1
ACCEPTABLE RIGHT PRIMERS
2
0-based # self self qual-
3
# sequence start ln N GC% Tm any end lity
4
0 GACCCCACGCACGCTAAG 158 18 0 66.67 62.798 3.00 3.00 4.798
5
1 GGAGAAAAGCCACTGGCA 240 18 0 55.56 59.918 7.00 5.00 2.082
6
2 TGGAGAAAAGCCACTGGCA 241 19 0 52.63 62.902 7.00 5.00 3.902
7
3 TGGAGAAAAGCCACTGGC 241 18 0 55.56 59.918 7.00 7.00 2.082
8
4 TTGGAGAAAAGCCACTGGC 242 19 0 52.63 61.313 7.00 7.00 2.313
9
5 GTTGGAGAAAAGCCACTGGC 243 20 0 55.00 62.088 7.00 7.00 2.088
10
6 TTGGAGAAAAGCCACTGG 242 18 0 50.00 57.278 5.00 5.00 4.722
11
7 GTTGGAGAAAAGCCACTGG 243 19 0 52.63 58.272 5.00 5.00 2.728
12
8 TGTTGGAGAAAAGCCACTGG 244 20 0 50.00 61.204 5.00 5.00 1.204
13
9 CTGTTGGAGAAAAGCCACTGG 245 21 0 52.38 62.093 5.00 5.00 3.093
14
10 TGTTGGAGAAAAGCCACTG 244 19 0 47.37 57.360 3.00 3.00 3.640
15
11 CTGTTGGAGAAAAGCCACTG 245 20 0 50.00 58.496 3.00 3.00 1.504
16
12 CCTGTTGGAGAAAAGCCACTG 246 21 0 52.38 62.093 3.00 3.00 3.093
17
13 CCTGTTGGAGAAAAGCCACT 246 20 0 50.00 59.328 3.00 1.00 0.672
18
14 TCCTGTTGGAGAAAAGCCACT 247 21 0 47.62 61.165 3.00 1.00 2.165
19
15 TTCCTGTTGGAGAAAAGCCACT 248 22 0 45.45 62.351 3.00 1.00 4.351
20
16 CCTGTTGGAGAAAAGCCAC 246 19 0 52.63 58.272 3.00 0.00 2.728
21
17 TCCTGTTGGAGAAAAGCCAC 247 20 0 50.00 60.232 3.00 0.00 0.232
22
18 TTCCTGTTGGAGAAAAGCCAC 248 21 0 47.62 61.523 3.00 0.00 2.523
23
19 CCTGTTGGAGAAAAGCCA 246 18 0 50.00 57.278 3.00 0.00 4.722
24
20 TCCTGTTGGAGAAAAGCCA 247 19 0 47.37 59.354 3.00 0.00 1.646
25
21 TTCCTGTTGGAGAAAAGCCA 248 20 0 45.00 60.745 3.00 0.00 0.745
26
22 TTCCTGTTGGAGAAAAGCC 248 19 0 47.37 57.850 3.00 1.00 3.150
added
removed
test/primer_list_last/SP01-02.txt.rev
