1
ACCEPTABLE RIGHT PRIMERS
2
1-based # self self qual-
3
# sequence start ln N GC% Tm any end lity
4
0 CACAATACCACACATGTTTAACAGACA 120 27 0 37.04 62.089 7.00 3.00 9.089
5
1 CACAATACCACACATGTTTAACAGAC 120 26 0 38.46 59.680 7.00 1.00 6.320
6
2 GCACAATACCACACATGTTTAACAGAC 121 27 0 40.74 62.806 7.00 1.00 9.806
7
3 CACAATACCACACATGTTTAACAGA 120 25 0 36.00 58.912 7.00 3.00 6.088
8
4 GCACAATACCACACATGTTTAACAGA 121 26 0 38.46 62.185 7.00 3.00 8.185
9
5 CACAATACCACACATGTTTAACAG 120 24 0 37.50 57.140 7.00 5.00 6.860
10
6 GCACAATACCACACATGTTTAACAG 121 25 0 40.00 60.599 7.00 5.00 5.599
11
7 GCACAATACCACACATGTTTAACA 121 24 0 37.50 59.733 7.00 7.00 4.267
12
8 TGCACAATACCACACATGTTTAACA 122 25 0 36.00 62.264 7.00 7.00 7.264
13
9 ATGCACAATACCACACATGTTTAACA 123 26 0 34.62 62.397 7.00 7.00 8.397
14
10 TATGCACAATACCACACATGTTTAACA 124 27 0 33.33 62.329 7.00 7.00 9.329
15
11 GCACAATACCACACATGTTTAAC 121 23 0 39.13 57.060 6.00 5.00 5.940
16
12 TGCACAATACCACACATGTTTAAC 122 24 0 37.50 59.733 6.00 5.00 4.267
17
13 ATGCACAATACCACACATGTTTAAC 123 25 0 36.00 59.991 6.00 5.00 5.009
18
14 TATGCACAATACCACACATGTTTAAC 124 26 0 34.62 60.001 6.00 5.00 6.001
19
15 ATATGCACAATACCACACATGTTTAAC 125 27 0 33.33 60.227 6.00 5.00 7.227
20
16 TGCACAATACCACACATGTTTAA 122 23 0 34.78 58.925 6.00 4.00 4.075
21
17 ATGCACAATACCACACATGTTTAA 123 24 0 33.33 59.233 6.00 4.00 4.767
22
18 TATGCACAATACCACACATGTTTAA 124 25 0 32.00 59.268 6.00 4.00 5.732
23
19 ATATGCACAATACCACACATGTTTAA 125 26 0 30.77 59.536 6.00 4.00 6.464
24
20 TATATGCACAATACCACACATGTTTAA 126 27 0 29.63 59.560 6.00 4.00 7.440
25
21 TGCACAATACCACACATGTTTA 122 22 0 36.36 57.504 6.00 4.00 4.496
26
22 ATGCACAATACCACACATGTTTA 123 23 0 34.78 57.903 6.00 4.00 5.097
27
23 TATGCACAATACCACACATGTTTA 124 24 0 33.33 57.988 6.00 4.00 6.012
28
24 ATATGCACAATACCACACATGTTTA 125 25 0 32.00 58.331 6.00 4.00 6.669
29
25 TATATGCACAATACCACACATGTTTA 126 26 0 30.77 58.395 6.00 4.00 7.605
30
26 GTATATGCACAATACCACACATGTTTA 127 27 0 33.33 59.133 6.00 4.00 7.867
31
27 TGCACAATACCACACATGTTT 122 21 0 38.10 57.391 6.00 6.00 3.609
32
28 ATGCACAATACCACACATGTTT 123 22 0 36.36 57.811 6.00 6.00 4.189
33
29 TATGCACAATACCACACATGTTT 124 23 0 34.78 57.903 6.00 6.00 5.097
34
30 ATATGCACAATACCACACATGTTT 125 24 0 33.33 58.262 6.00 6.00 5.738
35
31 TATATGCACAATACCACACATGTTT 126 25 0 32.00 58.331 6.00 6.00 6.669
36
32 GTATATGCACAATACCACACATGTTT 127 26 0 34.62 59.095 6.00 6.00 6.905
37
33 TGTATATGCACAATACCACACATGTTT 128 27 0 33.33 61.445 6.00 6.00 8.445
38
34 TATATGCACAATACCACACATGTT 126 24 0 33.33 57.002 6.00 4.00 6.998
39
35 GTATATGCACAATACCACACATGTT 127 25 0 36.00 57.851 6.00 4.00 7.149
40
36 TGTATATGCACAATACCACACATGTT 128 26 0 34.62 60.346 6.00 4.00 6.346
41
37 ATGTATATGCACAATACCACACATGTT 129 27 0 33.33 60.562 6.00 4.00 7.562
42
38 TGTATATGCACAATACCACACATGT 128 25 0 36.00 59.136 6.00 6.00 5.864
43
39 ATGTATATGCACAATACCACACATGT 129 26 0 34.62 59.420 6.00 6.00 6.580
44
40 CATGTATATGCACAATACCACACATGT 130 27 0 37.04 61.814 6.00 6.00 8.814
45
41 TGTATATGCACAATACCACACATG 128 24 0 37.50 58.315 6.00 4.00 5.685
46
42 ATGTATATGCACAATACCACACATG 129 25 0 36.00 58.650 6.00 4.00 6.350
47
43 CATGTATATGCACAATACCACACATG 130 26 0 38.46 61.147 6.00 4.00 7.147
48
44 ACATGTATATGCACAATACCACACATG 131 27 0 37.04 61.814 6.00 4.00 8.814
49
45 CATGTATATGCACAATACCACACAT 130 25 0 36.00 58.650 6.00 2.00 6.350
50
46 ACATGTATATGCACAATACCACACAT 131 26 0 34.62 59.420 6.00 2.00 6.580
51
47 CACATGTATATGCACAATACCACACAT 132 27 0 37.04 61.814 6.00 2.00 8.814
52
48 CATGTATATGCACAATACCACACA 130 24 0 37.50 58.315 6.00 0.00 5.685
53
49 ACATGTATATGCACAATACCACACA 131 25 0 36.00 59.136 6.00 0.00 5.864
54
50 CACATGTATATGCACAATACCACACA 132 26 0 38.46 61.654 6.00 0.00 7.654
55
51 ACACATGTATATGCACAATACCACACA 133 27 0 37.04 62.312 6.00 0.00 9.312
56
52 CACATGTATATGCACAATACCACAC 132 25 0 40.00 59.136 6.00 0.00 5.864
57
53 ACACATGTATATGCACAATACCACAC 133 26 0 38.46 59.900 6.00 0.00 6.100
58
54 CACACATGTATATGCACAATACCACAC 134 27 0 40.74 62.312 6.00 0.00 9.312
59
55 CACATGTATATGCACAATACCACA 132 24 0 37.50 58.315 6.00 2.00 5.685
60
56 ACACATGTATATGCACAATACCACA 133 25 0 36.00 59.136 6.00 2.00 5.864
61
57 CACACATGTATATGCACAATACCACA 134 26 0 38.46 61.654 6.00 2.00 7.654
62
58 ACACACATGTATATGCACAATACCACA 135 27 0 37.04 62.312 6.00 2.00 9.312
63
59 CACACATGTATATGCACAATACCAC 134 25 0 40.00 59.136 6.00 2.00 5.864
64
60 ACACACATGTATATGCACAATACCAC 135 26 0 38.46 59.900 6.00 2.00 6.100
65
61 AACACACATGTATATGCACAATACCAC 136 27 0 37.04 61.034 6.00 2.00 8.034
66
62 CACACATGTATATGCACAATACCA 134 24 0 37.50 58.315 6.00 4.00 5.685
67
63 ACACACATGTATATGCACAATACCA 135 25 0 36.00 59.136 6.00 4.00 5.864
68
64 AACACACATGTATATGCACAATACCA 136 26 0 34.62 60.346 6.00 4.00 6.346
69
65 CAACACACATGTATATGCACAATACCA 137 27 0 37.04 62.716 6.00 4.00 9.716
70
66 AACACACATGTATATGCACAATACC 136 25 0 36.00 57.851 6.00 4.00 7.149
71
67 CAACACACATGTATATGCACAATACC 137 26 0 38.46 60.346 6.00 4.00 6.346
72
68 ACAACACACATGTATATGCACAATACC 138 27 0 37.04 61.034 6.00 4.00 8.034
73
69 CAACACACATGTATATGCACAATAC 137 25 0 36.00 57.073 6.00 6.00 7.927
74
70 ACAACACACATGTATATGCACAATAC 138 26 0 34.62 57.913 6.00 6.00 8.087
75
71 CACAACACACATGTATATGCACAATAC 139 27 0 37.04 60.389 6.00 6.00 7.389
76
72 ACAACACACATGTATATGCACAATA 138 25 0 32.00 57.073 6.00 4.00 7.927
77
73 CACAACACACATGTATATGCACAATA 139 26 0 34.62 59.659 6.00 4.00 6.341
78
74 ACACAACACACATGTATATGCACAATA 140 27 0 33.33 60.389 6.00 4.00 7.389
79
75 CACAACACACATGTATATGCACAAT 139 25 0 36.00 59.637 6.00 2.00 5.363
80
76 ACACAACACACATGTATATGCACAAT 140 26 0 34.62 60.393 6.00 2.00 6.393
81
77 TACACAACACACATGTATATGCACAAT 141 27 0 33.33 60.389 6.00 2.00 7.389
82
78 CACAACACACATGTATATGCACAA 139 24 0 37.50 59.345 6.00 2.00 4.655
83
79 ACACAACACACATGTATATGCACAA 140 25 0 36.00 60.151 6.00 2.00 5.151
84
80 TACACAACACACATGTATATGCACAA 141 26 0 34.62 60.156 6.00 2.00 6.156
85
81 ATACACAACACACATGTATATGCACAA 142 27 0 33.33 60.389 6.00 2.00 7.389
86
82 CACAACACACATGTATATGCACA 139 23 0 39.13 57.923 6.00 4.00 5.077
87
83 ACACAACACACATGTATATGCACA 140 24 0 37.50 58.828 6.00 4.00 5.172
88
84 TACACAACACACATGTATATGCACA 141 25 0 36.00 58.882 6.00 4.00 6.118
89
85 ATACACAACACACATGTATATGCACA 142 26 0 34.62 59.190 6.00 4.00 6.810
90
86 CATACACAACACACATGTATATGCACA 143 27 0 37.04 61.688 6.00 4.00 8.688
91
87 CATACACAACACACATGTATATGCAC 143 26 0 38.46 59.190 6.00 4.00 6.810
92
88 CCATACACAACACACATGTATATGCAC 144 27 0 40.74 62.312 6.00 4.00 9.312
93
89 CATACACAACACACATGTATATGCA 143 25 0 36.00 58.378 6.00 6.00 6.622
94
90 CCATACACAACACACATGTATATGCA 144 26 0 38.46 61.654 6.00 6.00 7.654
95
91 CCATACACAACACACATGTATATGC 144 25 0 40.00 59.136 6.00 4.00 5.864
96
92 TCCATACACAACACACATGTATATGC 145 26 0 38.46 60.811 6.00 4.00 6.811
97
93 ATCCATACACAACACACATGTATATGC 146 27 0 37.04 61.010 6.00 4.00 8.010
98
94 TCCATACACAACACACATGTATATG 145 25 0 36.00 57.352 6.00 6.00 7.648
99
95 ATCCATACACAACACACATGTATATG 146 26 0 34.62 57.730 6.00 6.00 8.270
100
96 CATCCATACACAACACACATGTATATG 147 27 0 37.04 60.230 6.00 6.00 7.230
101
97 CATCCATACACAACACACATGTATAT 147 26 0 34.62 57.730 6.00 6.00 8.270
102
98 ACATCCATACACAACACACATGTATAT 148 27 0 33.33 58.526 6.00 6.00 8.474
103
99 CATCCATACACAACACACATGTATA 147 25 0 36.00 57.352 6.00 4.00 7.648
104
100 ACATCCATACACAACACACATGTATA 148 26 0 34.62 58.200 6.00 4.00 7.800
105
101 TACATCCATACACAACACACATGTATA 149 27 0 33.33 58.271 6.00 4.00 8.729
106
102 CATCCATACACAACACACATGTAT 147 24 0 37.50 57.241 6.00 6.00 6.759
107
103 ACATCCATACACAACACACATGTAT 148 25 0 36.00 58.124 6.00 6.00 6.876
108
104 TACATCCATACACAACACACATGTAT 149 26 0 34.62 58.200 6.00 6.00 7.800
109
105 CTACATCCATACACAACACACATGTAT 150 27 0 37.04 59.113 6.00 6.00 7.887
110
106 ACATCCATACACAACACACATGTA 148 24 0 37.50 57.747 6.00 4.00 6.253
111
107 TACATCCATACACAACACACATGTA 149 25 0 36.00 57.841 6.00 4.00 7.159
112
108 CTACATCCATACACAACACACATGTA 150 26 0 38.46 58.815 6.00 4.00 7.185
113
109 GCTACATCCATACACAACACACATGTA 151 27 0 40.74 62.100 6.00 4.00 9.100
114
110 ACATCCATACACAACACACATGT 148 23 0 39.13 57.646 6.00 6.00 5.354
115
111 TACATCCATACACAACACACATGT 149 24 0 37.50 57.747 6.00 6.00 6.253
116
112 CTACATCCATACACAACACACATGT 150 25 0 40.00 58.761 6.00 6.00 6.239
117
113 GCTACATCCATACACAACACACATGT 151 26 0 42.31 62.163 6.00 6.00 8.163
118
114 AGCTACATCCATACACAACACACATGT 152 27 0 40.74 62.902 6.00 6.00 9.902
119
115 CTACATCCATACACAACACACATG 150 24 0 41.67 57.896 4.00 4.00 6.104
120
116 GCTACATCCATACACAACACACATG 151 25 0 44.00 61.472 4.00 4.00 6.472
121
117 AGCTACATCCATACACAACACACATG 152 26 0 42.31 62.266 4.00 4.00 8.266
122
118 GCTACATCCATACACAACACACAT 151 24 0 41.67 58.842 2.00 2.00 5.158
123
119 AGCTACATCCATACACAACACACAT 152 25 0 40.00 59.772 4.00 2.00 5.228
124
120 AAGCTACATCCATACACAACACACAT 153 26 0 38.46 60.948 4.00 2.00 6.948
125
121 GAAGCTACATCCATACACAACACACAT 154 27 0 40.74 62.494 4.00 2.00 9.494
126
122 GCTACATCCATACACAACACACA 151 23 0 43.48 58.499 2.00 0.00 4.501
127
123 AGCTACATCCATACACAACACACA 152 24 0 41.67 59.493 4.00 0.00 4.507
128
124 AAGCTACATCCATACACAACACACA 153 25 0 40.00 60.742 4.00 0.00 5.742
129
125 GAAGCTACATCCATACACAACACACA 154 26 0 42.31 62.368 4.00 0.00 8.368
130
126 AAGCTACATCCATACACAACACAC 153 24 0 41.67 58.137 4.00 0.00 5.863
131
127 GAAGCTACATCCATACACAACACAC 154 25 0 44.00 59.880 4.00 0.00 5.120
132
128 AGAAGCTACATCCATACACAACACAC 155 26 0 42.31 60.727 4.00 0.00 6.727
133
129 AAGAAGCTACATCCATACACAACACAC 156 27 0 40.74 61.811 4.00 0.00 8.811
134
130 AAGCTACATCCATACACAACACA 153 23 0 39.13 57.257 4.00 0.00 5.743
135
131 GAAGCTACATCCATACACAACACA 154 24 0 41.67 59.091 4.00 0.00 4.909
136
132 AGAAGCTACATCCATACACAACACA 155 25 0 40.00 60.002 4.00 0.00 5.002
137
133 AAGAAGCTACATCCATACACAACACA 156 26 0 38.46 61.157 4.00 0.00 7.157
138
134 AGAAGCTACATCCATACACAACAC 155 24 0 41.67 57.400 4.00 0.00 6.600
139
135 AAGAAGCTACATCCATACACAACAC 156 25 0 40.00 58.712 4.00 0.00 6.288
140
136 CAAGAAGCTACATCCATACACAACAC 157 26 0 42.31 61.157 4.00 0.00 7.157
141
137 TCAAGAAGCTACATCCATACACAACAC 158 27 0 40.74 62.685 4.00 0.00 9.685
142
138 AAGAAGCTACATCCATACACAACA 156 24 0 37.50 57.904 4.00 0.00 6.096
143
139 CAAGAAGCTACATCCATACACAACA 157 25 0 40.00 60.458 4.00 0.00 5.458
144
140 TCAAGAAGCTACATCCATACACAACA 158 26 0 38.46 62.056 4.00 0.00 8.056
145
141 GTCAAGAAGCTACATCCATACACAACA 159 27 0 40.74 62.685 4.00 0.00 9.685
146
142 CAAGAAGCTACATCCATACACAAC 157 24 0 41.67 57.904 4.00 0.00 6.096
147
143 TCAAGAAGCTACATCCATACACAAC 158 25 0 40.00 59.615 4.00 0.00 5.385
148
144 GTCAAGAAGCTACATCCATACACAAC 159 26 0 42.31 60.343 4.00 0.00 6.343
149
145 AGTCAAGAAGCTACATCCATACACAAC 160 27 0 40.74 61.126 4.00 0.00 8.126
150
146 CAAGAAGCTACATCCATACACAA 157 23 0 39.13 57.036 4.00 0.00 5.964
151
147 TCAAGAAGCTACATCCATACACAA 158 24 0 37.50 58.834 4.00 0.00 5.166
152
148 GTCAAGAAGCTACATCCATACACAA 159 25 0 40.00 59.615 4.00 0.00 5.385
153
149 AGTCAAGAAGCTACATCCATACACAA 160 26 0 38.46 60.455 4.00 0.00 6.455
154
150 CAGTCAAGAAGCTACATCCATACACAA 161 27 0 40.74 62.778 4.00 0.00 9.778
155
151 TCAAGAAGCTACATCCATACACA 158 23 0 39.13 57.467 4.00 0.00 5.533
156
152 GTCAAGAAGCTACATCCATACACA 159 24 0 41.67 58.339 4.00 0.00 5.661
157
153 AGTCAAGAAGCTACATCCATACACA 160 25 0 40.00 59.274 4.00 0.00 5.726
158
154 CAGTCAAGAAGCTACATCCATACACA 161 26 0 42.31 61.740 4.00 0.00 7.740
159
155 ACAGTCAAGAAGCTACATCCATACACA 162 27 0 40.74 62.383 4.00 0.00 9.383
160
156 CAGTCAAGAAGCTACATCCATACAC 161 25 0 44.00 59.274 4.00 0.00 5.726
161
157 ACAGTCAAGAAGCTACATCCATACAC 162 26 0 42.31 60.018 4.00 0.00 6.018
162
158 CACAGTCAAGAAGCTACATCCATACAC 163 27 0 44.44 62.383 4.00 0.00 9.383
163
159 CAGTCAAGAAGCTACATCCATACA 161 24 0 41.67 58.476 4.00 0.00 5.524
164
160 ACAGTCAAGAAGCTACATCCATACA 162 25 0 40.00 59.274 4.00 0.00 5.726
165
161 CACAGTCAAGAAGCTACATCCATACA 163 26 0 42.31 61.740 4.00 0.00 7.740
166
162 ACACAGTCAAGAAGCTACATCCATACA 164 27 0 40.74 62.383 4.00 0.00 9.383
167
163 CACAGTCAAGAAGCTACATCCATAC 163 25 0 44.00 59.274 4.00 0.00 5.726
168
164 ACACAGTCAAGAAGCTACATCCATAC 164 26 0 42.31 60.018 4.00 0.00 6.018
169
165 CACACAGTCAAGAAGCTACATCCATAC 165 27 0 44.44 62.383 4.00 0.00 9.383
170
166 CACAGTCAAGAAGCTACATCCATA 163 24 0 41.67 58.476 4.00 2.00 5.524
171
167 ACACAGTCAAGAAGCTACATCCATA 164 25 0 40.00 59.274 4.00 2.00 5.726
172
168 CACACAGTCAAGAAGCTACATCCATA 165 26 0 42.31 61.740 4.00 2.00 7.740
173
169 ACACACAGTCAAGAAGCTACATCCATA 166 27 0 40.74 62.383 4.00 2.00 9.383
174
170 CACAGTCAAGAAGCTACATCCAT 163 23 0 43.48 58.408 4.00 2.00 4.592
175
171 ACACAGTCAAGAAGCTACATCCAT 164 24 0 41.67 59.238 4.00 2.00 4.762
176
172 CACACAGTCAAGAAGCTACATCCAT 165 25 0 44.00 61.791 4.00 2.00 6.791
177
173 ACACACAGTCAAGAAGCTACATCCAT 166 26 0 42.31 62.454 4.00 2.00 8.454
178
174 CACAGTCAAGAAGCTACATCCA 163 22 0 45.45 58.036 4.00 0.00 3.964
179
175 ACACAGTCAAGAAGCTACATCCA 164 23 0 43.48 58.925 4.00 0.00 4.075
180
176 CACACAGTCAAGAAGCTACATCCA 165 24 0 45.83 61.619 4.00 0.00 5.619
181
177 ACACACAGTCAAGAAGCTACATCCA 166 25 0 44.00 62.322 4.00 0.00 7.322
182
178 CACACAGTCAAGAAGCTACATCC 165 23 0 47.83 58.925 4.00 0.00 4.075
183
179 ACACACAGTCAAGAAGCTACATCC 166 24 0 45.83 59.748 4.00 0.00 4.252
184
180 AACACACAGTCAAGAAGCTACATCC 167 25 0 44.00 60.962 4.00 0.00 5.962
185
181 AACACACAGTCAAGAAGCTACATC 167 24 0 41.67 57.626 4.00 2.00 6.374
186
182 CAACACACAGTCAAGAAGCTACATC 168 25 0 44.00 60.272 4.00 2.00 5.272
187
183 ACAACACACAGTCAAGAAGCTACATC 169 26 0 42.31 61.001 4.00 2.00 7.001
188
184 CAACACACAGTCAAGAAGCTACAT 168 24 0 41.67 58.520 4.00 4.00 5.480
189
185 ACAACACACAGTCAAGAAGCTACAT 169 25 0 40.00 59.333 4.00 4.00 5.667
190
186 CACAACACACAGTCAAGAAGCTACAT 170 26 0 42.31 61.844 4.00 4.00 7.844
191
187 ACACAACACACAGTCAAGAAGCTACAT 171 27 0 40.74 62.495 4.00 4.00 9.495
192
188 CAACACACAGTCAAGAAGCTACA 168 23 0 43.48 58.160 4.00 2.00 4.840
193
189 ACAACACACAGTCAAGAAGCTACA 169 24 0 41.67 59.030 4.00 2.00 4.970
194
190 CACAACACACAGTCAAGAAGCTACA 170 25 0 44.00 61.678 4.00 2.00 6.678
195
191 ACACAACACACAGTCAAGAAGCTACA 171 26 0 42.31 62.367 4.00 2.00 8.367
196
192 CACAACACACAGTCAAGAAGCTAC 170 24 0 45.83 59.030 4.00 4.00 4.970
197
193 ACACAACACACAGTCAAGAAGCTAC 171 25 0 44.00 59.835 4.00 4.00 5.165
198
194 CACACAACACACAGTCAAGAAGCTAC 172 26 0 46.15 62.367 4.00 4.00 8.367
199
195 CACAACACACAGTCAAGAAGCTA 170 23 0 43.48 58.160 4.00 2.00 4.840
200
196 ACACAACACACAGTCAAGAAGCTA 171 24 0 41.67 59.030 4.00 2.00 4.970
201
197 CACACAACACACAGTCAAGAAGCTA 172 25 0 44.00 61.678 4.00 2.00 6.678
202
198 ACACACAACACACAGTCAAGAAGCTA 173 26 0 42.31 62.367 4.00 2.00 8.367
203
199 CACAACACACAGTCAAGAAGCT 170 22 0 45.45 58.075 4.00 4.00 3.925
204
200 ACACAACACACAGTCAAGAAGCT 171 23 0 43.48 58.981 4.00 4.00 4.019
205
201 CACACAACACACAGTCAAGAAGCT 172 24 0 45.83 61.730 4.00 4.00 5.730
206
202 ACACACAACACACAGTCAAGAAGCT 173 25 0 44.00 62.443 4.00 4.00 7.443
207
203 ACACAACACACAGTCAAGAAGC 171 22 0 45.45 57.918 3.00 2.00 4.082
208
204 CACACAACACACAGTCAAGAAGC 172 23 0 47.83 60.832 3.00 2.00 3.832
209
205 ACACACAACACACAGTCAAGAAGC 173 24 0 45.83 61.613 3.00 2.00 5.613
210
206 ACACACAACACACAGTCAAGAAG 173 23 0 43.48 57.837 3.00 0.00 5.163
211
207 CACACACAACACACAGTCAAGAAG 174 24 0 45.83 60.730 3.00 0.00 4.730
212
208 ACACACACAACACACAGTCAAGAAG 175 25 0 44.00 61.508 3.00 0.00 6.508
213
209 CACACACAACACACAGTCAAGAA 174 23 0 43.48 59.744 3.00 0.00 3.256
214
210 ACACACACAACACACAGTCAAGAA 175 24 0 41.67 60.599 3.00 0.00 4.599
215
211 CACACACAACACACAGTCAAGA 174 22 0 45.45 58.217 3.00 2.00 3.783
216
212 ACACACACAACACACAGTCAAGA 175 23 0 43.48 59.188 3.00 2.00 3.812
217
213 CACACACACAACACACAGTCAAGA 176 24 0 45.83 62.141 3.00 2.00 6.141
218
214 ACACACACACAACACACAGTCAAGA 177 25 0 44.00 62.889 3.00 2.00 7.889
219
215 ACACACACAACACACAGTCAAG 175 22 0 45.45 57.090 3.00 0.00 4.910
220
216 CACACACACAACACACAGTCAAG 176 23 0 47.83 60.208 3.00 0.00 3.208
221
217 ACACACACACAACACACAGTCAAG 177 24 0 45.83 61.059 3.00 0.00 5.059
222
218 CACACACACAACACACAGTCAA 176 22 0 45.45 59.121 3.00 0.00 2.879
223
219 ACACACACACAACACACAGTCAA 177 23 0 43.48 60.064 3.00 0.00 3.064
224
220 CACACACACAACACACAGTCA 176 21 0 47.62 57.434 3.00 1.00 3.566
225
221 ACACACACACAACACACAGTCA 177 22 0 45.45 58.514 3.00 1.00 3.486
226
222 CACACACACACAACACACAGTCA 178 23 0 47.83 61.701 3.00 1.00 4.701
227
223 ACACACACACACAACACACAGTCA 179 24 0 45.83 62.520 3.00 1.00 6.520
228
224 CACACACACACAACACACAGTC 178 22 0 50.00 58.514 3.00 1.00 3.486
229
225 ACACACACACACAACACACAGTC 179 23 0 47.83 59.499 3.00 1.00 3.501
230
226 GACACACACACACAACACACAGTC 180 24 0 50.00 61.504 3.00 1.00 5.504
231
227 AGACACACACACACAACACACAGTC 181 25 0 48.00 62.414 3.00 1.00 7.414
232
228 ACACACACACACAACACACAGT 179 22 0 45.45 57.352 3.00 3.00 4.648
233
229 GACACACACACACAACACACAGT 180 23 0 47.83 59.499 3.00 3.00 3.501
234
230 AGACACACACACACAACACACAGT 181 24 0 45.83 60.539 3.00 3.00 4.539
235
231 GACACACACACACAACACACAG 180 22 0 50.00 58.514 1.00 1.00 3.486
236
232 AGACACACACACACAACACACAG 181 23 0 47.83 59.650 1.00 1.00 3.350
237
233 CAGACACACACACACAACACACAG 182 24 0 50.00 62.641 1.00 1.00 6.641
238
234 GACACACACACACAACACACA 180 21 0 47.62 57.244 1.00 0.00 3.756
239
235 AGACACACACACACAACACACA 181 22 0 45.45 58.514 1.00 0.00 3.486
240
236 CAGACACACACACACAACACACA 182 23 0 47.83 61.701 1.00 0.00 4.701
241
237 ACAGACACACACACACAACACACA 183 24 0 45.83 62.520 1.00 0.00 6.520
242
238 CAGACACACACACACAACACAC 182 22 0 50.00 58.514 1.00 0.00 3.486
243
239 ACAGACACACACACACAACACAC 183 23 0 47.83 59.499 1.00 0.00 3.501
244
240 AACAGACACACACACACAACACAC 184 24 0 45.83 60.929 1.00 0.00 4.929
245
241 CAGACACACACACACAACACA 182 21 0 47.62 57.434 1.00 0.00 3.566
246
242 ACAGACACACACACACAACACA 183 22 0 45.45 58.514 1.00 0.00 3.486
247
243 AACAGACACACACACACAACACA 184 23 0 43.48 60.064 1.00 0.00 3.064
248
244 CAACAGACACACACACACAACAC 185 23 0 47.83 60.064 1.00 0.00 3.064
249
245 ACAACAGACACACACACACAACAC 186 24 0 45.83 60.929 1.00 0.00 4.929
250
246 CAACAGACACACACACACAACA 185 22 0 45.45 59.121 1.00 0.00 2.879
251
247 ACAACAGACACACACACACAACA 186 23 0 43.48 60.064 1.00 0.00 3.064
252
248 CACAACAGACACACACACACAAC 187 23 0 47.83 60.064 1.00 0.00 3.064
253
249 ACACAACAGACACACACACACAAC 188 24 0 45.83 60.929 1.00 0.00 4.929
254
250 TACACAACAGACACACACACACAAC 189 25 0 44.00 60.904 2.00 0.00 5.904
255
251 ATACACAACAGACACACACACACAAC 190 26 0 42.31 61.121 2.00 0.00 7.121
256
252 TATACACAACAGACACACACACACAAC 191 27 0 40.74 61.092 4.00 0.00 8.092
257
253 CACAACAGACACACACACACAA 187 22 0 45.45 59.121 1.00 0.00 2.879
258
254 ACACAACAGACACACACACACAA 188 23 0 43.48 60.064 1.00 0.00 3.064
259
255 TACACAACAGACACACACACACAA 189 24 0 41.67 60.073 2.00 0.00 4.073
260
256 ATACACAACAGACACACACACACAA 190 25 0 40.00 60.344 2.00 0.00 5.344
261
257 TATACACAACAGACACACACACACAA 191 26 0 38.46 60.342 4.00 0.00 6.342
262
258 ATATACACAACAGACACACACACACAA 192 27 0 37.04 60.577 4.00 0.00 7.577
263
259 CACAACAGACACACACACACA 187 21 0 47.62 57.434 1.00 0.00 3.566
264
260 ACACAACAGACACACACACACA 188 22 0 45.45 58.514 1.00 0.00 3.486
265
261 TACACAACAGACACACACACACA 189 23 0 43.48 58.590 2.00 0.00 4.410
266
262 ATACACAACAGACACACACACACA 190 24 0 41.67 58.961 2.00 0.00 5.039
267
263 TATACACAACAGACACACACACACA 191 25 0 40.00 59.013 4.00 0.00 5.987
268
264 ATATACACAACAGACACACACACACA 192 26 0 38.46 59.329 4.00 0.00 6.671
269
265 TATATACACAACAGACACACACACACA 193 27 0 37.04 59.363 6.00 0.00 7.637
270
266 GTATATACACAACAGACACACACACAC 194 27 0 40.74 57.575 8.00 0.00 9.425
271
267 TGTATATACACAACAGACACACACACA 195 27 0 37.04 59.363 10.00 0.00 7.637
272
268 GTGTATATACACAACAGACACACACAC 196 27 0 40.74 57.575 12.00 0.00 9.425
273
269 CGTGTATATACACAACAGACACACACA 197 27 0 40.74 61.042 12.00 0.00 8.042
274
270 CGTGTATATACACAACAGACACACAC 197 26 0 42.31 58.508 12.00 0.00 7.492
275
271 ACGTGTATATACACAACAGACACACAC 198 27 0 40.74 59.293 12.00 0.00 7.707
276
272 CGTGTATATACACAACAGACACACA 197 25 0 40.00 57.664 12.00 0.00 7.336
277
273 ACGTGTATATACACAACAGACACACA 198 26 0 38.46 58.508 12.00 0.00 7.492
278
274 CACGTGTATATACACAACAGACACACA 199 27 0 40.74 61.042 12.00 0.00 8.042
279
275 CACGTGTATATACACAACAGACACAC 199 26 0 42.31 58.508 12.00 1.00 7.492
280
276 ACACGTGTATATACACAACAGACACAC 200 27 0 40.74 59.293 12.00 1.00 7.707
281
277 CACGTGTATATACACAACAGACACA 199 25 0 40.00 57.664 12.00 1.00 7.336
282
278 ACACGTGTATATACACAACAGACACA 200 26 0 38.46 58.508 12.00 1.00 7.492
283
279 CACACGTGTATATACACAACAGACACA 201 27 0 40.74 61.042 12.00 1.00 8.042
284
280 CACACGTGTATATACACAACAGACAC 201 26 0 42.31 58.508 12.00 3.00 7.492
285
281 ACACACGTGTATATACACAACAGACAC 202 27 0 40.74 59.293 12.00 3.00 7.707
286
282 CACACGTGTATATACACAACAGACA 201 25 0 40.00 57.664 12.00 1.00 7.336
287
283 ACACACGTGTATATACACAACAGACA 202 26 0 38.46 58.508 12.00 1.00 7.492
288
284 AACACACGTGTATATACACAACAGACA 203 27 0 37.04 59.748 12.00 1.00 7.252
289
285 AACACACGTGTATATACACAACAGAC 203 26 0 38.46 57.237 12.00 2.00 8.763
290
286 GAACACACGTGTATATACACAACAGAC 204 27 0 40.74 58.926 12.00 2.00 8.074
291
287 GAACACACGTGTATATACACAACAGA 204 26 0 38.46 58.139 12.00 4.00 7.861
292
288 AGAACACACGTGTATATACACAACAGA 205 27 0 37.04 59.049 12.00 4.00 7.951
293
289 AGAACACACGTGTATATACACAACAG 205 26 0 38.46 57.375 12.00 6.00 8.625
294
290 TAGAACACACGTGTATATACACAACAG 206 27 0 37.04 57.472 12.00 6.00 9.528
295
291 ACACACAACATACACTCTAGACACAG 237 26 0 42.31 57.508 6.00 1.00 8.492
296
292 CACACACAACATACACTCTAGACACAG 238 27 0 44.44 60.122 6.00 1.00 7.122
297
293 CACACACAACATACACTCTAGACACA 238 26 0 42.31 59.211 6.00 0.00 6.789
298
294 ACACACACAACATACACTCTAGACACA 239 27 0 40.74 60.001 6.00 0.00 7.001
299
295 ACACACACAACATACACTCTAGACAC 239 26 0 42.31 57.364 6.00 0.00 8.636
300
296 CACACACACAACATACACTCTAGACAC 240 27 0 44.44 60.001 6.00 0.00 7.001
301
297 CACACACACAACATACACTCTAGACA 240 26 0 42.31 59.211 6.00 2.00 6.789
302
298 ACACACACACAACATACACTCTAGACA 241 27 0 40.74 60.001 6.00 2.00 7.001
303
299 ACACACACACAACATACACTCTAGAC 241 26 0 42.31 57.364 6.00 4.00 8.636
304
300 CACACACACACAACATACACTCTAGAC 242 27 0 44.44 60.001 6.00 4.00 7.001
305
301 CACACACACACAACATACACTCTAGA 242 26 0 42.31 59.211 6.00 6.00 6.789
306
302 ACACACACACACAACATACACTCTAGA 243 27 0 40.74 60.001 6.00 6.00 7.001
307
303 CACACACACACAACATACACTCTAG 242 25 0 44.00 57.370 4.00 4.00 7.630
308
304 ACACACACACACAACATACACTCTAG 243 26 0 42.31 58.251 4.00 4.00 7.749
309
305 TACACACACACACAACATACACTCTAG 244 27 0 40.74 58.323 4.00 4.00 8.677
310
306 ACACACACACACAACATACACTCTA 243 25 0 40.00 57.217 3.00 3.00 7.783
311
307 TACACACACACACAACATACACTCTA 244 26 0 38.46 57.330 3.00 3.00 8.670
312
308 CTACACACACACACAACATACACTCTA 245 27 0 40.74 58.323 3.00 3.00 8.677
313
309 ACACACACACACAACATACACTCT 243 24 0 41.67 57.096 2.00 1.00 6.904
314
310 TACACACACACACAACATACACTCT 244 25 0 40.00 57.217 2.00 1.00 7.783
315
311 CTACACACACACACAACATACACTCT 245 26 0 42.31 58.251 2.00 1.00 7.749
316
312 TCTACACACACACACAACATACACTCT 246 27 0 40.74 60.001 2.00 1.00 7.001
317
313 CTACACACACACACAACATACACTC 245 25 0 44.00 57.217 2.00 0.00 7.783
318
314 TCTACACACACACACAACATACACTC 246 26 0 42.31 59.077 2.00 0.00 6.923
319
315 ATCTACACACACACACAACATACACTC 247 27 0 40.74 59.376 2.00 0.00 7.624
320
316 TCTACACACACACACAACATACACT 246 25 0 40.00 57.217 2.00 1.00 7.783
321
317 ATCTACACACACACACAACATACACT 247 26 0 38.46 57.620 2.00 1.00 8.380
322
318 GATCTACACACACACACAACATACACT 248 27 0 40.74 59.376 4.00 1.00 7.624
323
319 GATCTACACACACACACAACATACAC 248 26 0 42.31 58.431 4.00 0.00 7.569
324
320 GATCTACACACACACACAACATACA 248 25 0 40.00 57.548 4.00 0.00 7.452
added
removed
test/primer1_list_last/included_test_w_middle.rev
