1
ACCEPTABLE INTERNAL OLIGOS
2
0-based # self self lib qual-
3
# sequence start ln N GC% Tm any end sim lity
4
0 AAACGCAAAGCACGCTCC 113 18 0 55.56 62.465 4.00 0.00 8.00 4.465
5
1 AAAACGCAAAGCACGCTC 112 18 0 50.00 60.142 4.00 2.00 9.00 2.142
6
2 CAAAACGCAAAGCACGCTC 111 19 0 52.63 62.977 4.00 2.00 9.00 3.977
7
3 TCCAAAACGCAAAGCACG 109 18 0 50.00 61.944 2.00 2.00 9.00 3.944
8
4 TTCCAAAACGCAAAGCAC 108 18 0 44.44 58.389 2.00 0.00 9.00 3.611
9
5 CTTCCAAAACGCAAAGCAC 107 19 0 47.37 59.454 3.00 0.00 9.00 1.546
10
6 ACTTCCAAAACGCAAAGCAC 106 20 0 45.00 60.292 3.00 0.00 9.00 0.292
11
7 AACTTCCAAAACGCAAAGCAC 105 21 0 42.86 61.528 3.00 0.00 10.00 2.528
12
8 TAACTTCCAAAACGCAAAGCAC 104 22 0 40.91 61.484 3.00 0.00 10.00 3.484
13
9 CTAACTTCCAAAACGCAAAGCAC 103 23 0 43.48 62.256 3.00 0.00 10.00 5.256
14
10 CTTCCAAAACGCAAAGCA 107 18 0 44.44 58.546 3.00 0.00 9.00 3.454
15
11 ACTTCCAAAACGCAAAGCA 106 19 0 42.11 59.454 3.00 0.00 9.00 1.546
16
12 AACTTCCAAAACGCAAAGCA 105 20 0 40.00 60.782 3.00 0.00 10.00 0.782
17
13 TAACTTCCAAAACGCAAAGCA 104 21 0 38.10 60.764 3.00 0.00 10.00 1.764
18
14 CTAACTTCCAAAACGCAAAGCA 103 22 0 40.91 61.593 3.00 0.00 10.00 3.593
19
15 AACTTCCAAAACGCAAAGC 105 19 0 42.11 58.013 3.00 2.00 10.00 2.987
20
16 TAACTTCCAAAACGCAAAGC 104 20 0 40.00 58.097 3.00 2.00 10.00 1.903
21
17 CTAACTTCCAAAACGCAAAGC 103 21 0 42.86 59.065 3.00 2.00 10.00 1.935
22
18 TCTAACTTCCAAAACGCAAAGC 102 22 0 40.91 60.748 3.00 2.00 10.00 2.748
23
19 GTCTAACTTCCAAAACGCAAAGC 101 23 0 43.48 61.438 3.00 2.00 10.00 4.438
24
20 AGTCTAACTTCCAAAACGCAAAGC 100 24 0 41.67 62.180 4.00 2.00 10.00 6.180
25
21 TCTAACTTCCAAAACGCAAAG 102 21 0 38.10 57.272 3.00 0.00 10.00 3.728
26
22 GTCTAACTTCCAAAACGCAAAG 101 22 0 40.91 58.127 3.00 0.00 10.00 3.873
27
23 AGTCTAACTTCCAAAACGCAAAG 100 23 0 39.13 59.045 4.00 0.00 10.00 3.955
28
24 AAGTCTAACTTCCAAAACGCAAAG 99 24 0 37.50 60.206 6.00 0.00 10.00 4.206
29
25 AAAGTCTAACTTCCAAAACGCAAAG 98 25 0 36.00 61.265 6.00 0.00 10.00 6.265
30
26 GTCTAACTTCCAAAACGCAAA 101 21 0 38.10 57.124 3.00 0.00 10.00 3.876
31
27 AGTCTAACTTCCAAAACGCAAA 100 22 0 36.36 58.127 4.00 0.00 10.00 3.873
32
28 AAGTCTAACTTCCAAAACGCAAA 99 23 0 34.78 59.378 6.00 0.00 10.00 3.622
33
29 AAAGTCTAACTTCCAAAACGCAAA 98 24 0 33.33 60.517 6.00 0.00 10.00 4.517
34
30 CAAAGTCTAACTTCCAAAACGCAAA 97 25 0 36.00 62.766 6.00 0.00 10.00 7.766
35
31 AAGTCTAACTTCCAAAACGCAA 99 22 0 36.36 58.127 6.00 0.00 10.00 3.873
36
32 AAAGTCTAACTTCCAAAACGCAA 98 23 0 34.78 59.378 6.00 0.00 10.00 3.622
37
33 CAAAGTCTAACTTCCAAAACGCAA 97 24 0 37.50 61.761 6.00 0.00 10.00 5.761
38
34 AAAGTCTAACTTCCAAAACGCA 98 22 0 36.36 58.127 6.00 0.00 10.00 3.873
39
35 CAAAGTCTAACTTCCAAAACGCA 97 23 0 39.13 60.658 6.00 0.00 10.00 3.658
40
36 TCAAAGTCTAACTTCCAAAACGCA 96 24 0 37.50 62.242 6.00 0.00 10.00 6.242
41
37 GTCAAAGTCTAACTTCCAAAACGCA 95 25 0 40.00 62.860 6.00 0.00 10.00 7.860
42
38 CAAAGTCTAACTTCCAAAACGC 97 22 0 40.91 58.127 6.00 2.00 10.00 3.873
43
39 TCAAAGTCTAACTTCCAAAACGC 96 23 0 39.13 59.821 6.00 2.00 10.00 3.179
44
40 GTCAAAGTCTAACTTCCAAAACGC 95 24 0 41.67 60.537 6.00 2.00 10.00 4.537
45
41 TGTCAAAGTCTAACTTCCAAAACGC 94 25 0 40.00 62.860 6.00 2.00 10.00 7.860
46
42 GTCAAAGTCTAACTTCCAAAACG 95 23 0 39.13 57.204 6.00 2.00 10.00 5.796
47
43 TGTCAAAGTCTAACTTCCAAAACG 94 24 0 37.50 59.738 6.00 2.00 10.00 4.262
48
44 TTGTCAAAGTCTAACTTCCAAAACG 93 25 0 36.00 60.875 6.00 2.00 10.00 5.875
49
45 CTGTTGTCAAAGTCTAACTTCCAA 90 24 0 37.50 57.684 6.00 2.00 9.00 6.316
50
46 TCTGTTGTCAAAGTCTAACTTCCAA 89 25 0 36.00 59.394 6.00 2.00 9.00 5.606
51
47 GTCTGTTGTCAAAGTCTAACTTCCAA 88 26 0 38.46 60.127 6.00 2.00 9.00 6.127
52
48 AGTCTGTTGTCAAAGTCTAACTTCCAA 87 27 0 37.04 60.916 7.00 2.00 10.00 7.916
53
49 AGTCTGTTGTCAAAGTCTAACTTCC 87 25 0 40.00 57.318 7.00 2.00 10.00 7.682
54
50 GAGTCTGTTGTCAAAGTCTAACTTCC 86 26 0 42.31 58.988 7.00 2.00 10.00 7.012
55
51 TGAGTCTGTTGTCAAAGTCTAACTTCC 85 27 0 40.74 61.367 7.00 2.00 10.00 8.367
56
52 CAACTCACTGTATGAGTCTGTTGTC 73 25 0 44.00 57.993 6.00 1.00 11.00 7.007
57
53 CCAACTCACTGTATGAGTCTGTTGTC 72 26 0 46.15 61.357 6.00 2.00 11.00 7.357
58
54 AAACGCCAACTCACTGTATGA 67 21 0 42.86 58.296 3.00 2.00 10.00 2.704
59
55 GAAACGCCAACTCACTGTATGA 66 22 0 45.45 60.176 3.00 2.00 10.00 2.176
60
56 AGAAACGCCAACTCACTGTATGA 65 23 0 43.48 61.075 3.00 2.00 10.00 4.075
61
57 TAGAAACGCCAACTCACTGTATGA 64 24 0 41.67 61.047 3.00 2.00 10.00 5.047
62
58 ATAGAAACGCCAACTCACTGTATGA 63 25 0 40.00 61.238 3.00 2.00 10.00 6.238
63
59 GATAGAAACGCCAACTCACTGTATGA 62 26 0 42.31 62.768 3.00 2.00 10.00 8.768
64
60 GAAACGCCAACTCACTGTATG 66 21 0 47.62 58.296 3.00 1.00 10.00 2.704
65
61 AGAAACGCCAACTCACTGTATG 65 22 0 45.45 59.309 3.00 1.00 10.00 2.691
66
62 TAGAAACGCCAACTCACTGTATG 64 23 0 43.48 59.344 3.00 1.00 10.00 3.656
67
63 ATAGAAACGCCAACTCACTGTATG 63 24 0 41.67 59.623 3.00 1.00 10.00 4.377
68
64 GATAGAAACGCCAACTCACTGTATG 62 25 0 44.00 61.238 3.00 1.00 10.00 6.238
69
65 ATAGAAACGCCAACTCACTGTAT 63 23 0 39.13 57.058 3.00 2.00 10.00 5.942
70
66 GATAGAAACGCCAACTCACTGTAT 62 24 0 41.67 58.785 3.00 2.00 10.00 5.215
71
67 TGATAGAAACGCCAACTCACTGTAT 61 25 0 40.00 61.238 3.00 2.00 10.00 6.238
72
68 CTGATAGAAACGCCAACTCACTGTAT 60 26 0 42.31 61.990 3.00 2.00 10.00 7.990
73
69 GATAGAAACGCCAACTCACTGTA 62 23 0 43.48 58.464 3.00 2.00 10.00 4.536
74
70 TGATAGAAACGCCAACTCACTGTA 61 24 0 41.67 61.047 3.00 2.00 10.00 5.047
75
71 CTGATAGAAACGCCAACTCACTGTA 60 25 0 44.00 61.842 3.00 2.00 10.00 6.842
76
72 TGATAGAAACGCCAACTCACT 61 21 0 42.86 57.496 3.00 1.00 10.00 3.504
77
73 CTGATAGAAACGCCAACTCACT 60 22 0 45.45 58.536 3.00 1.00 10.00 3.464
78
74 CCTGATAGAAACGCCAACTCACT 59 23 0 47.83 61.863 3.00 1.00 10.00 4.863
79
75 ACCTGATAGAAACGCCAACTCACT 58 24 0 45.83 62.526 3.00 1.00 11.00 6.526
80
76 CTGATAGAAACGCCAACTCAC 60 21 0 47.62 57.496 3.00 0.00 10.00 3.504
81
77 CCTGATAGAAACGCCAACTCAC 59 22 0 50.00 61.033 3.00 0.00 10.00 3.033
82
78 ACCTGATAGAAACGCCAACTCAC 58 23 0 47.83 61.755 3.00 0.00 11.00 4.755
83
79 CCTGATAGAAACGCCAACTCA 59 21 0 47.62 60.256 3.00 1.00 10.00 1.256
84
80 ACCTGATAGAAACGCCAACTCA 58 22 0 45.45 61.033 3.00 1.00 11.00 3.033
85
81 GACCTGATAGAAACGCCAACTCA 57 23 0 47.83 62.713 3.00 1.00 11.00 5.713
86
82 CCTGATAGAAACGCCAACTC 59 20 0 50.00 57.422 2.00 0.00 10.00 2.578
87
83 ACCTGATAGAAACGCCAACTC 58 21 0 47.62 58.332 2.00 0.00 11.00 2.668
88
84 GACCTGATAGAAACGCCAACTC 57 22 0 50.00 60.137 2.00 0.00 11.00 2.137
89
85 TGACCTGATAGAAACGCCAACTC 56 23 0 47.83 62.713 2.00 0.00 11.00 5.713
90
86 ATGACCTGATAGAAACGCCAACTC 55 24 0 45.83 62.827 2.00 0.00 11.00 6.827
91
87 TATGACCTGATAGAAACGCCAACTC 54 25 0 44.00 62.743 4.00 0.00 11.00 7.743
92
88 GACCTGATAGAAACGCCAACT 57 21 0 47.62 58.332 2.00 1.00 11.00 2.668
93
89 TGACCTGATAGAAACGCCAACT 56 22 0 45.45 61.033 2.00 1.00 11.00 3.033
94
90 ATGACCTGATAGAAACGCCAACT 55 23 0 43.48 61.233 2.00 1.00 11.00 4.233
95
91 TATGACCTGATAGAAACGCCAACT 54 24 0 41.67 61.201 4.00 1.00 11.00 5.201
96
92 CTATGACCTGATAGAAACGCCAACT 53 25 0 44.00 61.959 6.00 1.00 11.00 6.959
97
93 GACCTGATAGAAACGCCAAC 57 20 0 50.00 57.265 2.00 0.00 11.00 2.735
98
94 TGACCTGATAGAAACGCCAAC 56 21 0 47.62 60.125 2.00 0.00 11.00 1.125
99
95 ATGACCTGATAGAAACGCCAAC 55 22 0 45.45 60.381 2.00 0.00 11.00 2.381
100
96 TATGACCTGATAGAAACGCCAAC 54 23 0 43.48 60.377 4.00 0.00 11.00 3.377
101
97 CTATGACCTGATAGAAACGCCAAC 53 24 0 45.83 61.201 6.00 0.00 11.00 5.201
102
98 TCTATGACCTGATAGAAACGCCAAC 52 25 0 44.00 62.743 8.00 0.00 11.00 7.743
103
99 TGACCTGATAGAAACGCCAA 56 20 0 45.00 59.272 2.00 0.00 11.00 0.728
104
100 ATGACCTGATAGAAACGCCAA 55 21 0 42.86 59.583 2.00 0.00 11.00 1.417
105
101 TATGACCTGATAGAAACGCCAA 54 22 0 40.91 59.609 4.00 0.00 11.00 2.391
106
102 CTATGACCTGATAGAAACGCCAA 53 23 0 43.48 60.495 6.00 0.00 11.00 3.495
107
103 TCTATGACCTGATAGAAACGCCAA 52 24 0 41.67 62.110 8.00 0.00 11.00 6.110
108
104 TGACCTGATAGAAACGCCA 56 19 0 47.37 57.759 2.00 0.00 11.00 3.241
109
105 ATGACCTGATAGAAACGCCA 55 20 0 45.00 58.173 2.00 0.00 11.00 1.827
110
106 TATGACCTGATAGAAACGCCA 54 21 0 42.86 58.254 4.00 0.00 11.00 2.746
111
107 CTATGACCTGATAGAAACGCCA 53 22 0 45.45 59.248 6.00 0.00 11.00 2.752
112
108 TCTATGACCTGATAGAAACGCCA 52 23 0 43.48 60.980 8.00 0.00 11.00 3.980
113
109 TTCTATGACCTGATAGAAACGCCA 51 24 0 41.67 62.110 10.00 0.00 11.00 6.110
114
110 TCTATGACCTGATAGAAACGCC 52 22 0 45.45 58.353 8.00 1.00 11.00 3.647
115
111 TTCTATGACCTGATAGAAACGCC 51 23 0 43.48 59.643 10.00 1.00 11.00 3.357
116
112 TTTCTATGACCTGATAGAAACGCC 50 24 0 41.67 60.811 12.00 1.00 11.00 4.811
117
113 TTTTCTATGACCTGATAGAAACGCC 49 25 0 40.00 61.874 12.00 2.00 11.00 6.874
118
114 TTTCTATGACCTGATAGAAACGC 50 23 0 39.13 57.615 12.00 2.00 11.00 5.385
119
115 CAATCAAATTTTTTCTATGACCTGAT 39 26 0 26.92 58.947 6.00 2.00 11.00 7.053
120
116 GCAATCAAATTTTTTCTATGACCTGAT 38 27 0 29.63 61.857 6.00 2.00 11.00 8.857
121
117 CAATCAAATTTTTTCTATGACCTGA 39 25 0 28.00 58.665 6.00 1.00 11.00 6.335
122
118 GCAATCAAATTTTTTCTATGACCTGA 38 26 0 30.77 61.716 6.00 1.00 11.00 7.716
123
119 CAATCAAATTTTTTCTATGACCTG 39 24 0 29.17 57.024 6.00 1.00 11.00 6.976
124
120 GCAATCAAATTTTTTCTATGACCTG 38 25 0 32.00 60.235 6.00 1.00 11.00 5.235
125
121 GCAATCAAATTTTTTCTATGACCT 38 24 0 29.17 57.882 6.00 1.00 11.00 6.118
126
122 CGCAATCAAATTTTTTCTATGACCT 37 25 0 32.00 61.717 6.00 1.00 11.00 6.717
127
123 CGCAATCAAATTTTTTCTATGACC 37 24 0 33.33 60.984 6.00 0.00 11.00 4.984
128
124 TCGCAATCAAATTTTTTCTATGACC 36 25 0 32.00 62.463 6.00 0.00 11.00 7.463
129
125 ATCGCAATCAAATTTTTTCTATGACC 35 26 0 30.77 62.574 6.00 0.00 11.00 8.574
130
126 CGCAATCAAATTTTTTCTATGAC 37 23 0 30.43 57.905 6.00 1.00 10.00 5.095
131
127 TCGCAATCAAATTTTTTCTATGAC 36 24 0 29.17 59.542 6.00 1.00 10.00 4.458
132
128 ATCGCAATCAAATTTTTTCTATGAC 35 25 0 28.00 59.789 6.00 2.00 10.00 5.211
133
129 AATCGCAATCAAATTTTTTCTATGAC 34 26 0 26.92 60.843 6.00 1.00 10.00 6.843
134
130 GAATCGCAATCAAATTTTTTCTATGAC 33 27 0 29.63 62.235 6.00 1.00 10.00 9.235
135
131 CGCAATCAAATTTTTTCTATGA 37 22 0 27.27 57.077 6.00 2.00 10.00 4.923
136
132 TCGCAATCAAATTTTTTCTATGA 36 23 0 26.09 58.793 6.00 2.00 10.00 4.207
137
133 AATCGCAATCAAATTTTTTCTATGA 34 25 0 24.00 60.202 6.00 2.00 10.00 5.202
138
134 GAATCGCAATCAAATTTTTTCTATGA 33 26 0 26.92 61.653 6.00 2.00 10.00 7.653
139
135 TCGCAATCAAATTTTTTCTATG 36 22 0 27.27 57.077 6.00 2.00 10.00 4.923
140
136 ATCGCAATCAAATTTTTTCTATG 35 23 0 26.09 57.456 6.00 2.00 10.00 5.544
141
137 AATCGCAATCAAATTTTTTCTATG 34 24 0 25.00 58.677 6.00 2.00 10.00 5.323
142
138 GAATCGCAATCAAATTTTTTCTATG 33 25 0 28.00 60.202 6.00 2.00 10.00 5.202
143
139 TGAATCGCAATCAAATTTTTTCTATG 32 26 0 26.92 62.397 6.00 2.00 11.00 8.397
144
140 ATGAATCGCAATCAAATTTTTTCTATG 31 27 0 25.93 62.507 6.00 2.00 12.00 9.507
145
141 GAATCGCAATCAAATTTTTTCTA 33 23 0 26.09 57.561 6.00 2.00 10.00 5.439
146
142 TGAATCGCAATCAAATTTTTTCTA 32 24 0 25.00 59.977 6.00 2.00 11.00 4.023
147
143 ATGAATCGCAATCAAATTTTTTCTA 31 25 0 24.00 60.202 6.00 2.00 12.00 5.202
148
144 TATGAATCGCAATCAAATTTTTTCTA 30 26 0 23.08 60.204 6.00 2.00 12.00 6.204
149
145 TTATGAATCGCAATCAAATTTTTTCTA 29 27 0 22.22 61.199 6.00 2.00 12.00 8.199
150
146 GAATCGCAATCAAATTTTTTCT 33 22 0 27.27 57.467 6.00 2.00 10.00 4.533
151
147 TGAATCGCAATCAAATTTTTTCT 32 23 0 26.09 59.967 6.00 2.00 11.00 3.033
152
148 ATGAATCGCAATCAAATTTTTTCT 31 24 0 25.00 60.199 6.00 2.00 12.00 4.199
153
149 TATGAATCGCAATCAAATTTTTTCT 30 25 0 24.00 60.202 6.00 2.00 12.00 5.202
154
150 TTATGAATCGCAATCAAATTTTTTCT 29 26 0 23.08 61.226 6.00 2.00 12.00 7.226
155
151 ATTATGAATCGCAATCAAATTTTTTCT 28 27 0 22.22 61.382 6.00 2.00 12.00 8.382
156
152 TGAATCGCAATCAAATTTTTTC 32 22 0 27.27 59.091 6.00 2.00 11.00 2.909
157
153 ATGAATCGCAATCAAATTTTTTC 31 23 0 26.09 59.374 6.00 2.00 12.00 3.626
158
154 TATGAATCGCAATCAAATTTTTTC 30 24 0 25.00 59.404 6.00 2.00 12.00 4.596
159
155 TTATGAATCGCAATCAAATTTTTTC 29 25 0 24.00 60.502 6.00 2.00 12.00 5.502
160
156 ATTATGAATCGCAATCAAATTTTTTC 28 26 0 23.08 60.694 6.00 2.00 12.00 6.694
161
157 AATTATGAATCGCAATCAAATTTTTTC 27 27 0 22.22 61.652 8.00 2.00 12.00 8.652
added
removed
test/primer_list_last/SP01-06-with-repeat3.int
