6
PRIMER_TASK=pick_pcr_primers
7
PRIMER_SEQUENCE_ID=pcr_primers
8
SEQUENCE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
10
PRIMER_LEFT_EXPLAIN=considered 10, low tm 7, ok 3
11
PRIMER_RIGHT_EXPLAIN=considered 1, ok 1
12
PRIMER_PAIR_EXPLAIN=considered 3, ok 3
13
PRIMER_PAIR_PENALTY=3.4323
14
PRIMER_LEFT_PENALTY=1.117633
15
PRIMER_RIGHT_PENALTY=2.314641
16
PRIMER_LEFT_SEQUENCE=TCCTACAGCTGTGGGAAAATG
17
PRIMER_RIGHT_SEQUENCE=CAGTGTGTACATGGCGGC
21
PRIMER_RIGHT_TM=59.685
22
PRIMER_LEFT_GC_PERCENT=47.619
23
PRIMER_RIGHT_GC_PERCENT=61.111
24
PRIMER_LEFT_SELF_ANY=12.00
25
PRIMER_RIGHT_SELF_ANY=6.00
26
PRIMER_LEFT_SELF_END=0.00
27
PRIMER_RIGHT_SELF_END=3.00
28
PRIMER_LEFT_END_STABILITY=7.2000
29
PRIMER_RIGHT_END_STABILITY=12.9000
30
PRIMER_PAIR_COMPL_ANY=4.00
31
PRIMER_PAIR_COMPL_END=1.00
32
PRIMER_PRODUCT_SIZE=161
33
PRIMER_PAIR_PENALTY_1=4.0864
34
PRIMER_LEFT_1_PENALTY=1.771733
35
PRIMER_RIGHT_1_PENALTY=2.314641
36
PRIMER_LEFT_1_SEQUENCE=CCTACAGCTGTGGGAAAATG
37
PRIMER_RIGHT_1_SEQUENCE=CAGTGTGTACATGGCGGC
40
PRIMER_LEFT_1_TM=58.228
41
PRIMER_RIGHT_1_TM=59.685
42
PRIMER_LEFT_1_GC_PERCENT=50.000
43
PRIMER_RIGHT_1_GC_PERCENT=61.111
44
PRIMER_LEFT_1_SELF_ANY=10.00
45
PRIMER_RIGHT_1_SELF_ANY=6.00
46
PRIMER_LEFT_1_SELF_END=0.00
47
PRIMER_RIGHT_1_SELF_END=3.00
48
PRIMER_LEFT_1_END_STABILITY=7.2000
49
PRIMER_RIGHT_1_END_STABILITY=12.9000
50
PRIMER_PAIR_1_COMPL_ANY=4.00
51
PRIMER_PAIR_1_COMPL_END=1.00
52
PRIMER_PRODUCT_SIZE_1=160
53
PRIMER_PAIR_PENALTY_2=5.0463
54
PRIMER_LEFT_2_PENALTY=2.731698
55
PRIMER_RIGHT_2_PENALTY=2.314641
56
PRIMER_LEFT_2_SEQUENCE=TCCTACAGCTGTGGGAAAAT
57
PRIMER_RIGHT_2_SEQUENCE=CAGTGTGTACATGGCGGC
60
PRIMER_LEFT_2_TM=57.268
61
PRIMER_RIGHT_2_TM=59.685
62
PRIMER_LEFT_2_GC_PERCENT=45.000
63
PRIMER_RIGHT_2_GC_PERCENT=61.111
64
PRIMER_LEFT_2_SELF_ANY=12.00
65
PRIMER_RIGHT_2_SELF_ANY=6.00
66
PRIMER_LEFT_2_SELF_END=2.00
67
PRIMER_RIGHT_2_SELF_END=3.00
68
PRIMER_LEFT_2_END_STABILITY=7.2000
69
PRIMER_RIGHT_2_END_STABILITY=12.9000
70
PRIMER_PAIR_2_COMPL_ANY=4.00
71
PRIMER_PAIR_2_COMPL_END=0.00
72
PRIMER_PRODUCT_SIZE_2=161
74
PRIMER_SEQUENCE_ID=pcr_primers_and_io
75
PRIMER_TASK=pick_pcr_primers_and_hyb_PROBE
76
SEQUENCE=NATCCANGCTGTTTCACACTTACGGAAATGGANGACTNCCCATAGCTNCTGGGCTNTTATCCAAAAAACATTCTCTNNNTGNTGCTTATTGGCNTCTCNAAGTGTGCNNACTTCATGGGCCTACACANAATTGNNCANGGGCTTAGCGTGCGTGGGGTCCCTNGGTAACATNGGCATCTCTGCACTTCNNGGTNNCCTTCCTTCTNACCAAGGGGTATCTGGNTGCCAGTGGCTTTTCTCCAACAGGAANGGGGGTATGTGCATTNGCAGACATCAATGANCATGAGAGATATNAATAGG
77
PRIMER_LEFT_EXPLAIN=considered 281, too many Ns 210, low tm 30, high tm 29, ok 12
78
PRIMER_RIGHT_EXPLAIN=considered 284, too many Ns 210, low tm 7, high tm 44, ok 23
79
PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 393, too many Ns 280, low tm 30, high tm 46, long poly-x seq 3, ok 34
80
PRIMER_PAIR_EXPLAIN=considered 7, ok 7
81
PRIMER_PAIR_PENALTY=2.3342
82
PRIMER_LEFT_PENALTY=2.101896
83
PRIMER_RIGHT_PENALTY=0.232299
84
PRIMER_INTERNAL_OLIGO_PENALTY=4.798289
85
PRIMER_LEFT_SEQUENCE=TGTTTCACACTTACGGAAATGG
86
PRIMER_RIGHT_SEQUENCE=TCCTGTTGGAGAAAAGCCAC
87
PRIMER_INTERNAL_OLIGO_SEQUENCE=CTTAGCGTGCGTGGGGTC
90
PRIMER_INTERNAL_OLIGO=141,18
92
PRIMER_RIGHT_TM=60.232
93
PRIMER_INTERNAL_OLIGO_TM=62.798
94
PRIMER_LEFT_GC_PERCENT=40.909
95
PRIMER_RIGHT_GC_PERCENT=50.000
96
PRIMER_INTERNAL_OLIGO_GC_PERCENT=66.667
97
PRIMER_LEFT_SELF_ANY=6.00
98
PRIMER_RIGHT_SELF_ANY=3.00
99
PRIMER_INTERNAL_OLIGO_SELF_ANY=3.00
100
PRIMER_LEFT_SELF_END=4.00
101
PRIMER_RIGHT_SELF_END=0.00
102
PRIMER_INTERNAL_OLIGO_SELF_END=1.00
103
PRIMER_LEFT_END_STABILITY=8.4000
104
PRIMER_RIGHT_END_STABILITY=9.4000
105
PRIMER_PAIR_COMPL_ANY=4.00
106
PRIMER_PAIR_COMPL_END=2.00
107
PRIMER_PRODUCT_SIZE=239
108
PRIMER_PAIR_PENALTY_1=2.7735
109
PRIMER_LEFT_1_PENALTY=2.101896
110
PRIMER_RIGHT_1_PENALTY=0.671597
111
PRIMER_INTERNAL_OLIGO_1_PENALTY=4.798289
112
PRIMER_LEFT_1_SEQUENCE=TGTTTCACACTTACGGAAATGG
113
PRIMER_RIGHT_1_SEQUENCE=CCTGTTGGAGAAAAGCCACT
114
PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CTTAGCGTGCGTGGGGTC
116
PRIMER_RIGHT_1=246,20
117
PRIMER_INTERNAL_OLIGO_1=141,18
118
PRIMER_LEFT_1_TM=59.898
119
PRIMER_RIGHT_1_TM=59.328
120
PRIMER_INTERNAL_OLIGO_1_TM=62.798
121
PRIMER_LEFT_1_GC_PERCENT=40.909
122
PRIMER_RIGHT_1_GC_PERCENT=50.000
123
PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=66.667
124
PRIMER_LEFT_1_SELF_ANY=6.00
125
PRIMER_RIGHT_1_SELF_ANY=3.00
126
PRIMER_INTERNAL_OLIGO_1_SELF_ANY=3.00
127
PRIMER_LEFT_1_SELF_END=4.00
128
PRIMER_RIGHT_1_SELF_END=1.00
129
PRIMER_INTERNAL_OLIGO_1_SELF_END=1.00
130
PRIMER_LEFT_1_END_STABILITY=8.4000
131
PRIMER_RIGHT_1_END_STABILITY=7.9000
132
PRIMER_PAIR_1_COMPL_ANY=4.00
133
PRIMER_PAIR_1_COMPL_END=3.00
134
PRIMER_PRODUCT_SIZE_1=238
135
PRIMER_PAIR_PENALTY_2=2.8468
136
PRIMER_LEFT_2_PENALTY=2.101896
137
PRIMER_RIGHT_2_PENALTY=0.744925
138
PRIMER_INTERNAL_OLIGO_2_PENALTY=4.798289
139
PRIMER_LEFT_2_SEQUENCE=TGTTTCACACTTACGGAAATGG
140
PRIMER_RIGHT_2_SEQUENCE=TTCCTGTTGGAGAAAAGCCA
141
PRIMER_INTERNAL_OLIGO_2_SEQUENCE=CTTAGCGTGCGTGGGGTC
143
PRIMER_RIGHT_2=248,20
144
PRIMER_INTERNAL_OLIGO_2=141,18
145
PRIMER_LEFT_2_TM=59.898
146
PRIMER_RIGHT_2_TM=60.745
147
PRIMER_INTERNAL_OLIGO_2_TM=62.798
148
PRIMER_LEFT_2_GC_PERCENT=40.909
149
PRIMER_RIGHT_2_GC_PERCENT=45.000
150
PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=66.667
151
PRIMER_LEFT_2_SELF_ANY=6.00
152
PRIMER_RIGHT_2_SELF_ANY=3.00
153
PRIMER_INTERNAL_OLIGO_2_SELF_ANY=3.00
154
PRIMER_LEFT_2_SELF_END=4.00
155
PRIMER_RIGHT_2_SELF_END=0.00
156
PRIMER_INTERNAL_OLIGO_2_SELF_END=1.00
157
PRIMER_LEFT_2_END_STABILITY=8.4000
158
PRIMER_RIGHT_2_END_STABILITY=9.7000
159
PRIMER_PAIR_2_COMPL_ANY=4.00
160
PRIMER_PAIR_2_COMPL_END=3.00
161
PRIMER_PRODUCT_SIZE_2=240
162
PRIMER_PAIR_PENALTY_3=3.0984
163
PRIMER_LEFT_3_PENALTY=2.866131
164
PRIMER_RIGHT_3_PENALTY=0.232299
165
PRIMER_INTERNAL_OLIGO_3_PENALTY=4.798289
166
PRIMER_LEFT_3_SEQUENCE=TTTCACACTTACGGAAATGGA
167
PRIMER_RIGHT_3_SEQUENCE=TCCTGTTGGAGAAAAGCCAC
168
PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CTTAGCGTGCGTGGGGTC
170
PRIMER_RIGHT_3=247,20
171
PRIMER_INTERNAL_OLIGO_3=141,18
172
PRIMER_LEFT_3_TM=58.134
173
PRIMER_RIGHT_3_TM=60.232
174
PRIMER_INTERNAL_OLIGO_3_TM=62.798
175
PRIMER_LEFT_3_GC_PERCENT=38.095
176
PRIMER_RIGHT_3_GC_PERCENT=50.000
177
PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=66.667
178
PRIMER_LEFT_3_SELF_ANY=6.00
179
PRIMER_RIGHT_3_SELF_ANY=3.00
180
PRIMER_INTERNAL_OLIGO_3_SELF_ANY=3.00
181
PRIMER_LEFT_3_SELF_END=6.00
182
PRIMER_RIGHT_3_SELF_END=0.00
183
PRIMER_INTERNAL_OLIGO_3_SELF_END=1.00
184
PRIMER_LEFT_3_END_STABILITY=8.1000
185
PRIMER_RIGHT_3_END_STABILITY=9.4000
186
PRIMER_PAIR_3_COMPL_ANY=4.00
187
PRIMER_PAIR_3_COMPL_END=1.00
188
PRIMER_PRODUCT_SIZE_3=237
189
PRIMER_PAIR_PENALTY_4=3.2356
190
PRIMER_LEFT_4_PENALTY=3.003259
191
PRIMER_RIGHT_4_PENALTY=0.232299
192
PRIMER_INTERNAL_OLIGO_4_PENALTY=4.798289
193
PRIMER_LEFT_4_SEQUENCE=GTTTCACACTTACGGAAATGGA
194
PRIMER_RIGHT_4_SEQUENCE=TCCTGTTGGAGAAAAGCCAC
195
PRIMER_INTERNAL_OLIGO_4_SEQUENCE=CTTAGCGTGCGTGGGGTC
197
PRIMER_RIGHT_4=247,20
198
PRIMER_INTERNAL_OLIGO_4=141,18
199
PRIMER_LEFT_4_TM=58.997
200
PRIMER_RIGHT_4_TM=60.232
201
PRIMER_INTERNAL_OLIGO_4_TM=62.798
202
PRIMER_LEFT_4_GC_PERCENT=40.909
203
PRIMER_RIGHT_4_GC_PERCENT=50.000
204
PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=66.667
205
PRIMER_LEFT_4_SELF_ANY=6.00
206
PRIMER_RIGHT_4_SELF_ANY=3.00
207
PRIMER_INTERNAL_OLIGO_4_SELF_ANY=3.00
208
PRIMER_LEFT_4_SELF_END=6.00
209
PRIMER_RIGHT_4_SELF_END=0.00
210
PRIMER_INTERNAL_OLIGO_4_SELF_END=1.00
211
PRIMER_LEFT_4_END_STABILITY=8.1000
212
PRIMER_RIGHT_4_END_STABILITY=9.4000
213
PRIMER_PAIR_4_COMPL_ANY=4.00
214
PRIMER_PAIR_4_COMPL_END=1.00
215
PRIMER_PRODUCT_SIZE_4=238
217
PRIMER_SEQUENCE_ID=left_only
218
PRIMER_TASK=pick_left_only
220
SEQUENCE=GNATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC
221
PRIMER_LEFT_EXPLAIN=considered 1121, too many Ns 38, overlap target 23, low tm 42, high tm 866, ok 152
222
PRIMER_LEFT_PENALTY=0.0408
223
PRIMER_LEFT_SEQUENCE=TTAGAAGGGGTGTCTGGGTG
225
PRIMER_LEFT_TM=59.959
226
PRIMER_LEFT_GC_PERCENT=55.000
227
PRIMER_LEFT_SELF_ANY=3.00
228
PRIMER_LEFT_SELF_END=0.00
229
PRIMER_LEFT_END_STABILITY=9.4000
230
PRIMER_LEFT_1_PENALTY=0.2717
231
PRIMER_LEFT_1_SEQUENCE=CTGCAGGTTTAGAAGGGGTG
233
PRIMER_LEFT_1_TM=59.728
234
PRIMER_LEFT_1_GC_PERCENT=55.000
235
PRIMER_LEFT_1_SELF_ANY=6.00
236
PRIMER_LEFT_1_SELF_END=0.00
237
PRIMER_LEFT_1_END_STABILITY=9.4000
238
PRIMER_LEFT_2_PENALTY=0.2717
239
PRIMER_LEFT_2_SEQUENCE=CACCCTGCAGGTTTAGAAGG
241
PRIMER_LEFT_2_TM=59.728
242
PRIMER_LEFT_2_GC_PERCENT=55.000
243
PRIMER_LEFT_2_SELF_ANY=8.00
244
PRIMER_LEFT_2_SELF_END=0.00
245
PRIMER_LEFT_2_END_STABILITY=8.2000
246
PRIMER_LEFT_3_PENALTY=0.3125
247
PRIMER_LEFT_3_SEQUENCE=AGCACACCCTGCAGGTTTAG
249
PRIMER_LEFT_3_TM=60.312
250
PRIMER_LEFT_3_GC_PERCENT=55.000
251
PRIMER_LEFT_3_SELF_ANY=8.00
252
PRIMER_LEFT_3_SELF_END=6.00
253
PRIMER_LEFT_3_END_STABILITY=6.3000
254
PRIMER_LEFT_4_PENALTY=0.4079
255
PRIMER_LEFT_4_SEQUENCE=TGCAGGTTTAGAAGGGGTGT
257
PRIMER_LEFT_4_TM=59.592
258
PRIMER_LEFT_4_GC_PERCENT=50.000
259
PRIMER_LEFT_4_SELF_ANY=4.00
260
PRIMER_LEFT_4_SELF_END=0.00
261
PRIMER_LEFT_4_END_STABILITY=7.6000
263
PRIMER_SEQUENCE_ID=right_only
264
PRIMER_TASK=PICK_right_only
266
SEQUENCE=GANCAATGCANGTAATGANGTTGGCANAATTATGAATCGCAATCAAATTTTTTCTATGACCTGATAGAAACGCCAACTCACTGTATGAGTCTGTTGTCAAAGTCTAACTTCCAAAACGCAAAGCACGCTCCCGATC
267
PRIMER_RIGHT_EXPLAIN=considered 623, too many Ns 27, overlap target 36, GC content failed 5, low tm 230, high tm 145, high end compl 6, ok 174
268
PRIMER_RIGHT_PENALTY=0.2917
269
PRIMER_RIGHT_SEQUENCE=GTGCTTTGCGTTTTGGAAGT
271
PRIMER_RIGHT_TM=60.292
272
PRIMER_RIGHT_GC_PERCENT=45.000
273
PRIMER_RIGHT_SELF_ANY=3.00
274
PRIMER_RIGHT_SELF_END=1.00
275
PRIMER_RIGHT_END_STABILITY=6.4000
276
PRIMER_RIGHT_1_PENALTY=0.7280
277
PRIMER_RIGHT_1_SEQUENCE=TTGGCGTTTCTATCAGGTCA
279
PRIMER_RIGHT_1_TM=59.272
280
PRIMER_RIGHT_1_GC_PERCENT=45.000
281
PRIMER_RIGHT_1_SELF_ANY=2.00
282
PRIMER_RIGHT_1_SELF_END=1.00
283
PRIMER_RIGHT_1_END_STABILITY=7.9000
284
PRIMER_RIGHT_2_PENALTY=0.7821
285
PRIMER_RIGHT_2_SEQUENCE=TGCTTTGCGTTTTGGAAGTT
286
PRIMER_RIGHT_2=124,20
287
PRIMER_RIGHT_2_TM=60.782
288
PRIMER_RIGHT_2_GC_PERCENT=40.000
289
PRIMER_RIGHT_2_SELF_ANY=3.00
290
PRIMER_RIGHT_2_SELF_END=3.00
291
PRIMER_RIGHT_2_END_STABILITY=6.7000
292
PRIMER_RIGHT_3_PENALTY=1.1251
293
PRIMER_RIGHT_3_SEQUENCE=GTTGGCGTTTCTATCAGGTCA
295
PRIMER_RIGHT_3_TM=60.125
296
PRIMER_RIGHT_3_GC_PERCENT=47.619
297
PRIMER_RIGHT_3_SELF_ANY=2.00
298
PRIMER_RIGHT_3_SELF_END=1.00
299
PRIMER_RIGHT_3_END_STABILITY=7.9000
300
PRIMER_RIGHT_4_PENALTY=1.2557
301
PRIMER_RIGHT_4_SEQUENCE=TGAGTTGGCGTTTCTATCAGG
303
PRIMER_RIGHT_4_TM=60.256
304
PRIMER_RIGHT_4_GC_PERCENT=47.619
305
PRIMER_RIGHT_4_SELF_ANY=3.00
306
PRIMER_RIGHT_4_SELF_END=0.00
307
PRIMER_RIGHT_4_END_STABILITY=8.2000
309
PRIMER_SEQUENCE_ID=hyb_probe_only
310
PRIMER_TASK=pick_HYB_probe_ONly
311
PRIMER_INTERNAL_OLIGO_EXCLUDED_REGION=90,20
312
SEQUENCE=GCATCTGCACCCTGTAAACCCTGGCTCCTGCCTCTCCGGGACACCCCACTGAGGTCAGCACACCCTGCAGGTTTAGAAGGGGTGTCTGGGTGCATTTGGTGACACCGCAGGCAGAGGGGGGACNCCACAGCCAGCTCTGCCCGCGGATGCCACGTCCATTTGCTTCAGCAGGATC
313
PRIMER_INTERNAL_OLIGO_EXPLAIN=considered 1040, too many Ns 27, overlap excluded region 33, low tm 44, high tm 804, ok 132
314
PRIMER_INTERNAL_OLIGO_PENALTY=0.2717
315
PRIMER_INTERNAL_OLIGO_SEQUENCE=CTGCAGGTTTAGAAGGGGTG
316
PRIMER_INTERNAL_OLIGO=64,20
317
PRIMER_INTERNAL_OLIGO_TM=59.728
318
PRIMER_INTERNAL_OLIGO_GC_PERCENT=55.000
319
PRIMER_INTERNAL_OLIGO_SELF_ANY=6.00
320
PRIMER_INTERNAL_OLIGO_SELF_END=0.00
321
PRIMER_INTERNAL_OLIGO_1_PENALTY=0.2717
322
PRIMER_INTERNAL_OLIGO_1_SEQUENCE=CACCCTGCAGGTTTAGAAGG
323
PRIMER_INTERNAL_OLIGO_1=60,20
324
PRIMER_INTERNAL_OLIGO_1_TM=59.728
325
PRIMER_INTERNAL_OLIGO_1_GC_PERCENT=55.000
326
PRIMER_INTERNAL_OLIGO_1_SELF_ANY=8.00
327
PRIMER_INTERNAL_OLIGO_1_SELF_END=0.00
328
PRIMER_INTERNAL_OLIGO_2_PENALTY=0.3125
329
PRIMER_INTERNAL_OLIGO_2_SEQUENCE=AGCACACCCTGCAGGTTTAG
330
PRIMER_INTERNAL_OLIGO_2=56,20
331
PRIMER_INTERNAL_OLIGO_2_TM=60.312
332
PRIMER_INTERNAL_OLIGO_2_GC_PERCENT=55.000
333
PRIMER_INTERNAL_OLIGO_2_SELF_ANY=8.00
334
PRIMER_INTERNAL_OLIGO_2_SELF_END=6.00
335
PRIMER_INTERNAL_OLIGO_3_PENALTY=0.3751
336
PRIMER_INTERNAL_OLIGO_3_SEQUENCE=CATCTGCACCCTGTAAACCC
337
PRIMER_INTERNAL_OLIGO_3=1,20
338
PRIMER_INTERNAL_OLIGO_3_TM=60.375
339
PRIMER_INTERNAL_OLIGO_3_GC_PERCENT=55.000
340
PRIMER_INTERNAL_OLIGO_3_SELF_ANY=4.00
341
PRIMER_INTERNAL_OLIGO_3_SELF_END=0.00
342
PRIMER_INTERNAL_OLIGO_4_PENALTY=0.4079
343
PRIMER_INTERNAL_OLIGO_4_SEQUENCE=TGCAGGTTTAGAAGGGGTGT
344
PRIMER_INTERNAL_OLIGO_4=65,20
345
PRIMER_INTERNAL_OLIGO_4_TM=59.592
346
PRIMER_INTERNAL_OLIGO_4_GC_PERCENT=50.000
347
PRIMER_INTERNAL_OLIGO_4_SELF_ANY=4.00
348
PRIMER_INTERNAL_OLIGO_4_SELF_END=0.00
350
PRIMER_SEQUENCE_ID=left_only
353
PRIMER_TASK=pick_left_only
354
SEQUENCE=TTAGAAGGGGTGTCTGGGTG
355
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
356
PRIMER_LEFT_PENALTY=0.0408
357
PRIMER_LEFT_SEQUENCE=TTAGAAGGGGTGTCTGGGTG
359
PRIMER_LEFT_TM=59.959
360
PRIMER_LEFT_GC_PERCENT=55.000
361
PRIMER_LEFT_SELF_ANY=3.00
362
PRIMER_LEFT_SELF_END=0.00
363
PRIMER_LEFT_END_STABILITY=9.4000
365
PRIMER_SEQUENCE_ID=right_only
366
PRIMER_TASK=pick_left_only
367
SEQUENCE=TTAGAAGGGGTGTCTGGGTG
368
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
369
PRIMER_LEFT_PENALTY=0.0408
370
PRIMER_LEFT_SEQUENCE=TTAGAAGGGGTGTCTGGGTG
372
PRIMER_LEFT_TM=59.959
373
PRIMER_LEFT_GC_PERCENT=55.000
374
PRIMER_LEFT_SELF_ANY=3.00
375
PRIMER_LEFT_SELF_END=0.00
376
PRIMER_LEFT_END_STABILITY=9.4000
378
PRIMER_SEQUENCE_ID=hyb_probe_only
379
PRIMER_INTERNAL_OLIGO_MIN_SIZE=20
380
PRIMER_INTERNAL_OLIGO_MAX_SIZE=20
381
PRIMER_TASK=pick_left_only
382
SEQUENCE=TTAGAAGGGGTGTCTGGGTG
383
PRIMER_LEFT_EXPLAIN=considered 1, ok 1
384
PRIMER_LEFT_PENALTY=0.0408
385
PRIMER_LEFT_SEQUENCE=TTAGAAGGGGTGTCTGGGTG
387
PRIMER_LEFT_TM=59.959
388
PRIMER_LEFT_GC_PERCENT=55.000
389
PRIMER_LEFT_SELF_ANY=3.00
390
PRIMER_LEFT_SELF_END=0.00
391
PRIMER_LEFT_END_STABILITY=9.4000
added
removed
test/primer_task_output
