1
ACCEPTABLE LEFT PRIMERS
2
0-based # self self qual-
3
# sequence start ln N GC% Tm any end lity
4
0 TCGCAATCAAATTTTTTCTATGACC 36 25 0 32.00 62.463 6.00 0.00 7.463
5
1 ATCGCAATCAAATTTTTTCTATGACC 35 26 0 30.77 62.574 6.00 0.00 8.574
6
2 TCGCAATCAAATTTTTTCTATGAC 36 24 0 29.17 59.542 6.00 1.00 4.458
7
3 ATCGCAATCAAATTTTTTCTATGAC 35 25 0 28.00 59.789 6.00 2.00 5.211
8
4 AATCGCAATCAAATTTTTTCTATGAC 34 26 0 26.92 60.843 6.00 1.00 6.843
9
5 GAATCGCAATCAAATTTTTTCTATGAC 33 27 0 29.63 62.235 6.00 1.00 9.235
10
6 TCGCAATCAAATTTTTTCTATGA 36 23 0 26.09 58.793 6.00 2.00 4.207
11
7 ATCGCAATCAAATTTTTTCTATGA 35 24 0 25.00 59.083 6.00 3.00 4.917
12
8 AATCGCAATCAAATTTTTTCTATGA 34 25 0 24.00 60.202 6.00 2.00 5.202
13
9 GAATCGCAATCAAATTTTTTCTATGA 33 26 0 26.92 61.653 6.00 2.00 7.653
14
10 TCGCAATCAAATTTTTTCTATG 36 22 0 27.27 57.077 6.00 2.00 4.923
15
11 ATCGCAATCAAATTTTTTCTATG 35 23 0 26.09 57.456 6.00 2.00 5.544
16
12 AATCGCAATCAAATTTTTTCTATG 34 24 0 25.00 58.677 6.00 2.00 5.323
17
13 GAATCGCAATCAAATTTTTTCTATG 33 25 0 28.00 60.202 6.00 2.00 5.202
18
14 TGAATCGCAATCAAATTTTTTCTATG 32 26 0 26.92 62.397 6.00 2.00 8.397
19
15 ATGAATCGCAATCAAATTTTTTCTATG 31 27 0 25.93 62.507 6.00 2.00 9.507
20
16 GAATCGCAATCAAATTTTTTCTAT 33 24 0 25.00 57.900 6.00 4.00 6.100
21
17 TGAATCGCAATCAAATTTTTTCTAT 32 25 0 24.00 60.202 6.00 4.00 5.202
22
18 ATGAATCGCAATCAAATTTTTTCTAT 31 26 0 23.08 60.408 6.00 4.00 6.408
23
19 TATGAATCGCAATCAAATTTTTTCTAT 30 27 0 22.22 60.404 6.00 4.00 7.404
24
20 GAATCGCAATCAAATTTTTTCTA 33 23 0 26.09 57.561 6.00 2.00 5.439
25
21 TGAATCGCAATCAAATTTTTTCTA 32 24 0 25.00 59.977 6.00 2.00 4.023
26
22 ATGAATCGCAATCAAATTTTTTCTA 31 25 0 24.00 60.202 6.00 2.00 5.202
27
23 TATGAATCGCAATCAAATTTTTTCTA 30 26 0 23.08 60.204 6.00 2.00 6.204
28
24 TTATGAATCGCAATCAAATTTTTTCTA 29 27 0 22.22 61.199 6.00 2.00 8.199
29
25 GAATCGCAATCAAATTTTTTCT 33 22 0 27.27 57.467 6.00 2.00 4.533
30
26 TGAATCGCAATCAAATTTTTTCT 32 23 0 26.09 59.967 6.00 2.00 3.033
31
27 ATGAATCGCAATCAAATTTTTTCT 31 24 0 25.00 60.199 6.00 2.00 4.199
32
28 TATGAATCGCAATCAAATTTTTTCT 30 25 0 24.00 60.202 6.00 2.00 5.202
33
29 TTATGAATCGCAATCAAATTTTTTCT 29 26 0 23.08 61.226 6.00 2.00 7.226
34
30 ATTATGAATCGCAATCAAATTTTTTCT 28 27 0 22.22 61.382 6.00 2.00 8.382
35
31 TGAATCGCAATCAAATTTTTTC 32 22 0 27.27 59.091 6.00 2.00 2.909
36
32 ATGAATCGCAATCAAATTTTTTC 31 23 0 26.09 59.374 6.00 2.00 3.626
37
33 TATGAATCGCAATCAAATTTTTTC 30 24 0 25.00 59.404 6.00 2.00 4.596
38
34 TTATGAATCGCAATCAAATTTTTTC 29 25 0 24.00 60.502 6.00 2.00 5.502
39
35 ATTATGAATCGCAATCAAATTTTTTC 28 26 0 23.08 60.694 6.00 2.00 6.694
40
36 AATTATGAATCGCAATCAAATTTTTTC 27 27 0 22.22 61.652 8.00 2.00 8.652
41
37 TGAATCGCAATCAAATTTTTT 32 21 0 23.81 57.334 6.00 4.00 3.666
42
38 ATGAATCGCAATCAAATTTTTT 31 22 0 22.73 57.710 6.00 4.00 4.290
43
39 TATGAATCGCAATCAAATTTTTT 30 23 0 21.74 57.795 6.00 4.00 5.205
44
40 TTATGAATCGCAATCAAATTTTTT 29 24 0 20.83 58.994 6.00 4.00 5.006
45
41 ATTATGAATCGCAATCAAATTTTTT 28 25 0 20.00 59.255 6.00 4.00 5.745
46
42 TTATGAATCGCAATCAAATTTTT 29 23 0 21.74 57.795 6.00 4.00 5.205
47
43 ATTATGAATCGCAATCAAATTTTT 28 24 0 20.83 58.122 6.00 4.00 5.878
48
44 AATTATGAATCGCAATCAAATTTTT 27 25 0 20.00 59.255 8.00 4.00 5.745
49
45 AATTATGAATCGCAATCAAATTTT 27 24 0 20.83 58.122 8.00 5.00 5.878
added
removed
test/primer_list_last/SP01-06.txt.for
