1
PRIMER PICKING RESULTS FOR pcr_primers
3
No mispriming library specified
4
Using 0-based sequence positions
5
OLIGO start len tm gc% any 3' seq
6
LEFT PRIMER 0 21 60.12 47.62 12.00 0.00 TCCTACAGCTGTGGGAAAATG
7
RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC
9
INCLUDED REGION SIZE: 161
11
PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
12
TARGETS (start, len)*: 21,122
14
0 TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagc
15
>>>>>>>>>>>>>>>>>>>>>***************************************
17
60 tatcatctactatcatctactatctactacacatctactatcatctacacacacacacac
18
************************************************************
20
120 actaggccgctatatcatctagcGCCGCCATGTACACACTG
21
***********************<<<<<<<<<<<<<<<<<<
23
KEYS (in order of precedence):
29
start len tm gc% any 3' seq
31
1 LEFT PRIMER 1 20 58.23 50.00 10.00 0.00 CCTACAGCTGTGGGAAAATG
32
RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC
33
PRODUCT SIZE: 160, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
35
2 LEFT PRIMER 0 20 57.27 45.00 12.00 2.00 TCCTACAGCTGTGGGAAAAT
36
RIGHT PRIMER 160 18 59.69 61.11 6.00 3.00 CAGTGTGTACATGGCGGC
37
PRODUCT SIZE: 161, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
40
con too in in no tm tm high high high
41
sid many tar excl bad GC too too any 3' poly end
42
ered Ns get reg GC% clamp low high compl compl X stab ok
43
Left 10 0 0 0 0 0 7 0 0 0 0 0 3
44
Right 1 0 0 0 0 0 0 0 0 0 0 0 1
50
PRIMER PICKING RESULTS FOR pcr_primers_and_io
52
No mispriming library specified
53
No internal oligo mishyb library specified
54
Using 0-based sequence positions
55
OLIGO start len tm gc% any 3' seq
56
LEFT PRIMER 9 22 59.90 40.91 6.00 4.00 TGTTTCACACTTACGGAAATGG
57
RIGHT PRIMER 247 20 60.23 50.00 3.00 0.00 TCCTGTTGGAGAAAAGCCAC
58
INTERNAL OLIGO 141 18 62.80 66.67 3.00 1.00 CTTAGCGTGCGTGGGGTC
60
INCLUDED REGION SIZE: 300
62
PRODUCT SIZE: 239, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
64
0 NATCCANGCTGTTTCACACTTACGGAAATGGANGACTNCCCATAGCTNCTGGGCTNTTAT
65
>>>>>>>>>>>>>>>>>>>>>>
67
60 CCAAAAAACATTCTCTNNNTGNTGCTTATTGGCNTCTCNAAGTGTGCNNACTTCATGGGC
70
120 CTACACANAATTGNNCANGGGCTTAGCGTGCGTGGGGTCCCTNGGTAACATNGGCATCTC
73
180 TGCACTTCNNGGTNNCCTTCCTTCTNACCAAGGGGTATCTGGNTGCCAGTGGCTTTTCTC
76
240 CAACAGGAANGGGGGTATGTGCATTNGCAGACATCAATGANCATGAGAGATATNAATAGG
79
KEYS (in order of precedence):
85
start len tm gc% any 3' seq
87
1 LEFT PRIMER 9 22 59.90 40.91 6.00 4.00 TGTTTCACACTTACGGAAATGG
88
RIGHT PRIMER 246 20 59.33 50.00 3.00 1.00 CCTGTTGGAGAAAAGCCACT
89
INTERNAL OLIGO 141 18 62.80 66.67 3.00 1.00 CTTAGCGTGCGTGGGGTC
90
PRODUCT SIZE: 238, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00
92
2 LEFT PRIMER 9 22 59.90 40.91 6.00 4.00 TGTTTCACACTTACGGAAATGG
93
RIGHT PRIMER 248 20 60.74 45.00 3.00 0.00 TTCCTGTTGGAGAAAAGCCA
94
INTERNAL OLIGO 141 18 62.80 66.67 3.00 1.00 CTTAGCGTGCGTGGGGTC
95
PRODUCT SIZE: 240, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00
97
3 LEFT PRIMER 11 21 58.13 38.10 6.00 6.00 TTTCACACTTACGGAAATGGA
98
RIGHT PRIMER 247 20 60.23 50.00 3.00 0.00 TCCTGTTGGAGAAAAGCCAC
99
INTERNAL OLIGO 141 18 62.80 66.67 3.00 1.00 CTTAGCGTGCGTGGGGTC
100
PRODUCT SIZE: 237, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
102
4 LEFT PRIMER 10 22 59.00 40.91 6.00 6.00 GTTTCACACTTACGGAAATGGA
103
RIGHT PRIMER 247 20 60.23 50.00 3.00 0.00 TCCTGTTGGAGAAAAGCCAC
104
INTERNAL OLIGO 141 18 62.80 66.67 3.00 1.00 CTTAGCGTGCGTGGGGTC
105
PRODUCT SIZE: 238, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
108
con too in in no tm tm high high high
109
sid many tar excl bad GC too too any 3' poly end
110
ered Ns get reg GC% clamp low high compl compl X stab ok
111
Left 281 210 0 0 0 0 30 29 0 0 0 0 12
112
Right 284 210 0 0 0 0 7 44 0 0 0 0 23
113
Intl 393 280 0 0 0 0 30 46 0 0 3 0 34
119
PRIMER PICKING RESULTS FOR left_only
121
No mispriming library specified
122
Using 0-based sequence positions
123
OLIGO start len tm gc% any 3' seq
124
LEFT_PRIMER 72 20 59.96 55.00 3.00 0.00 TTAGAAGGGGTGTCTGGGTG
126
INCLUDED REGION SIZE: 175
128
TARGETS (start, len)*: 100,20
129
0 GNATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCA
132
60 CACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGG
133
>>>>>>>>>>>>>>>>>>>> ********************
135
120 GACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC
138
KEYS (in order of precedence):
144
start len tm gc% any 3' seq
145
1 LEFT_PRIMER 64 20 59.73 55.00 6.00 0.00 CTGCAGGTTTAGAAGGGGTG
146
2 LEFT_PRIMER 60 20 59.73 55.00 8.00 0.00 CACCCTGCAGGTTTAGAAGG
147
3 LEFT_PRIMER 56 20 60.31 55.00 8.00 6.00 AGCACACCCTGCAGGTTTAG
148
4 LEFT_PRIMER 65 20 59.59 50.00 4.00 0.00 TGCAGGTTTAGAAGGGGTGT
151
con too in in no tm tm high high high
152
sid many tar excl bad GC too too any 3' poly end
153
ered Ns get reg GC% clamp low high compl compl X stab ok
154
Left 1121 38 23 0 0 0 42 866 0 0 0 0 152
158
PRIMER PICKING RESULTS FOR right_only
160
No mispriming library specified
161
Using 0-based sequence positions
162
OLIGO start len tm gc% any 3' seq
163
RIGHT_PRIMER 125 20 60.29 45.00 3.00 1.00 GTGCTTTGCGTTTTGGAAGT
165
INCLUDED REGION SIZE: 136
167
TARGETS (start, len)*: 90,10
168
0 GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGAC
171
60 CTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCA
172
********** <<<<<<<<<<<<<<
177
KEYS (in order of precedence):
183
start len tm gc% any 3' seq
184
1 RIGHT_PRIMER 75 20 59.27 45.00 2.00 1.00 TTGGCGTTTCTATCAGGTCA
185
2 RIGHT_PRIMER 124 20 60.78 40.00 3.00 3.00 TGCTTTGCGTTTTGGAAGTT
186
3 RIGHT_PRIMER 76 21 60.13 47.62 2.00 1.00 GTTGGCGTTTCTATCAGGTCA
187
4 RIGHT_PRIMER 79 21 60.26 47.62 3.00 0.00 TGAGTTGGCGTTTCTATCAGG
190
con too in in no tm tm high high high
191
sid many tar excl bad GC too too any 3' poly end
192
ered Ns get reg GC% clamp low high compl compl X stab ok
193
Right 623 27 36 0 5 0 230 145 0 6 0 0 174
197
PRIMER PICKING RESULTS FOR hyb_probe_only
199
Using 0-based sequence positions
200
OLIGO start len tm gc% any 3' seq
201
INTERNAL_OLIGO 64 20 59.73 55.00 6.00 0.00 CTGCAGGTTTAGAAGGGGTG
203
INCLUDED REGION SIZE: 175
205
INTERNAL OLIGO EXCLUDED REGIONS (start, len)*: 90,20
206
0 GCATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCA
209
60 CACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGG
210
^^^^^^^^^^^^^^^^^^^^ xxxxxxxxxxxxxxxxxxxx
212
120 GACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC
215
KEYS (in order of precedence):
216
^^^^^^ internal oligo
220
start len tm gc% any 3' seq
221
1 INTERNAL_OLIGO 60 20 59.73 55.00 8.00 0.00 CACCCTGCAGGTTTAGAAGG
222
2 INTERNAL_OLIGO 56 20 60.31 55.00 8.00 6.00 AGCACACCCTGCAGGTTTAG
223
3 INTERNAL_OLIGO 1 20 60.38 55.00 4.00 0.00 CATCTGCACCCTGTAAACCC
224
4 INTERNAL_OLIGO 65 20 59.59 50.00 4.00 0.00 TGCAGGTTTAGAAGGGGTGT
227
con too in in no tm tm high high high
228
sid many tar excl bad GC too too any 3' poly end
229
ered Ns get reg GC% clamp low high compl compl X stab ok
230
Intl 1040 27 0 33 0 0 44 804 0 0 0 0 132
234
PRIMER PICKING RESULTS FOR left_only
236
No mispriming library specified
237
Using 0-based sequence positions
238
OLIGO start len tm gc% any 3' seq
239
LEFT_PRIMER 0 20 59.96 55.00 3.00 0.00 TTAGAAGGGGTGTCTGGGTG
241
INCLUDED REGION SIZE: 20
243
0 TTAGAAGGGGTGTCTGGGTG
246
KEYS (in order of precedence):
252
con too in in no tm tm high high high
253
sid many tar excl bad GC too too any 3' poly end
254
ered Ns get reg GC% clamp low high compl compl X stab ok
255
Left 1 0 0 0 0 0 0 0 0 0 0 0 1
259
PRIMER PICKING RESULTS FOR right_only
261
No mispriming library specified
262
Using 0-based sequence positions
263
OLIGO start len tm gc% any 3' seq
264
LEFT_PRIMER 0 20 59.96 55.00 3.00 0.00 TTAGAAGGGGTGTCTGGGTG
266
INCLUDED REGION SIZE: 20
268
0 TTAGAAGGGGTGTCTGGGTG
271
KEYS (in order of precedence):
277
con too in in no tm tm high high high
278
sid many tar excl bad GC too too any 3' poly end
279
ered Ns get reg GC% clamp low high compl compl X stab ok
280
Left 1 0 0 0 0 0 0 0 0 0 0 0 1
284
PRIMER PICKING RESULTS FOR hyb_probe_only
286
No mispriming library specified
287
Using 0-based sequence positions
288
OLIGO start len tm gc% any 3' seq
289
LEFT_PRIMER 0 20 59.96 55.00 3.00 0.00 TTAGAAGGGGTGTCTGGGTG
291
INCLUDED REGION SIZE: 20
293
0 TTAGAAGGGGTGTCTGGGTG
296
KEYS (in order of precedence):
302
con too in in no tm tm high high high
303
sid many tar excl bad GC too too any 3' poly end
304
ered Ns get reg GC% clamp low high compl compl X stab ok
305
Left 1 0 0 0 0 0 0 0 0 0 0 0 1