PERCENT=47.619
1444
PRIMER_RIGHT_14_GC_PERCENT=43.478
1445
PRIMER_LEFT_14_SELF_ANY=4.00
1446
PRIMER_RIGHT_14_SELF_ANY=3.00
1447
PRIMER_LEFT_14_SELF_END=0.00
1448
PRIMER_RIGHT_14_SELF_END=2.00
1449
PRIMER_LEFT_14_POSITION_PENALTY=60.000000
1450
PRIMER_RIGHT_14_POSITION_PENALTY=0.000000
1451
PRIMER_LEFT_14_END_STABILITY=6.7000
1452
PRIMER_RIGHT_14_END_STABILITY=8.1000
1453
PRIMER_PAIR_14_COMPL_ANY=5.00
1454
PRIMER_PAIR_14_COMPL_END=3.00
1455
PRIMER_PRODUCT_SIZE_14=439
1456
PRIMER_PAIR_PENALTY_15=66.3073
1457
PRIMER_LEFT_15_PENALTY=61.060283
1458
PRIMER_RIGHT_15_PENALTY=5.247065
1459
PRIMER_LEFT_15_SEQUENCE=GCGCTGAAAAGAATCCACA
1460
PRIMER_RIGHT_15_SEQUENCE=ACATCGCATACTTCTGAGTCCATT
1461
PRIMER_LEFT_15=3,19
1462
PRIMER_RIGHT_15=441,24
1463
PRIMER_LEFT_15_TM=59.940
1464
PRIMER_RIGHT_15_TM=61.247
1465
PRIMER_LEFT_15_GC_PERCENT=47.368
1466
PRIMER_RIGHT_15_GC_PERCENT=41.667
1467
PRIMER_LEFT_15_SELF_ANY=4.00
1468
PRIMER_RIGHT_15_SELF_ANY=3.00
1469
PRIMER_LEFT_15_SELF_END=0.00
1470
PRIMER_RIGHT_15_SELF_END=1.00
1471
PRIMER_LEFT_15_POSITION_PENALTY=60.000000
1472
PRIMER_RIGHT_15_POSITION_PENALTY=0.000000
1473
PRIMER_LEFT_15_END_STABILITY=8.2000
1474
PRIMER_RIGHT_15_END_STABILITY=8.4000
1475
PRIMER_PAIR_15_COMPL_ANY=5.00
1476
PRIMER_PAIR_15_COMPL_END=2.00
1477
PRIMER_PRODUCT_SIZE_15=439
1478
PRIMER_PAIR_PENALTY_16=66.5320
1479
PRIMER_LEFT_16_PENALTY=61.284892
1480
PRIMER_RIGHT_16_PENALTY=5.247065
1481
PRIMER_LEFT_16_SEQUENCE=GCGCTGAAAAGAATCCACAA
1482
PRIMER_RIGHT_16_SEQUENCE=ACATCGCATACTTCTGAGTCCATT
1483
PRIMER_LEFT_16=3,20
1484
PRIMER_RIGHT_16=441,24
1485
PRIMER_LEFT_16_TM=61.285
1486
PRIMER_RIGHT_16_TM=61.247
1487
PRIMER_LEFT_16_GC_PERCENT=45.000
1488
PRIMER_RIGHT_16_GC_PERCENT=41.667
1489
PRIMER_LEFT_16_SELF_ANY=4.00
1490
PRIMER_RIGHT_16_SELF_ANY=3.00
1491
PRIMER_LEFT_16_SELF_END=0.00
1492
PRIMER_RIGHT_16_SELF_END=1.00
1493
PRIMER_LEFT_16_POSITION_PENALTY=60.000000
1494
PRIMER_RIGHT_16_POSITION_PENALTY=0.000000
1495
PRIMER_LEFT_16_END_STABILITY=7.0000
1496
PRIMER_RIGHT_16_END_STABILITY=8.4000
1497
PRIMER_PAIR_16_COMPL_ANY=5.00
1498
PRIMER_PAIR_16_COMPL_END=3.00
1499
PRIMER_PRODUCT_SIZE_16=439
1500
PRIMER_PAIR_PENALTY_17=66.6448
1501
PRIMER_LEFT_17_PENALTY=61.060283
1502
PRIMER_RIGHT_17_PENALTY=5.584511
1503
PRIMER_LEFT_17_SEQUENCE=GCGCTGAAAAGAATCCACA
1504
PRIMER_RIGHT_17_SEQUENCE=TTACATCGCATACTTCTGAGTCC
1505
PRIMER_LEFT_17=3,19
1506
PRIMER_RIGHT_17=443,23
1507
PRIMER_LEFT_17_TM=59.940
1508
PRIMER_RIGHT_17_TM=58.415
1509
PRIMER_LEFT_17_GC_PERCENT=47.368
1510
PRIMER_RIGHT_17_GC_PERCENT=43.478
1511
PRIMER_LEFT_17_SELF_ANY=4.00
1512
PRIMER_RIGHT_17_SELF_ANY=3.00
1513
PRIMER_LEFT_17_SELF_END=0.00
1514
PRIMER_RIGHT_17_SELF_END=1.00
1515
PRIMER_LEFT_17_POSITION_PENALTY=60.000000
1516
PRIMER_RIGHT_17_POSITION_PENALTY=1.000000
1517
PRIMER_LEFT_17_END_STABILITY=8.2000
1518
PRIMER_RIGHT_17_END_STABILITY=7.6000
1519
PRIMER_PAIR_17_COMPL_ANY=5.00
1520
PRIMER_PAIR_17_COMPL_END=2.00
1521
PRIMER_PRODUCT_SIZE_17=441
1522
PRIMER_PAIR_PENALTY_18=66.8645
1523
PRIMER_LEFT_18_PENALTY=63.147152
1524
PRIMER_RIGHT_18_PENALTY=3.717312
1525
PRIMER_LEFT_18_SEQUENCE=GCGCTGAAAAGAATCCACAAG
1526
PRIMER_RIGHT_18_SEQUENCE=TACATCGCATACTTCTGAGTCCA
1527
PRIMER_LEFT_18=3,21
1528
PRIMER_RIGHT_18=442,23
1529
PRIMER_LEFT_18_TM=62.147
1530
PRIMER_RIGHT_18_TM=59.783
1531
PRIMER_LEFT_18_GC_PERCENT=47.619
1532
PRIMER_RIGHT_18_GC_PERCENT=43.478
1533
PRIMER_LEFT_18_SELF_ANY=4.00
1534
PRIMER_RIGHT_18_SELF_ANY=3.00
1535
PRIMER_LEFT_18_SELF_END=0.00
1536
PRIMER_RIGHT_18_SELF_END=1.00
1537
PRIMER_LEFT_18_POSITION_PENALTY=60.000000
1538
PRIMER_RIGHT_18_POSITION_PENALTY=0.500000
1539
PRIMER_LEFT_18_END_STABILITY=6.7000
1540
PRIMER_RIGHT_18_END_STABILITY=7.9000
1541
PRIMER_PAIR_18_COMPL_ANY=5.00
1542
PRIMER_PAIR_18_COMPL_END=2.00
1543
PRIMER_PRODUCT_SIZE_18=440
1544
PRIMER_PAIR_PENALTY_19=66.8694
1545
PRIMER_LEFT_19_PENALTY=61.284892
1546
PRIMER_RIGHT_19_PENALTY=5.584511
1547
PRIMER_LEFT_19_SEQUENCE=GCGCTGAAAAGAATCCACAA
1548
PRIMER_RIGHT_19_SEQUENCE=TTACATCGCATACTTCTGAGTCC
1549
PRIMER_LEFT_19=3,20
1550
PRIMER_RIGHT_19=443,23
1551
PRIMER_LEFT_19_TM=61.285
1552
PRIMER_RIGHT_19_TM=58.415
1553
PRIMER_LEFT_19_GC_PERCENT=45.000
1554
PRIMER_RIGHT_19_GC_PERCENT=43.478
1555
PRIMER_LEFT_19_SELF_ANY=4.00
1556
PRIMER_RIGHT_19_SELF_ANY=3.00
1557
PRIMER_LEFT_19_SELF_END=0.00
1558
PRIMER_RIGHT_19_SELF_END=1.00
1559
PRIMER_LEFT_19_POSITION_PENALTY=60.000000
1560
PRIMER_RIGHT_19_POSITION_PENALTY=1.000000
1561
PRIMER_LEFT_19_END_STABILITY=7.0000
1562
PRIMER_RIGHT_19_END_STABILITY=7.6000
1563
PRIMER_PAIR_19_COMPL_ANY=5.00
1564
PRIMER_PAIR_19_COMPL_END=2.00
1565
PRIMER_PRODUCT_SIZE_19=441
1566
=
1567
PRIMER_SEQUENCE_ID=U51903/IQGAP2
1568
PRIMER_START_CODON_POSITION=222
1569
PRIMER_LEFT_INPUT=ATGCCACACGAAGAGCTGC
1570
SEQUENCE=GAGGGAGGAGAGTTCACTTTTACTTCAGTGTCAGCGCGCGGCGGCCGTGGCTGGCTCTGGCGAGAGAGCACCGAGGGAGTGGGTCGCAGATCTTCGGGCGGCTAGGGGAAATCGGCGAGAGGCGGGATCCGAGCGCGCCGGCGGGGCGCAGAGCCCGCGAGCCTGGCCAGCGAGGGTAGCCGCGGGGGGCGCGCCCCGGGCGGGCCCCCGGAGACGCGCAGGATGCCACACGAAGAGCTGCCGTCGCTGCAGAGACCCCGCTATGGCTCTATTGTGGACGATGAAAGGCTCTCTGCAGAGGAGATGGATGAGAGGAGGCGGCAGAACATTGCTTATGAATATCTGTGCCACTTAGAGGAAGCCAAAAGGTGGATGGAAGTTTGCTTAGTTGAAGAATTGCCACCAACCACTGAATTGGAAGAAGGGCTCCGGAATGGAGTTTACCTTGCAAAGTTAGCCAAGTTCTTTGCCCCGAAAATGGTATCAGAGAAAAAGATCTATGATGTGGAACAAACACGTTATAAGAAGTCTGGCCTTCATTTTCGACACACAGATAATACCGTCCAGTGGTTAAGAGCGATGGAGTCTATTGGTCTACCCAAGATATTTTATCCAGAAACAACAGATGTCTATGATCGGAAAAACATACCAAGAATGATATATTGCATTCACGCACTGAGTTTGTATCTGTTCAAACTAGGAATAGCACCCCAGATCCAGGATTTGTTGGGCAAAGTAGACTTCACAGAGGAGGAAATCAGTAATATGAGAAAAGAACTTGAGAAATATGGAATACAGATGCCATCTTTCAGCAAAATAGGTGGTATTCTGGCCAATGAACTGTCCGTGGATGAAGCTGCATTACATGCTGCAGTTATAGCCATTAATGAAGCAGTTGAAAAAGGAATAGCAGAGCAAACCGTTGTAACACTAAGAAACCCAAATGCGGTTTTAACTTTAGTGGATGACAACCTTGCACCAGAATATCAGAAAGAACTCTGGGATGCCAAAAAGAAAAAAGAGGAAAATGCAAGACTGAAGAATAGCTGTATTTCAGAAGAAGAAAGAGATGCTTATGAAGAACTGCTGACACAAGCAGAAATCCAAGGCAATATTAATAAAGTCAACAGGCAGGCTGCAGTGGACCATATCAATGCTGTCATTCCGGAAGGTGACCCCGAGAATACGCTGCTTGCACTGAAGAAACCAGAGGCCCAGCTGCCTGCTGTTTATCCCTTTGCTGCTGCCATGTATCAGAACGAACTTTTCAACCTCCAGAAACAGAACACCATGAACTACTTGGCCCACGAGGAGCTTTTGATTGCTGTGGAAATGTTGTCTGCTGTTGCTTTACTAAACCAGGCCTTGGAAAGCAACGATCTTGTGTCTGTGCAGAATCAACTCAGAAGCCCCGCAATAGGCTTAAACAATCTGGACAAGGCATATGTGGAACGTTATGCAAACACACTACTCTCTGTTAAACTAGAAGTTTTATCCCAAGGGCAAGATAACTTAAGCTGGAATGAAATTCAGAATTGTATTGATATGGTTAATGCTCAAATTCAAGAAGAAAATGACCGAGTTGTAGCTGTAGGGTACATCAATGAAGCTATTGATGAAGGGAATCCTTTGAGGACTTTAGAAACTTTGCTCCTACCTACTGCGAATATTAGTGATGTGGACCCAGCCCATGCCCAGCACTACCAGGATGTTTTATACCATGCTAAATCACAGAAACTCGGAGACTCTGAGAGTGTTTCCAAAGTGCTTTGGCTGGATGAGATACAGCAAGCCGTCGATGAGGCCAACGTGGACGAGGACAGAGCAAAACAATGGGTTACTCTGGTGGTTGATGTTAATCAGTGTTTGGAAGGAAAAAAATCAAGTGATATTTTGTCTGTATTGAAGTCTTCCACTTCTAATGCAAATGACATAATCCCGGAGTGTGCTGACAAATACTATGATGCCCTTGTGAAGGCAAAAGAGCTCAAATCTGAAAGAGTGTCTAGTGACGGTTCATGGCTCAAACTCAACCTGCACAAAAAATATGACTACTATTACAACACTGATTCAAAAGAGAGTTCCTGGGTCACACCTGAATCATGCTTCTATAAAGAATCATGGCTCACAGGAAAAGAAATCGAGGACATTATTGAGGAAGTCACAGTAGGTTACATTCGTGAGAATATATGGTCTGCTTCAGAAGAGTTGCTTCTTCGCTTTCAAGCCACAAGCTCAGGACCCATCCTTAGGGAAGAGTTTGAAGCTAGAAAATCATTTTTGCATGAACAAGAAGAGAATGTGGTCAAAATACAGGCTTTTTGGAAAGGATATAAACAACGGAAGGAGTATATGCACAGGCGGCAAACGTTCATTGATAATACTGATTCTGTTGTGAAGATTCAGTCCTGGTTCCGAATGGCAACTGCAAGAAAGAGCTATCTTTCAAGACTACAGTATTTCAGAGATCATAATAATGAAATTGTGAAAATACAGTCACTGTTGAGAGCGAACAAAGCTAGAGATGACTACAAAACATTGGTTGGCTCTGAAAACCCACCATTAACAGTAATTCGCAAATTTGTATACCTGCTGGACCAAAGTGATTTGGATTTCCAGGAGGAACTAGAGGTTGCACGATTAAGGGAAGAAGTAGTGACCAAGATCAGGGCCAATCAACAGCTGGAAAAAGACCTGAACCTGATGGACATCAAGATTGGACTGCTGGTGAAGAACAGGATCACACTAGAGGATGTAATTTCACACAGTAAAAAGCTGAACAAGAAAAAAGGAGGAGAAATGGAAATACTGAATAACACCGACAACCAAGGAATAAAAAGTTTGAGTAAGGAGAGGAGAAAAACACTAGAAACATATCAGCAGCTGTTTTACCTTTTACAGACCAACCCTTTATACTTGGCTAAGCTGATTTTCCAGATGCCACAGAACAAGTCCACTAAATTTATGGATACTGTTATTTTCACACTATATAATTATGCCTCTAATCAGCGAGAAGAATATCTACTTCTCAAGCTTTTTAAAACTGCTCTGGAGGAAGAAATAAAATCAAAAGTGGACCAGGTACAGGACATAGTTACTGGTAACCCTACAGTCATCAAGATGGTCGTCAGCTTCAATAGAGGTGCCCGGGGACAGAACACCCTGCGCCAACTCCTGGCTCCAGTGGTAAAAGAGATCATCGACGACAAGTCGCTGATTATCAACACAAACCCTGTAGAGGTGTACAAGGCTTGGGTGAACCAACTAGAAACACAGACTGGAGAGGCCAGCAAGTTGCCTTATGATGTGACCACAGAACAAGCTCTAACATACCCAGAAGTGAAAAATAAACTGGAGGCTTCCATTGAGAACCTGAGAAGGGTCACCGACAAAGTCCTGAATTCTATCATTTCTTCCCTTGATCTACTGCCTTATGGATTGAGGTATATAGCCAAAGTACTGAAGAATTCGATCCATGAGAAATTCCCCGATGCAACAGAAGATGAGCTATTAAAGATTGTTGGAAACCTCCTGTACTATCGGTACATGAATCCAGCCATTGTAGCTCCAGATGGCTTTGATATCATCGACATGACAGCTGGAGGTCAGATAAATTCTGACCAAAGGAGAAACTTAGGATCAGTGGCCAAGGTTCTTCAGCACGCAGCCTCCAACAAGCTGTTTGAAGGAGAAAATGAGCATCTCTCATCTATGAACAATTATTTATCAGAGACGTATCAGGAATTCAGGAAATATTTCAAAGAAGCATGTAATGTCCCTGAGCCAGAAGAGAAGTTTAATATGGACAAATACACAGACCTGGTGACAGTCAGCAAACCAGTCATTTATATTTCAATTGAAGAAATCATCAGCACACACTCACTCCTGTTGGAACACCAGGATGCAATTGCCCCTGAGAAAAATGACTTACTGAGTGAATTGCTGGGGTCGCTGGGAGAGGTGCCAACCGTGGAATCTTTTCTTGGGGAAGGAGCAGTTGACCCCAATGACCCTAACAAGGCAAATACACTAAGTCAGCTTTCAAAGACCGAGATTTCTCTTGTCTTGACAAGCAAATATGACATAGAGGACGGTGAAGCTATAGATAGCCGAAGCCTCATGATAAAGACCAAGAAGCTGATAATTGATGTGATCCGGAACCAGCCAGGGAACACATTGACAGAAATCTTAGAGACACCAGCAACTGCGCAACAGGAGGTAGACCATGCCACGGACATGGTGAGCCGTGCAATGATAGATTCCAGGACTCCAGAAGAAATGAAGCATAGCCAATCTATGATTGAAGATGCACAGCTGCCTCTTGAGCAGAAGAAGAGGAAAATCCAGAGGAATCTTCGGACGTTGGAACAGACTGGACACGTGTCATCCGAAAATAAATACCAAGACATTCTCAATGAGATTGCCAAGGATATTCGAAATCAAAGAATCTATCGTAAGCTTCGAAAAGCTGAATTGGCAAAACTTCAGCAGACCCTGAATGCACTTAACAAGAAGGCAGCATTTTATGAAGAGCAAATCAATTATTATGACACCTACATAAAGACTTGTTTAGACAACTTAAAAAGAAAAAATACTCGGAGATCAATTAAACTAGATGGAAAAGGAGAACCCAAAGGGGCGAAGAGAGCGAAGCCAGTGAAGTACACTGCAGCAAAGCTGCATGAGAAAGGTGTCCTGCTAGATATAGATGATCTTCAAACAAACCAGTTTAAGAATGTTACATTTGATATCATAGCTACTGAAGATGTAGGCATTTTCGATGTAAGATCAAAATTCCTTGGTGTTGAGATGGAAAAGGTGCAACTCAATATTCAGGATTTACTTCAGATGCAATATGAAGGAGTAGCTGTAATGAAAATGTTTGATAAGGTTAAAGTGAATGTAAACCTTCTCATATACCTGCTGAACAAGAAGTTCTATGGAAAGTGAAGTGCCTACAGAAATTTCTTGGATTCTGTATCATCTGGATTAGGAAATGAATTTGTTTAATATTTTTGTTTTTAAACATGATTGAAATCACTGCTTATAAATGTGTGATTTTTTTTAAATGACCAAAACTGTTCTGAAGAATGTACCCAGGTGCCTTTTTGCTAATTTGATACTATAATAGAATGAGACATAAAATGAATTAATGGAAACATATCCACACTGTACTGTGATATAGGTACTCTGATTTAAAACTTTGGACATCCTGTGATCTGTTTTAAAGTTGGGGGGTGGGAAATTTAGCTGACTAGGGACAAACATGTAAACCTATTTTCCTATGAAAAAAGTTTTAAATGTCCCACTTGAATAACGTAATTCTTCATAGTTTTTTTAATCTATGGATAAATGGAAACCTAATTATTTGTAATGAATTATTTAGACAGTTCTAAGCCCTGTCTTCTGGGAGTTATCAATTTTAAAGAGAACTTTTGTGCAATTCAAATGAAGTTTTTATAAGTAATTGAAAATGACAACACAATAACACTTTCTGTATAAAAGTATATATTTTATGTGATTTATTCCTACTAAATGAAAGTGCACTACTGCCTCATGTAAAGACTCTTGCACGCAGAGCCTTTAAGTGACTAAGGAACAACATAGATAGTGAGCATAGTCCCCACCTCCACCCCTCACAATTTATTTGAATACTTCAATTGTGCCTCTCAATTTTTTGTAATGCTAAAAAATCAGTATCTAGATGGTTTTTAAATGTATTCTCTGGAAATTGTTTTATGTAAAATAAATGTTACTTAATTCCATT
1571
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
1572
PRIMER_RIGHT_EXPLAIN=considered 49099, GC content failed 2042, low tm 18100, high tm 10315, high end compl 16, long poly-x seq 226,high 3' stability 109, ok 18291
1573
PRIMER_PAIR_EXPLAIN=considered 20, ok 20
1574
PRIMER_STOP_CODON_POSITION=4947
1575
PRIMER_PAIR_PENALTY=10.1097
1576
PRIMER_LEFT_PENALTY=3.573461
1577
PRIMER_RIGHT_PENALTY=6.536286
1578
PRIMER_LEFT_SEQUENCE=ATGCCACACGAAGAGCTGC
1579
PRIMER_RIGHT_SEQUENCE=ACTTTCCATAGAACTTCTTGTTCAGC
1580
PRIMER_LEFT=222,19
1581
PRIMER_RIGHT=4947,26
1582
PRIMER_LEFT_TM=62.573
1583
PRIMER_RIGHT_TM=60.536
1584
PRIMER_LEFT_GC_PERCENT=57.895
1585
PRIMER_RIGHT_GC_PERCENT=38.462
1586
PRIMER_LEFT_SELF_ANY=4.00
1587
PRIMER_RIGHT_SELF_ANY=7.00
1588
PRIMER_LEFT_SELF_END=3.00
1589
PRIMER_RIGHT_SELF_END=3.00
1590
PRIMER_LEFT_POSITION_PENALTY=0.000000
1591
PRIMER_RIGHT_POSITION_PENALTY=0.000000
1592
PRIMER_LEFT_END_STABILITY=9.7000
1593
PRIMER_RIGHT_END_STABILITY=8.2000
1594
PRIMER_PAIR_COMPL_ANY=6.00
1595
PRIMER_PAIR_COMPL_END=3.00
1596
PRIMER_PRODUCT_SIZE=4726
1597
PRIMER_PAIR_PENALTY_1=10.1546
1598
PRIMER_LEFT_1_PENALTY=3.573461
1599
PRIMER_RIGHT_1_PENALTY=6.581149
1600
PRIMER_LEFT_1_SEQUENCE=ATGCCACACGAAGAGCTGC
1601
PRIMER_RIGHT_1_SEQUENCE=CACTTTCCATAGAACTTCTTGTTCA
1602
PRIMER_LEFT_1=222,19
1603
PRIMER_RIGHT_1=4948,25
1604
PRIMER_LEFT_1_TM=62.573
1605
PRIMER_RIGHT_1_TM=58.919
1606
PRIMER_LEFT_1_GC_PERCENT=57.895
1607
PRIMER_RIGHT_1_GC_PERCENT=36.000
1608
PRIMER_LEFT_1_SELF_ANY=4.00
1609
PRIMER_RIGHT_1_SELF_ANY=7.00
1610
PRIMER_LEFT_1_SELF_END=3.00
1611
PRIMER_RIGHT_1_SELF_END=3.00
1612
PRIMER_LEFT_1_POSITION_PENALTY=0.000000
1613
PRIMER_RIGHT_1_POSITION_PENALTY=0.500000
1614
PRIMER_LEFT_1_END_STABILITY=9.7000
1615
PRIMER_RIGHT_1_END_STABILITY=6.7000
1616
PRIMER_PAIR_1_COMPL_ANY=6.00
1617
PRIMER_PAIR_1_COMPL_END=3.00
1618
PRIMER_PRODUCT_SIZE_1=4727
1619
PRIMER_PAIR_PENALTY_2=11.1029
1620
PRIMER_LEFT_2_PENALTY=3.573461
1621
PRIMER_RIGHT_2_PENALTY=7.529417
1622
PRIMER_LEFT_2_SEQUENCE=ATGCCACACGAAGAGCTGC
1623
PRIMER_RIGHT_2_SEQUENCE=TCACTTTCCATAGAACTTCTTGTTCA
1624
PRIMER_LEFT_2=222,19
1625
PRIMER_RIGHT_2=4949,26
1626
PRIMER_LEFT_2_TM=62.573
1627
PRIMER_RIGHT_2_TM=60.529
1628
PRIMER_LEFT_2_GC_PERCENT=57.895
1629
PRIMER_RIGHT_2_GC_PERCENT=34.615
1630
PRIMER_LEFT_2_SELF_ANY=4.00
1631
PRIMER_RIGHT_2_SELF_ANY=7.00
1632
PRIMER_LEFT_2_SELF_END=3.00
1633
PRIMER_RIGHT_2_SELF_END=3.00
1634
PRIMER_LEFT_2_POSITION_PENALTY=0.000000
1635
PRIMER_RIGHT_2_POSITION_PENALTY=1.000000
1636
PRIMER_LEFT_2_END_STABILITY=9.7000
1637
PRIMER_RIGHT_2_END_STABILITY=6.7000
1638
PRIMER_PAIR_2_COMPL_ANY=6.00
1639
PRIMER_PAIR_2_COMPL_END=3.00
1640
PRIMER_PRODUCT_SIZE_2=4728
1641
PRIMER_PAIR_PENALTY_3=11.4796
1642
PRIMER_LEFT_3_PENALTY=3.573461
1643
PRIMER_RIGHT_3_PENALTY=7.906112
1644
PRIMER_LEFT_3_SEQUENCE=ATGCCACACGAAGAGCTGC
1645
PRIMER_RIGHT_3_SEQUENCE=TCACTTTCCATAGAACTTCTTGTTC
1646
PRIMER_LEFT_3=222,19
1647
PRIMER_RIGHT_3=4949,25
1648
PRIMER_LEFT_3_TM=62.573
1649
PRIMER_RIGHT_3_TM=58.094
1650
PRIMER_LEFT_3_GC_PERCENT=57.895
1651
PRIMER_RIGHT_3_GC_PERCENT=36.000
1652
PRIMER_LEFT_3_SELF_ANY=4.00
1653
PRIMER_RIGHT_3_SELF_ANY=7.00
1654
PRIMER_LEFT_3_SELF_END=3.00
1655
PRIMER_RIGHT_3_SELF_END=3.00
1656
PRIMER_LEFT_3_POSITION_PENALTY=0.000000
1657
PRIMER_RIGHT_3_POSITION_PENALTY=1.000000
1658
PRIMER_LEFT_3_END_STABILITY=9.7000
1659
PRIMER_RIGHT_3_END_STABILITY=6.7000
1660
PRIMER_PAIR_3_COMPL_ANY=6.00
1661
PRIMER_PAIR_3_COMPL_END=2.00
1662
PRIMER_PRODUCT_SIZE_3=4728
1663
PRIMER_PAIR_PENALTY_4=11.7334
1664
PRIMER_LEFT_4_PENALTY=3.573461
1665
PRIMER_RIGHT_4_PENALTY=8.159919
1666
PRIMER_LEFT_4_SEQUENCE=ATGCCACACGAAGAGCTGC
1667
PRIMER_RIGHT_4_SEQUENCE=GGCACTTCACTTTCCATAGAACT
1668
PRIMER_LEFT_4=222,19
1669
PRIMER_RIGHT_4=4955,23
1670
PRIMER_LEFT_4_TM=62.573
1671
PRIMER_RIGHT_4_TM=58.840
1672
PRIMER_LEFT_4_GC_PERCENT=57.895
1673
PRIMER_RIGHT_4_GC_PERCENT=43.478
1674
PRIMER_LEFT_4_SELF_ANY=4.00
1675
PRIMER_RIGHT_4_SELF_ANY=6.00
1676
PRIMER_LEFT_4_SELF_END=3.00
1677
PRIMER_RIGHT_4_SELF_END=2.00
1678
PRIMER_LEFT_4_POSITION_PENALTY=0.000000
1679
PRIMER_RIGHT_4_POSITION_PENALTY=4.000000
1680
PRIMER_LEFT_4_END_STABILITY=9.7000
1681
PRIMER_RIGHT_4_END_STABILITY=6.4000
1682
PRIMER_PAIR_4_COMPL_ANY=5.00
1683
PRIMER_PAIR_4_COMPL_END=1.00
1684
PRIMER_PRODUCT_SIZE_4=4734
1685
PRIMER_PAIR_PENALTY_5=11.7730
1686
PRIMER_LEFT_5_PENALTY=3.573461
1687
PRIMER_RIGHT_5_PENALTY=8.199569
1688
PRIMER_LEFT_5_SEQUENCE=ATGCCACACGAAGAGCTGC
1689
PRIMER_RIGHT_5_SEQUENCE=TTCACTTTCCATAGAACTTCTTGTTC
1690
PRIMER_LEFT_5=222,19
1691
PRIMER_RIGHT_5=4950,26
1692
PRIMER_LEFT_5_TM=62.573
1693
PRIMER_RIGHT_5_TM=59.300
1694
PRIMER_LEFT_5_GC_PERCENT=57.895
1695
PRIMER_RIGHT_5_GC_PERCENT=34.615
1696
PRIMER_LEFT_5_SELF_ANY=4.00
1697
PRIMER_RIGHT_5_SELF_ANY=7.00
1698
PRIMER_LEFT_5_SELF_END=3.00
1699
PRIMER_RIGHT_5_SELF_END=3.00
1700
PRIMER_LEFT_5_POSITION_PENALTY=0.000000
1701
PRIMER_RIGHT_5_POSITION_PENALTY=1.500000
1702
PRIMER_LEFT_5_END_STABILITY=9.7000
1703
PRIMER_RIGHT_5_END_STABILITY=6.7000
1704
PRIMER_PAIR_5_COMPL_ANY=6.00
1705
PRIMER_PAIR_5_COMPL_END=2.00
1706
PRIMER_PRODUCT_SIZE_5=4729
1707
PRIMER_PAIR_PENALTY_6=12.3404
1708
PRIMER_LEFT_6_PENALTY=3.573461
1709
PRIMER_RIGHT_6_PENALTY=8.766928
1710
PRIMER_LEFT_6_SEQUENCE=ATGCCACACGAAGAGCTGC
1711
PRIMER_RIGHT_6_SEQUENCE=AGGCACTTCACTTTCCATAGAACT
1712
PRIMER_LEFT_6=222,19
1713
PRIMER_RIGHT_6=4956,24
1714
PRIMER_LEFT_6_TM=62.573
1715
PRIMER_RIGHT_6_TM=59.733
1716
PRIMER_LEFT_6_GC_PERCENT=57.895
1717
PRIMER_RIGHT_6_GC_PERCENT=41.667
1718
PRIMER_LEFT_6_SELF_ANY=4.00
1719
PRIMER_RIGHT_6_SELF_ANY=6.00
1720
PRIMER_LEFT_6_SELF_END=3.00
1721
PRIMER_RIGHT_6_SELF_END=2.00
1722
PRIMER_LEFT_6_POSITION_PENALTY=0.000000
1723
PRIMER_RIGHT_6_POSITION_PENALTY=4.500000
1724
PRIMER_LEFT_6_END_STABILITY=9.7000
1725
PRIMER_RIGHT_6_END_STABILITY=6.4000
1726
PRIMER_PAIR_6_COMPL_ANY=5.00
1727
PRIMER_PAIR_6_COMPL_END=1.00
1728
PRIMER_PRODUCT_SIZE_6=4735
1729
PRIMER_PAIR_PENALTY_7=12.3811
1730
PRIMER_LEFT_7_PENALTY=3.573461
1731
PRIMER_RIGHT_7_PENALTY=8.807615
1732
PRIMER_LEFT_7_SEQUENCE=ATGCCACACGAAGAGCTGC
1733
PRIMER_RIGHT_7_SEQUENCE=TTCACTTTCCATAGAACTTCTTGTT
1734
PRIMER_LEFT_7=222,19
1735
PRIMER_RIGHT_7=4950,25
1736
PRIMER_LEFT_7_TM=62.573
1737
PRIMER_RIGHT_7_TM=57.692
1738
PRIMER_LEFT_7_GC_PERCENT=57.895
1739
PRIMER_RIGHT_7_GC_PERCENT=32.000
1740
PRIMER_LEFT_7_SELF_ANY=4.00
1741
PRIMER_RIGHT_7_SELF_ANY=7.00
1742
PRIMER_LEFT_7_SELF_END=3.00
1743
PRIMER_RIGHT_7_SELF_END=3.00
1744
PRIMER_LEFT_7_POSITION_PENALTY=0.000000
1745
PRIMER_RIGHT_7_POSITION_PENALTY=1.500000
1746
PRIMER_LEFT_7_END_STABILITY=9.7000
1747
PRIMER_RIGHT_7_END_STABILITY=7.0000
1748
PRIMER_PAIR_7_COMPL_ANY=6.00
1749
PRIMER_PAIR_7_COMPL_END=3.00
1750
PRIMER_PRODUCT_SIZE_7=4729
1751
PRIMER_PAIR_PENALTY_8=12.6598
1752
PRIMER_LEFT_8_PENALTY=3.573461
1753
PRIMER_RIGHT_8_PENALTY=9.086339
1754
PRIMER_LEFT_8_SEQUENCE=ATGCCACACGAAGAGCTGC
1755
PRIMER_RIGHT_8_SEQUENCE=TCTGTAGGCACTTCACTTTCCA
1756
PRIMER_LEFT_8=222,19
1757
PRIMER_RIGHT_8=4961,22
1758
PRIMER_LEFT_8_TM=62.573
1759
PRIMER_RIGHT_8_TM=59.914
1760
PRIMER_LEFT_8_GC_PERCENT=57.895
1761
PRIMER_RIGHT_8_GC_PERCENT=45.455
1762
PRIMER_LEFT_8_SELF_ANY=4.00
1763
PRIMER_RIGHT_8_SELF_ANY=4.00
1764
PRIMER_LEFT_8_SELF_END=3.00
1765
PRIMER_RIGHT_8_SELF_END=0.00
1766
PRIMER_LEFT_8_POSITION_PENALTY=0.000000
1767
PRIMER_RIGHT_8_POSITION_PENALTY=7.000000
1768
PRIMER_LEFT_8_END_STABILITY=9.7000
1769
PRIMER_RIGHT_8_END_STABILITY=8.5000
1770
PRIMER_PAIR_8_COMPL_ANY=5.00
1771
PRIMER_PAIR_8_COMPL_END=2.00
1772
PRIMER_PRODUCT_SIZE_8=4740
1773
PRIMER_PAIR_PENALTY_9=12.6743
1774
PRIMER_LEFT_9_PENALTY=3.573461
1775
PRIMER_RIGHT_9_PENALTY=9.100880
1776
PRIMER_LEFT_9_SEQUENCE=ATGCCACACGAAGAGCTGC
1777
PRIMER_RIGHT_9_SEQUENCE=CTTCACTTTCCATAGAACTTCTTGTTC
1778
PRIMER_LEFT_9=222,19
1779
PRIMER_RIGHT_9=4951,27
1780
PRIMER_LEFT_9_TM=62.573
1781
PRIMER_RIGHT_9_TM=60.101
1782
PRIMER_LEFT_9_GC_PERCENT=57.895
1783
PRIMER_RIGHT_9_GC_PERCENT=37.037
1784
PRIMER_LEFT_9_SELF_ANY=4.00
1785
PRIMER_RIGHT_9_SELF_ANY=7.00
1786
PRIMER_LEFT_9_SELF_END=3.00
1787
PRIMER_RIGHT_9_SELF_END=3.00
1788
PRIMER_LEFT_9_POSITION_PENALTY=0.000000
1789
PRIMER_RIGHT_9_POSITION_PENALTY=2.000000
1790
PRIMER_LEFT_9_END_STABILITY=9.7000
1791
PRIMER_RIGHT_9_END_STABILITY=6.7000
1792
PRIMER_PAIR_9_COMPL_ANY=6.00
1793
PRIMER_PAIR_9_COMPL_END=2.00
1794
PRIMER_PRODUCT_SIZE_9=4730
1795
PRIMER_PAIR_PENALTY_10=12.9973
1796
PRIMER_LEFT_10_PENALTY=3.573461
1797
PRIMER_RIGHT_10_PENALTY=9.423820
1798
PRIMER_LEFT_10_SEQUENCE=ATGCCACACGAAGAGCTGC
1799
PRIMER_RIGHT_10_SEQUENCE=CTTCACTTTCCATAGAACTTCTTGTT
1800
PRIMER_LEFT_10=222,19
1801
PRIMER_RIGHT_10=4951,26
1802
PRIMER_LEFT_10_TM=62.573
1803
PRIMER_RIGHT_10_TM=58.576
1804
PRIMER_LEFT_10_GC_PERCENT=57.895
1805
PRIMER_RIGHT_10_GC_PERCENT=34.615
1806
PRIMER_LEFT_10_SELF_ANY=4.00
1807
PRIMER_RIGHT_10_SELF_ANY=7.00
1808
PRIMER_LEFT_10_SELF_END=3.00
1809
PRIMER_RIGHT_10_SELF_END=3.00
1810
PRIMER_LEFT_10_POSITION_PENALTY=0.000000
1811
PRIMER_RIGHT_10_POSITION_PENALTY=2.000000
1812
PRIMER_LEFT_10_END_STABILITY=9.7000
1813
PRIMER_RIGHT_10_END_STABILITY=7.0000
1814
PRIMER_PAIR_10_COMPL_ANY=6.00
1815
PRIMER_PAIR_10_COMPL_END=3.00
1816
PRIMER_PRODUCT_SIZE_10=4730
1817
PRIMER_PAIR_PENALTY_11=13.0507
1818
PRIMER_LEFT_11_PENALTY=3.573461
1819
PRIMER_RIGHT_11_PENALTY=9.477278
1820
PRIMER_LEFT_11_SEQUENCE=ATGCCACACGAAGAGCTGC
1821
PRIMER_RIGHT_11_SEQUENCE=CTGTAGGCACTTCACTTTCCA
1822
PRIMER_LEFT_11=222,19
1823
PRIMER_RIGHT_11=4960,21
1824
PRIMER_LEFT_11_TM=62.573
1825
PRIMER_RIGHT_11_TM=58.023
1826
PRIMER_LEFT_11_GC_PERCENT=57.895
1827
PRIMER_RIGHT_11_GC_PERCENT=47.619
1828
PRIMER_LEFT_11_SELF_ANY=4.00
1829
PRIMER_RIGHT_11_SELF_ANY=4.00
1830
PRIMER_LEFT_11_SELF_END=3.00
1831
PRIMER_RIGHT_11_SELF_END=0.00
1832
PRIMER_LEFT_11_POSITION_PENALTY=0.000000
1833
PRIMER_RIGHT_11_POSITION_PENALTY=6.500000
1834
PRIMER_LEFT_11_END_STABILITY=9.7000
1835
PRIMER_RIGHT_11_END_STABILITY=8.5000
1836
PRIMER_PAIR_11_COMPL_ANY=5.00
1837
PRIMER_PAIR_11_COMPL_END=2.00
1838
PRIMER_PRODUCT_SIZE_11=4739
1839
PRIMER_PAIR_PENALTY_12=13.2295
1840
PRIMER_LEFT_12_PENALTY=3.573461
1841
PRIMER_RIGHT_12_PENALTY=9.655999
1842
PRIMER_LEFT_12_SEQUENCE=ATGCCACACGAAGAGCTGC
1843
PRIMER_RIGHT_12_SEQUENCE=TGTAGGCACTTCACTTTCCAT
1844
PRIMER_LEFT_12=222,19
1845
PRIMER_RIGHT_12=4959,21
1846
PRIMER_LEFT_12_TM=62.573
1847
PRIMER_RIGHT_12_TM=57.344
1848
PRIMER_LEFT_12_GC_PERCENT=57.895
1849
PRIMER_RIGHT_12_GC_PERCENT=42.857
1850
PRIMER_LEFT_12_SELF_ANY=4.00
1851
PRIMER_RIGHT_12_SELF_ANY=4.00
1852
PRIMER_LEFT_12_SELF_END=3.00
1853
PRIMER_RIGHT_12_SELF_END=2.00
1854
PRIMER_LEFT_12_POSITION_PENALTY=0.000000
1855
PRIMER_RIGHT_12_POSITION_PENALTY=6.000000
1856
PRIMER_LEFT_12_END_STABILITY=9.7000
1857
PRIMER_RIGHT_12_END_STABILITY=8.1000
1858
PRIMER_PAIR_12_COMPL_ANY=5.00
1859
PRIMER_PAIR_12_COMPL_END=3.00
1860
PRIMER_PRODUCT_SIZE_12=4738
1861
PRIMER_PAIR_PENALTY_13=13.3361
1862
PRIMER_LEFT_13_PENALTY=3.573461
1863
PRIMER_RIGHT_13_PENALTY=9.762661
1864
PRIMER_LEFT_13_SEQUENCE=ATGCCACACGAAGAGCTGC
1865
PRIMER_RIGHT_13_SEQUENCE=ACTTTCCATAGAACTTCTTGTTCAGCA
1866
PRIMER_LEFT_13=222,19
1867
PRIMER_RIGHT_13=4947,27
1868
PRIMER_LEFT_13_TM=62.573
1869
PRIMER_RIGHT_13_TM=62.763
1870
PRIMER_LEFT_13_GC_PERCENT=57.895
1871
PRIMER_RIGHT_13_GC_PERCENT=37.037
1872
PRIMER_LEFT_13_SELF_ANY=4.00
1873
PRIMER_RIGHT_13_SELF_ANY=7.00
1874
PRIMER_LEFT_13_SELF_END=3.00
1875
PRIMER_RIGHT_13_SELF_END=3.00
1876
PRIMER_LEFT_13_POSITION_PENALTY=0.000000
1877
PRIMER_RIGHT_13_POSITION_PENALTY=0.000000
1878
PRIMER_LEFT_13_END_STABILITY=9.7000
1879
PRIMER_RIGHT_13_END_STABILITY=8.5000
1880
PRIMER_PAIR_13_COMPL_ANY=6.00
1881
PRIMER_PAIR_13_COMPL_END=3.00
1882
PRIMER_PRODUCT_SIZE_13=4726
1883
PRIMER_PAIR_PENALTY_14=13.6715
1884
PRIMER_LEFT_14_PENALTY=3.573461
1885
PRIMER_RIGHT_14_PENALTY=10.098012
1886
PRIMER_LEFT_14_SEQUENCE=ATGCCACACGAAGAGCTGC
1887
PRIMER_RIGHT_14_SEQUENCE=TTCACTTTCCATAGAACTTCTTGTTCA
1888
PRIMER_LEFT_14=222,19
1889
PRIMER_RIGHT_14=4950,27
1890
PRIMER_LEFT_14_TM=62.573
1891
PRIMER_RIGHT_14_TM=61.598
1892
PRIMER_LEFT_14_GC_PERCENT=57.895
1893
PRIMER_RIGHT_14_GC_PERCENT=33.333
1894
PRIMER_LEFT_14_SELF_ANY=4.00
1895
PRIMER_RIGHT_14_SELF_ANY=7.00
1896
PRIMER_LEFT_14_SELF_END=3.00
1897
PRIMER_RIGHT_14_SELF_END=3.00
1898
PRIMER_LEFT_14_POSITION_PENALTY=0.000000
1899
PRIMER_RIGHT_14_POSITION_PENALTY=1.500000
1900
PRIMER_LEFT_14_END_STABILITY=9.7000
1901
PRIMER_RIGHT_14_END_STABILITY=6.7000
1902
PRIMER_PAIR_14_COMPL_ANY=6.00
1903
PRIMER_PAIR_14_COMPL_END=3.00
1904
PRIMER_PRODUCT_SIZE_14=4729
1905
PRIMER_PAIR_PENALTY_15=13.6784
1906
PRIMER_LEFT_15_PENALTY=3.573461
1907
PRIMER_RIGHT_15_PENALTY=10.104943
1908
PRIMER_LEFT_15_SEQUENCE=ATGCCACACGAAGAGCTGC
1909
PRIMER_RIGHT_15_SEQUENCE=CTGTAGGCACTTCACTTTCCAT
1910
PRIMER_LEFT_15=222,19
1911
PRIMER_RIGHT_15=4960,22
1912
PRIMER_LEFT_15_TM=62.573
1913
PRIMER_RIGHT_15_TM=58.395
1914
PRIMER_LEFT_15_GC_PERCENT=57.895
1915
PRIMER_RIGHT_15_GC_PERCENT=45.455
1916
PRIMER_LEFT_15_SELF_ANY=4.00
1917
PRIMER_RIGHT_15_SELF_ANY=4.00
1918
PRIMER_LEFT_15_SELF_END=3.00
1919
PRIMER_RIGHT_15_SELF_END=2.00
1920
PRIMER_LEFT_15_POSITION_PENALTY=0.000000
1921
PRIMER_RIGHT_15_POSITION_PENALTY=6.500000
1922
PRIMER_LEFT_15_END_STABILITY=9.7000
1923
PRIMER_RIGHT_15_END_STABILITY=8.1000
1924
PRIMER_PAIR_15_COMPL_ANY=5.00
1925
PRIMER_PAIR_15_COMPL_END=3.00
1926
PRIMER_PRODUCT_SIZE_15=4739
1927
PRIMER_PAIR_PENALTY_16=13.7519
1928
PRIMER_LEFT_16_PENALTY=3.573461
1929
PRIMER_RIGHT_16_PENALTY=10.178426
1930
PRIMER_LEFT_16_SEQUENCE=ATGCCACACGAAGAGCTGC
1931
PRIMER_RIGHT_16_SEQUENCE=TCTGTAGGCACTTCACTTTCCAT
1932
PRIMER_LEFT_16=222,19
1933
PRIMER_RIGHT_16=4961,23
1934
PRIMER_LEFT_16_TM=62.573
1935
PRIMER_RIGHT_16_TM=60.178
1936
PRIMER_LEFT_16_GC_PERCENT=57.895
1937
PRIMER_RIGHT_16_GC_PERCENT=43.478
1938
PRIMER_LEFT_16_SELF_ANY=4.00
1939
PRIMER_RIGHT_16_SELF_ANY=4.00
1940
PRIMER_LEFT_16_SELF_END=3.00
1941
PRIMER_RIGHT_16_SELF_END=2.00
1942
PRIMER_LEFT_16_POSITION_PENALTY=0.000000
1943
PRIMER_RIGHT_16_POSITION_PENALTY=7.000000
1944
PRIMER_LEFT_16_END_STABILITY=9.7000
1945
PRIMER_RIGHT_16_END_STABILITY=8.1000
1946
PRIMER_PAIR_16_COMPL_ANY=5.00
1947
PRIMER_PAIR_16_COMPL_END=3.00
1948
PRIMER_PRODUCT_SIZE_16=4740
1949
PRIMER_PAIR_PENALTY_17=13.7905
1950
PRIMER_LEFT_17_PENALTY=3.573461
1951
PRIMER_RIGHT_17_PENALTY=10.217023
1952
PRIMER_LEFT_17_SEQUENCE=ATGCCACACGAAGAGCTGC
1953
PRIMER_RIGHT_17_SEQUENCE=ACTTCACTTTCCATAGAACTTCTTGTT
1954
PRIMER_LEFT_17=222,19
1955
PRIMER_RIGHT_17=4952,27
1956
PRIMER_LEFT_17_TM=62.573
1957
PRIMER_RIGHT_17_TM=59.283
1958
PRIMER_LEFT_17_GC_PERCENT=57.895
1959
PRIMER_RIGHT_17_GC_PERCENT=33.333
1960
PRIMER_LEFT_17_SELF_ANY=4.00
1961
PRIMER_RIGHT_17_SELF_ANY=7.00
1962
PRIMER_LEFT_17_SELF_END=3.00
1963
PRIMER_RIGHT_17_SELF_END=3.00
1964
PRIMER_LEFT_17_POSITION_PENALTY=0.000000
1965
PRIMER_RIGHT_17_POSITION_PENALTY=2.500000
1966
PRIMER_LEFT_17_END_STABILITY=9.7000
1967
PRIMER_RIGHT_17_END_STABILITY=7.0000
1968
PRIMER_PAIR_17_COMPL_ANY=6.00
1969
PRIMER_PAIR_17_COMPL_END=3.00
1970
PRIMER_PRODUCT_SIZE_17=4731
1971
PRIMER_PAIR_PENALTY_18=13.8224
1972
PRIMER_LEFT_18_PENALTY=3.573461
1973
PRIMER_RIGHT_18_PENALTY=10.248922
1974
PRIMER_LEFT_18_SEQUENCE=ATGCCACACGAAGAGCTGC
1975
PRIMER_RIGHT_18_SEQUENCE=TAGGCACTTCACTTTCCATAGAACT
1976
PRIMER_LEFT_18=222,19
1977
PRIMER_RIGHT_18=4957,25
1978
PRIMER_LEFT_18_TM=62.573
1979
PRIMER_RIGHT_18_TM=59.751
1980
PRIMER_LEFT_18_GC_PERCENT=57.895
1981
PRIMER_RIGHT_18_GC_PERCENT=40.000
1982
PRIMER_LEFT_18_SELF_ANY=4.00
1983
PRIMER_RIGHT_18_SELF_ANY=6.00
1984
PRIMER_LEFT_18_SELF_END=3.00
1985
PRIMER_RIGHT_18_SELF_END=2.00
1986
PRIMER_LEFT_18_POSITION_PENALTY=0.000000
1987
PRIMER_RIGHT_18_POSITION_PENALTY=5.000000
1988
PRIMER_LEFT_18_END_STABILITY=9.7000
1989
PRIMER_RIGHT_18_END_STABILITY=6.4000
1990
PRIMER_PAIR_18_COMPL_ANY=5.00
1991
PRIMER_PAIR_18_COMPL_END=1.00
1992
PRIMER_PRODUCT_SIZE_18=4736
1993
PRIMER_PAIR_PENALTY_19=13.8361
1994
PRIMER_LEFT_19_PENALTY=3.573461
1995
PRIMER_RIGHT_19_PENALTY=10.262661
1996
PRIMER_LEFT_19_SEQUENCE=ATGCCACACGAAGAGCTGC
1997
PRIMER_RIGHT_19_SEQUENCE=CACTTTCCATAGAACTTCTTGTTCAGC
1998
PRIMER_LEFT_19=222,19
1999
PRIMER_RIGHT_19=4948,27
2000
PRIMER_LEFT_19_TM=62.573
2001
PRIMER_RIGHT_19_TM=62.763
2002
PRIMER_LEFT_19_GC_PERCENT=57.895
2003
PRIMER_RIGHT_19_GC_PERCENT=40.741
2004
PRIMER_LEFT_19_SELF_ANY=4.00
2005
PRIMER_RIGHT_19_SELF_ANY=7.00
2006
PRIMER_LEFT_19_SELF_END=3.00
2007
PRIMER_RIGHT_19_SELF_END=3.00
2008
PRIMER_LEFT_19_POSITION_PENALTY=0.000000
2009
PRIMER_RIGHT_19_POSITION_PENALTY=0.500000
2010
PRIMER_LEFT_19_END_STABILITY=9.7000
2011
PRIMER_RIGHT_19_END_STABILITY=8.2000
2012
PRIMER_PAIR_19_COMPL_ANY=6.00
2013
PRIMER_PAIR_19_COMPL_END=3.00
2014
PRIMER_PRODUCT_SIZE_19=4727
2015
=
2016
PRIMER_SEQUENCE_ID=M59040/CD44
2017
PRIMER_COMMENT=Human cell adhesion molecule (CD44) mRNA, complete cds. CDS=29..1114
2018
PRIMER_START_CODON_POSITION=28
2019
SEQUENCE=CCCGCGCCCTCCGTTCGCTCCGGACACCATGGACAAGTTTTGGTGGCACGCAGCCTGGGGACTCTGCCTCGTGCCGCTGAGCCTGGCGCAGATCGATTTGAATATAACCTGCCGCTTTGCAGGTGTATTCCACGTGGAGAAAAATGGTCGCTACAGCATCTCTCGGACGGAGGCCGCTGACCTCTGCAAGGCTTTCAATAGCACCTTGCCCACAATGGCCCAGATGGAGAAAGCTCTGAGCATCGGATTTGAGACCTGCAGGTATGGGTTCATAGAAGGGCATGTGGTGATTCCCCGGATCCACCCCAACTCCATCTGTGCAGCAAACAACACAGGGGTGTACATCCTCACATACAACACCTCCCAGTATGACACATATTGCTTCAATGCTTCAGCTCCACCTGAAGAAGATTGTACATCAGTCACAGACCTGCCCAATGCCTTTGATGGACCAATTACCATAACTATTGTTAACCGTGATGGCACCCGCTATGTCCAGAAAGGAGAATACAGAACGAATCCTGAAGACATCTACCCCAGCAACCCTACTGATGATGACGTGAGCAGCGGCTCCTCCAGTGAAAGGAGCAGCACTTCAGGAGGTTACATCTTTTACACCTTTTCTACTGTACACCCCATCCCAGACGAAGACAGTCCCTGGATCACCGACAGCACAGACAGAATCCCTGCTACCAGAGACCAAGACACATTCCACCCCAGTGGGGGGTCCCATACCACTCATGGATCTGAATCAGATGGACACTCACATGGGAGTCAAGAAGGTGGAGCAAACACAACCTCTGGTCCTATAAGGACACCCCAAATTCCAGAATGGCTGATCATCTTGGCATCCCTCTTGGCCTTGGCTTTGATTCTTGCAGTTTGCATTGCAGTCAACAGTCGAAGAAGGTGTGGGCAGAAGAAAAAGCTAGTGATCAACAGTGGCAATGGAGCTGTGGAGGACAGAAAGCCAAGTGGACTCAACGGAGAGGCCAGCAAGTCTCAGGAAATGGTGCATTTGGTGAACAAGGAGTCGTCAGAAACTCCAGACCAGTTTATGACAGCTGATGAGACAAGGAACCTGCAGAATGTGGACATGAAGATTGGGGTGTAACACCTACACCATTATCTTGGAAAGAAACAACCGTTGGAAACATAACCATTACAGGGAGCTGGGACACTTAACAGATGCAATGTGCTACTGATTGTTTCATTGCGAATCTTTTTTAGCATAAAATTTTCTACTCTTTTTGTTTTTTGTGTTTTGTTCTTTAAAGTCAGGTCCAATTTGTAAAAACAGCATTGCTTTGTAAATTAGGGCCCAATTAATAATCAGCAAGAATTTGATCGTTCAGTTCCACTTGGAGGCCTTCATCCTCGGGTGTGCTATGGATGGCTTCTAACAAAAACTACACATATGTATTCCTGATCGCCAACCTTTCCCCCACCAGCTAAGGACATTTCCCAGGGTTAATAGGGCCTGGTCCCTGGGAGGAAATTTGAATGGGTCCATTTTGCCCTTCCATAGCCTAATCCCTGGGCATTGCTTTCCACTGAGGTTGGGGTGTACTAGTTACACATCTTCAACAGACCCCCTCTAGAAATTTTTCAGATGCTTCTGGGAGACACCAAAGGGTGAAGCTATTTATCTGTAGTAAACTATTTATCTGTGTTTTTGAAATATTAAACCCTGGATCAGTCCTTTGATCAGTATAATTTTTTAAAGTTACTTTGTCAGAGGCACAAAAGGGTTTAAACTGATTCATAATAAATATCTGTACTTCTTCGATCTTC
2020
PRIMER_LEFT_EXPLAIN=considered 15178, would not amplify any of the ORF 11930, GC content failed 2, low tm 495, high tm 1516, high end compl 9, long poly-x seq 22,high 3' stability 27, ok 1177
2021
PRIMER_RIGHT_EXPLAIN=considered 14630, GC content failed 362, low tm 3262, high tm 5355, high any compl 3, high end compl 2, long poly-x seq 73,high 3' stability 60, ok 5513
2022
PRIMER_PAIR_EXPLAIN=considered 60, high end compl 6, ok 54
2023
PRIMER_STOP_CODON_POSITION=1111
2024
PRIMER_PAIR_PENALTY=1.6996
2025
PRIMER_LEFT_PENALTY=1.335843
2026
PRIMER_RIGHT_PENALTY=0.363753
2027
PRIMER_LEFT_SEQUENCE=ATGGACAAGTTTTGGTGGCA
2028
PRIMER_RIGHT_SEQUENCE=ACACCCCAATCTTCATGTCC
2029
PRIMER_LEFT=28,20
2030
PRIMER_RIGHT=1111,20
2031
PRIMER_LEFT_TM=61.336
2032
PRIMER_RIGHT_TM=59.636
2033
PRIMER_LEFT_GC_PERCENT=45.000
2034
PRIMER_RIGHT_GC_PERCENT=50.000
2035
PRIMER_LEFT_SELF_ANY=3.00
2036
PRIMER_RIGHT_SELF_ANY=4.00
2037
PRIMER_LEFT_SELF_END=3.00
2038
PRIMER_RIGHT_SELF_END=0.00
2039
PRIMER_LEFT_POSITION_PENALTY=0.000000
2040
PRIMER_RIGHT_POSITION_PENALTY=0.000000
2041
PRIMER_LEFT_END_STABILITY=10.0000
2042
PRIMER_RIGHT_END_STABILITY=7.9000
2043
PRIMER_PAIR_COMPL_ANY=5.00
2044
PRIMER_PAIR_COMPL_END=2.00
2045
PRIMER_PRODUCT_SIZE=1084
2046
PRIMER_PAIR_PENALTY_1=2.9624
2047
PRIMER_LEFT_1_PENALTY=2.598622
2048
PRIMER_RIGHT_1_PENALTY=0.363753
2049
PRIMER_LEFT_1_SEQUENCE=ATGGACAAGTTTTGGTGGC
2050
PRIMER_RIGHT_1_SEQUENCE=ACACCCCAATCTTCATGTCC
2051
PRIMER_LEFT_1=28,19
2052
PRIMER_RIGHT_1=1111,20
2053
PRIMER_LEFT_1_TM=58.401
2054
PRIMER_RIGHT_1_TM=59.636
2055
PRIMER_LEFT_1_GC_PERCENT=47.368
2056
PRIMER_RIGHT_1_GC_PERCENT=50.000
2057
PRIMER_LEFT_1_SELF_ANY=3.00
2058
PRIMER_RIGHT_1_SELF_ANY=4.00
2059
PRIMER_LEFT_1_SELF_END=2.00
2060
PRIMER_RIGHT_1_SELF_END=0.00
2061
PRIMER_LEFT_1_POSITION_PENALTY=0.000000
2062
PRIMER_RIGHT_1_POSITION_PENALTY=0.000000
2063
PRIMER_LEFT_1_END_STABILITY=9.4000
2064
PRIMER_RIGHT_1_END_STABILITY=7.9000
2065
PRIMER_PAIR_1_COMPL_ANY=5.00
2066
PRIMER_PAIR_1_COMPL_END=2.00
2067
PRIMER_PRODUCT_SIZE_1=1084
2068
PRIMER_PAIR_PENALTY_2=3.1742
2069
PRIMER_LEFT_2_PENALTY=1.335843
2070
PRIMER_RIGHT_2_PENALTY=1.838405
2071
PRIMER_LEFT_2_SEQUENCE=ATGGACAAGTTTTGGTGGCA
2072
PRIMER_RIGHT_2_SEQUENCE=TACACCCCAATCTTCATGTCC
2073
PRIMER_LEFT_2=28,20
2074
PRIMER_RIGHT_2=1112,21
2075
PRIMER_LEFT_2_TM=61.336
2076
PRIMER_RIGHT_2_TM=59.662
2077
PRIMER_LEFT_2_GC_PERCENT=45.000
2078
PRIMER_RIGHT_2_GC_PERCENT=47.619
2079
PRIMER_LEFT_2_SELF_ANY=3.00
2080
PRIMER_RIGHT_2_SELF_ANY=4.00
2081
PRIMER_LEFT_2_SELF_END=3.00
2082
PRIMER_RIGHT_2_SELF_END=0.00
2083
PRIMER_LEFT_2_POSITION_PENALTY=0.000000
2084
PRIMER_RIGHT_2_POSITION_PENALTY=0.500000
2085
PRIMER_LEFT_2_END_STABILITY=10.0000
2086
PRIMER_RIGHT_2_END_STABILITY=7.9000
2087
PRIMER_PAIR_2_COMPL_ANY=5.00
2088
PRIMER_PAIR_2_COMPL_END=2.00
2089
PRIMER_PRODUCT_SIZE_2=1085
2090
PRIMER_PAIR_PENALTY_3=4.4370
2091
PRIMER_LEFT_3_PENALTY=2.598622
2092
PRIMER_RIGHT_3_PENALTY=1.838405
2093
PRIMER_LEFT_3_SEQUENCE=ATGGACAAGTTTTGGTGGC
2094
PRIMER_RIGHT_3_SEQUENCE=TACACCCCAATCTTCATGTCC
2095
PRIMER_LEFT_3=28,19
2096
PRIMER_RIGHT_3=1112,21
2097
PRIMER_LEFT_3_TM=58.401
2098
PRIMER_RIGHT_3_TM=59.662
2099
PRIMER_LEFT_3_GC_PERCENT=47.368
2100
PRIMER_RIGHT_3_GC_PERCENT=47.619
2101
PRIMER_LEFT_3_SELF_ANY=3.00
2102
PRIMER_RIGHT_3_SELF_ANY=4.00
2103
PRIMER_LEFT_3_SELF_END=2.00
2104
PRIMER_RIGHT_3_SELF_END=0.00
2105
PRIMER_LEFT_3_POSITION_PENALTY=0.000000
2106
PRIMER_RIGHT_3_POSITION_PENALTY=0.500000
2107
PRIMER_LEFT_3_END_STABILITY=9.4000
2108
PRIMER_RIGHT_3_END_STABILITY=7.9000
2109
PRIMER_PAIR_3_COMPL_ANY=5.00
2110
PRIMER_PAIR_3_COMPL_END=2.00
2111
PRIMER_PRODUCT_SIZE_3=1085
2112
PRIMER_PAIR_PENALTY_4=4.8500
2113
PRIMER_LEFT_4_PENALTY=1.335843
2114
PRIMER_RIGHT_4_PENALTY=3.514144
2115
PRIMER_LEFT_4_SEQUENCE=ATGGACAAGTTTTGGTGGCA
2116
PRIMER_RIGHT_4_SEQUENCE=ACACCCCAATCTTCATGTCCA
2117
PRIMER_LEFT_4=28,20
2118
PRIMER_RIGHT_4=1111,21
2119
PRIMER_LEFT_4_TM=61.336
2120
PRIMER_RIGHT_4_TM=62.514
2121
PRIMER_LEFT_4_GC_PERCENT=45.000
2122
PRIMER_RIGHT_4_GC_PERCENT=47.619
2123
PRIMER_LEFT_4_SELF_ANY=3.00
2124
PRIMER_RIGHT_4_SELF_ANY=4.00
2125
PRIMER_LEFT_4_SELF_END=3.00
2126
PRIMER_RIGHT_4_SELF_END=2.00
2127
PRIMER_LEFT_4_POSITION_PENALTY=0.000000
2128
PRIMER_RIGHT_4_POSITION_PENALTY=0.000000
2129
PRIMER_LEFT_4_END_STABILITY=10.0000
2130
PRIMER_RIGHT_4_END_STABILITY=7.9000
2131
PRIMER_PAIR_4_COMPL_ANY=6.00
2132
PRIMER_PAIR_4_COMPL_END=3.00
2133
PRIMER_PRODUCT_SIZE_4=1084
2134
PRIMER_PAIR_PENALTY_5=5.3038
2135
PRIMER_LEFT_5_PENALTY=1.335843
2136
PRIMER_RIGHT_5_PENALTY=3.967916
2137
PRIMER_LEFT_5_SEQUENCE=ATGGACAAGTTTTGGTGGCA
2138
PRIMER_RIGHT_5_SEQUENCE=TTACACCCCAATCTTCATGTCC
2139
PRIMER_LEFT_5=28,20
2140
PRIMER_RIGHT_5=1113,22
2141
PRIMER_LEFT_5_TM=61.336
2142
PRIMER_RIGHT_5_TM=60.968
2143
PRIMER_LEFT_5_GC_PERCENT=45.000
2144
PRIMER_RIGHT_5_GC_PERCENT=45.455
2145
PRIMER_LEFT_5_SELF_ANY=3.00
2146
PRIMER_RIGHT_5_SELF_ANY=4.00
2147
PRIMER_LEFT_5_SELF_END=3.00
2148
PRIMER_RIGHT_5_SELF_END=0.00
2149
PRIMER_LEFT_5_POSITION_PENALTY=0.000000
2150
PRIMER_RIGHT_5_POSITION_PENALTY=1.000000
2151
PRIMER_LEFT_5_END_STABILITY=10.0000
2152
PRIMER_RIGHT_5_END_STABILITY=7.9000
2153
PRIMER_PAIR_5_COMPL_ANY=5.00
2154
PRIMER_PAIR_5_COMPL_END=2.00
2155
PRIMER_PRODUCT_SIZE_5=1086
2156
PRIMER_PAIR_PENALTY_6=5.9592
2157
PRIMER_LEFT_6_PENALTY=1.335843
2158
PRIMER_RIGHT_6_PENALTY=4.623327
2159
PRIMER_LEFT_6_SEQUENCE=ATGGACAAGTTTTGGTGGCA
2160
PRIMER_RIGHT_6_SEQUENCE=TTACACCCCAATCTTCATGTC
2161
PRIMER_LEFT_6=28,20
2162
PRIMER_RIGHT_6=1113,21
2163
PRIMER_LEFT_6_TM=61.336
2164
PRIMER_RIGHT_6_TM=57.377
2165
PRIMER_LEFT_6_GC_PERCENT=45.000
2166
PRIMER_RIGHT_6_GC_PERCENT=42.857
2167
PRIMER_LEFT_6_SELF_ANY=3.00
2168
PRIMER_RIGHT_6_SELF_ANY=4.00
2169
PRIMER_LEFT_6_SELF_END=3.00
2170
PRIMER_RIGHT_6_SELF_END=1.00
2171
PRIMER_LEFT_6_POSITION_PENALTY=0.000000
2172
PRIMER_RIGHT_6_POSITION_PENALTY=1.000000
2173
PRIMER_LEFT_6_END_STABILITY=10.0000
2174
PRIMER_RIGHT_6_END_STABILITY=6.3000
2175
PRIMER_PAIR_6_COMPL_ANY=5.00
2176
PRIMER_PAIR_6_COMPL_END=2.00
2177
PRIMER_PRODUCT_SIZE_6=1086
2178
PRIMER_PAIR_PENALTY_7=6.1128
2179
PRIMER_LEFT_7_PENALTY=2.598622
2180
PRIMER_RIGHT_7_PENALTY=3.514144
2181
PRIMER_LEFT_7_SEQUENCE=ATGGACAAGTTTTGGTGGC
2182
PRIMER_RIGHT_7_SEQUENCE=ACACCCCAATCTTCATGTCCA
2183
PRIMER_LEFT_7=28,19
2184
PRIMER_RIGHT_7=1111,21
2185
PRIMER_LEFT_7_TM=58.401
2186
PRIMER_RIGHT_7_TM=62.514
2187
PRIMER_LEFT_7_GC_PERCENT=47.368
2188
PRIMER_RIGHT_7_GC_PERCENT=47.619
2189
PRIMER_LEFT_7_SELF_ANY=3.00
2190
PRIMER_RIGHT_7_SELF_ANY=4.00
2191
PRIMER_LEFT_7_SELF_END=2.00
2192
PRIMER_RIGHT_7_SELF_END=2.00
2193
PRIMER_LEFT_7_POSITION_PENALTY=0.000000
2194
PRIMER_RIGHT_7_POSITION_PENALTY=0.000000
2195
PRIMER_LEFT_7_END_STABILITY=9.4000
2196
PRIMER_RIGHT_7_END_STABILITY=7.9000
2197
PRIMER_PAIR_7_COMPL_ANY=6.00
2198
PRIMER_PAIR_7_COMPL_END=3.00
2199
PRIMER_PRODUCT_SIZE_7=1084
2200
PRIMER_PAIR_PENALTY_8=6.1174
2201
PRIMER_LEFT_8_PENALTY=1.335843
2202
PRIMER_RIGHT_8_PENALTY=4.781508
2203
PRIMER_LEFT_8_SEQUENCE=ATGGACAAGTTTTGGTGGCA
2204
PRIMER_RIGHT_8_SEQUENCE=TGTTACACCCCAATCTTCATGT
2205
PRIMER_LEFT_8=28,20
2206
PRIMER_RIGHT_8=1115,22
2207
PRIMER_LEFT_8_TM=61.336
2208
PRIMER_RIGHT_8_TM=59.218
2209
PRIMER_LEFT_8_GC_PERCENT=45.000
2210
PRIMER_RIGHT_8_GC_PERCENT=40.909
2211
PRIMER_LEFT_8_SELF_ANY=3.00
2212
PRIMER_RIGHT_8_SELF_ANY=5.00
2213
PRIMER_LEFT_8_SELF_END=3.00
2214
PRIMER_RIGHT_8_SELF_END=2.00
2215
PRIMER_LEFT_8_POSITION_PENALTY=0.000000
2216
PRIMER_RIGHT_8_POSITION_PENALTY=2.000000
2217
PRIMER_LEFT_8_END_STABILITY=10.0000
2218
PRIMER_RIGHT_8_END_STABILITY=6.6000
2219
PRIMER_PAIR_8_COMPL_ANY=5.00
2220
PRIMER_PAIR_8_COMPL_END=2.00
2221
PRIMER_PRODUCT_SIZE_8=1088
2222
PRIMER_PAIR_PENALTY_9=6.2650
2223
PRIMER_LEFT_9_PENALTY=1.335843
2224
PRIMER_RIGHT_9_PENALTY=4.929154
2225
PRIMER_LEFT_9_SEQUENCE=ATGGACAAGTTTTGGTGGCA
2226
PRIMER_RIGHT_9_SEQUENCE=TACACCCCAATCTTCATGTCCA
2227
PRIMER_LEFT_9=28,20
2228
PRIMER_RIGHT_9=1112,22
2229
PRIMER_LEFT_9_TM=61.336
2230
PRIMER_RIGHT_9_TM=62.429
2231
PRIMER_LEFT_9_GC_PERCENT=45.000
2232
PRIMER_RIGHT_9_GC_PERCENT=45.455
2233
PRIMER_LEFT_9_SELF_ANY=3.00
2234
PRIMER_RIGHT_9_SELF_ANY=4.00
2235
PRIMER_LEFT_9_SELF_END=3.00
2236
PRIMER_RIGHT_9_SELF_END=2.00
2237
PRIMER_LEFT_9_POSITION_PENALTY=0.000000
2238
PRIMER_RIGHT_9_POSITION_PENALTY=0.500000
2239
PRIMER_LEFT_9_END_STABILITY=10.0000
2240
PRIMER_RIGHT_9_END_STABILITY=7.9000
2241
PRIMER_PAIR_9_COMPL_ANY=6.00
2242
PRIMER_PAIR_9_COMPL_END=3.00
2243
PRIMER_PRODUCT_SIZE_9=1085
2244
PRIMER_PAIR_PENALTY_10=6.5429
2245
PRIMER_LEFT_10_PENALTY=1.335843
2246
PRIMER_RIGHT_10_PENALTY=5.207042
2247
PRIMER_LEFT_10_SEQUENCE=ATGGACAAGTTTTGGTGGCA
2248
PRIMER_RIGHT_10_SEQUENCE=GTTACACCCCAATCTTCATGTC
2249
PRIMER_LEFT_10=28,20
2250
PRIMER_RIGHT_10=1114,22
2251
PRIMER_LEFT_10_TM=61.336
2252
PRIMER_RIGHT_10_TM=58.293
2253
PRIMER_LEFT_10_GC_PERCENT=45.000
2254
PRIMER_RIGHT_10_GC_PERCENT=45.455
2255
PRIMER_LEFT_10_SELF_ANY=3.00
2256
PRIMER_RIGHT_10_SELF_ANY=4.00
2257
PRIMER_LEFT_10_SELF_END=3.00
2258
PRIMER_RIGHT_10_SELF_END=1.00
2259
PRIMER_LEFT_10_POSITION_PENALTY=0.000000
2260
PRIMER_RIGHT_10_POSITION_PENALTY=1.500000
2261
PRIMER_LEFT_10_END_STABILITY=10.0000
2262
PRIMER_RIGHT_10_END_STABILITY=6.3000
2263
PRIMER_PAIR_10_COMPL_ANY=5.00
2264
PRIMER_PAIR_10_COMPL_END=2.00
2265
PRIMER_PRODUCT_SIZE_10=1087
2266
PRIMER_PAIR_PENALTY_11=6.5665
2267
PRIMER_LEFT_11_PENALTY=2.598622
2268
PRIMER_RIGHT_11_PENALTY=3.967916
2269
PRIMER_LEFT_11_SEQUENCE=ATGGACAAGTTTTGGTGGC
2270
PRIMER_RIGHT_11_SEQUENCE=TTACACCCCAATCTTCATGTCC
2271
PRIMER_LEFT_11=28,19
2272
PRIMER_RIGHT_11=1113,22
2273
PRIMER_LEFT_11_TM=58.401
2274
PRIMER_RIGHT_11_TM=60.968
2275
PRIMER_LEFT_11_GC_PERCENT=47.368
2276
PRIMER_RIGHT_11_GC_PERCENT=45.455
2277
PRIMER_LEFT_11_SELF_ANY=3.00
2278
PRIMER_RIGHT_11_SELF_ANY=4.00
2279
PRIMER_LEFT_11_SELF_END=2.00
2280
PRIMER_RIGHT_11_SELF_END=0.00
2281
PRIMER_LEFT_11_POSITION_PENALTY=0.000000
2282
PRIMER_RIGHT_11_POSITION_PENALTY=1.000000
2283
PRIMER_LEFT_11_END_STABILITY=9.4000
2284
PRIMER_RIGHT_11_END_STABILITY=7.9000
2285
PRIMER_PAIR_11_COMPL_ANY=5.00
2286
PRIMER_PAIR_11_COMPL_END=2.00
2287
PRIMER_PRODUCT_SIZE_11=1086
2288
PRIMER_PAIR_PENALTY_12=6.8686
2289
PRIMER_LEFT_12_PENALTY=1.335843
2290
PRIMER_RIGHT_12_PENALTY=5.532744
2291
PRIMER_LEFT_12_SEQUENCE=ATGGACAAGTTTTGGTGGCA
2292
PRIMER_RIGHT_12_SEQUENCE=GTGTTACACCCCAATCTTCATGT
2293
PRIMER_LEFT_12=28,20
2294
PRIMER_RIGHT_12=1116,23
2295
PRIMER_LEFT_12_TM=61.336
2296
PRIMER_RIGHT_12_TM=60.033
2297
PRIMER_LEFT_12_GC_PERCENT=45.000
2298
PRIMER_RIGHT_12_GC_PERCENT=43.478
2299
PRIMER_LEFT_12_SELF_ANY=3.00
2300
PRIMER_RIGHT_12_SELF_ANY=7.00
2301
PRIMER_LEFT_12_SELF_END=3.00
2302
PRIMER_RIGHT_12_SELF_END=2.00
2303
PRIMER_LEFT_12_POSITION_PENALTY=0.000000
2304
PRIMER_RIGHT_12_POSITION_PENALTY=2.500000
2305
PRIMER_LEFT_12_END_STABILITY=10.0000
2306
PRIMER_RIGHT_12_END_STABILITY=6.6000
2307
PRIMER_PAIR_12_COMPL_ANY=5.00
2308
PRIMER_PAIR_12_COMPL_END=2.00
2309
PRIMER_PRODUCT_SIZE_12=1089
2310
PRIMER_PAIR_PENALTY_13=7.2219
2311
PRIMER_LEFT_13_PENALTY=2.598622
2312
PRIMER_RIGHT_13_PENALTY=4.623327
2313
PRIMER_LEFT_13_SEQUENCE=ATGGACAAGTTTTGGTGGC
2314
PRIMER_RIGHT_13_SEQUENCE=TTACACCCCAATCTTCATGTC
2315
PRIMER_LEFT_13=28,19
2316
PRIMER_RIGHT_13=1113,21
2317
PRIMER_LEFT_13_TM=58.401
2318
PRIMER_RIGHT_13_TM=57.377
2319
PRIMER_LEFT_13_GC_PERCENT=47.368
2320
PRIMER_RIGHT_13_GC_PERCENT=42.857
2321
PRIMER_LEFT_13_SELF_ANY=3.00
2322
PRIMER_RIGHT_13_SELF_ANY=4.00
2323
PRIMER_LEFT_13_SELF_END=2.00
2324
PRIMER_RIGHT_13_SELF_END=1.00
2325
PRIMER_LEFT_13_POSITION_PENALTY=0.000000
2326
PRIMER_RIGHT_13_POSITION_PENALTY=1.000000
2327
PRIMER_LEFT_13_END_STABILITY=9.4000
2328
PRIMER_RIGHT_13_END_STABILITY=6.3000
2329
PRIMER_PAIR_13_COMPL_ANY=5.00
2330
PRIMER_PAIR_13_COMPL_END=1.00
2331
PRIMER_PRODUCT_SIZE_13=1086
2332
PRIMER_PAIR_PENALTY_14=7.3461
2333
PRIMER_LEFT_14_PENALTY=1.335843
2334
PRIMER_RIGHT_14_PENALTY=6.010288
2335
PRIMER_LEFT_14_SEQUENCE=ATGGACAAGTTTTGGTGGCA
2336
PRIMER_RIGHT_14_SEQUENCE=TGTTACACCCCAATCTTCATGTC
2337
PRIMER_LEFT_14=28,20
2338
PRIMER_RIGHT_14=1115,23
2339
PRIMER_LEFT_14_TM=61.336
2340
PRIMER_RIGHT_14_TM=61.010
2341
PRIMER_LEFT_14_GC_PERCENT=45.000
2342
PRIMER_RIGHT_14_GC_PERCENT=43.478
2343
PRIMER_LEFT_14_SELF_ANY=3.00
2344
PRIMER_RIGHT_14_SELF_ANY=5.00
2345
PRIMER_LEFT_14_SELF_END=3.00
2346
PRIMER_RIGHT_14_SELF_END=1.00
2347
PRIMER_LEFT_14_POSITION_PENALTY=0.000000
2348
PRIMER_RIGHT_14_POSITION_PENALTY=2.000000
2349
PRIMER_LEFT_14_END_STABILITY=10.0000
2350
PRIMER_RIGHT_14_END_STABILITY=6.3000
2351
PRIMER_PAIR_14_COMPL_ANY=5.00
2352
PRIMER_PAIR_14_COMPL_END=2.00
2353
PRIMER_PRODUCT_SIZE_14=1088
2354
PRIMER_PAIR_PENALTY_15=7.3801
2355
PRIMER_LEFT_15_PENALTY=2.598622
2356
PRIMER_RIGHT_15_PENALTY=4.781508
2357
PRIMER_LEFT_15_SEQUENCE=ATGGACAAGTTTTGGTGGC
2358
PRIMER_RIGHT_15_SEQUENCE=TGTTACACCCCAATCTTCATGT
2359
PRIMER_LEFT_15=28,19
2360
PRIMER_RIGHT_15=1115,22
2361
PRIMER_LEFT_15_TM=58.401
2362
PRIMER_RIGHT_15_TM=59.218
2363
PRIMER_LEFT_15_GC_PERCENT=47.368
2364
PRIMER_RIGHT_15_GC_PERCENT=40.909
2365
PRIMER_LEFT_15_SELF_ANY=3.00
2366
PRIMER_RIGHT_15_SELF_ANY=5.00
2367
PRIMER_LEFT_15_SELF_END=2.00
2368
PRIMER_RIGHT_15_SELF_END=2.00
2369
PRIMER_LEFT_15_POSITION_PENALTY=0.000000
2370
PRIMER_RIGHT_15_POSITION_PENALTY=2.000000
2371
PRIMER_LEFT_15_END_STABILITY=9.4000
2372
PRIMER_RIGHT_15_END_STABILITY=6.6000
2373
PRIMER_PAIR_15_COMPL_ANY=5.00
2374
PRIMER_PAIR_15_COMPL_END=1.00
2375
PRIMER_PRODUCT_SIZE_15=1088
2376
PRIMER_PAIR_PENALTY_16=7.5278
2377
PRIMER_LEFT_16_PENALTY=2.598622
2378
PRIMER_RIGHT_16_PENALTY=4.929154
2379
PRIMER_LEFT_16_SEQUENCE=ATGGACAAGTTTTGGTGGC
2380
PRIMER_RIGHT_16_SEQUENCE=TACACCCCAATCTTCATGTCCA
2381
PRIMER_LEFT_16=28,19
2382
PRIMER_RIGHT_16=1112,22
2383
PRIMER_LEFT_16_TM=58.401
2384
PRIMER_RIGHT_16_TM=62.429
2385
PRIMER_LEFT_16_GC_PERCENT=47.368
2386
PRIMER_RIGHT_16_GC_PERCENT=45.455
2387
PRIMER_LEFT_16_SELF_ANY=3.00
2388
PRIMER_RIGHT_16_SELF_ANY=4.00
2389
PRIMER_LEFT_16_SELF_END=2.00
2390
PRIMER_RIGHT_16_SELF_END=2.00
2391
PRIMER_LEFT_16_POSITION_PENALTY=0.000000
2392
PRIMER_RIGHT_16_POSITION_PENALTY=0.500000
2393
PRIMER_LEFT_16_END_STABILITY=9.4000
2394
PRIMER_RIGHT_16_END_STABILITY=7.9000
2395
PRIMER_PAIR_16_COMPL_ANY=6.00
2396
PRIMER_PAIR_16_COMPL_END=3.00
2397
PRIMER_PRODUCT_SIZE_16=1085
2398
PRIMER_PAIR_PENALTY_17=7.5368
2399
PRIMER_LEFT_17_PENALTY=1.335843
2400
PRIMER_RIGHT_17_PENALTY=6.200997
2401
PRIMER_LEFT_17_SEQUENCE=ATGGACAAGTTTTGGTGGCA
2402
PRIMER_RIGHT_17_SEQUENCE=GTTACACCCCAATCTTCATGTCC
2403
PRIMER_LEFT_17=28,20
2404
PRIMER_RIGHT_17=1114,23
2405
PRIMER_LEFT_17_TM=61.336
2406
PRIMER_RIGHT_17_TM=61.701
2407
PRIMER_LEFT_17_GC_PERCENT=45.000
2408
PRIMER_RIGHT_17_GC_PERCENT=47.826
2409
PRIMER_LEFT_17_SELF_ANY=3.00
2410
PRIMER_RIGHT_17_SELF_ANY=4.00
2411
PRIMER_LEFT_17_SELF_END=3.00
2412
PRIMER_RIGHT_17_SELF_END=0.00
2413
PRIMER_LEFT_17_POSITION_PENALTY=0.000000
2414
PRIMER_RIGHT_17_POSITION_PENALTY=1.500000
2415
PRIMER_LEFT_17_END_STABILITY=10.0000
2416
PRIMER_RIGHT_17_END_STABILITY=7.9000
2417
PRIMER_PAIR_17_COMPL_ANY=5.00
2418
PRIMER_PAIR_17_COMPL_END=2.00
2419
PRIMER_PRODUCT_SIZE_17=1087
2420
PRIMER_PAIR_PENALTY_18=7.8057
2421
PRIMER_LEFT_18_PENALTY=2.598622
2422
PRIMER_RIGHT_18_PENALTY=5.207042
2423
PRIMER_LEFT_18_SEQUENCE=ATGGACAAGTTTTGGTGGC
2424
PRIMER_RIGHT_18_SEQUENCE=GTTACACCCCAATCTTCATGTC
2425
PRIMER_LEFT_18=28,19
2426
PRIMER_RIGHT_18=1114,22
2427
PRIMER_LEFT_18_TM=58.401
2428
PRIMER_RIGHT_18_TM=58.293
2429
PRIMER_LEFT_18_GC_PERCENT=47.368
2430
PRIMER_RIGHT_18_GC_PERCENT=45.455
2431
PRIMER_LEFT_18_SELF_ANY=3.00
2432
PRIMER_RIGHT_18_SELF_ANY=4.00
2433
PRIMER_LEFT_18_SELF_END=2.00
2434
PRIMER_RIGHT_18_SELF_END=1.00
2435
PRIMER_LEFT_18_POSITION_PENALTY=0.000000
2436
PRIMER_RIGHT_18_POSITION_PENALTY=1.500000
2437
PRIMER_LEFT_18_END_STABILITY=9.4000
2438
PRIMER_RIGHT_18_END_STABILITY=6.3000
2439
PRIMER_PAIR_18_COMPL_ANY=5.00
2440
PRIMER_PAIR_18_COMPL_END=1.00
2441
PRIMER_PRODUCT_SIZE_18=1087
2442
PRIMER_PAIR_PENALTY_19=7.8318
2443
PRIMER_LEFT_19_PENALTY=2.598622
2444
PRIMER_RIGHT_19_PENALTY=5.233212
2445
PRIMER_LEFT_19_SEQUENCE=ATGGACAAGTTTTGGTGGC
2446
PRIMER_RIGHT_19_SEQUENCE=ACACCCCAATCTTCATGTCCAC
2447
PRIMER_LEFT_19=28,19
2448
PRIMER_RIGHT_19=1111,22
2449
PRIMER_LEFT_19_TM=58.401
2450
PRIMER_RIGHT_19_TM=63.233
2451
PRIMER_LEFT_19_GC_PERCENT=47.368
2452
PRIMER_RIGHT_19_GC_PERCENT=50.000
2453
PRIMER_LEFT_19_SELF_ANY=3.00
2454
PRIMER_RIGHT_19_SELF_ANY=4.00
2455
PRIMER_LEFT_19_SELF_END=2.00
2456
PRIMER_RIGHT_19_SELF_END=2.00
2457
PRIMER_LEFT_19_POSITION_PENALTY=0.000000
2458
PRIMER_RIGHT_19_POSITION_PENALTY=0.000000
2459
PRIMER_LEFT_19_END_STABILITY=9.4000
2460
PRIMER_RIGHT_19_END_STABILITY=7.9000
2461
PRIMER_PAIR_19_COMPL_ANY=6.00
2462
PRIMER_PAIR_19_COMPL_END=3.00
2463
PRIMER_PRODUCT_SIZE_19=1084
2464
=
2465
PRIMER_SEQUENCE_ID=M67454/FAS
2466
PRIMER_COMMENT=Human Fas antigen (fas) mRNA, complete cds. CDS=195..1202
2467
PRIMER_START_CODON_POSITION=194
2468
SEQUENCE=GACGCTTCTGGGGAGTGAGGGAAGCGGTTTACGAGTGACTTGGCTGGAGCCTCAGGGGCGGGCACTGGCACGGAACACACCCTGAGGCCAGCCCTGGCTGCCCAGGCGGAGCTGCCTCTTCTCCCGCGGGTTGGTGGACCCGCTCAGTACGGAGTTGGGGAAGCTCTTTCACTTCGGAGGATTGCTCAACAACCATGCTGGGCATCTGGACCCTCCTACCTCTGGTTCTTACGTCTGTTGCTAGATTATCGTCCAAAAGTGTTAATGCCCAAGTGACTGACATCAACTCCAAGGGATTGGAATTGAGGAAGACTGTTACTACAGTTGAGACTCAGAACTTGGAAGGCCTGCATCATGATGGCCAATTCTGCCATAAGCCCTGTCCTCCAGGTGAAAGGAAAGCTAGGGACTGCACAGTCAATGGGGATGAACCAGACTGCGTGCCCTGCCAAGAAGGGAAGGAGTACACAGACAAAGCCCATTTTTCTTCCAAATGCAGAAGATGTAGATTGTGTGATGAAGGACATGGCTTAGAAGTGGAAATAAACTGCACCCGGACCCAGAATACCAAGTGCAGATGTAAACCAAACTTTTTTTGTAACTCTACTGTATGTGAACACTGTGACCCTTGCACCAAATGTGAACATGGAATCATCAAGGAATGCACACTCACCAGCAACACCAAGTGCAAAGAGGAAGGATCCAGATCTAACTTGGGGTGGCTTTGTCTTCTTCTTTTGCCAATTCCACTAATTGTTTGGGTGAAGAGAAAGGAAGTACAGAAAACATGCAGAAAGCACAGAAAGGAAAACCAAGGTTCTCATGAATCTCCAACCTTAAATCCTGAAACAGTGGCAATAAATTTATCTGATGTTGACTTGAGTAAATATATCACCACTATTGCTGGAGTCATGACACTAAGTCAAGTTAAAGGCTTTGTTCGAAAGAATGGTGTCAATGAAGCCAAAATAGATGAGATCAAGAATGACAATGTCCAAGACACAGCAGAACAGAAAGTTCAACTGCTTCGTAATTGGCATCAACTTCATGGAAAGAAAGAAGCGTATGACACATTGATTAAAGATCTCAAAAAAGCCAATCTTTGTACTCTTGCAGAGAAAATTCAGACTATCATCCTCAAGGACATTACTAGTGACTCAGAAAATTCAAACTTCAGAAATGAAATCCAAAGCTTGGTCTAGAGTGAAAAACAACAAATTCAGTTCTGAGTATATGCAATTAGTGTTTGAAAAGATTCTTAATAGCTGGCTGTAAATACTGCTTGGTTTTTTACTGGGTACATTTTATCATTTATTAGCGCTGAAGAGCCAACATATTTGTAGATTTTTAATATCTCATGATTCTGCCTCCAAGGATGTTTAAAATCTAGTTGGGAAAACAAACTTCATCAAGAGTAAATGCAGTGGCATGCTAAGTACCCAAATAGGAGTGTATGCAGAGGATGAAAGATTAAGATTATGCTCTGGCATCTAACATATGATTCTGTAGTATGAATGTAATCAGTGTATGTTAGTACAAATGTCTATCCACAGGCTAACCCCACTCTATGAATCAATAGAAGAAGCTATGACCTTTTGCTGAAATATCAGTTACTGAACAGGCAGGCCACTTTGCCTCTAAATTACCTCTGATAATTCTAGAGATTTTACCATATTTCTAAACTTTGTTTATAACTCTGAGAAGATCATATTTATGTAAAGTATATGTATTTGAGTGCAGAATTTAAATAAGGCTCTACCTCAAAGACCTTTGCACAGTTTATTGGTGTCATATTATACAATATTTCAATTGTGAATTCACATAGAAAACATTAAATTATAATGTTTGACTATTATATATGTGTATGCATTTTACTGGCTCAAAACTACCTACTTCTTTCTCAGGCATCAAAAGCATTTTGAGCAGGAGAGTATTACTAGAGCTTTGCCACCTCTCCATTTTTGCCTTGGTGCTCATCTTAATGGCCTAATGCACCCCCAAACATGGAAATATCACCAAAAAATACTTAATAGTCCACCAAAAGGCAAGACTGCCCTTAGAAATTCTAGCCTGGTTTGGAGATACTAACTGCTCTCAGAGAAAGTAGCTTTGTGACATGTCATGAACCCATGTTTGCAATCAAAGATGATAAAATAGATTCTTATTTTTCCCCCACCCCCGAAAATGTTCAATAATGTCCCATGTAAAACCTGCTACAAATGGCAGCTTATACATAGCAATGGTAAAATCATCATCTGGATTTAGGAATTGCTCTTGTCATACCCTCAAGTTTCTAAGATTTAAGATTCTCCTTACTACTATCCTACGTTTAAATATCTTTGAAAGTTTGTATTAAATGTGAATTTTAAGAAATAATATTTATATTTCTGTAAATGTAAACTGTGAAGATAGTTATAAACTGAAGCAGATACCTGGAACCACCTAAAGAACTTCCATTTATGGAGGATTTTTTTGCCCCTTGTGTTTGGAATTATAAAATATAGGTAAAAGTACGTAATTAAATAATGTTTTTG
2469
PRIMER_LEFT_EXPLAIN=considered 21749, would not amplify any of the ORF 18372, GC content failed 10, low tm 984, high tm 1002, high end compl 3, long poly-x seq 44,high 3' stability 21, ok 1313
2470
PRIMER_RIGHT_EXPLAIN=considered 21076, GC content failed 1260, low tm 8619, high tm 3776, long poly-x seq 112,high 3' stability 48, ok 7261
2471
PRIMER_PAIR_EXPLAIN=considered 57, ok 57
2472
PRIMER_STOP_CODON_POSITION=1199
2473
PRIMER_PAIR_PENALTY=124.1313
2474
PRIMER_LEFT_PENALTY=121.524367
2475
PRIMER_RIGHT_PENALTY=2.606973
2476
PRIMER_LEFT_SEQUENCE=GGCATCTGGACCCTCCTAC
2477
PRIMER_RIGHT_SEQUENCE=AGACCAAGCTTTGGATTTCA
2478
PRIMER_LEFT=200,19
2479
PRIMER_RIGHT=1199,20
2480
PRIMER_LEFT_TM=59.476
2481
PRIMER_RIGHT_TM=57.393
2482
PRIMER_LEFT_GC_PERCENT=63.158
2483
PRIMER_RIGHT_GC_PERCENT=40.000
2484
PRIMER_LEFT_SELF_ANY=3.00
2485
PRIMER_RIGHT_SELF_ANY=6.00
2486
PRIMER_LEFT_SELF_END=0.00
2487
PRIMER_RIGHT_SELF_END=3.00
2488
PRIMER_LEFT_POSITION_PENALTY=120.000000
2489
PRIMER_RIGHT_POSITION_PENALTY=0.000000
2490
PRIMER_LEFT_END_STABILITY=6.9000
2491
PRIMER_RIGHT_END_STABILITY=7.3000
2492
PRIMER_PAIR_COMPL_ANY=3.00
2493
PRIMER_PAIR_COMPL_END=0.00
2494
PRIMER_PRODUCT_SIZE=1000
2495
PRIMER_PAIR_PENALTY_1=124.3881
2496
PRIMER_LEFT_1_PENALTY=121.524367
2497
PRIMER_RIGHT_1_PENALTY=2.863756
2498
PRIMER_LEFT_1_SEQUENCE=GGCATCTGGACCCTCCTAC
2499
PRIMER_RIGHT_1_SEQUENCE=AGACCAAGCTTTGGATTTCATT
2500
PRIMER_LEFT_1=200,19
2501
PRIMER_RIGHT_1=1199,22
2502
PRIMER_LEFT_1_TM=59.476
2503
PRIMER_RIGHT_1_TM=59.136
2504
PRIMER_LEFT_1_GC_PERCENT=63.158
2505
PRIMER_RIGHT_1_GC_PERCENT=36.364
2506
PRIMER_LEFT_1_SELF_ANY=3.00
2507
PRIMER_RIGHT_1_SELF_ANY=6.00
2508
PRIMER_LEFT_1_SELF_END=0.00
2509
PRIMER_RIGHT_1_SELF_END=1.00
2510
PRIMER_LEFT_1_POSITION_PENALTY=120.000000
2511
PRIMER_RIGHT_1_POSITION_PENALTY=0.000000
2512
PRIMER_LEFT_1_END_STABILITY=6.9000
2513
PRIMER_RIGHT_1_END_STABILITY=6.9000
2514
PRIMER_PAIR_1_COMPL_ANY=3.00
2515
PRIMER_PAIR_1_COMPL_END=0.00
2516
PRIMER_PRODUCT_SIZE_1=1000
2517
PRIMER_PAIR_PENALTY_2=124.7260
2518
PRIMER_LEFT_2_PENALTY=121.524367
2519
PRIMER_RIGHT_2_PENALTY=3.201621
2520
PRIMER_LEFT_2_SEQUENCE=GGCATCTGGACCCTCCTAC
2521
PRIMER_RIGHT_2_SEQUENCE=AGACCAAGCTTTGGATTTCAT
2522
PRIMER_LEFT_2=200,19
2523
PRIMER_RIGHT_2=1199,21
2524
PRIMER_LEFT_2_TM=59.476
2525
PRIMER_RIGHT_2_TM=57.798
2526
PRIMER_LEFT_2_GC_PERCENT=63.158
2527
PRIMER_RIGHT_2_GC_PERCENT=38.095
2528
PRIMER_LEFT_2_SELF_ANY=3.00
2529
PRIMER_RIGHT_2_SELF_ANY=6.00
2530
PRIMER_LEFT_2_SELF_END=0.00
2531
PRIMER_RIGHT_2_SELF_END=2.00
2532
PRIMER_LEFT_2_POSITION_PENALTY=120.000000
2533
PRIMER_RIGHT_2_POSITION_PENALTY=0.000000
2534
PRIMER_LEFT_2_END_STABILITY=6.9000
2535
PRIMER_RIGHT_2_END_STABILITY=6.9000
2536
PRIMER_PAIR_2_COMPL_ANY=3.00
2537
PRIMER_PAIR_2_COMPL_END=0.00
2538
PRIMER_PRODUCT_SIZE_2=1000
2539
PRIMER_PAIR_PENALTY_3=124.8704
2540
PRIMER_LEFT_3_PENALTY=121.524367
2541
PRIMER_RIGHT_3_PENALTY=3.346072
2542
PRIMER_LEFT_3_SEQUENCE=GGCATCTGGACCCTCCTAC
2543
PRIMER_RIGHT_3_SEQUENCE=AGACCAAGCTTTGGATTTCATTT
2544
PRIMER_LEFT_3=200,19
2545
PRIMER_RIGHT_3=1199,23
2546
PRIMER_LEFT_3_TM=59.476
2547
PRIMER_RIGHT_3_TM=60.346
2548
PRIMER_LEFT_3_GC_PERCENT=63.158
2549
PRIMER_RIGHT_3_GC_PERCENT=34.783
2550
PRIMER_LEFT_3_SELF_ANY=3.00
2551
PRIMER_RIGHT_3_SELF_ANY=6.00
2552
PRIMER_LEFT_3_SELF_END=0.00
2553
PRIMER_RIGHT_3_SELF_END=0.00
2554
PRIMER_LEFT_3_POSITION_PENALTY=120.000000
2555
PRIMER_RIGHT_3_POSITION_PENALTY=0.000000
2556
PRIMER_LEFT_3_END_STABILITY=6.9000
2557
PRIMER_RIGHT_3_END_STABILITY=7.2000
2558
PRIMER_PAIR_3_COMPL_ANY=3.00
2559
PRIMER_PAIR_3_COMPL_END=0.00
2560
PRIMER_PRODUCT_SIZE_3=1000
2561
PRIMER_PAIR_PENALTY_4=125.5220
2562
PRIMER_LEFT_4_PENALTY=121.524367
2563
PRIMER_RIGHT_4_PENALTY=3.997622
2564
PRIMER_LEFT_4_SEQUENCE=GGCATCTGGACCCTCCTAC
2565
PRIMER_RIGHT_4_SEQUENCE=TAGACCAAGCTTTGGATTTCA
2566
PRIMER_LEFT_4=200,19
2567
PRIMER_RIGHT_4=1200,21
2568
PRIMER_LEFT_4_TM=59.476
2569
PRIMER_RIGHT_4_TM=57.502
2570
PRIMER_LEFT_4_GC_PERCENT=63.158
2571
PRIMER_RIGHT_4_GC_PERCENT=38.095
2572
PRIMER_LEFT_4_SELF_ANY=3.00
2573
PRIMER_RIGHT_4_SELF_ANY=6.00
2574
PRIMER_LEFT_4_SELF_END=0.00
2575
PRIMER_RIGHT_4_SELF_END=3.00
2576
PRIMER_LEFT_4_POSITION_PENALTY=120.000000
2577
PRIMER_RIGHT_4_POSITION_PENALTY=0.500000
2578
PRIMER_LEFT_4_END_STABILITY=6.9000
2579
PRIMER_RIGHT_4_END_STABILITY=7.3000
2580
PRIMER_PAIR_4_COMPL_ANY=3.00
2581
PRIMER_PAIR_4_COMPL_END=0.00
2582
PRIMER_PRODUCT_SIZE_4=1001
2583
PRIMER_PAIR_PENALTY_5=125.6899
2584
PRIMER_LEFT_5_PENALTY=123.082915
2585
PRIMER_RIGHT_5_PENALTY=2.606973
2586
PRIMER_LEFT_5_SEQUENCE=GGCATCTGGACCCTCCTACC
2587
PRIMER_RIGHT_5_SEQUENCE=AGACCAAGCTTTGGATTTCA
2588
PRIMER_LEFT_5=200,20
2589
PRIMER_RIGHT_5=1199,20
2590
PRIMER_LEFT_5_TM=63.083
2591
PRIMER_RIGHT_5_TM=57.393
2592
PRIMER_LEFT_5_GC_PERCENT=65.000
2593
PRIMER_RIGHT_5_GC_PERCENT=40.000
2594
PRIMER_LEFT_5_SELF_ANY=3.00
2595
PRIMER_RIGHT_5_SELF_ANY=6.00
2596
PRIMER_LEFT_5_SELF_END=0.00
2597
PRIMER_RIGHT_5_SELF_END=3.00
2598
PRIMER_LEFT_5_POSITION_PENALTY=120.000000
2599
PRIMER_RIGHT_5_POSITION_PENALTY=0.000000
2600
PRIMER_LEFT_5_END_STABILITY=6.9000
2601
PRIMER_RIGHT_5_END_STABILITY=7.3000
2602
PRIMER_PAIR_5_COMPL_ANY=3.00
2603
PRIMER_PAIR_5_COMPL_END=0.00
2604
PRIMER_PRODUCT_SIZE_5=1000
2605
PRIMER_PAIR_PENALTY_6=125.8489
2606
PRIMER_LEFT_6_PENALTY=121.524367
2607
PRIMER_RIGHT_6_PENALTY=4.324579
2608
PRIMER_LEFT_6_SEQUENCE=GGCATCTGGACCCTCCTAC
2609
PRIMER_RIGHT_6_SEQUENCE=TAGACCAAGCTTTGGATTTCATT
2610
PRIMER_LEFT_6=200,19
2611
PRIMER_RIGHT_6=1200,23
2612
PRIMER_LEFT_6_TM=59.476
2613
PRIMER_RIGHT_6_TM=59.175
2614
PRIMER_LEFT_6_GC_PERCENT=63.158
2615
PRIMER_RIGHT_6_GC_PERCENT=34.783
2616
PRIMER_LEFT_6_SELF_ANY=3.00
2617
PRIMER_RIGHT_6_SELF_ANY=6.00
2618
PRIMER_LEFT_6_SELF_END=0.00
2619
PRIMER_RIGHT_6_SELF_END=1.00
2620
PRIMER_LEFT_6_POSITION_PENALTY=120.000000
2621
PRIMER_RIGHT_6_POSITION_PENALTY=0.500000
2622
PRIMER_LEFT_6_END_STABILITY=6.9000
2623
PRIMER_RIGHT_6_END_STABILITY=6.9000
2624
PRIMER_PAIR_6_COMPL_ANY=3.00
2625
PRIMER_PAIR_6_COMPL_END=0.00
2626
PRIMER_PRODUCT_SIZE_6=1001
2627
PRIMER_PAIR_PENALTY_7=125.9467
2628
PRIMER_LEFT_7_PENALTY=123.082915
2629
PRIMER_RIGHT_7_PENALTY=2.863756
2630
PRIMER_LEFT_7_SEQUENCE=GGCATCTGGACCCTCCTACC
2631
PRIMER_RIGHT_7_SEQUENCE=AGACCAAGCTTTGGATTTCATT
2632
PRIMER_LEFT_7=200,20
2633
PRIMER_RIGHT_7=1199,22
2634
PRIMER_LEFT_7_TM=63.083
2635
PRIMER_RIGHT_7_TM=59.136
2636
PRIMER_LEFT_7_GC_PERCENT=65.000
2637
PRIMER_RIGHT_7_GC_PERCENT=36.364
2638
PRIMER_LEFT_7_SELF_ANY=3.00
2639
PRIMER_RIGHT_7_SELF_ANY=6.00
2640
PRIMER_LEFT_7_SELF_END=0.00
2641
PRIMER_RIGHT_7_SELF_END=1.00
2642
PRIMER_LEFT_7_POSITION_PENALTY=120.000000
2643
PRIMER_RIGHT_7_POSITION_PENALTY=0.000000
2644
PRIMER_LEFT_7_END_STABILITY=6.9000
2645
PRIMER_RIGHT_7_END_STABILITY=6.9000
2646
PRIMER_PAIR_7_COMPL_ANY=3.00
2647
PRIMER_PAIR_7_COMPL_END=0.00
2648
PRIMER_PRODUCT_SIZE_7=1000
2649
PRIMER_PAIR_PENALTY_8=126.0095
2650
PRIMER_LEFT_8_PENALTY=121.524367
2651
PRIMER_RIGHT_8_PENALTY=4.485099
2652
PRIMER_LEFT_8_SEQUENCE=GGCATCTGGACCCTCCTAC
2653
PRIMER_RIGHT_8_SEQUENCE=CTAGACCAAGCTTTGGATTTCA
2654
PRIMER_LEFT_8=200,19
2655
PRIMER_RIGHT_8=1201,22
2656
PRIMER_LEFT_8_TM=59.476
2657
PRIMER_RIGHT_8_TM=58.515
2658
PRIMER_LEFT_8_GC_PERCENT=63.158
2659
PRIMER_RIGHT_8_GC_PERCENT=40.909
2660
PRIMER_LEFT_8_SELF_ANY=3.00
2661
PRIMER_RIGHT_8_SELF_ANY=6.00
2662
PRIMER_LEFT_8_SELF_END=0.00
2663
PRIMER_RIGHT_8_SELF_END=3.00
2664
PRIMER_LEFT_8_POSITION_PENALTY=120.000000
2665
PRIMER_RIGHT_8_POSITION_PENALTY=1.000000
2666
PRIMER_LEFT_8_END_STABILITY=6.9000
2667
PRIMER_RIGHT_8_END_STABILITY=7.3000
2668
PRIMER_PAIR_8_COMPL_ANY=3.00
2669
PRIMER_PAIR_8_COMPL_END=0.00
2670
PRIMER_PRODUCT_SIZE_8=1002
2671
PRIMER_PAIR_PENALTY_9=126.1368
2672
PRIMER_LEFT_9_PENALTY=121.524367
2673
PRIMER_RIGHT_9_PENALTY=4.612386
2674
PRIMER_LEFT_9_SEQUENCE=GGCATCTGGACCCTCCTAC
2675
PRIMER_RIGHT_9_SEQUENCE=TAGACCAAGCTTTGGATTTCAT
2676
PRIMER_LEFT_9=200,19
2677
PRIMER_RIGHT_9=1200,22
2678
PRIMER_LEFT_9_TM=59.476
2679
PRIMER_RIGHT_9_TM=57.888
2680
PRIMER_LEFT_9_GC_PERCENT=63.158
2681
PRIMER_RIGHT_9_GC_PERCENT=36.364
2682
PRIMER_LEFT_9_SELF_ANY=3.00
2683
PRIMER_RIGHT_9_SELF_ANY=6.00
2684
PRIMER_LEFT_9_SELF_END=0.00
2685
PRIMER_RIGHT_9_SELF_END=2.00
2686
PRIMER_LEFT_9_POSITION_PENALTY=120.000000
2687
PRIMER_RIGHT_9_POSITION_PENALTY=0.500000
2688
PRIMER_LEFT_9_END_STABILITY=6.9000
2689
PRIMER_RIGHT_9_END_STABILITY=6.9000
2690
PRIMER_PAIR_9_COMPL_ANY=3.00
2691
PRIMER_PAIR_9_COMPL_END=0.00
2692
PRIMER_PRODUCT_SIZE_9=1001
2693
PRIMER_PAIR_PENALTY_10=126.2673
2694
PRIMER_LEFT_10_PENALTY=121.524367
2695
PRIMER_RIGHT_10_PENALTY=4.742983
2696
PRIMER_LEFT_10_SEQUENCE=GGCATCTGGACCCTCCTAC
2697
PRIMER_RIGHT_10_SEQUENCE=TCTAGACCAAGCTTTGGATTTCA
2698
PRIMER_LEFT_10=200,19
2699
PRIMER_RIGHT_10=1202,23
2700
PRIMER_LEFT_10_TM=59.476
2701
PRIMER_RIGHT_10_TM=60.243
2702
PRIMER_LEFT_10_GC_PERCENT=63.158
2703
PRIMER_RIGHT_10_GC_PERCENT=39.130
2704
PRIMER_LEFT_10_SELF_ANY=3.00
2705
PRIMER_RIGHT_10_SELF_ANY=6.00
2706
PRIMER_LEFT_10_SELF_END=0.00
2707
PRIMER_RIGHT_10_SELF_END=3.00
2708
PRIMER_LEFT_10_POSITION_PENALTY=120.000000
2709
PRIMER_RIGHT_10_POSITION_PENALTY=1.500000
2710
PRIMER_LEFT_10_END_STABILITY=6.9000
2711
PRIMER_RIGHT_10_END_STABILITY=7.3000
2712
PRIMER_PAIR_10_COMPL_ANY=4.00
2713
PRIMER_PAIR_10_COMPL_END=0.00
2714
PRIMER_PRODUCT_SIZE_10=1003
2715
PRIMER_PAIR_PENALTY_11=126.2845
2716
PRIMER_LEFT_11_PENALTY=123.082915
2717
PRIMER_RIGHT_11_PENALTY=3.201621
2718
PRIMER_LEFT_11_SEQUENCE=GGCATCTGGACCCTCCTACC
2719
PRIMER_RIGHT_11_SEQUENCE=AGACCAAGCTTTGGATTTCAT
2720
PRIMER_LEFT_11=200,20
2721
PRIMER_RIGHT_11=1199,21
2722
PRIMER_LEFT_11_TM=63.083
2723
PRIMER_RIGHT_11_TM=57.798
2724
PRIMER_LEFT_11_GC_PERCENT=65.000
2725
PRIMER_RIGHT_11_GC_PERCENT=38.095
2726
PRIMER_LEFT_11_SELF_ANY=3.00
2727
PRIMER_RIGHT_11_SELF_ANY=6.00
2728
PRIMER_LEFT_11_SELF_END=0.00
2729
PRIMER_RIGHT_11_SELF_END=2.00
2730
PRIMER_LEFT_11_POSITION_PENALTY=120.000000
2731
PRIMER_RIGHT_11_POSITION_PENALTY=0.000000
2732
PRIMER_LEFT_11_END_STABILITY=6.9000
2733
PRIMER_RIGHT_11_END_STABILITY=6.9000
2734
PRIMER_PAIR_11_COMPL_ANY=3.00
2735
PRIMER_PAIR_11_COMPL_END=0.00
2736
PRIMER_PRODUCT_SIZE_11=1000
2737
PRIMER_PAIR_PENALTY_12=126.3684
2738
PRIMER_LEFT_12_PENALTY=121.524367
2739
PRIMER_RIGHT_12_PENALTY=4.843999
2740
PRIMER_LEFT_12_SEQUENCE=GGCATCTGGACCCTCCTAC
2741
PRIMER_RIGHT_12_SEQUENCE=TAGACCAAGCTTTGGATTTCATTT
2742
PRIMER_LEFT_12=200,19
2743
PRIMER_RIGHT_12=1200,24
2744
PRIMER_LEFT_12_TM=59.476
2745
PRIMER_RIGHT_12_TM=60.344
2746
PRIMER_LEFT_12_GC_PERCENT=63.158
2747
PRIMER_RIGHT_12_GC_PERCENT=33.333
2748
PRIMER_LEFT_12_SELF_ANY=3.00
2749
PRIMER_RIGHT_12_SELF_ANY=6.00
2750
PRIMER_LEFT_12_SELF_END=0.00
2751
PRIMER_RIGHT_12_SELF_END=0.00
2752
PRIMER_LEFT_12_POSITION_PENALTY=120.000000
2753
PRIMER_RIGHT_12_POSITION_PENALTY=0.500000
2754
PRIMER_LEFT_12_END_STABILITY=6.9000
2755
PRIMER_RIGHT_12_END_STABILITY=7.2000
2756
PRIMER_PAIR_12_COMPL_ANY=3.00
2757
PRIMER_PAIR_12_COMPL_END=0.00
2758
PRIMER_PRODUCT_SIZE_12=1001
2759
PRIMER_PAIR_PENALTY_13=126.4290
2760
PRIMER_LEFT_13_PENALTY=123.082915
2761
PRIMER_RIGHT_13_PENALTY=3.346072
2762
PRIMER_LEFT_13_SEQUENCE=GGCATCTGGACCCTCCTACC
2763
PRIMER_RIGHT_13_SEQUENCE=AGACCAAGCTTTGGATTTCATTT
2764
PRIMER_LEFT_13=200,20
2765
PRIMER_RIGHT_13=1199,23
2766
PRIMER_LEFT_13_TM=63.083
2767
PRIMER_RIGHT_13_TM=60.346
2768
PRIMER_LEFT_13_GC_PERCENT=65.000
2769
PRIMER_RIGHT_13_GC_PERCENT=34.783
2770
PRIMER_LEFT_13_SELF_ANY=3.00
2771
PRIMER_RIGHT_13_SELF_ANY=6.00
2772
PRIMER_LEFT_13_SELF_END=0.00
2773
PRIMER_RIGHT_13_SELF_END=0.00
2774
PRIMER_LEFT_13_POSITION_PENALTY=120.000000
2775
PRIMER_RIGHT_13_POSITION_PENALTY=0.000000
2776
PRIMER_LEFT_13_END_STABILITY=6.9000
2777
PRIMER_RIGHT_13_END_STABILITY=7.2000
2778
PRIMER_PAIR_13_COMPL_ANY=3.00
2779
PRIMER_PAIR_13_COMPL_END=0.00
2780
PRIMER_PRODUCT_SIZE_13=1000
2781
PRIMER_PAIR_PENALTY_14=126.5506
2782
PRIMER_LEFT_14_PENALTY=121.524367
2783
PRIMER_RIGHT_14_PENALTY=5.026278
2784
PRIMER_LEFT_14_SEQUENCE=GGCATCTGGACCCTCCTAC
2785
PRIMER_RIGHT_14_SEQUENCE=CTAGACCAAGCTTTGGATTTCATT
2786
PRIMER_LEFT_14=200,19
2787
PRIMER_RIGHT_14=1201,24
2788
PRIMER_LEFT_14_TM=59.476
2789
PRIMER_RIGHT_14_TM=60.026
2790
PRIMER_LEFT_14_GC_PERCENT=63.158
2791
PRIMER_RIGHT_14_GC_PERCENT=37.500
2792
PRIMER_LEFT_14_SELF_ANY=3.00
2793
PRIMER_RIGHT_14_SELF_ANY=6.00
2794
PRIMER_LEFT_14_SELF_END=0.00
2795
PRIMER_RIGHT_14_SELF_END=1.00
2796
PRIMER_LEFT_14_POSITION_PENALTY=120.000000
2797
PRIMER_RIGHT_14_POSITION_PENALTY=1.000000
2798
PRIMER_LEFT_14_END_STABILITY=6.9000
2799
PRIMER_RIGHT_14_END_STABILITY=6.9000
2800
PRIMER_PAIR_14_COMPL_ANY=3.00
2801
PRIMER_PAIR_14_COMPL_END=0.00
2802
PRIMER_PRODUCT_SIZE_14=1002
2803
PRIMER_PAIR_PENALTY_15=126.6898
2804
PRIMER_LEFT_15_PENALTY=121.524367
2805
PRIMER_RIGHT_15_PENALTY=5.165434
2806
PRIMER_LEFT_15_SEQUENCE=GGCATCTGGACCCTCCTAC
2807
PRIMER_RIGHT_15_SEQUENCE=CTAGACCAAGCTTTGGATTTCAT
2808
PRIMER_LEFT_15=200,19
2809
PRIMER_RIGHT_15=1201,23
2810
PRIMER_LEFT_15_TM=59.476
2811
PRIMER_RIGHT_15_TM=58.835
2812
PRIMER_LEFT_15_GC_PERCENT=63.158
2813
PRIMER_RIGHT_15_GC_PERCENT=39.130
2814
PRIMER_LEFT_15_SELF_ANY=3.00
2815
PRIMER_RIGHT_15_SELF_ANY=6.00
2816
PRIMER_LEFT_15_SELF_END=0.00
2817
PRIMER_RIGHT_15_SELF_END=2.00
2818
PRIMER_LEFT_15_POSITION_PENALTY=120.000000
2819
PRIMER_RIGHT_15_POSITION_PENALTY=1.000000
2820
PRIMER_LEFT_15_END_STABILITY=6.9000
2821
PRIMER_RIGHT_15_END_STABILITY=6.9000
2822
PRIMER_PAIR_15_COMPL_ANY=3.00
2823
PRIMER_PAIR_15_COMPL_END=0.00
2824
PRIMER_PRODUCT_SIZE_15=1002
2825
PRIMER_PAIR_PENALTY_16=127.0805
2826
PRIMER_LEFT_16_PENALTY=123.082915
2827
PRIMER_RIGHT_16_PENALTY=3.997622
2828
PRIMER_LEFT_16_SEQUENCE=GGCATCTGGACCCTCCTACC
2829
PRIMER_RIGHT_16_SEQUENCE=TAGACCAAGCTTTGGATTTCA
2830
PRIMER_LEFT_16=200,20
2831
PRIMER_RIGHT_16=1200,21
2832
PRIMER_LEFT_16_TM=63.083
2833
PRIMER_RIGHT_16_TM=57.502
2834
PRIMER_LEFT_16_GC_PERCENT=65.000
2835
PRIMER_RIGHT_16_GC_PERCENT=38.095
2836
PRIMER_LEFT_16_SELF_ANY=3.00
2837
PRIMER_RIGHT_16_SELF_ANY=6.00
2838
PRIMER_LEFT_16_SELF_END=0.00
2839
PRIMER_RIGHT_16_SELF_END=3.00
2840
PRIMER_LEFT_16_POSITION_PENALTY=120.000000
2841
PRIMER_RIGHT_16_POSITION_PENALTY=0.500000
2842
PRIMER_LEFT_16_END_STABILITY=6.9000
2843
PRIMER_RIGHT_16_END_STABILITY=7.3000
2844
PRIMER_PAIR_16_COMPL_ANY=3.00
2845
PRIMER_PAIR_16_COMPL_END=0.00
2846
PRIMER_PRODUCT_SIZE_16=1001
2847
PRIMER_PAIR_PENALTY_17=127.3898
2848
PRIMER_LEFT_17_PENALTY=121.524367
2849
PRIMER_RIGHT_17_PENALTY=5.865428
2850
PRIMER_LEFT_17_SEQUENCE=GGCATCTGGACCCTCCTAC
2851
PRIMER_RIGHT_17_SEQUENCE=TCTAGACCAAGCTTTGGATTTC
2852
PRIMER_LEFT_17=200,19
2853
PRIMER_RIGHT_17=1202,22
2854
PRIMER_LEFT_17_TM=59.476
2855
PRIMER_RIGHT_17_TM=57.635
2856
PRIMER_LEFT_17_GC_PERCENT=63.158
2857
PRIMER_RIGHT_17_GC_PERCENT=40.909
2858
PRIMER_LEFT_17_SELF_ANY=3.00
2859
PRIMER_RIGHT_17_SELF_ANY=6.00
2860
PRIMER_LEFT_17_SELF_END=0.00
2861
PRIMER_RIGHT_17_SELF_END=2.00
2862
PRIMER_LEFT_17_POSITION_PENALTY=120.000000
2863
PRIMER_RIGHT_17_POSITION_PENALTY=1.500000
2864
PRIMER_LEFT_17_END_STABILITY=6.9000
2865
PRIMER_RIGHT_17_END_STABILITY=6.9000
2866
PRIMER_PAIR_17_COMPL_ANY=4.00
2867
PRIMER_PAIR_17_COMPL_END=0.00
2868
PRIMER_PRODUCT_SIZE_17=1003
2869
PRIMER_PAIR_PENALTY_18=127.4075
2870
PRIMER_LEFT_18_PENALTY=123.082915
2871
PRIMER_RIGHT_18_PENALTY=4.324579
2872
PRIMER_LEFT_18_SEQUENCE=GGCATCTGGACCCTCCTACC
2873
PRIMER_RIGHT_18_SEQUENCE=TAGACCAAGCTTTGGATTTCATT
2874
PRIMER_LEFT_18=200,20
2875
PRIMER_RIGHT_18=1200,23
2876
PRIMER_LEFT_18_TM=63.083
2877
PRIMER_RIGHT_18_TM=59.175
2878
PRIMER_LEFT_18_GC_PERCENT=65.000
2879
PRIMER_RIGHT_18_GC_PERCENT=34.783
2880
PRIMER_LEFT_18_SELF_ANY=3.00
2881
PRIMER_RIGHT_18_SELF_ANY=6.00
2882
PRIMER_LEFT_18_SELF_END=0.00
2883
PRIMER_RIGHT_18_SELF_END=1.00
2884
PRIMER_LEFT_18_POSITION_PENALTY=120.000000
2885
PRIMER_RIGHT_18_POSITION_PENALTY=0.500000
2886
PRIMER_LEFT_18_END_STABILITY=6.9000
2887
PRIMER_RIGHT_18_END_STABILITY=6.9000
2888
PRIMER_PAIR_18_COMPL_ANY=3.00
2889
PRIMER_PAIR_18_COMPL_END=0.00
2890
PRIMER_PRODUCT_SIZE_18=1001
2891
PRIMER_PAIR_PENALTY_19=127.4358
2892
PRIMER_LEFT_19_PENALTY=121.524367
2893
PRIMER_RIGHT_19_PENALTY=5.911413
2894
PRIMER_LEFT_19_SEQUENCE=GGCATCTGGACCCTCCTAC
2895
PRIMER_RIGHT_19_SEQUENCE=AGACCAAGCTTTGGATTTCATTTC
2896
PRIMER_LEFT_19=200,19
2897
PRIMER_RIGHT_19=1199,24
2898
PRIMER_LEFT_19_TM=59.476
2899
PRIMER_RIGHT_19_TM=61.911
2900
PRIMER_LEFT_19_GC_PERCENT=63.158
2901
PRIMER_RIGHT_19_GC_PERCENT=37.500
2902
PRIMER_LEFT_19_SELF_ANY=3.00
2903
PRIMER_RIGHT_19_SELF_ANY=6.00
2904
PRIMER_LEFT_19_SELF_END=0.00
2905
PRIMER_RIGHT_19_SELF_END=1.00
2906
PRIMER_LEFT_19_POSITION_PENALTY=120.000000
2907
PRIMER_RIGHT_19_POSITION_PENALTY=0.000000
2908
PRIMER_LEFT_19_END_STABILITY=6.9000
2909
PRIMER_RIGHT_19_END_STABILITY=6.9000
2910
PRIMER_PAIR_19_COMPL_ANY=3.00
2911
PRIMER_PAIR_19_COMPL_END=0.00
2912
PRIMER_PRODUCT_SIZE_19=1000
2913
=
2914
PRIMER_SEQUENCE_ID=U33760/SKP1
2915
PRIMER_COMMENT=Human cyclin A/CDK2-associated p19 (Skp1) mRNA, complete cds. CDS=94..585
2916
PRIMER_START_CODON_POSITION=93
2917
SEQUENCE=AGGATCCTCGAGGCCACGAAGGCCCTCTCCCTTCGCAAACGCCTCCCGGCTCTCGTAAGCCTCCCGCCGGCCGTCTCCTTAACACCGAACACCATGCCTTCAATTAAGTTGCAGAGTTCTGATGGAGAGATATTTGAAGTTGATGTGGAAATTGCCAAACAATCTGTAACTATTAAGACCATGTTGGAAGATTTGGGAATGGATGATGAAGGAGATGATGACCCAGTTCCTCTACCAAATGTGAATGCAGCAATATTAAAAAAGGTCATTCAGTGGTGCACCCACCACAAGGATGACCCTCCTCCTCCTGAAGATGATGAGAACAAAGAAAAGCGGACAGATGATATCCCTGTTTGGGACCAAGAATTCCTGAAAGTTGACCAAGGAACACTTTTTGAACTCATTCTGGCTGCAAACTACTTAGACATCAAAGGTTTGCTTGATGTTACATGCAAGACTGTTGCCAATATGATCAAGGGGAAAACTCCTGAGGAGATTCGCAAGACCTTCAATATCAAAAATGACTTTACTGAAGAGGAGGAAGCCCAGGTACGCAAAGAGAACCAGTGGTGTGAAGAGAAGTGAAATGTTGTGCCTGACACTGTAACACTGTAAGGAT
2918
PRIMER_LEFT_EXPLAIN=considered 4706, would not amplify any of the ORF 3123, GC content failed 5, low tm 396, high tm 523, high end compl 19, long poly-x seq 20,high 3' stability 13, ok 607
2919
PRIMER_RIGHT_EXPLAIN=considered 4645, GC content failed 11, low tm 1320, high tm 1167, high end compl 10, long poly-x seq 22,high 3' stability 16, ok 2099
2920
PRIMER_PAIR_EXPLAIN=considered 99, high end compl 45, ok 54
2921
PRIMER_STOP_CODON_POSITION=582
2922
PRIMER_PAIR_PENALTY=12.3522
2923
PRIMER_LEFT_PENALTY=9.051374
2924
PRIMER_RIGHT_PENALTY=3.300825
2925
PRIMER_LEFT_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
2926
PRIMER_RIGHT_SEQUENCE=ACTTCTCTTCACACCACTGGTTC
2927
PRIMER_LEFT=93,26
2928
PRIMER_RIGHT=582,23
2929
PRIMER_LEFT_TM=63.051
2930
PRIMER_RIGHT_TM=59.699
2931
PRIMER_LEFT_GC_PERCENT=38.462
2932
PRIMER_RIGHT_GC_PERCENT=47.826
2933
PRIMER_LEFT_SELF_ANY=8.00
2934
PRIMER_RIGHT_SELF_ANY=7.00
2935
PRIMER_LEFT_SELF_END=2.00
2936
PRIMER_RIGHT_SELF_END=3.00
2937
PRIMER_LEFT_POSITION_PENALTY=0.000000
2938
PRIMER_RIGHT_POSITION_PENALTY=0.000000
2939
PRIMER_LEFT_END_STABILITY=6.4000
2940
PRIMER_RIGHT_END_STABILITY=7.9000
2941
PRIMER_PAIR_COMPL_ANY=5.00
2942
PRIMER_PAIR_COMPL_END=0.00
2943
PRIMER_PRODUCT_SIZE=490
2944
PRIMER_PAIR_PENALTY_1=13.6635
2945
PRIMER_LEFT_1_PENALTY=9.051374
2946
PRIMER_RIGHT_1_PENALTY=4.612170
2947
PRIMER_LEFT_1_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
2948
PRIMER_RIGHT_1_SEQUENCE=ACTTCTCTTCACACCACTGGTTCT
2949
PRIMER_LEFT_1=93,26
2950
PRIMER_RIGHT_1=582,24
2951
PRIMER_LEFT_1_TM=63.051
2952
PRIMER_RIGHT_1_TM=60.612
2953
PRIMER_LEFT_1_GC_PERCENT=38.462
2954
PRIMER_RIGHT_1_GC_PERCENT=45.833
2955
PRIMER_LEFT_1_SELF_ANY=8.00
2956
PRIMER_RIGHT_1_SELF_ANY=7.00
2957
PRIMER_LEFT_1_SELF_END=2.00
2958
PRIMER_RIGHT_1_SELF_END=3.00
2959
PRIMER_LEFT_1_POSITION_PENALTY=0.000000
2960
PRIMER_RIGHT_1_POSITION_PENALTY=0.000000
2961
PRIMER_LEFT_1_END_STABILITY=6.4000
2962
PRIMER_RIGHT_1_END_STABILITY=6.4000
2963
PRIMER_PAIR_1_COMPL_ANY=5.00
2964
PRIMER_PAIR_1_COMPL_END=1.00
2965
PRIMER_PRODUCT_SIZE_1=490
2966
PRIMER_PAIR_PENALTY_2=14.5623
2967
PRIMER_LEFT_2_PENALTY=9.051374
2968
PRIMER_RIGHT_2_PENALTY=5.510876
2969
PRIMER_LEFT_2_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
2970
PRIMER_RIGHT_2_SEQUENCE=TTCACTTCTCTTCACACCACTG
2971
PRIMER_LEFT_2=93,26
2972
PRIMER_RIGHT_2=585,22
2973
PRIMER_LEFT_2_TM=63.051
2974
PRIMER_RIGHT_2_TM=57.989
2975
PRIMER_LEFT_2_GC_PERCENT=38.462
2976
PRIMER_RIGHT_2_GC_PERCENT=45.455
2977
PRIMER_LEFT_2_SELF_ANY=8.00
2978
PRIMER_RIGHT_2_SELF_ANY=3.00
2979
PRIMER_LEFT_2_SELF_END=2.00
2980
PRIMER_RIGHT_2_SELF_END=3.00
2981
PRIMER_LEFT_2_POSITION_PENALTY=0.000000
2982
PRIMER_RIGHT_2_POSITION_PENALTY=1.500000
2983
PRIMER_LEFT_2_END_STABILITY=6.4000
2984
PRIMER_RIGHT_2_END_STABILITY=6.7000
2985
PRIMER_PAIR_2_COMPL_ANY=5.00
2986
PRIMER_PAIR_2_COMPL_END=2.00
2987
PRIMER_PRODUCT_SIZE_2=493
2988
PRIMER_PAIR_PENALTY_3=14.6221
2989
PRIMER_LEFT_3_PENALTY=9.051374
2990
PRIMER_RIGHT_3_PENALTY=5.570716
2991
PRIMER_LEFT_3_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
2992
PRIMER_RIGHT_3_SEQUENCE=TTTCACTTCTCTTCACACCACTG
2993
PRIMER_LEFT_3=93,26
2994
PRIMER_RIGHT_3=586,23
2995
PRIMER_LEFT_3_TM=63.051
2996
PRIMER_RIGHT_3_TM=59.429
2997
PRIMER_LEFT_3_GC_PERCENT=38.462
2998
PRIMER_RIGHT_3_GC_PERCENT=43.478
2999
PRIMER_LEFT_3_SELF_ANY=8.00
3000
PRIMER_RIGHT_3_SELF_ANY=3.00
3001
PRIMER_LEFT_3_SELF_END=2.00
3002
PRIMER_RIGHT_3_SELF_END=3.00
3003
PRIMER_LEFT_3_POSITION_PENALTY=0.000000
3004
PRIMER_RIGHT_3_POSITION_PENALTY=2.000000
3005
PRIMER_LEFT_3_END_STABILITY=6.4000
3006
PRIMER_RIGHT_3_END_STABILITY=6.7000
3007
PRIMER_PAIR_3_COMPL_ANY=5.00
3008
PRIMER_PAIR_3_COMPL_END=2.00
3009
PRIMER_PRODUCT_SIZE_3=494
3010
PRIMER_PAIR_PENALTY_4=15.8310
3011
PRIMER_LEFT_4_PENALTY=9.051374
3012
PRIMER_RIGHT_4_PENALTY=6.779595
3013
PRIMER_LEFT_4_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
3014
PRIMER_RIGHT_4_SEQUENCE=ATTTCACTTCTCTTCACACCACTG
3015
PRIMER_LEFT_4=93,26
3016
PRIMER_RIGHT_4=587,24
3017
PRIMER_LEFT_4_TM=63.051
3018
PRIMER_RIGHT_4_TM=59.720
3019
PRIMER_LEFT_4_GC_PERCENT=38.462
3020
PRIMER_RIGHT_4_GC_PERCENT=41.667
3021
PRIMER_LEFT_4_SELF_ANY=8.00
3022
PRIMER_RIGHT_4_SELF_ANY=3.00
3023
PRIMER_LEFT_4_SELF_END=2.00
3024
PRIMER_RIGHT_4_SELF_END=3.00
3025
PRIMER_LEFT_4_POSITION_PENALTY=0.000000
3026
PRIMER_RIGHT_4_POSITION_PENALTY=2.500000
3027
PRIMER_LEFT_4_END_STABILITY=6.4000
3028
PRIMER_RIGHT_4_END_STABILITY=6.7000
3029
PRIMER_PAIR_4_COMPL_ANY=5.00
3030
PRIMER_PAIR_4_COMPL_END=2.00
3031
PRIMER_PRODUCT_SIZE_4=495
3032
PRIMER_PAIR_PENALTY_5=15.9173
3033
PRIMER_LEFT_5_PENALTY=9.051374
3034
PRIMER_RIGHT_5_PENALTY=6.865886
3035
PRIMER_LEFT_5_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
3036
PRIMER_RIGHT_5_SEQUENCE=CACTTCTCTTCACACCACTGGTTC
3037
PRIMER_LEFT_5=93,26
3038
PRIMER_RIGHT_5=583,24
3039
PRIMER_LEFT_5_TM=63.051
3040
PRIMER_RIGHT_5_TM=62.366
3041
PRIMER_LEFT_5_GC_PERCENT=38.462
3042
PRIMER_RIGHT_5_GC_PERCENT=50.000
3043
PRIMER_LEFT_5_SELF_ANY=8.00
3044
PRIMER_RIGHT_5_SELF_ANY=7.00
3045
PRIMER_LEFT_5_SELF_END=2.00
3046
PRIMER_RIGHT_5_SELF_END=3.00
3047
PRIMER_LEFT_5_POSITION_PENALTY=0.000000
3048
PRIMER_RIGHT_5_POSITION_PENALTY=0.500000
3049
PRIMER_LEFT_5_END_STABILITY=6.4000
3050
PRIMER_RIGHT_5_END_STABILITY=7.9000
3051
PRIMER_PAIR_5_COMPL_ANY=5.00
3052
PRIMER_PAIR_5_COMPL_END=0.00
3053
PRIMER_PRODUCT_SIZE_5=491
3054
PRIMER_PAIR_PENALTY_6=16.1955
3055
PRIMER_LEFT_6_PENALTY=9.051374
3056
PRIMER_RIGHT_6_PENALTY=7.144164
3057
PRIMER_LEFT_6_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
3058
PRIMER_RIGHT_6_SEQUENCE=CATTTCACTTCTCTTCACACCA
3059
PRIMER_LEFT_6=93,26
3060
PRIMER_RIGHT_6=588,22
3061
PRIMER_LEFT_6_TM=63.051
3062
PRIMER_RIGHT_6_TM=57.856
3063
PRIMER_LEFT_6_GC_PERCENT=38.462
3064
PRIMER_RIGHT_6_GC_PERCENT=40.909
3065
PRIMER_LEFT_6_SELF_ANY=8.00
3066
PRIMER_RIGHT_6_SELF_ANY=2.00
3067
PRIMER_LEFT_6_SELF_END=2.00
3068
PRIMER_RIGHT_6_SELF_END=0.00
3069
PRIMER_LEFT_6_POSITION_PENALTY=0.000000
3070
PRIMER_RIGHT_6_POSITION_PENALTY=3.000000
3071
PRIMER_LEFT_6_END_STABILITY=6.4000
3072
PRIMER_RIGHT_6_END_STABILITY=8.2000
3073
PRIMER_PAIR_6_COMPL_ANY=5.00
3074
PRIMER_PAIR_6_COMPL_END=0.00
3075
PRIMER_PRODUCT_SIZE_6=496
3076
PRIMER_PAIR_PENALTY_7=16.2938
3077
PRIMER_LEFT_7_PENALTY=9.051374
3078
PRIMER_RIGHT_7_PENALTY=7.242436
3079
PRIMER_LEFT_7_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
3080
PRIMER_RIGHT_7_SEQUENCE=CATTTCACTTCTCTTCACACCAC
3081
PRIMER_LEFT_7=93,26
3082
PRIMER_RIGHT_7=588,23
3083
PRIMER_LEFT_7_TM=63.051
3084
PRIMER_RIGHT_7_TM=58.758
3085
PRIMER_LEFT_7_GC_PERCENT=38.462
3086
PRIMER_RIGHT_7_GC_PERCENT=43.478
3087
PRIMER_LEFT_7_SELF_ANY=8.00
3088
PRIMER_RIGHT_7_SELF_ANY=2.00
3089
PRIMER_LEFT_7_SELF_END=2.00
3090
PRIMER_RIGHT_7_SELF_END=0.00
3091
PRIMER_LEFT_7_POSITION_PENALTY=0.000000
3092
PRIMER_RIGHT_7_POSITION_PENALTY=3.000000
3093
PRIMER_LEFT_7_END_STABILITY=6.4000
3094
PRIMER_RIGHT_7_END_STABILITY=7.6000
3095
PRIMER_PAIR_7_COMPL_ANY=5.00
3096
PRIMER_PAIR_7_COMPL_END=0.00
3097
PRIMER_PRODUCT_SIZE_7=496
3098
PRIMER_PAIR_PENALTY_8=16.3296
3099
PRIMER_LEFT_8_PENALTY=9.051374
3100
PRIMER_RIGHT_8_PENALTY=7.278253
3101
PRIMER_LEFT_8_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
3102
PRIMER_RIGHT_8_SEQUENCE=ACTTCTCTTCACACCACTGGTTCTC
3103
PRIMER_LEFT_8=93,26
3104
PRIMER_RIGHT_8=582,25
3105
PRIMER_LEFT_8_TM=63.051
3106
PRIMER_RIGHT_8_TM=62.278
3107
PRIMER_LEFT_8_GC_PERCENT=38.462
3108
PRIMER_RIGHT_8_GC_PERCENT=48.000
3109
PRIMER_LEFT_8_SELF_ANY=8.00
3110
PRIMER_RIGHT_8_SELF_ANY=7.00
3111
PRIMER_LEFT_8_SELF_END=2.00
3112
PRIMER_RIGHT_8_SELF_END=1.00
3113
PRIMER_LEFT_8_POSITION_PENALTY=0.000000
3114
PRIMER_RIGHT_8_POSITION_PENALTY=0.000000
3115
PRIMER_LEFT_8_END_STABILITY=6.4000
3116
PRIMER_RIGHT_8_END_STABILITY=6.7000
3117
PRIMER_PAIR_8_COMPL_ANY=5.00
3118
PRIMER_PAIR_8_COMPL_END=2.00
3119
PRIMER_PRODUCT_SIZE_8=490
3120
PRIMER_PAIR_PENALTY_9=16.3310
3121
PRIMER_LEFT_9_PENALTY=9.051374
3122
PRIMER_RIGHT_9_PENALTY=7.279595
3123
PRIMER_LEFT_9_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
3124
PRIMER_RIGHT_9_SEQUENCE=CATTTCACTTCTCTTCACACCACT
3125
PRIMER_LEFT_9=93,26
3126
PRIMER_RIGHT_9=588,24
3127
PRIMER_LEFT_9_TM=63.051
3128
PRIMER_RIGHT_9_TM=59.720
3129
PRIMER_LEFT_9_GC_PERCENT=38.462
3130
PRIMER_RIGHT_9_GC_PERCENT=41.667
3131
PRIMER_LEFT_9_SELF_ANY=8.00
3132
PRIMER_RIGHT_9_SELF_ANY=2.00
3133
PRIMER_LEFT_9_SELF_END=2.00
3134
PRIMER_RIGHT_9_SELF_END=1.00
3135
PRIMER_LEFT_9_POSITION_PENALTY=0.000000
3136
PRIMER_RIGHT_9_POSITION_PENALTY=3.000000
3137
PRIMER_LEFT_9_END_STABILITY=6.4000
3138
PRIMER_RIGHT_9_END_STABILITY=7.9000
3139
PRIMER_PAIR_9_COMPL_ANY=5.00
3140
PRIMER_PAIR_9_COMPL_END=1.00
3141
PRIMER_PRODUCT_SIZE_9=496
3142
PRIMER_PAIR_PENALTY_10=16.7938
3143
PRIMER_LEFT_10_PENALTY=9.051374
3144
PRIMER_RIGHT_10_PENALTY=7.742436
3145
PRIMER_LEFT_10_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
3146
PRIMER_RIGHT_10_SEQUENCE=ACATTTCACTTCTCTTCACACCA
3147
PRIMER_LEFT_10=93,26
3148
PRIMER_RIGHT_10=589,23
3149
PRIMER_LEFT_10_TM=63.051
3150
PRIMER_RIGHT_10_TM=58.758
3151
PRIMER_LEFT_10_GC_PERCENT=38.462
3152
PRIMER_RIGHT_10_GC_PERCENT=39.130
3153
PRIMER_LEFT_10_SELF_ANY=8.00
3154
PRIMER_RIGHT_10_SELF_ANY=2.00
3155
PRIMER_LEFT_10_SELF_END=2.00
3156
PRIMER_RIGHT_10_SELF_END=0.00
3157
PRIMER_LEFT_10_POSITION_PENALTY=0.000000
3158
PRIMER_RIGHT_10_POSITION_PENALTY=3.500000
3159
PRIMER_LEFT_10_END_STABILITY=6.4000
3160
PRIMER_RIGHT_10_END_STABILITY=8.2000
3161
PRIMER_PAIR_10_COMPL_ANY=5.00
3162
PRIMER_PAIR_10_COMPL_END=0.00
3163
PRIMER_PRODUCT_SIZE_10=497
3164
PRIMER_PAIR_PENALTY_11=16.9598
3165
PRIMER_LEFT_11_PENALTY=9.051374
3166
PRIMER_RIGHT_11_PENALTY=7.908407
3167
PRIMER_LEFT_11_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
3168
PRIMER_RIGHT_11_SEQUENCE=ACATTTCACTTCTCTTCACACCAC
3169
PRIMER_LEFT_11=93,26
3170
PRIMER_RIGHT_11=589,24
3171
PRIMER_LEFT_11_TM=63.051
3172
PRIMER_RIGHT_11_TM=59.592
3173
PRIMER_LEFT_11_GC_PERCENT=38.462
3174
PRIMER_RIGHT_11_GC_PERCENT=41.667
3175
PRIMER_LEFT_11_SELF_ANY=8.00
3176
PRIMER_RIGHT_11_SELF_ANY=2.00
3177
PRIMER_LEFT_11_SELF_END=2.00
3178
PRIMER_RIGHT_11_SELF_END=0.00
3179
PRIMER_LEFT_11_POSITION_PENALTY=0.000000
3180
PRIMER_RIGHT_11_POSITION_PENALTY=3.500000
3181
PRIMER_LEFT_11_END_STABILITY=6.4000
3182
PRIMER_RIGHT_11_END_STABILITY=7.6000
3183
PRIMER_PAIR_11_COMPL_ANY=5.00
3184
PRIMER_PAIR_11_COMPL_END=0.00
3185
PRIMER_PRODUCT_SIZE_11=497
3186
PRIMER_PAIR_PENALTY_12=17.1262
3187
PRIMER_LEFT_12_PENALTY=9.051374
3188
PRIMER_RIGHT_12_PENALTY=8.074807
3189
PRIMER_LEFT_12_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
3190
PRIMER_RIGHT_12_SEQUENCE=AACATTTCACTTCTCTTCACACCA
3191
PRIMER_LEFT_12=93,26
3192
PRIMER_RIGHT_12=590,24
3193
PRIMER_LEFT_12_TM=63.051
3194
PRIMER_RIGHT_12_TM=60.075
3195
PRIMER_LEFT_12_GC_PERCENT=38.462
3196
PRIMER_RIGHT_12_GC_PERCENT=37.500
3197
PRIMER_LEFT_12_SELF_ANY=8.00
3198
PRIMER_RIGHT_12_SELF_ANY=2.00
3199
PRIMER_LEFT_12_SELF_END=2.00
3200
PRIMER_RIGHT_12_SELF_END=0.00
3201
PRIMER_LEFT_12_POSITION_PENALTY=0.000000
3202
PRIMER_RIGHT_12_POSITION_PENALTY=4.000000
3203
PRIMER_LEFT_12_END_STABILITY=6.4000
3204
PRIMER_RIGHT_12_END_STABILITY=8.2000
3205
PRIMER_PAIR_12_COMPL_ANY=5.00
3206
PRIMER_PAIR_12_COMPL_END=1.00
3207
PRIMER_PRODUCT_SIZE_12=498
3208
PRIMER_PAIR_PENALTY_13=17.5496
3209
PRIMER_LEFT_13_PENALTY=9.051374
3210
PRIMER_RIGHT_13_PENALTY=8.498271
3211
PRIMER_LEFT_13_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
3212
PRIMER_RIGHT_13_SEQUENCE=ATTTCACTTCTCTTCACACCACT
3213
PRIMER_LEFT_13=93,26
3214
PRIMER_RIGHT_13=587,23
3215
PRIMER_LEFT_13_TM=63.051
3216
PRIMER_RIGHT_13_TM=57.002
3217
PRIMER_LEFT_13_GC_PERCENT=38.462
3218
PRIMER_RIGHT_13_GC_PERCENT=39.130
3219
PRIMER_LEFT_13_SELF_ANY=8.00
3220
PRIMER_RIGHT_13_SELF_ANY=2.00
3221
PRIMER_LEFT_13_SELF_END=2.00
3222
PRIMER_RIGHT_13_SELF_END=1.00
3223
PRIMER_LEFT_13_POSITION_PENALTY=0.000000
3224
PRIMER_RIGHT_13_POSITION_PENALTY=2.500000
3225
PRIMER_LEFT_13_END_STABILITY=6.4000
3226
PRIMER_RIGHT_13_END_STABILITY=7.9000
3227
PRIMER_PAIR_13_COMPL_ANY=5.00
3228
PRIMER_PAIR_13_COMPL_END=1.00
3229
PRIMER_PRODUCT_SIZE_13=495
3230
PRIMER_PAIR_PENALTY_14=17.6262
3231
PRIMER_LEFT_14_PENALTY=9.051374
3232
PRIMER_RIGHT_14_PENALTY=8.574807
3233
PRIMER_LEFT_14_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
3234
PRIMER_RIGHT_14_SEQUENCE=CAACATTTCACTTCTCTTCACACC
3235
PRIMER_LEFT_14=93,26
3236
PRIMER_RIGHT_14=591,24
3237
PRIMER_LEFT_14_TM=63.051
3238
PRIMER_RIGHT_14_TM=60.075
3239
PRIMER_LEFT_14_GC_PERCENT=38.462
3240
PRIMER_RIGHT_14_GC_PERCENT=41.667
3241
PRIMER_LEFT_14_SELF_ANY=8.00
3242
PRIMER_RIGHT_14_SELF_ANY=2.00
3243
PRIMER_LEFT_14_SELF_END=2.00
3244
PRIMER_RIGHT_14_SELF_END=0.00
3245
PRIMER_LEFT_14_POSITION_PENALTY=0.000000
3246
PRIMER_RIGHT_14_POSITION_PENALTY=4.500000
3247
PRIMER_LEFT_14_END_STABILITY=6.4000
3248
PRIMER_RIGHT_14_END_STABILITY=7.6000
3249
PRIMER_PAIR_14_COMPL_ANY=5.00
3250
PRIMER_PAIR_14_COMPL_END=1.00
3251
PRIMER_PRODUCT_SIZE_14=499
3252
PRIMER_PAIR_PENALTY_15=17.6905
3253
PRIMER_LEFT_15_PENALTY=9.051374
3254
PRIMER_RIGHT_15_PENALTY=8.639124
3255
PRIMER_LEFT_15_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
3256
PRIMER_RIGHT_15_SEQUENCE=CACTTCTCTTCACACCACTGGTTCT
3257
PRIMER_LEFT_15=93,26
3258
PRIMER_RIGHT_15=583,25
3259
PRIMER_LEFT_15_TM=63.051
3260
PRIMER_RIGHT_15_TM=63.139
3261
PRIMER_LEFT_15_GC_PERCENT=38.462
3262
PRIMER_RIGHT_15_GC_PERCENT=48.000
3263
PRIMER_LEFT_15_SELF_ANY=8.00
3264
PRIMER_RIGHT_15_SELF_ANY=7.00
3265
PRIMER_LEFT_15_SELF_END=2.00
3266
PRIMER_RIGHT_15_SELF_END=3.00
3267
PRIMER_LEFT_15_POSITION_PENALTY=0.000000
3268
PRIMER_RIGHT_15_POSITION_PENALTY=0.500000
3269
PRIMER_LEFT_15_END_STABILITY=6.4000
3270
PRIMER_RIGHT_15_END_STABILITY=6.4000
3271
PRIMER_PAIR_15_COMPL_ANY=5.00
3272
PRIMER_PAIR_15_COMPL_END=1.00
3273
PRIMER_PRODUCT_SIZE_15=491
3274
PRIMER_PAIR_PENALTY_16=18.0352
3275
PRIMER_LEFT_16_PENALTY=9.051374
3276
PRIMER_RIGHT_16_PENALTY=8.983834
3277
PRIMER_LEFT_16_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
3278
PRIMER_RIGHT_16_SEQUENCE=ACATTTCACTTCTCTTCACACCACT
3279
PRIMER_LEFT_16=93,26
3280
PRIMER_RIGHT_16=589,25
3281
PRIMER_LEFT_16_TM=63.051
3282
PRIMER_RIGHT_16_TM=60.484
3283
PRIMER_LEFT_16_GC_PERCENT=38.462
3284
PRIMER_RIGHT_16_GC_PERCENT=40.000
3285
PRIMER_LEFT_16_SELF_ANY=8.00
3286
PRIMER_RIGHT_16_SELF_ANY=2.00
3287
PRIMER_LEFT_16_SELF_END=2.00
3288
PRIMER_RIGHT_16_SELF_END=1.00
3289
PRIMER_LEFT_16_POSITION_PENALTY=0.000000
3290
PRIMER_RIGHT_16_POSITION_PENALTY=3.500000
3291
PRIMER_LEFT_16_END_STABILITY=6.4000
3292
PRIMER_RIGHT_16_END_STABILITY=7.9000
3293
PRIMER_PAIR_16_COMPL_ANY=5.00
3294
PRIMER_PAIR_16_COMPL_END=1.00
3295
PRIMER_PRODUCT_SIZE_16=497
3296
PRIMER_PAIR_PENALTY_17=18.0736
3297
PRIMER_LEFT_17_PENALTY=9.051374
3298
PRIMER_RIGHT_17_PENALTY=9.022192
3299
PRIMER_LEFT_17_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
3300
PRIMER_RIGHT_17_SEQUENCE=ACTTCTCTTCACACCACTGGTTCTCT
3301
PRIMER_LEFT_17=93,26
3302
PRIMER_RIGHT_17=582,26
3303
PRIMER_LEFT_17_TM=63.051
3304
PRIMER_RIGHT_17_TM=63.022
3305
PRIMER_LEFT_17_GC_PERCENT=38.462
3306
PRIMER_RIGHT_17_GC_PERCENT=46.154
3307
PRIMER_LEFT_17_SELF_ANY=8.00
3308
PRIMER_RIGHT_17_SELF_ANY=7.00
3309
PRIMER_LEFT_17_SELF_END=2.00
3310
PRIMER_RIGHT_17_SELF_END=0.00
3311
PRIMER_LEFT_17_POSITION_PENALTY=0.000000
3312
PRIMER_RIGHT_17_POSITION_PENALTY=0.000000
3313
PRIMER_LEFT_17_END_STABILITY=6.4000
3314
PRIMER_RIGHT_17_END_STABILITY=6.4000
3315
PRIMER_PAIR_17_COMPL_ANY=5.00
3316
PRIMER_PAIR_17_COMPL_END=3.00
3317
PRIMER_PRODUCT_SIZE_17=490
3318
PRIMER_PAIR_PENALTY_18=18.6362
3319
PRIMER_LEFT_18_PENALTY=9.051374
3320
PRIMER_RIGHT_18_PENALTY=9.584808
3321
PRIMER_LEFT_18_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
3322
PRIMER_RIGHT_18_SEQUENCE=GCACAACATTTCACTTCTCTTCA
3323
PRIMER_LEFT_18=93,26
3324
PRIMER_RIGHT_18=594,23
3325
PRIMER_LEFT_18_TM=63.051
3326
PRIMER_RIGHT_18_TM=59.415
3327
PRIMER_LEFT_18_GC_PERCENT=38.462
3328
PRIMER_RIGHT_18_GC_PERCENT=39.130
3329
PRIMER_LEFT_18_SELF_ANY=8.00
3330
PRIMER_RIGHT_18_SELF_ANY=2.00
3331
PRIMER_LEFT_18_SELF_END=2.00
3332
PRIMER_RIGHT_18_SELF_END=1.00
3333
PRIMER_LEFT_18_POSITION_PENALTY=0.000000
3334
PRIMER_RIGHT_18_POSITION_PENALTY=6.000000
3335
PRIMER_LEFT_18_END_STABILITY=6.4000
3336
PRIMER_RIGHT_18_END_STABILITY=7.0000
3337
PRIMER_PAIR_18_COMPL_ANY=5.00
3338
PRIMER_PAIR_18_COMPL_END=2.00
3339
PRIMER_PRODUCT_SIZE_18=502
3340
PRIMER_PAIR_PENALTY_19=18.6661
3341
PRIMER_LEFT_19_PENALTY=9.051374
3342
PRIMER_RIGHT_19_PENALTY=9.614678
3343
PRIMER_LEFT_19_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC
3344
PRIMER_RIGHT_19_SEQUENCE=AACATTTCACTTCTCTTCACACC
3345
PRIMER_LEFT_19=93,26
3346
PRIMER_RIGHT_19=590,23
3347
PRIMER_LEFT_19_TM=63.051
3348
PRIMER_RIGHT_19_TM=57.385
3349
PRIMER_LEFT_19_GC_PERCENT=38.462
3350
PRIMER_RIGHT_19_GC_PERCENT=39.130
3351
PRIMER_LEFT_19_SELF_ANY=8.00
3352
PRIMER_RIGHT_19_SELF_ANY=2.00
3353
PRIMER_LEFT_19_SELF_END=2.00
3354
PRIMER_RIGHT_19_SELF_END=0.00
3355
PRIMER_LEFT_19_POSITION_PENALTY=0.000000
3356
PRIMER_RIGHT_19_POSITION_PENALTY=4.000000
3357
PRIMER_LEFT_19_END_STABILITY=6.4000
3358
PRIMER_RIGHT_19_END_STABILITY=7.6000
3359
PRIMER_PAIR_19_COMPL_ANY=5.00
3360
PRIMER_PAIR_19_COMPL_END=1.00
3361
PRIMER_PRODUCT_SIZE_19=498
3362
=
3363
PRIMER_SEQUENCE_ID=X80343/P35
3364
PRIMER_COMMENT=H.sapiens p35 mRNA for regulatory subunit of cdk5 kinase. CDS=98..1021
3365
PRIMER_START_CODON_POSITION=97
3366
SEQUENCE=AAACTCAGAATTTTCGCGGGCTCGGTGAGCGGTTTTATCCCTCCGGCCGGCAGGCTGGGCGCAGGGGGCGAGCCCCCGCCCGGCGCGCAGCAGCACCATGGGCACGGTGCTGTCCCTGTCTCCCAGCTACCGGAAGGCCACGCTGTTTGAGGATGGCGCGGCCACCGTGGGCCACTATACGGCCGTACAGAACAGCAAGAACGCCAAGGACAAGAACCTGAAGCGCCACTCCATCATCTCCGTGCTGCCTTGGAAGAGAATCGTGGCCGTGTCGGCCAAGAAGAAGAACTCCAAGAAGGTGCAGCCTAACAGCAGCTACCAGAACAACATCACGCACCTCAACAATGAGAACCTGAAGAAGTCGCTGTCGTGCGCCAACCTGTCCACATTCGCCCAGCCCCCACCGGCCCAGCCGCCTGCACCCCCGGCCAGCCAGCTCTCGGGTTCCCAGACCGGGGGCTCCTCCTCAGTCAAGAAAGCCCCTCACCCTGCCGTCACCTCCGCAGGGACGCCCAAACGGGTCATCGTCCAGGCGTCCACCAGTGAGCTGCTTCGCTGCCTGGGTGAGTTTCTCTGCCGCCGGTGCTACCGCCTGAAGCACCTGTCCCCCACGGACCCCGTGCTCTGGCTGCGCAGCGTGGACCGCTCGCTGCTTCTGCAGGGCTGGCAGGACCAGGGCTTCATCACGCCGGCCAACGTGGTCTTCCTCTACATGCTCTGCAGGGATGTTATCTCCTCCGAGGTGGGCTCGGATCACGAGCTCCAGGCCGTCCTGCTGACATGCCTGTACCTCTCCTACTCCTACATGGGCAACGAGATCTCCTACCCGCTCAAGCCCTTCCTGGTGGAGAGCTGCAAGGAGGCCTTTTGGGACCGTTGCCTCTCTGTCATCAACCTCATGAGCTCAAAGATGCTGCAGATAAATGCCGACCCACACTACTTCACACAGGTCTTCTCCGACCTGAAGAACGAGAGCGGCCAGGAGGACAAGAAGCGGCTCCTCCTAGGCCTGGATCGGTGAGCACTGTAGCCTGCGTCATGGCTCAAGGATTCAATGCATTTTTAAGAATTTATTATTAAATCAGTTTTGTGTACAG
3367
PRIMER_LEFT_EXPLAIN=considered 8968, would not amplify any of the ORF 6063, GC content failed 212, low tm 125, high tm 1996, high any compl 11, high end compl 4,high 3' stability 33, ok 524
3368
PRIMER_RIGHT_EXPLAIN=considered 8912, GC content failed 682, low tm 430, high tm 6084, high end compl 14,high 3' stability 37, ok 1665
3369
PRIMER_PAIR_EXPLAIN=considered 63, high end compl 6, ok 57
3370
PRIMER_STOP_CODON_POSITION=1018
3371
PRIMER_PAIR_PENALTY=127.4669
3372
PRIMER_LEFT_PENALTY=122.322207
3373
PRIMER_RIGHT_PENALTY=5.144675
3374
PRIMER_LEFT_SEQUENCE=ACGGTGCTGTCCCTGTCTC
3375
PRIMER_RIGHT_SEQUENCE=TACAGTGCTCACCGATCCAG
3376
PRIMER_LEFT=103,19
3377
PRIMER_RIGHT=1028,20
3378
PRIMER_LEFT_TM=61.322
3379
PRIMER_RIGHT_TM=59.855
3380
PRIMER_LEFT_GC_PERCENT=63.158
3381
PRIMER_RIGHT_GC_PERCENT=55.000
3382
PRIMER_LEFT_SELF_ANY=3.00
3383
PRIMER_RIGHT_SELF_ANY=4.00
3384
PRIMER_LEFT_SELF_END=0.00
3385
PRIMER_RIGHT_SELF_END=1.00
3386
PRIMER_LEFT_POSITION_PENALTY=120.000000
3387
PRIMER_RIGHT_POSITION_PENALTY=5.000000
3388
PRIMER_LEFT_END_STABILITY=6.1000
3389
PRIMER_RIGHT_END_STABILITY=8.2000
3390
PRIMER_PAIR_COMPL_ANY=5.00
3391
PRIMER_PAIR_COMPL_END=3.00
3392
PRIMER_PRODUCT_SIZE=926
3393
PRIMER_PAIR_PENALTY_1=127.9669
3394
PRIMER_LEFT_1_PENALTY=122.322207
3395
PRIMER_RIGHT_1_PENALTY=5.644675
3396
PRIMER_LEFT_1_SEQUENCE=ACGGTGCTGTCCCTGTCTC
3397
PRIMER_RIGHT_1_SEQUENCE=CTACAGTGCTCACCGATCCA
3398
PRIMER_LEFT_1=103,19
3399
PRIMER_RIGHT_1=1029,20
3400
PRIMER_LEFT_1_TM=61.322
3401
PRIMER_RIGHT_1_TM=59.855
3402
PRIMER_LEFT_1_GC_PERCENT=63.158
3403
PRIMER_RIGHT_1_GC_PERCENT=55.000
3404
PRIMER_LEFT_1_SELF_ANY=3.00
3405
PRIMER_RIGHT_1_SELF_ANY=4.00
3406
PRIMER_LEFT_1_SELF_END=0.00
3407
PRIMER_RIGHT_1_SELF_END=0.00
3408
PRIMER_LEFT_1_POSITION_PENALTY=120.000000
3409
PRIMER_RIGHT_1_POSITION_PENALTY=5.500000
3410
PRIMER_LEFT_1_END_STABILITY=6.1000
3411
PRIMER_RIGHT_1_END_STABILITY=8.1000
3412
PRIMER_PAIR_1_COMPL_ANY=5.00
3413
PRIMER_PAIR_1_COMPL_END=2.00
3414
PRIMER_PRODUCT_SIZE_1=927
3415
PRIMER_PAIR_PENALTY_2=127.9856
3416
PRIMER_LEFT_2_PENALTY=122.322207
3417
PRIMER_RIGHT_2_PENALTY=5.663428
3418
PRIMER_LEFT_2_SEQUENCE=ACGGTGCTGTCCCTGTCTC
3419
PRIMER_RIGHT_2_SEQUENCE=ACAGTGCTCACCGATCCAG
3420
PRIMER_LEFT_2=103,19
3421
PRIMER_RIGHT_2=1027,19
3422
PRIMER_LEFT_2_TM=61.322
3423
PRIMER_RIGHT_2_TM=59.837
3424
PRIMER_LEFT_2_GC_PERCENT=63.158
3425
PRIMER_RIGHT_2_GC_PERCENT=57.895
3426
PRIMER_LEFT_2_SELF_ANY=3.00
3427
PRIMER_RIGHT_2_SELF_ANY=4.00
3428
PRIMER_LEFT_2_SELF_END=0.00
3429
PRIMER_RIGHT_2_SELF_END=1.00
3430
PRIMER_LEFT_2_POSITION_PENALTY=120.000000
3431
PRIMER_RIGHT_2_POSITION_PENALTY=4.500000
3432
PRIMER_LEFT_2_END_STABILITY=6.1000
3433
PRIMER_RIGHT_2_END_STABILITY=8.2000
3434
PRIMER_PAIR_2_COMPL_ANY=5.00
3435
PRIMER_PAIR_2_COMPL_END=3.00
3436
PRIMER_PRODUCT_SIZE_2=925
3437
PRIMER_PAIR_PENALTY_3=129.1555
3438
PRIMER_LEFT_3_PENALTY=122.322207
3439
PRIMER_RIGHT_3_PENALTY=6.833259
3440
PRIMER_LEFT_3_SEQUENCE=ACGGTGCTGTCCCTGTCTC
3441
PRIMER_RIGHT_3_SEQUENCE=GCTACAGTGCTCACCGATCC
3442
PRIMER_LEFT_3=103,19
3443
PRIMER_RIGHT_3=1030,20
3444
PRIMER_LEFT_3_TM=61.322
3445
PRIMER_RIGHT_3_TM=60.833
3446
PRIMER_LEFT_3_GC_PERCENT=63.158
3447
PRIMER_RIGHT_3_GC_PERCENT=60.000
3448
PRIMER_LEFT_3_SELF_ANY=3.00
3449
PRIMER_RIGHT_3_SELF_ANY=4.00
3450
PRIMER_LEFT_3_SELF_END=0.00
3451
PRIMER_RIGHT_3_SELF_END=2.00
3452
PRIMER_LEFT_3_POSITION_PENALTY=120.000000
3453
PRIMER_RIGHT_3_POSITION_PENALTY=6.000000
3454
PRIMER_LEFT_3_END_STABILITY=6.1000
3455
PRIMER_RIGHT_3_END_STABILITY=7.8000
3456
PRIMER_PAIR_3_COMPL_ANY=5.00
3457
PRIMER_PAIR_3_COMPL_END=3.00
3458
PRIMER_PRODUCT_SIZE_3=928
3459
PRIMER_PAIR_PENALTY_4=129.4246
3460
PRIMER_LEFT_4_PENALTY=122.322207
3461
PRIMER_RIGHT_4_PENALTY=7.102380
3462
PRIMER_LEFT_4_SEQUENCE=ACGGTGCTGTCCCTGTCTC
3463
PRIMER_RIGHT_4_SEQUENCE=AGGCTACAGTGCTCACCGAT
3464
PRIMER_LEFT_4=103,19
3465
PRIMER_RIGHT_4=1032,20
3466
PRIMER_LEFT_4_TM=61.322
3467
PRIMER_RIGHT_4_TM=59.898
3468
PRIMER_LEFT_4_GC_PERCENT=63.158
3469
PRIMER_RIGHT_4_GC_PERCENT=55.000
3470
PRIMER_LEFT_4_SELF_ANY=3.00
3471
PRIMER_RIGHT_4_SELF_ANY=4.00
3472
PRIMER_LEFT_4_SELF_END=0.00
3473
PRIMER_RIGHT_4_SELF_END=2.00
3474
PRIMER_LEFT_4_POSITION_PENALTY=120.000000
3475
PRIMER_RIGHT_4_POSITION_PENALTY=7.000000
3476
PRIMER_LEFT_4_END_STABILITY=6.1000
3477
PRIMER_RIGHT_4_END_STABILITY=9.8000
3478
PRIMER_PAIR_4_COMPL_ANY=5.00
3479
PRIMER_PAIR_4_COMPL_END=3.00
3480
PRIMER_PRODUCT_SIZE_4=930
3481
PRIMER_PAIR_PENALTY_5=129.5803
3482
PRIMER_LEFT_5_PENALTY=122.322207
3483
PRIMER_RIGHT_5_PENALTY=7.258073
3484
PRIMER_LEFT_5_SEQUENCE=ACGGTGCTGTCCCTGTCTC
3485
PRIMER_RIGHT_5_SEQUENCE=TACAGTGCTCACCGATCCA
3486
PRIMER_LEFT_5=103,19
3487
PRIMER_RIGHT_5=1028,19
3488
PRIMER_LEFT_5_TM=61.322
3489
PRIMER_RIGHT_5_TM=58.742
3490
PRIMER_LEFT_5_GC_PERCENT=63.158
3491
PRIMER_RIGHT_5_GC_PERCENT=52.632
3492
PRIMER_LEFT_5_SELF_ANY=3.00
3493
PRIMER_RIGHT_5_SELF_ANY=4.00
3494
PRIMER_LEFT_5_SELF_END=0.00
3495
PRIMER_RIGHT_5_SELF_END=0.00
3496
PRIMER_LEFT_5_POSITION_PENALTY=120.000000
3497
PRIMER_RIGHT_5_POSITION_PENALTY=5.000000
3498
PRIMER_LEFT_5_END_STABILITY=6.1000
3499
PRIMER_RIGHT_5_END_STABILITY=8.1000
3500
PRIMER_PAIR_5_COMPL_ANY=5.00
3501
PRIMER_PAIR_5_COMPL_END=2.00
3502
PRIMER_PRODUCT_SIZE_5=926
3503
PRIMER_PAIR_PENALTY_6=129.6813
3504
PRIMER_LEFT_6_PENALTY=122.322207
3505
PRIMER_RIGHT_6_PENALTY=7.359137
3506
PRIMER_LEFT_6_SEQUENCE=ACGGTGCTGTCCCTGTCTC
3507
PRIMER_RIGHT_6_SEQUENCE=CTACAGTGCTCACCGATCCAG
3508
PRIMER_LEFT_6=103,19
3509
PRIMER_RIGHT_6=1029,21
3510
PRIMER_LEFT_6_TM=61.322
3511
PRIMER_RIGHT_6_TM=60.859
3512
PRIMER_LEFT_6_GC_PERCENT=63.158
3513
PRIMER_RIGHT_6_GC_PERCENT=57.143
3514
PRIMER_LEFT_6_SELF_ANY=3.00
3515
PRIMER_RIGHT_6_SELF_ANY=4.00
3516
PRIMER_LEFT_6_SELF_END=0.00
3517
PRIMER_RIGHT_6_SELF_END=1.00
3518
PRIMER_LEFT_6_POSITION_PENALTY=120.000000
3519
PRIMER_RIGHT_6_POSITION_PENALTY=5.500000
3520
PRIMER_LEFT_6_END_STABILITY=6.1000
3521
PRIMER_RIGHT_6_END_STABILITY=8.2000
3522
PRIMER_PAIR_6_COMPL_ANY=5.00
3523
PRIMER_PAIR_6_COMPL_END=3.00
3524
PRIMER_PRODUCT_SIZE_6=927
3525
PRIMER_PAIR_PENALTY_7=130.1180
3526
PRIMER_LEFT_7_PENALTY=124.973289
3527
PRIMER_RIGHT_7_PENALTY=5.144675
3528
PRIMER_LEFT_7_SEQUENCE=ACGGTGCTGTCCCTGTCTCC
3529
PRIMER_RIGHT_7_SEQUENCE=TACAGTGCTCACCGATCCAG
3530
PRIMER_LEFT_7=103,20
3531
PRIMER_RIGHT_7=1028,20
3532
PRIMER_LEFT_7_TM=64.973
3533
PRIMER_RIGHT_7_TM=59.855
3534
PRIMER_LEFT_7_GC_PERCENT=65.000
3535
PRIMER_RIGHT_7_GC_PERCENT=55.000
3536
PRIMER_LEFT_7_SELF_ANY=3.00
3537
PRIMER_RIGHT_7_SELF_ANY=4.00
3538
PRIMER_LEFT_7_SELF_END=0.00
3539
PRIMER_RIGHT_7_SELF_END=1.00
3540
PRIMER_LEFT_7_POSITION_PENALTY=120.000000
3541
PRIMER_RIGHT_7_POSITION_PENALTY=5.000000
3542
PRIMER_LEFT_7_END_STABILITY=7.9000
3543
PRIMER_RIGHT_7_END_STABILITY=8.2000
3544
PRIMER_PAIR_7_COMPL_ANY=5.00
3545
PRIMER_PAIR_7_COMPL_END=2.00
3546
PRIMER_PRODUCT_SIZE_7=926
3547
PRIMER_PAIR_PENALTY_8=130.6021
3548
PRIMER_LEFT_8_PENALTY=122.322207
3549
PRIMER_RIGHT_8_PENALTY=8.279921
3550
PRIMER_LEFT_8_SEQUENCE=ACGGTGCTGTCCCTGTCTC
3551
PRIMER_RIGHT_8_SEQUENCE=AGTGCTCACCGATCCAGGC
3552
PRIMER_LEFT_8=103,19
3553
PRIMER_RIGHT_8=1025,19
3554
PRIMER_LEFT_8_TM=61.322
3555
PRIMER_RIGHT_8_TM=63.780
3556
PRIMER_LEFT_8_GC_PERCENT=63.158
3557
PRIMER_RIGHT_8_GC_PERCENT=63.158
3558
PRIMER_LEFT_8_SELF_ANY=3.00
3559
PRIMER_RIGHT_8_SELF_ANY=4.00
3560
PRIMER_LEFT_8_SELF_END=0.00
3561
PRIMER_RIGHT_8_SELF_END=2.00
3562
PRIMER_LEFT_8_POSITION_PENALTY=120.000000
3563
PRIMER_RIGHT_8_POSITION_PENALTY=3.500000
3564
PRIMER_LEFT_8_END_STABILITY=6.1000
3565
PRIMER_RIGHT_8_END_STABILITY=9.7000
3566
PRIMER_PAIR_8_COMPL_ANY=5.00
3567
PRIMER_PAIR_8_COMPL_END=3.00
3568
PRIMER_PRODUCT_SIZE_8=923
3569
PRIMER_PAIR_PENALTY_9=130.6180
3570
PRIMER_LEFT_9_PENALTY=124.973289
3571
PRIMER_RIGHT_9_PENALTY=5.644675
3572
PRIMER_LEFT_9_SEQUENCE=ACGGTGCTGTCCCTGTCTCC
3573
PRIMER_RIGHT_9_SEQUENCE=CTACAGTGCTCACCGATCCA
3574
PRIMER_LEFT_9=103,20
3575
PRIMER_RIGHT_9=1029,20
3576
PRIMER_LEFT_9_TM=64.973
3577
PRIMER_RIGHT_9_TM=59.855
3578
PRIMER_LEFT_9_GC_PERCENT=65.000
3579
PRIMER_RIGHT_9_GC_PERCENT=55.000
3580
PRIMER_LEFT_9_SELF_ANY=3.00
3581
PRIMER_RIGHT_9_SELF_ANY=4.00
3582
PRIMER_LEFT_9_SELF_END=0.00
3583
PRIMER_RIGHT_9_SELF_END=0.00
3584
PRIMER_LEFT_9_POSITION_PENALTY=120.000000
3585
PRIMER_RIGHT_9_POSITION_PENALTY=5.500000
3586
PRIMER_LEFT_9_END_STABILITY=7.9000
3587
PRIMER_RIGHT_9_END_STABILITY=8.1000
3588
PRIMER_PAIR_9_COMPL_ANY=5.00
3589
PRIMER_PAIR_9_COMPL_END=1.00
3590
PRIMER_PRODUCT_SIZE_9=927
3591
PRIMER_PAIR_PENALTY_10=130.6367
3592
PRIMER_LEFT_10_PENALTY=124.973289
3593
PRIMER_RIGHT_10_PENALTY=5.663428
3594
PRIMER_LEFT_10_SEQUENCE=ACGGTGCTGTCCCTGTCTCC
3595
PRIMER_RIGHT_10_SEQUENCE=ACAGTGCTCACCGATCCAG
3596
PRIMER_LEFT_10=103,20
3597
PRIMER_RIGHT_10=1027,19
3598
PRIMER_LEFT_10_TM=64.973
3599
PRIMER_RIGHT_10_TM=59.837
3600
PRIMER_LEFT_10_GC_PERCENT=65.000
3601
PRIMER_RIGHT_10_GC_PERCENT=57.895
3602
PRIMER_LEFT_10_SELF_ANY=3.00
3603
PRIMER_RIGHT_10_SELF_ANY=4.00
3604
PRIMER_LEFT_10_SELF_END=0.00
3605
PRIMER_RIGHT_10_SELF_END=1.00
3606
PRIMER_LEFT_10_POSITION_PENALTY=120.000000
3607
PRIMER_RIGHT_10_POSITION_PENALTY=4.500000
3608
PRIMER_LEFT_10_END_STABILITY=7.9000
3609
PRIMER_RIGHT_10_END_STABILITY=8.2000
3610
PRIMER_PAIR_10_COMPL_ANY=5.00
3611
PRIMER_PAIR_10_COMPL_END=2.00
3612
PRIMER_PRODUCT_SIZE_10=925
3613
PRIMER_PAIR_PENALTY_11=130.7353
3614
PRIMER_LEFT_11_PENALTY=122.322207
3615
PRIMER_RIGHT_11_PENALTY=8.413090
3616
PRIMER_LEFT_11_SEQUENCE=ACGGTGCTGTCCCTGTCTC
3617
PRIMER_RIGHT_11_SEQUENCE=AGGCTACAGTGCTCACCGA
3618
PRIMER_LEFT_11=103,19
3619
PRIMER_RIGHT_11=1032,19
3620
PRIMER_LEFT_11_TM=61.322
3621
PRIMER_RIGHT_11_TM=59.587
3622
PRIMER_LEFT_11_GC_PERCENT=63.158
3623
PRIMER_RIGHT_11_GC_PERCENT=57.895
3624
PRIMER_LEFT_11_SELF_ANY=3.00
3625
PRIMER_RIGHT_11_SELF_ANY=4.00
3626
PRIMER_LEFT_11_SELF_END=0.00
3627
PRIMER_RIGHT_11_SELF_END=2.00
3628
PRIMER_LEFT_11_POSITION_PENALTY=120.000000
3629
PRIMER_RIGHT_11_POSITION_PENALTY=7.000000
3630
PRIMER_LEFT_11_END_STABILITY=6.1000
3631
PRIMER_RIGHT_11_END_STABILITY=9.6000
3632
PRIMER_PAIR_11_COMPL_ANY=5.00
3633
PRIMER_PAIR_11_COMPL_END=3.00
3634
PRIMER_PRODUCT_SIZE_11=930
3635
PRIMER_PAIR_PENALTY_12=130.9774
3636
PRIMER_LEFT_12_PENALTY=122.322207
3637
PRIMER_RIGHT_12_PENALTY=8.655174
3638
PRIMER_LEFT_12_SEQUENCE=ACGGTGCTGTCCCTGTCTC
3639
PRIMER_RIGHT_12_SEQUENCE=GGCTACAGTGCTCACCGAT
3640
PRIMER_LEFT_12=103,19
3641
PRIMER_RIGHT_12=1031,19
3642
PRIMER_LEFT_12_TM=61.322
3643
PRIMER_RIGHT_12_TM=58.845
3644
PRIMER_LEFT_12_GC_PERCENT=63.158
3645
PRIMER_RIGHT_12_GC_PERCENT=57.895
3646
PRIMER_LEFT_12_SELF_ANY=3.00
3647
PRIMER_RIGHT_12_SELF_ANY=4.00
3648
PRIMER_LEFT_12_SELF_END=0.00
3649
PRIMER_RIGHT_12_SELF_END=2.00
3650
PRIMER_LEFT_12_POSITION_PENALTY=120.000000
3651
PRIMER_RIGHT_12_POSITION_PENALTY=6.500000
3652
PRIMER_LEFT_12_END_STABILITY=6.1000
3653
PRIMER_RIGHT_12_END_STABILITY=9.8000
3654
PRIMER_PAIR_12_COMPL_ANY=5.00
3655
PRIMER_PAIR_12_COMPL_END=3.00
3656
PRIMER_PRODUCT_SIZE_12=929
3657
PRIMER_PAIR_PENALTY_13=131.7293
3658
PRIMER_LEFT_13_PENALTY=122.322207
3659
PRIMER_RIGHT_13_PENALTY=9.407131
3660
PRIMER_LEFT_13_SEQUENCE=ACGGTGCTGTCCCTGTCTC
3661
PRIMER_RIGHT_13_SEQUENCE=GCAGGCTACAGTGCTCACC
3662
PRIMER_LEFT_13=103,19
3663
PRIMER_RIGHT_13=1034,19
3664
PRIMER_LEFT_13_TM=61.322
3665
PRIMER_RIGHT_13_TM=59.593
3666
PRIMER_LEFT_13_GC_PERCENT=63.158
3667
PRIMER_RIGHT_13_GC_PERCENT=63.158
3668
PRIMER_LEFT_13_SELF_ANY=3.00
3669
PRIMER_RIGHT_13_SELF_ANY=4.00
3670
PRIMER_LEFT_13_SELF_END=0.00
3671
PRIMER_RIGHT_13_SELF_END=2.00
3672
PRIMER_LEFT_13_POSITION_PENALTY=120.000000
3673
PRIMER_RIGHT_13_POSITION_PENALTY=8.000000
3674
PRIMER_LEFT_13_END_STABILITY=6.1000
3675
PRIMER_RIGHT_13_END_STABILITY=7.9000
3676
PRIMER_PAIR_13_COMPL_ANY=5.00
3677
PRIMER_PAIR_13_COMPL_END=3.00
3678
PRIMER_PRODUCT_SIZE_13=932
3679
PRIMER_PAIR_PENALTY_14=131.8065
3680
PRIMER_LEFT_14_PENALTY=124.973289
3681
PRIMER_RIGHT_14_PENALTY=6.833259
3682
PRIMER_LEFT_14_SEQUENCE=ACGGTGCTGTCCCTGTCTCC
3683
PRIMER_RIGHT_14_SEQUENCE=GCTACAGTGCTCACCGATCC
3684
PRIMER_LEFT_14=103,20
3685
PRIMER_RIGHT_14=1030,20
3686
PRIMER_LEFT_14_TM=64.973
3687
PRIMER_RIGHT_14_TM=60.833
3688
PRIMER_LEFT_14_GC_PERCENT=65.000
3689
PRIMER_RIGHT_14_GC_PERCENT=60.000
3690
PRIMER_LEFT_14_SELF_ANY=3.00
3691
PRIMER_RIGHT_14_SELF_ANY=4.00
3692
PRIMER_LEFT_14_SELF_END=0.00
3693
PRIMER_RIGHT_14_SELF_END=2.00
3694
PRIMER_LEFT_14_POSITION_PENALTY=120.000000
3695
PRIMER_RIGHT_14_POSITION_PENALTY=6.000000
3696
PRIMER_LEFT_14_END_STABILITY=7.9000
3697
PRIMER_RIGHT_14_END_STABILITY=7.8000
3698
PRIMER_PAIR_14_COMPL_ANY=5.00
3699
PRIMER_PAIR_14_COMPL_END=1.00
3700
PRIMER_PRODUCT_SIZE_14=928
3701
PRIMER_PAIR_PENALTY_15=132.2314
3702
PRIMER_LEFT_15_PENALTY=124.973289
3703
PRIMER_RIGHT_15_PENALTY=7.258073
3704
PRIMER_LEFT_15_SEQUENCE=ACGGTGCTGTCCCTGTCTCC
3705
PRIMER_RIGHT_15_SEQUENCE=TACAGTGCTCACCGATCCA
3706
PRIMER_LEFT_15=103,20
3707
PRIMER_RIGHT_15=1028,19
3708
PRIMER_LEFT_15_TM=64.973
3709
PRIMER_RIGHT_15_TM=58.742
3710
PRIMER_LEFT_15_GC_PERCENT=65.000
3711
PRIMER_RIGHT_15_GC_PERCENT=52.632
3712
PRIMER_LEFT_15_SELF_ANY=3.00
3713
PRIMER_RIGHT_15_SELF_ANY=4.00
3714
PRIMER_LEFT_15_SELF_END=0.00
3715
PRIMER_RIGHT_15_SELF_END=0.00
3716
PRIMER_LEFT_15_POSITION_PENALTY=120.000000
3717
PRIMER_RIGHT_15_POSITION_PENALTY=5.000000
3718
PRIMER_LEFT_15_END_STABILITY=7.9000
3719
PRIMER_RIGHT_15_END_STABILITY=8.1000
3720
PRIMER_PAIR_15_COMPL_ANY=5.00
3721
PRIMER_PAIR_15_COMPL_END=1.00
3722
PRIMER_PRODUCT_SIZE_15=926
3723
PRIMER_PAIR_PENALTY_16=132.3324
3724
PRIMER_LEFT_16_PENALTY=124.973289
3725
PRIMER_RIGHT_16_PENALTY=7.359137
3726
PRIMER_LEFT_16_SEQUENCE=ACGGTGCTGTCCCTGTCTCC
3727
PRIMER_RIGHT_16_SEQUENCE=CTACAGTGCTCACCGATCCAG
3728
PRIMER_LEFT_16=103,20
3729
PRIMER_RIGHT_16=1029,21
3730
PRIMER_LEFT_16_TM=64.973
3731
PRIMER_RIGHT_16_TM=60.859
3732
PRIMER_LEFT_16_GC_PERCENT=65.000
3733
PRIMER_RIGHT_16_GC_PERCENT=57.143
3734
PRIMER_LEFT_16_SELF_ANY=3.00
3735
PRIMER_RIGHT_16_SELF_ANY=4.00
3736
PRIMER_LEFT_16_SELF_END=0.00
3737
PRIMER_RIGHT_16_SELF_END=1.00
3738
PRIMER_LEFT_16_POSITION_PENALTY=120.000000
3739
PRIMER_RIGHT_16_POSITION_PENALTY=5.500000
3740
PRIMER_LEFT_16_END_STABILITY=7.9000
3741
PRIMER_RIGHT_16_END_STABILITY=8.2000
3742
PRIMER_PAIR_16_COMPL_ANY=5.00
3743
PRIMER_PAIR_16_COMPL_END=2.00
3744
PRIMER_PRODUCT_SIZE_16=927
3745
PRIMER_PAIR_PENALTY_17=132.3926
3746
PRIMER_LEFT_17_PENALTY=122.322207
3747
PRIMER_RIGHT_17_PENALTY=10.070442
3748
PRIMER_LEFT_17_SEQUENCE=ACGGTGCTGTCCCTGTCTC
3749
PRIMER_RIGHT_17_SEQUENCE=CAGGCTACAGTGCTCACCGA
3750
PRIMER_LEFT_17=103,19
3751
PRIMER_RIGHT_17=1033,20
3752
PRIMER_LEFT_17_TM=61.322
3753
PRIMER_RIGHT_17_TM=62.570
3754
PRIMER_LEFT_17_GC_PERCENT=63.158
3755
PRIMER_RIGHT_17_GC_PERCENT=60.000
3756
PRIMER_LEFT_17_SELF_ANY=3.00
3757
PRIMER_RIGHT_17_SELF_ANY=4.00
3758
PRIMER_LEFT_17_SELF_END=0.00
3759
PRIMER_RIGHT_17_SELF_END=2.00
3760
PRIMER_LEFT_17_POSITION_PENALTY=120.000000
3761
PRIMER_RIGHT_17_POSITION_PENALTY=7.500000
3762
PRIMER_LEFT_17_END_STABILITY=6.1000
3763
PRIMER_RIGHT_17_END_STABILITY=9.6000
3764
PRIMER_PAIR_17_COMPL_ANY=5.00
3765
PRIMER_PAIR_17_COMPL_END=3.00
3766
PRIMER_PRODUCT_SIZE_17=931
3767
PRIMER_PAIR_PENALTY_18=132.8596
3768
PRIMER_LEFT_18_PENALTY=124.973289
3769
PRIMER_RIGHT_18_PENALTY=7.886276
3770
PRIMER_LEFT_18_SEQUENCE=ACGGTGCTGTCCCTGTCTCC
3771
PRIMER_RIGHT_18_SEQUENCE=CAGTGCTCACCGATCCAGG
3772
PRIMER_LEFT_18=103,20
3773
PRIMER_RIGHT_18=1026,19
3774
PRIMER_LEFT_18_TM=64.973
3775
PRIMER_RIGHT_18_TM=62.886
3776
PRIMER_LEFT_18_GC_PERCENT=65.000
3777
PRIMER_RIGHT_18_GC_PERCENT=63.158
3778
PRIMER_LEFT_18_SELF_ANY=3.00
3779
PRIMER_RIGHT_18_SELF_ANY=4.00
3780
PRIMER_LEFT_18_SELF_END=0.00
3781
PRIMER_RIGHT_18_SELF_END=3.00
3782
PRIMER_LEFT_18_POSITION_PENALTY=120.000000
3783
PRIMER_RIGHT_18_POSITION_PENALTY=4.000000
3784
PRIMER_LEFT_18_END_STABILITY=7.9000
3785
PRIMER_RIGHT_18_END_STABILITY=9.7000
3786
PRIMER_PAIR_18_COMPL_ANY=5.00
3787
PRIMER_PAIR_18_COMPL_END=3.00
3788
PRIMER_PRODUCT_SIZE_18=924
3789
PRIMER_PAIR_PENALTY_19=132.9859
3790
PRIMER_LEFT_19_PENALTY=122.322207
3791
PRIMER_RIGHT_19_PENALTY=10.663709
3792
PRIMER_LEFT_19_SEQUENCE=ACGGTGCTGTCCCTGTCTC
3793
PRIMER_RIGHT_19_SEQUENCE=GCTACAGTGCTCACCGATCCA
3794
PRIMER_LEFT_19=103,19
3795
PRIMER_RIGHT_19=1030,21
3796
PRIMER_LEFT_19_TM=61.322
3797
PRIMER_RIGHT_19_TM=63.664
3798
PRIMER_LEFT_19_GC_PERCENT=63.158
3799
PRIMER_RIGHT_19_GC_PERCENT=57.143
3800
PRIMER_LEFT_19_SELF_ANY=3.00
3801
PRIMER_RIGHT_19_SELF_ANY=4.00
3802
PRIMER_LEFT_19_SELF_END=0.00
3803
PRIMER_RIGHT_19_SELF_END=0.00
3804
PRIMER_LEFT_19_POSITION_PENALTY=120.000000
3805
PRIMER_RIGHT_19_POSITION_PENALTY=6.000000
3806
PRIMER_LEFT_19_END_STABILITY=6.1000
3807
PRIMER_RIGHT_19_END_STABILITY=8.1000
3808
PRIMER_PAIR_19_COMPL_ANY=5.00
3809
PRIMER_PAIR_19_COMPL_END=3.00
3810
PRIMER_PRODUCT_SIZE_19=928
3811
=
3812
PRIMER_SEQUENCE_ID=L23959/DP1
3813
PRIMER_COMMENT=Homo sapiens E2F-related transcription factor (DP-1) mRNA, complete cds. CDS=38..1270
3814
PRIMER_START_CODON_POSITION=37
3815
SEQUENCE=GGAATTCCGTAGCTATTGATTTCCCGGATCTGGTAACATGGCAAAAGATGCCGGTCTAATTGAAGCCAACGGAGAACTCAAGGTCTTCATAGACCAGAACCTTAGTCCCGGGAAAGGCGTGGTGTCCCTCGTGGCCGTTCACCCCTCCACCGTCAACCCGCTCGGGAAGCAGCTCTTGCCAAAAACCTTTGGACAGTCCAATGTCAACATTGCCCAGCAAGTGGTAATTGGTACGCCTCAGAGACCGGCAGCGTCAAACACCCTGGTGGTAGGAAGCCCACACACCCCCAGCACTCACTTTGCCTCTCAGAACCAGCCTTCCGACTCCTCACCTTGGTCTGCCGGGAAGCGCAACAGGAAAGGAGAGAAGAATGGCAAGGGCCTACGGCATTTCTCCATGAAGGTCTGCGAGAAGGTGCAGAGGAAAGGGACCACTTCCTACAACGAAGTGGCAGACGAGCTGGTTGCGGAGTTCAGTGCTGCCGACAACCACATCTTACCAAACGAGTCAGCTTATGACCAGAAAAACATAAGACGGCGCGTCTACGATGCCTTAAACGTGCTAATGGCCATGAACATCATCTCCAAGGAGAAGAAGGAGATCAAGTGGATTGGTCTGCCCACCAACTCGGCTCAGGAATGTCAGAACTTAGAGGTGGAAAGACAGAGGAGACTTGAAAGAATAAAACAGAAACAGTCTCAACTTCAAGAACTTATTCTACAGCAAATTGCCTTCAAGAACCTGGTGCAGAGAAACCGGCATGCGGAGCAGCAGGCCAGCCGGCCACCGCCACCCAACTCAGTCATCCACCTGCCCTTCATCATCGTCAACACCAGCAAGAAGACGGTCATCGACTGCAGCATCTCCAATGACAAATTTGAGTATCTGTTTAATTTTGACAACACATTTGAAATCCACGATGACATAGAAGTGCTGAAGCGGATGGGCATGGCTTGCGGGCTGGAGTCGGGGAGCTGCTCTGCCGAAGACCTTAAAATGGCCAGAAGTCTGGTCCCCAAGGCTCTGGAGCCATACGTGACAGAAATGGCTCAGGGAACTGTTGGAGGCGTGTTCATCACGACGGCAGGTTCCACGTCTAACGGCACAAGGTTCTCTGCCAGTGACCTGACCAACGGTGCAGATGGGATGCTGGCCACAAGCTCCAATGGGTCTCAGTACAGCGGCTCCAGGGTGGAGACTCCGGTGTCCTACGTCGGGGAGGACGACGAGGAGGACGATGACTTCAACGAGAATGACGAGGACGACTGACGTCCTCCCCACTTCAGATTCGGCTTCAGGAAAACGTTTAGCGAAAAGAAACTTTTTTTTTAATGTGGGTTTTCTGTTTCCTTTTGGCCTAGTCCCAAGAAGATATTGGTAAGCTATTGAATTTAGATATGCACCTCTGATAAGCAAGGATTGTTTCCCGTAGATTAGGA
3816
PRIMER_LEFT_EXPLAIN=considered 11963, would not amplify any of the ORF 8309, GC content failed 29, low tm 476, high tm 2059, high end compl 3,high 3' stability 47, ok 1040
3817
PRIMER_RIGHT_EXPLAIN=considered 11769, GC content failed 99, low tm 1773, high tm 6322, long poly-x seq 25,high 3' stability 49, ok 3501
3818
PRIMER_PAIR_EXPLAIN=considered 52, ok 52
3819
PRIMER_STOP_CODON_POSITION=1267
3820
PRIMER_PAIR_PENALTY=4.4475
3821
PRIMER_LEFT_PENALTY=4.039125
3822
PRIMER_RIGHT_PENALTY=0.408401
3823
PRIMER_LEFT_SEQUENCE=ATGGCAAAAGATGCCGGTCT
3824
PRIMER_RIGHT_SEQUENCE=AGTCGTCCTCGTCATTCTCG
3825
PRIMER_LEFT=37,20
3826
PRIMER_RIGHT=1267,20
3827
PRIMER_LEFT_TM=64.039
3828
PRIMER_RIGHT_TM=60.408
3829
PRIMER_LEFT_GC_PERCENT=50.000
3830
PRIMER_RIGHT_GC_PERCENT=55.000
3831
PRIMER_LEFT_SELF_ANY=4.00
3832
PRIMER_RIGHT_SELF_ANY=2.00
3833
PRIMER_LEFT_SELF_END=2.00
3834
PRIMER_RIGHT_SELF_END=2.00
3835
PRIMER_LEFT_POSITION_PENALTY=0.000000
3836
PRIMER_RIGHT_POSITION_PENALTY=0.000000
3837
PRIMER_LEFT_END_STABILITY=7.6000
3838
PRIMER_RIGHT_END_STABILITY=8.4000
3839
PRIMER_PAIR_COMPL_ANY=4.00
3840
PRIMER_PAIR_COMPL_END=0.00
3841
PRIMER_PRODUCT_SIZE=1231
3842
PRIMER_PAIR_PENALTY_1=4.6661
3843
PRIMER_LEFT_1_PENALTY=4.257663
3844
PRIMER_RIGHT_1_PENALTY=0.408401
3845
PRIMER_LEFT_1_SEQUENCE=ATGGCAAAAGATGCCGGTC
3846
PRIMER_RIGHT_1_SEQUENCE=AGTCGTCCTCGTCATTCTCG
3847
PRIMER_LEFT_1=37,19
3848
PRIMER_RIGHT_1=1267,20
3849
PRIMER_LEFT_1_TM=63.258
3850
PRIMER_RIGHT_1_TM=60.408
3851
PRIMER_LEFT_1_GC_PERCENT=52.632
3852
PRIMER_RIGHT_1_GC_PERCENT=55.000
3853
PRIMER_LEFT_1_SELF_ANY=4.00
3854
PRIMER_RIGHT_1_SELF_ANY=2.00
3855
PRIMER_LEFT_1_SELF_END=2.00
3856
PRIMER_RIGHT_1_SELF_END=2.00
3857
PRIMER_LEFT_1_POSITION_PENALTY=0.000000
3858
PRIMER_RIGHT_1_POSITION_PENALTY=0.000000
3859
PRIMER_LEFT_1_END_STABILITY=9.6000
3860
PRIMER_RIGHT_1_END_STABILITY=8.4000
3861
PRIMER_PAIR_1_COMPL_ANY=4.00
3862
PRIMER_PAIR_1_COMPL_END=1.00
3863
PRIMER_PRODUCT_SIZE_1=1231
3864
PRIMER_PAIR_PENALTY_2=5.3122
3865
PRIMER_LEFT_2_PENALTY=4.903833
3866
PRIMER_RIGHT_2_PENALTY=0.408401
3867
PRIMER_LEFT_2_SEQUENCE=ATGGCAAAAGATGCCGGTCTA
3868
PRIMER_RIGHT_2_SEQUENCE=AGTCGTCCTCGTCATTCTCG
3869
PRIMER_LEFT_2=37,21
3870
PRIMER_RIGHT_2=1267,20
3871
PRIMER_LEFT_2_TM=63.904
3872
PRIMER_RIGHT_2_TM=60.408
3873
PRIMER_LEFT_2_GC_PERCENT=47.619
3874
PRIMER_RIGHT_2_GC_PERCENT=55.000
3875
PRIMER_LEFT_2_SELF_ANY=4.00
3876
PRIMER_RIGHT_2_SELF_ANY=2.00
3877
PRIMER_LEFT_2_SELF_END=2.00
3878
PRIMER_RIGHT_2_SELF_END=2.00
3879
PRIMER_LEFT_2_POSITION_PENALTY=0.000000
3880
PRIMER_RIGHT_2_POSITION_PENALTY=0.000000
3881
PRIMER_LEFT_2_END_STABILITY=5.4000
3882
PRIMER_RIGHT_2_END_STABILITY=8.4000
3883
PRIMER_PAIR_2_COMPL_ANY=4.00
3884
PRIMER_PAIR_2_COMPL_END=1.00
3885
PRIMER_PRODUCT_SIZE_2=1231
3886
PRIMER_PAIR_PENALTY_3=6.1605
3887
PRIMER_LEFT_3_PENALTY=4.039125
3888
PRIMER_RIGHT_3_PENALTY=2.121401
3889
PRIMER_LEFT_3_SEQUENCE=ATGGCAAAAGATGCCGGTCT
3890
PRIMER_RIGHT_3_SEQUENCE=CAGTCGTCCTCGTCATTCTC
3891
PRIMER_LEFT_3=37,20
3892
PRIMER_RIGHT_3=1268,20
3893
PRIMER_LEFT_3_TM=64.039
3894
PRIMER_RIGHT_3_TM=58.379
3895
PRIMER_LEFT_3_GC_PERCENT=50.000
3896
PRIMER_RIGHT_3_GC_PERCENT=55.000
3897
PRIMER_LEFT_3_SELF_ANY=4.00
3898
PRIMER_RIGHT_3_SELF_ANY=2.00
3899
PRIMER_LEFT_3_SELF_END=2.00
3900
PRIMER_RIGHT_3_SELF_END=0.00
3901
PRIMER_LEFT_3_POSITION_PENALTY=0.000000
3902
PRIMER_RIGHT_3_POSITION_PENALTY=0.500000
3903
PRIMER_LEFT_3_END_STABILITY=7.6000
3904
PRIMER_RIGHT_3_END_STABILITY=6.7000
3905
PRIMER_PAIR_3_COMPL_ANY=4.00
3906
PRIMER_PAIR_3_COMPL_END=0.00
3907
PRIMER_PRODUCT_SIZE_3=1232
3908
PRIMER_PAIR_PENALTY_4=6.2743
3909
PRIMER_LEFT_4_PENALTY=4.039125
3910
PRIMER_RIGHT_4_PENALTY=2.235177
3911
PRIMER_LEFT_4_SEQUENCE=ATGGCAAAAGATGCCGGTCT
3912
PRIMER_RIGHT_4_SEQUENCE=AGTCGTCCTCGTCATTCTCGT
3913
PRIMER_LEFT_4=37,20
3914
PRIMER_RIGHT_4=1267,21
3915
PRIMER_LEFT_4_TM=64.039
3916
PRIMER_RIGHT_4_TM=61.235
3917
PRIMER_LEFT_4_GC_PERCENT=50.000
3918
PRIMER_RIGHT_4_GC_PERCENT=52.381
3919
PRIMER_LEFT_4_SELF_ANY=4.00
3920
PRIMER_RIGHT_4_SELF_ANY=2.00
3921
PRIMER_LEFT_4_SELF_END=2.00
3922
PRIMER_RIGHT_4_SELF_END=0.00
3923
PRIMER_LEFT_4_POSITION_PENALTY=0.000000
3924
PRIMER_RIGHT_4_POSITION_PENALTY=0.000000
3925
PRIMER_LEFT_4_END_STABILITY=7.6000
3926
PRIMER_RIGHT_4_END_STABILITY=8.1000
3927
PRIMER_PAIR_4_COMPL_ANY=4.00
3928
PRIMER_PAIR_4_COMPL_END=0.00
3929
PRIMER_PRODUCT_SIZE_4=1231
3930
PRIMER_PAIR_PENALTY_5=6.3791
3931
PRIMER_LEFT_5_PENALTY=4.257663
3932
PRIMER_RIGHT_5_PENALTY=2.121401
3933
PRIMER_LEFT_5_SEQUENCE=ATGGCAAAAGATGCCGGTC
3934
PRIMER_RIGHT_5_SEQUENCE=CAGTCGTCCTCGTCATTCTC
3935
PRIMER_LEFT_5=37,19
3936
PRIMER_RIGHT_5=1268,20
3937
PRIMER_LEFT_5_TM=63.258
3938
PRIMER_RIGHT_5_TM=58.379
3939
PRIMER_LEFT_5_GC_PERCENT=52.632
3940
PRIMER_RIGHT_5_GC_PERCENT=55.000
3941
PRIMER_LEFT_5_SELF_ANY=4.00
3942
PRIMER_RIGHT_5_SELF_ANY=2.00
3943
PRIMER_LEFT_5_SELF_END=2.00
3944
PRIMER_RIGHT_5_SELF_END=0.00
3945
PRIMER_LEFT_5_POSITION_PENALTY=0.000000
3946
PRIMER_RIGHT_5_POSITION_PENALTY=0.500000
3947
PRIMER_LEFT_5_END_STABILITY=9.6000
3948
PRIMER_RIGHT_5_END_STABILITY=6.7000
3949
PRIMER_PAIR_5_COMPL_ANY=4.00
3950
PRIMER_PAIR_5_COMPL_END=1.00
3951
PRIMER_PRODUCT_SIZE_5=1232
3952
PRIMER_PAIR_PENALTY_6=6.4408
3953
PRIMER_LEFT_6_PENALTY=4.039125
3954
PRIMER_RIGHT_6_PENALTY=2.401642
3955
PRIMER_LEFT_6_SEQUENCE=ATGGCAAAAGATGCCGGTCT
3956
PRIMER_RIGHT_6_SEQUENCE=TCAGTCGTCCTCGTCATTCTC
3957
PRIMER_LEFT_6=37,20
3958
PRIMER_RIGHT_6=1269,21
3959
PRIMER_LEFT_6_TM=64.039
3960
PRIMER_RIGHT_6_TM=60.402
3961
PRIMER_LEFT_6_GC_PERCENT=50.000
3962
PRIMER_RIGHT_6_GC_PERCENT=52.381
3963
PRIMER_LEFT_6_SELF_ANY=4.00
3964
PRIMER_RIGHT_6_SELF_ANY=2.00
3965
PRIMER_LEFT_6_SELF_END=2.00
3966
PRIMER_RIGHT_6_SELF_END=0.00
3967
PRIMER_LEFT_6_POSITION_PENALTY=0.000000
3968
PRIMER_RIGHT_6_POSITION_PENALTY=1.000000
3969
PRIMER_LEFT_6_END_STABILITY=7.6000
3970
PRIMER_RIGHT_6_END_STABILITY=6.7000
3971
PRIMER_PAIR_6_COMPL_ANY=4.00
3972
PRIMER_PAIR_6_COMPL_END=0.00
3973
PRIMER_PRODUCT_SIZE_6=1233
3974
PRIMER_PAIR_PENALTY_7=6.4928
3975
PRIMER_LEFT_7_PENALTY=4.257663
3976
PRIMER_RIGHT_7_PENALTY=2.235177
3977
PRIMER_LEFT_7_SEQUENCE=ATGGCAAAAGATGCCGGTC
3978
PRIMER_RIGHT_7_SEQUENCE=AGTCGTCCTCGTCATTCTCGT
3979
PRIMER_LEFT_7=37,19
3980
PRIMER_RIGHT_7=1267,21
3981
PRIMER_LEFT_7_TM=63.258
3982
PRIMER_RIGHT_7_TM=61.235
3983
PRIMER_LEFT_7_GC_PERCENT=52.632
3984
PRIMER_RIGHT_7_GC_PERCENT=52.381
3985
PRIMER_LEFT_7_SELF_ANY=4.00
3986
PRIMER_RIGHT_7_SELF_ANY=2.00
3987
PRIMER_LEFT_7_SELF_END=2.00
3988
PRIMER_RIGHT_7_SELF_END=0.00
3989
PRIMER_LEFT_7_POSITION_PENALTY=0.000000
3990
PRIMER_RIGHT_7_POSITION_PENALTY=0.000000
3991
PRIMER_LEFT_7_END_STABILITY=9.6000
3992
PRIMER_RIGHT_7_END_STABILITY=8.1000
3993
PRIMER_PAIR_7_COMPL_ANY=4.00
3994
PRIMER_PAIR_7_COMPL_END=0.00
3995
PRIMER_PRODUCT_SIZE_7=1231
3996
PRIMER_PAIR_PENALTY_8=6.6593
3997
PRIMER_LEFT_8_PENALTY=4.257663
3998
PRIMER_RIGHT_8_PENALTY=2.401642
3999
PRIMER_LEFT_8_SEQUENCE=ATGGCAAAAGATGCCGGTC
4000
PRIMER_RIGHT_8_SEQUENCE=TCAGTCGTCCTCGTCATTCTC
4001
PRIMER_LEFT_8=37,19
4002
PRIMER_RIGHT_8=1269,21
4003
PRIMER_LEFT_8_TM=63.258
4004
PRIMER_RIGHT_8_TM=60.402
4005
PRIMER_LEFT_8_GC_PERCENT=52.632
4006
PRIMER_RIGHT_8_GC_PERCENT=52.381
4007
PRIMER_LEFT_8_SELF_ANY=4.00
4008
PRIMER_RIGHT_8_SELF_ANY=2.00
4009
PRIMER_LEFT_8_SELF_END=2.00
4010
PRIMER_RIGHT_8_SELF_END=0.00
4011
PRIMER_LEFT_8_POSITION_PENALTY=0.000000
4012
PRIMER_RIGHT_8_POSITION_PENALTY=1.000000
4013
PRIMER_LEFT_8_END_STABILITY=9.6000
4014
PRIMER_RIGHT_8_END_STABILITY=6.7000
4015
PRIMER_PAIR_8_COMPL_ANY=4.00
4016
PRIMER_PAIR_8_COMPL_END=1.00
4017
PRIMER_PRODUCT_SIZE_8=1233
4018
PRIMER_PAIR_PENALTY_9=6.6605
4019
PRIMER_LEFT_9_PENALTY=4.039125
4020
PRIMER_RIGHT_9_PENALTY=2.621401
4021
PRIMER_LEFT_9_SEQUENCE=ATGGCAAAAGATGCCGGTCT
4022
PRIMER_RIGHT_9_SEQUENCE=TCAGTCGTCCTCGTCATTCT
4023
PRIMER_LEFT_9=37,20
4024
PRIMER_RIGHT_9=1269,20
4025
PRIMER_LEFT_9_TM=64.039
4026
PRIMER_RIGHT_9_TM=58.379
4027
PRIMER_LEFT_9_GC_PERCENT=50.000
4028
PRIMER_RIGHT_9_GC_PERCENT=50.000
4029
PRIMER_LEFT_9_SELF_ANY=4.00
4030
PRIMER_RIGHT_9_SELF_ANY=2.00
4031
PRIMER_LEFT_9_SELF_END=2.00
4032
PRIMER_RIGHT_9_SELF_END=0.00
4033
PRIMER_LEFT_9_POSITION_PENALTY=0.000000
4034
PRIMER_RIGHT_9_POSITION_PENALTY=1.000000
4035
PRIMER_LEFT_9_END_STABILITY=7.6000
4036
PRIMER_RIGHT_9_END_STABILITY=6.6000
4037
PRIMER_PAIR_9_COMPL_ANY=4.00
4038
PRIMER_PAIR_9_COMPL_END=0.00
4039
PRIMER_PRODUCT_SIZE_9=1233
4040
PRIMER_PAIR_PENALTY_10=6.8791
4041
PRIMER_LEFT_10_PENALTY=4.257663
4042
PRIMER_RIGHT_10_PENALTY=2.621401
4043
PRIMER_LEFT_10_SEQUENCE=ATGGCAAAAGATGCCGGTC
4044
PRIMER_RIGHT_10_SEQUENCE=TCAGTCGTCCTCGTCATTCT
4045
PRIMER_LEFT_10=37,19
4046
PRIMER_RIGHT_10=1269,20
4047
PRIMER_LEFT_10_TM=63.258
4048
PRIMER_RIGHT_10_TM=58.379
4049
PRIMER_LEFT_10_GC_PERCENT=52.632
4050
PRIMER_RIGHT_10_GC_PERCENT=50.000
4051
PRIMER_LEFT_10_SELF_ANY=4.00
4052
PRIMER_RIGHT_10_SELF_ANY=2.00
4053
PRIMER_LEFT_10_SELF_END=2.00
4054
PRIMER_RIGHT_10_SELF_END=0.00
4055
PRIMER_LEFT_10_POSITION_PENALTY=0.000000
4056
PRIMER_RIGHT_10_POSITION_PENALTY=1.000000
4057
PRIMER_LEFT_10_END_STABILITY=9.6000
4058
PRIMER_RIGHT_10_END_STABILITY=6.6000
4059
PRIMER_PAIR_10_COMPL_ANY=4.00
4060
PRIMER_PAIR_10_COMPL_END=1.00
4061
PRIMER_PRODUCT_SIZE_10=1233
4062
PRIMER_PAIR_PENALTY_11=7.0252
4063
PRIMER_LEFT_11_PENALTY=4.903833
4064
PRIMER_RIGHT_11_PENALTY=2.121401
4065
PRIMER_LEFT_11_SEQUENCE=ATGGCAAAAGATGCCGGTCTA
4066
PRIMER_RIGHT_11_SEQUENCE=CAGTCGTCCTCGTCATTCTC
4067
PRIMER_LEFT_11=37,21
4068
PRIMER_RIGHT_11=1268,20
4069
PRIMER_LEFT_11_TM=63.904
4070
PRIMER_RIGHT_11_TM=58.379
4071
PRIMER_LEFT_11_GC_PERCENT=47.619
4072
PRIMER_RIGHT_11_GC_PERCENT=55.000
4073
PRIMER_LEFT_11_SELF_ANY=4.00
4074
PRIMER_RIGHT_11_SELF_ANY=2.00
4075
PRIMER_LEFT_11_SELF_END=2.00
4076
PRIMER_RIGHT_11_SELF_END=0.00
4077
PRIMER_LEFT_11_POSITION_PENALTY=0.000000
4078
PRIMER_RIGHT_11_POSITION_PENALTY=0.500000
4079
PRIMER_LEFT_11_END_STABILITY=5.4000
4080
PRIMER_RIGHT_11_END_STABILITY=6.7000
4081
PRIMER_PAIR_11_COMPL_ANY=4.00
4082
PRIMER_PAIR_11_COMPL_END=1.00
4083
PRIMER_PRODUCT_SIZE_11=1232
4084
PRIMER_PAIR_PENALTY_12=7.1390
4085
PRIMER_LEFT_12_PENALTY=4.903833
4086
PRIMER_RIGHT_12_PENALTY=2.235177
4087
PRIMER_LEFT_12_SEQUENCE=ATGGCAAAAGATGCCGGTCTA
4088
PRIMER_RIGHT_12_SEQUENCE=AGTCGTCCTCGTCATTCTCGT
4089
PRIMER_LEFT_12=37,21
4090
PRIMER_RIGHT_12=1267,21
4091
PRIMER_LEFT_12_TM=63.904
4092
PRIMER_RIGHT_12_TM=61.235
4093
PRIMER_LEFT_12_GC_PERCENT=47.619
4094
PRIMER_RIGHT_12_GC_PERCENT=52.381
4095
PRIMER_LEFT_12_SELF_ANY=4.00
4096
PRIMER_RIGHT_12_SELF_ANY=2.00
4097
PRIMER_LEFT_12_SELF_END=2.00
4098
PRIMER_RIGHT_12_SELF_END=0.00
4099
PRIMER_LEFT_12_POSITION_PENALTY=0.000000
4100
PRIMER_RIGHT_12_POSITION_PENALTY=0.000000
4101
PRIMER_LEFT_12_END_STABILITY=5.4000
4102
PRIMER_RIGHT_12_END_STABILITY=8.1000
4103
PRIMER_PAIR_12_COMPL_ANY=4.00
4104
PRIMER_PAIR_12_COMPL_END=1.00
4105
PRIMER_PRODUCT_SIZE_12=1231
4106
PRIMER_PAIR_PENALTY_13=7.2026
4107
PRIMER_LEFT_13_PENALTY=4.039125
4108
PRIMER_RIGHT_13_PENALTY=3.163428
4109
PRIMER_LEFT_13_SEQUENCE=ATGGCAAAAGATGCCGGTCT
4110
PRIMER_RIGHT_13_SEQUENCE=CGTCAGTCGTCCTCGTCAT
4111
PRIMER_LEFT_13=37,20
4112
PRIMER_RIGHT_13=1271,19
4113
PRIMER_LEFT_13_TM=64.039
4114
PRIMER_RIGHT_13_TM=59.837
4115
PRIMER_LEFT_13_GC_PERCENT=50.000
4116
PRIMER_RIGHT_13_GC_PERCENT=57.895
4117
PRIMER_LEFT_13_SELF_ANY=4.00
4118
PRIMER_RIGHT_13_SELF_ANY=3.00
4119
PRIMER_LEFT_13_SELF_END=2.00
4120
PRIMER_RIGHT_13_SELF_END=2.00
4121
PRIMER_LEFT_13_POSITION_PENALTY=0.000000
4122
PRIMER_RIGHT_13_POSITION_PENALTY=2.000000
4123
PRIMER_LEFT_13_END_STABILITY=7.6000
4124
PRIMER_RIGHT_13_END_STABILITY=6.3000
4125
PRIMER_PAIR_13_COMPL_ANY=4.00
4126
PRIMER_PAIR_13_COMPL_END=2.00
4127
PRIMER_PRODUCT_SIZE_13=1235
4128
PRIMER_PAIR_PENALTY_14=7.2196
4129
PRIMER_LEFT_14_PENALTY=4.039125
4130
PRIMER_RIGHT_14_PENALTY=3.180452
4131
PRIMER_LEFT_14_SEQUENCE=ATGGCAAAAGATGCCGGTCT
4132
PRIMER_RIGHT_14_SEQUENCE=GTCAGTCGTCCTCGTCATTCT
4133
PRIMER_LEFT_14=37,20
4134
PRIMER_RIGHT_14=1270,21
4135
PRIMER_LEFT_14_TM=64.039
4136
PRIMER_RIGHT_14_TM=59.320
4137
PRIMER_LEFT_14_GC_PERCENT=50.000
4138
PRIMER_RIGHT_14_GC_PERCENT=52.381
4139
PRIMER_LEFT_14_SELF_ANY=4.00
4140
PRIMER_RIGHT_14_SELF_ANY=2.00
4141
PRIMER_LEFT_14_SELF_END=2.00
4142
PRIMER_RIGHT_14_SELF_END=0.00
4143
PRIMER_LEFT_14_POSITION_PENALTY=0.000000
4144
PRIMER_RIGHT_14_POSITION_PENALTY=1.500000
4145
PRIMER_LEFT_14_END_STABILITY=7.6000
4146
PRIMER_RIGHT_14_END_STABILITY=6.6000
4147
PRIMER_PAIR_14_COMPL_ANY=4.00
4148
PRIMER_PAIR_14_COMPL_END=0.00
4149
PRIMER_PRODUCT_SIZE_14=1234
4150
PRIMER_PAIR_PENALTY_15=7.2848
4151
PRIMER_LEFT_15_PENALTY=4.039125
4152
PRIMER_RIGHT_15_PENALTY=3.245695
4153
PRIMER_LEFT_15_SEQUENCE=ATGGCAAAAGATGCCGGTCT
4154
PRIMER_RIGHT_15_SEQUENCE=ACGTCAGTCGTCCTCGTCAT
4155
PRIMER_LEFT_15=37,20
4156
PRIMER_RIGHT_15=1272,20
4157
PRIMER_LEFT_15_TM=64.039
4158
PRIMER_RIGHT_15_TM=60.746
4159
PRIMER_LEFT_15_GC_PERCENT=50.000
4160
PRIMER_RIGHT_15_GC_PERCENT=55.000
4161
PRIMER_LEFT_15_SELF_ANY=4.00
4162
PRIMER_RIGHT_15_SELF_ANY=5.00
4163
PRIMER_LEFT_15_SELF_END=2.00
4164
PRIMER_RIGHT_15_SELF_END=2.00
4165
PRIMER_LEFT_15_POSITION_PENALTY=0.000000
4166
PRIMER_RIGHT_15_POSITION_PENALTY=2.500000
4167
PRIMER_LEFT_15_END_STABILITY=7.6000
4168
PRIMER_RIGHT_15_END_STABILITY=6.3000
4169
PRIMER_PAIR_15_COMPL_ANY=4.00
4170
PRIMER_PAIR_15_COMPL_END=1.00
4171
PRIMER_PRODUCT_SIZE_15=1236
4172
PRIMER_PAIR_PENALTY_16=7.3055
4173
PRIMER_LEFT_16_PENALTY=4.903833
4174
PRIMER_RIGHT_16_PENALTY=2.401642
4175
PRIMER_LEFT_16_SEQUENCE=ATGGCAAAAGATGCCGGTCTA
4176
PRIMER_RIGHT_16_SEQUENCE=TCAGTCGTCCTCGTCATTCTC
4177
PRIMER_LEFT_16=37,21
4178
PRIMER_RIGHT_16=1269,21
4179
PRIMER_LEFT_16_TM=63.904
4180
PRIMER_RIGHT_16_TM=60.402
4181
PRIMER_LEFT_16_GC_PERCENT=47.619
4182
PRIMER_RIGHT_16_GC_PERCENT=52.381
4183
PRIMER_LEFT_16_SELF_ANY=4.00
4184
PRIMER_RIGHT_16_SELF_ANY=2.00
4185
PRIMER_LEFT_16_SELF_END=2.00
4186
PRIMER_RIGHT_16_SELF_END=0.00
4187
PRIMER_LEFT_16_POSITION_PENALTY=0.000000
4188
PRIMER_RIGHT_16_POSITION_PENALTY=1.000000
4189
PRIMER_LEFT_16_END_STABILITY=5.4000
4190
PRIMER_RIGHT_16_END_STABILITY=6.7000
4191
PRIMER_PAIR_16_COMPL_ANY=4.00
4192
PRIMER_PAIR_16_COMPL_END=1.00
4193
PRIMER_PRODUCT_SIZE_16=1233
4194
PRIMER_PAIR_PENALTY_17=7.3104
4195
PRIMER_LEFT_17_PENALTY=6.902047
4196
PRIMER_RIGHT_17_PENALTY=0.408401
4197
PRIMER_LEFT_17_SEQUENCE=ATGGCAAAAGATGCCGGTCTAA
4198
PRIMER_RIGHT_17_SEQUENCE=AGTCGTCCTCGTCATTCTCG
4199
PRIMER_LEFT_17=37,22
4200
PRIMER_RIGHT_17=1267,20
4201
PRIMER_LEFT_17_TM=64.902
4202
PRIMER_RIGHT_17_TM=60.408
4203
PRIMER_LEFT_17_GC_PERCENT=45.455
4204
PRIMER_RIGHT_17_GC_PERCENT=55.000
4205
PRIMER_LEFT_17_SELF_ANY=4.00
4206
PRIMER_RIGHT_17_SELF_ANY=2.00
4207
PRIMER_LEFT_17_SELF_END=1.00
4208
PRIMER_RIGHT_17_SELF_END=2.00
4209
PRIMER_LEFT_17_POSITION_PENALTY=0.000000
4210
PRIMER_RIGHT_17_POSITION_PENALTY=0.000000
4211
PRIMER_LEFT_17_END_STABILITY=6.0000
4212
PRIMER_RIGHT_17_END_STABILITY=8.4000
4213
PRIMER_PAIR_17_COMPL_ANY=4.00
4214
PRIMER_PAIR_17_COMPL_END=0.00
4215
PRIMER_PRODUCT_SIZE_17=1231
4216
PRIMER_PAIR_PENALTY_18=7.3184
4217
PRIMER_LEFT_18_PENALTY=4.039125
4218
PRIMER_RIGHT_18_PENALTY=3.279241
4219
PRIMER_LEFT_18_SEQUENCE=ATGGCAAAAGATGCCGGTCT
4220
PRIMER_RIGHT_18_SEQUENCE=CGTCAGTCGTCCTCGTCATT
4221
PRIMER_LEFT_18=37,20
4222
PRIMER_RIGHT_18=1271,20
4223
PRIMER_LEFT_18_TM=64.039
4224
PRIMER_RIGHT_18_TM=61.279
4225
PRIMER_LEFT_18_GC_PERCENT=50.000
4226
PRIMER_RIGHT_18_GC_PERCENT=55.000
4227
PRIMER_LEFT_18_SELF_ANY=4.00
4228
PRIMER_RIGHT_18_SELF_ANY=3.00
4229
PRIMER_LEFT_18_SELF_END=2.00
4230
PRIMER_RIGHT_18_SELF_END=1.00
4231
PRIMER_LEFT_18_POSITION_PENALTY=0.000000
4232
PRIMER_RIGHT_18_POSITION_PENALTY=2.000000
4233
PRIMER_LEFT_18_END_STABILITY=7.6000
4234
PRIMER_RIGHT_18_END_STABILITY=6.9000
4235
PRIMER_PAIR_18_COMPL_ANY=4.00
4236
PRIMER_PAIR_18_COMPL_END=1.00
4237
PRIMER_PRODUCT_SIZE_18=1235
4238
PRIMER_PAIR_PENALTY_19=7.3228
4239
PRIMER_LEFT_19_PENALTY=4.039125
4240
PRIMER_RIGHT_19_PENALTY=3.283719
4241
PRIMER_LEFT_19_SEQUENCE=ATGGCAAAAGATGCCGGTCT
4242
PRIMER_RIGHT_19_SEQUENCE=GTCAGTCGTCCTCGTCATTC
4243
PRIMER_LEFT_19=37,20
4244
PRIMER_RIGHT_19=1270,20
4245
PRIMER_LEFT_19_TM=64.039
4246
PRIMER_RIGHT_19_TM=58.216
4247
PRIMER_LEFT_19_GC_PERCENT=50.000
4248
PRIMER_RIGHT_19_GC_PERCENT=55.000
4249
PRIMER_LEFT_19_SELF_ANY=4.00
4250
PRIMER_RIGHT_19_SELF_ANY=2.00
4251
PRIMER_LEFT_19_SELF_END=2.00
4252
PRIMER_RIGHT_19_SELF_END=0.00
4253
PRIMER_LEFT_19_POSITION_PENALTY=0.000000
4254
PRIMER_RIGHT_19_POSITION_PENALTY=1.500000
4255
PRIMER_LEFT_19_END_STABILITY=7.6000
4256
PRIMER_RIGHT_19_END_STABILITY=6.9000
4257
PRIMER_PAIR_19_COMPL_ANY=4.00
4258
PRIMER_PAIR_19_COMPL_END=0.00
4259
PRIMER_PRODUCT_SIZE_19=1234
4260
=
4261
PRIMER_SEQUENCE_ID=X98032/E6AP
4262
PRIMER_COMMENT=H.sapiens mRNA for E6-AP isoform-I. CDS=1..2559
4263
PRIMER_START_CODON_POSITION=0
4264
SEQUENCE=ATGAAGCGAGCAGCTGCAAAGCATCTAATAGAACGCTACTACCACCAGTTAACTGAGGGCTGTGGAAATGAAGCCTGCACGAATGAGTTTTGTGCTTCCTGTCCAACTTTTCTTCGTATGGATAATAATGCAGCAGCTATTAAAGCCCTCGAGCTTTATAAGATTAATGCAAAACTCTGTGATCCTCATCCCTCCAAGAAAGGAGCAAGCTCAGCTTACCTTGAGAACTCGAAAGGTGCCCCCAACAACTCCTGCTCTGAGATAAAAATGAACAAGAAAGGCGCTAGAATTGATTTTAAAGATGTGACTTACTTAACAGAAGAGAAGGTATATGAAATTCTTGAATTATGTAGAGAAAGAGAGGATTATTCCCCTTTAATCCGTGTTATTGGAAGAGTTTTTTCTAGTGCTGAGGCATTGGTACAGAGCTTCCGGAAAGTTAAACAACACACCAAGGAAGAACTGAAATCTCTTCAAGCAAAAGATGAAGACAAAGATGAAGATGAAAAGGAAAAAGCTGCATGTTCTGCTGCTGCTATGGAAGAAGACTCAGAAGCATCTTCCTCAAGGATAGGTGATAGCTCACAGGGAGACAACAATTTGCAAAAATTAGGCCCTGATGATGTGTCTGTGGATATTGATGCCATTAGAAGGGTCTACACCAGATTGCTCTCTAATGAAAAAATTGAAACTGCCTTTCTCAATGCACTTGTATATTTGTCACCTAACGTGGAATGTGACTTGACGTATCACAATGTATACTCTCGAGATCCTAATTATCTGAATTTGTTCATTATCGGAATGGAGAATAGAAATCTCCACAGTCCTGAATATCTGGAAATGGCTTTGCCATTATTTTGCAAAGCGATGAGCAAGCTACCCCTTGCAGCCCAAGGAAAACTGATCAGACTGTGGTCTAAATACAATGCAGACCAGATTCGGAGAATGATGGAGACATTTCAGCAACTTATTACTTATAAAGTCATAAGCAATGAATTTAACAGTCGAAATCTAGTGAATGATGATGATGCCATTGTTGCTGCTTCGAAGTGCTTGAAAATGGTTTACTATGCAAATGTAGTGGGAGGGGAAGTGGACACAAATCACAATGAAGAAGATGATGAAGAGCCCATCCCTGAGTCCAGCGAGCTGACACTTCAGGAACTTTTGGGAGAAGAAAGAAGAAACAAGAAAGGTCCTCGAGTGGACCCCCTGGAAACTGAACTTGGTGTTAAAACCCTGGATTGTCGAAAACCACTTATCCCTTTTGAAGAGTTTATTAATGAACCACTGAATGAGGTTCTAGAAATGGATAAAGATTATACTTTTTTCAAAGTAGAAACAGAGAACAAATTCTCTTTTATGACATGTCCCTTTATATTGAATGCTGTCACAAAGAATTTGGGATTATATTATGACAATAGAATTCGCATGTACAGTGAACGAAGAATCACTGTTCTCTACAGCTTAGTTCAAGGACAGCAGTTGAATCCATATTTGAGACTCAAAGTTAGACGTGACCATATCATAGATGATGCACTTGTCCGGCTAGAGATGATCGCTATGGAAAATCCTGCAGACTTGAAGAAGCAGTTGTATGTGGAATTTGAAGGAGAACAAGGAGTTGATGAGGGAGGTGTTTCCAAAGAATTTTTTCAGCTGGTTGTGGAGGAAATCTTCAATCCAGATATTGGTATGTTCACATACGATGAATCTACAAAATTGTTTTGGTTTAATCCATCTTCTTTTGAAACTGAGGGTCAGTTTACTCTGATTGGCATAGTACTGGGTCTGGCTATTTACAATAACTGTATACTGGATGTACATTTTCCCATGGTTGTCTACAGGAAGCTAATGGGGAAAAAAGGAACTTTTCGTGACTTGGGAGACTCTCACCCAGTTCTATATCAGAGTTTAAAAGATTTATTGGAGTATGAAGGGAATGTGGAAGATGACATGATGATCACTTTCCAGATATCACAGACAGATCTTTTTGGTAACCCAATGATGTATGATCTAAAGGAAAATGGTGATAAAATTCCAATTACAAATGAAAACAGGAAGGAATTTGTCAATCTTTATTCTGACTACATTCTCAATAAATCAGTAGAAAAACAGTTCAAGGCTTTTCGGAGAGGTTTTCATATGGTGACCAATGAATCTCCCTTAAAGTACTTATTCAGACCAGAAGAAATTGAATTGCTTATATGTGGAAGCCGGAATCTAGATTTCCAAGCACTAGAAGAAACTACAGAATATGACGGTGGCTATACCAGGGACTCTGTTCTGATTAGGGAGTTCTGGGAAATCGTTCATTCATTTACAGATGAACAGAAAAGACTCTTCTTGCAGTTTACAACGGGCACAGACAGAGCACCTGTGGGAGGACTAGGAAAATTAAAGATGATTATAGCCAAAAATGGCCCAGACACAGAAAGGTTACCTACATCTCATACTTGCTTTAATGTGCTTTTACTTCCGGAATACTCAAGCAAAGAAAAACTTAAAGAGAGATTGTTGAAGGCCATCACGTATGCCAAAGGATTTGGCATGCTGTAA
4265
PRIMER_LEFT_EXPLAIN=considered 21507, would not amplify any of the ORF 14282, GC content failed 55, low tm 3071, high tm 1071, high any compl 19, high end compl 17, long poly-x seq 102,high 3' stability 49, ok 2841
4266
PRIMER_RIGHT_EXPLAIN=considered 21345, GC content failed 229, low tm 9108, high tm 3197, high end compl 7, long poly-x seq 106,high 3' stability 53, ok 8645
4267
PRIMER_PAIR_EXPLAIN=considered 48, high end compl 6, ok 42
4268
PRIMER_STOP_CODON_POSITION=2556
4269
PRIMER_PAIR_PENALTY=6.7537
4270
PRIMER_LEFT_PENALTY=4.494664
4271
PRIMER_RIGHT_PENALTY=2.259083
4272
PRIMER_LEFT_SEQUENCE=ATGAAGCGAGCAGCTGCAAA
4273
PRIMER_RIGHT_SEQUENCE=TACAGCATGCCAAATCCTTT
4274
PRIMER_LEFT=0,20
4275
PRIMER_RIGHT=2557,20
4276
PRIMER_LEFT_TM=64.495
4277
PRIMER_RIGHT_TM=58.241
4278
PRIMER_LEFT_GC_PERCENT=50.000
4279
PRIMER_RIGHT_GC_PERCENT=40.000
4280
PRIMER_LEFT_SELF_ANY=8.00
4281
PRIMER_RIGHT_SELF_ANY=6.00
4282
PRIMER_LEFT_SELF_END=2.00
4283
PRIMER_RIGHT_SELF_END=3.00
4284
PRIMER_LEFT_POSITION_PENALTY=0.000000
4285
PRIMER_RIGHT_POSITION_PENALTY=0.500000
4286
PRIMER_LEFT_END_STABILITY=8.8000
4287
PRIMER_RIGHT_END_STABILITY=8.5000
4288
PRIMER_PAIR_COMPL_ANY=5.00
4289
PRIMER_PAIR_COMPL_END=3.00
4290
PRIMER_PRODUCT_SIZE=2558
4291
PRIMER_PAIR_PENALTY_1=6.8946
4292
PRIMER_LEFT_1_PENALTY=4.494664
4293
PRIMER_RIGHT_1_PENALTY=2.399914
4294
PRIMER_LEFT_1_SEQUENCE=ATGAAGCGAGCAGCTGCAAA
4295
PRIMER_RIGHT_1_SEQUENCE=TTACAGCATGCCAAATCCTTT
4296
PRIMER_LEFT_1=0,20
4297
PRIMER_RIGHT_1=2558,21
4298
PRIMER_LEFT_1_TM=64.495
4299
PRIMER_RIGHT_1_TM=59.600
4300
PRIMER_LEFT_1_GC_PERCENT=50.000
4301
PRIMER_RIGHT_1_GC_PERCENT=38.095
4302
PRIMER_LEFT_1_SELF_ANY=8.00
4303
PRIMER_RIGHT_1_SELF_ANY=6.00
4304
PRIMER_LEFT_1_SELF_END=2.00
4305
PRIMER_RIGHT_1_SELF_END=3.00
4306
PRIMER_LEFT_1_POSITION_PENALTY=0.000000
4307
PRIMER_RIGHT_1_POSITION_PENALTY=1.000000
4308
PRIMER_LEFT_1_END_STABILITY=8.8000
4309
PRIMER_RIGHT_1_END_STABILITY=8.5000
4310
PRIMER_PAIR_1_COMPL_ANY=6.00
4311
PRIMER_PAIR_1_COMPL_END=3.00
4312
PRIMER_PRODUCT_SIZE_1=2559
4313
PRIMER_PAIR_PENALTY_2=7.2537
4314
PRIMER_LEFT_2_PENALTY=4.494664
4315
PRIMER_RIGHT_2_PENALTY=2.759083
4316
PRIMER_LEFT_2_SEQUENCE=ATGAAGCGAGCAGCTGCAAA
4317
PRIMER_RIGHT_2_SEQUENCE=TTACAGCATGCCAAATCCTT
4318
PRIMER_LEFT_2=0,20
4319
PRIMER_RIGHT_2=2558,20
4320
PRIMER_LEFT_2_TM=64.495
4321
PRIMER_RIGHT_2_TM=58.241
4322
PRIMER_LEFT_2_GC_PERCENT=50.000
4323
PRIMER_RIGHT_2_GC_PERCENT=40.000
4324
PRIMER_LEFT_2_SELF_ANY=8.00
4325
PRIMER_RIGHT_2_SELF_ANY=6.00
4326
PRIMER_LEFT_2_SELF_END=2.00
4327
PRIMER_RIGHT_2_SELF_END=1.00
4328
PRIMER_LEFT_2_POSITION_PENALTY=0.000000
4329
PRIMER_RIGHT_2_POSITION_PENALTY=1.000000
4330
PRIMER_LEFT_2_END_STABILITY=8.8000
4331
PRIMER_RIGHT_2_END_STABILITY=8.2000
4332
PRIMER_PAIR_2_COMPL_ANY=6.00
4333
PRIMER_PAIR_2_COMPL_END=3.00
4334
PRIMER_PRODUCT_SIZE_2=2559
4335
PRIMER_PAIR_PENALTY_3=7.3356
4336
PRIMER_LEFT_3_PENALTY=4.494664
4337
PRIMER_RIGHT_3_PENALTY=2.840968
4338
PRIMER_LEFT_3_SEQUENCE=ATGAAGCGAGCAGCTGCAAA
4339
PRIMER_RIGHT_3_SEQUENCE=ACAGCATGCCAAATCCTTT
4340
PRIMER_LEFT_3=0,20
4341
PRIMER_RIGHT_3=2556,19
4342
PRIMER_LEFT_3_TM=64.495
4343
PRIMER_RIGHT_3_TM=58.159
4344
PRIMER_LEFT_3_GC_PERCENT=50.000
4345
PRIMER_RIGHT_3_GC_PERCENT=42.105
4346
PRIMER_LEFT_3_SELF_ANY=8.00
4347
PRIMER_RIGHT_3_SELF_ANY=6.00
4348
PRIMER_LEFT_3_SELF_END=2.00
4349
PRIMER_RIGHT_3_SELF_END=3.00
4350
PRIMER_LEFT_3_POSITION_PENALTY=0.000000
4351
PRIMER_RIGHT_3_POSITION_PENALTY=0.000000
4352
PRIMER_LEFT_3_END_STABILITY=8.8000
4353
PRIMER_RIGHT_3_END_STABILITY=8.5000
4354
PRIMER_PAIR_3_COMPL_ANY=5.00
4355
PRIMER_PAIR_3_COMPL_END=3.00
4356
PRIMER_PRODUCT_SIZE_3=2557
4357
PRIMER_PAIR_PENALTY_4=127.1022
4358
PRIMER_LEFT_4_PENALTY=124.343098
4359
PRIMER_RIGHT_4_PENALTY=2.759083
4360
PRIMER_LEFT_4_SEQUENCE=CGAGCAGCTGCAAAGCATC
4361
PRIMER_RIGHT_4_SEQUENCE=TTACAGCATGCCAAATCCTT
4362
PRIMER_LEFT_4=6,19
4363
PRIMER_RIGHT_4=2558,20
4364
PRIMER_LEFT_4_TM=63.343
4365
PRIMER_RIGHT_4_TM=58.241
4366
PRIMER_LEFT_4_GC_PERCENT=57.895
4367
PRIMER_RIGHT_4_GC_PERCENT=40.000
4368
PRIMER_LEFT_4_SELF_ANY=8.00
4369
PRIMER_RIGHT_4_SELF_ANY=6.00
4370
PRIMER_LEFT_4_SELF_END=3.00
4371
PRIMER_RIGHT_4_SELF_END=1.00
4372
PRIMER_LEFT_4_POSITION_PENALTY=120.000000
4373
PRIMER_RIGHT_4_POSITION_PENALTY=1.000000
4374
PRIMER_LEFT_4_END_STABILITY=8.1000
4375
PRIMER_RIGHT_4_END_STABILITY=8.2000
4376
PRIMER_PAIR_4_COMPL_ANY=6.00
4377
PRIMER_PAIR_4_COMPL_END=3.00
4378
PRIMER_PRODUCT_SIZE_4=2553
4379
PRIMER_PAIR_PENALTY_5=127.7645
4380
PRIMER_LEFT_5_PENALTY=125.005410
4381
PRIMER_RIGHT_5_PENALTY=2.759083
4382
PRIMER_LEFT_5_SEQUENCE=CGAGCAGCTGCAAAGCATCTA
4383
PRIMER_RIGHT_5_SEQUENCE=TTACAGCATGCCAAATCCTT
4384
PRIMER_LEFT_5=6,21
4385
PRIMER_RIGHT_5=2558,20
4386
PRIMER_LEFT_5_TM=64.005
4387
PRIMER_RIGHT_5_TM=58.241
4388
PRIMER_LEFT_5_GC_PERCENT=52.381
4389
PRIMER_RIGHT_5_GC_PERCENT=40.000
4390
PRIMER_LEFT_5_SELF_ANY=8.00
4391
PRIMER_RIGHT_5_SELF_ANY=6.00
4392
PRIMER_LEFT_5_SELF_END=3.00
4393
PRIMER_RIGHT_5_SELF_END=1.00
4394
PRIMER_LEFT_5_POSITION_PENALTY=120.000000
4395
PRIMER_RIGHT_5_POSITION_PENALTY=1.000000
4396
PRIMER_LEFT_5_END_STABILITY=5.6000
4397
PRIMER_RIGHT_5_END_STABILITY=8.2000
4398
PRIMER_PAIR_5_COMPL_ANY=6.00
4399
PRIMER_PAIR_5_COMPL_END=3.00
4400
PRIMER_PRODUCT_SIZE_5=2553
4401
PRIMER_PAIR_PENALTY_6=131.1391
4402
PRIMER_LEFT_6_PENALTY=128.880021
4403
PRIMER_RIGHT_6_PENALTY=2.259083
4404
PRIMER_LEFT_6_SEQUENCE=CGAGCAGCTGCAAAGCATCTAATA
4405
PRIMER_RIGHT_6_SEQUENCE=TACAGCATGCCAAATCCTTT
4406
PRIMER_LEFT_6=6,24
4407
PRIMER_RIGHT_6=2557,20
4408
PRIMER_LEFT_6_TM=64.880
4409
PRIMER_RIGHT_6_TM=58.241
4410
PRIMER_LEFT_6_GC_PERCENT=45.833
4411
PRIMER_RIGHT_6_GC_PERCENT=40.000
4412
PRIMER_LEFT_6_SELF_ANY=8.00
4413
PRIMER_RIGHT_6_SELF_ANY=6.00
4414
PRIMER_LEFT_6_SELF_END=3.00
4415
PRIMER_RIGHT_6_SELF_END=3.00
4416
PRIMER_LEFT_6_POSITION_PENALTY=120.000000
4417
PRIMER_RIGHT_6_POSITION_PENALTY=0.500000
4418
PRIMER_LEFT_6_END_STABILITY=5.2000
4419
PRIMER_RIGHT_6_END_STABILITY=8.5000
4420
PRIMER_PAIR_6_COMPL_ANY=5.00
4421
PRIMER_PAIR_6_COMPL_END=3.00
4422
PRIMER_PRODUCT_SIZE_6=2552
4423
PRIMER_PAIR_PENALTY_7=131.2799
4424
PRIMER_LEFT_7_PENALTY=128.880021
4425
PRIMER_RIGHT_7_PENALTY=2.399914
4426
PRIMER_LEFT_7_SEQUENCE=CGAGCAGCTGCAAAGCATCTAATA
4427
PRIMER_RIGHT_7_SEQUENCE=TTACAGCATGCCAAATCCTTT
4428
PRIMER_LEFT_7=6,24
4429
PRIMER_RIGHT_7=2558,21
4430
PRIMER_LEFT_7_TM=64.880
4431
PRIMER_RIGHT_7_TM=59.600
4432
PRIMER_LEFT_7_GC_PERCENT=45.833
4433
PRIMER_RIGHT_7_GC_PERCENT=38.095
4434
PRIMER_LEFT_7_SELF_ANY=8.00
4435
PRIMER_RIGHT_7_SELF_ANY=6.00
4436
PRIMER_LEFT_7_SELF_END=3.00
4437
PRIMER_RIGHT_7_SELF_END=3.00
4438
PRIMER_LEFT_7_POSITION_PENALTY=120.000000
4439
PRIMER_RIGHT_7_POSITION_PENALTY=1.000000
4440
PRIMER_LEFT_7_END_STABILITY=5.2000
4441
PRIMER_RIGHT_7_END_STABILITY=8.5000
4442
PRIMER_PAIR_7_COMPL_ANY=6.00
4443
PRIMER_PAIR_7_COMPL_END=3.00
4444
PRIMER_PRODUCT_SIZE_7=2553
4445
PRIMER_PAIR_PENALTY_8=131.6391
4446
PRIMER_LEFT_8_PENALTY=128.880021
4447
PRIMER_RIGHT_8_PENALTY=2.759083
4448
PRIMER_LEFT_8_SEQUENCE=CGAGCAGCTGCAAAGCATCTAATA
4449
PRIMER_RIGHT_8_SEQUENCE=TTACAGCATGCCAAATCCTT
4450
PRIMER_LEFT_8=6,24
4451
PRIMER_RIGHT_8=2558,20
4452
PRIMER_LEFT_8_TM=64.880
4453
PRIMER_RIGHT_8_TM=58.241
4454
PRIMER_LEFT_8_GC_PERCENT=45.833
4455
PRIMER_RIGHT_8_GC_PERCENT=40.000
4456
PRIMER_LEFT_8_SELF_ANY=8.00
4457
PRIMER_RIGHT_8_SELF_ANY=6.00
4458
PRIMER_LEFT_8_SELF_END=3.00
4459
PRIMER_RIGHT_8_SELF_END=1.00
4460
PRIMER_LEFT_8_POSITION_PENALTY=120.000000
4461
PRIMER_RIGHT_8_POSITION_PENALTY=1.000000
4462
PRIMER_LEFT_8_END_STABILITY=5.2000
4463
PRIMER_RIGHT_8_END_STABILITY=8.2000
4464
PRIMER_PAIR_8_COMPL_ANY=6.00
4465
PRIMER_PAIR_8_COMPL_END=2.00
4466
PRIMER_PRODUCT_SIZE_8=2553
4467
PRIMER_PAIR_PENALTY_9=131.7210
4468
PRIMER_LEFT_9_PENALTY=128.880021
4469
PRIMER_RIGHT_9_PENALTY=2.840968
4470
PRIMER_LEFT_9_SEQUENCE=CGAGCAGCTGCAAAGCATCTAATA
4471
PRIMER_RIGHT_9_SEQUENCE=ACAGCATGCCAAATCCTTT
4472
PRIMER_LEFT_9=6,24
4473
PRIMER_RIGHT_9=2556,19
4474
PRIMER_LEFT_9_TM=64.880
4475
PRIMER_RIGHT_9_TM=58.159
4476
PRIMER_LEFT_9_GC_PERCENT=45.833
4477
PRIMER_RIGHT_9_GC_PERCENT=42.105
4478
PRIMER_LEFT_9_SELF_ANY=8.00
4479
PRIMER_RIGHT_9_SELF_ANY=6.00
4480
PRIMER_LEFT_9_SELF_END=3.00
4481
PRIMER_RIGHT_9_SELF_END=3.00
4482
PRIMER_LEFT_9_POSITION_PENALTY=120.000000
4483
PRIMER_RIGHT_9_POSITION_PENALTY=0.000000
4484
PRIMER_LEFT_9_END_STABILITY=5.2000
4485
PRIMER_RIGHT_9_END_STABILITY=8.5000
4486
PRIMER_PAIR_9_COMPL_ANY=5.00
4487
PRIMER_PAIR_9_COMPL_END=3.00
4488
PRIMER_PRODUCT_SIZE_9=2551
4489
PRIMER_PAIR_PENALTY_10=183.9646
4490
PRIMER_LEFT_10_PENALTY=181.705510
4491
PRIMER_RIGHT_10_PENALTY=2.259083
4492
PRIMER_LEFT_10_SEQUENCE=GCAGCTGCAAAGCATCTAAT
4493
PRIMER_RIGHT_10_SEQUENCE=TACAGCATGCCAAATCCTTT
4494
PRIMER_LEFT_10=9,20
4495
PRIMER_RIGHT_10=2557,20
4496
PRIMER_LEFT_10_TM=58.294
4497
PRIMER_RIGHT_10_TM=58.241
4498
PRIMER_LEFT_10_GC_PERCENT=45.000
4499
PRIMER_RIGHT_10_GC_PERCENT=40.000
4500
PRIMER_LEFT_10_SELF_ANY=8.00
4501
PRIMER_RIGHT_10_SELF_ANY=6.00
4502
PRIMER_LEFT_10_SELF_END=2.00
4503
PRIMER_RIGHT_10_SELF_END=3.00
4504
PRIMER_LEFT_10_POSITION_PENALTY=180.000000
4505
PRIMER_RIGHT_10_POSITION_PENALTY=0.500000
4506
PRIMER_LEFT_10_END_STABILITY=5.9000
4507
PRIMER_RIGHT_10_END_STABILITY=8.5000
4508
PRIMER_PAIR_10_COMPL_ANY=5.00
4509
PRIMER_PAIR_10_COMPL_END=2.00
4510
PRIMER_PRODUCT_SIZE_10=2549
4511
PRIMER_PAIR_PENALTY_11=184.1054
4512
PRIMER_LEFT_11_PENALTY=181.705510
4513
PRIMER_RIGHT_11_PENALTY=2.399914
4514
PRIMER_LEFT_11_SEQUENCE=GCAGCTGCAAAGCATCTAAT
4515
PRIMER_RIGHT_11_SEQUENCE=TTACAGCATGCCAAATCCTTT
4516
PRIMER_LEFT_11=9,20
4517
PRIMER_RIGHT_11=2558,21
4518
PRIMER_LEFT_11_TM=58.294
4519
PRIMER_RIGHT_11_TM=59.600
4520
PRIMER_LEFT_11_GC_PERCENT=45.000
4521
PRIMER_RIGHT_11_GC_PERCENT=38.095
4522
PRIMER_LEFT_11_SELF_ANY=8.00
4523
PRIMER_RIGHT_11_SELF_ANY=6.00
4524
PRIMER_LEFT_11_SELF_END=2.00
4525
PRIMER_RIGHT_11_SELF_END=3.00
4526
PRIMER_LEFT_11_POSITION_PENALTY=180.000000
4527
PRIMER_RIGHT_11_POSITION_PENALTY=1.000000
4528
PRIMER_LEFT_11_END_STABILITY=5.9000
4529
PRIMER_RIGHT_11_END_STABILITY=8.5000
4530
PRIMER_PAIR_11_COMPL_ANY=6.00
4531
PRIMER_PAIR_11_COMPL_END=2.00
4532
PRIMER_PRODUCT_SIZE_11=2550
4533
PRIMER_PAIR_PENALTY_12=184.4646
4534
PRIMER_LEFT_12_PENALTY=181.705510
4535
PRIMER_RIGHT_12_PENALTY=2.759083
4536
PRIMER_LEFT_12_SEQUENCE=GCAGCTGCAAAGCATCTAAT
4537
PRIMER_RIGHT_12_SEQUENCE=TTACAGCATGCCAAATCCTT
4538
PRIMER_LEFT_12=9,20
4539
PRIMER_RIGHT_12=2558,20
4540
PRIMER_LEFT_12_TM=58.294
4541
PRIMER_RIGHT_12_TM=58.241
4542
PRIMER_LEFT_12_GC_PERCENT=45.000
4543
PRIMER_RIGHT_12_GC_PERCENT=40.000
4544
PRIMER_LEFT_12_SELF_ANY=8.00
4545
PRIMER_RIGHT_12_SELF_ANY=6.00
4546
PRIMER_LEFT_12_SELF_END=2.00
4547
PRIMER_RIGHT_12_SELF_END=1.00
4548
PRIMER_LEFT_12_POSITION_PENALTY=180.000000
4549
PRIMER_RIGHT_12_POSITION_PENALTY=1.000000
4550
PRIMER_LEFT_12_END_STABILITY=5.9000
4551
PRIMER_RIGHT_12_END_STABILITY=8.2000
4552
PRIMER_PAIR_12_COMPL_ANY=6.00
4553
PRIMER_PAIR_12_COMPL_END=1.00
4554
PRIMER_PRODUCT_SIZE_12=2550
4555
PRIMER_PAIR_PENALTY_13=184.5465
4556
PRIMER_LEFT_13_PENALTY=181.705510
4557
PRIMER_RIGHT_13_PENALTY=2.840968
4558
PRIMER_LEFT_13_SEQUENCE=GCAGCTGCAAAGCATCTAAT
4559
PRIMER_RIGHT_13_SEQUENCE=ACAGCATGCCAAATCCTTT
4560
PRIMER_LEFT_13=9,20
4561
PRIMER_RIGHT_13=2556,19
4562
PRIMER_LEFT_13_TM=58.294
4563
PRIMER_RIGHT_13_TM=58.159
4564
PRIMER_LEFT_13_GC_PERCENT=45.000
4565
PRIMER_RIGHT_13_GC_PERCENT=42.105
4566
PRIMER_LEFT_13_SELF_ANY=8.00
4567
PRIMER_RIGHT_13_SELF_ANY=6.00
4568
PRIMER_LEFT_13_SELF_END=2.00
4569
PRIMER_RIGHT_13_SELF_END=3.00
4570
PRIMER_LEFT_13_POSITION_PENALTY=180.000000
4571
PRIMER_RIGHT_13_POSITION_PENALTY=0.000000
4572
PRIMER_LEFT_13_END_STABILITY=5.9000
4573
PRIMER_RIGHT_13_END_STABILITY=8.5000
4574
PRIMER_PAIR_13_COMPL_ANY=5.00
4575
PRIMER_PAIR_13_COMPL_END=2.00
4576
PRIMER_PRODUCT_SIZE_13=2548
4577
PRIMER_PAIR_PENALTY_14=184.8909
4578
PRIMER_LEFT_14_PENALTY=182.631776
4579
PRIMER_RIGHT_14_PENALTY=2.259083
4580
PRIMER_LEFT_14_SEQUENCE=GCAGCTGCAAAGCATCTAATA
4581
PRIMER_RIGHT_14_SEQUENCE=TACAGCATGCCAAATCCTTT
4582
PRIMER_LEFT_14=9,21
4583
PRIMER_RIGHT_14=2557,20
4584
PRIMER_LEFT_14_TM=58.368
4585
PRIMER_RIGHT_14_TM=58.241
4586
PRIMER_LEFT_14_GC_PERCENT=42.857
4587
PRIMER_RIGHT_14_GC_PERCENT=40.000
4588
PRIMER_LEFT_14_SELF_ANY=8.00
4589
PRIMER_RIGHT_14_SELF_ANY=6.00
4590
PRIMER_LEFT_14_SELF_END=3.00
4591
PRIMER_RIGHT_14_SELF_END=3.00
4592
PRIMER_LEFT_14_POSITION_PENALTY=180.000000
4593
PRIMER_RIGHT_14_POSITION_PENALTY=0.500000
4594
PRIMER_LEFT_14_END_STABILITY=5.2000
4595
PRIMER_RIGHT_14_END_STABILITY=8.5000
4596
PRIMER_PAIR_14_COMPL_ANY=5.00
4597
PRIMER_PAIR_14_COMPL_END=3.00
4598
PRIMER_PRODUCT_SIZE_14=2549
4599
PRIMER_PAIR_PENALTY_15=185.0317
4600
PRIMER_LEFT_15_PENALTY=182.631776
4601
PRIMER_RIGHT_15_PENALTY=2.399914
4602
PRIMER_LEFT_15_SEQUENCE=GCAGCTGCAAAGCATCTAATA
4603
PRIMER_RIGHT_15_SEQUENCE=TTACAGCATGCCAAATCCTTT
4604
PRIMER_LEFT_15=9,21
4605
PRIMER_RIGHT_15=2558,21
4606
PRIMER_LEFT_15_TM=58.368
4607
PRIMER_RIGHT_15_TM=59.600
4608
PRIMER_LEFT_15_GC_PERCENT=42.857
4609
PRIMER_RIGHT_15_GC_PERCENT=38.095
4610
PRIMER_LEFT_15_SELF_ANY=8.00
4611
PRIMER_RIGHT_15_SELF_ANY=6.00
4612
PRIMER_LEFT_15_SELF_END=3.00
4613
PRIMER_RIGHT_15_SELF_END=3.00
4614
PRIMER_LEFT_15_POSITION_PENALTY=180.000000
4615
PRIMER_RIGHT_15_POSITION_PENALTY=1.000000
4616
PRIMER_LEFT_15_END_STABILITY=5.2000
4617
PRIMER_RIGHT_15_END_STABILITY=8.5000
4618
PRIMER_PAIR_15_COMPL_ANY=6.00
4619
PRIMER_PAIR_15_COMPL_END=3.00
4620
PRIMER_PRODUCT_SIZE_15=2550
4621
PRIMER_PAIR_PENALTY_16=185.3558
4622
PRIMER_LEFT_16_PENALTY=183.096742
4623
PRIMER_RIGHT_16_PENALTY=2.259083
4624
PRIMER_LEFT_16_SEQUENCE=GCAGCTGCAAAGCATCTAA
4625
PRIMER_RIGHT_16_SEQUENCE=TACAGCATGCCAAATCCTTT
4626
PRIMER_LEFT_16=9,19
4627
PRIMER_RIGHT_16=2557,20
4628
PRIMER_LEFT_16_TM=57.903
4629
PRIMER_RIGHT_16_TM=58.241
4630
PRIMER_LEFT_16_GC_PERCENT=47.368
4631
PRIMER_RIGHT_16_GC_PERCENT=40.000
4632
PRIMER_LEFT_16_SELF_ANY=8.00
4633
PRIMER_RIGHT_16_SELF_ANY=6.00
4634
PRIMER_LEFT_16_SELF_END=1.00
4635
PRIMER_RIGHT_16_SELF_END=3.00
4636
PRIMER_LEFT_16_POSITION_PENALTY=180.000000
4637
PRIMER_RIGHT_16_POSITION_PENALTY=0.500000
4638
PRIMER_LEFT_16_END_STABILITY=6.0000
4639
PRIMER_RIGHT_16_END_STABILITY=8.5000
4640
PRIMER_PAIR_16_COMPL_ANY=5.00
4641
PRIMER_PAIR_16_COMPL_END=3.00
4642
PRIMER_PRODUCT_SIZE_16=2549
4643
PRIMER_PAIR_PENALTY_17=185.3909
4644
PRIMER_LEFT_17_PENALTY=182.631776
4645
PRIMER_RIGHT_17_PENALTY=2.759083
4646
PRIMER_LEFT_17_SEQUENCE=GCAGCTGCAAAGCATCTAATA
4647
PRIMER_RIGHT_17_SEQUENCE=TTACAGCATGCCAAATCCTT
4648
PRIMER_LEFT_17=9,21
4649
PRIMER_RIGHT_17=2558,20
4650
PRIMER_LEFT_17_TM=58.368
4651
PRIMER_RIGHT_17_TM=58.241
4652
PRIMER_LEFT_17_GC_PERCENT=42.857
4653
PRIMER_RIGHT_17_GC_PERCENT=40.000
4654
PRIMER_LEFT_17_SELF_ANY=8.00
4655
PRIMER_RIGHT_17_SELF_ANY=6.00
4656
PRIMER_LEFT_17_SELF_END=3.00
4657
PRIMER_RIGHT_17_SELF_END=1.00
4658
PRIMER_LEFT_17_POSITION_PENALTY=180.000000
4659
PRIMER_RIGHT_17_POSITION_PENALTY=1.000000
4660
PRIMER_LEFT_17_END_STABILITY=5.2000
4661
PRIMER_RIGHT_17_END_STABILITY=8.2000
4662
PRIMER_PAIR_17_COMPL_ANY=6.00
4663
PRIMER_PAIR_17_COMPL_END=2.00
4664
PRIMER_PRODUCT_SIZE_17=2550
4665
PRIMER_PAIR_PENALTY_18=185.4727
4666
PRIMER_LEFT_18_PENALTY=182.631776
4667
PRIMER_RIGHT_18_PENALTY=2.840968
4668
PRIMER_LEFT_18_SEQUENCE=GCAGCTGCAAAGCATCTAATA
4669
PRIMER_RIGHT_18_SEQUENCE=ACAGCATGCCAAATCCTTT
4670
PRIMER_LEFT_18=9,21
4671
PRIMER_RIGHT_18=2556,19
4672
PRIMER_LEFT_18_TM=58.368
4673
PRIMER_RIGHT_18_TM=58.159
4674
PRIMER_LEFT_18_GC_PERCENT=42.857
4675
PRIMER_RIGHT_18_GC_PERCENT=42.105
4676
PRIMER_LEFT_18_SELF_ANY=8.00
4677
PRIMER_RIGHT_18_SELF_ANY=6.00
4678
PRIMER_LEFT_18_SELF_END=3.00
4679
PRIMER_RIGHT_18_SELF_END=3.00
4680
PRIMER_LEFT_18_POSITION_PENALTY=180.000000
4681
PRIMER_RIGHT_18_POSITION_PENALTY=0.000000
4682
PRIMER_LEFT_18_END_STABILITY=5.2000
4683
PRIMER_RIGHT_18_END_STABILITY=8.5000
4684
PRIMER_PAIR_18_COMPL_ANY=5.00
4685
PRIMER_PAIR_18_COMPL_END=3.00
4686
PRIMER_PRODUCT_SIZE_18=2548
4687
PRIMER_PAIR_PENALTY_19=185.4967
4688
PRIMER_LEFT_19_PENALTY=183.096742
4689
PRIMER_RIGHT_19_PENALTY=2.399914
4690
PRIMER_LEFT_19_SEQUENCE=GCAGCTGCAAAGCATCTAA
4691
PRIMER_RIGHT_19_SEQUENCE=TTACAGCATGCCAAATCCTTT
4692
PRIMER_LEFT_19=9,19
4693
PRIMER_RIGHT_19=2558,21
4694
PRIMER_LEFT_19_TM=57.903
4695
PRIMER_RIGHT_19_TM=59.600
4696
PRIMER_LEFT_19_GC_PERCENT=47.368
4697
PRIMER_RIGHT_19_GC_PERCENT=38.095
4698
PRIMER_LEFT_19_SELF_ANY=8.00
4699
PRIMER_RIGHT_19_SELF_ANY=6.00
4700
PRIMER_LEFT_19_SELF_END=1.00
4701
PRIMER_RIGHT_19_SELF_END=3.00
4702
PRIMER_LEFT_19_POSITION_PENALTY=180.000000
4703
PRIMER_RIGHT_19_POSITION_PENALTY=1.000000
4704
PRIMER_LEFT_19_END_STABILITY=6.0000
4705
PRIMER_RIGHT_19_END_STABILITY=8.5000
4706
PRIMER_PAIR_19_COMPL_ANY=6.00
4707
PRIMER_PAIR_19_COMPL_END=3.00
4708
PRIMER_PRODUCT_SIZE_19=2550
4709
=
4710
PRIMER_SEQUENCE_ID=L22005/HCDC34
4711
PRIMER_COMMENT=Human ubiquitin conjugating enzyme mRNA, partial cds. CDS=<1..897
4712
PRIMER_START_CODON_POSITION=-3
4713
SEQUENCE=ATTGCGGCGGCGCCAGAGCTGCTGGAGCGCTCGGGGTCCCCGGGCGGCGGCGGCGGCGCAGAGGAGGAGGCAGGCGGCGGCCCCGGTGGCTCCCCCCCGGACGGTGCGCGGCCCGGCCCGTCTCGCGAACTCGCGGTGGTCGCGCGGCCCCGCGCTGCTCCGACCCCGGGCCCCTCCGCCGCCGCCATGGCTCGGCCGCTAGTGCCCAGCTCGCAGAAGGCGCTGCTGCTGGAGCTCAAGGGGCTGCAGGAAGAGCCGGTCGAGGGATTCCGCGTGACACTGGTGGACGAGGGCGATCTATACAACTGGGAGGTGGCCATTTTCGGGCCCCCCAACACCTACTACGAGGGCGGCTACTTCAAGGCGCGCCTCAAGTTCCCCATCGACTACCCATACTCTCCACCAGCCTTTCGGTTCCTGACCAAGATGTGGCACCCTAACATCTACGAGACGGGGGACGTGTGTATCTCCATCCTCCACCCGCCGGTGGACGACCCCCAGAGCGGGGAGCTGCCCTCAGAGAGGTGGAACCCCACGCAGAACGTCAGGACCATTCTCCTGAGTGTGATCTCCCTCCTGAACGAGCCCAACACCTTCTCGCCCGCAAACGTGGACGCCTCCGTGATGTACAGGAAGTGGAAAGAGAGCAAGGGGAAGGATCGGGAGTACACAGACATCATCCGGAAGCAGGTCCTGGGGACCAAGGTGGACGCGGAGCGTGACGGCGTGAAGGTGCCCACCACGCTGGCCGAGTACTGCGTGAAGACCAAGGCGCCGGCGCCCGACGAGGGCTCAGACCTCTTCTACGACGACTACTACGAGGACGGCGAGGTGGAGGAGGAGGCCGACAGCTGCTTCGGGGACGATGAGGATGACTCTGGCACGGAGGAGTCCTGACACCACCAGAATAAACTTGCCGAGTTTACCTCACTAGGGCCGGACCCGTGGCTCCTTAGACGACAGACTACCTCACGGAGGTTTTGTGCTGGTCCCCGTCTCCTCTGGTTGTTTCGTTTTGGCTTTTTCTCCCTCCCCATGTCTGTTCTGGGTTTTCACGTGCTTCAGAGAAGAGGGGCTGCCCCACCGCCACTCACGTCACTCGGGGCTCGGTGGACGGGCCCAGGGTGGGAGCGGCCGGCCCACCTGTCCCCTCGGGAGGGGAGCTGAGCCCGACTTCTACCGGGGTCCCCCAGCTTCCGGACTGGCCGCACCCCGGAGGAGCCACGGGGGCGCTGCTGGGAACGTGGGCGGGGGGCCGTTTCCTGACACTACCAGCCTGGGAGGCCCAGGTGTAGCGGTCCGAGGGGCCCGGTCCTGCCTGTCAGCTCCAGGTCCTGGAGCCACGTCCAGCACTGAGTGGACGGATTCACCAAT
4714
PRIMER_LEFT_EXPLAIN=considered 11417, would not amplify any of the ORF 8815, GC content failed 428, low tm 104, high tm 1651, high end compl 7, long poly-x seq 18,high 3' stability 24, ok 370
4715
PRIMER_RIGHT_EXPLAIN=considered 11034, GC content failed 1402, low tm 461, high tm 7364, high end compl 5, long poly-x seq 44,high 3' stability 50, ok 1708
4716
PRIMER_PAIR_EXPLAIN=considered 71, high end compl 14, ok 57
4717
PRIMER_STOP_CODON_POSITION=894
4718
PRIMER_PAIR_PENALTY=4027.9177
4719
PRIMER_LEFT_PENALTY=4023.813185
4720
PRIMER_RIGHT_PENALTY=4.104466
4721
PRIMER_LEFT_SEQUENCE=CTAGTGCCCAGCTCGCAGA
4722
PRIMER_RIGHT_SEQUENCE=GGTGTCAGGACTCCTCCGT
4723
PRIMER_LEFT=198,19
4724
PRIMER_RIGHT=900,19
4725
PRIMER_LEFT_TM=62.813
4726
PRIMER_RIGHT_TM=60.104
4727
PRIMER_LEFT_GC_PERCENT=63.158
4728
PRIMER_RIGHT_GC_PERCENT=63.158
4729
PRIMER_LEFT_SELF_ANY=4.00
4730
PRIMER_RIGHT_SELF_ANY=7.00
4731
PRIMER_LEFT_SELF_END=2.00
4732
PRIMER_RIGHT_SELF_END=0.00
4733
PRIMER_LEFT_POSITION_PENALTY=4020.000000
4734
PRIMER_RIGHT_POSITION_PENALTY=3.000000
4735
PRIMER_LEFT_END_STABILITY=8.2000
4736
PRIMER_RIGHT_END_STABILITY=9.6000
4737
PRIMER_PAIR_COMPL_ANY=3.00
4738
PRIMER_PAIR_COMPL_END=1.00
4739
PRIMER_PRODUCT_SIZE=703
4740
PRIMER_PAIR_PENALTY_1=4028.1023
4741
PRIMER_LEFT_1_PENALTY=4023.813185
4742
PRIMER_RIGHT_1_PENALTY=4.289096
4743
PRIMER_LEFT_1_SEQUENCE=CTAGTGCCCAGCTCGCAGA
4744
PRIMER_RIGHT_1_SEQUENCE=GTGTCAGGACTCCTCCGTG
4745
PRIMER_LEFT_1=198,19
4746
PRIMER_RIGHT_1=899,19
4747
PRIMER_LEFT_1_TM=62.813
4748
PRIMER_RIGHT_1_TM=59.211
4749
PRIMER_LEFT_1_GC_PERCENT=63.158
4750
PRIMER_RIGHT_1_GC_PERCENT=63.158
4751
PRIMER_LEFT_1_SELF_ANY=4.00
4752
PRIMER_RIGHT_1_SELF_ANY=7.00
4753
PRIMER_LEFT_1_SELF_END=2.00
4754
PRIMER_RIGHT_1_SELF_END=2.00
4755
PRIMER_LEFT_1_POSITION_PENALTY=4020.000000
4756
PRIMER_RIGHT_1_POSITION_PENALTY=2.500000
4757
PRIMER_LEFT_1_END_STABILITY=8.2000
4758
PRIMER_RIGHT_1_END_STABILITY=9.9000
4759
PRIMER_PAIR_1_COMPL_ANY=3.00
4760
PRIMER_PAIR_1_COMPL_END=2.00
4761
PRIMER_PRODUCT_SIZE_1=702
4762
PRIMER_PAIR_PENALTY_2=4028.1071
4763
PRIMER_LEFT_2_PENALTY=4024.002640
4764
PRIMER_RIGHT_2_PENALTY=4.104466
4765
PRIMER_LEFT_2_SEQUENCE=CTAGTGCCCAGCTCGCAGAA
4766
PRIMER_RIGHT_2_SEQUENCE=GGTGTCAGGACTCCTCCGT
4767
PRIMER_LEFT_2=198,20
4768
PRIMER_RIGHT_2=900,19
4769
PRIMER_LEFT_2_TM=64.003
4770
PRIMER_RIGHT_2_TM=60.104
4771
PRIMER_LEFT_2_GC_PERCENT=60.000
4772
PRIMER_RIGHT_2_GC_PERCENT=63.158
4773
PRIMER_LEFT_2_SELF_ANY=4.00
4774
PRIMER_RIGHT_2_SELF_ANY=7.00
4775
PRIMER_LEFT_2_SELF_END=2.00
4776
PRIMER_RIGHT_2_SELF_END=0.00
4777
PRIMER_LEFT_2_POSITION_PENALTY=4020.000000
4778
PRIMER_RIGHT_2_POSITION_PENALTY=3.000000
4779
PRIMER_LEFT_2_END_STABILITY=7.0000
4780
PRIMER_RIGHT_2_END_STABILITY=9.6000
4781
PRIMER_PAIR_2_COMPL_ANY=3.00
4782
PRIMER_PAIR_2_COMPL_END=2.00
4783
PRIMER_PRODUCT_SIZE_2=703
4784
PRIMER_PAIR_PENALTY_3=4028.1792
4785
PRIMER_LEFT_3_PENALTY=4023.813185
4786
PRIMER_RIGHT_3_PENALTY=4.365996
4787
PRIMER_LEFT_3_SEQUENCE=CTAGTGCCCAGCTCGCAGA
4788
PRIMER_RIGHT_3_SEQUENCE=AGGACTCCTCCGTGCCAGA
4789
PRIMER_LEFT_3=198,19
4790
PRIMER_RIGHT_3=894,19
4791
PRIMER_LEFT_3_TM=62.813
4792
PRIMER_RIGHT_3_TM=63.366
4793
PRIMER_LEFT_3_GC_PERCENT=63.158
4794
PRIMER_RIGHT_3_GC_PERCENT=63.158
4795
PRIMER_LEFT_3_SELF_ANY=4.00
4796
PRIMER_RIGHT_3_SELF_ANY=7.00
4797
PRIMER_LEFT_3_SELF_END=2.00
4798
PRIMER_RIGHT_3_SELF_END=2.00
4799
PRIMER_LEFT_3_POSITION_PENALTY=4020.000000
4800
PRIMER_RIGHT_3_POSITION_PENALTY=0.000000
4801
PRIMER_LEFT_3_END_STABILITY=8.2000
4802
PRIMER_RIGHT_3_END_STABILITY=8.2000
4803
PRIMER_PAIR_3_COMPL_ANY=4.00
4804
PRIMER_PAIR_3_COMPL_END=1.00
4805
PRIMER_PRODUCT_SIZE_3=697
4806
PRIMER_PAIR_PENALTY_4=4028.2917
4807
PRIMER_LEFT_4_PENALTY=4024.002640
4808
PRIMER_RIGHT_4_PENALTY=4.289096
4809
PRIMER_LEFT_4_SEQUENCE=CTAGTGCCCAGCTCGCAGAA
4810
PRIMER_RIGHT_4_SEQUENCE=GTGTCAGGACTCCTCCGTG
4811
PRIMER_LEFT_4=198,20
4812
PRIMER_RIGHT_4=899,19
4813
PRIMER_LEFT_4_TM=64.003
4814
PRIMER_RIGHT_4_TM=59.211
4815
PRIMER_LEFT_4_GC_PERCENT=60.000
4816
PRIMER_RIGHT_4_GC_PERCENT=63.158
4817
PRIMER_LEFT_4_SELF_ANY=4.00
4818
PRIMER_RIGHT_4_SELF_ANY=7.00
4819
PRIMER_LEFT_4_SELF_END=2.00
4820
PRIMER_RIGHT_4_SELF_END=2.00
4821
PRIMER_LEFT_4_POSITION_PENALTY=4020.000000
4822
PRIMER_RIGHT_4_POSITION_PENALTY=2.500000
4823
PRIMER_LEFT_4_END_STABILITY=7.0000
4824
PRIMER_RIGHT_4_END_STABILITY=9.9000
4825
PRIMER_PAIR_4_COMPL_ANY=3.00
4826
PRIMER_PAIR_4_COMPL_END=3.00
4827
PRIMER_PRODUCT_SIZE_4=702
4828
PRIMER_PAIR_PENALTY_5=4028.3686
4829
PRIMER_LEFT_5_PENALTY=4024.002640
4830
PRIMER_RIGHT_5_PENALTY=4.365996
4831
PRIMER_LEFT_5_SEQUENCE=CTAGTGCCCAGCTCGCAGAA
4832
PRIMER_RIGHT_5_SEQUENCE=AGGACTCCTCCGTGCCAGA
4833
PRIMER_LEFT_5=198,20
4834
PRIMER_RIGHT_5=894,19
4835
PRIMER_LEFT_5_TM=64.003
4836
PRIMER_RIGHT_5_TM=63.366
4837
PRIMER_LEFT_5_GC_PERCENT=60.000
4838
PRIMER_RIGHT_5_GC_PERCENT=63.158
4839
PRIMER_LEFT_5_SELF_ANY=4.00
4840
PRIMER_RIGHT_5_SELF_ANY=7.00
4841
PRIMER_LEFT_5_SELF_END=2.00
4842
PRIMER_RIGHT_5_SELF_END=2.00
4843
PRIMER_LEFT_5_POSITION_PENALTY=4020.000000
4844
PRIMER_RIGHT_5_POSITION_PENALTY=0.000000
4845
PRIMER_LEFT_5_END_STABILITY=7.0000
4846
PRIMER_RIGHT_5_END_STABILITY=8.2000
4847
PRIMER_PAIR_5_COMPL_ANY=4.00
4848
PRIMER_PAIR_5_COMPL_END=2.00
4849
PRIMER_PRODUCT_SIZE_5=697
4850
PRIMER_PAIR_PENALTY_6=4029.3023
4851
PRIMER_LEFT_6_PENALTY=4023.813185
4852
PRIMER_RIGHT_6_PENALTY=5.489119
4853
PRIMER_LEFT_6_SEQUENCE=CTAGTGCCCAGCTCGCAGA
4854
PRIMER_RIGHT_6_SEQUENCE=TGTCAGGACTCCTCCGTGC
4855
PRIMER_LEFT_6=198,19
4856
PRIMER_RIGHT_6=898,19
4857
PRIMER_LEFT_6_TM=62.813
4858
PRIMER_RIGHT_6_TM=62.489
4859
PRIMER_LEFT_6_GC_PERCENT=63.158
4860
PRIMER_RIGHT_6_GC_PERCENT=63.158
4861
PRIMER_LEFT_6_SELF_ANY=4.00
4862
PRIMER_RIGHT_6_SELF_ANY=7.00
4863
PRIMER_LEFT_6_SELF_END=2.00
4864
PRIMER_RIGHT_6_SELF_END=2.00
4865
PRIMER_LEFT_6_POSITION_PENALTY=4020.000000
4866
PRIMER_RIGHT_6_POSITION_PENALTY=2.000000
4867
PRIMER_LEFT_6_END_STABILITY=8.2000
4868
PRIMER_RIGHT_6_END_STABILITY=9.9000
4869
PRIMER_PAIR_6_COMPL_ANY=4.00
4870
PRIMER_PAIR_6_COMPL_END=3.00
4871
PRIMER_PRODUCT_SIZE_6=701
4872
PRIMER_PAIR_PENALTY_7=4029.5632
4873
PRIMER_LEFT_7_PENALTY=4023.813185
4874
PRIMER_RIGHT_7_PENALTY=5.750053
4875
PRIMER_LEFT_7_SEQUENCE=CTAGTGCCCAGCTCGCAGA
4876
PRIMER_RIGHT_7_SEQUENCE=GTCAGGACTCCTCCGTGCC
4877
PRIMER_LEFT_7=198,19
4878
PRIMER_RIGHT_7=897,19
4879
PRIMER_LEFT_7_TM=62.813
4880
PRIMER_RIGHT_7_TM=63.250
4881
PRIMER_LEFT_7_GC_PERCENT=63.158
4882
PRIMER_RIGHT_7_GC_PERCENT=68.421
4883
PRIMER_LEFT_7_SELF_ANY=4.00
4884
PRIMER_RIGHT_7_SELF_ANY=7.00
4885
PRIMER_LEFT_7_SELF_END=2.00
4886
PRIMER_RIGHT_7_SELF_END=2.00
4887
PRIMER_LEFT_7_POSITION_PENALTY=4020.000000
4888
PRIMER_RIGHT_7_POSITION_PENALTY=1.500000
4889
PRIMER_LEFT_7_END_STABILITY=8.2000
4890
PRIMER_RIGHT_7_END_STABILITY=9.4000
4891
PRIMER_PAIR_7_COMPL_ANY=4.00
4892
PRIMER_PAIR_7_COMPL_END=2.00
4893
PRIMER_PRODUCT_SIZE_7=700
4894
PRIMER_PAIR_PENALTY_8=4029.5909
4895
PRIMER_LEFT_8_PENALTY=4023.813185
4896
PRIMER_RIGHT_8_PENALTY=5.777694
4897
PRIMER_LEFT_8_SEQUENCE=CTAGTGCCCAGCTCGCAGA
4898
PRIMER_RIGHT_8_SEQUENCE=GTGTCAGGACTCCTCCGTGC
4899
PRIMER_LEFT_8=198,19
4900
PRIMER_RIGHT_8=899,20
4901
PRIMER_LEFT_8_TM=62.813
4902
PRIMER_RIGHT_8_TM=63.278
4903
PRIMER_LEFT_8_GC_PERCENT=63.158
4904
PRIMER_RIGHT_8_GC_PERCENT=65.000
4905
PRIMER_LEFT_8_SELF_ANY=4.00
4906
PRIMER_RIGHT_8_SELF_ANY=7.00
4907
PRIMER_LEFT_8_SELF_END=2.00
4908
PRIMER_RIGHT_8_SELF_END=2.00
4909
PRIMER_LEFT_8_POSITION_PENALTY=4020.000000
4910
PRIMER_RIGHT_8_POSITION_PENALTY=2.500000
4911
PRIMER_LEFT_8_END_STABILITY=8.2000
4912
PRIMER_RIGHT_8_END_STABILITY=9.9000
4913
PRIMER_PAIR_8_COMPL_ANY=4.00
4914
PRIMER_PAIR_8_COMPL_END=3.00
4915
PRIMER_PRODUCT_SIZE_8=702
4916
PRIMER_PAIR_PENALTY_9=4029.6579
4917
PRIMER_LEFT_9_PENALTY=4023.813185
4918
PRIMER_RIGHT_9_PENALTY=5.844698
4919
PRIMER_LEFT_9_SEQUENCE=CTAGTGCCCAGCTCGCAGA
4920
PRIMER_RIGHT_9_SEQUENCE=AGGACTCCTCCGTGCCAGAGT
4921
PRIMER_LEFT_9=198,19
4922
PRIMER_RIGHT_9=894,21
4923
PRIMER_LEFT_9_TM=62.813
4924
PRIMER_RIGHT_9_TM=64.845
4925
PRIMER_LEFT_9_GC_PERCENT=63.158
4926
PRIMER_RIGHT_9_GC_PERCENT=61.905
4927
PRIMER_LEFT_9_SELF_ANY=4.00
4928
PRIMER_RIGHT_9_SELF_ANY=7.00
4929
PRIMER_LEFT_9_SELF_END=2.00
4930
PRIMER_RIGHT_9_SELF_END=2.00
4931
PRIMER_LEFT_9_POSITION_PENALTY=4020.000000
4932
PRIMER_RIGHT_9_POSITION_PENALTY=0.000000
4933
PRIMER_LEFT_9_END_STABILITY=8.2000
4934
PRIMER_RIGHT_9_END_STABILITY=6.1000
4935
PRIMER_PAIR_9_COMPL_ANY=4.00
4936
PRIMER_PAIR_9_COMPL_END=1.00
4937
PRIMER_PRODUCT_SIZE_9=697
4938
PRIMER_PAIR_PENALTY_10=4029.7039
4939
PRIMER_LEFT_10_PENALTY=4023.813185
4940
PRIMER_RIGHT_10_PENALTY=5.890719
4941
PRIMER_LEFT_10_SEQUENCE=CTAGTGCCCAGCTCGCAGA
4942
PRIMER_RIGHT_10_SEQUENCE=CAGGACTCCTCCGTGCCAG
4943
PRIMER_LEFT_10=198,19
4944
PRIMER_RIGHT_10=895,19
4945
PRIMER_LEFT_10_TM=62.813
4946
PRIMER_RIGHT_10_TM=64.391
4947
PRIMER_LEFT_10_GC_PERCENT=63.158
4948
PRIMER_RIGHT_10_GC_PERCENT=68.421
4949
PRIMER_LEFT_10_SELF_ANY=4.00
4950
PRIMER_RIGHT_10_SELF_ANY=7.00
4951
PRIMER_LEFT_10_SELF_END=2.00
4952
PRIMER_RIGHT_10_SELF_END=1.00
4953
PRIMER_LEFT_10_POSITION_PENALTY=4020.000000
4954
PRIMER_RIGHT_10_POSITION_PENALTY=0.500000
4955
PRIMER_LEFT_10_END_STABILITY=8.2000
4956
PRIMER_RIGHT_10_END_STABILITY=9.7000
4957
PRIMER_PAIR_10_COMPL_ANY=4.00
4958
PRIMER_PAIR_10_COMPL_END=2.00
4959
PRIMER_PRODUCT_SIZE_10=698
4960
PRIMER_PAIR_PENALTY_11=4029.7527
4961
PRIMER_LEFT_11_PENALTY=4024.002640
4962
PRIMER_RIGHT_11_PENALTY=5.750053
4963
PRIMER_LEFT_11_SEQUENCE=CTAGTGCCCAGCTCGCAGAA
4964
PRIMER_RIGHT_11_SEQUENCE=GTCAGGACTCCTCCGTGCC
4965
PRIMER_LEFT_11=198,20
4966
PRIMER_RIGHT_11=897,19
4967
PRIMER_LEFT_11_TM=64.003
4968
PRIMER_RIGHT_11_TM=63.250
4969
PRIMER_LEFT_11_GC_PERCENT=60.000
4970
PRIMER_RIGHT_11_GC_PERCENT=68.421
4971
PRIMER_LEFT_11_SELF_ANY=4.00
4972
PRIMER_RIGHT_11_SELF_ANY=7.00
4973
PRIMER_LEFT_11_SELF_END=2.00
4974
PRIMER_RIGHT_11_SELF_END=2.00
4975
PRIMER_LEFT_11_POSITION_PENALTY=4020.000000
4976
PRIMER_RIGHT_11_POSITION_PENALTY=1.500000
4977
PRIMER_LEFT_11_END_STABILITY=7.0000
4978
PRIMER_RIGHT_11_END_STABILITY=9.4000
4979
PRIMER_PAIR_11_COMPL_ANY=4.00
4980
PRIMER_PAIR_11_COMPL_END=3.00
4981
PRIMER_PRODUCT_SIZE_11=700
4982
PRIMER_PAIR_PENALTY_12=4029.8473
4983
PRIMER_LEFT_12_PENALTY=4024.002640
4984
PRIMER_RIGHT_12_PENALTY=5.844698
4985
PRIMER_LEFT_12_SEQUENCE=CTAGTGCCCAGCTCGCAGAA
4986
PRIMER_RIGHT_12_SEQUENCE=AGGACTCCTCCGTGCCAGAGT
4987
PRIMER_LEFT_12=198,20
4988
PRIMER_RIGHT_12=894,21
4989
PRIMER_LEFT_12_TM=64.003
4990
PRIMER_RIGHT_12_TM=64.845
4991
PRIMER_LEFT_12_GC_PERCENT=60.000
4992
PRIMER_RIGHT_12_GC_PERCENT=61.905
4993
PRIMER_LEFT_12_SELF_ANY=4.00
4994
PRIMER_RIGHT_12_SELF_ANY=7.00
4995
PRIMER_LEFT_12_SELF_END=2.00
4996
PRIMER_RIGHT_12_SELF_END=2.00
4997
PRIMER_LEFT_12_POSITION_PENALTY=4020.000000
4998
PRIMER_RIGHT_12_POSITION_PENALTY=0.000000
4999
PRIMER_LEFT_12_END_STABILITY=7.0000
5000
PRIMER_RIGHT_12_END_STABILITY=6.1000
5001
PRIMER_PAIR_12_COMPL_ANY=4.00
5002
PRIMER_PAIR_12_COMPL_END=1.00
5003
PRIMER_PRODUCT_SIZE_12=697
5004
PRIMER_PAIR_PENALTY_13=4029.8934
5005
PRIMER_LEFT_13_PENALTY=4024.002640
5006
PRIMER_RIGHT_13_PENALTY=5.890719
5007
PRIMER_LEFT_13_SEQUENCE=CTAGTGCCCAGCTCGCAGAA
5008
PRIMER_RIGHT_13_SEQUENCE=CAGGACTCCTCCGTGCCAG
5009
PRIMER_LEFT_13=198,20
5010
PRIMER_RIGHT_13=895,19
5011
PRIMER_LEFT_13_TM=64.003
5012
PRIMER_RIGHT_13_TM=64.391
5013
PRIMER_LEFT_13_GC_PERCENT=60.000
5014
PRIMER_RIGHT_13_GC_PERCENT=68.421
5015
PRIMER_LEFT_13_SELF_ANY=4.00
5016
PRIMER_RIGHT_13_SELF_ANY=7.00
5017
PRIMER_LEFT_13_SELF_END=2.00
5018
PRIMER_RIGHT_13_SELF_END=1.00
5019
PRIMER_LEFT_13_POSITION_PENALTY=4020.000000
5020
PRIMER_RIGHT_13_POSITION_PENALTY=0.500000
5021
PRIMER_LEFT_13_END_STABILITY=7.0000
5022
PRIMER_RIGHT_13_END_STABILITY=9.7000
5023
PRIMER_PAIR_13_COMPL_ANY=4.00
5024
PRIMER_PAIR_13_COMPL_END=3.00
5025
PRIMER_PRODUCT_SIZE_13=698
5026
PRIMER_PAIR_PENALTY_14=4029.9325
5027
PRIMER_LEFT_14_PENALTY=4023.813185
5028
PRIMER_RIGHT_14_PENALTY=6.119361
5029
PRIMER_LEFT_14_SEQUENCE=CTAGTGCCCAGCTCGCAGA
5030
PRIMER_RIGHT_14_SEQUENCE=GGTGTCAGGACTCCTCCGTG
5031
PRIMER_LEFT_14=198,19
5032
PRIMER_RIGHT_14=900,20
5033
PRIMER_LEFT_14_TM=62.813
5034
PRIMER_RIGHT_14_TM=63.119
5035
PRIMER_LEFT_14_GC_PERCENT=63.158
5036
PRIMER_RIGHT_14_GC_PERCENT=65.000
5037
PRIMER_LEFT_14_SELF_ANY=4.00
5038
PRIMER_RIGHT_14_SELF_ANY=7.00
5039
PRIMER_LEFT_14_SELF_END=2.00
5040
PRIMER_RIGHT_14_SELF_END=2.00
5041
PRIMER_LEFT_14_POSITION_PENALTY=4020.000000
5042
PRIMER_RIGHT_14_POSITION_PENALTY=3.000000
5043
PRIMER_LEFT_14_END_STABILITY=8.2000
5044
PRIMER_RIGHT_14_END_STABILITY=9.9000
5045
PRIMER_PAIR_14_COMPL_ANY=3.00
5046
PRIMER_PAIR_14_COMPL_END=2.00
5047
PRIMER_PRODUCT_SIZE_14=703
5048
PRIMER_PAIR_PENALTY_15=4030.1220
5049
PRIMER_LEFT_15_PENALTY=4024.002640
5050
PRIMER_RIGHT_15_PENALTY=6.119361
5051
PRIMER_LEFT_15_SEQUENCE=CTAGTGCCCAGCTCGCAGAA
5052
PRIMER_RIGHT_15_SEQUENCE=GGTGTCAGGACTCCTCCGTG
5053
PRIMER_LEFT_15=198,20
5054
PRIMER_RIGHT_15=900,20
5055
PRIMER_LEFT_15_TM=64.003
5056
PRIMER_RIGHT_15_TM=63.119
5057
PRIMER_LEFT_15_GC_PERCENT=60.000
5058
PRIMER_RIGHT_15_GC_PERCENT=65.000
5059
PRIMER_LEFT_15_SELF_ANY=4.00
5060
PRIMER_RIGHT_15_SELF_ANY=7.00
5061
PRIMER_LEFT_15_SELF_END=2.00
5062
PRIMER_RIGHT_15_SELF_END=2.00
5063
PRIMER_LEFT_15_POSITION_PENALTY=4020.000000
5064
PRIMER_RIGHT_15_POSITION_PENALTY=3.000000
5065
PRIMER_LEFT_15_END_STABILITY=7.0000
5066
PRIMER_RIGHT_15_END_STABILITY=9.9000
5067
PRIMER_PAIR_15_COMPL_ANY=3.00
5068
PRIMER_PAIR_15_COMPL_END=3.00
5069
PRIMER_PRODUCT_SIZE_15=703
5070
PRIMER_PAIR_PENALTY_16=4030.4325
5071
PRIMER_LEFT_16_PENALTY=4023.813185
5072
PRIMER_RIGHT_16_PENALTY=6.619361
5073
PRIMER_LEFT_16_SEQUENCE=CTAGTGCCCAGCTCGCAGA
5074
PRIMER_RIGHT_16_SEQUENCE=TGGTGTCAGGACTCCTCCGT
5075
PRIMER_LEFT_16=198,19
5076
PRIMER_RIGHT_16=901,20
5077
PRIMER_LEFT_16_TM=62.813
5078
PRIMER_RIGHT_16_TM=63.119
5079
PRIMER_LEFT_16_GC_PERCENT=63.158
5080
PRIMER_RIGHT_16_GC_PERCENT=60.000
5081
PRIMER_LEFT_16_SELF_ANY=4.00
5082
PRIMER_RIGHT_16_SELF_ANY=7.00
5083
PRIMER_LEFT_16_SELF_END=2.00
5084
PRIMER_RIGHT_16_SELF_END=0.00
5085
PRIMER_LEFT_16_POSITION_PENALTY=4020.000000
5086
PRIMER_RIGHT_16_POSITION_PENALTY=3.500000
5087
PRIMER_LEFT_16_END_STABILITY=8.2000
5088
PRIMER_RIGHT_16_END_STABILITY=9.6000
5089
PRIMER_PAIR_16_COMPL_ANY=3.00
5090
PRIMER_PAIR_16_COMPL_END=1.00
5091
PRIMER_PRODUCT_SIZE_16=704
5092
PRIMER_PAIR_PENALTY_17=4030.6220
5093
PRIMER_LEFT_17_PENALTY=4024.002640
5094
PRIMER_RIGHT_17_PENALTY=6.619361
5095
PRIMER_LEFT_17_SEQUENCE=CTAGTGCCCAGCTCGCAGAA
5096
PRIMER_RIGHT_17_SEQUENCE=TGGTGTCAGGACTCCTCCGT
5097
PRIMER_LEFT_17=198,20
5098
PRIMER_RIGHT_17=901,20
5099
PRIMER_LEFT_17_TM=64.003
5100
PRIMER_RIGHT_17_TM=63.119
5101
PRIMER_LEFT_17_GC_PERCENT=60.000
5102
PRIMER_RIGHT_17_GC_PERCENT=60.000
5103
PRIMER_LEFT_17_SELF_ANY=4.00
5104
PRIMER_RIGHT_17_SELF_ANY=7.00
5105
PRIMER_LEFT_17_SELF_END=2.00
5106
PRIMER_RIGHT_17_SELF_END=0.00
5107
PRIMER_LEFT_17_POSITION_PENALTY=4020.000000
5108
PRIMER_RIGHT_17_POSITION_PENALTY=3.500000
5109
PRIMER_LEFT_17_END_STABILITY=7.0000
5110
PRIMER_RIGHT_17_END_STABILITY=9.6000
5111
PRIMER_PAIR_17_COMPL_ANY=3.00
5112
PRIMER_PAIR_17_COMPL_END=2.00
5113
PRIMER_PRODUCT_SIZE_17=704
5114
PRIMER_PAIR_PENALTY_18=4030.6367
5115
PRIMER_LEFT_18_PENALTY=4023.813185
5116
PRIMER_RIGHT_18_PENALTY=6.823526
5117
PRIMER_LEFT_18_SEQUENCE=CTAGTGCCCAGCTCGCAGA
5118
PRIMER_RIGHT_18_SEQUENCE=TGGTGTCAGGACTCCTCCG
5119
PRIMER_LEFT_18=198,19
5120
PRIMER_RIGHT_18=901,19
5121
PRIMER_LEFT_18_TM=62.813
5122
PRIMER_RIGHT_18_TM=62.324
5123
PRIMER_LEFT_18_GC_PERCENT=63.158
5124
PRIMER_RIGHT_18_GC_PERCENT=63.158
5125
PRIMER_LEFT_18_SELF_ANY=4.00
5126
PRIMER_RIGHT_18_SELF_ANY=7.00
5127
PRIMER_LEFT_18_SELF_END=2.00
5128
PRIMER_RIGHT_18_SELF_END=2.00
5129
PRIMER_LEFT_18_POSITION_PENALTY=4020.000000
5130
PRIMER_RIGHT_18_POSITION_PENALTY=3.500000
5131
PRIMER_LEFT_18_END_STABILITY=8.2000
5132
PRIMER_RIGHT_18_END_STABILITY=9.9000
5133
PRIMER_PAIR_18_COMPL_ANY=3.00
5134
PRIMER_PAIR_18_COMPL_END=2.00
5135
PRIMER_PRODUCT_SIZE_18=704
5136
PRIMER_PAIR_PENALTY_19=4030.8262
5137
PRIMER_LEFT_19_PENALTY=4024.002640
5138
PRIMER_RIGHT_19_PENALTY=6.823526
5139
PRIMER_LEFT_19_SEQUENCE=CTAGTGCCCAGCTCGCAGAA
5140
PRIMER_RIGHT_19_SEQUENCE=TGGTGTCAGGACTCCTCCG
5141
PRIMER_LEFT_19=198,20
5142
PRIMER_RIGHT_19=901,19
5143
PRIMER_LEFT_19_TM=64.003
5144
PRIMER_RIGHT_19_TM=62.324
5145
PRIMER_LEFT_19_GC_PERCENT=60.000
5146
PRIMER_RIGHT_19_GC_PERCENT=63.158
5147
PRIMER_LEFT_19_SELF_ANY=4.00
5148
PRIMER_RIGHT_19_SELF_ANY=7.00
5149
PRIMER_LEFT_19_SELF_END=2.00
5150
PRIMER_RIGHT_19_SELF_END=2.00
5151
PRIMER_LEFT_19_POSITION_PENALTY=4020.000000
5152
PRIMER_RIGHT_19_POSITION_PENALTY=3.500000
5153
PRIMER_LEFT_19_END_STABILITY=7.0000
5154
PRIMER_RIGHT_19_END_STABILITY=9.9000
5155
PRIMER_PAIR_19_COMPL_ANY=3.00
5156
PRIMER_PAIR_19_COMPL_END=3.00
5157
PRIMER_PRODUCT_SIZE_19=704
5158
=
5159
PRIMER_SEQUENCE_ID=M24194/MHC/RACK1
5160
PRIMER_COMMENT=Human MHC protein homologous to chicken B complex protein mRNA, complete cds. CDS=96..1049
5161
PRIMER_START_CODON_POSITION=95
5162
SEQUENCE=CTGCAAGGCGGCGGCAGGAGAGGTTGTGGTGCTAGTTTCTCTAAGCCATCCAGTGCCATCCTCGTCGCTGCAGCGACACCGCTCTCGCCGCCGCCATGACTGAGCAGATGACCCTTCGTGGCACCCTCAAGGGCCACAACGGCTGGGTAACCCAGATCGCTACTACCCCGCAGTTCCCGGACATGATCCTCTCCGCCTCTCGAGATAAGACCATCATCATGTGGAAACTGACCAGGGATGAGACCAACTATGGAATTCCACAGCGTGCTCTGCGGGGTCACTCCCACTTTGTTAGTGATGTGGTTATCTCCTCAGATGGCCAGTTTGCCCTCTCAGGCTCCTGGGATGGAACCCTGCGCCTCTGGGATCTCACAACGGGCACCACCACGAGGCGATTTGTGGGCCATACCAAGGATGTGCTGAGTGTGGCCTTCTCCTCTGACAACCGGCAGATTGTCTCTGGATCTCGAGATAAAACCATCAAGCTATGGAATACCCTGGGTGTGTGCAAATACACTGTCCAGGATGAGAGCCACTCAGAGTGGGTGTCTTGTGTCCGCTTCTCGCCCAACAGCAGCAACCCTATCATCGTCTCCTGTGGCTGGGACAAGCTGGTCAAGGTATGGAACCTGGCTAACTGCAAGCTGAAGACCAACCACATTGGCCACACAGGCTATCTGAACACGGTGACTGTCTCTCCAGATGGATCCCTCTGTGCTTCTGGAGGCAAGGATGGCCAGGCCATGTTATGGGATCTCAACGAAGGCAAACACCTTTACACGCTAGATGGTGGGGACATCATCAACGCCCTGTGCTTCAGCCCTAACCGCTACTGGCTGTGTGCTGCCACAGGCCCCAGCATCAAGATCTGGGATTTAGAGGGAAAGATCATTGTAGATGAACTGAAGCAAGAAGTTATCAGTACCAGCAGCAAGGCAGAACCACCCCAGTGCACTTCCCTGGCCTGGTCTGCTGATGGCCAGACTCTGTTTGCTGGCTACACGGACAACCTGGTGCGAGTGTGGCAGGTGACCATTGGCACACGCTAGAAGTTTATGGCAGAGCTTTACAAATAAAAAAAAAATGGCTTTTC
5163
PRIMER_LEFT_EXPLAIN=considered 8997, would not amplify any of the ORF 6119, GC content failed 1, low tm 301, high tm 1735, high any compl 11, high end compl 8,high 3' stability 24, ok 798
5164
PRIMER_RIGHT_EXPLAIN=considered 8758, GC content failed 64, low tm 957, high tm 5153, high end compl 7, long poly-x seq 16,high 3' stability 45, ok 2516
5165
PRIMER_PAIR_EXPLAIN=considered 82, high end compl 16, ok 66
5166
PRIMER_STOP_CODON_POSITION=1046
5167
PRIMER_PAIR_PENALTY=5.4381
5168
PRIMER_LEFT_PENALTY=2.658657
5169
PRIMER_RIGHT_PENALTY=2.779412
5170
PRIMER_LEFT_SEQUENCE=ATGACTGAGCAGATGACCCTTC
5171
PRIMER_RIGHT_SEQUENCE=TCTAGCGTGTGCCAATGGT
5172
PRIMER_LEFT=95,22
5173
PRIMER_RIGHT=1049,19
5174
PRIMER_LEFT_TM=60.659
5175
PRIMER_RIGHT_TM=60.279
5176
PRIMER_LEFT_GC_PERCENT=50.000
5177
PRIMER_RIGHT_GC_PERCENT=52.632
5178
PRIMER_LEFT_SELF_ANY=4.00
5179
PRIMER_RIGHT_SELF_ANY=5.00
5180
PRIMER_LEFT_SELF_END=1.00
5181
PRIMER_RIGHT_SELF_END=3.00
5182
PRIMER_LEFT_POSITION_PENALTY=0.000000
5183
PRIMER_RIGHT_POSITION_PENALTY=1.500000
5184
PRIMER_LEFT_END_STABILITY=8.2000
5185
PRIMER_RIGHT_END_STABILITY=7.8000
5186
PRIMER_PAIR_COMPL_ANY=4.00
5187
PRIMER_PAIR_COMPL_END=3.00
5188
PRIMER_PRODUCT_SIZE=955
5189
PRIMER_PAIR_PENALTY_1=5.5682
5190
PRIMER_LEFT_1_PENALTY=2.788786
5191
PRIMER_RIGHT_1_PENALTY=2.779412
5192
PRIMER_LEFT_1_SEQUENCE=ATGACTGAGCAGATGACCCT
5193
PRIMER_RIGHT_1_SEQUENCE=TCTAGCGTGTGCCAATGGT
5194
PRIMER_LEFT_1=95,20
5195
PRIMER_RIGHT_1=1049,19
5196
PRIMER_LEFT_1_TM=57.211
5197
PRIMER_RIGHT_1_TM=60.279
5198
PRIMER_LEFT_1_GC_PERCENT=50.000
5199
PRIMER_RIGHT_1_GC_PERCENT=52.632
5200
PRIMER_LEFT_1_SELF_ANY=4.00
5201
PRIMER_RIGHT_1_SELF_ANY=5.00
5202
PRIMER_LEFT_1_SELF_END=0.00
5203
PRIMER_RIGHT_1_SELF_END=3.00
5204
PRIMER_LEFT_1_POSITION_PENALTY=0.000000
5205
PRIMER_RIGHT_1_POSITION_PENALTY=1.500000
5206
PRIMER_LEFT_1_END_STABILITY=9.1000
5207
PRIMER_RIGHT_1_END_STABILITY=7.8000
5208
PRIMER_PAIR_1_COMPL_ANY=3.00
5209
PRIMER_PAIR_1_COMPL_END=3.00
5210
PRIMER_PRODUCT_SIZE_1=955
5211
PRIMER_PAIR_PENALTY_2=6.2893
5212
PRIMER_LEFT_2_PENALTY=2.658657
5213
PRIMER_RIGHT_2_PENALTY=3.630671
5214
PRIMER_LEFT_2_SEQUENCE=ATGACTGAGCAGATGACCCTTC
5215
PRIMER_RIGHT_2_SEQUENCE=TTCTAGCGTGTGCCAATGGT
5216
PRIMER_LEFT_2=95,22
5217
PRIMER_RIGHT_2=1050,20
5218
PRIMER_LEFT_2_TM=60.659
5219
PRIMER_RIGHT_2_TM=61.631
5220
PRIMER_LEFT_2_GC_PERCENT=50.000
5221
PRIMER_RIGHT_2_GC_PERCENT=50.000
5222
PRIMER_LEFT_2_SELF_ANY=4.00
5223
PRIMER_RIGHT_2_SELF_ANY=5.00
5224
PRIMER_LEFT_2_SELF_END=1.00
5225
PRIMER_RIGHT_2_SELF_END=3.00
5226
PRIMER_LEFT_2_POSITION_PENALTY=0.000000
5227
PRIMER_RIGHT_2_POSITION_PENALTY=2.000000
5228
PRIMER_LEFT_2_END_STABILITY=8.2000
5229
PRIMER_RIGHT_2_END_STABILITY=7.8000
5230
PRIMER_PAIR_2_COMPL_ANY=4.00
5231
PRIMER_PAIR_2_COMPL_END=3.00
5232
PRIMER_PRODUCT_SIZE_2=956
5233
PRIMER_PAIR_PENALTY_3=6.2897
5234
PRIMER_LEFT_3_PENALTY=2.241606
5235
PRIMER_RIGHT_3_PENALTY=4.048100
5236
PRIMER_LEFT_3_SEQUENCE=ATGACTGAGCAGATGACCCTT
5237
PRIMER_RIGHT_3_SEQUENCE=ACTTCTAGCGTGTGCCAATG
5238
PRIMER_LEFT_3=95,21
5239
PRIMER_RIGHT_3=1052,20
5240
PRIMER_LEFT_3_TM=58.758
5241
PRIMER_RIGHT_3_TM=58.952
5242
PRIMER_LEFT_3_GC_PERCENT=47.619
5243
PRIMER_RIGHT_3_GC_PERCENT=50.000
5244
PRIMER_LEFT_3_SELF_ANY=4.00
5245
PRIMER_RIGHT_3_SELF_ANY=4.00
5246
PRIMER_LEFT_3_SELF_END=0.00
5247
PRIMER_RIGHT_3_SELF_END=3.00
5248
PRIMER_LEFT_3_POSITION_PENALTY=0.000000
5249
PRIMER_RIGHT_3_POSITION_PENALTY=3.000000
5250
PRIMER_LEFT_3_END_STABILITY=9.7000
5251
PRIMER_RIGHT_3_END_STABILITY=7.2000
5252
PRIMER_PAIR_3_COMPL_ANY=3.00
5253
PRIMER_PAIR_3_COMPL_END=2.00
5254
PRIMER_PRODUCT_SIZE_3=958
5255
PRIMER_PAIR_PENALTY_4=6.4195
5256
PRIMER_LEFT_4_PENALTY=2.788786
5257
PRIMER_RIGHT_4_PENALTY=3.630671
5258
PRIMER_LEFT_4_SEQUENCE=ATGACTGAGCAGATGACCCT
5259
PRIMER_RIGHT_4_SEQUENCE=TTCTAGCGTGTGCCAATGGT
5260
PRIMER_LEFT_4=95,20
5261
PRIMER_RIGHT_4=1050,20
5262
PRIMER_LEFT_4_TM=57.211
5263
PRIMER_RIGHT_4_TM=61.631
5264
PRIMER_LEFT_4_GC_PERCENT=50.000
5265
PRIMER_RIGHT_4_GC_PERCENT=50.000
5266
PRIMER_LEFT_4_SELF_ANY=4.00
5267
PRIMER_RIGHT_4_SELF_ANY=5.00
5268
PRIMER_LEFT_4_SELF_END=0.00
5269
PRIMER_RIGHT_4_SELF_END=3.00
5270
PRIMER_LEFT_4_POSITION_PENALTY=0.000000
5271
PRIMER_RIGHT_4_POSITION_PENALTY=2.000000
5272
PRIMER_LEFT_4_END_STABILITY=9.1000
5273
PRIMER_RIGHT_4_END_STABILITY=7.8000
5274
PRIMER_PAIR_4_COMPL_ANY=3.00
5275
PRIMER_PAIR_4_COMPL_END=3.00
5276
PRIMER_PRODUCT_SIZE_4=956
5277
PRIMER_PAIR_PENALTY_5=6.7068
5278
PRIMER_LEFT_5_PENALTY=2.658657
5279
PRIMER_RIGHT_5_PENALTY=4.048100
5280
PRIMER_LEFT_5_SEQUENCE=ATGACTGAGCAGATGACCCTTC
5281
PRIMER_RIGHT_5_SEQUENCE=ACTTCTAGCGTGTGCCAATG
5282
PRIMER_LEFT_5=95,22
5283
PRIMER_RIGHT_5=1052,20
5284
PRIMER_LEFT_5_TM=60.659
5285
PRIMER_RIGHT_5_TM=58.952
5286
PRIMER_LEFT_5_GC_PERCENT=50.000
5287
PRIMER_RIGHT_5_GC_PERCENT=50.000
5288
PRIMER_LEFT_5_SELF_ANY=4.00
5289
PRIMER_RIGHT_5_SELF_ANY=4.00
5290
PRIMER_LEFT_5_SELF_END=1.00
5291
PRIMER_RIGHT_5_SELF_END=3.00
5292
PRIMER_LEFT_5_POSITION_PENALTY=0.000000
5293
PRIMER_RIGHT_5_POSITION_PENALTY=3.000000
5294
PRIMER_LEFT_5_END_STABILITY=8.2000
5295
PRIMER_RIGHT_5_END_STABILITY=7.2000
5296
PRIMER_PAIR_5_COMPL_ANY=3.00
5297
PRIMER_PAIR_5_COMPL_END=1.00
5298
PRIMER_PRODUCT_SIZE_5=958
5299
PRIMER_PAIR_PENALTY_6=6.8369
5300
PRIMER_LEFT_6_PENALTY=2.788786
5301
PRIMER_RIGHT_6_PENALTY=4.048100
5302
PRIMER_LEFT_6_SEQUENCE=ATGACTGAGCAGATGACCCT
5303
PRIMER_RIGHT_6_SEQUENCE=ACTTCTAGCGTGTGCCAATG
5304
PRIMER_LEFT_6=95,20
5305
PRIMER_RIGHT_6=1052,20
5306
PRIMER_LEFT_6_TM=57.211
5307
PRIMER_RIGHT_6_TM=58.952
5308
PRIMER_LEFT_6_GC_PERCENT=50.000
5309
PRIMER_RIGHT_6_GC_PERCENT=50.000
5310
PRIMER_LEFT_6_SELF_ANY=4.00
5311
PRIMER_RIGHT_6_SELF_ANY=4.00
5312
PRIMER_LEFT_6_SELF_END=0.00
5313
PRIMER_RIGHT_6_SELF_END=3.00
5314
PRIMER_LEFT_6_POSITION_PENALTY=0.000000
5315
PRIMER_RIGHT_6_POSITION_PENALTY=3.000000
5316
PRIMER_LEFT_6_END_STABILITY=9.1000
5317
PRIMER_RIGHT_6_END_STABILITY=7.2000
5318
PRIMER_PAIR_6_COMPL_ANY=3.00
5319
PRIMER_PAIR_6_COMPL_END=1.00
5320
PRIMER_PRODUCT_SIZE_6=958
5321
PRIMER_PAIR_PENALTY_7=7.0610
5322
PRIMER_LEFT_7_PENALTY=2.241606
5323
PRIMER_RIGHT_7_PENALTY=4.819419
5324
PRIMER_LEFT_7_SEQUENCE=ATGACTGAGCAGATGACCCTT
5325
PRIMER_RIGHT_7_SEQUENCE=AACTTCTAGCGTGTGCCAATG
5326
PRIMER_LEFT_7=95,21
5327
PRIMER_RIGHT_7=1053,21
5328
PRIMER_LEFT_7_TM=58.758
5329
PRIMER_RIGHT_7_TM=60.319
5330
PRIMER_LEFT_7_GC_PERCENT=47.619
5331
PRIMER_RIGHT_7_GC_PERCENT=47.619
5332
PRIMER_LEFT_7_SELF_ANY=4.00
5333
PRIMER_RIGHT_7_SELF_ANY=4.00
5334
PRIMER_LEFT_7_SELF_END=0.00
5335
PRIMER_RIGHT_7_SELF_END=3.00
5336
PRIMER_LEFT_7_POSITION_PENALTY=0.000000
5337
PRIMER_RIGHT_7_POSITION_PENALTY=3.500000
5338
PRIMER_LEFT_7_END_STABILITY=9.7000
5339
PRIMER_RIGHT_7_END_STABILITY=7.2000
5340
PRIMER_PAIR_7_COMPL_ANY=3.00
5341
PRIMER_PAIR_7_COMPL_END=2.00
5342
PRIMER_PRODUCT_SIZE_7=959
5343
PRIMER_PAIR_PENALTY_8=7.4781
5344
PRIMER_LEFT_8_PENALTY=2.658657
5345
PRIMER_RIGHT_8_PENALTY=4.819419
5346
PRIMER_LEFT_8_SEQUENCE=ATGACTGAGCAGATGACCCTTC
5347
PRIMER_RIGHT_8_SEQUENCE=AACTTCTAGCGTGTGCCAATG
5348
PRIMER_LEFT_8=95,22
5349
PRIMER_RIGHT_8=1053,21
5350
PRIMER_LEFT_8_TM=60.659
5351
PRIMER_RIGHT_8_TM=60.319
5352
PRIMER_LEFT_8_GC_PERCENT=50.000
5353
PRIMER_RIGHT_8_GC_PERCENT=47.619
5354
PRIMER_LEFT_8_SELF_ANY=4.00
5355
PRIMER_RIGHT_8_SELF_ANY=4.00
5356
PRIMER_LEFT_8_SELF_END=1.00
5357
PRIMER_RIGHT_8_SELF_END=3.00
5358
PRIMER_LEFT_8_POSITION_PENALTY=0.000000
5359
PRIMER_RIGHT_8_POSITION_PENALTY=3.500000
5360
PRIMER_LEFT_8_END_STABILITY=8.2000
5361
PRIMER_RIGHT_8_END_STABILITY=7.2000
5362
PRIMER_PAIR_8_COMPL_ANY=3.00
5363
PRIMER_PAIR_8_COMPL_END=1.00
5364
PRIMER_PRODUCT_SIZE_8=959
5365
PRIMER_PAIR_PENALTY_9=7.6082
5366
PRIMER_LEFT_9_PENALTY=2.788786
5367
PRIMER_RIGHT_9_PENALTY=4.819419
5368
PRIMER_LEFT_9_SEQUENCE=ATGACTGAGCAGATGACCCT
5369
PRIMER_RIGHT_9_SEQUENCE=AACTTCTAGCGTGTGCCAATG
5370
PRIMER_LEFT_9=95,20
5371
PRIMER_RIGHT_9=1053,21
5372
PRIMER_LEFT_9_TM=57.211
5373
PRIMER_RIGHT_9_TM=60.319
5374
PRIMER_LEFT_9_GC_PERCENT=50.000
5375
PRIMER_RIGHT_9_GC_PERCENT=47.619
5376
PRIMER_LEFT_9_SELF_ANY=4.00
5377
PRIMER_RIGHT_9_SELF_ANY=4.00
5378
PRIMER_LEFT_9_SELF_END=0.00
5379
PRIMER_RIGHT_9_SELF_END=3.00
5380
PRIMER_LEFT_9_POSITION_PENALTY=0.000000
5381
PRIMER_RIGHT_9_POSITION_PENALTY=3.500000
5382
PRIMER_LEFT_9_END_STABILITY=9.1000
5383
PRIMER_RIGHT_9_END_STABILITY=7.2000
5384
PRIMER_PAIR_9_COMPL_ANY=3.00
5385
PRIMER_PAIR_9_COMPL_END=1.00
5386
PRIMER_PRODUCT_SIZE_9=959
5387
PRIMER_PAIR_PENALTY_10=7.6615
5388
PRIMER_LEFT_10_PENALTY=2.241606
5389
PRIMER_RIGHT_10_PENALTY=5.419920
5390
PRIMER_LEFT_10_SEQUENCE=ATGACTGAGCAGATGACCCTT
5391
PRIMER_RIGHT_10_SEQUENCE=AAACTTCTAGCGTGTGCCAA
5392
PRIMER_LEFT_10=95,21
5393
PRIMER_RIGHT_10=1054,20
5394
PRIMER_LEFT_10_TM=58.758
5395
PRIMER_RIGHT_10_TM=58.580
5396
PRIMER_LEFT_10_GC_PERCENT=47.619
5397
PRIMER_RIGHT_10_GC_PERCENT=45.000
5398
PRIMER_LEFT_10_SELF_ANY=4.00
5399
PRIMER_RIGHT_10_SELF_ANY=4.00
5400
PRIMER_LEFT_10_SELF_END=0.00
5401
PRIMER_RIGHT_10_SELF_END=1.00
5402
PRIMER_LEFT_10_POSITION_PENALTY=0.000000
5403
PRIMER_RIGHT_10_POSITION_PENALTY=4.000000
5404
PRIMER_LEFT_10_END_STABILITY=9.7000
5405
PRIMER_RIGHT_10_END_STABILITY=10.0000
5406
PRIMER_PAIR_10_COMPL_ANY=3.00
5407
PRIMER_PAIR_10_COMPL_END=2.00
5408
PRIMER_PRODUCT_SIZE_10=960
5409
PRIMER_PAIR_PENALTY_11=7.7289
5410
PRIMER_LEFT_11_PENALTY=2.241606
5411
PRIMER_RIGHT_11_PENALTY=5.487331
5412
PRIMER_LEFT_11_SEQUENCE=ATGACTGAGCAGATGACCCTT
5413
PRIMER_RIGHT_11_SEQUENCE=CTTCTAGCGTGTGCCAATG
5414
PRIMER_LEFT_11=95,21
5415
PRIMER_RIGHT_11=1051,19
5416
PRIMER_LEFT_11_TM=58.758
5417
PRIMER_RIGHT_11_TM=58.013
5418
PRIMER_LEFT_11_GC_PERCENT=47.619
5419
PRIMER_RIGHT_11_GC_PERCENT=52.632
5420
PRIMER_LEFT_11_SELF_ANY=4.00
5421
PRIMER_RIGHT_11_SELF_ANY=4.00
5422
PRIMER_LEFT_11_SELF_END=0.00
5423
PRIMER_RIGHT_11_SELF_END=3.00
5424
PRIMER_LEFT_11_POSITION_PENALTY=0.000000
5425
PRIMER_RIGHT_11_POSITION_PENALTY=2.500000
5426
PRIMER_LEFT_11_END_STABILITY=9.7000
5427
PRIMER_RIGHT_11_END_STABILITY=7.2000
5428
PRIMER_PAIR_11_COMPL_ANY=3.00
5429
PRIMER_PAIR_11_COMPL_END=2.00
5430
PRIMER_PRODUCT_SIZE_11=957
5431
PRIMER_PAIR_PENALTY_12=8.0786
5432
PRIMER_LEFT_12_PENALTY=2.658657
5433
PRIMER_RIGHT_12_PENALTY=5.419920
5434
PRIMER_LEFT_12_SEQUENCE=ATGACTGAGCAGATGACCCTTC
5435
PRIMER_RIGHT_12_SEQUENCE=AAACTTCTAGCGTGTGCCAA
5436
PRIMER_LEFT_12=95,22
5437
PRIMER_RIGHT_12=1054,20
5438
PRIMER_LEFT_12_TM=60.659
5439
PRIMER_RIGHT_12_TM=58.580
5440
PRIMER_LEFT_12_GC_PERCENT=50.000
5441
PRIMER_RIGHT_12_GC_PERCENT=45.000
5442
PRIMER_LEFT_12_SELF_ANY=4.00
5443
PRIMER_RIGHT_12_SELF_ANY=4.00
5444
PRIMER_LEFT_12_SELF_END=1.00
5445
PRIMER_RIGHT_12_SELF_END=1.00
5446
PRIMER_LEFT_12_POSITION_PENALTY=0.000000
5447
PRIMER_RIGHT_12_POSITION_PENALTY=4.000000
5448
PRIMER_LEFT_12_END_STABILITY=8.2000
5449
PRIMER_RIGHT_12_END_STABILITY=10.0000
5450
PRIMER_PAIR_12_COMPL_ANY=3.00
5451
PRIMER_PAIR_12_COMPL_END=1.00
5452
PRIMER_PRODUCT_SIZE_12=960
5453
PRIMER_PAIR_PENALTY_13=8.1460
5454
PRIMER_LEFT_13_PENALTY=2.658657
5455
PRIMER_RIGHT_13_PENALTY=5.487331
5456
PRIMER_LEFT_13_SEQUENCE=ATGACTGAGCAGATGACCCTTC
5457
PRIMER_RIGHT_13_SEQUENCE=CTTCTAGCGTGTGCCAATG
5458
PRIMER_LEFT_13=95,22
5459
PRIMER_RIGHT_13=1051,19
5460
PRIMER_LEFT_13_TM=60.659
5461
PRIMER_RIGHT_13_TM=58.013
5462
PRIMER_LEFT_13_GC_PERCENT=50.000
5463
PRIMER_RIGHT_13_GC_PERCENT=52.632
5464
PRIMER_LEFT_13_SELF_ANY=4.00
5465
PRIMER_RIGHT_13_SELF_ANY=4.00
5466
PRIMER_LEFT_13_SELF_END=1.00
5467
PRIMER_RIGHT_13_SELF_END=3.00
5468
PRIMER_LEFT_13_POSITION_PENALTY=0.000000
5469
PRIMER_RIGHT_13_POSITION_PENALTY=2.500000
5470
PRIMER_LEFT_13_END_STABILITY=8.2000
5471
PRIMER_RIGHT_13_END_STABILITY=7.2000
5472
PRIMER_PAIR_13_COMPL_ANY=3.00
5473
PRIMER_PAIR_13_COMPL_END=1.00
5474
PRIMER_PRODUCT_SIZE_13=957
5475
PRIMER_PAIR_PENALTY_14=8.2087
5476
PRIMER_LEFT_14_PENALTY=2.788786
5477
PRIMER_RIGHT_14_PENALTY=5.419920
5478
PRIMER_LEFT_14_SEQUENCE=ATGACTGAGCAGATGACCCT
5479
PRIMER_RIGHT_14_SEQUENCE=AAACTTCTAGCGTGTGCCAA
5480
PRIMER_LEFT_14=95,20
5481
PRIMER_RIGHT_14=1054,20
5482
PRIMER_LEFT_14_TM=57.211
5483
PRIMER_RIGHT_14_TM=58.580
5484
PRIMER_LEFT_14_GC_PERCENT=50.000
5485
PRIMER_RIGHT_14_GC_PERCENT=45.000
5486
PRIMER_LEFT_14_SELF_ANY=4.00
5487
PRIMER_RIGHT_14_SELF_ANY=4.00
5488
PRIMER_LEFT_14_SELF_END=0.00
5489
PRIMER_RIGHT_14_SELF_END=1.00
5490
PRIMER_LEFT_14_POSITION_PENALTY=0.000000
5491
PRIMER_RIGHT_14_POSITION_PENALTY=4.000000
5492
PRIMER_LEFT_14_END_STABILITY=9.1000
5493
PRIMER_RIGHT_14_END_STABILITY=10.0000
5494
PRIMER_PAIR_14_COMPL_ANY=3.00
5495
PRIMER_PAIR_14_COMPL_END=1.00
5496
PRIMER_PRODUCT_SIZE_14=960
5497
PRIMER_PAIR_PENALTY_15=8.2320
5498
PRIMER_LEFT_15_PENALTY=2.241606
5499
PRIMER_RIGHT_15_PENALTY=5.990437
5500
PRIMER_LEFT_15_SEQUENCE=ATGACTGAGCAGATGACCCTT
5501
PRIMER_RIGHT_15_SEQUENCE=AACTTCTAGCGTGTGCCAAT
5502
PRIMER_LEFT_15=95,21
5503
PRIMER_RIGHT_15=1053,20
5504
PRIMER_LEFT_15_TM=58.758
5505
PRIMER_RIGHT_15_TM=57.510
5506
PRIMER_LEFT_15_GC_PERCENT=47.619
5507
PRIMER_RIGHT_15_GC_PERCENT=45.000
5508
PRIMER_LEFT_15_SELF_ANY=4.00
5509
PRIMER_RIGHT_15_SELF_ANY=4.00
5510
PRIMER_LEFT_15_SELF_END=0.00
5511
PRIMER_RIGHT_15_SELF_END=2.00
5512
PRIMER_LEFT_15_POSITION_PENALTY=0.000000
5513
PRIMER_RIGHT_15_POSITION_PENALTY=3.500000
5514
PRIMER_LEFT_15_END_STABILITY=9.7000
5515
PRIMER_RIGHT_15_END_STABILITY=8.4000
5516
PRIMER_PAIR_15_COMPL_ANY=3.00
5517
PRIMER_PAIR_15_COMPL_END=1.00
5518
PRIMER_PRODUCT_SIZE_15=959
5519
PRIMER_PAIR_PENALTY_16=8.2761
5520
PRIMER_LEFT_16_PENALTY=2.788786
5521
PRIMER_RIGHT_16_PENALTY=5.487331
5522
PRIMER_LEFT_16_SEQUENCE=ATGACTGAGCAGATGACCCT
5523
PRIMER_RIGHT_16_SEQUENCE=CTTCTAGCGTGTGCCAATG
5524
PRIMER_LEFT_16=95,20
5525
PRIMER_RIGHT_16=1051,19
5526
PRIMER_LEFT_16_TM=57.211
5527
PRIMER_RIGHT_16_TM=58.013
5528
PRIMER_LEFT_16_GC_PERCENT=50.000
5529
PRIMER_RIGHT_16_GC_PERCENT=52.632
5530
PRIMER_LEFT_16_SELF_ANY=4.00
5531
PRIMER_RIGHT_16_SELF_ANY=4.00
5532
PRIMER_LEFT_16_SELF_END=0.00
5533
PRIMER_RIGHT_16_SELF_END=3.00
5534
PRIMER_LEFT_16_POSITION_PENALTY=0.000000
5535
PRIMER_RIGHT_16_POSITION_PENALTY=2.500000
5536
PRIMER_LEFT_16_END_STABILITY=9.1000
5537
PRIMER_RIGHT_16_END_STABILITY=7.2000
5538
PRIMER_PAIR_16_COMPL_ANY=3.00
5539
PRIMER_PAIR_16_COMPL_END=1.00
5540
PRIMER_PRODUCT_SIZE_16=957
5541
PRIMER_PAIR_PENALTY_17=8.3221
5542
PRIMER_LEFT_17_PENALTY=2.241606
5543
PRIMER_RIGHT_17_PENALTY=6.080486
5544
PRIMER_LEFT_17_SEQUENCE=ATGACTGAGCAGATGACCCTT
5545
PRIMER_RIGHT_17_SEQUENCE=AAACTTCTAGCGTGTGCCAAT
5546
PRIMER_LEFT_17=95,21
5547
PRIMER_RIGHT_17=1054,21
5548
PRIMER_LEFT_17_TM=58.758
5549
PRIMER_RIGHT_17_TM=58.920
5550
PRIMER_LEFT_17_GC_PERCENT=47.619
5551
PRIMER_RIGHT_17_GC_PERCENT=42.857
5552
PRIMER_LEFT_17_SELF_ANY=4.00
5553
PRIMER_RIGHT_17_SELF_ANY=4.00
5554
PRIMER_LEFT_17_SELF_END=0.00
5555
PRIMER_RIGHT_17_SELF_END=2.00
5556
PRIMER_LEFT_17_POSITION_PENALTY=0.000000
5557
PRIMER_RIGHT_17_POSITION_PENALTY=4.000000
5558
PRIMER_LEFT_17_END_STABILITY=9.7000
5559
PRIMER_RIGHT_17_END_STABILITY=8.4000
5560
PRIMER_PAIR_17_COMPL_ANY=3.00
5561
PRIMER_PAIR_17_COMPL_END=1.00
5562
PRIMER_PRODUCT_SIZE_17=960
5563
PRIMER_PAIR_PENALTY_18=8.6491
5564
PRIMER_LEFT_18_PENALTY=2.658657
5565
PRIMER_RIGHT_18_PENALTY=5.990437
5566
PRIMER_LEFT_18_SEQUENCE=ATGACTGAGCAGATGACCCTTC
5567
PRIMER_RIGHT_18_SEQUENCE=AACTTCTAGCGTGTGCCAAT
5568
PRIMER_LEFT_18=95,22
5569
PRIMER_RIGHT_18=1053,20
5570
PRIMER_LEFT_18_TM=60.659
5571
PRIMER_RIGHT_18_TM=57.510
5572
PRIMER_LEFT_18_GC_PERCENT=50.000
5573
PRIMER_RIGHT_18_GC_PERCENT=45.000
5574
PRIMER_LEFT_18_SELF_ANY=4.00
5575
PRIMER_RIGHT_18_SELF_ANY=4.00
5576
PRIMER_LEFT_18_SELF_END=1.00
5577
PRIMER_RIGHT_18_SELF_END=2.00
5578
PRIMER_LEFT_18_POSITION_PENALTY=0.000000
5579
PRIMER_RIGHT_18_POSITION_PENALTY=3.500000
5580
PRIMER_LEFT_18_END_STABILITY=8.2000
5581
PRIMER_RIGHT_18_END_STABILITY=8.4000
5582
PRIMER_PAIR_18_COMPL_ANY=3.00
5583
PRIMER_PAIR_18_COMPL_END=0.00
5584
PRIMER_PRODUCT_SIZE_18=959
5585
PRIMER_PAIR_PENALTY_19=8.6509
5586
PRIMER_LEFT_19_PENALTY=2.658657
5587
PRIMER_RIGHT_19_PENALTY=5.992277
5588
PRIMER_LEFT_19_SEQUENCE=ATGACTGAGCAGATGACCCTTC
5589
PRIMER_RIGHT_19_SEQUENCE=CTTCTAGCGTGTGCCAATGGT
5590
PRIMER_LEFT_19=95,22
5591
PRIMER_RIGHT_19=1051,21
5592
PRIMER_LEFT_19_TM=60.659
5593
PRIMER_RIGHT_19_TM=62.492
5594
PRIMER_LEFT_19_GC_PERCENT=50.000
5595
PRIMER_RIGHT_19_GC_PERCENT=52.381
5596
PRIMER_LEFT_19_SELF_ANY=4.00
5597
PRIMER_RIGHT_19_SELF_ANY=5.00
5598
PRIMER_LEFT_19_SELF_END=1.00
5599
PRIMER_RIGHT_19_SELF_END=3.00
5600
PRIMER_LEFT_19_POSITION_PENALTY=0.000000
5601
PRIMER_RIGHT_19_POSITION_PENALTY=2.500000
5602
PRIMER_LEFT_19_END_STABILITY=8.2000
5603
PRIMER_RIGHT_19_END_STABILITY=7.8000
5604
PRIMER_PAIR_19_COMPL_ANY=4.00
5605
PRIMER_PAIR_19_COMPL_END=3.00
5606
PRIMER_PRODUCT_SIZE_19=957
5607
=
5608
PRIMER_SEQUENCE_ID=X66357/CDK3
5609
PRIMER_COMMENT=H.sapiens mRNA cdk3 for serine/threonine protein kinase. CDS=89..1006
5610
PRIMER_START_CODON_POSITION=88
5611
SEQUENCE=CCACATGGAAGCTGGAGGAGCAACCGGGAGCGCTGGGCTGGGGTGCAAATTGCCCAGTGCCTTCTGTTTCCCAGGCAGCTCTGTGGCCATGGATATGTTCCAGAAGGTAGAGAAGATCGGAGAGGGCACCTATGGGGTGGTGTACAAGGCCAAGAACAGGGAGACAGGGCAGCTGGTGGCCCTGAAGAAGATCAGACTGGATTTGGAGATGGAGGGGGTCCCAAGCACTGCCATCAGGGAGATCTCGCTGCTCAAGGAACTGAAGCACCCCAACATCGTCCGACTGCTGGACGTGGTGCACAACGAGAGGAAGCTCTATCTGGTGTTTGAGTTCCTCAGCCAGGACCTGAAGAAGTACATGGACTCCACCCCAGGCTCAGAGCTCCCCCTGCACCTCATCAAGAGCTACCTCTTCCAGCTGCTGCAGGGGGTGAGTTTCTGCCACTCACATCGGGTCATCCACCGAGACCTGAAGCCCCAGAACCTGCTCATCAATGAGTTGGGTGCCATCAAGCTGGCTGACTTCGGCCTGGCTCGCGCCTTCGGGGTGCCCCTGCGCACCTACACCCATGAGGTGGTGACACTGTGGTATCGCGCCCCCGAGATTCTCTTGGGCAGCAAGTTCTATACCACAGCTGTGGATATCTGGAGCATTGGTTGCATCTTTGCAGAGATGGTGACTCGAAAAGCCCTGTTTCCTGGTGACTCTGAGATTGACCAGCTCTTTCGTATCTTTCGTATGCTGGGGACACCCAGCGAAGACACATGGCCCGGGGTCACCCAGCTGCCTGACTATAAGGGCAGCTTCCCTAAGTGGACCAGGAAGGGACTGGAAGAGATTGTGCCCAATCTGGAGCCAGAGGGCAGGGACCTGCTCATGCAACTCCTGCAGTATGACCCCAGCCAGCGGATCACAGCCAAGACTGCCCTGGCCCACCCGTACTTCTCATCCCCTGAGCCCTCCCCAGCTGCCCGCCAGTATGTGCTGCAGCGATTCCGCCATTGAGAATGTCAAGGCCACACTCAGATCCTTTCTCGAGCAGCAGCTGCTGCCCCAGCTGCCTCCTACCCATTGCCAAGAGAGGATGCATCTGGGGAGAGCAAAGCACTAAGGAATTCAGCATCAGCCTGCAGAGGGCTGAGTCTGGGTTAGTCCTGCCC
5612
PRIMER_LEFT_EXPLAIN=considered 9505, would not amplify any of the ORF 6576, GC content failed 22, low tm 213, high tm 1911, high end compl 5,high 3' stability 39, ok 739
5613
PRIMER_RIGHT_EXPLAIN=considered 9326, GC content failed 54, low tm 682, high tm 6041, high any compl 1, high end compl 3,high 3' stability 58, ok 2487
5614
PRIMER_PAIR_EXPLAIN=considered 49, ok 49
5615
PRIMER_STOP_CODON_POSITION=1003
5616
PRIMER_PAIR_PENALTY=9.6633
5617
PRIMER_LEFT_PENALTY=5.563683
5618
PRIMER_RIGHT_PENALTY=4.099578
5619
PRIMER_LEFT_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA
5620
PRIMER_RIGHT_SEQUENCE=ACATTCTCAATGGCGGAATC
5621
PRIMER_LEFT=88,25
5622
PRIMER_RIGHT=1011,20
5623
PRIMER_LEFT_TM=59.436
5624
PRIMER_RIGHT_TM=59.900
5625
PRIMER_LEFT_GC_PERCENT=40.000
5626
PRIMER_RIGHT_GC_PERCENT=45.000
5627
PRIMER_LEFT_SELF_ANY=4.00
5628
PRIMER_RIGHT_SELF_ANY=5.00
5629
PRIMER_LEFT_SELF_END=0.00
5630
PRIMER_RIGHT_SELF_END=3.00
5631
PRIMER_LEFT_POSITION_PENALTY=0.000000
5632
PRIMER_RIGHT_POSITION_PENALTY=4.000000
5633
PRIMER_LEFT_END_STABILITY=6.4000
5634
PRIMER_RIGHT_END_STABILITY=6.6000
5635
PRIMER_PAIR_COMPL_ANY=5.00
5636
PRIMER_PAIR_COMPL_END=2.00
5637
PRIMER_PRODUCT_SIZE=924
5638
PRIMER_PAIR_PENALTY_1=10.3311
5639
PRIMER_LEFT_1_PENALTY=6.231558
5640
PRIMER_RIGHT_1_PENALTY=4.099578
5641
PRIMER_LEFT_1_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAG
5642
PRIMER_RIGHT_1_SEQUENCE=ACATTCTCAATGGCGGAATC
5643
PRIMER_LEFT_1=88,24
5644
PRIMER_RIGHT_1=1011,20
5645
PRIMER_LEFT_1_TM=57.768
5646
PRIMER_RIGHT_1_TM=59.900
5647
PRIMER_LEFT_1_GC_PERCENT=41.667
5648
PRIMER_RIGHT_1_GC_PERCENT=45.000
5649
PRIMER_LEFT_1_SELF_ANY=4.00
5650
PRIMER_RIGHT_1_SELF_ANY=5.00
5651
PRIMER_LEFT_1_SELF_END=0.00
5652
PRIMER_RIGHT_1_SELF_END=3.00
5653
PRIMER_LEFT_1_POSITION_PENALTY=0.000000
5654
PRIMER_RIGHT_1_POSITION_PENALTY=4.000000
5655
PRIMER_LEFT_1_END_STABILITY=5.7000
5656
PRIMER_RIGHT_1_END_STABILITY=6.6000
5657
PRIMER_PAIR_1_COMPL_ANY=5.00
5658
PRIMER_PAIR_1_COMPL_END=1.00
5659
PRIMER_PRODUCT_SIZE_1=924
5660
PRIMER_PAIR_PENALTY_2=10.5213
5661
PRIMER_LEFT_2_PENALTY=5.563683
5662
PRIMER_RIGHT_2_PENALTY=4.957587
5663
PRIMER_LEFT_2_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA
5664
PRIMER_RIGHT_2_SEQUENCE=ATTCTCAATGGCGGAATCG
5665
PRIMER_LEFT_2=88,25
5666
PRIMER_RIGHT_2=1009,19
5667
PRIMER_LEFT_2_TM=59.436
5668
PRIMER_RIGHT_2_TM=60.958
5669
PRIMER_LEFT_2_GC_PERCENT=40.000
5670
PRIMER_RIGHT_2_GC_PERCENT=47.368
5671
PRIMER_LEFT_2_SELF_ANY=4.00
5672
PRIMER_RIGHT_2_SELF_ANY=4.00
5673
PRIMER_LEFT_2_SELF_END=0.00
5674
PRIMER_RIGHT_2_SELF_END=2.00
5675
PRIMER_LEFT_2_POSITION_PENALTY=0.000000
5676
PRIMER_RIGHT_2_POSITION_PENALTY=3.000000
5677
PRIMER_LEFT_2_END_STABILITY=6.4000
5678
PRIMER_RIGHT_2_END_STABILITY=8.6000
5679
PRIMER_PAIR_2_COMPL_ANY=4.00
5680
PRIMER_PAIR_2_COMPL_END=1.00
5681
PRIMER_PRODUCT_SIZE_2=922
5682
PRIMER_PAIR_PENALTY_3=10.6709
5683
PRIMER_LEFT_3_PENALTY=6.571364
5684
PRIMER_RIGHT_3_PENALTY=4.099578
5685
PRIMER_LEFT_3_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGAA
5686
PRIMER_RIGHT_3_SEQUENCE=ACATTCTCAATGGCGGAATC
5687
PRIMER_LEFT_3=88,26
5688
PRIMER_RIGHT_3=1011,20
5689
PRIMER_LEFT_3_TM=60.571
5690
PRIMER_RIGHT_3_TM=59.900
5691
PRIMER_LEFT_3_GC_PERCENT=38.462
5692
PRIMER_RIGHT_3_GC_PERCENT=45.000
5693
PRIMER_LEFT_3_SELF_ANY=4.00
5694
PRIMER_RIGHT_3_SELF_ANY=5.00
5695
PRIMER_LEFT_3_SELF_END=0.00
5696
PRIMER_RIGHT_3_SELF_END=3.00
5697
PRIMER_LEFT_3_POSITION_PENALTY=0.000000
5698
PRIMER_RIGHT_3_POSITION_PENALTY=4.000000
5699
PRIMER_LEFT_3_END_STABILITY=6.7000
5700
PRIMER_RIGHT_3_END_STABILITY=6.6000
5701
PRIMER_PAIR_3_COMPL_ANY=5.00
5702
PRIMER_PAIR_3_COMPL_END=2.00
5703
PRIMER_PRODUCT_SIZE_3=924
5704
PRIMER_PAIR_PENALTY_4=11.0485
5705
PRIMER_LEFT_4_PENALTY=5.563683
5706
PRIMER_RIGHT_4_PENALTY=5.484828
5707
PRIMER_LEFT_4_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA
5708
PRIMER_RIGHT_4_SEQUENCE=CATTCTCAATGGCGGAATC
5709
PRIMER_LEFT_4=88,25
5710
PRIMER_RIGHT_4=1010,19
5711
PRIMER_LEFT_4_TM=59.436
5712
PRIMER_RIGHT_4_TM=59.015
5713
PRIMER_LEFT_4_GC_PERCENT=40.000
5714
PRIMER_RIGHT_4_GC_PERCENT=47.368
5715
PRIMER_LEFT_4_SELF_ANY=4.00
5716
PRIMER_RIGHT_4_SELF_ANY=5.00
5717
PRIMER_LEFT_4_SELF_END=0.00
5718
PRIMER_RIGHT_4_SELF_END=3.00
5719
PRIMER_LEFT_4_POSITION_PENALTY=0.000000
5720
PRIMER_RIGHT_4_POSITION_PENALTY=3.500000
5721
PRIMER_LEFT_4_END_STABILITY=6.4000
5722
PRIMER_RIGHT_4_END_STABILITY=6.6000
5723
PRIMER_PAIR_4_COMPL_ANY=4.00
5724
PRIMER_PAIR_4_COMPL_END=2.00
5725
PRIMER_PRODUCT_SIZE_4=923
5726
PRIMER_PAIR_PENALTY_5=11.1891
5727
PRIMER_LEFT_5_PENALTY=6.231558
5728
PRIMER_RIGHT_5_PENALTY=4.957587
5729
PRIMER_LEFT_5_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAG
5730
PRIMER_RIGHT_5_SEQUENCE=ATTCTCAATGGCGGAATCG
5731
PRIMER_LEFT_5=88,24
5732
PRIMER_RIGHT_5=1009,19
5733
PRIMER_LEFT_5_TM=57.768
5734
PRIMER_RIGHT_5_TM=60.958
5735
PRIMER_LEFT_5_GC_PERCENT=41.667
5736
PRIMER_RIGHT_5_GC_PERCENT=47.368
5737
PRIMER_LEFT_5_SELF_ANY=4.00
5738
PRIMER_RIGHT_5_SELF_ANY=4.00
5739
PRIMER_LEFT_5_SELF_END=0.00
5740
PRIMER_RIGHT_5_SELF_END=2.00
5741
PRIMER_LEFT_5_POSITION_PENALTY=0.000000
5742
PRIMER_RIGHT_5_POSITION_PENALTY=3.000000
5743
PRIMER_LEFT_5_END_STABILITY=5.7000
5744
PRIMER_RIGHT_5_END_STABILITY=8.6000
5745
PRIMER_PAIR_5_COMPL_ANY=4.00
5746
PRIMER_PAIR_5_COMPL_END=0.00
5747
PRIMER_PRODUCT_SIZE_5=922
5748
PRIMER_PAIR_PENALTY_6=11.5290
5749
PRIMER_LEFT_6_PENALTY=6.571364
5750
PRIMER_RIGHT_6_PENALTY=4.957587
5751
PRIMER_LEFT_6_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGAA
5752
PRIMER_RIGHT_6_SEQUENCE=ATTCTCAATGGCGGAATCG
5753
PRIMER_LEFT_6=88,26
5754
PRIMER_RIGHT_6=1009,19
5755
PRIMER_LEFT_6_TM=60.571
5756
PRIMER_RIGHT_6_TM=60.958
5757
PRIMER_LEFT_6_GC_PERCENT=38.462
5758
PRIMER_RIGHT_6_GC_PERCENT=47.368
5759
PRIMER_LEFT_6_SELF_ANY=4.00
5760
PRIMER_RIGHT_6_SELF_ANY=4.00
5761
PRIMER_LEFT_6_SELF_END=0.00
5762
PRIMER_RIGHT_6_SELF_END=2.00
5763
PRIMER_LEFT_6_POSITION_PENALTY=0.000000
5764
PRIMER_RIGHT_6_POSITION_PENALTY=3.000000
5765
PRIMER_LEFT_6_END_STABILITY=6.7000
5766
PRIMER_RIGHT_6_END_STABILITY=8.6000
5767
PRIMER_PAIR_6_COMPL_ANY=5.00
5768
PRIMER_PAIR_6_COMPL_END=1.00
5769
PRIMER_PRODUCT_SIZE_6=922
5770
PRIMER_PAIR_PENALTY_7=11.7164
5771
PRIMER_LEFT_7_PENALTY=6.231558
5772
PRIMER_RIGHT_7_PENALTY=5.484828
5773
PRIMER_LEFT_7_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAG
5774
PRIMER_RIGHT_7_SEQUENCE=CATTCTCAATGGCGGAATC
5775
PRIMER_LEFT_7=88,24
5776
PRIMER_RIGHT_7=1010,19
5777
PRIMER_LEFT_7_TM=57.768
5778
PRIMER_RIGHT_7_TM=59.015
5779
PRIMER_LEFT_7_GC_PERCENT=41.667
5780
PRIMER_RIGHT_7_GC_PERCENT=47.368
5781
PRIMER_LEFT_7_SELF_ANY=4.00
5782
PRIMER_RIGHT_7_SELF_ANY=5.00
5783
PRIMER_LEFT_7_SELF_END=0.00
5784
PRIMER_RIGHT_7_SELF_END=3.00
5785
PRIMER_LEFT_7_POSITION_PENALTY=0.000000
5786
PRIMER_RIGHT_7_POSITION_PENALTY=3.500000
5787
PRIMER_LEFT_7_END_STABILITY=5.7000
5788
PRIMER_RIGHT_7_END_STABILITY=6.6000
5789
PRIMER_PAIR_7_COMPL_ANY=4.00
5790
PRIMER_PAIR_7_COMPL_END=1.00
5791
PRIMER_PRODUCT_SIZE_7=923
5792
PRIMER_PAIR_PENALTY_8=12.0562
5793
PRIMER_LEFT_8_PENALTY=6.571364
5794
PRIMER_RIGHT_8_PENALTY=5.484828
5795
PRIMER_LEFT_8_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGAA
5796
PRIMER_RIGHT_8_SEQUENCE=CATTCTCAATGGCGGAATC
5797
PRIMER_LEFT_8=88,26
5798
PRIMER_RIGHT_8=1010,19
5799
PRIMER_LEFT_8_TM=60.571
5800
PRIMER_RIGHT_8_TM=59.015
5801
PRIMER_LEFT_8_GC_PERCENT=38.462
5802
PRIMER_RIGHT_8_GC_PERCENT=47.368
5803
PRIMER_LEFT_8_SELF_ANY=4.00
5804
PRIMER_RIGHT_8_SELF_ANY=5.00
5805
PRIMER_LEFT_8_SELF_END=0.00
5806
PRIMER_RIGHT_8_SELF_END=3.00
5807
PRIMER_LEFT_8_POSITION_PENALTY=0.000000
5808
PRIMER_RIGHT_8_POSITION_PENALTY=3.500000
5809
PRIMER_LEFT_8_END_STABILITY=6.7000
5810
PRIMER_RIGHT_8_END_STABILITY=6.6000
5811
PRIMER_PAIR_8_COMPL_ANY=5.00
5812
PRIMER_PAIR_8_COMPL_END=2.00
5813
PRIMER_PRODUCT_SIZE_8=923
5814
PRIMER_PAIR_PENALTY_9=12.4165
5815
PRIMER_LEFT_9_PENALTY=8.316960
5816
PRIMER_RIGHT_9_PENALTY=4.099578
5817
PRIMER_LEFT_9_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGAAG
5818
PRIMER_RIGHT_9_SEQUENCE=ACATTCTCAATGGCGGAATC
5819
PRIMER_LEFT_9=88,27
5820
PRIMER_RIGHT_9=1011,20
5821
PRIMER_LEFT_9_TM=61.317
5822
PRIMER_RIGHT_9_TM=59.900
5823
PRIMER_LEFT_9_GC_PERCENT=40.741
5824
PRIMER_RIGHT_9_GC_PERCENT=45.000
5825
PRIMER_LEFT_9_SELF_ANY=4.00
5826
PRIMER_RIGHT_9_SELF_ANY=5.00
5827
PRIMER_LEFT_9_SELF_END=0.00
5828
PRIMER_RIGHT_9_SELF_END=3.00
5829
PRIMER_LEFT_9_POSITION_PENALTY=0.000000
5830
PRIMER_RIGHT_9_POSITION_PENALTY=4.000000
5831
PRIMER_LEFT_9_END_STABILITY=6.7000
5832
PRIMER_RIGHT_9_END_STABILITY=6.6000
5833
PRIMER_PAIR_9_COMPL_ANY=5.00
5834
PRIMER_PAIR_9_COMPL_END=1.00
5835
PRIMER_PRODUCT_SIZE_9=924
5836
PRIMER_PAIR_PENALTY_10=12.6695
5837
PRIMER_LEFT_10_PENALTY=5.563683
5838
PRIMER_RIGHT_10_PENALTY=7.105834
5839
PRIMER_LEFT_10_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA
5840
PRIMER_RIGHT_10_SEQUENCE=TCTCAATGGCGGAATCGCT
5841
PRIMER_LEFT_10=88,25
5842
PRIMER_RIGHT_10=1007,19
5843
PRIMER_LEFT_10_TM=59.436
5844
PRIMER_RIGHT_10_TM=64.106
5845
PRIMER_LEFT_10_GC_PERCENT=40.000
5846
PRIMER_RIGHT_10_GC_PERCENT=52.632
5847
PRIMER_LEFT_10_SELF_ANY=4.00
5848
PRIMER_RIGHT_10_SELF_ANY=3.00
5849
PRIMER_LEFT_10_SELF_END=0.00
5850
PRIMER_RIGHT_10_SELF_END=2.00
5851
PRIMER_LEFT_10_POSITION_PENALTY=0.000000
5852
PRIMER_RIGHT_10_POSITION_PENALTY=2.000000
5853
PRIMER_LEFT_10_END_STABILITY=6.4000
5854
PRIMER_RIGHT_10_END_STABILITY=9.9000
5855
PRIMER_PAIR_10_COMPL_ANY=4.00
5856
PRIMER_PAIR_10_COMPL_END=3.00
5857
PRIMER_PRODUCT_SIZE_10=920
5858
PRIMER_PAIR_PENALTY_11=12.7766
5859
PRIMER_LEFT_11_PENALTY=5.563683
5860
PRIMER_RIGHT_11_PENALTY=7.212929
5861
PRIMER_LEFT_11_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA
5862
PRIMER_RIGHT_11_SEQUENCE=CATTCTCAATGGCGGAATCG
5863
PRIMER_LEFT_11=88,25
5864
PRIMER_RIGHT_11=1010,20
5865
PRIMER_LEFT_11_TM=59.436
5866
PRIMER_RIGHT_11_TM=63.713
5867
PRIMER_LEFT_11_GC_PERCENT=40.000
5868
PRIMER_RIGHT_11_GC_PERCENT=50.000
5869
PRIMER_LEFT_11_SELF_ANY=4.00
5870
PRIMER_RIGHT_11_SELF_ANY=5.00
5871
PRIMER_LEFT_11_SELF_END=0.00
5872
PRIMER_RIGHT_11_SELF_END=2.00
5873
PRIMER_LEFT_11_POSITION_PENALTY=0.000000
5874
PRIMER_RIGHT_11_POSITION_PENALTY=3.500000
5875
PRIMER_LEFT_11_END_STABILITY=6.4000
5876
PRIMER_RIGHT_11_END_STABILITY=8.6000
5877
PRIMER_PAIR_11_COMPL_ANY=4.00
5878
PRIMER_PAIR_11_COMPL_END=1.00
5879
PRIMER_PRODUCT_SIZE_11=923
5880
PRIMER_PAIR_PENALTY_12=12.7991
5881
PRIMER_LEFT_12_PENALTY=5.563683
5882
PRIMER_RIGHT_12_PENALTY=7.235377
5883
PRIMER_LEFT_12_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA
5884
PRIMER_RIGHT_12_SEQUENCE=GACATTCTCAATGGCGGAATC
5885
PRIMER_LEFT_12=88,25
5886
PRIMER_RIGHT_12=1012,21
5887
PRIMER_LEFT_12_TM=59.436
5888
PRIMER_RIGHT_12_TM=61.735
5889
PRIMER_LEFT_12_GC_PERCENT=40.000
5890
PRIMER_RIGHT_12_GC_PERCENT=47.619
5891
PRIMER_LEFT_12_SELF_ANY=4.00
5892
PRIMER_RIGHT_12_SELF_ANY=5.00
5893
PRIMER_LEFT_12_SELF_END=0.00
5894
PRIMER_RIGHT_12_SELF_END=3.00
5895
PRIMER_LEFT_12_POSITION_PENALTY=0.000000
5896
PRIMER_RIGHT_12_POSITION_PENALTY=4.500000
5897
PRIMER_LEFT_12_END_STABILITY=6.4000
5898
PRIMER_RIGHT_12_END_STABILITY=6.6000
5899
PRIMER_PAIR_12_COMPL_ANY=5.00
5900
PRIMER_PAIR_12_COMPL_END=2.00
5901
PRIMER_PRODUCT_SIZE_12=925
5902
PRIMER_PAIR_PENALTY_13=13.0556
5903
PRIMER_LEFT_13_PENALTY=5.563683
5904
PRIMER_RIGHT_13_PENALTY=7.491945
5905
PRIMER_LEFT_13_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA
5906
PRIMER_RIGHT_13_SEQUENCE=ATTCTCAATGGCGGAATCGC
5907
PRIMER_LEFT_13=88,25
5908
PRIMER_RIGHT_13=1009,20
5909
PRIMER_LEFT_13_TM=59.436
5910
PRIMER_RIGHT_13_TM=64.492
5911
PRIMER_LEFT_13_GC_PERCENT=40.000
5912
PRIMER_RIGHT_13_GC_PERCENT=50.000
5913
PRIMER_LEFT_13_SELF_ANY=4.00
5914
PRIMER_RIGHT_13_SELF_ANY=4.00
5915
PRIMER_LEFT_13_SELF_END=0.00
5916
PRIMER_RIGHT_13_SELF_END=3.00
5917
PRIMER_LEFT_13_POSITION_PENALTY=0.000000
5918
PRIMER_RIGHT_13_POSITION_PENALTY=3.000000
5919
PRIMER_LEFT_13_END_STABILITY=6.4000
5920
PRIMER_RIGHT_13_END_STABILITY=9.8000
5921
PRIMER_PAIR_13_COMPL_ANY=4.00
5922
PRIMER_PAIR_13_COMPL_END=2.00
5923
PRIMER_PRODUCT_SIZE_13=922
5924
PRIMER_PAIR_PENALTY_14=13.2745
5925
PRIMER_LEFT_14_PENALTY=8.316960
5926
PRIMER_RIGHT_14_PENALTY=4.957587
5927
PRIMER_LEFT_14_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGAAG
5928
PRIMER_RIGHT_14_SEQUENCE=ATTCTCAATGGCGGAATCG
5929
PRIMER_LEFT_14=88,27
5930
PRIMER_RIGHT_14=1009,19
5931
PRIMER_LEFT_14_TM=61.317
5932
PRIMER_RIGHT_14_TM=60.958
5933
PRIMER_LEFT_14_GC_PERCENT=40.741
5934
PRIMER_RIGHT_14_GC_PERCENT=47.368
5935
PRIMER_LEFT_14_SELF_ANY=4.00
5936
PRIMER_RIGHT_14_SELF_ANY=4.00
5937
PRIMER_LEFT_14_SELF_END=0.00
5938
PRIMER_RIGHT_14_SELF_END=2.00
5939
PRIMER_LEFT_14_POSITION_PENALTY=0.000000
5940
PRIMER_RIGHT_14_POSITION_PENALTY=3.000000
5941
PRIMER_LEFT_14_END_STABILITY=6.7000
5942
PRIMER_RIGHT_14_END_STABILITY=8.6000
5943
PRIMER_PAIR_14_COMPL_ANY=5.00
5944
PRIMER_PAIR_14_COMPL_END=0.00
5945
PRIMER_PRODUCT_SIZE_14=922
5946
PRIMER_PAIR_PENALTY_15=13.3374
5947
PRIMER_LEFT_15_PENALTY=6.231558
5948
PRIMER_RIGHT_15_PENALTY=7.105834
5949
PRIMER_LEFT_15_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAG
5950
PRIMER_RIGHT_15_SEQUENCE=TCTCAATGGCGGAATCGCT
5951
PRIMER_LEFT_15=88,24
5952
PRIMER_RIGHT_15=1007,19
5953
PRIMER_LEFT_15_TM=57.768
5954
PRIMER_RIGHT_15_TM=64.106
5955
PRIMER_LEFT_15_GC_PERCENT=41.667
5956
PRIMER_RIGHT_15_GC_PERCENT=52.632
5957
PRIMER_LEFT_15_SELF_ANY=4.00
5958
PRIMER_RIGHT_15_SELF_ANY=3.00
5959
PRIMER_LEFT_15_SELF_END=0.00
5960
PRIMER_RIGHT_15_SELF_END=2.00
5961
PRIMER_LEFT_15_POSITION_PENALTY=0.000000
5962
PRIMER_RIGHT_15_POSITION_PENALTY=2.000000
5963
PRIMER_LEFT_15_END_STABILITY=5.7000
5964
PRIMER_RIGHT_15_END_STABILITY=9.9000
5965
PRIMER_PAIR_15_COMPL_ANY=4.00
5966
PRIMER_PAIR_15_COMPL_END=2.00
5967
PRIMER_PRODUCT_SIZE_15=920
5968
PRIMER_PAIR_PENALTY_16=13.4445
5969
PRIMER_LEFT_16_PENALTY=6.231558
5970
PRIMER_RIGHT_16_PENALTY=7.212929
5971
PRIMER_LEFT_16_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAG
5972
PRIMER_RIGHT_16_SEQUENCE=CATTCTCAATGGCGGAATCG
5973
PRIMER_LEFT_16=88,24
5974
PRIMER_RIGHT_16=1010,20
5975
PRIMER_LEFT_16_TM=57.768
5976
PRIMER_RIGHT_16_TM=63.713
5977
PRIMER_LEFT_16_GC_PERCENT=41.667
5978
PRIMER_RIGHT_16_GC_PERCENT=50.000
5979
PRIMER_LEFT_16_SELF_ANY=4.00
5980
PRIMER_RIGHT_16_SELF_ANY=5.00
5981
PRIMER_LEFT_16_SELF_END=0.00
5982
PRIMER_RIGHT_16_SELF_END=2.00
5983
PRIMER_LEFT_16_POSITION_PENALTY=0.000000
5984
PRIMER_RIGHT_16_POSITION_PENALTY=3.500000
5985
PRIMER_LEFT_16_END_STABILITY=5.7000
5986
PRIMER_RIGHT_16_END_STABILITY=8.6000
5987
PRIMER_PAIR_16_COMPL_ANY=4.00
5988
PRIMER_PAIR_16_COMPL_END=0.00
5989
PRIMER_PRODUCT_SIZE_16=923
5990
PRIMER_PAIR_PENALTY_17=13.4669
5991
PRIMER_LEFT_17_PENALTY=6.231558
5992
PRIMER_RIGHT_17_PENALTY=7.235377
5993
PRIMER_LEFT_17_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAG
5994
PRIMER_RIGHT_17_SEQUENCE=GACATTCTCAATGGCGGAATC
5995
PRIMER_LEFT_17=88,24
5996
PRIMER_RIGHT_17=1012,21
5997
PRIMER_LEFT_17_TM=57.768
5998
PRIMER_RIGHT_17_TM=61.735
5999
PRIMER_LEFT_17_GC_PERCENT=41.667
6000
PRIMER_RIGHT_17_GC_PERCENT=47.619
6001
PRIMER_LEFT_17_SELF_ANY=4.00
6002
PRIMER_RIGHT_17_SELF_ANY=5.00
6003
PRIMER_LEFT_17_SELF_END=0.00
6004
PRIMER_RIGHT_17_SELF_END=3.00
6005
PRIMER_LEFT_17_POSITION_PENALTY=0.000000
6006
PRIMER_RIGHT_17_POSITION_PENALTY=4.500000
6007
PRIMER_LEFT_17_END_STABILITY=5.7000
6008
PRIMER_RIGHT_17_END_STABILITY=6.6000
6009
PRIMER_PAIR_17_COMPL_ANY=5.00
6010
PRIMER_PAIR_17_COMPL_END=1.00
6011
PRIMER_PRODUCT_SIZE_17=925
6012
PRIMER_PAIR_PENALTY_18=13.5226
6013
PRIMER_LEFT_18_PENALTY=5.563683
6014
PRIMER_RIGHT_18_PENALTY=7.958919
6015
PRIMER_LEFT_18_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA
6016
PRIMER_RIGHT_18_SEQUENCE=TTCTCAATGGCGGAATCGC
6017
PRIMER_LEFT_18=88,25
6018
PRIMER_RIGHT_18=1008,19
6019
PRIMER_LEFT_18_TM=59.436
6020
PRIMER_RIGHT_18_TM=64.459
6021
PRIMER_LEFT_18_GC_PERCENT=40.000
6022
PRIMER_RIGHT_18_GC_PERCENT=52.632
6023
PRIMER_LEFT_18_SELF_ANY=4.00
6024
PRIMER_RIGHT_18_SELF_ANY=3.00
6025
PRIMER_LEFT_18_SELF_END=0.00
6026
PRIMER_RIGHT_18_SELF_END=3.00
6027
PRIMER_LEFT_18_POSITION_PENALTY=0.000000
6028
PRIMER_RIGHT_18_POSITION_PENALTY=2.500000
6029
PRIMER_LEFT_18_END_STABILITY=6.4000
6030
PRIMER_RIGHT_18_END_STABILITY=9.8000
6031
PRIMER_PAIR_18_COMPL_ANY=4.00
6032
PRIMER_PAIR_18_COMPL_END=2.00
6033
PRIMER_PRODUCT_SIZE_18=921
6034
PRIMER_PAIR_PENALTY_19=13.6772
6035
PRIMER_LEFT_19_PENALTY=6.571364
6036
PRIMER_RIGHT_19_PENALTY=7.105834
6037
PRIMER_LEFT_19_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGAA
6038
PRIMER_RIGHT_19_SEQUENCE=TCTCAATGGCGGAATCGCT
6039
PRIMER_LEFT_19=88,26
6040
PRIMER_RIGHT_19=1007,19
6041
PRIMER_LEFT_19_TM=60.571
6042
PRIMER_RIGHT_19_TM=64.106
6043
PRIMER_LEFT_19_GC_PERCENT=38.462
6044
PRIMER_RIGHT_19_GC_PERCENT=52.632
6045
PRIMER_LEFT_19_SELF_ANY=4.00
6046
PRIMER_RIGHT_19_SELF_ANY=3.00
6047
PRIMER_LEFT_19_SELF_END=0.00
6048
PRIMER_RIGHT_19_SELF_END=2.00
6049
PRIMER_LEFT_19_POSITION_PENALTY=0.000000
6050
PRIMER_RIGHT_19_POSITION_PENALTY=2.000000
6051
PRIMER_LEFT_19_END_STABILITY=6.7000
6052
PRIMER_RIGHT_19_END_STABILITY=9.9000
6053
PRIMER_PAIR_19_COMPL_ANY=4.00
6054
PRIMER_PAIR_19_COMPL_END=2.00
6055
PRIMER_PRODUCT_SIZE_19=920
6056
=
6057
PRIMER_SEQUENCE_ID=X66364/CDK5
6058
PRIMER_COMMENT=H.sapiens mRNA PSSALRE for serine/threonine protein kinase. CDS=25..903
6059
PRIMER_START_CODON_POSITION=24
6060
SEQUENCE=CGCAGGGGTCCCCCGGCCGCCGCGATGCAGAAATACGAGAAACTGGAAAAGATTGGGGAAGGCACCTACGGAACTGTGTTCAAGGCCAAAAACCGGGAGACTCATGAGATCGTGGCTCTGAAACGGGTGAGGCTGGATGACGATGATGAGGGTGTGCCGAGTTCCGCCCTCCGGGAGATCTGCCTACTCAAGGAGCTGAAGCACAAGAACATCGTCAGGCTTCATGACGTCCTGCACAGCGACAAGAAGCTGACTTTGGTTTTTGAATTCTGTGACCAGGACCTGAAGAAGTATTTTGACAGTTGCAATGGTGACCTCGATCCTGAGATTGTAAAGTCATTCCTCTTCCAGCTACTAAAAGGGCTGGGATTCTGTCATAGCCGCAATGTGCTACACAGGGACCTGAAGCCCCAGAACCTGCTAATAAACAGGAATGGGGAGCTGAAATTGGCTGATTTTGGCCTGGCTCGAGCCTTTGGGATTCCCGTCCGCTGTTACTCAGCTGAGGTGGTCACACTGTGGTACCGCCCACCGGATGTCCTCTTTGGGGCCAAGCTGTACTCCACGTCCATCGACATGTGGTCAGCCGGCTGCATCTTTGCAGAGCTGGCCAATGCTGGGCGGCCTCTTTTTCCCGGCAATGATGTCGATGACCAGTTGAAGAGGATCTTCCGACTGCTGGGGACGCCCACCGAGGAGCAGTGGCCCTCTATGACCAAGCTGCCAGACTATAAGCCCTATCCGATGTACCCGGCCACAACATCCCTGGTGAACGTCGTGCCCAAACTCAATGCCACAGGGAGGGATCTGCTGCAGAACCTTCTGAAGTGTAACCCTGTCCAGCGTATCTCAGCAGAAGAGGCCCTGCAGCACCCCTACTTCTCCGACTTCTGTCCGCCCTAGGCCCCGGGACCCCCGCCTCCAGGCTGGGCCTGGCCTATTTAAGCCCCCTCTTGAGAGGGTGAGACAGTGGGGGTGCCTGG
6061
PRIMER_LEFT_EXPLAIN=considered 7904, would not amplify any of the ORF 5278, GC content failed 48, low tm 249, high tm 1503,high 3' stability 40, ok 786
6062
PRIMER_RIGHT_EXPLAIN=considered 7709, GC content failed 194, low tm 667, high tm 4515,high 3' stability 60, ok 2273
6063
PRIMER_PAIR_EXPLAIN=considered 48, ok 48
6064
PRIMER_STOP_CODON_POSITION=900
6065
PRIMER_PAIR_PENALTY=5.8309
6066
PRIMER_LEFT_PENALTY=3.142611
6067
PRIMER_RIGHT_PENALTY=2.688260
6068
PRIMER_LEFT_SEQUENCE=ATGCAGAAATACGAGAAACTGGA
6069
PRIMER_RIGHT_SEQUENCE=CCTAGGGCGGACAGAAGTC
6070
PRIMER_LEFT=24,23
6071
PRIMER_RIGHT=903,19
6072
PRIMER_LEFT_TM=60.143
6073
PRIMER_RIGHT_TM=59.812
6074
PRIMER_LEFT_GC_PERCENT=39.130
6075
PRIMER_RIGHT_GC_PERCENT=63.158
6076
PRIMER_LEFT_SELF_ANY=4.00
6077
PRIMER_RIGHT_SELF_ANY=6.00
6078
PRIMER_LEFT_SELF_END=0.00
6079
PRIMER_RIGHT_SELF_END=2.00
6080
PRIMER_LEFT_POSITION_PENALTY=0.000000
6081
PRIMER_RIGHT_POSITION_PENALTY=1.500000
6082
PRIMER_LEFT_END_STABILITY=8.2000
6083
PRIMER_RIGHT_END_STABILITY=6.4000
6084
PRIMER_PAIR_COMPL_ANY=3.00
6085
PRIMER_PAIR_COMPL_END=2.00
6086
PRIMER_PRODUCT_SIZE=880
6087
PRIMER_PAIR_PENALTY_1=6.0811
6088
PRIMER_LEFT_1_PENALTY=3.142611
6089
PRIMER_RIGHT_1_PENALTY=2.938464
6090
PRIMER_LEFT_1_SEQUENCE=ATGCAGAAATACGAGAAACTGGA
6091
PRIMER_RIGHT_1_SEQUENCE=CTAGGGCGGACAGAAGTCG
6092
PRIMER_LEFT_1=24,23
6093
PRIMER_RIGHT_1=902,19
6094
PRIMER_LEFT_1_TM=60.143
6095
PRIMER_RIGHT_1_TM=60.938
6096
PRIMER_LEFT_1_GC_PERCENT=39.130
6097
PRIMER_RIGHT_1_GC_PERCENT=63.158
6098
PRIMER_LEFT_1_SELF_ANY=4.00
6099
PRIMER_RIGHT_1_SELF_ANY=4.00
6100
PRIMER_LEFT_1_SELF_END=0.00
6101
PRIMER_RIGHT_1_SELF_END=2.00
6102
PRIMER_LEFT_1_POSITION_PENALTY=0.000000
6103
PRIMER_RIGHT_1_POSITION_PENALTY=1.000000
6104
PRIMER_LEFT_1_END_STABILITY=8.2000
6105
PRIMER_RIGHT_1_END_STABILITY=8.1000
6106
PRIMER_PAIR_1_COMPL_ANY=3.00
6107
PRIMER_PAIR_1_COMPL_END=1.00
6108
PRIMER_PRODUCT_SIZE_1=879
6109
PRIMER_PAIR_PENALTY_2=6.2856
6110
PRIMER_LEFT_2_PENALTY=3.597306
6111
PRIMER_RIGHT_2_PENALTY=2.688260
6112
PRIMER_LEFT_2_SEQUENCE=ATGCAGAAATACGAGAAACTGG
6113
PRIMER_RIGHT_2_SEQUENCE=CCTAGGGCGGACAGAAGTC
6114
PRIMER_LEFT_2=24,22
6115
PRIMER_RIGHT_2=903,19
6116
PRIMER_LEFT_2_TM=58.403
6117
PRIMER_RIGHT_2_TM=59.812
6118
PRIMER_LEFT_2_GC_PERCENT=40.909
6119
PRIMER_RIGHT_2_GC_PERCENT=63.158
6120
PRIMER_LEFT_2_SELF_ANY=4.00
6121
PRIMER_RIGHT_2_SELF_ANY=6.00
6122
PRIMER_LEFT_2_SELF_END=1.00
6123
PRIMER_RIGHT_2_SELF_END=2.00
6124
PRIMER_LEFT_2_POSITION_PENALTY=0.000000
6125
PRIMER_RIGHT_2_POSITION_PENALTY=1.500000
6126
PRIMER_LEFT_2_END_STABILITY=7.9000
6127
PRIMER_RIGHT_2_END_STABILITY=6.4000
6128
PRIMER_PAIR_2_COMPL_ANY=3.00
6129
PRIMER_PAIR_2_COMPL_END=1.00
6130
PRIMER_PRODUCT_SIZE_2=880
6131
PRIMER_PAIR_PENALTY_3=6.5358
6132
PRIMER_LEFT_3_PENALTY=3.597306
6133
PRIMER_RIGHT_3_PENALTY=2.938464
6134
PRIMER_LEFT_3_SEQUENCE=ATGCAGAAATACGAGAAACTGG
6135
PRIMER_RIGHT_3_SEQUENCE=CTAGGGCGGACAGAAGTCG
6136
PRIMER_LEFT_3=24,22
6137
PRIMER_RIGHT_3=902,19
6138
PRIMER_LEFT_3_TM=58.403
6139
PRIMER_RIGHT_3_TM=60.938
6140
PRIMER_LEFT_3_GC_PERCENT=40.909
6141
PRIMER_RIGHT_3_GC_PERCENT=63.158
6142
PRIMER_LEFT_3_SELF_ANY=4.00
6143
PRIMER_RIGHT_3_SELF_ANY=4.00
6144
PRIMER_LEFT_3_SELF_END=1.00
6145
PRIMER_RIGHT_3_SELF_END=2.00
6146
PRIMER_LEFT_3_POSITION_PENALTY=0.000000
6147
PRIMER_RIGHT_3_POSITION_PENALTY=1.000000
6148
PRIMER_LEFT_3_END_STABILITY=7.9000
6149
PRIMER_RIGHT_3_END_STABILITY=8.1000
6150
PRIMER_PAIR_3_COMPL_ANY=3.00
6151
PRIMER_PAIR_3_COMPL_END=0.00
6152
PRIMER_PRODUCT_SIZE_3=879
6153
PRIMER_PAIR_PENALTY_4=7.8470
6154
PRIMER_LEFT_4_PENALTY=3.142611
6155
PRIMER_RIGHT_4_PENALTY=4.704404
6156
PRIMER_LEFT_4_SEQUENCE=ATGCAGAAATACGAGAAACTGGA
6157
PRIMER_RIGHT_4_SEQUENCE=GCCTAGGGCGGACAGAAGT
6158
PRIMER_LEFT_4=24,23
6159
PRIMER_RIGHT_4=904,19
6160
PRIMER_LEFT_4_TM=60.143
6161
PRIMER_RIGHT_4_TM=61.704
6162
PRIMER_LEFT_4_GC_PERCENT=39.130
6163
PRIMER_RIGHT_4_GC_PERCENT=63.158
6164
PRIMER_LEFT_4_SELF_ANY=4.00
6165
PRIMER_RIGHT_4_SELF_ANY=6.00
6166
PRIMER_LEFT_4_SELF_END=0.00
6167
PRIMER_RIGHT_4_SELF_END=1.00
6168
PRIMER_LEFT_4_POSITION_PENALTY=0.000000
6169
PRIMER_RIGHT_4_POSITION_PENALTY=2.000000
6170
PRIMER_LEFT_4_END_STABILITY=8.2000
6171
PRIMER_RIGHT_4_END_STABILITY=6.4000
6172
PRIMER_PAIR_4_COMPL_ANY=3.00
6173
PRIMER_PAIR_4_COMPL_END=1.00
6174
PRIMER_PRODUCT_SIZE_4=881
6175
PRIMER_PAIR_PENALTY_5=7.9905
6176
PRIMER_LEFT_5_PENALTY=5.302203
6177
PRIMER_RIGHT_5_PENALTY=2.688260
6178
PRIMER_LEFT_5_SEQUENCE=ATGCAGAAATACGAGAAACTGGAA
6179
PRIMER_RIGHT_5_SEQUENCE=CCTAGGGCGGACAGAAGTC
6180
PRIMER_LEFT_5=24,24
6181
PRIMER_RIGHT_5=903,19
6182
PRIMER_LEFT_5_TM=61.302
6183
PRIMER_RIGHT_5_TM=59.812
6184
PRIMER_LEFT_5_GC_PERCENT=37.500
6185
PRIMER_RIGHT_5_GC_PERCENT=63.158
6186
PRIMER_LEFT_5_SELF_ANY=4.00
6187
PRIMER_RIGHT_5_SELF_ANY=6.00
6188
PRIMER_LEFT_5_SELF_END=1.00
6189
PRIMER_RIGHT_5_SELF_END=2.00
6190
PRIMER_LEFT_5_POSITION_PENALTY=0.000000
6191
PRIMER_RIGHT_5_POSITION_PENALTY=1.500000
6192
PRIMER_LEFT_5_END_STABILITY=8.5000
6193
PRIMER_RIGHT_5_END_STABILITY=6.4000
6194
PRIMER_PAIR_5_COMPL_ANY=3.00
6195
PRIMER_PAIR_5_COMPL_END=1.00
6196
PRIMER_PRODUCT_SIZE_5=880
6197
PRIMER_PAIR_PENALTY_6=8.2407
6198
PRIMER_LEFT_6_PENALTY=5.302203
6199
PRIMER_RIGHT_6_PENALTY=2.938464
6200
PRIMER_LEFT_6_SEQUENCE=ATGCAGAAATACGAGAAACTGGAA
6201
PRIMER_RIGHT_6_SEQUENCE=CTAGGGCGGACAGAAGTCG
6202
PRIMER_LEFT_6=24,24
6203
PRIMER_RIGHT_6=902,19
6204
PRIMER_LEFT_6_TM=61.302
6205
PRIMER_RIGHT_6_TM=60.938
6206
PRIMER_LEFT_6_GC_PERCENT=37.500
6207
PRIMER_RIGHT_6_GC_PERCENT=63.158
6208
PRIMER_LEFT_6_SELF_ANY=4.00
6209
PRIMER_RIGHT_6_SELF_ANY=4.00
6210
PRIMER_LEFT_6_SELF_END=1.00
6211
PRIMER_RIGHT_6_SELF_END=2.00
6212
PRIMER_LEFT_6_POSITION_PENALTY=0.000000
6213
PRIMER_RIGHT_6_POSITION_PENALTY=1.000000
6214
PRIMER_LEFT_6_END_STABILITY=8.5000
6215
PRIMER_RIGHT_6_END_STABILITY=8.1000
6216
PRIMER_PAIR_6_COMPL_ANY=3.00
6217
PRIMER_PAIR_6_COMPL_END=0.00
6218
PRIMER_PRODUCT_SIZE_6=879
6219
PRIMER_PAIR_PENALTY_7=8.2442
6220
PRIMER_LEFT_7_PENALTY=3.142611
6221
PRIMER_RIGHT_7_PENALTY=5.101542
6222
PRIMER_LEFT_7_SEQUENCE=ATGCAGAAATACGAGAAACTGGA
6223
PRIMER_RIGHT_7_SEQUENCE=TAGGGCGGACAGAAGTCGG
6224
PRIMER_LEFT_7=24,23
6225
PRIMER_RIGHT_7=901,19
6226
PRIMER_LEFT_7_TM=60.143
6227
PRIMER_RIGHT_7_TM=63.602
6228
PRIMER_LEFT_7_GC_PERCENT=39.130
6229
PRIMER_RIGHT_7_GC_PERCENT=63.158
6230
PRIMER_LEFT_7_SELF_ANY=4.00
6231
PRIMER_RIGHT_7_SELF_ANY=3.00
6232
PRIMER_LEFT_7_SELF_END=0.00
6233
PRIMER_RIGHT_7_SELF_END=2.00
6234
PRIMER_LEFT_7_POSITION_PENALTY=0.000000
6235
PRIMER_RIGHT_7_POSITION_PENALTY=0.500000
6236
PRIMER_LEFT_7_END_STABILITY=8.2000
6237
PRIMER_RIGHT_7_END_STABILITY=9.6000
6238
PRIMER_PAIR_7_COMPL_ANY=3.00
6239
PRIMER_PAIR_7_COMPL_END=0.00
6240
PRIMER_PRODUCT_SIZE_7=878
6241
PRIMER_PAIR_PENALTY_8=8.3017
6242
PRIMER_LEFT_8_PENALTY=3.597306
6243
PRIMER_RIGHT_8_PENALTY=4.704404
6244
PRIMER_LEFT_8_SEQUENCE=ATGCAGAAATACGAGAAACTGG
6245
PRIMER_RIGHT_8_SEQUENCE=GCCTAGGGCGGACAGAAGT
6246
PRIMER_LEFT_8=24,22
6247
PRIMER_RIGHT_8=904,19
6248
PRIMER_LEFT_8_TM=58.403
6249
PRIMER_RIGHT_8_TM=61.704
6250
PRIMER_LEFT_8_GC_PERCENT=40.909
6251
PRIMER_RIGHT_8_GC_PERCENT=63.158
6252
PRIMER_LEFT_8_SELF_ANY=4.00
6253
PRIMER_RIGHT_8_SELF_ANY=6.00
6254
PRIMER_LEFT_8_SELF_END=1.00
6255
PRIMER_RIGHT_8_SELF_END=1.00
6256
PRIMER_LEFT_8_POSITION_PENALTY=0.000000
6257
PRIMER_RIGHT_8_POSITION_PENALTY=2.000000
6258
PRIMER_LEFT_8_END_STABILITY=7.9000
6259
PRIMER_RIGHT_8_END_STABILITY=6.4000
6260
PRIMER_PAIR_8_COMPL_ANY=3.00
6261
PRIMER_PAIR_8_COMPL_END=1.00
6262
PRIMER_PRODUCT_SIZE_8=881
6263
PRIMER_PAIR_PENALTY_9=8.5177
6264
PRIMER_LEFT_9_PENALTY=3.142611
6265
PRIMER_RIGHT_9_PENALTY=5.375058
6266
PRIMER_LEFT_9_SEQUENCE=ATGCAGAAATACGAGAAACTGGA
6267
PRIMER_RIGHT_9_SEQUENCE=CTAGGGCGGACAGAAGTCGG
6268
PRIMER_LEFT_9=24,23
6269
PRIMER_RIGHT_9=902,20
6270
PRIMER_LEFT_9_TM=60.143
6271
PRIMER_RIGHT_9_TM=64.375
6272
PRIMER_LEFT_9_GC_PERCENT=39.130
6273
PRIMER_RIGHT_9_GC_PERCENT=65.000
6274
PRIMER_LEFT_9_SELF_ANY=4.00
6275
PRIMER_RIGHT_9_SELF_ANY=4.00
6276
PRIMER_LEFT_9_SELF_END=0.00
6277
PRIMER_RIGHT_9_SELF_END=2.00
6278
PRIMER_LEFT_9_POSITION_PENALTY=0.000000
6279
PRIMER_RIGHT_9_POSITION_PENALTY=1.000000
6280
PRIMER_LEFT_9_END_STABILITY=8.2000
6281
PRIMER_RIGHT_9_END_STABILITY=9.6000
6282
PRIMER_PAIR_9_COMPL_ANY=3.00
6283
PRIMER_PAIR_9_COMPL_END=0.00
6284
PRIMER_PRODUCT_SIZE_9=879
6285
PRIMER_PAIR_PENALTY_10=8.6110
6286
PRIMER_LEFT_10_PENALTY=3.142611
6287
PRIMER_RIGHT_10_PENALTY=5.468402
6288
PRIMER_LEFT_10_SEQUENCE=ATGCAGAAATACGAGAAACTGGA
6289
PRIMER_RIGHT_10_SEQUENCE=GCCTAGGGCGGACAGAAGTC
6290
PRIMER_LEFT_10=24,23
6291
PRIMER_RIGHT_10=904,20
6292
PRIMER_LEFT_10_TM=60.143
6293
PRIMER_RIGHT_10_TM=63.468
6294
PRIMER_LEFT_10_GC_PERCENT=39.130
6295
PRIMER_RIGHT_10_GC_PERCENT=65.000
6296
PRIMER_LEFT_10_SELF_ANY=4.00
6297
PRIMER_RIGHT_10_SELF_ANY=6.00
6298
PRIMER_LEFT_10_SELF_END=0.00
6299
PRIMER_RIGHT_10_SELF_END=2.00
6300
PRIMER_LEFT_10_POSITION_PENALTY=0.000000
6301
PRIMER_RIGHT_10_POSITION_PENALTY=2.000000
6302
PRIMER_LEFT_10_END_STABILITY=8.2000
6303
PRIMER_RIGHT_10_END_STABILITY=6.4000
6304
PRIMER_PAIR_10_COMPL_ANY=3.00
6305
PRIMER_PAIR_10_COMPL_END=2.00
6306
PRIMER_PRODUCT_SIZE_10=881
6307
PRIMER_PAIR_PENALTY_11=8.6988
6308
PRIMER_LEFT_11_PENALTY=3.597306
6309
PRIMER_RIGHT_11_PENALTY=5.101542
6310
PRIMER_LEFT_11_SEQUENCE=ATGCAGAAATACGAGAAACTGG
6311
PRIMER_RIGHT_11_SEQUENCE=TAGGGCGGACAGAAGTCGG
6312
PRIMER_LEFT_11=24,22
6313
PRIMER_RIGHT_11=901,19
6314
PRIMER_LEFT_11_TM=58.403
6315
PRIMER_RIGHT_11_TM=63.602
6316
PRIMER_LEFT_11_GC_PERCENT=40.909
6317
PRIMER_RIGHT_11_GC_PERCENT=63.158
6318
PRIMER_LEFT_11_SELF_ANY=4.00
6319
PRIMER_RIGHT_11_SELF_ANY=3.00
6320
PRIMER_LEFT_11_SELF_END=1.00
6321
PRIMER_RIGHT_11_SELF_END=2.00
6322
PRIMER_LEFT_11_POSITION_PENALTY=0.000000
6323
PRIMER_RIGHT_11_POSITION_PENALTY=0.500000
6324
PRIMER_LEFT_11_END_STABILITY=7.9000
6325
PRIMER_RIGHT_11_END_STABILITY=9.6000
6326
PRIMER_PAIR_11_COMPL_ANY=3.00
6327
PRIMER_PAIR_11_COMPL_END=0.00
6328
PRIMER_PRODUCT_SIZE_11=878
6329
PRIMER_PAIR_PENALTY_12=8.9724
6330
PRIMER_LEFT_12_PENALTY=3.597306
6331
PRIMER_RIGHT_12_PENALTY=5.375058
6332
PRIMER_LEFT_12_SEQUENCE=ATGCAGAAATACGAGAAACTGG
6333
PRIMER_RIGHT_12_SEQUENCE=CTAGGGCGGACAGAAGTCGG
6334
PRIMER_LEFT_12=24,22
6335
PRIMER_RIGHT_12=902,20
6336
PRIMER_LEFT_12_TM=58.403
6337
PRIMER_RIGHT_12_TM=64.375
6338
PRIMER_LEFT_12_GC_PERCENT=40.909
6339
PRIMER_RIGHT_12_GC_PERCENT=65.000
6340
PRIMER_LEFT_12_SELF_ANY=4.00
6341
PRIMER_RIGHT_12_SELF_ANY=4.00
6342
PRIMER_LEFT_12_SELF_END=1.00
6343
PRIMER_RIGHT_12_SELF_END=2.00
6344
PRIMER_LEFT_12_POSITION_PENALTY=0.000000
6345
PRIMER_RIGHT_12_POSITION_PENALTY=1.000000
6346
PRIMER_LEFT_12_END_STABILITY=7.9000
6347
PRIMER_RIGHT_12_END_STABILITY=9.6000
6348
PRIMER_PAIR_12_COMPL_ANY=3.00
6349
PRIMER_PAIR_12_COMPL_END=0.00
6350
PRIMER_PRODUCT_SIZE_12=879
6351
PRIMER_PAIR_PENALTY_13=9.0177
6352
PRIMER_LEFT_13_PENALTY=3.142611
6353
PRIMER_RIGHT_13_PENALTY=5.875058
6354
PRIMER_LEFT_13_SEQUENCE=ATGCAGAAATACGAGAAACTGGA
6355
PRIMER_RIGHT_13_SEQUENCE=CCTAGGGCGGACAGAAGTCG
6356
PRIMER_LEFT_13=24,23
6357
PRIMER_RIGHT_13=903,20
6358
PRIMER_LEFT_13_TM=60.143
6359
PRIMER_RIGHT_13_TM=64.375
6360
PRIMER_LEFT_13_GC_PERCENT=39.130
6361
PRIMER_RIGHT_13_GC_PERCENT=65.000
6362
PRIMER_LEFT_13_SELF_ANY=4.00
6363
PRIMER_RIGHT_13_SELF_ANY=6.00
6364
PRIMER_LEFT_13_SELF_END=0.00
6365
PRIMER_RIGHT_13_SELF_END=2.00
6366
PRIMER_LEFT_13_POSITION_PENALTY=0.000000
6367
PRIMER_RIGHT_13_POSITION_PENALTY=1.500000
6368
PRIMER_LEFT_13_END_STABILITY=8.2000
6369
PRIMER_RIGHT_13_END_STABILITY=8.1000
6370
PRIMER_PAIR_13_COMPL_ANY=3.00
6371
PRIMER_PAIR_13_COMPL_END=1.00
6372
PRIMER_PRODUCT_SIZE_13=880
6373
PRIMER_PAIR_PENALTY_14=9.0657
6374
PRIMER_LEFT_14_PENALTY=3.597306
6375
PRIMER_RIGHT_14_PENALTY=5.468402
6376
PRIMER_LEFT_14_SEQUENCE=ATGCAGAAATACGAGAAACTGG
6377
PRIMER_RIGHT_14_SEQUENCE=GCCTAGGGCGGACAGAAGTC
6378
PRIMER_LEFT_14=24,22
6379
PRIMER_RIGHT_14=904,20
6380
PRIMER_LEFT_14_TM=58.403
6381
PRIMER_RIGHT_14_TM=63.468
6382
PRIMER_LEFT_14_GC_PERCENT=40.909
6383
PRIMER_RIGHT_14_GC_PERCENT=65.000
6384
PRIMER_LEFT_14_SELF_ANY=4.00
6385
PRIMER_RIGHT_14_SELF_ANY=6.00
6386
PRIMER_LEFT_14_SELF_END=1.00
6387
PRIMER_RIGHT_14_SELF_END=2.00
6388
PRIMER_LEFT_14_POSITION_PENALTY=0.000000
6389
PRIMER_RIGHT_14_POSITION_PENALTY=2.000000
6390
PRIMER_LEFT_14_END_STABILITY=7.9000
6391
PRIMER_RIGHT_14_END_STABILITY=6.4000
6392
PRIMER_PAIR_14_COMPL_ANY=3.00
6393
PRIMER_PAIR_14_COMPL_END=1.00
6394
PRIMER_PRODUCT_SIZE_14=881
6395
PRIMER_PAIR_PENALTY_15=9.4724
6396
PRIMER_LEFT_15_PENALTY=3.597306
6397
PRIMER_RIGHT_15_PENALTY=5.875058
6398
PRIMER_LEFT_15_SEQUENCE=ATGCAGAAATACGAGAAACTGG
6399
PRIMER_RIGHT_15_SEQUENCE=CCTAGGGCGGACAGAAGTCG
6400
PRIMER_LEFT_15=24,22
6401
PRIMER_RIGHT_15=903,20
6402
PRIMER_LEFT_15_TM=58.403
6403
PRIMER_RIGHT_15_TM=64.375
6404
PRIMER_LEFT_15_GC_PERCENT=40.909
6405
PRIMER_RIGHT_15_GC_PERCENT=65.000
6406
PRIMER_LEFT_15_SELF_ANY=4.00
6407
PRIMER_RIGHT_15_SELF_ANY=6.00
6408
PRIMER_LEFT_15_SELF_END=1.00
6409
PRIMER_RIGHT_15_SELF_END=2.00
6410
PRIMER_LEFT_15_POSITION_PENALTY=0.000000
6411
PRIMER_RIGHT_15_POSITION_PENALTY=1.500000
6412
PRIMER_LEFT_15_END_STABILITY=7.9000
6413
PRIMER_RIGHT_15_END_STABILITY=8.1000
6414
PRIMER_PAIR_15_COMPL_ANY=3.00
6415
PRIMER_PAIR_15_COMPL_END=0.00
6416
PRIMER_PRODUCT_SIZE_15=880
6417
PRIMER_PAIR_PENALTY_16=10.0066
6418
PRIMER_LEFT_16_PENALTY=5.302203
6419
PRIMER_RIGHT_16_PENALTY=4.704404
6420
PRIMER_LEFT_16_SEQUENCE=ATGCAGAAATACGAGAAACTGGAA
6421
PRIMER_RIGHT_16_SEQUENCE=GCCTAGGGCGGACAGAAGT
6422
PRIMER_LEFT_16=24,24
6423
PRIMER_RIGHT_16=904,19
6424
PRIMER_LEFT_16_TM=61.302
6425
PRIMER_RIGHT_16_TM=61.704
6426
PRIMER_LEFT_16_GC_PERCENT=37.500
6427
PRIMER_RIGHT_16_GC_PERCENT=63.158
6428
PRIMER_LEFT_16_SELF_ANY=4.00
6429
PRIMER_RIGHT_16_SELF_ANY=6.00
6430
PRIMER_LEFT_16_SELF_END=1.00
6431
PRIMER_RIGHT_16_SELF_END=1.00
6432
PRIMER_LEFT_16_POSITION_PENALTY=0.000000
6433
PRIMER_RIGHT_16_POSITION_PENALTY=2.000000
6434
PRIMER_LEFT_16_END_STABILITY=8.5000
6435
PRIMER_RIGHT_16_END_STABILITY=6.4000
6436
PRIMER_PAIR_16_COMPL_ANY=3.00
6437
PRIMER_PAIR_16_COMPL_END=1.00
6438
PRIMER_PRODUCT_SIZE_16=881
6439
PRIMER_PAIR_PENALTY_17=10.0443
6440
PRIMER_LEFT_17_PENALTY=7.356069
6441
PRIMER_RIGHT_17_PENALTY=2.688260
6442
PRIMER_LEFT_17_SEQUENCE=ATGCAGAAATACGAGAAACTGGAAA
6443
PRIMER_RIGHT_17_SEQUENCE=CCTAGGGCGGACAGAAGTC
6444
PRIMER_LEFT_17=24,25
6445
PRIMER_RIGHT_17=903,19
6446
PRIMER_LEFT_17_TM=62.356
6447
PRIMER_RIGHT_17_TM=59.812
6448
PRIMER_LEFT_17_GC_PERCENT=36.000
6449
PRIMER_RIGHT_17_GC_PERCENT=63.158
6450
PRIMER_LEFT_17_SELF_ANY=4.00
6451
PRIMER_RIGHT_17_SELF_ANY=6.00
6452
PRIMER_LEFT_17_SELF_END=0.00
6453
PRIMER_RIGHT_17_SELF_END=2.00
6454
PRIMER_LEFT_17_POSITION_PENALTY=0.000000
6455
PRIMER_RIGHT_17_POSITION_PENALTY=1.500000
6456
PRIMER_LEFT_17_END_STABILITY=8.5000
6457
PRIMER_RIGHT_17_END_STABILITY=6.4000
6458
PRIMER_PAIR_17_COMPL_ANY=3.00
6459
PRIMER_PAIR_17_COMPL_END=0.00
6460
PRIMER_PRODUCT_SIZE_17=880
6461
PRIMER_PAIR_PENALTY_18=10.2945
6462
PRIMER_LEFT_18_PENALTY=7.356069
6463
PRIMER_RIGHT_18_PENALTY=2.938464
6464
PRIMER_LEFT_18_SEQUENCE=ATGCAGAAATACGAGAAACTGGAAA
6465
PRIMER_RIGHT_18_SEQUENCE=CTAGGGCGGACAGAAGTCG
6466
PRIMER_LEFT_18=24,25
6467
PRIMER_RIGHT_18=902,19
6468
PRIMER_LEFT_18_TM=62.356
6469
PRIMER_RIGHT_18_TM=60.938
6470
PRIMER_LEFT_18_GC_PERCENT=36.000
6471
PRIMER_RIGHT_18_GC_PERCENT=63.158
6472
PRIMER_LEFT_18_SELF_ANY=4.00
6473
PRIMER_RIGHT_18_SELF_ANY=4.00
6474
PRIMER_LEFT_18_SELF_END=0.00
6475
PRIMER_RIGHT_18_SELF_END=2.00
6476
PRIMER_LEFT_18_POSITION_PENALTY=0.000000
6477
PRIMER_RIGHT_18_POSITION_PENALTY=1.000000
6478
PRIMER_LEFT_18_END_STABILITY=8.5000
6479
PRIMER_RIGHT_18_END_STABILITY=8.1000
6480
PRIMER_PAIR_18_COMPL_ANY=3.00
6481
PRIMER_PAIR_18_COMPL_END=0.00
6482
PRIMER_PRODUCT_SIZE_18=879
6483
PRIMER_PAIR_PENALTY_19=10.4037
6484
PRIMER_LEFT_19_PENALTY=5.302203
6485
PRIMER_RIGHT_19_PENALTY=5.101542
6486
PRIMER_LEFT_19_SEQUENCE=ATGCAGAAATACGAGAAACTGGAA
6487
PRIMER_RIGHT_19_SEQUENCE=TAGGGCGGACAGAAGTCGG
6488
PRIMER_LEFT_19=24,24
6489
PRIMER_RIGHT_19=901,19
6490
PRIMER_LEFT_19_TM=61.302
6491
PRIMER_RIGHT_19_TM=63.602
6492
PRIMER_LEFT_19_GC_PERCENT=37.500
6493
PRIMER_RIGHT_19_GC_PERCENT=63.158
6494
PRIMER_LEFT_19_SELF_ANY=4.00
6495
PRIMER_RIGHT_19_SELF_ANY=3.00
6496
PRIMER_LEFT_19_SELF_END=1.00
6497
PRIMER_RIGHT_19_SELF_END=2.00
6498
PRIMER_LEFT_19_POSITION_PENALTY=0.000000
6499
PRIMER_RIGHT_19_POSITION_PENALTY=0.500000
6500
PRIMER_LEFT_19_END_STABILITY=8.5000
6501
PRIMER_RIGHT_19_END_STABILITY=9.6000
6502
PRIMER_PAIR_19_COMPL_ANY=3.00
6503
PRIMER_PAIR_19_COMPL_END=0.00
6504
PRIMER_PRODUCT_SIZE_19=878
6505
=
6506
PRIMER_SEQUENCE_ID=L34673/HIP116
6507
PRIMER_COMMENT=Human ATPase, DNA-binding protein (HIP116) mRNA, 3' end. CDS=178..3207
6508
PRIMER_START_CODON_POSITION=177
6509
SEQUENCE=ATTCCCGGGGTCTGACTGGACTCGCGGCGACTTACCTTTCAGTCGTGCGCTCCTGATCCGGCGCTCGGAATTTGTCCCCGGCTTCAGGGCTGCGGGGCCTGGAAGGAGGCGTATCGAGGCGGCTCGAAAACGATCCAGGGGAGCCGAGGCGCTCCTCTTGTCATCCCACTCAGCGCCATGTCCTGGATGTTCAAGAGGGATCCAGTTTGGAAGTACTTGCAGACTGTCCAGTATGGAGTTCATGGAAATTTTCCACGCCTCTCATATCCAACTTTCTTTCCACGTTTTGAATTCCAAGATGTTATCCCTCCAGATGACTTTCTAACTAGTGATGAAGAAGTAGATTCCGTTTTATTTGGAAGTTTGAGAGGTCATGTGGTTGGACTACGCTATTACACGGGAGTAGTTAATAATAATGAAATGGTTGCATTACAACGAGATCCTAATAACCCTTATGATAAGAATGCAATTAAAGTAAACAATGTGAATGGAAATCAAGTTGGCCATTTAAAGAAAGAGCTTGCAGGTGCTTTGGCCTATATCATGGACAACAAATTGGCACAAATTGAAGGGGTAGTTCCTTTTGGTGCAAACAATGCTTTTACCATGCCTCTGCATATGACTTTTTGGGGAAAAGAAGAAAATAGAAAAGCGGTTTCAGATCAGTTGAAGAAACATGGATTTAAATTGGGTCCTGCACCAAAAACTTTAGGATTCAATTTGGAAAGTGGTTGGGGCTCTGGAAGAGCTGGACCAAGCTATAGTATGCCAGTGCATGCTGCAGTACAGATGACAACTGAACAGCTTAAAACAGAATTTGACAAATTGTTTGAAGATTTAAAAGAAGATGATAAAACCCATGAAATGGAACCAGCTGAGGCTATTGAAACACCACTGCTTCCACATCAAAAACAAGCTCTAGCTTGGATGGTGTCACGGGAAAATAGCAAAGAACTTCCACCATTCTGGGAACAGCGAAATGACTTATACTATAACACAATAACAAATTTTTCTGAGAAGGACCGACCAGAAAATGTCCATGGAGGAATTTTAGCTGATGATATGGGTTTGGGTAAAACTCTTACAGCCATTGCAGTAATCCTTACCAACTTCCATGATGGCAGACCTCTTCCTATTGAAAGAGTTAAAAAGAATCTACTGAAGAAGGAATATAATGTTAACGATGACTCTATGAAACTTGGAGGAAACAATACCAGTGAAAAGGCAGATGGACTAAGCAAAGACGCATCTAGATGTAGTGAACAACCCAGTATTTCAGATATCAAGGAGAAGAGTAAGTTTCGCATGTCAGAATTGTCTACGTCCCGCCCCAAAAGAAGAAAAACTGCTGTCCAGTACATAGAAAGCAGTGATTCAGAGGAAATTGAAACAAGTGAATTGCCGCAGAAAATGAAAGGCAAACTGAAAAATGTACAGTCTGAAACTAAAGGCAGGGCGAAAGCAGGATCTTCTAAGGTTATAGAAGATGTGGCATTTGCATGTGCATTAACTTCATCTGTTCCTACAACAAAAAAGAAAATGTTGAAAAAGGGAGCTTGTGCAGTGGAGGGGTCAAAGAAAACTGATGTTGAGGAGAGACCAAGAACAACACTGATCATCTGTCCGCTTTCTGTGTTAAGCAACTGGATTGACCAGTTTGGACAACATATAAAATCAGATGTACACTTGAATTTTTATGTTTATTATGGTCCTGATCGTATTAGAGAACCGGCCTTACTTTCAAAACAGGATATTGTTTTGACTACGTATAATATTTTAACTCATGACTATGGAACTAAAGGAGATAGTCCATTACATAGCATAAGGTGGCTAAGAGTGATCCTGGATGAAGGACATGCCATACGAAATCCAAATGCTCAGCAGACAAAAGCTGTACTTGACTTAGAATCAGAAAGAAGATGGGTTTTGACAGGTACTCCAATCCAGAATTCTTTAAAGGACTTGTGGTCTCTTCTTTCCTTTTTAAAACTTAAACCATTTATTGATAGAGAATGGTGGCATAGAACAATACAGCGTCCTGTCACAATGGGAGATGAAGGAGGACTTAGGCGTTTACAGTCCCTAATTAAAAATATTACACTTAGAAGAACAAAGACAAGCAAAATTAAAGGAAAACCTGTTTTGGAGTTACCAGAACGTAAAGTATTTATTCAGCACATTACACTTTCAGATGAAGAGAGAAAGATTTATCAGTCTGTGAAAAATGAAGGCAGAGCCACTATTGGAAGGTATTTTAATGAAGGGACTGTCCTGGCACATTATGCAGATGTCCTGGGTCTTTTGCTTAGACTGCGGCAAATTTGTTGCCATACTTACCTTCTTACAAATGCAGTGTCTTCCAATGGCCCCTCAGGAAATGATACACCTGAAGAACTGAGAAAGAAGTTAATAAGGAAGATGAAGTTAATTCTGAGCTCAGGTTCAGATGAGGAATGTGCAATTTGCCTGGATTCTTTAACAGTTCCTGTGATAACACATTGTGCACATGTATTTTGTAAACCCTGTATTTGCCAAGTCATTCAGAATGAGCAGCCACATGCTAAATGCCCTTTATGCAGAAATGATATACATGAAGATAATTTATTAGAATGTCCTCCAGAAGAATTAGCACGTGACAGTGAGAAAAAGTCTGATATGGAATGGACATCCAGTTCAAAGATTAATGCGCTAATGCACGCATTGACTGACTTAAGAAAGAAGAATCCCAACATAAAAAGTTTGGTTGTTTCTCAGTTTACAACATTCCTGTCTTTAATAGAAATACCACTTAAAGCCTCTGGATTTGTGTTTACTCGTTTGGATGGTTCCATGGCCCAAAAGAAAAGAGTTGAATCAATTCAGTGTTTTCAAAACACTGAAGCAGGATCTCCAACTATAATGCTTCTGTCCTTAAAAGCAGGTGGAGTTGGTTTGAATCTGTCTGCAGCTTCTCGAGTGTTTTTAATGGATCCAGCCTGGAATCCTGCTGCTGAAGATCAGTGCTTTGACAGATGCCATAGACTTGGTCAGAAGCAAGAAGTTATCATCACAAAATTCATTGTAAAGGACTCTGTTGAAGAAAATATGCTGAAAATACAAAACAAAAAGAGAGAACTTGCAGCAGGAGCCTTTGGAACTAAAAAACCAAATGCTGACGAAATGAAACAAGCCAAAATTAATGAAATCAGAACATTAATTGACTTATAATTTGTGGGATTTTAGTAAGAAGACTACTATATGTGAGAGGCGTGATATCTGGATGGAAGTTGGGCTGGATGATCTCCAAAGTCGTTTCAACTCTTAAAGACATCTTAATCCTGAATGTAAACAATTGTTATGTGTTTAGAATCAGAATTTGATTTTGAACTTGAGTAATTCATCCTTACAGCTATCTGTAGAATTAGTCATCTTTTTTCTT
6510
PRIMER_LEFT_EXPLAIN=considered 29448, would not amplify any of the ORF 20059, GC content failed 202, low tm 3593, high tm 1850, high any compl 5, high end compl 23, long poly-x seq 41,high 3' stability 65, ok 3610
6511
PRIMER_RIGHT_EXPLAIN=considered 29262, GC content failed 608, low tm 11948, high tm 5429, high end compl 9, long poly-x seq 56,high 3' stability 79, ok 11133
6512
PRIMER_PAIR_EXPLAIN=considered 49, ok 49
6513
PRIMER_STOP_CODON_POSITION=3204
6514
PRIMER_PAIR_PENALTY=15.9507
6515
PRIMER_LEFT_PENALTY=1.996678
6516
PRIMER_RIGHT_PENALTY=13.954041
6517
PRIMER_LEFT_SEQUENCE=ATGTCCTGGATGTTCAAGAGG
6518
PRIMER_RIGHT_SEQUENCE=CCCACAAATTATAAGTCAATTAATGTT
6519
PRIMER_LEFT=177,21
6520
PRIMER_RIGHT=3214,27
6521
PRIMER_LEFT_TM=59.003
6522
PRIMER_RIGHT_TM=58.046
6523
PRIMER_LEFT_GC_PERCENT=47.619
6524
PRIMER_RIGHT_GC_PERCENT=25.926
6525
PRIMER_LEFT_SELF_ANY=5.00
6526
PRIMER_RIGHT_SELF_ANY=6.00
6527
PRIMER_LEFT_SELF_END=0.00
6528
PRIMER_RIGHT_SELF_END=3.00
6529
PRIMER_LEFT_POSITION_PENALTY=0.000000
6530
PRIMER_RIGHT_POSITION_PENALTY=5.000000
6531
PRIMER_LEFT_END_STABILITY=7.9000
6532
PRIMER_RIGHT_END_STABILITY=6.6000
6533
PRIMER_PAIR_COMPL_ANY=3.00
6534
PRIMER_PAIR_COMPL_END=0.00
6535
PRIMER_PRODUCT_SIZE=3038
6536
PRIMER_PAIR_PENALTY_1=18.4775
6537
PRIMER_LEFT_1_PENALTY=4.523430
6538
PRIMER_RIGHT_1_PENALTY=13.954041
6539
PRIMER_LEFT_1_SEQUENCE=ATGTCCTGGATGTTCAAGAGGG
6540
PRIMER_RIGHT_1_SEQUENCE=CCCACAAATTATAAGTCAATTAATGTT
6541
PRIMER_LEFT_1=177,22
6542
PRIMER_RIGHT_1=3214,27
6543
PRIMER_LEFT_1_TM=62.523
6544
PRIMER_RIGHT_1_TM=58.046
6545
PRIMER_LEFT_1_GC_PERCENT=50.000
6546
PRIMER_RIGHT_1_GC_PERCENT=25.926
6547
PRIMER_LEFT_1_SELF_ANY=5.00
6548
PRIMER_RIGHT_1_SELF_ANY=6.00
6549
PRIMER_LEFT_1_SELF_END=0.00
6550
PRIMER_RIGHT_1_SELF_END=3.00
6551
PRIMER_LEFT_1_POSITION_PENALTY=0.000000
6552
PRIMER_RIGHT_1_POSITION_PENALTY=5.000000
6553
PRIMER_LEFT_1_END_STABILITY=9.4000
6554
PRIMER_RIGHT_1_END_STABILITY=6.6000
6555
PRIMER_PAIR_1_COMPL_ANY=3.00
6556
PRIMER_PAIR_1_COMPL_END=0.00
6557
PRIMER_PRODUCT_SIZE_1=3038
6558
PRIMER_PAIR_PENALTY_2=19.3757
6559
PRIMER_LEFT_2_PENALTY=1.996678
6560
PRIMER_RIGHT_2_PENALTY=17.378993
6561
PRIMER_LEFT_2_SEQUENCE=ATGTCCTGGATGTTCAAGAGG
6562
PRIMER_RIGHT_2_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAA
6563
PRIMER_LEFT_2=177,21
6564
PRIMER_RIGHT_2=3221,26
6565
PRIMER_LEFT_2_TM=59.003
6566
PRIMER_RIGHT_2_TM=57.121
6567
PRIMER_LEFT_2_GC_PERCENT=47.619
6568
PRIMER_RIGHT_2_GC_PERCENT=26.923
6569
PRIMER_LEFT_2_SELF_ANY=5.00
6570
PRIMER_RIGHT_2_SELF_ANY=6.00
6571
PRIMER_LEFT_2_SELF_END=0.00
6572
PRIMER_RIGHT_2_SELF_END=0.00
6573
PRIMER_LEFT_2_POSITION_PENALTY=0.000000
6574
PRIMER_RIGHT_2_POSITION_PENALTY=8.500000
6575
PRIMER_LEFT_2_END_STABILITY=7.9000
6576
PRIMER_RIGHT_2_END_STABILITY=6.7000
6577
PRIMER_PAIR_2_COMPL_ANY=5.00
6578
PRIMER_PAIR_2_COMPL_END=0.00
6579
PRIMER_PRODUCT_SIZE_2=3045
6580
PRIMER_PAIR_PENALTY_3=20.0477
6581
PRIMER_LEFT_3_PENALTY=1.996678
6582
PRIMER_RIGHT_3_PENALTY=18.051002
6583
PRIMER_LEFT_3_SEQUENCE=ATGTCCTGGATGTTCAAGAGG
6584
PRIMER_RIGHT_3_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAAT
6585
PRIMER_LEFT_3=177,21
6586
PRIMER_RIGHT_3=3221,27
6587
PRIMER_LEFT_3_TM=59.003
6588
PRIMER_RIGHT_3_TM=57.449
6589
PRIMER_LEFT_3_GC_PERCENT=47.619
6590
PRIMER_RIGHT_3_GC_PERCENT=25.926
6591
PRIMER_LEFT_3_SELF_ANY=5.00
6592
PRIMER_RIGHT_3_SELF_ANY=6.00
6593
PRIMER_LEFT_3_SELF_END=0.00
6594
PRIMER_RIGHT_3_SELF_END=2.00
6595
PRIMER_LEFT_3_POSITION_PENALTY=0.000000
6596
PRIMER_RIGHT_3_POSITION_PENALTY=8.500000
6597
PRIMER_LEFT_3_END_STABILITY=7.9000
6598
PRIMER_RIGHT_3_END_STABILITY=6.9000
6599
PRIMER_PAIR_3_COMPL_ANY=5.00
6600
PRIMER_PAIR_3_COMPL_END=0.00
6601
PRIMER_PRODUCT_SIZE_3=3045
6602
PRIMER_PAIR_PENALTY_4=20.1564
6603
PRIMER_LEFT_4_PENALTY=1.996678
6604
PRIMER_RIGHT_4_PENALTY=18.159733
6605
PRIMER_LEFT_4_SEQUENCE=ATGTCCTGGATGTTCAAGAGG
6606
PRIMER_RIGHT_4_SEQUENCE=ACTAAAATCCCACAAATTATAAGTCAA
6607
PRIMER_LEFT_4=177,21
6608
PRIMER_RIGHT_4=3222,27
6609
PRIMER_LEFT_4_TM=59.003
6610
PRIMER_RIGHT_4_TM=57.840
6611
PRIMER_LEFT_4_GC_PERCENT=47.619
6612
PRIMER_RIGHT_4_GC_PERCENT=25.926
6613
PRIMER_LEFT_4_SELF_ANY=5.00
6614
PRIMER_RIGHT_4_SELF_ANY=6.00
6615
PRIMER_LEFT_4_SELF_END=0.00
6616
PRIMER_RIGHT_4_SELF_END=0.00
6617
PRIMER_LEFT_4_POSITION_PENALTY=0.000000
6618
PRIMER_RIGHT_4_POSITION_PENALTY=9.000000
6619
PRIMER_LEFT_4_END_STABILITY=7.9000
6620
PRIMER_RIGHT_4_END_STABILITY=6.7000
6621
PRIMER_PAIR_4_COMPL_ANY=5.00
6622
PRIMER_PAIR_4_COMPL_END=0.00
6623
PRIMER_PRODUCT_SIZE_4=3046
6624
PRIMER_PAIR_PENALTY_5=21.1472
6625
PRIMER_LEFT_5_PENALTY=7.193142
6626
PRIMER_RIGHT_5_PENALTY=13.954041
6627
PRIMER_LEFT_5_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGA
6628
PRIMER_RIGHT_5_SEQUENCE=CCCACAAATTATAAGTCAATTAATGTT
6629
PRIMER_LEFT_5=177,23
6630
PRIMER_RIGHT_5=3214,27
6631
PRIMER_LEFT_5_TM=64.193
6632
PRIMER_RIGHT_5_TM=58.046
6633
PRIMER_LEFT_5_GC_PERCENT=47.826
6634
PRIMER_RIGHT_5_GC_PERCENT=25.926
6635
PRIMER_LEFT_5_SELF_ANY=5.00
6636
PRIMER_RIGHT_5_SELF_ANY=6.00
6637
PRIMER_LEFT_5_SELF_END=0.00
6638
PRIMER_RIGHT_5_SELF_END=3.00
6639
PRIMER_LEFT_5_POSITION_PENALTY=0.000000
6640
PRIMER_RIGHT_5_POSITION_PENALTY=5.000000
6641
PRIMER_LEFT_5_END_STABILITY=9.4000
6642
PRIMER_RIGHT_5_END_STABILITY=6.6000
6643
PRIMER_PAIR_5_COMPL_ANY=3.00
6644
PRIMER_PAIR_5_COMPL_END=1.00
6645
PRIMER_PRODUCT_SIZE_5=3038
6646
PRIMER_PAIR_PENALTY_6=21.9024
6647
PRIMER_LEFT_6_PENALTY=4.523430
6648
PRIMER_RIGHT_6_PENALTY=17.378993
6649
PRIMER_LEFT_6_SEQUENCE=ATGTCCTGGATGTTCAAGAGGG
6650
PRIMER_RIGHT_6_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAA
6651
PRIMER_LEFT_6=177,22
6652
PRIMER_RIGHT_6=3221,26
6653
PRIMER_LEFT_6_TM=62.523
6654
PRIMER_RIGHT_6_TM=57.121
6655
PRIMER_LEFT_6_GC_PERCENT=50.000
6656
PRIMER_RIGHT_6_GC_PERCENT=26.923
6657
PRIMER_LEFT_6_SELF_ANY=5.00
6658
PRIMER_RIGHT_6_SELF_ANY=6.00
6659
PRIMER_LEFT_6_SELF_END=0.00
6660
PRIMER_RIGHT_6_SELF_END=0.00
6661
PRIMER_LEFT_6_POSITION_PENALTY=0.000000
6662
PRIMER_RIGHT_6_POSITION_PENALTY=8.500000
6663
PRIMER_LEFT_6_END_STABILITY=9.4000
6664
PRIMER_RIGHT_6_END_STABILITY=6.7000
6665
PRIMER_PAIR_6_COMPL_ANY=5.00
6666
PRIMER_PAIR_6_COMPL_END=0.00
6667
PRIMER_PRODUCT_SIZE_6=3045
6668
PRIMER_PAIR_PENALTY_7=22.1908
6669
PRIMER_LEFT_7_PENALTY=8.236744
6670
PRIMER_RIGHT_7_PENALTY=13.954041
6671
PRIMER_LEFT_7_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGAT
6672
PRIMER_RIGHT_7_SEQUENCE=CCCACAAATTATAAGTCAATTAATGTT
6673
PRIMER_LEFT_7=177,24
6674
PRIMER_RIGHT_7=3214,27
6675
PRIMER_LEFT_7_TM=64.237
6676
PRIMER_RIGHT_7_TM=58.046
6677
PRIMER_LEFT_7_GC_PERCENT=45.833
6678
PRIMER_RIGHT_7_GC_PERCENT=25.926
6679
PRIMER_LEFT_7_SELF_ANY=5.00
6680
PRIMER_RIGHT_7_SELF_ANY=6.00
6681
PRIMER_LEFT_7_SELF_END=2.00
6682
PRIMER_RIGHT_7_SELF_END=3.00
6683
PRIMER_LEFT_7_POSITION_PENALTY=0.000000
6684
PRIMER_RIGHT_7_POSITION_PENALTY=5.000000
6685
PRIMER_LEFT_7_END_STABILITY=9.3000
6686
PRIMER_RIGHT_7_END_STABILITY=6.6000
6687
PRIMER_PAIR_7_COMPL_ANY=3.00
6688
PRIMER_PAIR_7_COMPL_END=1.00
6689
PRIMER_PRODUCT_SIZE_7=3038
6690
PRIMER_PAIR_PENALTY_8=22.5744
6691
PRIMER_LEFT_8_PENALTY=4.523430
6692
PRIMER_RIGHT_8_PENALTY=18.051002
6693
PRIMER_LEFT_8_SEQUENCE=ATGTCCTGGATGTTCAAGAGGG
6694
PRIMER_RIGHT_8_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAAT
6695
PRIMER_LEFT_8=177,22
6696
PRIMER_RIGHT_8=3221,27
6697
PRIMER_LEFT_8_TM=62.523
6698
PRIMER_RIGHT_8_TM=57.449
6699
PRIMER_LEFT_8_GC_PERCENT=50.000
6700
PRIMER_RIGHT_8_GC_PERCENT=25.926
6701
PRIMER_LEFT_8_SELF_ANY=5.00
6702
PRIMER_RIGHT_8_SELF_ANY=6.00
6703
PRIMER_LEFT_8_SELF_END=0.00
6704
PRIMER_RIGHT_8_SELF_END=2.00
6705
PRIMER_LEFT_8_POSITION_PENALTY=0.000000
6706
PRIMER_RIGHT_8_POSITION_PENALTY=8.500000
6707
PRIMER_LEFT_8_END_STABILITY=9.4000
6708
PRIMER_RIGHT_8_END_STABILITY=6.9000
6709
PRIMER_PAIR_8_COMPL_ANY=5.00
6710
PRIMER_PAIR_8_COMPL_END=0.00
6711
PRIMER_PRODUCT_SIZE_8=3045
6712
PRIMER_PAIR_PENALTY_9=22.6832
6713
PRIMER_LEFT_9_PENALTY=4.523430
6714
PRIMER_RIGHT_9_PENALTY=18.159733
6715
PRIMER_LEFT_9_SEQUENCE=ATGTCCTGGATGTTCAAGAGGG
6716
PRIMER_RIGHT_9_SEQUENCE=ACTAAAATCCCACAAATTATAAGTCAA
6717
PRIMER_LEFT_9=177,22
6718
PRIMER_RIGHT_9=3222,27
6719
PRIMER_LEFT_9_TM=62.523
6720
PRIMER_RIGHT_9_TM=57.840
6721
PRIMER_LEFT_9_GC_PERCENT=50.000
6722
PRIMER_RIGHT_9_GC_PERCENT=25.926
6723
PRIMER_LEFT_9_SELF_ANY=5.00
6724
PRIMER_RIGHT_9_SELF_ANY=6.00
6725
PRIMER_LEFT_9_SELF_END=0.00
6726
PRIMER_RIGHT_9_SELF_END=0.00
6727
PRIMER_LEFT_9_POSITION_PENALTY=0.000000
6728
PRIMER_RIGHT_9_POSITION_PENALTY=9.000000
6729
PRIMER_LEFT_9_END_STABILITY=9.4000
6730
PRIMER_RIGHT_9_END_STABILITY=6.7000
6731
PRIMER_PAIR_9_COMPL_ANY=5.00
6732
PRIMER_PAIR_9_COMPL_END=0.00
6733
PRIMER_PRODUCT_SIZE_9=3046
6734
PRIMER_PAIR_PENALTY_10=24.2545
6735
PRIMER_LEFT_10_PENALTY=1.996678
6736
PRIMER_RIGHT_10_PENALTY=22.257817
6737
PRIMER_LEFT_10_SEQUENCE=ATGTCCTGGATGTTCAAGAGG
6738
PRIMER_RIGHT_10_SEQUENCE=TCTTCTTACTAAAATCCCACAAATTAT
6739
PRIMER_LEFT_10=177,21
6740
PRIMER_RIGHT_10=3229,27
6741
PRIMER_LEFT_10_TM=59.003
6742
PRIMER_RIGHT_10_TM=57.242
6743
PRIMER_LEFT_10_GC_PERCENT=47.619
6744
PRIMER_RIGHT_10_GC_PERCENT=25.926
6745
PRIMER_LEFT_10_SELF_ANY=5.00
6746
PRIMER_RIGHT_10_SELF_ANY=5.00
6747
PRIMER_LEFT_10_SELF_END=0.00
6748
PRIMER_RIGHT_10_SELF_END=3.00
6749
PRIMER_LEFT_10_POSITION_PENALTY=0.000000
6750
PRIMER_RIGHT_10_POSITION_PENALTY=12.500000
6751
PRIMER_LEFT_10_END_STABILITY=7.9000
6752
PRIMER_RIGHT_10_END_STABILITY=5.8000
6753
PRIMER_PAIR_10_COMPL_ANY=5.00
6754
PRIMER_PAIR_10_COMPL_END=3.00
6755
PRIMER_PRODUCT_SIZE_10=3053
6756
PRIMER_PAIR_PENALTY_11=24.2579
6757
PRIMER_LEFT_11_PENALTY=1.996678
6758
PRIMER_RIGHT_11_PENALTY=22.261240
6759
PRIMER_LEFT_11_SEQUENCE=ATGTCCTGGATGTTCAAGAGG
6760
PRIMER_RIGHT_11_SEQUENCE=AGTCTTCTTACTAAAATCCCACAAAT
6761
PRIMER_LEFT_11=177,21
6762
PRIMER_RIGHT_11=3231,26
6763
PRIMER_LEFT_11_TM=59.003
6764
PRIMER_RIGHT_11_TM=57.239
6765
PRIMER_LEFT_11_GC_PERCENT=47.619
6766
PRIMER_RIGHT_11_GC_PERCENT=30.769
6767
PRIMER_LEFT_11_SELF_ANY=5.00
6768
PRIMER_RIGHT_11_SELF_ANY=5.00
6769
PRIMER_LEFT_11_SELF_END=0.00
6770
PRIMER_RIGHT_11_SELF_END=2.00
6771
PRIMER_LEFT_11_POSITION_PENALTY=0.000000
6772
PRIMER_RIGHT_11_POSITION_PENALTY=13.500000
6773
PRIMER_LEFT_11_END_STABILITY=7.9000
6774
PRIMER_RIGHT_11_END_STABILITY=7.2000
6775
PRIMER_PAIR_11_COMPL_ANY=5.00
6776
PRIMER_PAIR_11_COMPL_END=3.00
6777
PRIMER_PRODUCT_SIZE_11=3055
6778
PRIMER_PAIR_PENALTY_12=24.3639
6779
PRIMER_LEFT_12_PENALTY=1.996678
6780
PRIMER_RIGHT_12_PENALTY=22.367225
6781
PRIMER_LEFT_12_SEQUENCE=ATGTCCTGGATGTTCAAGAGG
6782
PRIMER_RIGHT_12_SEQUENCE=GTCTTCTTACTAAAATCCCACAAATTA
6783
PRIMER_LEFT_12=177,21
6784
PRIMER_RIGHT_12=3230,27
6785
PRIMER_LEFT_12_TM=59.003
6786
PRIMER_RIGHT_12_TM=57.633
6787
PRIMER_LEFT_12_GC_PERCENT=47.619
6788
PRIMER_RIGHT_12_GC_PERCENT=29.630
6789
PRIMER_LEFT_12_SELF_ANY=5.00
6790
PRIMER_RIGHT_12_SELF_ANY=5.00
6791
PRIMER_LEFT_12_SELF_END=0.00
6792
PRIMER_RIGHT_12_SELF_END=2.00
6793
PRIMER_LEFT_12_POSITION_PENALTY=0.000000
6794
PRIMER_RIGHT_12_POSITION_PENALTY=13.000000
6795
PRIMER_LEFT_12_END_STABILITY=7.9000
6796
PRIMER_RIGHT_12_END_STABILITY=6.2000
6797
PRIMER_PAIR_12_COMPL_ANY=5.00
6798
PRIMER_PAIR_12_COMPL_END=3.00
6799
PRIMER_PRODUCT_SIZE_12=3054
6800
PRIMER_PAIR_PENALTY_13=24.5721
6801
PRIMER_LEFT_13_PENALTY=7.193142
6802
PRIMER_RIGHT_13_PENALTY=17.378993
6803
PRIMER_LEFT_13_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGA
6804
PRIMER_RIGHT_13_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAA
6805
PRIMER_LEFT_13=177,23
6806
PRIMER_RIGHT_13=3221,26
6807
PRIMER_LEFT_13_TM=64.193
6808
PRIMER_RIGHT_13_TM=57.121
6809
PRIMER_LEFT_13_GC_PERCENT=47.826
6810
PRIMER_RIGHT_13_GC_PERCENT=26.923
6811
PRIMER_LEFT_13_SELF_ANY=5.00
6812
PRIMER_RIGHT_13_SELF_ANY=6.00
6813
PRIMER_LEFT_13_SELF_END=0.00
6814
PRIMER_RIGHT_13_SELF_END=0.00
6815
PRIMER_LEFT_13_POSITION_PENALTY=0.000000
6816
PRIMER_RIGHT_13_POSITION_PENALTY=8.500000
6817
PRIMER_LEFT_13_END_STABILITY=9.4000
6818
PRIMER_RIGHT_13_END_STABILITY=6.7000
6819
PRIMER_PAIR_13_COMPL_ANY=5.00
6820
PRIMER_PAIR_13_COMPL_END=0.00
6821
PRIMER_PRODUCT_SIZE_13=3045
6822
PRIMER_PAIR_PENALTY_14=25.2441
6823
PRIMER_LEFT_14_PENALTY=7.193142
6824
PRIMER_RIGHT_14_PENALTY=18.051002
6825
PRIMER_LEFT_14_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGA
6826
PRIMER_RIGHT_14_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAAT
6827
PRIMER_LEFT_14=177,23
6828
PRIMER_RIGHT_14=3221,27
6829
PRIMER_LEFT_14_TM=64.193
6830
PRIMER_RIGHT_14_TM=57.449
6831
PRIMER_LEFT_14_GC_PERCENT=47.826
6832
PRIMER_RIGHT_14_GC_PERCENT=25.926
6833
PRIMER_LEFT_14_SELF_ANY=5.00
6834
PRIMER_RIGHT_14_SELF_ANY=6.00
6835
PRIMER_LEFT_14_SELF_END=0.00
6836
PRIMER_RIGHT_14_SELF_END=2.00
6837
PRIMER_LEFT_14_POSITION_PENALTY=0.000000
6838
PRIMER_RIGHT_14_POSITION_PENALTY=8.500000
6839
PRIMER_LEFT_14_END_STABILITY=9.4000
6840
PRIMER_RIGHT_14_END_STABILITY=6.9000
6841
PRIMER_PAIR_14_COMPL_ANY=5.00
6842
PRIMER_PAIR_14_COMPL_END=1.00
6843
PRIMER_PRODUCT_SIZE_14=3045
6844
PRIMER_PAIR_PENALTY_15=25.3529
6845
PRIMER_LEFT_15_PENALTY=7.193142
6846
PRIMER_RIGHT_15_PENALTY=18.159733
6847
PRIMER_LEFT_15_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGA
6848
PRIMER_RIGHT_15_SEQUENCE=ACTAAAATCCCACAAATTATAAGTCAA
6849
PRIMER_LEFT_15=177,23
6850
PRIMER_RIGHT_15=3222,27
6851
PRIMER_LEFT_15_TM=64.193
6852
PRIMER_RIGHT_15_TM=57.840
6853
PRIMER_LEFT_15_GC_PERCENT=47.826
6854
PRIMER_RIGHT_15_GC_PERCENT=25.926
6855
PRIMER_LEFT_15_SELF_ANY=5.00
6856
PRIMER_RIGHT_15_SELF_ANY=6.00
6857
PRIMER_LEFT_15_SELF_END=0.00
6858
PRIMER_RIGHT_15_SELF_END=0.00
6859
PRIMER_LEFT_15_POSITION_PENALTY=0.000000
6860
PRIMER_RIGHT_15_POSITION_PENALTY=9.000000
6861
PRIMER_LEFT_15_END_STABILITY=9.4000
6862
PRIMER_RIGHT_15_END_STABILITY=6.7000
6863
PRIMER_PAIR_15_COMPL_ANY=5.00
6864
PRIMER_PAIR_15_COMPL_END=0.00
6865
PRIMER_PRODUCT_SIZE_15=3046
6866
PRIMER_PAIR_PENALTY_16=25.6157
6867
PRIMER_LEFT_16_PENALTY=8.236744
6868
PRIMER_RIGHT_16_PENALTY=17.378993
6869
PRIMER_LEFT_16_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGAT
6870
PRIMER_RIGHT_16_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAA
6871
PRIMER_LEFT_16=177,24
6872
PRIMER_RIGHT_16=3221,26
6873
PRIMER_LEFT_16_TM=64.237
6874
PRIMER_RIGHT_16_TM=57.121
6875
PRIMER_LEFT_16_GC_PERCENT=45.833
6876
PRIMER_RIGHT_16_GC_PERCENT=26.923
6877
PRIMER_LEFT_16_SELF_ANY=5.00
6878
PRIMER_RIGHT_16_SELF_ANY=6.00
6879
PRIMER_LEFT_16_SELF_END=2.00
6880
PRIMER_RIGHT_16_SELF_END=0.00
6881
PRIMER_LEFT_16_POSITION_PENALTY=0.000000
6882
PRIMER_RIGHT_16_POSITION_PENALTY=8.500000
6883
PRIMER_LEFT_16_END_STABILITY=9.3000
6884
PRIMER_RIGHT_16_END_STABILITY=6.7000
6885
PRIMER_PAIR_16_COMPL_ANY=5.00
6886
PRIMER_PAIR_16_COMPL_END=1.00
6887
PRIMER_PRODUCT_SIZE_16=3045
6888
PRIMER_PAIR_PENALTY_17=25.6674
6889
PRIMER_LEFT_17_PENALTY=1.996678
6890
PRIMER_RIGHT_17_PENALTY=23.670687
6891
PRIMER_LEFT_17_SEQUENCE=ATGTCCTGGATGTTCAAGAGG
6892
PRIMER_RIGHT_17_SEQUENCE=TAGTCTTCTTACTAAAATCCCACAAAT
6893
PRIMER_LEFT_17=177,21
6894
PRIMER_RIGHT_17=3232,27
6895
PRIMER_LEFT_17_TM=59.003
6896
PRIMER_RIGHT_17_TM=57.329
6897
PRIMER_LEFT_17_GC_PERCENT=47.619
6898
PRIMER_RIGHT_17_GC_PERCENT=29.630
6899
PRIMER_LEFT_17_SELF_ANY=5.00
6900
PRIMER_RIGHT_17_SELF_ANY=5.00
6901
PRIMER_LEFT_17_SELF_END=0.00
6902
PRIMER_RIGHT_17_SELF_END=2.00
6903
PRIMER_LEFT_17_POSITION_PENALTY=0.000000
6904
PRIMER_RIGHT_17_POSITION_PENALTY=14.000000
6905
PRIMER_LEFT_17_END_STABILITY=7.9000
6906
PRIMER_RIGHT_17_END_STABILITY=7.2000
6907
PRIMER_PAIR_17_COMPL_ANY=5.00
6908
PRIMER_PAIR_17_COMPL_END=3.00
6909
PRIMER_PRODUCT_SIZE_17=3056
6910
PRIMER_PAIR_PENALTY_18=25.7689
6911
PRIMER_LEFT_18_PENALTY=1.996678
6912
PRIMER_RIGHT_18_PENALTY=23.772253
6913
PRIMER_LEFT_18_SEQUENCE=ATGTCCTGGATGTTCAAGAGG
6914
PRIMER_RIGHT_18_SEQUENCE=GTAGTCTTCTTACTAAAATCCCACAAA
6915
PRIMER_LEFT_18=177,21
6916
PRIMER_RIGHT_18=3233,27
6917
PRIMER_LEFT_18_TM=59.003
6918
PRIMER_RIGHT_18_TM=57.728
6919
PRIMER_LEFT_18_GC_PERCENT=47.619
6920
PRIMER_RIGHT_18_GC_PERCENT=33.333
6921
PRIMER_LEFT_18_SELF_ANY=5.00
6922
PRIMER_RIGHT_18_SELF_ANY=5.00
6923
PRIMER_LEFT_18_SELF_END=0.00
6924
PRIMER_RIGHT_18_SELF_END=0.00
6925
PRIMER_LEFT_18_POSITION_PENALTY=0.000000
6926
PRIMER_RIGHT_18_POSITION_PENALTY=14.500000
6927
PRIMER_LEFT_18_END_STABILITY=7.9000
6928
PRIMER_RIGHT_18_END_STABILITY=7.0000
6929
PRIMER_PAIR_18_COMPL_ANY=5.00
6930
PRIMER_PAIR_18_COMPL_END=2.00
6931
PRIMER_PRODUCT_SIZE_18=3057
6932
PRIMER_PAIR_PENALTY_19=26.2877
6933
PRIMER_LEFT_19_PENALTY=8.236744
6934
PRIMER_RIGHT_19_PENALTY=18.051002
6935
PRIMER_LEFT_19_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGAT
6936
PRIMER_RIGHT_19_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAAT
6937
PRIMER_LEFT_19=177,24
6938
PRIMER_RIGHT_19=3221,27
6939
PRIMER_LEFT_19_TM=64.237
6940
PRIMER_RIGHT_19_TM=57.449
6941
PRIMER_LEFT_19_GC_PERCENT=45.833
6942
PRIMER_RIGHT_19_GC_PERCENT=25.926
6943
PRIMER_LEFT_19_SELF_ANY=5.00
6944
PRIMER_RIGHT_19_SELF_ANY=6.00
6945
PRIMER_LEFT_19_SELF_END=2.00
6946
PRIMER_RIGHT_19_SELF_END=2.00
6947
PRIMER_LEFT_19_POSITION_PENALTY=0.000000
6948
PRIMER_RIGHT_19_POSITION_PENALTY=8.500000
6949
PRIMER_LEFT_19_END_STABILITY=9.3000
6950
PRIMER_RIGHT_19_END_STABILITY=6.9000
6951
PRIMER_PAIR_19_COMPL_ANY=5.00
6952
PRIMER_PAIR_19_COMPL_END=2.00
6953
PRIMER_PRODUCT_SIZE_19=3045
6954
=
Loggerhead is a web-based interface for Breezy
Version: 2.0.1