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- Committer: Bazaar Package Importer
- Author(s): Charles Plessy
- Date: 2006-09-28 20:18:54 UTC
- Revision ID: james.westby@ubuntu.com-20060928201854-45pwapz5e3a6d684
Tags: upstream-1.0b
Import upstream version 1.0b
- files added:
- src
- test
- test/primer1_list_last
- test/primer1_list_tmp
- test/primer_global_err
- test/primer_list_last
- test/primer_list_tmp
added
removed
test/primer_num_best_output
1
PRIMER_NUM_RETURN=2
2
PRIMER_OPT_SIZE=20
3
PRIMER_MAX_POLY_X=100
4
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
5
PRIMER_PRODUCT_SIZE_RANGE=125-175 75-1000
6
PRIMER_MAX_GC=80.0
7
PRIMER_MAX_SIZE=25
8
PRIMER_MAX_TM=63.0
9
PRIMER_MIN_SIZE=18
10
PRIMER_MIN_GC=20
11
PRIMER_MIN_TM=57.0
12
PRIMER_OPT_TM=60.0
13
PRIMER_SELF_ANY=12
14
PRIMER_SELF_END=6
15
MARKER_NAME=01.RHAP33FA1.seq
16
SEQUENCE=NNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGGAAACAGTATAAATAAGCAAAGATATTTGAATCCTGTCACCTTTTCATCCTCTGTCCTTTTGCCCAAGATAACATTGTTATAGTTGCCTTTACCCTGCACTGTACAACTCCTACTAAACTTCTTAGGTAGGAGGTGTGTTGGCTTTTCCTTGNGGGNTGGGTAGTCACAAAGTGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAANTAATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
17
INCLUDED_REGION=20,315
18
TARGET=81,30,CA
19
PRIMER_PAIR_PENALTY=7.7339
20
PRIMER_LEFT_PENALTY=7.538496
21
PRIMER_RIGHT_PENALTY=0.195358
22
PRIMER_LEFT_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA
23
PRIMER_RIGHT_SEQUENCE=GGCAAAAGGACAGAGGATGA
24
PRIMER_LEFT=20,25
25
PRIMER_RIGHT=177,20
26
PRIMER_LEFT_TM=57.462
27
PRIMER_RIGHT_TM=60.195
28
PRIMER_LEFT_GC_PERCENT=32.000
29
PRIMER_RIGHT_GC_PERCENT=50.000
30
PRIMER_LEFT_SELF_ANY=5.00
31
PRIMER_RIGHT_SELF_ANY=2.00
32
PRIMER_LEFT_SELF_END=0.00
33
PRIMER_RIGHT_SELF_END=1.00
34
PRIMER_LEFT_END_STABILITY=6.0000
35
PRIMER_RIGHT_END_STABILITY=6.6000
36
PRIMER_PAIR_COMPL_ANY=4.00
37
PRIMER_PAIR_COMPL_END=0.00
38
PRIMER_PRODUCT_SIZE=158
39
PRIMER_PAIR_PENALTY_1=7.8680
40
PRIMER_LEFT_1_PENALTY=7.538496
41
PRIMER_RIGHT_1_PENALTY=0.329550
42
PRIMER_LEFT_1_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA
43
PRIMER_RIGHT_1_SEQUENCE=ATCTTGGGCAAAAGGACAGA
44
PRIMER_LEFT_1=20,25
45
PRIMER_RIGHT_1=183,20
46
PRIMER_LEFT_1_TM=57.462
47
PRIMER_RIGHT_1_TM=59.670
48
PRIMER_LEFT_1_GC_PERCENT=32.000
49
PRIMER_RIGHT_1_GC_PERCENT=45.000
50
PRIMER_LEFT_1_SELF_ANY=5.00
51
PRIMER_RIGHT_1_SELF_ANY=4.00
52
PRIMER_LEFT_1_SELF_END=0.00
53
PRIMER_RIGHT_1_SELF_END=0.00
54
PRIMER_LEFT_1_END_STABILITY=6.0000
55
PRIMER_RIGHT_1_END_STABILITY=6.4000
56
PRIMER_PAIR_1_COMPL_ANY=4.00
57
PRIMER_PAIR_1_COMPL_END=0.00
58
PRIMER_PRODUCT_SIZE_1=164
59
=
60
MARKER_NAME=01.RHAP34FA1.seq
61
SEQUENCE=NNNNNNNNNNNNNNNNNNNACTTGTCATTTCTCAGAGACATCTATACCTCAGAGACAAACTTCTCAGAAAACAACCCTAAGGTTGAACAAAACATGGCTGTATGTATCTATATTATTACACGCGCGTGGGNNANNNNNNNNNNNNNNNNCGTTGATTTTTTATACTAACAAGANNATGTCCATATAACATGCTAGTATATCATACACACATACNNNNNNNNNNNNNNNNTTATACNNNNNNNNNNNNNNNNAGGAATGTGTGTGCNACCTNATGNTTAACNNCAAGTGTACCCNNGAATNTGTNGNTGNTGGCNT
62
INCLUDED_REGION=19,296
63
TARGET=134,15,CA
64
TARGET=213,16,AT
65
TARGET=235,16,AT
66
=
67
PRIMER_NUM_RETURN=1
68
MARKER_NAME=03.RHAP34FA3.seq
69
SEQUENCE=TNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGCAGGAGGATGAAATGCAATCTGAAGAGAGCCCCTGAGCCAGTCCAGCCTCCCCATCATGGCCTTTGCCTTCCAACCCCTGCGTTCTTCCGGGCCTCTGTGAAATGCTATCTTGATAATGTCTGTAATCACTTGATTGCAGCCAAGCTAAAGGCTGGCCCTGACTGGGATGCAGAATGAAGAGTTGTGGGGAGGCGAGGGATAGCAGGAACGAACCCAAAAAACACACTGCCCTTTCACACAGTCAT
70
INCLUDED_REGION=0,278
71
TARGET=1,28,CA
72
=
73
MARKER_NAME=03.RHAP34FE3.seq
74
SEQUENCE=NNNNNNNNNNNNNNNNNTCATACAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCATATTATGTATATGCTGCATCTAACTTTATCCTTTCAGACAGCCTAGCTGCAGCTCAGGATGCTGTCTATTCCAGACTTACCTCAAACATGCAGCAGCAGCAGTACATGAAGAAATTGAATTATTCCATGTTAAGGCAAAGATAAACACTATCCACAAATATAGGCTTATTAGCNAGGTCTAAAGAAAAATTTCACTCACTAAGCACTCAA
75
INCLUDED_REGION=17,284
76
TARGET=27,60,AC
77
=
78
MARKER_NAME=04.RHAP33FE4.seq
79
SEQUENCE=NNNNNNNNNNNNNNNNNNTCCCAGAATGCAATGCCCAGAAAAGTCTCGCCCTCAATCTCATAGTTTCACTCAGCGTCTAGCACCGAGGAAAGTTTTGTGAGAAGGGAGGAGTAAGTACTGGAGAGAGAAGAGGAATTAAACAATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTGTGNNNNNNNNNNNNNNNNNNNNNNTGTGTGTGTGTATAGGTATACCGNATATGTGTGTGGTGTATAGGCATAGGTATATGTATATGTGCNNNNNNNNNNNNNGNNNNNNNNNNNNNNNCAGANAGAGAGACNANACCNCNCNNGGNGNNNCNNNNGNGCNNAGAGNGNGNCNGANNGNGAGNGACCAC
80
INCLUDED_REGION=18,367
81
TARGET=146,46,AT
82
TARGET=199,22,TA
83
TARGET=286,13,GT
84
TARGET=300,15,AG
85
PRIMER_PAIR_PENALTY=7.3535
86
PRIMER_LEFT_PENALTY=4.174047
87
PRIMER_RIGHT_PENALTY=3.179421
88
PRIMER_LEFT_SEQUENCE=TGAGAAGGGAGGAGTAAGTACTGG
89
PRIMER_RIGHT_SEQUENCE=CCTATGCCTATACACCACACACA
90
PRIMER_LEFT=97,24
91
PRIMER_RIGHT=270,23
92
PRIMER_LEFT_TM=60.174
93
PRIMER_RIGHT_TM=59.821
94
PRIMER_LEFT_GC_PERCENT=50.000
95
PRIMER_RIGHT_GC_PERCENT=47.826
96
PRIMER_LEFT_SELF_ANY=6.00
97
PRIMER_RIGHT_SELF_ANY=4.00
98
PRIMER_LEFT_SELF_END=2.00
99
PRIMER_RIGHT_SELF_END=0.00
100
PRIMER_LEFT_END_STABILITY=7.9000
101
PRIMER_RIGHT_END_STABILITY=6.4000
102
PRIMER_PAIR_COMPL_ANY=4.00
103
PRIMER_PAIR_COMPL_END=1.00
104
PRIMER_PRODUCT_SIZE=174
105
=
106
MARKER_NAME=05.RHAP33FA5.seq
107
SEQUENCE=NNNNNNNNNNCCTAATACTGTGAAGACAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGGAGGTGTTCCAATAGAGAAGCAAGATCATGACACCCTCTGACCTTTGCCCCTGGCAACCATTTTGGGTATGTTCTCATGGCTCCCTCCACCCCCAGCCTCAGTTTCCCTGAGAGGGAAGGGGGATATAGGGAGAGGAGAGCCGNGCCCCGCCCCCATCCGGGATCANTGTTGGGGGAAGAATGNGTGCAAGCCAGGCCACCNGGGGTTAANGCGGTGACCTCAGCCGNGCGCCCCAGGAGGGGTNNNAGANCCTCNANTTCGTANTCNNTGAGATCAATAANAATGTTTCAATGTGTGAAA
108
INCLUDED_REGION=10,369
109
TARGET=30,44,GT
110
=
111
MARKER_NAME=05.RHAP33FE5.seq
112
SEQUENCE=NNNNNNNNNNNNNNCTTCAGTAANAGATTCTTATCACAGGAAAGAAGGTGGAGAATGATAAACAAGGAGTTCAACATCAACCTCGGGCCTGTATATGTATGAAGTACGTACACCCAAATCCTATACATACATGTTCGTGCAAACACTCAAATACTACATATACTTGTATATACAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTATACAAACACTTATTTATTGGTTTTATTTTGCCTTAAAGGATTAAGTTGTGATTACTAATACATTTTATTTTGCTTTTGGAGACAGGGTCTTGCTAGGTATCCTAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
113
INCLUDED_REGION=14,306
114
TARGET=176,34,CA
115
PRIMER_PAIR_PENALTY=0.8585
116
PRIMER_LEFT_PENALTY=0.702456
117
PRIMER_RIGHT_PENALTY=0.156061
118
PRIMER_LEFT_SEQUENCE=CATGTTCGTGCAAACACTCA
119
PRIMER_RIGHT_SEQUENCE=AGACCCTGTCTCCAAAAGCA
120
PRIMER_LEFT=129,20
121
PRIMER_RIGHT=302,20
122
PRIMER_LEFT_TM=59.298
123
PRIMER_RIGHT_TM=59.844
124
PRIMER_LEFT_GC_PERCENT=45.000
125
PRIMER_RIGHT_GC_PERCENT=50.000
126
PRIMER_LEFT_SELF_ANY=6.00
127
PRIMER_RIGHT_SELF_ANY=5.00
128
PRIMER_LEFT_SELF_END=1.00
129
PRIMER_RIGHT_SELF_END=0.00
130
PRIMER_LEFT_END_STABILITY=6.4000
131
PRIMER_RIGHT_END_STABILITY=8.5000
132
PRIMER_PAIR_COMPL_ANY=4.00
133
PRIMER_PAIR_COMPL_END=0.00
134
PRIMER_PRODUCT_SIZE=174
135
=
136
PRIMER_NUM_RETURN=40
137
MARKER_NAME=05.RHAP34FA5.seq
138
SEQUENCE=NNNNNNNNNACCTAACTAAAACAGACCATAAACTGAGAACCTAGTATTCATGCAGCTGAGACTTTGAAGGCCACTCTCATTCAGCCAGGTGTGTGAGGANCGCGCGGCCGACATCTACTACTACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNACAACNNNNNNNNNNNNNNNNCACAAAGNNNNNNNNNNNNNNGTAGGACAGACAGAGATTAGACAGAGTAGTAAGGGGAGAGANNAGGTTGGTTGTGGGAAGGACCTTATTACTTCACAA
139
INCLUDED_REGION=9,297
140
TARGET=125,36,AC
141
TARGET=162,24,GA
142
TARGET=191,16,AG
143
TARGET=214,14,GA
144
PRIMER_PAIR_PENALTY=9.8843
145
PRIMER_LEFT_PENALTY=1.973952
146
PRIMER_RIGHT_PENALTY=7.910390
147
PRIMER_LEFT_SEQUENCE=GCGGCCGACATCTACTACTA
148
PRIMER_RIGHT_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
149
PRIMER_LEFT=103,20
150
PRIMER_RIGHT=268,25
151
PRIMER_LEFT_TM=58.026
152
PRIMER_RIGHT_TM=57.090
153
PRIMER_LEFT_GC_PERCENT=55.000
154
PRIMER_RIGHT_GC_PERCENT=44.000
155
PRIMER_LEFT_SELF_ANY=6.00
156
PRIMER_RIGHT_SELF_ANY=3.00
157
PRIMER_LEFT_SELF_END=4.00
158
PRIMER_RIGHT_SELF_END=1.00
159
PRIMER_LEFT_END_STABILITY=4.7000
160
PRIMER_RIGHT_END_STABILITY=6.0000
161
PRIMER_PAIR_COMPL_ANY=4.00
162
PRIMER_PAIR_COMPL_END=1.00
163
PRIMER_PRODUCT_SIZE=166
164
PRIMER_PAIR_PENALTY_1=10.0123
165
PRIMER_LEFT_1_PENALTY=2.101866
166
PRIMER_RIGHT_1_PENALTY=7.910390
167
PRIMER_LEFT_1_SEQUENCE=GCGGCCGACATCTACTACTAC
168
PRIMER_RIGHT_1_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
169
PRIMER_LEFT_1=103,21
170
PRIMER_RIGHT_1=268,25
171
PRIMER_LEFT_1_TM=58.898
172
PRIMER_RIGHT_1_TM=57.090
173
PRIMER_LEFT_1_GC_PERCENT=57.143
174
PRIMER_RIGHT_1_GC_PERCENT=44.000
175
PRIMER_LEFT_1_SELF_ANY=6.00
176
PRIMER_RIGHT_1_SELF_ANY=3.00
177
PRIMER_LEFT_1_SELF_END=2.00
178
PRIMER_RIGHT_1_SELF_END=1.00
179
PRIMER_LEFT_1_END_STABILITY=5.1000
180
PRIMER_RIGHT_1_END_STABILITY=6.0000
181
PRIMER_PAIR_1_COMPL_ANY=4.00
182
PRIMER_PAIR_1_COMPL_END=0.00
183
PRIMER_PRODUCT_SIZE_1=166
184
PRIMER_PAIR_PENALTY_2=10.0792
185
PRIMER_LEFT_2_PENALTY=2.168831
186
PRIMER_RIGHT_2_PENALTY=7.910390
187
PRIMER_LEFT_2_SEQUENCE=GCGGCCGACATCTACTACTACT
188
PRIMER_RIGHT_2_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
189
PRIMER_LEFT_2=103,22
190
PRIMER_RIGHT_2=268,25
191
PRIMER_LEFT_2_TM=59.831
192
PRIMER_RIGHT_2_TM=57.090
193
PRIMER_LEFT_2_GC_PERCENT=54.545
194
PRIMER_RIGHT_2_GC_PERCENT=44.000
195
PRIMER_LEFT_2_SELF_ANY=6.00
196
PRIMER_RIGHT_2_SELF_ANY=3.00
197
PRIMER_LEFT_2_SELF_END=3.00
198
PRIMER_RIGHT_2_SELF_END=1.00
199
PRIMER_LEFT_2_END_STABILITY=5.4000
200
PRIMER_RIGHT_2_END_STABILITY=6.0000
201
PRIMER_PAIR_2_COMPL_ANY=4.00
202
PRIMER_PAIR_2_COMPL_END=1.00
203
PRIMER_PRODUCT_SIZE_2=166
204
PRIMER_PAIR_PENALTY_3=10.5142
205
PRIMER_LEFT_3_PENALTY=2.603822
206
PRIMER_RIGHT_3_PENALTY=7.910390
207
PRIMER_LEFT_3_SEQUENCE=CGCGGCCGACATCTACTACT
208
PRIMER_RIGHT_3_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
209
PRIMER_LEFT_3=102,20
210
PRIMER_RIGHT_3=268,25
211
PRIMER_LEFT_3_TM=62.604
212
PRIMER_RIGHT_3_TM=57.090
213
PRIMER_LEFT_3_GC_PERCENT=60.000
214
PRIMER_RIGHT_3_GC_PERCENT=44.000
215
PRIMER_LEFT_3_SELF_ANY=6.00
216
PRIMER_RIGHT_3_SELF_ANY=3.00
217
PRIMER_LEFT_3_SELF_END=2.00
218
PRIMER_RIGHT_3_SELF_END=1.00
219
PRIMER_LEFT_3_END_STABILITY=5.4000
220
PRIMER_RIGHT_3_END_STABILITY=6.0000
221
PRIMER_PAIR_3_COMPL_ANY=4.00
222
PRIMER_PAIR_3_COMPL_END=1.00
223
PRIMER_PRODUCT_SIZE_3=167
224
PRIMER_PAIR_PENALTY_4=10.6566
225
PRIMER_LEFT_4_PENALTY=2.746227
226
PRIMER_RIGHT_4_PENALTY=7.910390
227
PRIMER_LEFT_4_SEQUENCE=CGCGGCCGACATCTACTAC
228
PRIMER_RIGHT_4_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
229
PRIMER_LEFT_4=102,19
230
PRIMER_RIGHT_4=268,25
231
PRIMER_LEFT_4_TM=61.746
232
PRIMER_RIGHT_4_TM=57.090
233
PRIMER_LEFT_4_GC_PERCENT=63.158
234
PRIMER_RIGHT_4_GC_PERCENT=44.000
235
PRIMER_LEFT_4_SELF_ANY=6.00
236
PRIMER_RIGHT_4_SELF_ANY=3.00
237
PRIMER_LEFT_4_SELF_END=1.00
238
PRIMER_RIGHT_4_SELF_END=1.00
239
PRIMER_LEFT_4_END_STABILITY=5.1000
240
PRIMER_RIGHT_4_END_STABILITY=6.0000
241
PRIMER_PAIR_4_COMPL_ANY=4.00
242
PRIMER_PAIR_4_COMPL_END=0.00
243
PRIMER_PRODUCT_SIZE_4=167
244
PRIMER_PAIR_PENALTY_5=10.8467
245
PRIMER_LEFT_5_PENALTY=2.936346
246
PRIMER_RIGHT_5_PENALTY=7.910390
247
PRIMER_LEFT_5_SEQUENCE=CGCGGCCGACATCTACTA
248
PRIMER_RIGHT_5_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
249
PRIMER_LEFT_5=102,18
250
PRIMER_RIGHT_5=268,25
251
PRIMER_LEFT_5_TM=60.936
252
PRIMER_RIGHT_5_TM=57.090
253
PRIMER_LEFT_5_GC_PERCENT=61.111
254
PRIMER_RIGHT_5_GC_PERCENT=44.000
255
PRIMER_LEFT_5_SELF_ANY=6.00
256
PRIMER_RIGHT_5_SELF_ANY=3.00
257
PRIMER_LEFT_5_SELF_END=3.00
258
PRIMER_RIGHT_5_SELF_END=1.00
259
PRIMER_LEFT_5_END_STABILITY=4.7000
260
PRIMER_RIGHT_5_END_STABILITY=6.0000
261
PRIMER_PAIR_5_COMPL_ANY=4.00
262
PRIMER_PAIR_5_COMPL_END=1.00
263
PRIMER_PRODUCT_SIZE_5=167
264
PRIMER_PAIR_PENALTY_6=10.9749
265
PRIMER_LEFT_6_PENALTY=3.064519
266
PRIMER_RIGHT_6_PENALTY=7.910390
267
PRIMER_LEFT_6_SEQUENCE=GCGGCCGACATCTACTACT
268
PRIMER_RIGHT_6_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
269
PRIMER_LEFT_6=103,19
270
PRIMER_RIGHT_6=268,25
271
PRIMER_LEFT_6_TM=57.935
272
PRIMER_RIGHT_6_TM=57.090
273
PRIMER_LEFT_6_GC_PERCENT=57.895
274
PRIMER_RIGHT_6_GC_PERCENT=44.000
275
PRIMER_LEFT_6_SELF_ANY=6.00
276
PRIMER_RIGHT_6_SELF_ANY=3.00
277
PRIMER_LEFT_6_SELF_END=2.00
278
PRIMER_RIGHT_6_SELF_END=1.00
279
PRIMER_LEFT_6_END_STABILITY=5.4000
280
PRIMER_RIGHT_6_END_STABILITY=6.0000
281
PRIMER_PAIR_6_COMPL_ANY=4.00
282
PRIMER_PAIR_6_COMPL_END=1.00
283
PRIMER_PRODUCT_SIZE_6=166
284
PRIMER_PAIR_PENALTY_7=11.4300
285
PRIMER_LEFT_7_PENALTY=3.519592
286
PRIMER_RIGHT_7_PENALTY=7.910390
287
PRIMER_LEFT_7_SEQUENCE=CGCGGCCGACATCTACTACTA
288
PRIMER_RIGHT_7_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
289
PRIMER_LEFT_7=102,21
290
PRIMER_RIGHT_7=268,25
291
PRIMER_LEFT_7_TM=62.520
292
PRIMER_RIGHT_7_TM=57.090
293
PRIMER_LEFT_7_GC_PERCENT=57.143
294
PRIMER_RIGHT_7_GC_PERCENT=44.000
295
PRIMER_LEFT_7_SELF_ANY=6.00
296
PRIMER_RIGHT_7_SELF_ANY=3.00
297
PRIMER_LEFT_7_SELF_END=4.00
298
PRIMER_RIGHT_7_SELF_END=1.00
299
PRIMER_LEFT_7_END_STABILITY=4.7000
300
PRIMER_RIGHT_7_END_STABILITY=6.0000
301
PRIMER_PAIR_7_COMPL_ANY=4.00
302
PRIMER_PAIR_7_COMPL_END=1.00
303
PRIMER_PRODUCT_SIZE_7=167
304
PRIMER_PAIR_PENALTY_8=0.3479
305
PRIMER_LEFT_8_PENALTY=0.003322
306
PRIMER_RIGHT_8_PENALTY=0.344549
307
PRIMER_LEFT_8_SEQUENCE=GCTGAGACTTTGAAGGCCAC
308
PRIMER_RIGHT_8_SEQUENCE=TAAGGTCCTTCCCACAACCA
309
PRIMER_LEFT_8=54,20
310
PRIMER_RIGHT_8=294,20
311
PRIMER_LEFT_8_TM=59.997
312
PRIMER_RIGHT_8_TM=60.345
313
PRIMER_LEFT_8_GC_PERCENT=55.000
314
PRIMER_RIGHT_8_GC_PERCENT=50.000
315
PRIMER_LEFT_8_SELF_ANY=4.00
316
PRIMER_RIGHT_8_SELF_ANY=7.00
317
PRIMER_LEFT_8_SELF_END=3.00
318
PRIMER_RIGHT_8_SELF_END=0.00
319
PRIMER_LEFT_8_END_STABILITY=9.4000
320
PRIMER_RIGHT_8_END_STABILITY=8.2000
321
PRIMER_PAIR_8_COMPL_ANY=5.00
322
PRIMER_PAIR_8_COMPL_END=1.00
323
PRIMER_PRODUCT_SIZE_8=241
324
PRIMER_PAIR_PENALTY_9=0.3873
325
PRIMER_LEFT_9_PENALTY=0.042732
326
PRIMER_RIGHT_9_PENALTY=0.344549
327
PRIMER_LEFT_9_SEQUENCE=GAAGGCCACTCTCATTCAGC
328
PRIMER_RIGHT_9_SEQUENCE=TAAGGTCCTTCCCACAACCA
329
PRIMER_LEFT_9=65,20
330
PRIMER_RIGHT_9=294,20
331
PRIMER_LEFT_9_TM=59.957
332
PRIMER_RIGHT_9_TM=60.345
333
PRIMER_LEFT_9_GC_PERCENT=55.000
334
PRIMER_RIGHT_9_GC_PERCENT=50.000
335
PRIMER_LEFT_9_SELF_ANY=4.00
336
PRIMER_RIGHT_9_SELF_ANY=7.00
337
PRIMER_LEFT_9_SELF_END=2.00
338
PRIMER_RIGHT_9_SELF_END=0.00
339
PRIMER_LEFT_9_END_STABILITY=8.2000
340
PRIMER_RIGHT_9_END_STABILITY=8.2000
341
PRIMER_PAIR_9_COMPL_ANY=5.00
342
PRIMER_PAIR_9_COMPL_END=0.00
343
PRIMER_PRODUCT_SIZE_9=230
344
PRIMER_PAIR_PENALTY_10=0.4783
345
PRIMER_LEFT_10_PENALTY=0.133741
346
PRIMER_RIGHT_10_PENALTY=0.344549
347
PRIMER_LEFT_10_SEQUENCE=AGCTGAGACTTTGAAGGCCA
348
PRIMER_RIGHT_10_SEQUENCE=TAAGGTCCTTCCCACAACCA
349
PRIMER_LEFT_10=53,20
350
PRIMER_RIGHT_10=294,20
351
PRIMER_LEFT_10_TM=60.134
352
PRIMER_RIGHT_10_TM=60.345
353
PRIMER_LEFT_10_GC_PERCENT=50.000
354
PRIMER_RIGHT_10_GC_PERCENT=50.000
355
PRIMER_LEFT_10_SELF_ANY=4.00
356
PRIMER_RIGHT_10_SELF_ANY=7.00
357
PRIMER_LEFT_10_SELF_END=4.00
358
PRIMER_RIGHT_10_SELF_END=0.00
359
PRIMER_LEFT_10_END_STABILITY=11.2000
360
PRIMER_RIGHT_10_END_STABILITY=8.2000
361
PRIMER_PAIR_10_COMPL_ANY=5.00
362
PRIMER_PAIR_10_COMPL_END=1.00
363
PRIMER_PRODUCT_SIZE_10=242
364
PRIMER_PAIR_PENALTY_11=0.4783
365
PRIMER_LEFT_11_PENALTY=0.133741
366
PRIMER_RIGHT_11_PENALTY=0.344549
367
PRIMER_LEFT_11_SEQUENCE=CAGCTGAGACTTTGAAGGCC
368
PRIMER_RIGHT_11_SEQUENCE=TAAGGTCCTTCCCACAACCA
369
PRIMER_LEFT_11=52,20
370
PRIMER_RIGHT_11=294,20
371
PRIMER_LEFT_11_TM=60.134
372
PRIMER_RIGHT_11_TM=60.345
373
PRIMER_LEFT_11_GC_PERCENT=55.000
374
PRIMER_RIGHT_11_GC_PERCENT=50.000
375
PRIMER_LEFT_11_SELF_ANY=6.00
376
PRIMER_RIGHT_11_SELF_ANY=7.00
377
PRIMER_LEFT_11_SELF_END=4.00
378
PRIMER_RIGHT_11_SELF_END=0.00
379
PRIMER_LEFT_11_END_STABILITY=10.9000
380
PRIMER_RIGHT_11_END_STABILITY=8.2000
381
PRIMER_PAIR_11_COMPL_ANY=5.00
382
PRIMER_PAIR_11_COMPL_END=2.00
383
PRIMER_PRODUCT_SIZE_11=243
384
PRIMER_PAIR_PENALTY_12=0.6053
385
PRIMER_LEFT_12_PENALTY=0.260757
386
PRIMER_RIGHT_12_PENALTY=0.344549
387
PRIMER_LEFT_12_SEQUENCE=CCACTCTCATTCAGCCAGGT
388
PRIMER_RIGHT_12_SEQUENCE=TAAGGTCCTTCCCACAACCA
389
PRIMER_LEFT_12=70,20
390
PRIMER_RIGHT_12=294,20
391
PRIMER_LEFT_12_TM=60.261
392
PRIMER_RIGHT_12_TM=60.345
393
PRIMER_LEFT_12_GC_PERCENT=55.000
394
PRIMER_RIGHT_12_GC_PERCENT=50.000
395
PRIMER_LEFT_12_SELF_ANY=3.00
396
PRIMER_RIGHT_12_SELF_ANY=7.00
397
PRIMER_LEFT_12_SELF_END=1.00
398
PRIMER_RIGHT_12_SELF_END=0.00
399
PRIMER_LEFT_12_END_STABILITY=7.9000
400
PRIMER_RIGHT_12_END_STABILITY=8.2000
401
PRIMER_PAIR_12_COMPL_ANY=3.00
402
PRIMER_PAIR_12_COMPL_END=2.00
403
PRIMER_PRODUCT_SIZE_12=225
404
PRIMER_PAIR_PENALTY_13=0.6297
405
PRIMER_LEFT_13_PENALTY=0.285121
406
PRIMER_RIGHT_13_PENALTY=0.344549
407
PRIMER_LEFT_13_SEQUENCE=GCAGCTGAGACTTTGAAGGC
408
PRIMER_RIGHT_13_SEQUENCE=TAAGGTCCTTCCCACAACCA
409
PRIMER_LEFT_13=51,20
410
PRIMER_RIGHT_13=294,20
411
PRIMER_LEFT_13_TM=60.285
412
PRIMER_RIGHT_13_TM=60.345
413
PRIMER_LEFT_13_GC_PERCENT=55.000
414
PRIMER_RIGHT_13_GC_PERCENT=50.000
415
PRIMER_LEFT_13_SELF_ANY=6.00
416
PRIMER_RIGHT_13_SELF_ANY=7.00
417
PRIMER_LEFT_13_SELF_END=4.00
418
PRIMER_RIGHT_13_SELF_END=0.00
419
PRIMER_LEFT_13_END_STABILITY=9.7000
420
PRIMER_RIGHT_13_END_STABILITY=8.2000
421
PRIMER_PAIR_13_COMPL_ANY=5.00
422
PRIMER_PAIR_13_COMPL_END=1.00
423
PRIMER_PRODUCT_SIZE_13=244
424
PRIMER_PAIR_PENALTY_14=0.6540
425
PRIMER_LEFT_14_PENALTY=0.309402
426
PRIMER_RIGHT_14_PENALTY=0.344549
427
PRIMER_LEFT_14_SEQUENCE=CATTCAGCCAGGTGTGTGAG
428
PRIMER_RIGHT_14_SEQUENCE=TAAGGTCCTTCCCACAACCA
429
PRIMER_LEFT_14=77,20
430
PRIMER_RIGHT_14=294,20
431
PRIMER_LEFT_14_TM=60.309
432
PRIMER_RIGHT_14_TM=60.345
433
PRIMER_LEFT_14_GC_PERCENT=55.000
434
PRIMER_RIGHT_14_GC_PERCENT=50.000
435
PRIMER_LEFT_14_SELF_ANY=4.00
436
PRIMER_RIGHT_14_SELF_ANY=7.00
437
PRIMER_LEFT_14_SELF_END=2.00
438
PRIMER_RIGHT_14_SELF_END=0.00
439
PRIMER_LEFT_14_END_STABILITY=6.4000
440
PRIMER_RIGHT_14_END_STABILITY=8.2000
441
PRIMER_PAIR_14_COMPL_ANY=5.00
442
PRIMER_PAIR_14_COMPL_END=4.00
443
PRIMER_PRODUCT_SIZE_14=218
444
PRIMER_PAIR_PENALTY_15=0.6540
445
PRIMER_LEFT_15_PENALTY=0.309402
446
PRIMER_RIGHT_15_PENALTY=0.344549
447
PRIMER_LEFT_15_SEQUENCE=CTCATTCAGCCAGGTGTGTG
448
PRIMER_RIGHT_15_SEQUENCE=TAAGGTCCTTCCCACAACCA
449
PRIMER_LEFT_15=75,20
450
PRIMER_RIGHT_15=294,20
451
PRIMER_LEFT_15_TM=60.309
452
PRIMER_RIGHT_15_TM=60.345
453
PRIMER_LEFT_15_GC_PERCENT=55.000
454
PRIMER_RIGHT_15_GC_PERCENT=50.000
455
PRIMER_LEFT_15_SELF_ANY=3.00
456
PRIMER_RIGHT_15_SELF_ANY=7.00
457
PRIMER_LEFT_15_SELF_END=3.00
458
PRIMER_RIGHT_15_SELF_END=0.00
459
PRIMER_LEFT_15_END_STABILITY=6.4000
460
PRIMER_RIGHT_15_END_STABILITY=8.2000
461
PRIMER_PAIR_15_COMPL_ANY=5.00
462
PRIMER_PAIR_15_COMPL_END=4.00
463
PRIMER_PRODUCT_SIZE_15=220
464
PRIMER_PAIR_PENALTY_16=0.9299
465
PRIMER_LEFT_16_PENALTY=0.585389
466
PRIMER_RIGHT_16_PENALTY=0.344549
467
PRIMER_LEFT_16_SEQUENCE=CTCTCATTCAGCCAGGTGTG
468
PRIMER_RIGHT_16_SEQUENCE=TAAGGTCCTTCCCACAACCA
469
PRIMER_LEFT_16=73,20
470
PRIMER_RIGHT_16=294,20
471
PRIMER_LEFT_16_TM=59.415
472
PRIMER_RIGHT_16_TM=60.345
473
PRIMER_LEFT_16_GC_PERCENT=55.000
474
PRIMER_RIGHT_16_GC_PERCENT=50.000
475
PRIMER_LEFT_16_SELF_ANY=3.00
476
PRIMER_RIGHT_16_SELF_ANY=7.00
477
PRIMER_LEFT_16_SELF_END=1.00
478
PRIMER_RIGHT_16_SELF_END=0.00
479
PRIMER_LEFT_16_END_STABILITY=6.4000
480
PRIMER_RIGHT_16_END_STABILITY=8.2000
481
PRIMER_PAIR_16_COMPL_ANY=4.00
482
PRIMER_PAIR_16_COMPL_END=3.00
483
PRIMER_PRODUCT_SIZE_16=222
484
PRIMER_PAIR_PENALTY_17=0.9299
485
PRIMER_LEFT_17_PENALTY=0.585389
486
PRIMER_RIGHT_17_PENALTY=0.344549
487
PRIMER_LEFT_17_SEQUENCE=CACTCTCATTCAGCCAGGTG
488
PRIMER_RIGHT_17_SEQUENCE=TAAGGTCCTTCCCACAACCA
489
PRIMER_LEFT_17=71,20
490
PRIMER_RIGHT_17=294,20
491
PRIMER_LEFT_17_TM=59.415
492
PRIMER_RIGHT_17_TM=60.345
493
PRIMER_LEFT_17_GC_PERCENT=55.000
494
PRIMER_RIGHT_17_GC_PERCENT=50.000
495
PRIMER_LEFT_17_SELF_ANY=3.00
496
PRIMER_RIGHT_17_SELF_ANY=7.00
497
PRIMER_LEFT_17_SELF_END=3.00
498
PRIMER_RIGHT_17_SELF_END=0.00
499
PRIMER_LEFT_17_END_STABILITY=7.9000
500
PRIMER_RIGHT_17_END_STABILITY=8.2000
501
PRIMER_PAIR_17_COMPL_ANY=3.00
502
PRIMER_PAIR_17_COMPL_END=2.00
503
PRIMER_PRODUCT_SIZE_17=224
504
PRIMER_PAIR_PENALTY_18=0.9758
505
PRIMER_LEFT_18_PENALTY=0.631211
506
PRIMER_RIGHT_18_PENALTY=0.344549
507
PRIMER_LEFT_18_SEQUENCE=TTGAAGGCCACTCTCATTCA
508
PRIMER_RIGHT_18_SEQUENCE=TAAGGTCCTTCCCACAACCA
509
PRIMER_LEFT_18=63,20
510
PRIMER_RIGHT_18=294,20
511
PRIMER_LEFT_18_TM=59.369
512
PRIMER_RIGHT_18_TM=60.345
513
PRIMER_LEFT_18_GC_PERCENT=45.000
514
PRIMER_RIGHT_18_GC_PERCENT=50.000
515
PRIMER_LEFT_18_SELF_ANY=5.00
516
PRIMER_RIGHT_18_SELF_ANY=7.00
517
PRIMER_LEFT_18_SELF_END=5.00
518
PRIMER_RIGHT_18_SELF_END=0.00
519
PRIMER_LEFT_18_END_STABILITY=6.9000
520
PRIMER_RIGHT_18_END_STABILITY=8.2000
521
PRIMER_PAIR_18_COMPL_ANY=5.00
522
PRIMER_PAIR_18_COMPL_END=0.00
523
PRIMER_PRODUCT_SIZE_18=232
524
PRIMER_PAIR_PENALTY_19=1.0308
525
PRIMER_LEFT_19_PENALTY=0.686256
526
PRIMER_RIGHT_19_PENALTY=0.344549
527
PRIMER_LEFT_19_SEQUENCE=TGCAGCTGAGACTTTGAAGG
528
PRIMER_RIGHT_19_SEQUENCE=TAAGGTCCTTCCCACAACCA
529
PRIMER_LEFT_19=50,20
530
PRIMER_RIGHT_19=294,20
531
PRIMER_LEFT_19_TM=59.314
532
PRIMER_RIGHT_19_TM=60.345
533
PRIMER_LEFT_19_GC_PERCENT=50.000
534
PRIMER_RIGHT_19_GC_PERCENT=50.000
535
PRIMER_LEFT_19_SELF_ANY=6.00
536
PRIMER_RIGHT_19_SELF_ANY=7.00
537
PRIMER_LEFT_19_SELF_END=3.00
538
PRIMER_RIGHT_19_SELF_END=0.00
539
PRIMER_LEFT_19_END_STABILITY=8.2000
540
PRIMER_RIGHT_19_END_STABILITY=8.2000
541
PRIMER_PAIR_19_COMPL_ANY=5.00
542
PRIMER_PAIR_19_COMPL_END=2.00
543
PRIMER_PRODUCT_SIZE_19=245
544
PRIMER_PAIR_PENALTY_20=1.0833
545
PRIMER_LEFT_20_PENALTY=0.738752
546
PRIMER_RIGHT_20_PENALTY=0.344549
547
PRIMER_LEFT_20_SEQUENCE=TCTCATTCAGCCAGGTGTGT
548
PRIMER_RIGHT_20_SEQUENCE=TAAGGTCCTTCCCACAACCA
549
PRIMER_LEFT_20=74,20
550
PRIMER_RIGHT_20=294,20
551
PRIMER_LEFT_20_TM=59.261
552
PRIMER_RIGHT_20_TM=60.345
553
PRIMER_LEFT_20_GC_PERCENT=50.000
554
PRIMER_RIGHT_20_GC_PERCENT=50.000
555
PRIMER_LEFT_20_SELF_ANY=3.00
556
PRIMER_RIGHT_20_SELF_ANY=7.00
557
PRIMER_LEFT_20_SELF_END=1.00
558
PRIMER_RIGHT_20_SELF_END=0.00
559
PRIMER_LEFT_20_END_STABILITY=6.4000
560
PRIMER_RIGHT_20_END_STABILITY=8.2000
561
PRIMER_PAIR_20_COMPL_ANY=4.00
562
PRIMER_PAIR_20_COMPL_END=3.00
563
PRIMER_PRODUCT_SIZE_20=221
564
PRIMER_PAIR_PENALTY_21=1.1833
565
PRIMER_LEFT_21_PENALTY=0.003322
566
PRIMER_RIGHT_21_PENALTY=1.180017
567
PRIMER_LEFT_21_SEQUENCE=GCTGAGACTTTGAAGGCCAC
568
PRIMER_RIGHT_21_SEQUENCE=GTCCTTCCCACAACCAACCT
569
PRIMER_LEFT_21=54,20
570
PRIMER_RIGHT_21=290,20
571
PRIMER_LEFT_21_TM=59.997
572
PRIMER_RIGHT_21_TM=61.180
573
PRIMER_LEFT_21_GC_PERCENT=55.000
574
PRIMER_RIGHT_21_GC_PERCENT=55.000
575
PRIMER_LEFT_21_SELF_ANY=4.00
576
PRIMER_RIGHT_21_SELF_ANY=2.00
577
PRIMER_LEFT_21_SELF_END=3.00
578
PRIMER_RIGHT_21_SELF_END=0.00
579
PRIMER_LEFT_21_END_STABILITY=9.4000
580
PRIMER_RIGHT_21_END_STABILITY=7.9000
581
PRIMER_PAIR_21_COMPL_ANY=5.00
582
PRIMER_PAIR_21_COMPL_END=0.00
583
PRIMER_PRODUCT_SIZE_21=237
584
PRIMER_PAIR_PENALTY_22=1.1833
585
PRIMER_LEFT_22_PENALTY=0.003322
586
PRIMER_RIGHT_22_PENALTY=1.180017
587
PRIMER_LEFT_22_SEQUENCE=GCTGAGACTTTGAAGGCCAC
588
PRIMER_RIGHT_22_SEQUENCE=AGGTCCTTCCCACAACCAAC
589
PRIMER_LEFT_22=54,20
590
PRIMER_RIGHT_22=292,20
591
PRIMER_LEFT_22_TM=59.997
592
PRIMER_RIGHT_22_TM=61.180
593
PRIMER_LEFT_22_GC_PERCENT=55.000
594
PRIMER_RIGHT_22_GC_PERCENT=55.000
595
PRIMER_LEFT_22_SELF_ANY=4.00
596
PRIMER_RIGHT_22_SELF_ANY=5.00
597
PRIMER_LEFT_22_SELF_END=3.00
598
PRIMER_RIGHT_22_SELF_END=0.00
599
PRIMER_LEFT_22_END_STABILITY=9.4000
600
PRIMER_RIGHT_22_END_STABILITY=8.2000
601
PRIMER_PAIR_22_COMPL_ANY=5.00
602
PRIMER_PAIR_22_COMPL_END=1.00
603
PRIMER_PRODUCT_SIZE_22=239
604
PRIMER_PAIR_PENALTY_23=1.2102
605
PRIMER_LEFT_23_PENALTY=0.003322
606
PRIMER_RIGHT_23_PENALTY=1.206859
607
PRIMER_LEFT_23_SEQUENCE=GCTGAGACTTTGAAGGCCAC
608
PRIMER_RIGHT_23_SEQUENCE=GTCCTTCCCACAACCAACC
609
PRIMER_LEFT_23=54,20
610
PRIMER_RIGHT_23=290,19
611
PRIMER_LEFT_23_TM=59.997
612
PRIMER_RIGHT_23_TM=60.207
613
PRIMER_LEFT_23_GC_PERCENT=55.000
614
PRIMER_RIGHT_23_GC_PERCENT=57.895
615
PRIMER_LEFT_23_SELF_ANY=4.00
616
PRIMER_RIGHT_23_SELF_ANY=2.00
617
PRIMER_LEFT_23_SELF_END=3.00
618
PRIMER_RIGHT_23_SELF_END=0.00
619
PRIMER_LEFT_23_END_STABILITY=9.4000
620
PRIMER_RIGHT_23_END_STABILITY=8.2000
621
PRIMER_PAIR_23_COMPL_ANY=5.00
622
PRIMER_PAIR_23_COMPL_END=0.00
623
PRIMER_PRODUCT_SIZE_23=237
624
PRIMER_PAIR_PENALTY_24=1.2102
625
PRIMER_LEFT_24_PENALTY=0.003322
626
PRIMER_RIGHT_24_PENALTY=1.206859
627
PRIMER_LEFT_24_SEQUENCE=GCTGAGACTTTGAAGGCCAC
628
PRIMER_RIGHT_24_SEQUENCE=GGTCCTTCCCACAACCAAC
629
PRIMER_LEFT_24=54,20
630
PRIMER_RIGHT_24=291,19
631
PRIMER_LEFT_24_TM=59.997
632
PRIMER_RIGHT_24_TM=60.207
633
PRIMER_LEFT_24_GC_PERCENT=55.000
634
PRIMER_RIGHT_24_GC_PERCENT=57.895
635
PRIMER_LEFT_24_SELF_ANY=4.00
636
PRIMER_RIGHT_24_SELF_ANY=3.00
637
PRIMER_LEFT_24_SELF_END=3.00
638
PRIMER_RIGHT_24_SELF_END=0.00
639
PRIMER_LEFT_24_END_STABILITY=9.4000
640
PRIMER_RIGHT_24_END_STABILITY=8.2000
641
PRIMER_PAIR_24_COMPL_ANY=5.00
642
PRIMER_PAIR_24_COMPL_END=2.00
643
PRIMER_PRODUCT_SIZE_24=238
644
PRIMER_PAIR_PENALTY_25=1.2227
645
PRIMER_LEFT_25_PENALTY=0.042732
646
PRIMER_RIGHT_25_PENALTY=1.180017
647
PRIMER_LEFT_25_SEQUENCE=GAAGGCCACTCTCATTCAGC
648
PRIMER_RIGHT_25_SEQUENCE=GTCCTTCCCACAACCAACCT
649
PRIMER_LEFT_25=65,20
650
PRIMER_RIGHT_25=290,20
651
PRIMER_LEFT_25_TM=59.957
652
PRIMER_RIGHT_25_TM=61.180
653
PRIMER_LEFT_25_GC_PERCENT=55.000
654
PRIMER_RIGHT_25_GC_PERCENT=55.000
655
PRIMER_LEFT_25_SELF_ANY=4.00
656
PRIMER_RIGHT_25_SELF_ANY=2.00
657
PRIMER_LEFT_25_SELF_END=2.00
658
PRIMER_RIGHT_25_SELF_END=0.00
659
PRIMER_LEFT_25_END_STABILITY=8.2000
660
PRIMER_RIGHT_25_END_STABILITY=7.9000
661
PRIMER_PAIR_25_COMPL_ANY=5.00
662
PRIMER_PAIR_25_COMPL_END=1.00
663
PRIMER_PRODUCT_SIZE_25=226
664
PRIMER_PAIR_PENALTY_26=1.2227
665
PRIMER_LEFT_26_PENALTY=0.042732
666
PRIMER_RIGHT_26_PENALTY=1.180017
667
PRIMER_LEFT_26_SEQUENCE=GAAGGCCACTCTCATTCAGC
668
PRIMER_RIGHT_26_SEQUENCE=AGGTCCTTCCCACAACCAAC
669
PRIMER_LEFT_26=65,20
670
PRIMER_RIGHT_26=292,20
671
PRIMER_LEFT_26_TM=59.957
672
PRIMER_RIGHT_26_TM=61.180
673
PRIMER_LEFT_26_GC_PERCENT=55.000
674
PRIMER_RIGHT_26_GC_PERCENT=55.000
675
PRIMER_LEFT_26_SELF_ANY=4.00
676
PRIMER_RIGHT_26_SELF_ANY=5.00
677
PRIMER_LEFT_26_SELF_END=2.00
678
PRIMER_RIGHT_26_SELF_END=0.00
679
PRIMER_LEFT_26_END_STABILITY=8.2000
680
PRIMER_RIGHT_26_END_STABILITY=8.2000
681
PRIMER_PAIR_26_COMPL_ANY=5.00
682
PRIMER_PAIR_26_COMPL_END=0.00
683
PRIMER_PRODUCT_SIZE_26=228
684
PRIMER_PAIR_PENALTY_27=1.2496
685
PRIMER_LEFT_27_PENALTY=0.042732
686
PRIMER_RIGHT_27_PENALTY=1.206859
687
PRIMER_LEFT_27_SEQUENCE=GAAGGCCACTCTCATTCAGC
688
PRIMER_RIGHT_27_SEQUENCE=GTCCTTCCCACAACCAACC
689
PRIMER_LEFT_27=65,20
690
PRIMER_RIGHT_27=290,19
691
PRIMER_LEFT_27_TM=59.957
692
PRIMER_RIGHT_27_TM=60.207
693
PRIMER_LEFT_27_GC_PERCENT=55.000
694
PRIMER_RIGHT_27_GC_PERCENT=57.895
695
PRIMER_LEFT_27_SELF_ANY=4.00
696
PRIMER_RIGHT_27_SELF_ANY=2.00
697
PRIMER_LEFT_27_SELF_END=2.00
698
PRIMER_RIGHT_27_SELF_END=0.00
699
PRIMER_LEFT_27_END_STABILITY=8.2000
700
PRIMER_RIGHT_27_END_STABILITY=8.2000
701
PRIMER_PAIR_27_COMPL_ANY=5.00
702
PRIMER_PAIR_27_COMPL_END=0.00
703
PRIMER_PRODUCT_SIZE_27=226
704
PRIMER_PAIR_PENALTY_28=1.2496
705
PRIMER_LEFT_28_PENALTY=0.042732
706
PRIMER_RIGHT_28_PENALTY=1.206859
707
PRIMER_LEFT_28_SEQUENCE=GAAGGCCACTCTCATTCAGC
708
PRIMER_RIGHT_28_SEQUENCE=GGTCCTTCCCACAACCAAC
709
PRIMER_LEFT_28=65,20
710
PRIMER_RIGHT_28=291,19
711
PRIMER_LEFT_28_TM=59.957
712
PRIMER_RIGHT_28_TM=60.207
713
PRIMER_LEFT_28_GC_PERCENT=55.000
714
PRIMER_RIGHT_28_GC_PERCENT=57.895
715
PRIMER_LEFT_28_SELF_ANY=4.00
716
PRIMER_RIGHT_28_SELF_ANY=3.00
717
PRIMER_LEFT_28_SELF_END=2.00
718
PRIMER_RIGHT_28_SELF_END=0.00
719
PRIMER_LEFT_28_END_STABILITY=8.2000
720
PRIMER_RIGHT_28_END_STABILITY=8.2000
721
PRIMER_PAIR_28_COMPL_ANY=5.00
722
PRIMER_PAIR_28_COMPL_END=0.00
723
PRIMER_PRODUCT_SIZE_28=227
724
PRIMER_PAIR_PENALTY_29=1.3138
725
PRIMER_LEFT_29_PENALTY=0.133741
726
PRIMER_RIGHT_29_PENALTY=1.180017
727
PRIMER_LEFT_29_SEQUENCE=AGCTGAGACTTTGAAGGCCA
728
PRIMER_RIGHT_29_SEQUENCE=GTCCTTCCCACAACCAACCT
729
PRIMER_LEFT_29=53,20
730
PRIMER_RIGHT_29=290,20
731
PRIMER_LEFT_29_TM=60.134
732
PRIMER_RIGHT_29_TM=61.180
733
PRIMER_LEFT_29_GC_PERCENT=50.000
734
PRIMER_RIGHT_29_GC_PERCENT=55.000
735
PRIMER_LEFT_29_SELF_ANY=4.00
736
PRIMER_RIGHT_29_SELF_ANY=2.00
737
PRIMER_LEFT_29_SELF_END=4.00
738
PRIMER_RIGHT_29_SELF_END=0.00
739
PRIMER_LEFT_29_END_STABILITY=11.2000
740
PRIMER_RIGHT_29_END_STABILITY=7.9000
741
PRIMER_PAIR_29_COMPL_ANY=5.00
742
PRIMER_PAIR_29_COMPL_END=1.00
743
PRIMER_PRODUCT_SIZE_29=238
744
PRIMER_PAIR_PENALTY_30=1.3138
745
PRIMER_LEFT_30_PENALTY=0.133741
746
PRIMER_RIGHT_30_PENALTY=1.180017
747
PRIMER_LEFT_30_SEQUENCE=CAGCTGAGACTTTGAAGGCC
748
PRIMER_RIGHT_30_SEQUENCE=GTCCTTCCCACAACCAACCT
749
PRIMER_LEFT_30=52,20
750
PRIMER_RIGHT_30=290,20
751
PRIMER_LEFT_30_TM=60.134
752
PRIMER_RIGHT_30_TM=61.180
753
PRIMER_LEFT_30_GC_PERCENT=55.000
754
PRIMER_RIGHT_30_GC_PERCENT=55.000
755
PRIMER_LEFT_30_SELF_ANY=6.00
756
PRIMER_RIGHT_30_SELF_ANY=2.00
757
PRIMER_LEFT_30_SELF_END=4.00
758
PRIMER_RIGHT_30_SELF_END=0.00
759
PRIMER_LEFT_30_END_STABILITY=10.9000
760
PRIMER_RIGHT_30_END_STABILITY=7.9000
761
PRIMER_PAIR_30_COMPL_ANY=5.00
762
PRIMER_PAIR_30_COMPL_END=1.00
763
PRIMER_PRODUCT_SIZE_30=239
764
PRIMER_PAIR_PENALTY_31=1.3138
765
PRIMER_LEFT_31_PENALTY=0.133741
766
PRIMER_RIGHT_31_PENALTY=1.180017
767
PRIMER_LEFT_31_SEQUENCE=AGCTGAGACTTTGAAGGCCA
768
PRIMER_RIGHT_31_SEQUENCE=AGGTCCTTCCCACAACCAAC
769
PRIMER_LEFT_31=53,20
770
PRIMER_RIGHT_31=292,20
771
PRIMER_LEFT_31_TM=60.134
772
PRIMER_RIGHT_31_TM=61.180
773
PRIMER_LEFT_31_GC_PERCENT=50.000
774
PRIMER_RIGHT_31_GC_PERCENT=55.000
775
PRIMER_LEFT_31_SELF_ANY=4.00
776
PRIMER_RIGHT_31_SELF_ANY=5.00
777
PRIMER_LEFT_31_SELF_END=4.00
778
PRIMER_RIGHT_31_SELF_END=0.00
779
PRIMER_LEFT_31_END_STABILITY=11.2000
780
PRIMER_RIGHT_31_END_STABILITY=8.2000
781
PRIMER_PAIR_31_COMPL_ANY=5.00
782
PRIMER_PAIR_31_COMPL_END=1.00
783
PRIMER_PRODUCT_SIZE_31=240
784
PRIMER_PAIR_PENALTY_32=1.3138
785
PRIMER_LEFT_32_PENALTY=0.133741
786
PRIMER_RIGHT_32_PENALTY=1.180017
787
PRIMER_LEFT_32_SEQUENCE=CAGCTGAGACTTTGAAGGCC
788
PRIMER_RIGHT_32_SEQUENCE=AGGTCCTTCCCACAACCAAC
789
PRIMER_LEFT_32=52,20
790
PRIMER_RIGHT_32=292,20
791
PRIMER_LEFT_32_TM=60.134
792
PRIMER_RIGHT_32_TM=61.180
793
PRIMER_LEFT_32_GC_PERCENT=55.000
794
PRIMER_RIGHT_32_GC_PERCENT=55.000
795
PRIMER_LEFT_32_SELF_ANY=6.00
796
PRIMER_RIGHT_32_SELF_ANY=5.00
797
PRIMER_LEFT_32_SELF_END=4.00
798
PRIMER_RIGHT_32_SELF_END=0.00
799
PRIMER_LEFT_32_END_STABILITY=10.9000
800
PRIMER_RIGHT_32_END_STABILITY=8.2000
801
PRIMER_PAIR_32_COMPL_ANY=5.00
802
PRIMER_PAIR_32_COMPL_END=2.00
803
PRIMER_PRODUCT_SIZE_32=241
804
PRIMER_PAIR_PENALTY_33=1.3406
805
PRIMER_LEFT_33_PENALTY=0.133741
806
PRIMER_RIGHT_33_PENALTY=1.206859
807
PRIMER_LEFT_33_SEQUENCE=AGCTGAGACTTTGAAGGCCA
808
PRIMER_RIGHT_33_SEQUENCE=GTCCTTCCCACAACCAACC
809
PRIMER_LEFT_33=53,20
810
PRIMER_RIGHT_33=290,19
811
PRIMER_LEFT_33_TM=60.134
812
PRIMER_RIGHT_33_TM=60.207
813
PRIMER_LEFT_33_GC_PERCENT=50.000
814
PRIMER_RIGHT_33_GC_PERCENT=57.895
815
PRIMER_LEFT_33_SELF_ANY=4.00
816
PRIMER_RIGHT_33_SELF_ANY=2.00
817
PRIMER_LEFT_33_SELF_END=4.00
818
PRIMER_RIGHT_33_SELF_END=0.00
819
PRIMER_LEFT_33_END_STABILITY=11.2000
820
PRIMER_RIGHT_33_END_STABILITY=8.2000
821
PRIMER_PAIR_33_COMPL_ANY=5.00
822
PRIMER_PAIR_33_COMPL_END=1.00
823
PRIMER_PRODUCT_SIZE_33=238
824
PRIMER_PAIR_PENALTY_34=1.3406
825
PRIMER_LEFT_34_PENALTY=0.133741
826
PRIMER_RIGHT_34_PENALTY=1.206859
827
PRIMER_LEFT_34_SEQUENCE=CAGCTGAGACTTTGAAGGCC
828
PRIMER_RIGHT_34_SEQUENCE=GTCCTTCCCACAACCAACC
829
PRIMER_LEFT_34=52,20
830
PRIMER_RIGHT_34=290,19
831
PRIMER_LEFT_34_TM=60.134
832
PRIMER_RIGHT_34_TM=60.207
833
PRIMER_LEFT_34_GC_PERCENT=55.000
834
PRIMER_RIGHT_34_GC_PERCENT=57.895
835
PRIMER_LEFT_34_SELF_ANY=6.00
836
PRIMER_RIGHT_34_SELF_ANY=2.00
837
PRIMER_LEFT_34_SELF_END=4.00
838
PRIMER_RIGHT_34_SELF_END=0.00
839
PRIMER_LEFT_34_END_STABILITY=10.9000
840
PRIMER_RIGHT_34_END_STABILITY=8.2000
841
PRIMER_PAIR_34_COMPL_ANY=5.00
842
PRIMER_PAIR_34_COMPL_END=1.00
843
PRIMER_PRODUCT_SIZE_34=239
844
PRIMER_PAIR_PENALTY_35=1.3406
845
PRIMER_LEFT_35_PENALTY=0.133741
846
PRIMER_RIGHT_35_PENALTY=1.206859
847
PRIMER_LEFT_35_SEQUENCE=AGCTGAGACTTTGAAGGCCA
848
PRIMER_RIGHT_35_SEQUENCE=GGTCCTTCCCACAACCAAC
849
PRIMER_LEFT_35=53,20
850
PRIMER_RIGHT_35=291,19
851
PRIMER_LEFT_35_TM=60.134
852
PRIMER_RIGHT_35_TM=60.207
853
PRIMER_LEFT_35_GC_PERCENT=50.000
854
PRIMER_RIGHT_35_GC_PERCENT=57.895
855
PRIMER_LEFT_35_SELF_ANY=4.00
856
PRIMER_RIGHT_35_SELF_ANY=3.00
857
PRIMER_LEFT_35_SELF_END=4.00
858
PRIMER_RIGHT_35_SELF_END=0.00
859
PRIMER_LEFT_35_END_STABILITY=11.2000
860
PRIMER_RIGHT_35_END_STABILITY=8.2000
861
PRIMER_PAIR_35_COMPL_ANY=5.00
862
PRIMER_PAIR_35_COMPL_END=2.00
863
PRIMER_PRODUCT_SIZE_35=239
864
PRIMER_PAIR_PENALTY_36=1.3406
865
PRIMER_LEFT_36_PENALTY=0.133741
866
PRIMER_RIGHT_36_PENALTY=1.206859
867
PRIMER_LEFT_36_SEQUENCE=CAGCTGAGACTTTGAAGGCC
868
PRIMER_RIGHT_36_SEQUENCE=GGTCCTTCCCACAACCAAC
869
PRIMER_LEFT_36=52,20
870
PRIMER_RIGHT_36=291,19
871
PRIMER_LEFT_36_TM=60.134
872
PRIMER_RIGHT_36_TM=60.207
873
PRIMER_LEFT_36_GC_PERCENT=55.000
874
PRIMER_RIGHT_36_GC_PERCENT=57.895
875
PRIMER_LEFT_36_SELF_ANY=6.00
876
PRIMER_RIGHT_36_SELF_ANY=3.00
877
PRIMER_LEFT_36_SELF_END=4.00
878
PRIMER_RIGHT_36_SELF_END=0.00
879
PRIMER_LEFT_36_END_STABILITY=10.9000
880
PRIMER_RIGHT_36_END_STABILITY=8.2000
881
PRIMER_PAIR_36_COMPL_ANY=5.00
882
PRIMER_PAIR_36_COMPL_END=2.00
883
PRIMER_PRODUCT_SIZE_36=240
884
PRIMER_PAIR_PENALTY_37=1.3504
885
PRIMER_LEFT_37_PENALTY=0.003322
886
PRIMER_RIGHT_37_PENALTY=1.347100
887
PRIMER_LEFT_37_SEQUENCE=GCTGAGACTTTGAAGGCCAC
888
PRIMER_RIGHT_37_SEQUENCE=TCCTTCCCACAACCAACCT
889
PRIMER_LEFT_37=54,20
890
PRIMER_RIGHT_37=289,19
891
PRIMER_LEFT_37_TM=59.997
892
PRIMER_RIGHT_37_TM=60.347
893
PRIMER_LEFT_37_GC_PERCENT=55.000
894
PRIMER_RIGHT_37_GC_PERCENT=52.632
895
PRIMER_LEFT_37_SELF_ANY=4.00
896
PRIMER_RIGHT_37_SELF_ANY=2.00
897
PRIMER_LEFT_37_SELF_END=3.00
898
PRIMER_RIGHT_37_SELF_END=0.00
899
PRIMER_LEFT_37_END_STABILITY=9.4000
900
PRIMER_RIGHT_37_END_STABILITY=7.9000
901
PRIMER_PAIR_37_COMPL_ANY=5.00
902
PRIMER_PAIR_37_COMPL_END=0.00
903
PRIMER_PRODUCT_SIZE_37=236
904
PRIMER_PAIR_PENALTY_38=1.3504
905
PRIMER_LEFT_38_PENALTY=0.003322
906
PRIMER_RIGHT_38_PENALTY=1.347100
907
PRIMER_LEFT_38_SEQUENCE=GCTGAGACTTTGAAGGCCAC
908
PRIMER_RIGHT_38_SEQUENCE=AGGTCCTTCCCACAACCAA
909
PRIMER_LEFT_38=54,20
910
PRIMER_RIGHT_38=292,19
911
PRIMER_LEFT_38_TM=59.997
912
PRIMER_RIGHT_38_TM=60.347
913
PRIMER_LEFT_38_GC_PERCENT=55.000
914
PRIMER_RIGHT_38_GC_PERCENT=52.632
915
PRIMER_LEFT_38_SELF_ANY=4.00
916
PRIMER_RIGHT_38_SELF_ANY=5.00
917
PRIMER_LEFT_38_SELF_END=3.00
918
PRIMER_RIGHT_38_SELF_END=0.00
919
PRIMER_LEFT_38_END_STABILITY=9.4000
920
PRIMER_RIGHT_38_END_STABILITY=8.2000
921
PRIMER_PAIR_38_COMPL_ANY=5.00
922
PRIMER_PAIR_38_COMPL_END=0.00
923
PRIMER_PRODUCT_SIZE_38=239
924
PRIMER_PAIR_PENALTY_39=1.3504
925
PRIMER_LEFT_39_PENALTY=0.003322
926
PRIMER_RIGHT_39_PENALTY=1.347100
927
PRIMER_LEFT_39_SEQUENCE=GCTGAGACTTTGAAGGCCAC
928
PRIMER_RIGHT_39_SEQUENCE=AAGGTCCTTCCCACAACCA
929
PRIMER_LEFT_39=54,20
930
PRIMER_RIGHT_39=293,19
931
PRIMER_LEFT_39_TM=59.997
932
PRIMER_RIGHT_39_TM=60.347
933
PRIMER_LEFT_39_GC_PERCENT=55.000
934
PRIMER_RIGHT_39_GC_PERCENT=52.632
935
PRIMER_LEFT_39_SELF_ANY=4.00
936
PRIMER_RIGHT_39_SELF_ANY=7.00
937
PRIMER_LEFT_39_SELF_END=3.00
938
PRIMER_RIGHT_39_SELF_END=0.00
939
PRIMER_LEFT_39_END_STABILITY=9.4000
940
PRIMER_RIGHT_39_END_STABILITY=8.2000
941
PRIMER_PAIR_39_COMPL_ANY=5.00
942
PRIMER_PAIR_39_COMPL_END=1.00
943
PRIMER_PRODUCT_SIZE_39=240
944
=
945
MARKER_NAME=06.RHAP34FE6.seq
946
SEQUENCE=NNNNNNNNCCAACCCAAAAATACAGACGGANACACACACAAACAGAGACACAGACACAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGAGACGGGTAACACAGGGNATCTTGACTGATATAGAGGTACATGTCCAAACAAGAATAGGGTAGCGGGAAAGCACAGAAGAGAAGAAAAGACAGAGTTTTGCAGACCTTGGATTTTACCCTTATGCCACCTCACCAAAGATGTGATTCCCTATATGGTCAAAGA
947
INCLUDED_REGION=8,287
948
TARGET=61,68,CA
949
PRIMER_PAIR_PENALTY=2.0965
950
PRIMER_LEFT_PENALTY=1.153194
951
PRIMER_RIGHT_PENALTY=0.943292
952
PRIMER_LEFT_SEQUENCE=CAACCCAAAAATACAGACGGA
953
PRIMER_RIGHT_SEQUENCE=CCCTGTGTTACCCGTCTCAA
954
PRIMER_LEFT=9,21
955
PRIMER_RIGHT=148,20
956
PRIMER_LEFT_TM=59.847
957
PRIMER_RIGHT_TM=60.943
958
PRIMER_LEFT_GC_PERCENT=42.857
959
PRIMER_RIGHT_GC_PERCENT=55.000
960
PRIMER_LEFT_SELF_ANY=2.00
961
PRIMER_RIGHT_SELF_ANY=3.00
962
PRIMER_LEFT_SELF_END=0.00
963
PRIMER_RIGHT_SELF_END=0.00
964
PRIMER_LEFT_END_STABILITY=9.6000
965
PRIMER_RIGHT_END_STABILITY=7.0000
966
PRIMER_PAIR_COMPL_ANY=6.00
967
PRIMER_PAIR_COMPL_END=4.00
968
PRIMER_PRODUCT_SIZE=140
969
PRIMER_PAIR_PENALTY_1=2.6236
970
PRIMER_LEFT_1_PENALTY=1.153194
971
PRIMER_RIGHT_1_PENALTY=1.470379
972
PRIMER_LEFT_1_SEQUENCE=CAACCCAAAAATACAGACGGA
973
PRIMER_RIGHT_1_SEQUENCE=CCCTGTGTTACCCGTCTCA
974
PRIMER_LEFT_1=9,21
975
PRIMER_RIGHT_1=148,19
976
PRIMER_LEFT_1_TM=59.847
977
PRIMER_RIGHT_1_TM=59.530
978
PRIMER_LEFT_1_GC_PERCENT=42.857
979
PRIMER_RIGHT_1_GC_PERCENT=57.895
980
PRIMER_LEFT_1_SELF_ANY=2.00
981
PRIMER_RIGHT_1_SELF_ANY=3.00
982
PRIMER_LEFT_1_SELF_END=0.00
983
PRIMER_RIGHT_1_SELF_END=2.00
984
PRIMER_LEFT_1_END_STABILITY=9.6000
985
PRIMER_RIGHT_1_END_STABILITY=6.7000
986
PRIMER_PAIR_1_COMPL_ANY=6.00
987
PRIMER_PAIR_1_COMPL_END=3.00
988
PRIMER_PRODUCT_SIZE_1=140
989
PRIMER_PAIR_PENALTY_2=2.9431
990
PRIMER_LEFT_2_PENALTY=1.999797
991
PRIMER_RIGHT_2_PENALTY=0.943292
992
PRIMER_LEFT_2_SEQUENCE=CCAACCCAAAAATACAGACG
993
PRIMER_RIGHT_2_SEQUENCE=CCCTGTGTTACCCGTCTCAA
994
PRIMER_LEFT_2=8,20
995
PRIMER_RIGHT_2=148,20
996
PRIMER_LEFT_2_TM=58.000
997
PRIMER_RIGHT_2_TM=60.943
998
PRIMER_LEFT_2_GC_PERCENT=45.000
999
PRIMER_RIGHT_2_GC_PERCENT=55.000
1000
PRIMER_LEFT_2_SELF_ANY=2.00
1001
PRIMER_RIGHT_2_SELF_ANY=3.00
1002
PRIMER_LEFT_2_SELF_END=2.00
1003
PRIMER_RIGHT_2_SELF_END=0.00
1004
PRIMER_LEFT_2_END_STABILITY=8.1000
1005
PRIMER_RIGHT_2_END_STABILITY=7.0000
1006
PRIMER_PAIR_2_COMPL_ANY=5.00
1007
PRIMER_PAIR_2_COMPL_END=4.00
1008
PRIMER_PRODUCT_SIZE_2=141
1009
PRIMER_PAIR_PENALTY_3=2.9431
1010
PRIMER_LEFT_3_PENALTY=1.999797
1011
PRIMER_RIGHT_3_PENALTY=0.943292
1012
PRIMER_LEFT_3_SEQUENCE=CAACCCAAAAATACAGACGG
1013
PRIMER_RIGHT_3_SEQUENCE=CCCTGTGTTACCCGTCTCAA
1014
PRIMER_LEFT_3=9,20
1015
PRIMER_RIGHT_3=148,20
1016
PRIMER_LEFT_3_TM=58.000
1017
PRIMER_RIGHT_3_TM=60.943
1018
PRIMER_LEFT_3_GC_PERCENT=45.000
1019
PRIMER_RIGHT_3_GC_PERCENT=55.000
1020
PRIMER_LEFT_3_SELF_ANY=2.00
1021
PRIMER_RIGHT_3_SELF_ANY=3.00
1022
PRIMER_LEFT_3_SELF_END=1.00
1023
PRIMER_RIGHT_3_SELF_END=0.00
1024
PRIMER_LEFT_3_END_STABILITY=9.6000
1025
PRIMER_RIGHT_3_END_STABILITY=7.0000
1026
PRIMER_PAIR_3_COMPL_ANY=6.00
1027
PRIMER_PAIR_3_COMPL_END=5.00
1028
PRIMER_PRODUCT_SIZE_3=140
1029
PRIMER_PAIR_PENALTY_4=3.4069
1030
PRIMER_LEFT_4_PENALTY=2.463612
1031
PRIMER_RIGHT_4_PENALTY=0.943292
1032
PRIMER_LEFT_4_SEQUENCE=CCAACCCAAAAATACAGACGG
1033
PRIMER_RIGHT_4_SEQUENCE=CCCTGTGTTACCCGTCTCAA
1034
PRIMER_LEFT_4=8,21
1035
PRIMER_RIGHT_4=148,20
1036
PRIMER_LEFT_4_TM=61.464
1037
PRIMER_RIGHT_4_TM=60.943
1038
PRIMER_LEFT_4_GC_PERCENT=47.619
1039
PRIMER_RIGHT_4_GC_PERCENT=55.000
1040
PRIMER_LEFT_4_SELF_ANY=2.00
1041
PRIMER_RIGHT_4_SELF_ANY=3.00
1042
PRIMER_LEFT_4_SELF_END=1.00
1043
PRIMER_RIGHT_4_SELF_END=0.00
1044
PRIMER_LEFT_4_END_STABILITY=9.6000
1045
PRIMER_RIGHT_4_END_STABILITY=7.0000
1046
PRIMER_PAIR_4_COMPL_ANY=6.00
1047
PRIMER_PAIR_4_COMPL_END=5.00
1048
PRIMER_PRODUCT_SIZE_4=141
1049
PRIMER_PAIR_PENALTY_5=3.4702
1050
PRIMER_LEFT_5_PENALTY=1.999797
1051
PRIMER_RIGHT_5_PENALTY=1.470379
1052
PRIMER_LEFT_5_SEQUENCE=CCAACCCAAAAATACAGACG
1053
PRIMER_RIGHT_5_SEQUENCE=CCCTGTGTTACCCGTCTCA
1054
PRIMER_LEFT_5=8,20
1055
PRIMER_RIGHT_5=148,19
1056
PRIMER_LEFT_5_TM=58.000
1057
PRIMER_RIGHT_5_TM=59.530
1058
PRIMER_LEFT_5_GC_PERCENT=45.000
1059
PRIMER_RIGHT_5_GC_PERCENT=57.895
1060
PRIMER_LEFT_5_SELF_ANY=2.00
1061
PRIMER_RIGHT_5_SELF_ANY=3.00
1062
PRIMER_LEFT_5_SELF_END=2.00
1063
PRIMER_RIGHT_5_SELF_END=2.00
1064
PRIMER_LEFT_5_END_STABILITY=8.1000
1065
PRIMER_RIGHT_5_END_STABILITY=6.7000
1066
PRIMER_PAIR_5_COMPL_ANY=5.00
1067
PRIMER_PAIR_5_COMPL_END=3.00
1068
PRIMER_PRODUCT_SIZE_5=141
1069
PRIMER_PAIR_PENALTY_6=3.4702
1070
PRIMER_LEFT_6_PENALTY=1.999797
1071
PRIMER_RIGHT_6_PENALTY=1.470379
1072
PRIMER_LEFT_6_SEQUENCE=CAACCCAAAAATACAGACGG
1073
PRIMER_RIGHT_6_SEQUENCE=CCCTGTGTTACCCGTCTCA
1074
PRIMER_LEFT_6=9,20
1075
PRIMER_RIGHT_6=148,19
1076
PRIMER_LEFT_6_TM=58.000
1077
PRIMER_RIGHT_6_TM=59.530
1078
PRIMER_LEFT_6_GC_PERCENT=45.000
1079
PRIMER_RIGHT_6_GC_PERCENT=57.895
1080
PRIMER_LEFT_6_SELF_ANY=2.00
1081
PRIMER_RIGHT_6_SELF_ANY=3.00
1082
PRIMER_LEFT_6_SELF_END=1.00
1083
PRIMER_RIGHT_6_SELF_END=2.00
1084
PRIMER_LEFT_6_END_STABILITY=9.6000
1085
PRIMER_RIGHT_6_END_STABILITY=6.7000
1086
PRIMER_PAIR_6_COMPL_ANY=6.00
1087
PRIMER_PAIR_6_COMPL_END=4.00
1088
PRIMER_PRODUCT_SIZE_6=140
1089
PRIMER_PAIR_PENALTY_7=3.8768
1090
PRIMER_LEFT_7_PENALTY=2.933539
1091
PRIMER_RIGHT_7_PENALTY=0.943292
1092
PRIMER_LEFT_7_SEQUENCE=AACCCAAAAATACAGACGGA
1093
PRIMER_RIGHT_7_SEQUENCE=CCCTGTGTTACCCGTCTCAA
1094
PRIMER_LEFT_7=10,20
1095
PRIMER_RIGHT_7=148,20
1096
PRIMER_LEFT_7_TM=57.066
1097
PRIMER_RIGHT_7_TM=60.943
1098
PRIMER_LEFT_7_GC_PERCENT=40.000
1099
PRIMER_RIGHT_7_GC_PERCENT=55.000
1100
PRIMER_LEFT_7_SELF_ANY=2.00
1101
PRIMER_RIGHT_7_SELF_ANY=3.00
1102
PRIMER_LEFT_7_SELF_END=0.00
1103
PRIMER_RIGHT_7_SELF_END=0.00
1104
PRIMER_LEFT_7_END_STABILITY=9.6000
1105
PRIMER_RIGHT_7_END_STABILITY=7.0000
1106
PRIMER_PAIR_7_COMPL_ANY=6.00
1107
PRIMER_PAIR_7_COMPL_END=4.00
1108
PRIMER_PRODUCT_SIZE_7=139
1109
PRIMER_PAIR_PENALTY_8=3.9340
1110
PRIMER_LEFT_8_PENALTY=2.463612
1111
PRIMER_RIGHT_8_PENALTY=1.470379
1112
PRIMER_LEFT_8_SEQUENCE=CCAACCCAAAAATACAGACGG
1113
PRIMER_RIGHT_8_SEQUENCE=CCCTGTGTTACCCGTCTCA
1114
PRIMER_LEFT_8=8,21
1115
PRIMER_RIGHT_8=148,19
1116
PRIMER_LEFT_8_TM=61.464
1117
PRIMER_RIGHT_8_TM=59.530
1118
PRIMER_LEFT_8_GC_PERCENT=47.619
1119
PRIMER_RIGHT_8_GC_PERCENT=57.895
1120
PRIMER_LEFT_8_SELF_ANY=2.00
1121
PRIMER_RIGHT_8_SELF_ANY=3.00
1122
PRIMER_LEFT_8_SELF_END=1.00
1123
PRIMER_RIGHT_8_SELF_END=2.00
1124
PRIMER_LEFT_8_END_STABILITY=9.6000
1125
PRIMER_RIGHT_8_END_STABILITY=6.7000
1126
PRIMER_PAIR_8_COMPL_ANY=6.00
1127
PRIMER_PAIR_8_COMPL_END=4.00
1128
PRIMER_PRODUCT_SIZE_8=141
1129
PRIMER_PAIR_PENALTY_9=4.4039
1130
PRIMER_LEFT_9_PENALTY=2.933539
1131
PRIMER_RIGHT_9_PENALTY=1.470379
1132
PRIMER_LEFT_9_SEQUENCE=AACCCAAAAATACAGACGGA
1133
PRIMER_RIGHT_9_SEQUENCE=CCCTGTGTTACCCGTCTCA
1134
PRIMER_LEFT_9=10,20
1135
PRIMER_RIGHT_9=148,19
1136
PRIMER_LEFT_9_TM=57.066
1137
PRIMER_RIGHT_9_TM=59.530
1138
PRIMER_LEFT_9_GC_PERCENT=40.000
1139
PRIMER_RIGHT_9_GC_PERCENT=57.895
1140
PRIMER_LEFT_9_SELF_ANY=2.00
1141
PRIMER_RIGHT_9_SELF_ANY=3.00
1142
PRIMER_LEFT_9_SELF_END=0.00
1143
PRIMER_RIGHT_9_SELF_END=2.00
1144
PRIMER_LEFT_9_END_STABILITY=9.6000
1145
PRIMER_RIGHT_9_END_STABILITY=6.7000
1146
PRIMER_PAIR_9_COMPL_ANY=6.00
1147
PRIMER_PAIR_9_COMPL_END=3.00
1148
PRIMER_PRODUCT_SIZE_9=139
1149
PRIMER_PAIR_PENALTY_10=4.4517
1150
PRIMER_LEFT_10_PENALTY=4.436430
1151
PRIMER_RIGHT_10_PENALTY=0.015253
1152
PRIMER_LEFT_10_SEQUENCE=CACAAACAGAGACACAGACACAGA
1153
PRIMER_RIGHT_10_SEQUENCE=CTTCTGTGCTTTCCCGCTAC
1154
PRIMER_LEFT_10=36,24
1155
PRIMER_RIGHT_10=210,20
1156
PRIMER_LEFT_10_TM=60.436
1157
PRIMER_RIGHT_10_TM=60.015
1158
PRIMER_LEFT_10_GC_PERCENT=45.833
1159
PRIMER_RIGHT_10_GC_PERCENT=55.000
1160
PRIMER_LEFT_10_SELF_ANY=1.00
1161
PRIMER_RIGHT_10_SELF_ANY=2.00
1162
PRIMER_LEFT_10_SELF_END=0.00
1163
PRIMER_RIGHT_10_SELF_END=0.00
1164
PRIMER_LEFT_10_END_STABILITY=6.4000
1165
PRIMER_RIGHT_10_END_STABILITY=6.9000
1166
PRIMER_PAIR_10_COMPL_ANY=6.00
1167
PRIMER_PAIR_10_COMPL_END=4.00
1168
PRIMER_PRODUCT_SIZE_10=175
1169
PRIMER_PAIR_PENALTY_11=4.4977
1170
PRIMER_LEFT_11_PENALTY=4.436430
1171
PRIMER_RIGHT_11_PENALTY=0.061230
1172
PRIMER_LEFT_11_SEQUENCE=CACAAACAGAGACACAGACACAGA
1173
PRIMER_RIGHT_11_SEQUENCE=GCTTTCCCGCTACCCTATTC
1174
PRIMER_LEFT_11=36,24
1175
PRIMER_RIGHT_11=203,20
1176
PRIMER_LEFT_11_TM=60.436
1177
PRIMER_RIGHT_11_TM=60.061
1178
PRIMER_LEFT_11_GC_PERCENT=45.833
1179
PRIMER_RIGHT_11_GC_PERCENT=55.000
1180
PRIMER_LEFT_11_SELF_ANY=1.00
1181
PRIMER_RIGHT_11_SELF_ANY=2.00
1182
PRIMER_LEFT_11_SELF_END=0.00
1183
PRIMER_RIGHT_11_SELF_END=0.00
1184
PRIMER_LEFT_11_END_STABILITY=6.4000
1185
PRIMER_RIGHT_11_END_STABILITY=5.9000
1186
PRIMER_PAIR_11_COMPL_ANY=3.00
1187
PRIMER_PAIR_11_COMPL_END=2.00
1188
PRIMER_PRODUCT_SIZE_11=168
1189
PRIMER_PAIR_PENALTY_12=4.5292
1190
PRIMER_LEFT_12_PENALTY=4.513917
1191
PRIMER_RIGHT_12_PENALTY=0.015253
1192
PRIMER_LEFT_12_SEQUENCE=CACAAACAGAGACACAGACACAG
1193
PRIMER_RIGHT_12_SEQUENCE=CTTCTGTGCTTTCCCGCTAC
1194
PRIMER_LEFT_12=36,23
1195
PRIMER_RIGHT_12=210,20
1196
PRIMER_LEFT_12_TM=58.486
1197
PRIMER_RIGHT_12_TM=60.015
1198
PRIMER_LEFT_12_GC_PERCENT=47.826
1199
PRIMER_RIGHT_12_GC_PERCENT=55.000
1200
PRIMER_LEFT_12_SELF_ANY=1.00
1201
PRIMER_RIGHT_12_SELF_ANY=2.00
1202
PRIMER_LEFT_12_SELF_END=1.00
1203
PRIMER_RIGHT_12_SELF_END=0.00
1204
PRIMER_LEFT_12_END_STABILITY=6.7000
1205
PRIMER_RIGHT_12_END_STABILITY=6.9000
1206
PRIMER_PAIR_12_COMPL_ANY=6.00
1207
PRIMER_PAIR_12_COMPL_END=3.00
1208
PRIMER_PRODUCT_SIZE_12=175
1209
PRIMER_PAIR_PENALTY_13=4.5751
1210
PRIMER_LEFT_13_PENALTY=4.513917
1211
PRIMER_RIGHT_13_PENALTY=0.061230
1212
PRIMER_LEFT_13_SEQUENCE=CACAAACAGAGACACAGACACAG
1213
PRIMER_RIGHT_13_SEQUENCE=GCTTTCCCGCTACCCTATTC
1214
PRIMER_LEFT_13=36,23
1215
PRIMER_RIGHT_13=203,20
1216
PRIMER_LEFT_13_TM=58.486
1217
PRIMER_RIGHT_13_TM=60.061
1218
PRIMER_LEFT_13_GC_PERCENT=47.826
1219
PRIMER_RIGHT_13_GC_PERCENT=55.000
1220
PRIMER_LEFT_13_SELF_ANY=1.00
1221
PRIMER_RIGHT_13_SELF_ANY=2.00
1222
PRIMER_LEFT_13_SELF_END=1.00
1223
PRIMER_RIGHT_13_SELF_END=0.00
1224
PRIMER_LEFT_13_END_STABILITY=6.7000
1225
PRIMER_RIGHT_13_END_STABILITY=5.9000
1226
PRIMER_PAIR_13_COMPL_ANY=3.00
1227
PRIMER_PAIR_13_COMPL_END=2.00
1228
PRIMER_PRODUCT_SIZE_13=168
1229
PRIMER_PAIR_PENALTY_14=4.6705
1230
PRIMER_LEFT_14_PENALTY=4.609236
1231
PRIMER_RIGHT_14_PENALTY=0.061230
1232
PRIMER_LEFT_14_SEQUENCE=ACACAAACAGAGACACAGACACAG
1233
PRIMER_RIGHT_14_SEQUENCE=GCTTTCCCGCTACCCTATTC
1234
PRIMER_LEFT_14=35,24
1235
PRIMER_RIGHT_14=203,20
1236
PRIMER_LEFT_14_TM=59.391
1237
PRIMER_RIGHT_14_TM=60.061
1238
PRIMER_LEFT_14_GC_PERCENT=45.833
1239
PRIMER_RIGHT_14_GC_PERCENT=55.000
1240
PRIMER_LEFT_14_SELF_ANY=1.00
1241
PRIMER_RIGHT_14_SELF_ANY=2.00
1242
PRIMER_LEFT_14_SELF_END=1.00
1243
PRIMER_RIGHT_14_SELF_END=0.00
1244
PRIMER_LEFT_14_END_STABILITY=6.7000
1245
PRIMER_RIGHT_14_END_STABILITY=5.9000
1246
PRIMER_PAIR_14_COMPL_ANY=3.00
1247
PRIMER_PAIR_14_COMPL_END=2.00
1248
PRIMER_PRODUCT_SIZE_14=169
1249
PRIMER_PAIR_PENALTY_15=4.6843
1250
PRIMER_LEFT_15_PENALTY=4.669014
1251
PRIMER_RIGHT_15_PENALTY=0.015253
1252
PRIMER_LEFT_15_SEQUENCE=CACAAACAGAGACACAGACACA
1253
PRIMER_RIGHT_15_SEQUENCE=CTTCTGTGCTTTCCCGCTAC
1254
PRIMER_LEFT_15=36,22
1255
PRIMER_RIGHT_15=210,20
1256
PRIMER_LEFT_15_TM=57.331
1257
PRIMER_RIGHT_15_TM=60.015
1258
PRIMER_LEFT_15_GC_PERCENT=45.455
1259
PRIMER_RIGHT_15_GC_PERCENT=55.000
1260
PRIMER_LEFT_15_SELF_ANY=1.00
1261
PRIMER_RIGHT_15_SELF_ANY=2.00
1262
PRIMER_LEFT_15_SELF_END=0.00
1263
PRIMER_RIGHT_15_SELF_END=0.00
1264
PRIMER_LEFT_15_END_STABILITY=6.4000
1265
PRIMER_RIGHT_15_END_STABILITY=6.9000
1266
PRIMER_PAIR_15_COMPL_ANY=6.00
1267
PRIMER_PAIR_15_COMPL_END=2.00
1268
PRIMER_PRODUCT_SIZE_15=175
1269
PRIMER_PAIR_PENALTY_16=4.7302
1270
PRIMER_LEFT_16_PENALTY=4.669014
1271
PRIMER_RIGHT_16_PENALTY=0.061230
1272
PRIMER_LEFT_16_SEQUENCE=CACAAACAGAGACACAGACACA
1273
PRIMER_RIGHT_16_SEQUENCE=GCTTTCCCGCTACCCTATTC
1274
PRIMER_LEFT_16=36,22
1275
PRIMER_RIGHT_16=203,20
1276
PRIMER_LEFT_16_TM=57.331
1277
PRIMER_RIGHT_16_TM=60.061
1278
PRIMER_LEFT_16_GC_PERCENT=45.455
1279
PRIMER_RIGHT_16_GC_PERCENT=55.000
1280
PRIMER_LEFT_16_SELF_ANY=1.00
1281
PRIMER_RIGHT_16_SELF_ANY=2.00
1282
PRIMER_LEFT_16_SELF_END=0.00
1283
PRIMER_RIGHT_16_SELF_END=0.00
1284
PRIMER_LEFT_16_END_STABILITY=6.4000
1285
PRIMER_RIGHT_16_END_STABILITY=5.9000
1286
PRIMER_PAIR_16_COMPL_ANY=3.00
1287
PRIMER_PAIR_16_COMPL_END=1.00
1288
PRIMER_PRODUCT_SIZE_16=168
1289
PRIMER_PAIR_PENALTY_17=4.7302
1290
PRIMER_LEFT_17_PENALTY=4.669014
1291
PRIMER_RIGHT_17_PENALTY=0.061230
1292
PRIMER_LEFT_17_SEQUENCE=CACACAAACAGAGACACAGACA
1293
PRIMER_RIGHT_17_SEQUENCE=GCTTTCCCGCTACCCTATTC
1294
PRIMER_LEFT_17=34,22
1295
PRIMER_RIGHT_17=203,20
1296
PRIMER_LEFT_17_TM=57.331
1297
PRIMER_RIGHT_17_TM=60.061
1298
PRIMER_LEFT_17_GC_PERCENT=45.455
1299
PRIMER_RIGHT_17_GC_PERCENT=55.000
1300
PRIMER_LEFT_17_SELF_ANY=1.00
1301
PRIMER_RIGHT_17_SELF_ANY=2.00
1302
PRIMER_LEFT_17_SELF_END=0.00
1303
PRIMER_RIGHT_17_SELF_END=0.00
1304
PRIMER_LEFT_17_END_STABILITY=6.4000
1305
PRIMER_RIGHT_17_END_STABILITY=5.9000
1306
PRIMER_PAIR_17_COMPL_ANY=3.00
1307
PRIMER_PAIR_17_COMPL_END=1.00
1308
PRIMER_PRODUCT_SIZE_17=170
1309
PRIMER_PAIR_PENALTY_18=4.7302
1310
PRIMER_LEFT_18_PENALTY=4.669014
1311
PRIMER_RIGHT_18_PENALTY=0.061230
1312
PRIMER_LEFT_18_SEQUENCE=CACACACAAACAGAGACACAGA
1313
PRIMER_RIGHT_18_SEQUENCE=GCTTTCCCGCTACCCTATTC
1314
PRIMER_LEFT_18=32,22
1315
PRIMER_RIGHT_18=203,20
1316
PRIMER_LEFT_18_TM=57.331
1317
PRIMER_RIGHT_18_TM=60.061
1318
PRIMER_LEFT_18_GC_PERCENT=45.455
1319
PRIMER_RIGHT_18_GC_PERCENT=55.000
1320
PRIMER_LEFT_18_SELF_ANY=1.00
1321
PRIMER_RIGHT_18_SELF_ANY=2.00
1322
PRIMER_LEFT_18_SELF_END=0.00
1323
PRIMER_RIGHT_18_SELF_END=0.00
1324
PRIMER_LEFT_18_END_STABILITY=6.4000
1325
PRIMER_RIGHT_18_END_STABILITY=5.9000
1326
PRIMER_PAIR_18_COMPL_ANY=3.00
1327
PRIMER_PAIR_18_COMPL_END=2.00
1328
PRIMER_PRODUCT_SIZE_18=172
1329
PRIMER_PAIR_PENALTY_19=4.7308
1330
PRIMER_LEFT_19_PENALTY=4.669525
1331
PRIMER_RIGHT_19_PENALTY=0.061230
1332
PRIMER_LEFT_19_SEQUENCE=CACACAAACAGAGACACAGACAC
1333
PRIMER_RIGHT_19_SEQUENCE=GCTTTCCCGCTACCCTATTC
1334
PRIMER_LEFT_19=34,23
1335
PRIMER_RIGHT_19=203,20
1336
PRIMER_LEFT_19_TM=58.330
1337
PRIMER_RIGHT_19_TM=60.061
1338
PRIMER_LEFT_19_GC_PERCENT=47.826
1339
PRIMER_RIGHT_19_GC_PERCENT=55.000
1340
PRIMER_LEFT_19_SELF_ANY=1.00
1341
PRIMER_RIGHT_19_SELF_ANY=2.00
1342
PRIMER_LEFT_19_SELF_END=0.00
1343
PRIMER_RIGHT_19_SELF_END=0.00
1344
PRIMER_LEFT_19_END_STABILITY=6.1000
1345
PRIMER_RIGHT_19_END_STABILITY=5.9000
1346
PRIMER_PAIR_19_COMPL_ANY=3.00
1347
PRIMER_PAIR_19_COMPL_END=0.00
1348
PRIMER_PRODUCT_SIZE_19=170
1349
PRIMER_PAIR_PENALTY_20=4.7308
1350
PRIMER_LEFT_20_PENALTY=4.669525
1351
PRIMER_RIGHT_20_PENALTY=0.061230
1352
PRIMER_LEFT_20_SEQUENCE=ACACAAACAGAGACACAGACACA
1353
PRIMER_RIGHT_20_SEQUENCE=GCTTTCCCGCTACCCTATTC
1354
PRIMER_LEFT_20=35,23
1355
PRIMER_RIGHT_20=203,20
1356
PRIMER_LEFT_20_TM=58.330
1357
PRIMER_RIGHT_20_TM=60.061
1358
PRIMER_LEFT_20_GC_PERCENT=43.478
1359
PRIMER_RIGHT_20_GC_PERCENT=55.000
1360
PRIMER_LEFT_20_SELF_ANY=1.00
1361
PRIMER_RIGHT_20_SELF_ANY=2.00
1362
PRIMER_LEFT_20_SELF_END=0.00
1363
PRIMER_RIGHT_20_SELF_END=0.00
1364
PRIMER_LEFT_20_END_STABILITY=6.4000
1365
PRIMER_RIGHT_20_END_STABILITY=5.9000
1366
PRIMER_PAIR_20_COMPL_ANY=3.00
1367
PRIMER_PAIR_20_COMPL_END=1.00
1368
PRIMER_PRODUCT_SIZE_20=169
1369
PRIMER_PAIR_PENALTY_21=4.7308
1370
PRIMER_LEFT_21_PENALTY=4.669525
1371
PRIMER_RIGHT_21_PENALTY=0.061230
1372
PRIMER_LEFT_21_SEQUENCE=ACACACAAACAGAGACACAGACA
1373
PRIMER_RIGHT_21_SEQUENCE=GCTTTCCCGCTACCCTATTC
1374
PRIMER_LEFT_21=33,23
1375
PRIMER_RIGHT_21=203,20
1376
PRIMER_LEFT_21_TM=58.330
1377
PRIMER_RIGHT_21_TM=60.061
1378
PRIMER_LEFT_21_GC_PERCENT=43.478
1379
PRIMER_RIGHT_21_GC_PERCENT=55.000
1380
PRIMER_LEFT_21_SELF_ANY=1.00
1381
PRIMER_RIGHT_21_SELF_ANY=2.00
1382
PRIMER_LEFT_21_SELF_END=0.00
1383
PRIMER_RIGHT_21_SELF_END=0.00
1384
PRIMER_LEFT_21_END_STABILITY=6.4000
1385
PRIMER_RIGHT_21_END_STABILITY=5.9000
1386
PRIMER_PAIR_21_COMPL_ANY=3.00
1387
PRIMER_PAIR_21_COMPL_END=1.00
1388
PRIMER_PRODUCT_SIZE_21=171
1389
PRIMER_PAIR_PENALTY_22=4.7308
1390
PRIMER_LEFT_22_PENALTY=4.669525
1391
PRIMER_RIGHT_22_PENALTY=0.061230
1392
PRIMER_LEFT_22_SEQUENCE=CACACACAAACAGAGACACAGAC
1393
PRIMER_RIGHT_22_SEQUENCE=GCTTTCCCGCTACCCTATTC
1394
PRIMER_LEFT_22=32,23
1395
PRIMER_RIGHT_22=203,20
1396
PRIMER_LEFT_22_TM=58.330
1397
PRIMER_RIGHT_22_TM=60.061
1398
PRIMER_LEFT_22_GC_PERCENT=47.826
1399
PRIMER_RIGHT_22_GC_PERCENT=55.000
1400
PRIMER_LEFT_22_SELF_ANY=1.00
1401
PRIMER_RIGHT_22_SELF_ANY=2.00
1402
PRIMER_LEFT_22_SELF_END=1.00
1403
PRIMER_RIGHT_22_SELF_END=0.00
1404
PRIMER_LEFT_22_END_STABILITY=6.4000
1405
PRIMER_RIGHT_22_END_STABILITY=5.9000
1406
PRIMER_PAIR_22_COMPL_ANY=3.00
1407
PRIMER_PAIR_22_COMPL_END=1.00
1408
PRIMER_PRODUCT_SIZE_22=172
1409
PRIMER_PAIR_PENALTY_23=4.7308
1410
PRIMER_LEFT_23_PENALTY=4.669525
1411
PRIMER_RIGHT_23_PENALTY=0.061230
1412
PRIMER_LEFT_23_SEQUENCE=ACACACACAAACAGAGACACAGA
1413
PRIMER_RIGHT_23_SEQUENCE=GCTTTCCCGCTACCCTATTC
1414
PRIMER_LEFT_23=31,23
1415
PRIMER_RIGHT_23=203,20
1416
PRIMER_LEFT_23_TM=58.330
1417
PRIMER_RIGHT_23_TM=60.061
1418
PRIMER_LEFT_23_GC_PERCENT=43.478
1419
PRIMER_RIGHT_23_GC_PERCENT=55.000
1420
PRIMER_LEFT_23_SELF_ANY=1.00
1421
PRIMER_RIGHT_23_SELF_ANY=2.00
1422
PRIMER_LEFT_23_SELF_END=0.00
1423
PRIMER_RIGHT_23_SELF_END=0.00
1424
PRIMER_LEFT_23_END_STABILITY=6.4000
1425
PRIMER_RIGHT_23_END_STABILITY=5.9000
1426
PRIMER_PAIR_23_COMPL_ANY=3.00
1427
PRIMER_PAIR_23_COMPL_END=2.00
1428
PRIMER_PRODUCT_SIZE_23=173
1429
PRIMER_PAIR_PENALTY_24=4.8123
1430
PRIMER_LEFT_24_PENALTY=4.751110
1431
PRIMER_RIGHT_24_PENALTY=0.061230
1432
PRIMER_LEFT_24_SEQUENCE=ACACACAAACAGAGACACAGACAC
1433
PRIMER_RIGHT_24_SEQUENCE=GCTTTCCCGCTACCCTATTC
1434
PRIMER_LEFT_24=33,24
1435
PRIMER_RIGHT_24=203,20
1436
PRIMER_LEFT_24_TM=59.249
1437
PRIMER_RIGHT_24_TM=60.061
1438
PRIMER_LEFT_24_GC_PERCENT=45.833
1439
PRIMER_RIGHT_24_GC_PERCENT=55.000
1440
PRIMER_LEFT_24_SELF_ANY=1.00
1441
PRIMER_RIGHT_24_SELF_ANY=2.00
1442
PRIMER_LEFT_24_SELF_END=0.00
1443
PRIMER_RIGHT_24_SELF_END=0.00
1444
PRIMER_LEFT_24_END_STABILITY=6.1000
1445
PRIMER_RIGHT_24_END_STABILITY=5.9000
1446
PRIMER_PAIR_24_COMPL_ANY=3.00
1447
PRIMER_PAIR_24_COMPL_END=0.00
1448
PRIMER_PRODUCT_SIZE_24=171
1449
PRIMER_PAIR_PENALTY_25=4.8123
1450
PRIMER_LEFT_25_PENALTY=4.751110
1451
PRIMER_RIGHT_25_PENALTY=0.061230
1452
PRIMER_LEFT_25_SEQUENCE=ACACACACAAACAGAGACACAGAC
1453
PRIMER_RIGHT_25_SEQUENCE=GCTTTCCCGCTACCCTATTC
1454
PRIMER_LEFT_25=31,24
1455
PRIMER_RIGHT_25=203,20
1456
PRIMER_LEFT_25_TM=59.249
1457
PRIMER_RIGHT_25_TM=60.061
1458
PRIMER_LEFT_25_GC_PERCENT=45.833
1459
PRIMER_RIGHT_25_GC_PERCENT=55.000
1460
PRIMER_LEFT_25_SELF_ANY=1.00
1461
PRIMER_RIGHT_25_SELF_ANY=2.00
1462
PRIMER_LEFT_25_SELF_END=1.00
1463
PRIMER_RIGHT_25_SELF_END=0.00
1464
PRIMER_LEFT_25_END_STABILITY=6.4000
1465
PRIMER_RIGHT_25_END_STABILITY=5.9000
1466
PRIMER_PAIR_25_COMPL_ANY=3.00
1467
PRIMER_PAIR_25_COMPL_END=1.00
1468
PRIMER_PRODUCT_SIZE_25=173
1469
PRIMER_PAIR_PENALTY_26=4.9077
1470
PRIMER_LEFT_26_PENALTY=4.436430
1471
PRIMER_RIGHT_26_PENALTY=0.471231
1472
PRIMER_LEFT_26_SEQUENCE=CACAAACAGAGACACAGACACAGA
1473
PRIMER_RIGHT_26_SEQUENCE=GTGCTTTCCCGCTACCCTAT
1474
PRIMER_LEFT_26=36,24
1475
PRIMER_RIGHT_26=205,20
1476
PRIMER_LEFT_26_TM=60.436
1477
PRIMER_RIGHT_26_TM=60.471
1478
PRIMER_LEFT_26_GC_PERCENT=45.833
1479
PRIMER_RIGHT_26_GC_PERCENT=55.000
1480
PRIMER_LEFT_26_SELF_ANY=1.00
1481
PRIMER_RIGHT_26_SELF_ANY=2.00
1482
PRIMER_LEFT_26_SELF_END=0.00
1483
PRIMER_RIGHT_26_SELF_END=2.00
1484
PRIMER_LEFT_26_END_STABILITY=6.4000
1485
PRIMER_RIGHT_26_END_STABILITY=7.1000
1486
PRIMER_PAIR_26_COMPL_ANY=3.00
1487
PRIMER_PAIR_26_COMPL_END=1.00
1488
PRIMER_PRODUCT_SIZE_26=170
1489
PRIMER_PAIR_PENALTY_27=4.9851
1490
PRIMER_LEFT_27_PENALTY=4.513917
1491
PRIMER_RIGHT_27_PENALTY=0.471231
1492
PRIMER_LEFT_27_SEQUENCE=CACAAACAGAGACACAGACACAG
1493
PRIMER_RIGHT_27_SEQUENCE=GTGCTTTCCCGCTACCCTAT
1494
PRIMER_LEFT_27=36,23
1495
PRIMER_RIGHT_27=205,20
1496
PRIMER_LEFT_27_TM=58.486
1497
PRIMER_RIGHT_27_TM=60.471
1498
PRIMER_LEFT_27_GC_PERCENT=47.826
1499
PRIMER_RIGHT_27_GC_PERCENT=55.000
1500
PRIMER_LEFT_27_SELF_ANY=1.00
1501
PRIMER_RIGHT_27_SELF_ANY=2.00
1502
PRIMER_LEFT_27_SELF_END=1.00
1503
PRIMER_RIGHT_27_SELF_END=2.00
1504
PRIMER_LEFT_27_END_STABILITY=6.7000
1505
PRIMER_RIGHT_27_END_STABILITY=7.1000
1506
PRIMER_PAIR_27_COMPL_ANY=3.00
1507
PRIMER_PAIR_27_COMPL_END=2.00
1508
PRIMER_PRODUCT_SIZE_27=170
1509
PRIMER_PAIR_PENALTY_28=5.0805
1510
PRIMER_LEFT_28_PENALTY=4.609236
1511
PRIMER_RIGHT_28_PENALTY=0.471231
1512
PRIMER_LEFT_28_SEQUENCE=ACACAAACAGAGACACAGACACAG
1513
PRIMER_RIGHT_28_SEQUENCE=GTGCTTTCCCGCTACCCTAT
1514
PRIMER_LEFT_28=35,24
1515
PRIMER_RIGHT_28=205,20
1516
PRIMER_LEFT_28_TM=59.391
1517
PRIMER_RIGHT_28_TM=60.471
1518
PRIMER_LEFT_28_GC_PERCENT=45.833
1519
PRIMER_RIGHT_28_GC_PERCENT=55.000
1520
PRIMER_LEFT_28_SELF_ANY=1.00
1521
PRIMER_RIGHT_28_SELF_ANY=2.00
1522
PRIMER_LEFT_28_SELF_END=1.00
1523
PRIMER_RIGHT_28_SELF_END=2.00
1524
PRIMER_LEFT_28_END_STABILITY=6.7000
1525
PRIMER_RIGHT_28_END_STABILITY=7.1000
1526
PRIMER_PAIR_28_COMPL_ANY=3.00
1527
PRIMER_PAIR_28_COMPL_END=2.00
1528
PRIMER_PRODUCT_SIZE_28=171
1529
PRIMER_PAIR_PENALTY_29=5.0958
1530
PRIMER_LEFT_29_PENALTY=1.153194
1531
PRIMER_RIGHT_29_PENALTY=3.942558
1532
PRIMER_LEFT_29_SEQUENCE=CAACCCAAAAATACAGACGGA
1533
PRIMER_RIGHT_29_SEQUENCE=CCTGTGTTACCCGTCTCAA
1534
PRIMER_LEFT_29=9,21
1535
PRIMER_RIGHT_29=147,19
1536
PRIMER_LEFT_29_TM=59.847
1537
PRIMER_RIGHT_29_TM=57.057
1538
PRIMER_LEFT_29_GC_PERCENT=42.857
1539
PRIMER_RIGHT_29_GC_PERCENT=52.632
1540
PRIMER_LEFT_29_SELF_ANY=2.00
1541
PRIMER_RIGHT_29_SELF_ANY=3.00
1542
PRIMER_LEFT_29_SELF_END=0.00
1543
PRIMER_RIGHT_29_SELF_END=0.00
1544
PRIMER_LEFT_29_END_STABILITY=9.6000
1545
PRIMER_RIGHT_29_END_STABILITY=7.0000
1546
PRIMER_PAIR_29_COMPL_ANY=6.00
1547
PRIMER_PAIR_29_COMPL_END=4.00
1548
PRIMER_PRODUCT_SIZE_29=139
1549
PRIMER_PAIR_PENALTY_30=5.1402
1550
PRIMER_LEFT_30_PENALTY=4.669014
1551
PRIMER_RIGHT_30_PENALTY=0.471231
1552
PRIMER_LEFT_30_SEQUENCE=CACAAACAGAGACACAGACACA
1553
PRIMER_RIGHT_30_SEQUENCE=GTGCTTTCCCGCTACCCTAT
1554
PRIMER_LEFT_30=36,22
1555
PRIMER_RIGHT_30=205,20
1556
PRIMER_LEFT_30_TM=57.331
1557
PRIMER_RIGHT_30_TM=60.471
1558
PRIMER_LEFT_30_GC_PERCENT=45.455
1559
PRIMER_RIGHT_30_GC_PERCENT=55.000
1560
PRIMER_LEFT_30_SELF_ANY=1.00
1561
PRIMER_RIGHT_30_SELF_ANY=2.00
1562
PRIMER_LEFT_30_SELF_END=0.00
1563
PRIMER_RIGHT_30_SELF_END=2.00
1564
PRIMER_LEFT_30_END_STABILITY=6.4000
1565
PRIMER_RIGHT_30_END_STABILITY=7.1000
1566
PRIMER_PAIR_30_COMPL_ANY=3.00
1567
PRIMER_PAIR_30_COMPL_END=1.00
1568
PRIMER_PRODUCT_SIZE_30=170
1569
PRIMER_PAIR_PENALTY_31=5.1402
1570
PRIMER_LEFT_31_PENALTY=4.669014
1571
PRIMER_RIGHT_31_PENALTY=0.471231
1572
PRIMER_LEFT_31_SEQUENCE=CACACAAACAGAGACACAGACA
1573
PRIMER_RIGHT_31_SEQUENCE=GTGCTTTCCCGCTACCCTAT
1574
PRIMER_LEFT_31=34,22
1575
PRIMER_RIGHT_31=205,20
1576
PRIMER_LEFT_31_TM=57.331
1577
PRIMER_RIGHT_31_TM=60.471
1578
PRIMER_LEFT_31_GC_PERCENT=45.455
1579
PRIMER_RIGHT_31_GC_PERCENT=55.000
1580
PRIMER_LEFT_31_SELF_ANY=1.00
1581
PRIMER_RIGHT_31_SELF_ANY=2.00
1582
PRIMER_LEFT_31_SELF_END=0.00
1583
PRIMER_RIGHT_31_SELF_END=2.00
1584
PRIMER_LEFT_31_END_STABILITY=6.4000
1585
PRIMER_RIGHT_31_END_STABILITY=7.1000
1586
PRIMER_PAIR_31_COMPL_ANY=3.00
1587
PRIMER_PAIR_31_COMPL_END=1.00
1588
PRIMER_PRODUCT_SIZE_31=172
1589
PRIMER_PAIR_PENALTY_32=5.1402
1590
PRIMER_LEFT_32_PENALTY=4.669014
1591
PRIMER_RIGHT_32_PENALTY=0.471231
1592
PRIMER_LEFT_32_SEQUENCE=CACACACAAACAGAGACACAGA
1593
PRIMER_RIGHT_32_SEQUENCE=GTGCTTTCCCGCTACCCTAT
1594
PRIMER_LEFT_32=32,22
1595
PRIMER_RIGHT_32=205,20
1596
PRIMER_LEFT_32_TM=57.331
1597
PRIMER_RIGHT_32_TM=60.471
1598
PRIMER_LEFT_32_GC_PERCENT=45.455
1599
PRIMER_RIGHT_32_GC_PERCENT=55.000
1600
PRIMER_LEFT_32_SELF_ANY=1.00
1601
PRIMER_RIGHT_32_SELF_ANY=2.00
1602
PRIMER_LEFT_32_SELF_END=0.00
1603
PRIMER_RIGHT_32_SELF_END=2.00
1604
PRIMER_LEFT_32_END_STABILITY=6.4000
1605
PRIMER_RIGHT_32_END_STABILITY=7.1000
1606
PRIMER_PAIR_32_COMPL_ANY=3.00
1607
PRIMER_PAIR_32_COMPL_END=1.00
1608
PRIMER_PRODUCT_SIZE_32=174
1609
PRIMER_PAIR_PENALTY_33=5.1408
1610
PRIMER_LEFT_33_PENALTY=4.669525
1611
PRIMER_RIGHT_33_PENALTY=0.471231
1612
PRIMER_LEFT_33_SEQUENCE=CACACAAACAGAGACACAGACAC
1613
PRIMER_RIGHT_33_SEQUENCE=GTGCTTTCCCGCTACCCTAT
1614
PRIMER_LEFT_33=34,23
1615
PRIMER_RIGHT_33=205,20
1616
PRIMER_LEFT_33_TM=58.330
1617
PRIMER_RIGHT_33_TM=60.471
1618
PRIMER_LEFT_33_GC_PERCENT=47.826
1619
PRIMER_RIGHT_33_GC_PERCENT=55.000
1620
PRIMER_LEFT_33_SELF_ANY=1.00
1621
PRIMER_RIGHT_33_SELF_ANY=2.00
1622
PRIMER_LEFT_33_SELF_END=0.00
1623
PRIMER_RIGHT_33_SELF_END=2.00
1624
PRIMER_LEFT_33_END_STABILITY=6.1000
1625
PRIMER_RIGHT_33_END_STABILITY=7.1000
1626
PRIMER_PAIR_33_COMPL_ANY=3.00
1627
PRIMER_PAIR_33_COMPL_END=0.00
1628
PRIMER_PRODUCT_SIZE_33=172
1629
PRIMER_PAIR_PENALTY_34=5.1408
1630
PRIMER_LEFT_34_PENALTY=4.669525
1631
PRIMER_RIGHT_34_PENALTY=0.471231
1632
PRIMER_LEFT_34_SEQUENCE=ACACAAACAGAGACACAGACACA
1633
PRIMER_RIGHT_34_SEQUENCE=GTGCTTTCCCGCTACCCTAT
1634
PRIMER_LEFT_34=35,23
1635
PRIMER_RIGHT_34=205,20
1636
PRIMER_LEFT_34_TM=58.330
1637
PRIMER_RIGHT_34_TM=60.471
1638
PRIMER_LEFT_34_GC_PERCENT=43.478
1639
PRIMER_RIGHT_34_GC_PERCENT=55.000
1640
PRIMER_LEFT_34_SELF_ANY=1.00
1641
PRIMER_RIGHT_34_SELF_ANY=2.00
1642
PRIMER_LEFT_34_SELF_END=0.00
1643
PRIMER_RIGHT_34_SELF_END=2.00
1644
PRIMER_LEFT_34_END_STABILITY=6.4000
1645
PRIMER_RIGHT_34_END_STABILITY=7.1000
1646
PRIMER_PAIR_34_COMPL_ANY=3.00
1647
PRIMER_PAIR_34_COMPL_END=1.00
1648
PRIMER_PRODUCT_SIZE_34=171
1649
PRIMER_PAIR_PENALTY_35=5.1408
1650
PRIMER_LEFT_35_PENALTY=4.669525
1651
PRIMER_RIGHT_35_PENALTY=0.471231
1652
PRIMER_LEFT_35_SEQUENCE=ACACACAAACAGAGACACAGACA
1653
PRIMER_RIGHT_35_SEQUENCE=GTGCTTTCCCGCTACCCTAT
1654
PRIMER_LEFT_35=33,23
1655
PRIMER_RIGHT_35=205,20
1656
PRIMER_LEFT_35_TM=58.330
1657
PRIMER_RIGHT_35_TM=60.471
1658
PRIMER_LEFT_35_GC_PERCENT=43.478
1659
PRIMER_RIGHT_35_GC_PERCENT=55.000
1660
PRIMER_LEFT_35_SELF_ANY=1.00
1661
PRIMER_RIGHT_35_SELF_ANY=2.00
1662
PRIMER_LEFT_35_SELF_END=0.00
1663
PRIMER_RIGHT_35_SELF_END=2.00
1664
PRIMER_LEFT_35_END_STABILITY=6.4000
1665
PRIMER_RIGHT_35_END_STABILITY=7.1000
1666
PRIMER_PAIR_35_COMPL_ANY=3.00
1667
PRIMER_PAIR_35_COMPL_END=1.00
1668
PRIMER_PRODUCT_SIZE_35=173
1669
PRIMER_PAIR_PENALTY_36=5.1408
1670
PRIMER_LEFT_36_PENALTY=4.669525
1671
PRIMER_RIGHT_36_PENALTY=0.471231
1672
PRIMER_LEFT_36_SEQUENCE=CACACACAAACAGAGACACAGAC
1673
PRIMER_RIGHT_36_SEQUENCE=GTGCTTTCCCGCTACCCTAT
1674
PRIMER_LEFT_36=32,23
1675
PRIMER_RIGHT_36=205,20
1676
PRIMER_LEFT_36_TM=58.330
1677
PRIMER_RIGHT_36_TM=60.471
1678
PRIMER_LEFT_36_GC_PERCENT=47.826
1679
PRIMER_RIGHT_36_GC_PERCENT=55.000
1680
PRIMER_LEFT_36_SELF_ANY=1.00
1681
PRIMER_RIGHT_36_SELF_ANY=2.00
1682
PRIMER_LEFT_36_SELF_END=1.00
1683
PRIMER_RIGHT_36_SELF_END=2.00
1684
PRIMER_LEFT_36_END_STABILITY=6.4000
1685
PRIMER_RIGHT_36_END_STABILITY=7.1000
1686
PRIMER_PAIR_36_COMPL_ANY=3.00
1687
PRIMER_PAIR_36_COMPL_END=0.00
1688
PRIMER_PRODUCT_SIZE_36=174
1689
PRIMER_PAIR_PENALTY_37=5.1408
1690
PRIMER_LEFT_37_PENALTY=4.669525
1691
PRIMER_RIGHT_37_PENALTY=0.471231
1692
PRIMER_LEFT_37_SEQUENCE=ACACACACAAACAGAGACACAGA
1693
PRIMER_RIGHT_37_SEQUENCE=GTGCTTTCCCGCTACCCTAT
1694
PRIMER_LEFT_37=31,23
1695
PRIMER_RIGHT_37=205,20
1696
PRIMER_LEFT_37_TM=58.330
1697
PRIMER_RIGHT_37_TM=60.471
1698
PRIMER_LEFT_37_GC_PERCENT=43.478
1699
PRIMER_RIGHT_37_GC_PERCENT=55.000
1700
PRIMER_LEFT_37_SELF_ANY=1.00
1701
PRIMER_RIGHT_37_SELF_ANY=2.00
1702
PRIMER_LEFT_37_SELF_END=0.00
1703
PRIMER_RIGHT_37_SELF_END=2.00
1704
PRIMER_LEFT_37_END_STABILITY=6.4000
1705
PRIMER_RIGHT_37_END_STABILITY=7.1000
1706
PRIMER_PAIR_37_COMPL_ANY=3.00
1707
PRIMER_PAIR_37_COMPL_END=1.00
1708
PRIMER_PRODUCT_SIZE_37=175
1709
PRIMER_PAIR_PENALTY_38=5.2223
1710
PRIMER_LEFT_38_PENALTY=4.751110
1711
PRIMER_RIGHT_38_PENALTY=0.471231
1712
PRIMER_LEFT_38_SEQUENCE=ACACACAAACAGAGACACAGACAC
1713
PRIMER_RIGHT_38_SEQUENCE=GTGCTTTCCCGCTACCCTAT
1714
PRIMER_LEFT_38=33,24
1715
PRIMER_RIGHT_38=205,20
1716
PRIMER_LEFT_38_TM=59.249
1717
PRIMER_RIGHT_38_TM=60.471
1718
PRIMER_LEFT_38_GC_PERCENT=45.833
1719
PRIMER_RIGHT_38_GC_PERCENT=55.000
1720
PRIMER_LEFT_38_SELF_ANY=1.00
1721
PRIMER_RIGHT_38_SELF_ANY=2.00
1722
PRIMER_LEFT_38_SELF_END=0.00
1723
PRIMER_RIGHT_38_SELF_END=2.00
1724
PRIMER_LEFT_38_END_STABILITY=6.1000
1725
PRIMER_RIGHT_38_END_STABILITY=7.1000
1726
PRIMER_PAIR_38_COMPL_ANY=3.00
1727
PRIMER_PAIR_38_COMPL_END=0.00
1728
PRIMER_PRODUCT_SIZE_38=173
1729
PRIMER_PAIR_PENALTY_39=5.2223
1730
PRIMER_LEFT_39_PENALTY=4.751110
1731
PRIMER_RIGHT_39_PENALTY=0.471231
1732
PRIMER_LEFT_39_SEQUENCE=ACACACACAAACAGAGACACAGAC
1733
PRIMER_RIGHT_39_SEQUENCE=GTGCTTTCCCGCTACCCTAT
1734
PRIMER_LEFT_39=31,24
1735
PRIMER_RIGHT_39=205,20
1736
PRIMER_LEFT_39_TM=59.249
1737
PRIMER_RIGHT_39_TM=60.471
1738
PRIMER_LEFT_39_GC_PERCENT=45.833
1739
PRIMER_RIGHT_39_GC_PERCENT=55.000
1740
PRIMER_LEFT_39_SELF_ANY=1.00
1741
PRIMER_RIGHT_39_SELF_ANY=2.00
1742
PRIMER_LEFT_39_SELF_END=1.00
1743
PRIMER_RIGHT_39_SELF_END=2.00
1744
PRIMER_LEFT_39_END_STABILITY=6.4000
1745
PRIMER_RIGHT_39_END_STABILITY=7.1000
1746
PRIMER_PAIR_39_COMPL_ANY=3.00
1747
PRIMER_PAIR_39_COMPL_END=0.00
1748
PRIMER_PRODUCT_SIZE_39=175
1749
=
1750
MARKER_NAME=07.RHAP34FE7.seq
1751
SEQUENCE=NNNNNNNNNNNNNNNNNNCCAGGACTGGAGTTCTCAACTGGAAGCAGGGCATAATAAATATACACAGACTACTCTTTGGGTACAAACTGACAGGCAATTTCAAGGCTTAAAGTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCTCATGCACACCCCCCCTCTCCACCTGAACCTCTACATGCGTGCACATGTTCACACATGTTCTCTCATTTGCTCTCACACTTGCTCTCATTACTCGCTCTCTAGACCTT
1752
INCLUDED_REGION=18,283
1753
TARGET=115,29,CT
1754
TARGET=145,20,NG
1755
TARGET=166,24,CA
1756
PRIMER_PAIR_PENALTY=0.4846
1757
PRIMER_LEFT_PENALTY=0.151542
1758
PRIMER_RIGHT_PENALTY=0.333053
1759
PRIMER_LEFT_SEQUENCE=TGGGTACAAACTGACAGGCA
1760
PRIMER_RIGHT_SEQUENCE=TGTGCACGCATGTAGAGGTT
1761
PRIMER_LEFT=76,20
1762
PRIMER_RIGHT=238,20
1763
PRIMER_LEFT_TM=60.152
1764
PRIMER_RIGHT_TM=60.333
1765
PRIMER_LEFT_GC_PERCENT=50.000
1766
PRIMER_RIGHT_GC_PERCENT=50.000
1767
PRIMER_LEFT_SELF_ANY=5.00
1768
PRIMER_RIGHT_SELF_ANY=6.00
1769
PRIMER_LEFT_SELF_END=3.00
1770
PRIMER_RIGHT_SELF_END=0.00
1771
PRIMER_LEFT_END_STABILITY=9.7000
1772
PRIMER_RIGHT_END_STABILITY=7.9000
1773
PRIMER_PAIR_COMPL_ANY=4.00
1774
PRIMER_PAIR_COMPL_END=3.00
1775
PRIMER_PRODUCT_SIZE=163
1776
PRIMER_PAIR_PENALTY_1=0.6812
1777
PRIMER_LEFT_1_PENALTY=0.151542
1778
PRIMER_RIGHT_1_PENALTY=0.529649
1779
PRIMER_LEFT_1_SEQUENCE=TGGGTACAAACTGACAGGCA
1780
PRIMER_RIGHT_1_SEQUENCE=GCACGCATGTAGAGGTTCAG
1781
PRIMER_LEFT_1=76,20
1782
PRIMER_RIGHT_1=235,20
1783
PRIMER_LEFT_1_TM=60.152
1784
PRIMER_RIGHT_1_TM=59.470
1785
PRIMER_LEFT_1_GC_PERCENT=50.000
1786
PRIMER_RIGHT_1_GC_PERCENT=55.000
1787
PRIMER_LEFT_1_SELF_ANY=5.00
1788
PRIMER_RIGHT_1_SELF_ANY=4.00
1789
PRIMER_LEFT_1_SELF_END=3.00
1790
PRIMER_RIGHT_1_SELF_END=1.00
1791
PRIMER_LEFT_1_END_STABILITY=9.7000
1792
PRIMER_RIGHT_1_END_STABILITY=7.0000
1793
PRIMER_PAIR_1_COMPL_ANY=4.00
1794
PRIMER_PAIR_1_COMPL_END=0.00
1795
PRIMER_PRODUCT_SIZE_1=160
1796
PRIMER_PAIR_PENALTY_2=0.7897
1797
PRIMER_LEFT_2_PENALTY=0.151542
1798
PRIMER_RIGHT_2_PENALTY=0.638191
1799
PRIMER_LEFT_2_SEQUENCE=TGGGTACAAACTGACAGGCA
1800
PRIMER_RIGHT_2_SEQUENCE=AGAGGTTCAGGTGGAGAGGG
1801
PRIMER_LEFT_2=76,20
1802
PRIMER_RIGHT_2=225,20
1803
PRIMER_LEFT_2_TM=60.152
1804
PRIMER_RIGHT_2_TM=60.638
1805
PRIMER_LEFT_2_GC_PERCENT=50.000
1806
PRIMER_RIGHT_2_GC_PERCENT=60.000
1807
PRIMER_LEFT_2_SELF_ANY=5.00
1808
PRIMER_RIGHT_2_SELF_ANY=2.00
1809
PRIMER_LEFT_2_SELF_END=3.00
1810
PRIMER_RIGHT_2_SELF_END=0.00
1811
PRIMER_LEFT_2_END_STABILITY=9.7000
1812
PRIMER_RIGHT_2_END_STABILITY=9.4000
1813
PRIMER_PAIR_2_COMPL_ANY=4.00
1814
PRIMER_PAIR_2_COMPL_END=0.00
1815
PRIMER_PRODUCT_SIZE_2=150
1816
PRIMER_PAIR_PENALTY_3=0.8285
1817
PRIMER_LEFT_3_PENALTY=0.151542
1818
PRIMER_RIGHT_3_PENALTY=0.676947
1819
PRIMER_LEFT_3_SEQUENCE=TGGGTACAAACTGACAGGCA
1820
PRIMER_RIGHT_3_SEQUENCE=GTGCACGCATGTAGAGGTTC
1821
PRIMER_LEFT_3=76,20
1822
PRIMER_RIGHT_3=237,20
1823
PRIMER_LEFT_3_TM=60.152
1824
PRIMER_RIGHT_3_TM=59.323
1825
PRIMER_LEFT_3_GC_PERCENT=50.000
1826
PRIMER_RIGHT_3_GC_PERCENT=55.000
1827
PRIMER_LEFT_3_SELF_ANY=5.00
1828
PRIMER_RIGHT_3_SELF_ANY=6.00
1829
PRIMER_LEFT_3_SELF_END=3.00
1830
PRIMER_RIGHT_3_SELF_END=1.00
1831
PRIMER_LEFT_3_END_STABILITY=9.7000
1832
PRIMER_RIGHT_3_END_STABILITY=7.9000
1833
PRIMER_PAIR_3_COMPL_ANY=4.00
1834
PRIMER_PAIR_3_COMPL_END=2.00
1835
PRIMER_PRODUCT_SIZE_3=162
1836
PRIMER_PAIR_PENALTY_4=0.8378
1837
PRIMER_LEFT_4_PENALTY=0.151542
1838
PRIMER_RIGHT_4_PENALTY=0.686256
1839
PRIMER_LEFT_4_SEQUENCE=TGGGTACAAACTGACAGGCA
1840
PRIMER_RIGHT_4_SEQUENCE=CACGCATGTAGAGGTTCAGG
1841
PRIMER_LEFT_4=76,20
1842
PRIMER_RIGHT_4=234,20
1843
PRIMER_LEFT_4_TM=60.152
1844
PRIMER_RIGHT_4_TM=59.314
1845
PRIMER_LEFT_4_GC_PERCENT=50.000
1846
PRIMER_RIGHT_4_GC_PERCENT=55.000
1847
PRIMER_LEFT_4_SELF_ANY=5.00
1848
PRIMER_RIGHT_4_SELF_ANY=4.00
1849
PRIMER_LEFT_4_SELF_END=3.00
1850
PRIMER_RIGHT_4_SELF_END=0.00
1851
PRIMER_LEFT_4_END_STABILITY=9.7000
1852
PRIMER_RIGHT_4_END_STABILITY=8.2000
1853
PRIMER_PAIR_4_COMPL_ANY=4.00
1854
PRIMER_PAIR_4_COMPL_END=0.00
1855
PRIMER_PRODUCT_SIZE_4=159
1856
PRIMER_PAIR_PENALTY_5=0.8378
1857
PRIMER_LEFT_5_PENALTY=0.151542
1858
PRIMER_RIGHT_5_PENALTY=0.686256
1859
PRIMER_LEFT_5_SEQUENCE=TGGGTACAAACTGACAGGCA
1860
PRIMER_RIGHT_5_SEQUENCE=CGCATGTAGAGGTTCAGGTG
1861
PRIMER_LEFT_5=76,20
1862
PRIMER_RIGHT_5=232,20
1863
PRIMER_LEFT_5_TM=60.152
1864
PRIMER_RIGHT_5_TM=59.314
1865
PRIMER_LEFT_5_GC_PERCENT=50.000
1866
PRIMER_RIGHT_5_GC_PERCENT=55.000
1867
PRIMER_LEFT_5_SELF_ANY=5.00
1868
PRIMER_RIGHT_5_SELF_ANY=4.00
1869
PRIMER_LEFT_5_SELF_END=3.00
1870
PRIMER_RIGHT_5_SELF_END=2.00
1871
PRIMER_LEFT_5_END_STABILITY=9.7000
1872
PRIMER_RIGHT_5_END_STABILITY=7.9000
1873
PRIMER_PAIR_5_COMPL_ANY=4.00
1874
PRIMER_PAIR_5_COMPL_END=2.00
1875
PRIMER_PRODUCT_SIZE_5=157
1876
PRIMER_PAIR_PENALTY_6=0.9180
1877
PRIMER_LEFT_6_PENALTY=0.151542
1878
PRIMER_RIGHT_6_PENALTY=0.766499
1879
PRIMER_LEFT_6_SEQUENCE=TGGGTACAAACTGACAGGCA
1880
PRIMER_RIGHT_6_SEQUENCE=TGAACATGTGCACGCATGTA
1881
PRIMER_LEFT_6=76,20
1882
PRIMER_RIGHT_6=244,20
1883
PRIMER_LEFT_6_TM=60.152
1884
PRIMER_RIGHT_6_TM=60.766
1885
PRIMER_LEFT_6_GC_PERCENT=50.000
1886
PRIMER_RIGHT_6_GC_PERCENT=45.000
1887
PRIMER_LEFT_6_SELF_ANY=5.00
1888
PRIMER_RIGHT_6_SELF_ANY=8.00
1889
PRIMER_LEFT_6_SELF_END=3.00
1890
PRIMER_RIGHT_6_SELF_END=6.00
1891
PRIMER_LEFT_6_END_STABILITY=9.7000
1892
PRIMER_RIGHT_6_END_STABILITY=5.6000
1893
PRIMER_PAIR_6_COMPL_ANY=4.00
1894
PRIMER_PAIR_6_COMPL_END=2.00
1895
PRIMER_PRODUCT_SIZE_6=169
1896
PRIMER_PAIR_PENALTY_7=0.9562
1897
PRIMER_LEFT_7_PENALTY=0.151542
1898
PRIMER_RIGHT_7_PENALTY=0.804687
1899
PRIMER_LEFT_7_SEQUENCE=TGGGTACAAACTGACAGGCA
1900
PRIMER_RIGHT_7_SEQUENCE=ATGTGCACGCATGTAGAGGT
1901
PRIMER_LEFT_7=76,20
1902
PRIMER_RIGHT_7=239,20
1903
PRIMER_LEFT_7_TM=60.152
1904
PRIMER_RIGHT_7_TM=59.195
1905
PRIMER_LEFT_7_GC_PERCENT=50.000
1906
PRIMER_RIGHT_7_GC_PERCENT=50.000
1907
PRIMER_LEFT_7_SELF_ANY=5.00
1908
PRIMER_RIGHT_7_SELF_ANY=6.00
1909
PRIMER_LEFT_7_SELF_END=3.00
1910
PRIMER_RIGHT_7_SELF_END=0.00
1911
PRIMER_LEFT_7_END_STABILITY=9.7000
1912
PRIMER_RIGHT_7_END_STABILITY=7.6000
1913
PRIMER_PAIR_7_COMPL_ANY=4.00
1914
PRIMER_PAIR_7_COMPL_END=2.00
1915
PRIMER_PRODUCT_SIZE_7=164
1916
PRIMER_PAIR_PENALTY_8=1.0167
1917
PRIMER_LEFT_8_PENALTY=0.683672
1918
PRIMER_RIGHT_8_PENALTY=0.333053
1919
PRIMER_LEFT_8_SEQUENCE=GACAGGCAATTTCAAGGCTT
1920
PRIMER_RIGHT_8_SEQUENCE=TGTGCACGCATGTAGAGGTT
1921
PRIMER_LEFT_8=88,20
1922
PRIMER_RIGHT_8=238,20
1923
PRIMER_LEFT_8_TM=59.316
1924
PRIMER_RIGHT_8_TM=60.333
1925
PRIMER_LEFT_8_GC_PERCENT=45.000
1926
PRIMER_RIGHT_8_GC_PERCENT=50.000
1927
PRIMER_LEFT_8_SELF_ANY=5.00
1928
PRIMER_RIGHT_8_SELF_ANY=6.00
1929
PRIMER_LEFT_8_SELF_END=5.00
1930
PRIMER_RIGHT_8_SELF_END=0.00
1931
PRIMER_LEFT_8_END_STABILITY=9.7000
1932
PRIMER_RIGHT_8_END_STABILITY=7.9000
1933
PRIMER_PAIR_8_COMPL_ANY=4.00
1934
PRIMER_PAIR_8_COMPL_END=1.00
1935
PRIMER_PRODUCT_SIZE_8=151
1936
PRIMER_PAIR_PENALTY_9=1.1088
1937
PRIMER_LEFT_9_PENALTY=0.775718
1938
PRIMER_RIGHT_9_PENALTY=0.333053
1939
PRIMER_LEFT_9_SEQUENCE=CTGACAGGCAATTTCAAGGC
1940
PRIMER_RIGHT_9_SEQUENCE=TGTGCACGCATGTAGAGGTT
1941
PRIMER_LEFT_9=86,20
1942
PRIMER_RIGHT_9=238,20
1943
PRIMER_LEFT_9_TM=60.776
1944
PRIMER_RIGHT_9_TM=60.333
1945
PRIMER_LEFT_9_GC_PERCENT=50.000
1946
PRIMER_RIGHT_9_GC_PERCENT=50.000
1947
PRIMER_LEFT_9_SELF_ANY=5.00
1948
PRIMER_RIGHT_9_SELF_ANY=6.00
1949
PRIMER_LEFT_9_SELF_END=2.00
1950
PRIMER_RIGHT_9_SELF_END=0.00
1951
PRIMER_LEFT_9_END_STABILITY=9.7000
1952
PRIMER_RIGHT_9_END_STABILITY=7.9000
1953
PRIMER_PAIR_9_COMPL_ANY=4.00
1954
PRIMER_PAIR_9_COMPL_END=1.00
1955
PRIMER_PRODUCT_SIZE_9=153
1956
PRIMER_PAIR_PENALTY_10=1.1088
1957
PRIMER_LEFT_10_PENALTY=0.775718
1958
PRIMER_RIGHT_10_PENALTY=0.333053
1959
PRIMER_LEFT_10_SEQUENCE=TGACAGGCAATTTCAAGGCT
1960
PRIMER_RIGHT_10_SEQUENCE=TGTGCACGCATGTAGAGGTT
1961
PRIMER_LEFT_10=87,20
1962
PRIMER_RIGHT_10=238,20
1963
PRIMER_LEFT_10_TM=60.776
1964
PRIMER_RIGHT_10_TM=60.333
1965
PRIMER_LEFT_10_GC_PERCENT=45.000
1966
PRIMER_RIGHT_10_GC_PERCENT=50.000
1967
PRIMER_LEFT_10_SELF_ANY=4.00
1968
PRIMER_RIGHT_10_SELF_ANY=6.00
1969
PRIMER_LEFT_10_SELF_END=3.00
1970
PRIMER_RIGHT_10_SELF_END=0.00
1971
PRIMER_LEFT_10_END_STABILITY=9.4000
1972
PRIMER_RIGHT_10_END_STABILITY=7.9000
1973
PRIMER_PAIR_10_COMPL_ANY=4.00
1974
PRIMER_PAIR_10_COMPL_END=1.00
1975
PRIMER_PRODUCT_SIZE_10=152
1976
PRIMER_PAIR_PENALTY_11=1.2133
1977
PRIMER_LEFT_11_PENALTY=0.683672
1978
PRIMER_RIGHT_11_PENALTY=0.529649
1979
PRIMER_LEFT_11_SEQUENCE=GACAGGCAATTTCAAGGCTT
1980
PRIMER_RIGHT_11_SEQUENCE=GCACGCATGTAGAGGTTCAG
1981
PRIMER_LEFT_11=88,20
1982
PRIMER_RIGHT_11=235,20
1983
PRIMER_LEFT_11_TM=59.316
1984
PRIMER_RIGHT_11_TM=59.470
1985
PRIMER_LEFT_11_GC_PERCENT=45.000
1986
PRIMER_RIGHT_11_GC_PERCENT=55.000
1987
PRIMER_LEFT_11_SELF_ANY=5.00
1988
PRIMER_RIGHT_11_SELF_ANY=4.00
1989
PRIMER_LEFT_11_SELF_END=5.00
1990
PRIMER_RIGHT_11_SELF_END=1.00
1991
PRIMER_LEFT_11_END_STABILITY=9.7000
1992
PRIMER_RIGHT_11_END_STABILITY=7.0000
1993
PRIMER_PAIR_11_COMPL_ANY=4.00
1994
PRIMER_PAIR_11_COMPL_END=1.00
1995
PRIMER_PRODUCT_SIZE_11=148
1996
PRIMER_PAIR_PENALTY_12=1.2778
1997
PRIMER_LEFT_12_PENALTY=0.151542
1998
PRIMER_RIGHT_12_PENALTY=1.126283
1999
PRIMER_LEFT_12_SEQUENCE=TGGGTACAAACTGACAGGCA
2000
PRIMER_RIGHT_12_SEQUENCE=GCATGTAGAGGTTCAGGTGGA
2001
PRIMER_LEFT_12=76,20
2002
PRIMER_RIGHT_12=231,21
2003
PRIMER_LEFT_12_TM=60.152
2004
PRIMER_RIGHT_12_TM=60.126
2005
PRIMER_LEFT_12_GC_PERCENT=50.000
2006
PRIMER_RIGHT_12_GC_PERCENT=52.381
2007
PRIMER_LEFT_12_SELF_ANY=5.00
2008
PRIMER_RIGHT_12_SELF_ANY=4.00
2009
PRIMER_LEFT_12_SELF_END=3.00
2010
PRIMER_RIGHT_12_SELF_END=2.00
2011
PRIMER_LEFT_12_END_STABILITY=9.7000
2012
PRIMER_RIGHT_12_END_STABILITY=7.9000
2013
PRIMER_PAIR_12_COMPL_ANY=4.00
2014
PRIMER_PAIR_12_COMPL_END=0.00
2015
PRIMER_PRODUCT_SIZE_12=156
2016
PRIMER_PAIR_PENALTY_13=1.3054
2017
PRIMER_LEFT_13_PENALTY=0.775718
2018
PRIMER_RIGHT_13_PENALTY=0.529649
2019
PRIMER_LEFT_13_SEQUENCE=CTGACAGGCAATTTCAAGGC
2020
PRIMER_RIGHT_13_SEQUENCE=GCACGCATGTAGAGGTTCAG
2021
PRIMER_LEFT_13=86,20
2022
PRIMER_RIGHT_13=235,20
2023
PRIMER_LEFT_13_TM=60.776
2024
PRIMER_RIGHT_13_TM=59.470
2025
PRIMER_LEFT_13_GC_PERCENT=50.000
2026
PRIMER_RIGHT_13_GC_PERCENT=55.000
2027
PRIMER_LEFT_13_SELF_ANY=5.00
2028
PRIMER_RIGHT_13_SELF_ANY=4.00
2029
PRIMER_LEFT_13_SELF_END=2.00
2030
PRIMER_RIGHT_13_SELF_END=1.00
2031
PRIMER_LEFT_13_END_STABILITY=9.7000
2032
PRIMER_RIGHT_13_END_STABILITY=7.0000
2033
PRIMER_PAIR_13_COMPL_ANY=4.00
2034
PRIMER_PAIR_13_COMPL_END=1.00
2035
PRIMER_PRODUCT_SIZE_13=150
2036
PRIMER_PAIR_PENALTY_14=1.3054
2037
PRIMER_LEFT_14_PENALTY=0.775718
2038
PRIMER_RIGHT_14_PENALTY=0.529649
2039
PRIMER_LEFT_14_SEQUENCE=TGACAGGCAATTTCAAGGCT
2040
PRIMER_RIGHT_14_SEQUENCE=GCACGCATGTAGAGGTTCAG
2041
PRIMER_LEFT_14=87,20
2042
PRIMER_RIGHT_14=235,20
2043
PRIMER_LEFT_14_TM=60.776
2044
PRIMER_RIGHT_14_TM=59.470
2045
PRIMER_LEFT_14_GC_PERCENT=45.000
2046
PRIMER_RIGHT_14_GC_PERCENT=55.000
2047
PRIMER_LEFT_14_SELF_ANY=4.00
2048
PRIMER_RIGHT_14_SELF_ANY=4.00
2049
PRIMER_LEFT_14_SELF_END=3.00
2050
PRIMER_RIGHT_14_SELF_END=1.00
2051
PRIMER_LEFT_14_END_STABILITY=9.4000
2052
PRIMER_RIGHT_14_END_STABILITY=7.0000
2053
PRIMER_PAIR_14_COMPL_ANY=4.00
2054
PRIMER_PAIR_14_COMPL_END=2.00
2055
PRIMER_PRODUCT_SIZE_14=149
2056
PRIMER_PAIR_PENALTY_15=1.3219
2057
PRIMER_LEFT_15_PENALTY=0.683672
2058
PRIMER_RIGHT_15_PENALTY=0.638191
2059
PRIMER_LEFT_15_SEQUENCE=GACAGGCAATTTCAAGGCTT
2060
PRIMER_RIGHT_15_SEQUENCE=AGAGGTTCAGGTGGAGAGGG
2061
PRIMER_LEFT_15=88,20
2062
PRIMER_RIGHT_15=225,20
2063
PRIMER_LEFT_15_TM=59.316
2064
PRIMER_RIGHT_15_TM=60.638
2065
PRIMER_LEFT_15_GC_PERCENT=45.000
2066
PRIMER_RIGHT_15_GC_PERCENT=60.000
2067
PRIMER_LEFT_15_SELF_ANY=5.00
2068
PRIMER_RIGHT_15_SELF_ANY=2.00
2069
PRIMER_LEFT_15_SELF_END=5.00
2070
PRIMER_RIGHT_15_SELF_END=0.00
2071
PRIMER_LEFT_15_END_STABILITY=9.7000
2072
PRIMER_RIGHT_15_END_STABILITY=9.4000
2073
PRIMER_PAIR_15_COMPL_ANY=2.00
2074
PRIMER_PAIR_15_COMPL_END=0.00
2075
PRIMER_PRODUCT_SIZE_15=138
2076
PRIMER_PAIR_PENALTY_16=1.3375
2077
PRIMER_LEFT_16_PENALTY=0.151542
2078
PRIMER_RIGHT_16_PENALTY=1.185942
2079
PRIMER_LEFT_16_SEQUENCE=TGGGTACAAACTGACAGGCA
2080
PRIMER_RIGHT_16_SEQUENCE=CACGCATGTAGAGGTTCAGGT
2081
PRIMER_LEFT_16=76,20
2082
PRIMER_RIGHT_16=234,21
2083
PRIMER_LEFT_16_TM=60.152
2084
PRIMER_RIGHT_16_TM=60.186
2085
PRIMER_LEFT_16_GC_PERCENT=50.000
2086
PRIMER_RIGHT_16_GC_PERCENT=52.381
2087
PRIMER_LEFT_16_SELF_ANY=5.00
2088
PRIMER_RIGHT_16_SELF_ANY=4.00
2089
PRIMER_LEFT_16_SELF_END=3.00
2090
PRIMER_RIGHT_16_SELF_END=0.00
2091
PRIMER_LEFT_16_END_STABILITY=9.7000
2092
PRIMER_RIGHT_16_END_STABILITY=7.9000
2093
PRIMER_PAIR_16_COMPL_ANY=4.00
2094
PRIMER_PAIR_16_COMPL_END=1.00
2095
PRIMER_PRODUCT_SIZE_16=159
2096
PRIMER_PAIR_PENALTY_17=1.3375
2097
PRIMER_LEFT_17_PENALTY=0.151542
2098
PRIMER_RIGHT_17_PENALTY=1.185942
2099
PRIMER_LEFT_17_SEQUENCE=TGGGTACAAACTGACAGGCA
2100
PRIMER_RIGHT_17_SEQUENCE=ACGCATGTAGAGGTTCAGGTG
2101
PRIMER_LEFT_17=76,20
2102
PRIMER_RIGHT_17=233,21
2103
PRIMER_LEFT_17_TM=60.152
2104
PRIMER_RIGHT_17_TM=60.186
2105
PRIMER_LEFT_17_GC_PERCENT=50.000
2106
PRIMER_RIGHT_17_GC_PERCENT=52.381
2107
PRIMER_LEFT_17_SELF_ANY=5.00
2108
PRIMER_RIGHT_17_SELF_ANY=4.00
2109
PRIMER_LEFT_17_SELF_END=3.00
2110
PRIMER_RIGHT_17_SELF_END=2.00
2111
PRIMER_LEFT_17_END_STABILITY=9.7000
2112
PRIMER_RIGHT_17_END_STABILITY=7.9000
2113
PRIMER_PAIR_17_COMPL_ANY=4.00
2114
PRIMER_PAIR_17_COMPL_END=2.00
2115
PRIMER_PRODUCT_SIZE_17=158
2116
PRIMER_PAIR_PENALTY_18=1.3484
2117
PRIMER_LEFT_18_PENALTY=0.151542
2118
PRIMER_RIGHT_18_PENALTY=1.196903
2119
PRIMER_LEFT_18_SEQUENCE=TGGGTACAAACTGACAGGCA
2120
PRIMER_RIGHT_18_SEQUENCE=AACATGTGCACGCATGTAGAG
2121
PRIMER_LEFT_18=76,20
2122
PRIMER_RIGHT_18=242,21
2123
PRIMER_LEFT_18_TM=60.152
2124
PRIMER_RIGHT_18_TM=59.803
2125
PRIMER_LEFT_18_GC_PERCENT=50.000
2126
PRIMER_RIGHT_18_GC_PERCENT=47.619
2127
PRIMER_LEFT_18_SELF_ANY=5.00
2128
PRIMER_RIGHT_18_SELF_ANY=8.00
2129
PRIMER_LEFT_18_SELF_END=3.00
2130
PRIMER_RIGHT_18_SELF_END=2.00
2131
PRIMER_LEFT_18_END_STABILITY=9.7000
2132
PRIMER_RIGHT_18_END_STABILITY=5.7000
2133
PRIMER_PAIR_18_COMPL_ANY=4.00
2134
PRIMER_PAIR_18_COMPL_END=2.00
2135
PRIMER_PRODUCT_SIZE_18=167
2136
PRIMER_PAIR_PENALTY_19=1.3558
2137
PRIMER_LEFT_19_PENALTY=1.022788
2138
PRIMER_RIGHT_19_PENALTY=0.333053
2139
PRIMER_LEFT_19_SEQUENCE=TTTGGGTACAAACTGACAGGC
2140
PRIMER_RIGHT_19_SEQUENCE=TGTGCACGCATGTAGAGGTT
2141
PRIMER_LEFT_19=74,21
2142
PRIMER_RIGHT_19=238,20
2143
PRIMER_LEFT_19_TM=60.023
2144
PRIMER_RIGHT_19_TM=60.333
2145
PRIMER_LEFT_19_GC_PERCENT=47.619
2146
PRIMER_RIGHT_19_GC_PERCENT=50.000
2147
PRIMER_LEFT_19_SELF_ANY=5.00
2148
PRIMER_RIGHT_19_SELF_ANY=6.00
2149
PRIMER_LEFT_19_SELF_END=2.00
2150
PRIMER_RIGHT_19_SELF_END=0.00
2151
PRIMER_LEFT_19_END_STABILITY=9.7000
2152
PRIMER_RIGHT_19_END_STABILITY=7.9000
2153
PRIMER_PAIR_19_COMPL_ANY=4.00
2154
PRIMER_PAIR_19_COMPL_END=4.00
2155
PRIMER_PRODUCT_SIZE_19=165
2156
PRIMER_PAIR_PENALTY_20=1.3606
2157
PRIMER_LEFT_20_PENALTY=0.683672
2158
PRIMER_RIGHT_20_PENALTY=0.676947
2159
PRIMER_LEFT_20_SEQUENCE=GACAGGCAATTTCAAGGCTT
2160
PRIMER_RIGHT_20_SEQUENCE=GTGCACGCATGTAGAGGTTC
2161
PRIMER_LEFT_20=88,20
2162
PRIMER_RIGHT_20=237,20
2163
PRIMER_LEFT_20_TM=59.316
2164
PRIMER_RIGHT_20_TM=59.323
2165
PRIMER_LEFT_20_GC_PERCENT=45.000
2166
PRIMER_RIGHT_20_GC_PERCENT=55.000
2167
PRIMER_LEFT_20_SELF_ANY=5.00
2168
PRIMER_RIGHT_20_SELF_ANY=6.00
2169
PRIMER_LEFT_20_SELF_END=5.00
2170
PRIMER_RIGHT_20_SELF_END=1.00
2171
PRIMER_LEFT_20_END_STABILITY=9.7000
2172
PRIMER_RIGHT_20_END_STABILITY=7.9000
2173
PRIMER_PAIR_20_COMPL_ANY=4.00
2174
PRIMER_PAIR_20_COMPL_END=0.00
2175
PRIMER_PRODUCT_SIZE_20=150
2176
PRIMER_PAIR_PENALTY_21=1.3699
2177
PRIMER_LEFT_21_PENALTY=0.683672
2178
PRIMER_RIGHT_21_PENALTY=0.686256
2179
PRIMER_LEFT_21_SEQUENCE=GACAGGCAATTTCAAGGCTT
2180
PRIMER_RIGHT_21_SEQUENCE=CACGCATGTAGAGGTTCAGG
2181
PRIMER_LEFT_21=88,20
2182
PRIMER_RIGHT_21=234,20
2183
PRIMER_LEFT_21_TM=59.316
2184
PRIMER_RIGHT_21_TM=59.314
2185
PRIMER_LEFT_21_GC_PERCENT=45.000
2186
PRIMER_RIGHT_21_GC_PERCENT=55.000
2187
PRIMER_LEFT_21_SELF_ANY=5.00
2188
PRIMER_RIGHT_21_SELF_ANY=4.00
2189
PRIMER_LEFT_21_SELF_END=5.00
2190
PRIMER_RIGHT_21_SELF_END=0.00
2191
PRIMER_LEFT_21_END_STABILITY=9.7000
2192
PRIMER_RIGHT_21_END_STABILITY=8.2000
2193
PRIMER_PAIR_21_COMPL_ANY=4.00
2194
PRIMER_PAIR_21_COMPL_END=1.00
2195
PRIMER_PRODUCT_SIZE_21=147
2196
PRIMER_PAIR_PENALTY_22=1.3699
2197
PRIMER_LEFT_22_PENALTY=0.683672
2198
PRIMER_RIGHT_22_PENALTY=0.686256
2199
PRIMER_LEFT_22_SEQUENCE=GACAGGCAATTTCAAGGCTT
2200
PRIMER_RIGHT_22_SEQUENCE=CGCATGTAGAGGTTCAGGTG
2201
PRIMER_LEFT_22=88,20
2202
PRIMER_RIGHT_22=232,20
2203
PRIMER_LEFT_22_TM=59.316
2204
PRIMER_RIGHT_22_TM=59.314
2205
PRIMER_LEFT_22_GC_PERCENT=45.000
2206
PRIMER_RIGHT_22_GC_PERCENT=55.000
2207
PRIMER_LEFT_22_SELF_ANY=5.00
2208
PRIMER_RIGHT_22_SELF_ANY=4.00
2209
PRIMER_LEFT_22_SELF_END=5.00
2210
PRIMER_RIGHT_22_SELF_END=2.00
2211
PRIMER_LEFT_22_END_STABILITY=9.7000
2212
PRIMER_RIGHT_22_END_STABILITY=7.9000
2213
PRIMER_PAIR_22_COMPL_ANY=4.00
2214
PRIMER_PAIR_22_COMPL_END=0.00
2215
PRIMER_PRODUCT_SIZE_22=145
2216
PRIMER_PAIR_PENALTY_23=1.4139
2217
PRIMER_LEFT_23_PENALTY=0.775718
2218
PRIMER_RIGHT_23_PENALTY=0.638191
2219
PRIMER_LEFT_23_SEQUENCE=TGACAGGCAATTTCAAGGCT
2220
PRIMER_RIGHT_23_SEQUENCE=AGAGGTTCAGGTGGAGAGGG
2221
PRIMER_LEFT_23=87,20
2222
PRIMER_RIGHT_23=225,20
2223
PRIMER_LEFT_23_TM=60.776
2224
PRIMER_RIGHT_23_TM=60.638
2225
PRIMER_LEFT_23_GC_PERCENT=45.000
2226
PRIMER_RIGHT_23_GC_PERCENT=60.000
2227
PRIMER_LEFT_23_SELF_ANY=4.00
2228
PRIMER_RIGHT_23_SELF_ANY=2.00
2229
PRIMER_LEFT_23_SELF_END=3.00
2230
PRIMER_RIGHT_23_SELF_END=0.00
2231
PRIMER_LEFT_23_END_STABILITY=9.4000
2232
PRIMER_RIGHT_23_END_STABILITY=9.4000
2233
PRIMER_PAIR_23_COMPL_ANY=3.00
2234
PRIMER_PAIR_23_COMPL_END=0.00
2235
PRIMER_PRODUCT_SIZE_23=139
2236
PRIMER_PAIR_PENALTY_24=1.4139
2237
PRIMER_LEFT_24_PENALTY=0.775718
2238
PRIMER_RIGHT_24_PENALTY=0.638191
2239
PRIMER_LEFT_24_SEQUENCE=CTGACAGGCAATTTCAAGGC
2240
PRIMER_RIGHT_24_SEQUENCE=AGAGGTTCAGGTGGAGAGGG
2241
PRIMER_LEFT_24=86,20
2242
PRIMER_RIGHT_24=225,20
2243
PRIMER_LEFT_24_TM=60.776
2244
PRIMER_RIGHT_24_TM=60.638
2245
PRIMER_LEFT_24_GC_PERCENT=50.000
2246
PRIMER_RIGHT_24_GC_PERCENT=60.000
2247
PRIMER_LEFT_24_SELF_ANY=5.00
2248
PRIMER_RIGHT_24_SELF_ANY=2.00
2249
PRIMER_LEFT_24_SELF_END=2.00
2250
PRIMER_RIGHT_24_SELF_END=0.00
2251
PRIMER_LEFT_24_END_STABILITY=9.7000
2252
PRIMER_RIGHT_24_END_STABILITY=9.4000
2253
PRIMER_PAIR_24_COMPL_ANY=4.00
2254
PRIMER_PAIR_24_COMPL_END=1.00
2255
PRIMER_PRODUCT_SIZE_24=140
2256
PRIMER_PAIR_PENALTY_25=1.4178
2257
PRIMER_LEFT_25_PENALTY=0.151542
2258
PRIMER_RIGHT_25_PENALTY=1.266285
2259
PRIMER_LEFT_25_SEQUENCE=TGGGTACAAACTGACAGGCA
2260
PRIMER_RIGHT_25_SEQUENCE=AACATGTGCACGCATGTAGA
2261
PRIMER_LEFT_25=76,20
2262
PRIMER_RIGHT_25=242,20
2263
PRIMER_LEFT_25_TM=60.152
2264
PRIMER_RIGHT_25_TM=58.734
2265
PRIMER_LEFT_25_GC_PERCENT=50.000
2266
PRIMER_RIGHT_25_GC_PERCENT=45.000
2267
PRIMER_LEFT_25_SELF_ANY=5.00
2268
PRIMER_RIGHT_25_SELF_ANY=8.00
2269
PRIMER_LEFT_25_SELF_END=3.00
2270
PRIMER_RIGHT_25_SELF_END=4.00
2271
PRIMER_LEFT_25_END_STABILITY=9.7000
2272
PRIMER_RIGHT_25_END_STABILITY=5.4000
2273
PRIMER_PAIR_25_COMPL_ANY=4.00
2274
PRIMER_PAIR_25_COMPL_END=2.00
2275
PRIMER_PRODUCT_SIZE_25=167
2276
PRIMER_PAIR_PENALTY_26=1.4178
2277
PRIMER_LEFT_26_PENALTY=0.151542
2278
PRIMER_RIGHT_26_PENALTY=1.266285
2279
PRIMER_LEFT_26_SEQUENCE=TGGGTACAAACTGACAGGCA
2280
PRIMER_RIGHT_26_SEQUENCE=GAACATGTGCACGCATGTAG
2281
PRIMER_LEFT_26=76,20
2282
PRIMER_RIGHT_26=243,20
2283
PRIMER_LEFT_26_TM=60.152
2284
PRIMER_RIGHT_26_TM=58.734
2285
PRIMER_LEFT_26_GC_PERCENT=50.000
2286
PRIMER_RIGHT_26_GC_PERCENT=50.000
2287
PRIMER_LEFT_26_SELF_ANY=5.00
2288
PRIMER_RIGHT_26_SELF_ANY=8.00
2289
PRIMER_LEFT_26_SELF_END=3.00
2290
PRIMER_RIGHT_26_SELF_END=4.00
2291
PRIMER_LEFT_26_END_STABILITY=9.7000
2292
PRIMER_RIGHT_26_END_STABILITY=5.7000
2293
PRIMER_PAIR_26_COMPL_ANY=4.00
2294
PRIMER_PAIR_26_COMPL_END=3.00
2295
PRIMER_PRODUCT_SIZE_26=168
2296
PRIMER_PAIR_PENALTY_27=1.4502
2297
PRIMER_LEFT_27_PENALTY=0.683672
2298
PRIMER_RIGHT_27_PENALTY=0.766499
2299
PRIMER_LEFT_27_SEQUENCE=GACAGGCAATTTCAAGGCTT
2300
PRIMER_RIGHT_27_SEQUENCE=TGAACATGTGCACGCATGTA
2301
PRIMER_LEFT_27=88,20
2302
PRIMER_RIGHT_27=244,20
2303
PRIMER_LEFT_27_TM=59.316
2304
PRIMER_RIGHT_27_TM=60.766
2305
PRIMER_LEFT_27_GC_PERCENT=45.000
2306
PRIMER_RIGHT_27_GC_PERCENT=45.000
2307
PRIMER_LEFT_27_SELF_ANY=5.00
2308
PRIMER_RIGHT_27_SELF_ANY=8.00
2309
PRIMER_LEFT_27_SELF_END=5.00
2310
PRIMER_RIGHT_27_SELF_END=6.00
2311
PRIMER_LEFT_27_END_STABILITY=9.7000
2312
PRIMER_RIGHT_27_END_STABILITY=5.6000
2313
PRIMER_PAIR_27_COMPL_ANY=5.00
2314
PRIMER_PAIR_27_COMPL_END=1.00
2315
PRIMER_PRODUCT_SIZE_27=157
2316
PRIMER_PAIR_PENALTY_28=1.4527
2317
PRIMER_LEFT_28_PENALTY=0.775718
2318
PRIMER_RIGHT_28_PENALTY=0.676947
2319
PRIMER_LEFT_28_SEQUENCE=CTGACAGGCAATTTCAAGGC
2320
PRIMER_RIGHT_28_SEQUENCE=GTGCACGCATGTAGAGGTTC
2321
PRIMER_LEFT_28=86,20
2322
PRIMER_RIGHT_28=237,20
2323
PRIMER_LEFT_28_TM=60.776
2324
PRIMER_RIGHT_28_TM=59.323
2325
PRIMER_LEFT_28_GC_PERCENT=50.000
2326
PRIMER_RIGHT_28_GC_PERCENT=55.000
2327
PRIMER_LEFT_28_SELF_ANY=5.00
2328
PRIMER_RIGHT_28_SELF_ANY=6.00
2329
PRIMER_LEFT_28_SELF_END=2.00
2330
PRIMER_RIGHT_28_SELF_END=1.00
2331
PRIMER_LEFT_28_END_STABILITY=9.7000
2332
PRIMER_RIGHT_28_END_STABILITY=7.9000
2333
PRIMER_PAIR_28_COMPL_ANY=4.00
2334
PRIMER_PAIR_28_COMPL_END=0.00
2335
PRIMER_PRODUCT_SIZE_28=152
2336
PRIMER_PAIR_PENALTY_29=1.4527
2337
PRIMER_LEFT_29_PENALTY=0.775718
2338
PRIMER_RIGHT_29_PENALTY=0.676947
2339
PRIMER_LEFT_29_SEQUENCE=TGACAGGCAATTTCAAGGCT
2340
PRIMER_RIGHT_29_SEQUENCE=GTGCACGCATGTAGAGGTTC
2341
PRIMER_LEFT_29=87,20
2342
PRIMER_RIGHT_29=237,20
2343
PRIMER_LEFT_29_TM=60.776
2344
PRIMER_RIGHT_29_TM=59.323
2345
PRIMER_LEFT_29_GC_PERCENT=45.000
2346
PRIMER_RIGHT_29_GC_PERCENT=55.000
2347
PRIMER_LEFT_29_SELF_ANY=4.00
2348
PRIMER_RIGHT_29_SELF_ANY=6.00
2349
PRIMER_LEFT_29_SELF_END=3.00
2350
PRIMER_RIGHT_29_SELF_END=1.00
2351
PRIMER_LEFT_29_END_STABILITY=9.4000
2352
PRIMER_RIGHT_29_END_STABILITY=7.9000
2353
PRIMER_PAIR_29_COMPL_ANY=4.00
2354
PRIMER_PAIR_29_COMPL_END=0.00
2355
PRIMER_PRODUCT_SIZE_29=151
2356
PRIMER_PAIR_PENALTY_30=1.4620
2357
PRIMER_LEFT_30_PENALTY=0.775718
2358
PRIMER_RIGHT_30_PENALTY=0.686256
2359
PRIMER_LEFT_30_SEQUENCE=CTGACAGGCAATTTCAAGGC
2360
PRIMER_RIGHT_30_SEQUENCE=CACGCATGTAGAGGTTCAGG
2361
PRIMER_LEFT_30=86,20
2362
PRIMER_RIGHT_30=234,20
2363
PRIMER_LEFT_30_TM=60.776
2364
PRIMER_RIGHT_30_TM=59.314
2365
PRIMER_LEFT_30_GC_PERCENT=50.000
2366
PRIMER_RIGHT_30_GC_PERCENT=55.000
2367
PRIMER_LEFT_30_SELF_ANY=5.00
2368
PRIMER_RIGHT_30_SELF_ANY=4.00
2369
PRIMER_LEFT_30_SELF_END=2.00
2370
PRIMER_RIGHT_30_SELF_END=0.00
2371
PRIMER_LEFT_30_END_STABILITY=9.7000
2372
PRIMER_RIGHT_30_END_STABILITY=8.2000
2373
PRIMER_PAIR_30_COMPL_ANY=4.00
2374
PRIMER_PAIR_30_COMPL_END=1.00
2375
PRIMER_PRODUCT_SIZE_30=149
2376
PRIMER_PAIR_PENALTY_31=1.4620
2377
PRIMER_LEFT_31_PENALTY=0.775718
2378
PRIMER_RIGHT_31_PENALTY=0.686256
2379
PRIMER_LEFT_31_SEQUENCE=TGACAGGCAATTTCAAGGCT
2380
PRIMER_RIGHT_31_SEQUENCE=CACGCATGTAGAGGTTCAGG
2381
PRIMER_LEFT_31=87,20
2382
PRIMER_RIGHT_31=234,20
2383
PRIMER_LEFT_31_TM=60.776
2384
PRIMER_RIGHT_31_TM=59.314
2385
PRIMER_LEFT_31_GC_PERCENT=45.000
2386
PRIMER_RIGHT_31_GC_PERCENT=55.000
2387
PRIMER_LEFT_31_SELF_ANY=4.00
2388
PRIMER_RIGHT_31_SELF_ANY=4.00
2389
PRIMER_LEFT_31_SELF_END=3.00
2390
PRIMER_RIGHT_31_SELF_END=0.00
2391
PRIMER_LEFT_31_END_STABILITY=9.4000
2392
PRIMER_RIGHT_31_END_STABILITY=8.2000
2393
PRIMER_PAIR_31_COMPL_ANY=4.00
2394
PRIMER_PAIR_31_COMPL_END=1.00
2395
PRIMER_PRODUCT_SIZE_31=148
2396
PRIMER_PAIR_PENALTY_32=1.4620
2397
PRIMER_LEFT_32_PENALTY=0.775718
2398
PRIMER_RIGHT_32_PENALTY=0.686256
2399
PRIMER_LEFT_32_SEQUENCE=TGACAGGCAATTTCAAGGCT
2400
PRIMER_RIGHT_32_SEQUENCE=CGCATGTAGAGGTTCAGGTG
2401
PRIMER_LEFT_32=87,20
2402
PRIMER_RIGHT_32=232,20
2403
PRIMER_LEFT_32_TM=60.776
2404
PRIMER_RIGHT_32_TM=59.314
2405
PRIMER_LEFT_32_GC_PERCENT=45.000
2406
PRIMER_RIGHT_32_GC_PERCENT=55.000
2407
PRIMER_LEFT_32_SELF_ANY=4.00
2408
PRIMER_RIGHT_32_SELF_ANY=4.00
2409
PRIMER_LEFT_32_SELF_END=3.00
2410
PRIMER_RIGHT_32_SELF_END=2.00
2411
PRIMER_LEFT_32_END_STABILITY=9.4000
2412
PRIMER_RIGHT_32_END_STABILITY=7.9000
2413
PRIMER_PAIR_32_COMPL_ANY=4.00
2414
PRIMER_PAIR_32_COMPL_END=0.00
2415
PRIMER_PRODUCT_SIZE_32=146
2416
PRIMER_PAIR_PENALTY_33=1.4620
2417
PRIMER_LEFT_33_PENALTY=0.775718
2418
PRIMER_RIGHT_33_PENALTY=0.686256
2419
PRIMER_LEFT_33_SEQUENCE=CTGACAGGCAATTTCAAGGC
2420
PRIMER_RIGHT_33_SEQUENCE=CGCATGTAGAGGTTCAGGTG
2421
PRIMER_LEFT_33=86,20
2422
PRIMER_RIGHT_33=232,20
2423
PRIMER_LEFT_33_TM=60.776
2424
PRIMER_RIGHT_33_TM=59.314
2425
PRIMER_LEFT_33_GC_PERCENT=50.000
2426
PRIMER_RIGHT_33_GC_PERCENT=55.000
2427
PRIMER_LEFT_33_SELF_ANY=5.00
2428
PRIMER_RIGHT_33_SELF_ANY=4.00
2429
PRIMER_LEFT_33_SELF_END=2.00
2430
PRIMER_RIGHT_33_SELF_END=2.00
2431
PRIMER_LEFT_33_END_STABILITY=9.7000
2432
PRIMER_RIGHT_33_END_STABILITY=7.9000
2433
PRIMER_PAIR_33_COMPL_ANY=4.00
2434
PRIMER_PAIR_33_COMPL_END=1.00
2435
PRIMER_PRODUCT_SIZE_33=147
2436
PRIMER_PAIR_PENALTY_34=1.4697
2437
PRIMER_LEFT_34_PENALTY=0.151542
2438
PRIMER_RIGHT_34_PENALTY=1.318201
2439
PRIMER_LEFT_34_SEQUENCE=TGGGTACAAACTGACAGGCA
2440
PRIMER_RIGHT_34_SEQUENCE=CATGTGCACGCATGTAGAGG
2441
PRIMER_LEFT_34=76,20
2442
PRIMER_RIGHT_34=240,20
2443
PRIMER_LEFT_34_TM=60.152
2444
PRIMER_RIGHT_34_TM=61.318
2445
PRIMER_LEFT_34_GC_PERCENT=50.000
2446
PRIMER_RIGHT_34_GC_PERCENT=55.000
2447
PRIMER_LEFT_34_SELF_ANY=5.00
2448
PRIMER_RIGHT_34_SELF_ANY=6.00
2449
PRIMER_LEFT_34_SELF_END=3.00
2450
PRIMER_RIGHT_34_SELF_END=4.00
2451
PRIMER_LEFT_34_END_STABILITY=9.7000
2452
PRIMER_RIGHT_34_END_STABILITY=7.9000
2453
PRIMER_PAIR_34_COMPL_ANY=4.00
2454
PRIMER_PAIR_34_COMPL_END=1.00
2455
PRIMER_PRODUCT_SIZE_34=165
2456
PRIMER_PAIR_PENALTY_35=1.4884
2457
PRIMER_LEFT_35_PENALTY=0.683672
2458
PRIMER_RIGHT_35_PENALTY=0.804687
2459
PRIMER_LEFT_35_SEQUENCE=GACAGGCAATTTCAAGGCTT
2460
PRIMER_RIGHT_35_SEQUENCE=ATGTGCACGCATGTAGAGGT
2461
PRIMER_LEFT_35=88,20
2462
PRIMER_RIGHT_35=239,20
2463
PRIMER_LEFT_35_TM=59.316
2464
PRIMER_RIGHT_35_TM=59.195
2465
PRIMER_LEFT_35_GC_PERCENT=45.000
2466
PRIMER_RIGHT_35_GC_PERCENT=50.000
2467
PRIMER_LEFT_35_SELF_ANY=5.00
2468
PRIMER_RIGHT_35_SELF_ANY=6.00
2469
PRIMER_LEFT_35_SELF_END=5.00
2470
PRIMER_RIGHT_35_SELF_END=0.00
2471
PRIMER_LEFT_35_END_STABILITY=9.7000
2472
PRIMER_RIGHT_35_END_STABILITY=7.6000
2473
PRIMER_PAIR_35_COMPL_ANY=4.00
2474
PRIMER_PAIR_35_COMPL_END=0.00
2475
PRIMER_PRODUCT_SIZE_35=152
2476
PRIMER_PAIR_PENALTY_36=1.5294
2477
PRIMER_LEFT_36_PENALTY=0.151542
2478
PRIMER_RIGHT_36_PENALTY=1.377815
2479
PRIMER_LEFT_36_SEQUENCE=TGGGTACAAACTGACAGGCA
2480
PRIMER_RIGHT_36_SEQUENCE=GAGGTTCAGGTGGAGAGGG
2481
PRIMER_LEFT_36=76,20
2482
PRIMER_RIGHT_36=224,19
2483
PRIMER_LEFT_36_TM=60.152
2484
PRIMER_RIGHT_36_TM=59.622
2485
PRIMER_LEFT_36_GC_PERCENT=50.000
2486
PRIMER_RIGHT_36_GC_PERCENT=63.158
2487
PRIMER_LEFT_36_SELF_ANY=5.00
2488
PRIMER_RIGHT_36_SELF_ANY=2.00
2489
PRIMER_LEFT_36_SELF_END=3.00
2490
PRIMER_RIGHT_36_SELF_END=0.00
2491
PRIMER_LEFT_36_END_STABILITY=9.7000
2492
PRIMER_RIGHT_36_END_STABILITY=9.4000
2493
PRIMER_PAIR_36_COMPL_ANY=4.00
2494
PRIMER_PAIR_36_COMPL_END=0.00
2495
PRIMER_PRODUCT_SIZE_36=149
2496
PRIMER_PAIR_PENALTY_37=1.5422
2497
PRIMER_LEFT_37_PENALTY=0.775718
2498
PRIMER_RIGHT_37_PENALTY=0.766499
2499
PRIMER_LEFT_37_SEQUENCE=CTGACAGGCAATTTCAAGGC
2500
PRIMER_RIGHT_37_SEQUENCE=TGAACATGTGCACGCATGTA
2501
PRIMER_LEFT_37=86,20
2502
PRIMER_RIGHT_37=244,20
2503
PRIMER_LEFT_37_TM=60.776
2504
PRIMER_RIGHT_37_TM=60.766
2505
PRIMER_LEFT_37_GC_PERCENT=50.000
2506
PRIMER_RIGHT_37_GC_PERCENT=45.000
2507
PRIMER_LEFT_37_SELF_ANY=5.00
2508
PRIMER_RIGHT_37_SELF_ANY=8.00
2509
PRIMER_LEFT_37_SELF_END=2.00
2510
PRIMER_RIGHT_37_SELF_END=6.00
2511
PRIMER_LEFT_37_END_STABILITY=9.7000
2512
PRIMER_RIGHT_37_END_STABILITY=5.6000
2513
PRIMER_PAIR_37_COMPL_ANY=5.00
2514
PRIMER_PAIR_37_COMPL_END=1.00
2515
PRIMER_PRODUCT_SIZE_37=159
2516
PRIMER_PAIR_PENALTY_38=1.5422
2517
PRIMER_LEFT_38_PENALTY=0.775718
2518
PRIMER_RIGHT_38_PENALTY=0.766499
2519
PRIMER_LEFT_38_SEQUENCE=TGACAGGCAATTTCAAGGCT
2520
PRIMER_RIGHT_38_SEQUENCE=TGAACATGTGCACGCATGTA
2521
PRIMER_LEFT_38=87,20
2522
PRIMER_RIGHT_38=244,20
2523
PRIMER_LEFT_38_TM=60.776
2524
PRIMER_RIGHT_38_TM=60.766
2525
PRIMER_LEFT_38_GC_PERCENT=45.000
2526
PRIMER_RIGHT_38_GC_PERCENT=45.000
2527
PRIMER_LEFT_38_SELF_ANY=4.00
2528
PRIMER_RIGHT_38_SELF_ANY=8.00
2529
PRIMER_LEFT_38_SELF_END=3.00
2530
PRIMER_RIGHT_38_SELF_END=6.00
2531
PRIMER_LEFT_38_END_STABILITY=9.4000
2532
PRIMER_RIGHT_38_END_STABILITY=5.6000
2533
PRIMER_PAIR_38_COMPL_ANY=5.00
2534
PRIMER_PAIR_38_COMPL_END=1.00
2535
PRIMER_PRODUCT_SIZE_38=158
2536
PRIMER_PAIR_PENALTY_39=1.5431
2537
PRIMER_LEFT_39_PENALTY=1.210088
2538
PRIMER_RIGHT_39_PENALTY=0.333053
2539
PRIMER_LEFT_39_SEQUENCE=TGACAGGCAATTTCAAGGC
2540
PRIMER_RIGHT_39_SEQUENCE=TGTGCACGCATGTAGAGGTT
2541
PRIMER_LEFT_39=87,19
2542
PRIMER_RIGHT_39=238,20
2543
PRIMER_LEFT_39_TM=59.790
2544
PRIMER_RIGHT_39_TM=60.333
2545
PRIMER_LEFT_39_GC_PERCENT=47.368
2546
PRIMER_RIGHT_39_GC_PERCENT=50.000
2547
PRIMER_LEFT_39_SELF_ANY=4.00
2548
PRIMER_RIGHT_39_SELF_ANY=6.00
2549
PRIMER_LEFT_39_SELF_END=2.00
2550
PRIMER_RIGHT_39_SELF_END=0.00
2551
PRIMER_LEFT_39_END_STABILITY=9.7000
2552
PRIMER_RIGHT_39_END_STABILITY=7.9000
2553
PRIMER_PAIR_39_COMPL_ANY=4.00
2554
PRIMER_PAIR_39_COMPL_END=1.00
2555
PRIMER_PRODUCT_SIZE_39=152
2556
=
2557
MARKER_NAME=08.RHAP34FE8.seq
2558
SEQUENCE=NNNNNNNNNNNNNNNNNNNNCATGCAGTGTGAATATCTCATGTTCTTCAGCTTTTTCCCCTTAGGACCAAGGTATAGCCTTAGGACCACGGATTATCCTTCCATTTTCTTAGAACTCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTGAGTGGCATCTGTCCCACCAGGTCCTTTGAAGAGCTGAGTGAAATGATGTATAAAGTACTTTGCATTGGCAAGGGGGAAGACTCCAGCATGGATCTAGTAGAAGAAATATGAAGGAG
2559
INCLUDED_REGION=20,281
2560
TARGET=119,62,AC
2561
PRIMER_PAIR_PENALTY=0.2190
2562
PRIMER_LEFT_PENALTY=0.201280
2563
PRIMER_RIGHT_PENALTY=0.017711
2564
PRIMER_LEFT_SEQUENCE=TTTCCCCTTAGGACCAAGGT
2565
PRIMER_RIGHT_SEQUENCE=CAGCTCTTCAAAGGACCTGG
2566
PRIMER_LEFT=53,20
2567
PRIMER_RIGHT=220,20
2568
PRIMER_LEFT_TM=59.799
2569
PRIMER_RIGHT_TM=59.982
2570
PRIMER_LEFT_GC_PERCENT=50.000
2571
PRIMER_RIGHT_GC_PERCENT=55.000
2572
PRIMER_LEFT_SELF_ANY=7.00
2573
PRIMER_RIGHT_SELF_ANY=6.00
2574
PRIMER_LEFT_SELF_END=5.00
2575
PRIMER_RIGHT_SELF_END=4.00
2576
PRIMER_LEFT_END_STABILITY=7.9000
2577
PRIMER_RIGHT_END_STABILITY=9.7000
2578
PRIMER_PAIR_COMPL_ANY=4.00
2579
PRIMER_PAIR_COMPL_END=4.00
2580
PRIMER_PRODUCT_SIZE=168
2581
PRIMER_PAIR_PENALTY_1=0.2677
2582
PRIMER_LEFT_1_PENALTY=0.249989
2583
PRIMER_RIGHT_1_PENALTY=0.017711
2584
PRIMER_LEFT_1_SEQUENCE=GACCACGGATTATCCTTCCA
2585
PRIMER_RIGHT_1_SEQUENCE=CAGCTCTTCAAAGGACCTGG
2586
PRIMER_LEFT_1=83,20
2587
PRIMER_RIGHT_1=220,20
2588
PRIMER_LEFT_1_TM=59.750
2589
PRIMER_RIGHT_1_TM=59.982
2590
PRIMER_LEFT_1_GC_PERCENT=50.000
2591
PRIMER_RIGHT_1_GC_PERCENT=55.000
2592
PRIMER_LEFT_1_SELF_ANY=7.00
2593
PRIMER_RIGHT_1_SELF_ANY=6.00
2594
PRIMER_LEFT_1_SELF_END=1.00
2595
PRIMER_RIGHT_1_SELF_END=4.00
2596
PRIMER_LEFT_1_END_STABILITY=8.5000
2597
PRIMER_RIGHT_1_END_STABILITY=9.7000
2598
PRIMER_PAIR_1_COMPL_ANY=5.00
2599
PRIMER_PAIR_1_COMPL_END=3.00
2600
PRIMER_PRODUCT_SIZE_1=138
2601
PRIMER_PAIR_PENALTY_2=0.3573
2602
PRIMER_LEFT_2_PENALTY=0.201280
2603
PRIMER_RIGHT_2_PENALTY=0.156061
2604
PRIMER_LEFT_2_SEQUENCE=TTTCCCCTTAGGACCAAGGT
2605
PRIMER_RIGHT_2_SEQUENCE=GCTCTTCAAAGGACCTGGTG
2606
PRIMER_LEFT_2=53,20
2607
PRIMER_RIGHT_2=218,20
2608
PRIMER_LEFT_2_TM=59.799
2609
PRIMER_RIGHT_2_TM=59.844
2610
PRIMER_LEFT_2_GC_PERCENT=50.000
2611
PRIMER_RIGHT_2_GC_PERCENT=55.000
2612
PRIMER_LEFT_2_SELF_ANY=7.00
2613
PRIMER_RIGHT_2_SELF_ANY=5.00
2614
PRIMER_LEFT_2_SELF_END=5.00
2615
PRIMER_RIGHT_2_SELF_END=3.00
2616
PRIMER_LEFT_2_END_STABILITY=7.9000
2617
PRIMER_RIGHT_2_END_STABILITY=8.2000
2618
PRIMER_PAIR_2_COMPL_ANY=5.00
2619
PRIMER_PAIR_2_COMPL_END=4.00
2620
PRIMER_PRODUCT_SIZE_2=166
2621
PRIMER_PAIR_PENALTY_3=0.4060
2622
PRIMER_LEFT_3_PENALTY=0.249989
2623
PRIMER_RIGHT_3_PENALTY=0.156061
2624
PRIMER_LEFT_3_SEQUENCE=GACCACGGATTATCCTTCCA
2625
PRIMER_RIGHT_3_SEQUENCE=GCTCTTCAAAGGACCTGGTG
2626
PRIMER_LEFT_3=83,20
2627
PRIMER_RIGHT_3=218,20
2628
PRIMER_LEFT_3_TM=59.750
2629
PRIMER_RIGHT_3_TM=59.844
2630
PRIMER_LEFT_3_GC_PERCENT=50.000
2631
PRIMER_RIGHT_3_GC_PERCENT=55.000
2632
PRIMER_LEFT_3_SELF_ANY=7.00
2633
PRIMER_RIGHT_3_SELF_ANY=5.00
2634
PRIMER_LEFT_3_SELF_END=1.00
2635
PRIMER_RIGHT_3_SELF_END=3.00
2636
PRIMER_LEFT_3_END_STABILITY=8.5000
2637
PRIMER_RIGHT_3_END_STABILITY=8.2000
2638
PRIMER_PAIR_3_COMPL_ANY=5.00
2639
PRIMER_PAIR_3_COMPL_END=2.00
2640
PRIMER_PRODUCT_SIZE_3=136
2641
PRIMER_PAIR_PENALTY_4=0.4406
2642
PRIMER_LEFT_4_PENALTY=0.422888
2643
PRIMER_RIGHT_4_PENALTY=0.017711
2644
PRIMER_LEFT_4_SEQUENCE=GCTTTTTCCCCTTAGGACCA
2645
PRIMER_RIGHT_4_SEQUENCE=CAGCTCTTCAAAGGACCTGG
2646
PRIMER_LEFT_4=49,20
2647
PRIMER_RIGHT_4=220,20
2648
PRIMER_LEFT_4_TM=60.423
2649
PRIMER_RIGHT_4_TM=59.982
2650
PRIMER_LEFT_4_GC_PERCENT=50.000
2651
PRIMER_RIGHT_4_GC_PERCENT=55.000
2652
PRIMER_LEFT_4_SELF_ANY=5.00
2653
PRIMER_RIGHT_4_SELF_ANY=6.00
2654
PRIMER_LEFT_4_SELF_END=1.00
2655
PRIMER_RIGHT_4_SELF_END=4.00
2656
PRIMER_LEFT_4_END_STABILITY=7.9000
2657
PRIMER_RIGHT_4_END_STABILITY=9.7000
2658
PRIMER_PAIR_4_COMPL_ANY=4.00
2659
PRIMER_PAIR_4_COMPL_END=3.00
2660
PRIMER_PRODUCT_SIZE_4=172
2661
PRIMER_PAIR_PENALTY_5=0.5102
2662
PRIMER_LEFT_5_PENALTY=0.201280
2663
PRIMER_RIGHT_5_PENALTY=0.308871
2664
PRIMER_LEFT_5_SEQUENCE=TTTCCCCTTAGGACCAAGGT
2665
PRIMER_RIGHT_5_SEQUENCE=CTCTTCAAAGGACCTGGTGG
2666
PRIMER_LEFT_5=53,20
2667
PRIMER_RIGHT_5=217,20
2668
PRIMER_LEFT_5_TM=59.799
2669
PRIMER_RIGHT_5_TM=59.691
2670
PRIMER_LEFT_5_GC_PERCENT=50.000
2671
PRIMER_RIGHT_5_GC_PERCENT=55.000
2672
PRIMER_LEFT_5_SELF_ANY=7.00
2673
PRIMER_RIGHT_5_SELF_ANY=5.00
2674
PRIMER_LEFT_5_SELF_END=5.00
2675
PRIMER_RIGHT_5_SELF_END=1.00
2676
PRIMER_LEFT_5_END_STABILITY=7.9000
2677
PRIMER_RIGHT_5_END_STABILITY=9.4000
2678
PRIMER_PAIR_5_COMPL_ANY=5.00
2679
PRIMER_PAIR_5_COMPL_END=3.00
2680
PRIMER_PRODUCT_SIZE_5=165
2681
PRIMER_PAIR_PENALTY_6=0.5433
2682
PRIMER_LEFT_6_PENALTY=0.525579
2683
PRIMER_RIGHT_6_PENALTY=0.017711
2684
PRIMER_LEFT_6_SEQUENCE=GGACCACGGATTATCCTTCC
2685
PRIMER_RIGHT_6_SEQUENCE=CAGCTCTTCAAAGGACCTGG
2686
PRIMER_LEFT_6=82,20
2687
PRIMER_RIGHT_6=220,20
2688
PRIMER_LEFT_6_TM=60.526
2689
PRIMER_RIGHT_6_TM=59.982
2690
PRIMER_LEFT_6_GC_PERCENT=55.000
2691
PRIMER_RIGHT_6_GC_PERCENT=55.000
2692
PRIMER_LEFT_6_SELF_ANY=7.00
2693
PRIMER_RIGHT_6_SELF_ANY=6.00
2694
PRIMER_LEFT_6_SELF_END=3.00
2695
PRIMER_RIGHT_6_SELF_END=4.00
2696
PRIMER_LEFT_6_END_STABILITY=8.2000
2697
PRIMER_RIGHT_6_END_STABILITY=9.7000
2698
PRIMER_PAIR_6_COMPL_ANY=5.00
2699
PRIMER_PAIR_6_COMPL_END=2.00
2700
PRIMER_PRODUCT_SIZE_6=139
2701
PRIMER_PAIR_PENALTY_7=0.5589
2702
PRIMER_LEFT_7_PENALTY=0.249989
2703
PRIMER_RIGHT_7_PENALTY=0.308871
2704
PRIMER_LEFT_7_SEQUENCE=GACCACGGATTATCCTTCCA
2705
PRIMER_RIGHT_7_SEQUENCE=CTCTTCAAAGGACCTGGTGG
2706
PRIMER_LEFT_7=83,20
2707
PRIMER_RIGHT_7=217,20
2708
PRIMER_LEFT_7_TM=59.750
2709
PRIMER_RIGHT_7_TM=59.691
2710
PRIMER_LEFT_7_GC_PERCENT=50.000
2711
PRIMER_RIGHT_7_GC_PERCENT=55.000
2712
PRIMER_LEFT_7_SELF_ANY=7.00
2713
PRIMER_RIGHT_7_SELF_ANY=5.00
2714
PRIMER_LEFT_7_SELF_END=1.00
2715
PRIMER_RIGHT_7_SELF_END=1.00
2716
PRIMER_LEFT_7_END_STABILITY=8.5000
2717
PRIMER_RIGHT_7_END_STABILITY=9.4000
2718
PRIMER_PAIR_7_COMPL_ANY=5.00
2719
PRIMER_PAIR_7_COMPL_END=3.00
2720
PRIMER_PRODUCT_SIZE_7=135
2721
PRIMER_PAIR_PENALTY_8=0.5789
2722
PRIMER_LEFT_8_PENALTY=0.422888
2723
PRIMER_RIGHT_8_PENALTY=0.156061
2724
PRIMER_LEFT_8_SEQUENCE=GCTTTTTCCCCTTAGGACCA
2725
PRIMER_RIGHT_8_SEQUENCE=GCTCTTCAAAGGACCTGGTG
2726
PRIMER_LEFT_8=49,20
2727
PRIMER_RIGHT_8=218,20
2728
PRIMER_LEFT_8_TM=60.423
2729
PRIMER_RIGHT_8_TM=59.844
2730
PRIMER_LEFT_8_GC_PERCENT=50.000
2731
PRIMER_RIGHT_8_GC_PERCENT=55.000
2732
PRIMER_LEFT_8_SELF_ANY=5.00
2733
PRIMER_RIGHT_8_SELF_ANY=5.00
2734
PRIMER_LEFT_8_SELF_END=1.00
2735
PRIMER_RIGHT_8_SELF_END=3.00
2736
PRIMER_LEFT_8_END_STABILITY=7.9000
2737
PRIMER_RIGHT_8_END_STABILITY=8.2000
2738
PRIMER_PAIR_8_COMPL_ANY=4.00
2739
PRIMER_PAIR_8_COMPL_END=3.00
2740
PRIMER_PRODUCT_SIZE_8=170
2741
PRIMER_PAIR_PENALTY_9=0.6816
2742
PRIMER_LEFT_9_PENALTY=0.525579
2743
PRIMER_RIGHT_9_PENALTY=0.156061
2744
PRIMER_LEFT_9_SEQUENCE=GGACCACGGATTATCCTTCC
2745
PRIMER_RIGHT_9_SEQUENCE=GCTCTTCAAAGGACCTGGTG
2746
PRIMER_LEFT_9=82,20
2747
PRIMER_RIGHT_9=218,20
2748
PRIMER_LEFT_9_TM=60.526
2749
PRIMER_RIGHT_9_TM=59.844
2750
PRIMER_LEFT_9_GC_PERCENT=55.000
2751
PRIMER_RIGHT_9_GC_PERCENT=55.000
2752
PRIMER_LEFT_9_SELF_ANY=7.00
2753
PRIMER_RIGHT_9_SELF_ANY=5.00
2754
PRIMER_LEFT_9_SELF_END=3.00
2755
PRIMER_RIGHT_9_SELF_END=3.00
2756
PRIMER_LEFT_9_END_STABILITY=8.2000
2757
PRIMER_RIGHT_9_END_STABILITY=8.2000
2758
PRIMER_PAIR_9_COMPL_ANY=5.00
2759
PRIMER_PAIR_9_COMPL_END=1.00
2760
PRIMER_PRODUCT_SIZE_9=137
2761
PRIMER_PAIR_PENALTY_10=0.7318
2762
PRIMER_LEFT_10_PENALTY=0.422888
2763
PRIMER_RIGHT_10_PENALTY=0.308871
2764
PRIMER_LEFT_10_SEQUENCE=GCTTTTTCCCCTTAGGACCA
2765
PRIMER_RIGHT_10_SEQUENCE=CTCTTCAAAGGACCTGGTGG
2766
PRIMER_LEFT_10=49,20
2767
PRIMER_RIGHT_10=217,20
2768
PRIMER_LEFT_10_TM=60.423
2769
PRIMER_RIGHT_10_TM=59.691
2770
PRIMER_LEFT_10_GC_PERCENT=50.000
2771
PRIMER_RIGHT_10_GC_PERCENT=55.000
2772
PRIMER_LEFT_10_SELF_ANY=5.00
2773
PRIMER_RIGHT_10_SELF_ANY=5.00
2774
PRIMER_LEFT_10_SELF_END=1.00
2775
PRIMER_RIGHT_10_SELF_END=1.00
2776
PRIMER_LEFT_10_END_STABILITY=7.9000
2777
PRIMER_RIGHT_10_END_STABILITY=9.4000
2778
PRIMER_PAIR_10_COMPL_ANY=4.00
2779
PRIMER_PAIR_10_COMPL_END=3.00
2780
PRIMER_PRODUCT_SIZE_10=169
2781
PRIMER_PAIR_PENALTY_11=0.8345
2782
PRIMER_LEFT_11_PENALTY=0.525579
2783
PRIMER_RIGHT_11_PENALTY=0.308871
2784
PRIMER_LEFT_11_SEQUENCE=GGACCACGGATTATCCTTCC
2785
PRIMER_RIGHT_11_SEQUENCE=CTCTTCAAAGGACCTGGTGG
2786
PRIMER_LEFT_11=82,20
2787
PRIMER_RIGHT_11=217,20
2788
PRIMER_LEFT_11_TM=60.526
2789
PRIMER_RIGHT_11_TM=59.691
2790
PRIMER_LEFT_11_GC_PERCENT=55.000
2791
PRIMER_RIGHT_11_GC_PERCENT=55.000
2792
PRIMER_LEFT_11_SELF_ANY=7.00
2793
PRIMER_RIGHT_11_SELF_ANY=5.00
2794
PRIMER_LEFT_11_SELF_END=3.00
2795
PRIMER_RIGHT_11_SELF_END=1.00
2796
PRIMER_LEFT_11_END_STABILITY=8.2000
2797
PRIMER_RIGHT_11_END_STABILITY=9.4000
2798
PRIMER_PAIR_11_COMPL_ANY=5.00
2799
PRIMER_PAIR_11_COMPL_END=2.00
2800
PRIMER_PRODUCT_SIZE_11=136
2801
PRIMER_PAIR_PENALTY_12=0.9709
2802
PRIMER_LEFT_12_PENALTY=0.201280
2803
PRIMER_RIGHT_12_PENALTY=0.769636
2804
PRIMER_LEFT_12_SEQUENCE=TTTCCCCTTAGGACCAAGGT
2805
PRIMER_RIGHT_12_SEQUENCE=AAGGACCTGGTGGGACAGAT
2806
PRIMER_LEFT_12=53,20
2807
PRIMER_RIGHT_12=210,20
2808
PRIMER_LEFT_12_TM=59.799
2809
PRIMER_RIGHT_12_TM=60.770
2810
PRIMER_LEFT_12_GC_PERCENT=50.000
2811
PRIMER_RIGHT_12_GC_PERCENT=55.000
2812
PRIMER_LEFT_12_SELF_ANY=7.00
2813
PRIMER_RIGHT_12_SELF_ANY=5.00
2814
PRIMER_LEFT_12_SELF_END=5.00
2815
PRIMER_RIGHT_12_SELF_END=2.00
2816
PRIMER_LEFT_12_END_STABILITY=7.9000
2817
PRIMER_RIGHT_12_END_STABILITY=6.6000
2818
PRIMER_PAIR_12_COMPL_ANY=5.00
2819
PRIMER_PAIR_12_COMPL_END=1.00
2820
PRIMER_PRODUCT_SIZE_12=158
2821
PRIMER_PAIR_PENALTY_13=1.0196
2822
PRIMER_LEFT_13_PENALTY=0.249989
2823
PRIMER_RIGHT_13_PENALTY=0.769636
2824
PRIMER_LEFT_13_SEQUENCE=GACCACGGATTATCCTTCCA
2825
PRIMER_RIGHT_13_SEQUENCE=AAGGACCTGGTGGGACAGAT
2826
PRIMER_LEFT_13=83,20
2827
PRIMER_RIGHT_13=210,20
2828
PRIMER_LEFT_13_TM=59.750
2829
PRIMER_RIGHT_13_TM=60.770
2830
PRIMER_LEFT_13_GC_PERCENT=50.000
2831
PRIMER_RIGHT_13_GC_PERCENT=55.000
2832
PRIMER_LEFT_13_SELF_ANY=7.00
2833
PRIMER_RIGHT_13_SELF_ANY=5.00
2834
PRIMER_LEFT_13_SELF_END=1.00
2835
PRIMER_RIGHT_13_SELF_END=2.00
2836
PRIMER_LEFT_13_END_STABILITY=8.5000
2837
PRIMER_RIGHT_13_END_STABILITY=6.6000
2838
PRIMER_PAIR_13_COMPL_ANY=5.00
2839
PRIMER_PAIR_13_COMPL_END=4.00
2840
PRIMER_PRODUCT_SIZE_13=128
2841
PRIMER_PAIR_PENALTY_14=1.0446
2842
PRIMER_LEFT_14_PENALTY=1.026872
2843
PRIMER_RIGHT_14_PENALTY=0.017711
2844
PRIMER_LEFT_14_SEQUENCE=CCACGGATTATCCTTCCATTT
2845
PRIMER_RIGHT_14_SEQUENCE=CAGCTCTTCAAAGGACCTGG
2846
PRIMER_LEFT_14=85,21
2847
PRIMER_RIGHT_14=220,20
2848
PRIMER_LEFT_14_TM=60.027
2849
PRIMER_RIGHT_14_TM=59.982
2850
PRIMER_LEFT_14_GC_PERCENT=42.857
2851
PRIMER_RIGHT_14_GC_PERCENT=55.000
2852
PRIMER_LEFT_14_SELF_ANY=7.00
2853
PRIMER_RIGHT_14_SELF_ANY=6.00
2854
PRIMER_LEFT_14_SELF_END=0.00
2855
PRIMER_RIGHT_14_SELF_END=4.00
2856
PRIMER_LEFT_14_END_STABILITY=7.2000
2857
PRIMER_RIGHT_14_END_STABILITY=9.7000
2858
PRIMER_PAIR_14_COMPL_ANY=5.00
2859
PRIMER_PAIR_14_COMPL_END=2.00
2860
PRIMER_PRODUCT_SIZE_14=136
2861
PRIMER_PAIR_PENALTY_15=1.0492
2862
PRIMER_LEFT_15_PENALTY=1.031536
2863
PRIMER_RIGHT_15_PENALTY=0.017711
2864
PRIMER_LEFT_15_SEQUENCE=GACCACGGATTATCCTTCCAT
2865
PRIMER_RIGHT_15_SEQUENCE=CAGCTCTTCAAAGGACCTGG
2866
PRIMER_LEFT_15=83,21
2867
PRIMER_RIGHT_15=220,20
2868
PRIMER_LEFT_15_TM=60.032
2869
PRIMER_RIGHT_15_TM=59.982
2870
PRIMER_LEFT_15_GC_PERCENT=47.619
2871
PRIMER_RIGHT_15_GC_PERCENT=55.000
2872
PRIMER_LEFT_15_SELF_ANY=7.00
2873
PRIMER_RIGHT_15_SELF_ANY=6.00
2874
PRIMER_LEFT_15_SELF_END=2.00
2875
PRIMER_RIGHT_15_SELF_END=4.00
2876
PRIMER_LEFT_15_END_STABILITY=8.1000
2877
PRIMER_RIGHT_15_END_STABILITY=9.7000
2878
PRIMER_PAIR_15_COMPL_ANY=5.00
2879
PRIMER_PAIR_15_COMPL_END=2.00
2880
PRIMER_PRODUCT_SIZE_15=138
2881
PRIMER_PAIR_PENALTY_16=1.1169
2882
PRIMER_LEFT_16_PENALTY=1.099227
2883
PRIMER_RIGHT_16_PENALTY=0.017711
2884
PRIMER_LEFT_16_SEQUENCE=TCAGCTTTTTCCCCTTAGGA
2885
PRIMER_RIGHT_16_SEQUENCE=CAGCTCTTCAAAGGACCTGG
2886
PRIMER_LEFT_16=46,20
2887
PRIMER_RIGHT_16=220,20
2888
PRIMER_LEFT_16_TM=58.901
2889
PRIMER_RIGHT_16_TM=59.982
2890
PRIMER_LEFT_16_GC_PERCENT=45.000
2891
PRIMER_RIGHT_16_GC_PERCENT=55.000
2892
PRIMER_LEFT_16_SELF_ANY=5.00
2893
PRIMER_RIGHT_16_SELF_ANY=6.00
2894
PRIMER_LEFT_16_SELF_END=3.00
2895
PRIMER_RIGHT_16_SELF_END=4.00
2896
PRIMER_LEFT_16_END_STABILITY=7.2000
2897
PRIMER_RIGHT_16_END_STABILITY=9.7000
2898
PRIMER_PAIR_16_COMPL_ANY=4.00
2899
PRIMER_PAIR_16_COMPL_END=0.00
2900
PRIMER_PRODUCT_SIZE_16=175
2901
PRIMER_PAIR_PENALTY_17=1.1538
2902
PRIMER_LEFT_17_PENALTY=1.136051
2903
PRIMER_RIGHT_17_PENALTY=0.017711
2904
PRIMER_LEFT_17_SEQUENCE=GGTATAGCCTTAGGACCACGG
2905
PRIMER_RIGHT_17_SEQUENCE=CAGCTCTTCAAAGGACCTGG
2906
PRIMER_LEFT_17=70,21
2907
PRIMER_RIGHT_17=220,20
2908
PRIMER_LEFT_17_TM=59.864
2909
PRIMER_RIGHT_17_TM=59.982
2910
PRIMER_LEFT_17_GC_PERCENT=57.143
2911
PRIMER_RIGHT_17_GC_PERCENT=55.000
2912
PRIMER_LEFT_17_SELF_ANY=5.00
2913
PRIMER_RIGHT_17_SELF_ANY=6.00
2914
PRIMER_LEFT_17_SELF_END=3.00
2915
PRIMER_RIGHT_17_SELF_END=4.00
2916
PRIMER_LEFT_17_END_STABILITY=9.9000
2917
PRIMER_RIGHT_17_END_STABILITY=9.7000
2918
PRIMER_PAIR_17_COMPL_ANY=4.00
2919
PRIMER_PAIR_17_COMPL_END=3.00
2920
PRIMER_PRODUCT_SIZE_17=151
2921
PRIMER_PAIR_PENALTY_18=1.1829
2922
PRIMER_LEFT_18_PENALTY=1.026872
2923
PRIMER_RIGHT_18_PENALTY=0.156061
2924
PRIMER_LEFT_18_SEQUENCE=CCACGGATTATCCTTCCATTT
2925
PRIMER_RIGHT_18_SEQUENCE=GCTCTTCAAAGGACCTGGTG
2926
PRIMER_LEFT_18=85,21
2927
PRIMER_RIGHT_18=218,20
2928
PRIMER_LEFT_18_TM=60.027
2929
PRIMER_RIGHT_18_TM=59.844
2930
PRIMER_LEFT_18_GC_PERCENT=42.857
2931
PRIMER_RIGHT_18_GC_PERCENT=55.000
2932
PRIMER_LEFT_18_SELF_ANY=7.00
2933
PRIMER_RIGHT_18_SELF_ANY=5.00
2934
PRIMER_LEFT_18_SELF_END=0.00
2935
PRIMER_RIGHT_18_SELF_END=3.00
2936
PRIMER_LEFT_18_END_STABILITY=7.2000
2937
PRIMER_RIGHT_18_END_STABILITY=8.2000
2938
PRIMER_PAIR_18_COMPL_ANY=5.00
2939
PRIMER_PAIR_18_COMPL_END=0.00
2940
PRIMER_PRODUCT_SIZE_18=134
2941
PRIMER_PAIR_PENALTY_19=1.1876
2942
PRIMER_LEFT_19_PENALTY=1.031536
2943
PRIMER_RIGHT_19_PENALTY=0.156061
2944
PRIMER_LEFT_19_SEQUENCE=GACCACGGATTATCCTTCCAT
2945
PRIMER_RIGHT_19_SEQUENCE=GCTCTTCAAAGGACCTGGTG
2946
PRIMER_LEFT_19=83,21
2947
PRIMER_RIGHT_19=218,20
2948
PRIMER_LEFT_19_TM=60.032
2949
PRIMER_RIGHT_19_TM=59.844
2950
PRIMER_LEFT_19_GC_PERCENT=47.619
2951
PRIMER_RIGHT_19_GC_PERCENT=55.000
2952
PRIMER_LEFT_19_SELF_ANY=7.00
2953
PRIMER_RIGHT_19_SELF_ANY=5.00
2954
PRIMER_LEFT_19_SELF_END=2.00
2955
PRIMER_RIGHT_19_SELF_END=3.00
2956
PRIMER_LEFT_19_END_STABILITY=8.1000
2957
PRIMER_RIGHT_19_END_STABILITY=8.2000
2958
PRIMER_PAIR_19_COMPL_ANY=5.00
2959
PRIMER_PAIR_19_COMPL_END=1.00
2960
PRIMER_PRODUCT_SIZE_19=136
2961
PRIMER_PAIR_PENALTY_20=1.1925
2962
PRIMER_LEFT_20_PENALTY=0.422888
2963
PRIMER_RIGHT_20_PENALTY=0.769636
2964
PRIMER_LEFT_20_SEQUENCE=GCTTTTTCCCCTTAGGACCA
2965
PRIMER_RIGHT_20_SEQUENCE=AAGGACCTGGTGGGACAGAT
2966
PRIMER_LEFT_20=49,20
2967
PRIMER_RIGHT_20=210,20
2968
PRIMER_LEFT_20_TM=60.423
2969
PRIMER_RIGHT_20_TM=60.770
2970
PRIMER_LEFT_20_GC_PERCENT=50.000
2971
PRIMER_RIGHT_20_GC_PERCENT=55.000
2972
PRIMER_LEFT_20_SELF_ANY=5.00
2973
PRIMER_RIGHT_20_SELF_ANY=5.00
2974
PRIMER_LEFT_20_SELF_END=1.00
2975
PRIMER_RIGHT_20_SELF_END=2.00
2976
PRIMER_LEFT_20_END_STABILITY=7.9000
2977
PRIMER_RIGHT_20_END_STABILITY=6.6000
2978
PRIMER_PAIR_20_COMPL_ANY=5.00
2979
PRIMER_PAIR_20_COMPL_END=3.00
2980
PRIMER_PRODUCT_SIZE_20=162
2981
PRIMER_PAIR_PENALTY_21=1.2017
2982
PRIMER_LEFT_21_PENALTY=1.184005
2983
PRIMER_RIGHT_21_PENALTY=0.017711
2984
PRIMER_LEFT_21_SEQUENCE=TTTCCCCTTAGGACCAAGGTA
2985
PRIMER_RIGHT_21_SEQUENCE=CAGCTCTTCAAAGGACCTGG
2986
PRIMER_LEFT_21=53,21
2987
PRIMER_RIGHT_21=220,20
2988
PRIMER_LEFT_21_TM=59.816
2989
PRIMER_RIGHT_21_TM=59.982
2990
PRIMER_LEFT_21_GC_PERCENT=47.619
2991
PRIMER_RIGHT_21_GC_PERCENT=55.000
2992
PRIMER_LEFT_21_SELF_ANY=7.00
2993
PRIMER_RIGHT_21_SELF_ANY=6.00
2994
PRIMER_LEFT_21_SELF_END=3.00
2995
PRIMER_RIGHT_21_SELF_END=4.00
2996
PRIMER_LEFT_21_END_STABILITY=6.9000
2997
PRIMER_RIGHT_21_END_STABILITY=9.7000
2998
PRIMER_PAIR_21_COMPL_ANY=4.00
2999
PRIMER_PAIR_21_COMPL_END=3.00
3000
PRIMER_PRODUCT_SIZE_21=168
3001
PRIMER_PAIR_PENALTY_22=1.2054
3002
PRIMER_LEFT_22_PENALTY=1.187663
3003
PRIMER_RIGHT_22_PENALTY=0.017711
3004
PRIMER_LEFT_22_SEQUENCE=GCCTTAGGACCACGGATTATC
3005
PRIMER_RIGHT_22_SEQUENCE=CAGCTCTTCAAAGGACCTGG
3006
PRIMER_LEFT_22=76,21
3007
PRIMER_RIGHT_22=220,20
3008
PRIMER_LEFT_22_TM=59.812
3009
PRIMER_RIGHT_22_TM=59.982
3010
PRIMER_LEFT_22_GC_PERCENT=52.381
3011
PRIMER_RIGHT_22_GC_PERCENT=55.000
3012
PRIMER_LEFT_22_SELF_ANY=5.00
3013
PRIMER_RIGHT_22_SELF_ANY=6.00
3014
PRIMER_LEFT_22_SELF_END=5.00
3015
PRIMER_RIGHT_22_SELF_END=4.00
3016
PRIMER_LEFT_22_END_STABILITY=5.9000
3017
PRIMER_RIGHT_22_END_STABILITY=9.7000
3018
PRIMER_PAIR_22_COMPL_ANY=4.00
3019
PRIMER_PAIR_22_COMPL_END=1.00
3020
PRIMER_PRODUCT_SIZE_22=145
3021
PRIMER_PAIR_PENALTY_23=1.2553
3022
PRIMER_LEFT_23_PENALTY=1.099227
3023
PRIMER_RIGHT_23_PENALTY=0.156061
3024
PRIMER_LEFT_23_SEQUENCE=TCAGCTTTTTCCCCTTAGGA
3025
PRIMER_RIGHT_23_SEQUENCE=GCTCTTCAAAGGACCTGGTG
3026
PRIMER_LEFT_23=46,20
3027
PRIMER_RIGHT_23=218,20
3028
PRIMER_LEFT_23_TM=58.901
3029
PRIMER_RIGHT_23_TM=59.844
3030
PRIMER_LEFT_23_GC_PERCENT=45.000
3031
PRIMER_RIGHT_23_GC_PERCENT=55.000
3032
PRIMER_LEFT_23_SELF_ANY=5.00
3033
PRIMER_RIGHT_23_SELF_ANY=5.00
3034
PRIMER_LEFT_23_SELF_END=3.00
3035
PRIMER_RIGHT_23_SELF_END=3.00
3036
PRIMER_LEFT_23_END_STABILITY=7.2000
3037
PRIMER_RIGHT_23_END_STABILITY=8.2000
3038
PRIMER_PAIR_23_COMPL_ANY=4.00
3039
PRIMER_PAIR_23_COMPL_END=0.00
3040
PRIMER_PRODUCT_SIZE_23=173
3041
PRIMER_PAIR_PENALTY_24=1.2554
3042
PRIMER_LEFT_24_PENALTY=0.201280
3043
PRIMER_RIGHT_24_PENALTY=1.054163
3044
PRIMER_LEFT_24_SEQUENCE=TTTCCCCTTAGGACCAAGGT
3045
PRIMER_RIGHT_24_SEQUENCE=AAAGGACCTGGTGGGACAG
3046
PRIMER_LEFT_24=53,20
3047
PRIMER_RIGHT_24=211,19
3048
PRIMER_LEFT_24_TM=59.799
3049
PRIMER_RIGHT_24_TM=59.946
3050
PRIMER_LEFT_24_GC_PERCENT=50.000
3051
PRIMER_RIGHT_24_GC_PERCENT=57.895
3052
PRIMER_LEFT_24_SELF_ANY=7.00
3053
PRIMER_RIGHT_24_SELF_ANY=5.00
3054
PRIMER_LEFT_24_SELF_END=5.00
3055
PRIMER_RIGHT_24_SELF_END=1.00
3056
PRIMER_LEFT_24_END_STABILITY=7.9000
3057
PRIMER_RIGHT_24_END_STABILITY=6.4000
3058
PRIMER_PAIR_24_COMPL_ANY=5.00
3059
PRIMER_PAIR_24_COMPL_END=3.00
3060
PRIMER_PRODUCT_SIZE_24=159
3061
PRIMER_PAIR_PENALTY_25=1.2921
3062
PRIMER_LEFT_25_PENALTY=1.136051
3063
PRIMER_RIGHT_25_PENALTY=0.156061
3064
PRIMER_LEFT_25_SEQUENCE=GGTATAGCCTTAGGACCACGG
3065
PRIMER_RIGHT_25_SEQUENCE=GCTCTTCAAAGGACCTGGTG
3066
PRIMER_LEFT_25=70,21
3067
PRIMER_RIGHT_25=218,20
3068
PRIMER_LEFT_25_TM=59.864
3069
PRIMER_RIGHT_25_TM=59.844
3070
PRIMER_LEFT_25_GC_PERCENT=57.143
3071
PRIMER_RIGHT_25_GC_PERCENT=55.000
3072
PRIMER_LEFT_25_SELF_ANY=5.00
3073
PRIMER_RIGHT_25_SELF_ANY=5.00
3074
PRIMER_LEFT_25_SELF_END=3.00
3075
PRIMER_RIGHT_25_SELF_END=3.00
3076
PRIMER_LEFT_25_END_STABILITY=9.9000
3077
PRIMER_RIGHT_25_END_STABILITY=8.2000
3078
PRIMER_PAIR_25_COMPL_ANY=4.00
3079
PRIMER_PAIR_25_COMPL_END=3.00
3080
PRIMER_PRODUCT_SIZE_25=149
3081
PRIMER_PAIR_PENALTY_26=1.2952
3082
PRIMER_LEFT_26_PENALTY=0.525579
3083
PRIMER_RIGHT_26_PENALTY=0.769636
3084
PRIMER_LEFT_26_SEQUENCE=GGACCACGGATTATCCTTCC
3085
PRIMER_RIGHT_26_SEQUENCE=AAGGACCTGGTGGGACAGAT
3086
PRIMER_LEFT_26=82,20
3087
PRIMER_RIGHT_26=210,20
3088
PRIMER_LEFT_26_TM=60.526
3089
PRIMER_RIGHT_26_TM=60.770
3090
PRIMER_LEFT_26_GC_PERCENT=55.000
3091
PRIMER_RIGHT_26_GC_PERCENT=55.000
3092
PRIMER_LEFT_26_SELF_ANY=7.00
3093
PRIMER_RIGHT_26_SELF_ANY=5.00
3094
PRIMER_LEFT_26_SELF_END=3.00
3095
PRIMER_RIGHT_26_SELF_END=2.00
3096
PRIMER_LEFT_26_END_STABILITY=8.2000
3097
PRIMER_RIGHT_26_END_STABILITY=6.6000
3098
PRIMER_PAIR_26_COMPL_ANY=5.00
3099
PRIMER_PAIR_26_COMPL_END=4.00
3100
PRIMER_PRODUCT_SIZE_26=129
3101
PRIMER_PAIR_PENALTY_27=1.3030
3102
PRIMER_LEFT_27_PENALTY=1.285273
3103
PRIMER_RIGHT_27_PENALTY=0.017711
3104
PRIMER_LEFT_27_SEQUENCE=CCACGGATTATCCTTCCATT
3105
PRIMER_RIGHT_27_SEQUENCE=CAGCTCTTCAAAGGACCTGG
3106
PRIMER_LEFT_27=85,20
3107
PRIMER_RIGHT_27=220,20
3108
PRIMER_LEFT_27_TM=58.715
3109
PRIMER_RIGHT_27_TM=59.982
3110
PRIMER_LEFT_27_GC_PERCENT=45.000
3111
PRIMER_RIGHT_27_GC_PERCENT=55.000
3112
PRIMER_LEFT_27_SELF_ANY=7.00
3113
PRIMER_RIGHT_27_SELF_ANY=6.00
3114
PRIMER_LEFT_27_SELF_END=1.00
3115
PRIMER_RIGHT_27_SELF_END=4.00
3116
PRIMER_LEFT_27_END_STABILITY=8.4000
3117
PRIMER_RIGHT_27_END_STABILITY=9.7000
3118
PRIMER_PAIR_27_COMPL_ANY=5.00
3119
PRIMER_PAIR_27_COMPL_END=1.00
3120
PRIMER_PRODUCT_SIZE_27=136
3121
PRIMER_PAIR_PENALTY_28=1.3042
3122
PRIMER_LEFT_28_PENALTY=0.249989
3123
PRIMER_RIGHT_28_PENALTY=1.054163
3124
PRIMER_LEFT_28_SEQUENCE=GACCACGGATTATCCTTCCA
3125
PRIMER_RIGHT_28_SEQUENCE=AAAGGACCTGGTGGGACAG
3126
PRIMER_LEFT_28=83,20
3127
PRIMER_RIGHT_28=211,19
3128
PRIMER_LEFT_28_TM=59.750
3129
PRIMER_RIGHT_28_TM=59.946
3130
PRIMER_LEFT_28_GC_PERCENT=50.000
3131
PRIMER_RIGHT_28_GC_PERCENT=57.895
3132
PRIMER_LEFT_28_SELF_ANY=7.00
3133
PRIMER_RIGHT_28_SELF_ANY=5.00
3134
PRIMER_LEFT_28_SELF_END=1.00
3135
PRIMER_RIGHT_28_SELF_END=1.00
3136
PRIMER_LEFT_28_END_STABILITY=8.5000
3137
PRIMER_RIGHT_28_END_STABILITY=6.4000
3138
PRIMER_PAIR_28_COMPL_ANY=5.00
3139
PRIMER_PAIR_28_COMPL_END=3.00
3140
PRIMER_PRODUCT_SIZE_28=129
3141
PRIMER_PAIR_PENALTY_29=1.3055
3142
PRIMER_LEFT_29_PENALTY=1.287819
3143
PRIMER_RIGHT_29_PENALTY=0.017711
3144
PRIMER_LEFT_29_SEQUENCE=AGCTTTTTCCCCTTAGGACC
3145
PRIMER_RIGHT_29_SEQUENCE=CAGCTCTTCAAAGGACCTGG
3146
PRIMER_LEFT_29=48,20
3147
PRIMER_RIGHT_29=220,20
3148
PRIMER_LEFT_29_TM=58.712
3149
PRIMER_RIGHT_29_TM=59.982
3150
PRIMER_LEFT_29_GC_PERCENT=50.000
3151
PRIMER_RIGHT_29_GC_PERCENT=55.000
3152
PRIMER_LEFT_29_SELF_ANY=5.00
3153
PRIMER_RIGHT_29_SELF_ANY=6.00
3154
PRIMER_LEFT_29_SELF_END=3.00
3155
PRIMER_RIGHT_29_SELF_END=4.00
3156
PRIMER_LEFT_29_END_STABILITY=9.1000
3157
PRIMER_RIGHT_29_END_STABILITY=9.7000
3158
PRIMER_PAIR_29_COMPL_ANY=4.00
3159
PRIMER_PAIR_29_COMPL_END=2.00
3160
PRIMER_PRODUCT_SIZE_29=173
3161
PRIMER_PAIR_PENALTY_30=1.3059
3162
PRIMER_LEFT_30_PENALTY=1.288173
3163
PRIMER_RIGHT_30_PENALTY=0.017711
3164
PRIMER_LEFT_30_SEQUENCE=TCAGCTTTTTCCCCTTAGGAC
3165
PRIMER_RIGHT_30_SEQUENCE=CAGCTCTTCAAAGGACCTGG
3166
PRIMER_LEFT_30=46,21
3167
PRIMER_RIGHT_30=220,20
3168
PRIMER_LEFT_30_TM=59.712
3169
PRIMER_RIGHT_30_TM=59.982
3170
PRIMER_LEFT_30_GC_PERCENT=47.619
3171
PRIMER_RIGHT_30_GC_PERCENT=55.000
3172
PRIMER_LEFT_30_SELF_ANY=5.00
3173
PRIMER_RIGHT_30_SELF_ANY=6.00
3174
PRIMER_LEFT_30_SELF_END=1.00
3175
PRIMER_RIGHT_30_SELF_END=4.00
3176
PRIMER_LEFT_30_END_STABILITY=7.6000
3177
PRIMER_RIGHT_30_END_STABILITY=9.7000
3178
PRIMER_PAIR_30_COMPL_ANY=4.00
3179
PRIMER_PAIR_30_COMPL_END=1.00
3180
PRIMER_PRODUCT_SIZE_30=175
3181
PRIMER_PAIR_PENALTY_31=1.3357
3182
PRIMER_LEFT_31_PENALTY=1.026872
3183
PRIMER_RIGHT_31_PENALTY=0.308871
3184
PRIMER_LEFT_31_SEQUENCE=CCACGGATTATCCTTCCATTT
3185
PRIMER_RIGHT_31_SEQUENCE=CTCTTCAAAGGACCTGGTGG
3186
PRIMER_LEFT_31=85,21
3187
PRIMER_RIGHT_31=217,20
3188
PRIMER_LEFT_31_TM=60.027
3189
PRIMER_RIGHT_31_TM=59.691
3190
PRIMER_LEFT_31_GC_PERCENT=42.857
3191
PRIMER_RIGHT_31_GC_PERCENT=55.000
3192
PRIMER_LEFT_31_SELF_ANY=7.00
3193
PRIMER_RIGHT_31_SELF_ANY=5.00
3194
PRIMER_LEFT_31_SELF_END=0.00
3195
PRIMER_RIGHT_31_SELF_END=1.00
3196
PRIMER_LEFT_31_END_STABILITY=7.2000
3197
PRIMER_RIGHT_31_END_STABILITY=9.4000
3198
PRIMER_PAIR_31_COMPL_ANY=5.00
3199
PRIMER_PAIR_31_COMPL_END=1.00
3200
PRIMER_PRODUCT_SIZE_31=133
3201
PRIMER_PAIR_PENALTY_32=1.3393
3202
PRIMER_LEFT_32_PENALTY=1.183236
3203
PRIMER_RIGHT_32_PENALTY=0.156061
3204
PRIMER_LEFT_32_SEQUENCE=TTCAGCTTTTTCCCCTTAGGA
3205
PRIMER_RIGHT_32_SEQUENCE=GCTCTTCAAAGGACCTGGTG
3206
PRIMER_LEFT_32=45,21
3207
PRIMER_RIGHT_32=218,20
3208
PRIMER_LEFT_32_TM=60.183
3209
PRIMER_RIGHT_32_TM=59.844
3210
PRIMER_LEFT_32_GC_PERCENT=42.857
3211
PRIMER_RIGHT_32_GC_PERCENT=55.000
3212
PRIMER_LEFT_32_SELF_ANY=5.00
3213
PRIMER_RIGHT_32_SELF_ANY=5.00
3214
PRIMER_LEFT_32_SELF_END=3.00
3215
PRIMER_RIGHT_32_SELF_END=3.00
3216
PRIMER_LEFT_32_END_STABILITY=7.2000
3217
PRIMER_RIGHT_32_END_STABILITY=8.2000
3218
PRIMER_PAIR_32_COMPL_ANY=4.00
3219
PRIMER_PAIR_32_COMPL_END=0.00
3220
PRIMER_PRODUCT_SIZE_32=174
3221
PRIMER_PAIR_PENALTY_33=1.3401
3222
PRIMER_LEFT_33_PENALTY=1.184005
3223
PRIMER_RIGHT_33_PENALTY=0.156061
3224
PRIMER_LEFT_33_SEQUENCE=TTTCCCCTTAGGACCAAGGTA
3225
PRIMER_RIGHT_33_SEQUENCE=GCTCTTCAAAGGACCTGGTG
3226
PRIMER_LEFT_33=53,21
3227
PRIMER_RIGHT_33=218,20
3228
PRIMER_LEFT_33_TM=59.816
3229
PRIMER_RIGHT_33_TM=59.844
3230
PRIMER_LEFT_33_GC_PERCENT=47.619
3231
PRIMER_RIGHT_33_GC_PERCENT=55.000
3232
PRIMER_LEFT_33_SELF_ANY=7.00
3233
PRIMER_RIGHT_33_SELF_ANY=5.00
3234
PRIMER_LEFT_33_SELF_END=3.00
3235
PRIMER_RIGHT_33_SELF_END=3.00
3236
PRIMER_LEFT_33_END_STABILITY=6.9000
3237
PRIMER_RIGHT_33_END_STABILITY=8.2000
3238
PRIMER_PAIR_33_COMPL_ANY=5.00
3239
PRIMER_PAIR_33_COMPL_END=3.00
3240
PRIMER_PRODUCT_SIZE_33=166
3241
PRIMER_PAIR_PENALTY_34=1.3404
3242
PRIMER_LEFT_34_PENALTY=1.031536
3243
PRIMER_RIGHT_34_PENALTY=0.308871
3244
PRIMER_LEFT_34_SEQUENCE=GACCACGGATTATCCTTCCAT
3245
PRIMER_RIGHT_34_SEQUENCE=CTCTTCAAAGGACCTGGTGG
3246
PRIMER_LEFT_34=83,21
3247
PRIMER_RIGHT_34=217,20
3248
PRIMER_LEFT_34_TM=60.032
3249
PRIMER_RIGHT_34_TM=59.691
3250
PRIMER_LEFT_34_GC_PERCENT=47.619
3251
PRIMER_RIGHT_34_GC_PERCENT=55.000
3252
PRIMER_LEFT_34_SELF_ANY=7.00
3253
PRIMER_RIGHT_34_SELF_ANY=5.00
3254
PRIMER_LEFT_34_SELF_END=2.00
3255
PRIMER_RIGHT_34_SELF_END=1.00
3256
PRIMER_LEFT_34_END_STABILITY=8.1000
3257
PRIMER_RIGHT_34_END_STABILITY=9.4000
3258
PRIMER_PAIR_34_COMPL_ANY=5.00
3259
PRIMER_PAIR_34_COMPL_END=2.00
3260
PRIMER_PRODUCT_SIZE_34=135
3261
PRIMER_PAIR_PENALTY_35=1.3437
3262
PRIMER_LEFT_35_PENALTY=1.187663
3263
PRIMER_RIGHT_35_PENALTY=0.156061
3264
PRIMER_LEFT_35_SEQUENCE=GCCTTAGGACCACGGATTATC
3265
PRIMER_RIGHT_35_SEQUENCE=GCTCTTCAAAGGACCTGGTG
3266
PRIMER_LEFT_35=76,21
3267
PRIMER_RIGHT_35=218,20
3268
PRIMER_LEFT_35_TM=59.812
3269
PRIMER_RIGHT_35_TM=59.844
3270
PRIMER_LEFT_35_GC_PERCENT=52.381
3271
PRIMER_RIGHT_35_GC_PERCENT=55.000
3272
PRIMER_LEFT_35_SELF_ANY=5.00
3273
PRIMER_RIGHT_35_SELF_ANY=5.00
3274
PRIMER_LEFT_35_SELF_END=5.00
3275
PRIMER_RIGHT_35_SELF_END=3.00
3276
PRIMER_LEFT_35_END_STABILITY=5.9000
3277
PRIMER_RIGHT_35_END_STABILITY=8.2000
3278
PRIMER_PAIR_35_COMPL_ANY=4.00
3279
PRIMER_PAIR_35_COMPL_END=1.00
3280
PRIMER_PRODUCT_SIZE_35=143
3281
PRIMER_PAIR_PENALTY_36=1.3471
3282
PRIMER_LEFT_36_PENALTY=1.329419
3283
PRIMER_RIGHT_36_PENALTY=0.017711
3284
PRIMER_LEFT_36_SEQUENCE=AGCCTTAGGACCACGGATTA
3285
PRIMER_RIGHT_36_SEQUENCE=CAGCTCTTCAAAGGACCTGG
3286
PRIMER_LEFT_36=75,20
3287
PRIMER_RIGHT_36=220,20
3288
PRIMER_LEFT_36_TM=58.671
3289
PRIMER_RIGHT_36_TM=59.982
3290
PRIMER_LEFT_36_GC_PERCENT=50.000
3291
PRIMER_RIGHT_36_GC_PERCENT=55.000
3292
PRIMER_LEFT_36_SELF_ANY=5.00
3293
PRIMER_RIGHT_36_SELF_ANY=6.00
3294
PRIMER_LEFT_36_SELF_END=2.00
3295
PRIMER_RIGHT_36_SELF_END=4.00
3296
PRIMER_LEFT_36_END_STABILITY=5.9000
3297
PRIMER_RIGHT_36_END_STABILITY=9.7000
3298
PRIMER_PAIR_36_COMPL_ANY=4.00
3299
PRIMER_PAIR_36_COMPL_END=0.00
3300
PRIMER_PRODUCT_SIZE_36=146
3301
PRIMER_PAIR_PENALTY_37=1.3471
3302
PRIMER_LEFT_37_PENALTY=1.329419
3303
PRIMER_RIGHT_37_PENALTY=0.017711
3304
PRIMER_LEFT_37_SEQUENCE=TAGCCTTAGGACCACGGATT
3305
PRIMER_RIGHT_37_SEQUENCE=CAGCTCTTCAAAGGACCTGG
3306
PRIMER_LEFT_37=74,20
3307
PRIMER_RIGHT_37=220,20
3308
PRIMER_LEFT_37_TM=58.671
3309
PRIMER_RIGHT_37_TM=59.982
3310
PRIMER_LEFT_37_GC_PERCENT=50.000
3311
PRIMER_RIGHT_37_GC_PERCENT=55.000
3312
PRIMER_LEFT_37_SELF_ANY=5.00
3313
PRIMER_RIGHT_37_SELF_ANY=6.00
3314
PRIMER_LEFT_37_SELF_END=1.00
3315
PRIMER_RIGHT_37_SELF_END=4.00
3316
PRIMER_LEFT_37_END_STABILITY=8.1000
3317
PRIMER_RIGHT_37_END_STABILITY=9.7000
3318
PRIMER_PAIR_37_COMPL_ANY=4.00
3319
PRIMER_PAIR_37_COMPL_END=1.00
3320
PRIMER_PRODUCT_SIZE_37=147
3321
PRIMER_PAIR_PENALTY_38=1.4081
3322
PRIMER_LEFT_38_PENALTY=1.099227
3323
PRIMER_RIGHT_38_PENALTY=0.308871
3324
PRIMER_LEFT_38_SEQUENCE=TCAGCTTTTTCCCCTTAGGA
3325
PRIMER_RIGHT_38_SEQUENCE=CTCTTCAAAGGACCTGGTGG
3326
PRIMER_LEFT_38=46,20
3327
PRIMER_RIGHT_38=217,20
3328
PRIMER_LEFT_38_TM=58.901
3329
PRIMER_RIGHT_38_TM=59.691
3330
PRIMER_LEFT_38_GC_PERCENT=45.000
3331
PRIMER_RIGHT_38_GC_PERCENT=55.000
3332
PRIMER_LEFT_38_SELF_ANY=5.00
3333
PRIMER_RIGHT_38_SELF_ANY=5.00
3334
PRIMER_LEFT_38_SELF_END=3.00
3335
PRIMER_RIGHT_38_SELF_END=1.00
3336
PRIMER_LEFT_38_END_STABILITY=7.2000
3337
PRIMER_RIGHT_38_END_STABILITY=9.4000
3338
PRIMER_PAIR_38_COMPL_ANY=4.00
3339
PRIMER_PAIR_38_COMPL_END=1.00
3340
PRIMER_PRODUCT_SIZE_38=172
3341
PRIMER_PAIR_PENALTY_39=1.4413
3342
PRIMER_LEFT_39_PENALTY=1.285273
3343
PRIMER_RIGHT_39_PENALTY=0.156061
3344
PRIMER_LEFT_39_SEQUENCE=CCACGGATTATCCTTCCATT
3345
PRIMER_RIGHT_39_SEQUENCE=GCTCTTCAAAGGACCTGGTG
3346
PRIMER_LEFT_39=85,20
3347
PRIMER_RIGHT_39=218,20
3348
PRIMER_LEFT_39_TM=58.715
3349
PRIMER_RIGHT_39_TM=59.844
3350
PRIMER_LEFT_39_GC_PERCENT=45.000
3351
PRIMER_RIGHT_39_GC_PERCENT=55.000
3352
PRIMER_LEFT_39_SELF_ANY=7.00
3353
PRIMER_RIGHT_39_SELF_ANY=5.00
3354
PRIMER_LEFT_39_SELF_END=1.00
3355
PRIMER_RIGHT_39_SELF_END=3.00
3356
PRIMER_LEFT_39_END_STABILITY=8.4000
3357
PRIMER_RIGHT_39_END_STABILITY=8.2000
3358
PRIMER_PAIR_39_COMPL_ANY=5.00
3359
PRIMER_PAIR_39_COMPL_END=0.00
3360
PRIMER_PRODUCT_SIZE_39=134
3361
=
3362
PRIMER_NUM_RETURN=8
3363
MARKER_NAME=09.RHAP33FA9.seq
3364
SEQUENCE=GCCTTGGGATTTTACTTATTGAGACATCCTGTTACCATGTAGGAGTTTAGAGAGGTTTCATTTTCTCTGCTAAGTAATGAGTTCAAAGCCAGGAAATCATCTCAGAGACAGCAGGCAGCAATAAGAAACACATTTTATTTCAGTTTATGAANNNNNNNNNNNNNNNNNNAGGGGGGAGGGAGGGAGGGACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCNNNNNNNNNNNNNNNNNCANNNCNCNCTGNAGCANAANCNCCCTGCAAGNGCCGNCGNNGTNNCNGCNNTNNNNTCCCCCNGCNGGNGNNCCGNGCNCCCNNCCGGCGNNNCNNCNTGCNGNNG
3365
INCLUDED_REGION=0,371
3366
TARGET=151,18,AC
3367
TARGET=192,53,GA
3368
TARGET=248,16,AG
3369
PRIMER_PAIR_PENALTY=8.2998
3370
PRIMER_LEFT_PENALTY=4.294975
3371
PRIMER_RIGHT_PENALTY=4.004782
3372
PRIMER_LEFT_SEQUENCE=TGAGACATCCTGTTACCATGTAGG
3373
PRIMER_RIGHT_SEQUENCE=CTGTCCCTCCCTCCCTCC
3374
PRIMER_LEFT=19,24
3375
PRIMER_RIGHT=191,18
3376
PRIMER_LEFT_TM=60.295
3377
PRIMER_RIGHT_TM=62.005
3378
PRIMER_LEFT_GC_PERCENT=45.833
3379
PRIMER_RIGHT_GC_PERCENT=72.222
3380
PRIMER_LEFT_SELF_ANY=4.00
3381
PRIMER_RIGHT_SELF_ANY=1.00
3382
PRIMER_LEFT_SELF_END=2.00
3383
PRIMER_RIGHT_SELF_END=0.00
3384
PRIMER_LEFT_END_STABILITY=6.9000
3385
PRIMER_RIGHT_END_STABILITY=9.4000
3386
PRIMER_PAIR_COMPL_ANY=4.00
3387
PRIMER_PAIR_COMPL_END=3.00
3388
PRIMER_PRODUCT_SIZE=173
3389
PRIMER_PAIR_PENALTY_1=8.3060
3390
PRIMER_LEFT_1_PENALTY=4.301260
3391
PRIMER_RIGHT_1_PENALTY=4.004782
3392
PRIMER_LEFT_1_SEQUENCE=TGCTAAGTAATGAGTTCAAAGCCA
3393
PRIMER_RIGHT_1_SEQUENCE=CTGTCCCTCCCTCCCTCC
3394
PRIMER_LEFT_1=67,24
3395
PRIMER_RIGHT_1=191,18
3396
PRIMER_LEFT_1_TM=60.301
3397
PRIMER_RIGHT_1_TM=62.005
3398
PRIMER_LEFT_1_GC_PERCENT=37.500
3399
PRIMER_RIGHT_1_GC_PERCENT=72.222
3400
PRIMER_LEFT_1_SELF_ANY=4.00
3401
PRIMER_RIGHT_1_SELF_ANY=1.00
3402
PRIMER_LEFT_1_SELF_END=2.00
3403
PRIMER_RIGHT_1_SELF_END=0.00
3404
PRIMER_LEFT_1_END_STABILITY=9.7000
3405
PRIMER_RIGHT_1_END_STABILITY=9.4000
3406
PRIMER_PAIR_1_COMPL_ANY=3.00
3407
PRIMER_PAIR_1_COMPL_END=0.00
3408
PRIMER_PRODUCT_SIZE_1=125
3409
PRIMER_PAIR_PENALTY_2=8.5894
3410
PRIMER_LEFT_2_PENALTY=4.584629
3411
PRIMER_RIGHT_2_PENALTY=4.004782
3412
PRIMER_LEFT_2_SEQUENCE=GAGACATCCTGTTACCATGTAGGA
3413
PRIMER_RIGHT_2_SEQUENCE=CTGTCCCTCCCTCCCTCC
3414
PRIMER_LEFT_2=20,24
3415
PRIMER_RIGHT_2=191,18
3416
PRIMER_LEFT_2_TM=59.415
3417
PRIMER_RIGHT_2_TM=62.005
3418
PRIMER_LEFT_2_GC_PERCENT=45.833
3419
PRIMER_RIGHT_2_GC_PERCENT=72.222
3420
PRIMER_LEFT_2_SELF_ANY=4.00
3421
PRIMER_RIGHT_2_SELF_ANY=1.00
3422
PRIMER_LEFT_2_SELF_END=2.00
3423
PRIMER_RIGHT_2_SELF_END=0.00
3424
PRIMER_LEFT_2_END_STABILITY=7.2000
3425
PRIMER_RIGHT_2_END_STABILITY=9.4000
3426
PRIMER_PAIR_2_COMPL_ANY=4.00
3427
PRIMER_PAIR_2_COMPL_END=3.00
3428
PRIMER_PRODUCT_SIZE_2=172
3429
PRIMER_PAIR_PENALTY_3=8.9567
3430
PRIMER_LEFT_3_PENALTY=4.951910
3431
PRIMER_RIGHT_3_PENALTY=4.004782
3432
PRIMER_LEFT_3_SEQUENCE=TTGAGACATCCTGTTACCATGT
3433
PRIMER_RIGHT_3_SEQUENCE=CTGTCCCTCCCTCCCTCC
3434
PRIMER_LEFT_3=18,22
3435
PRIMER_RIGHT_3=191,18
3436
PRIMER_LEFT_3_TM=57.048
3437
PRIMER_RIGHT_3_TM=62.005
3438
PRIMER_LEFT_3_GC_PERCENT=40.909
3439
PRIMER_RIGHT_3_GC_PERCENT=72.222
3440
PRIMER_LEFT_3_SELF_ANY=4.00
3441
PRIMER_RIGHT_3_SELF_ANY=1.00
3442
PRIMER_LEFT_3_SELF_END=3.00
3443
PRIMER_RIGHT_3_SELF_END=0.00
3444
PRIMER_LEFT_3_END_STABILITY=6.6000
3445
PRIMER_RIGHT_3_END_STABILITY=9.4000
3446
PRIMER_PAIR_3_COMPL_ANY=4.00
3447
PRIMER_PAIR_3_COMPL_END=0.00
3448
PRIMER_PRODUCT_SIZE_3=174
3449
PRIMER_PAIR_PENALTY_4=9.1359
3450
PRIMER_LEFT_4_PENALTY=5.131103
3451
PRIMER_RIGHT_4_PENALTY=4.004782
3452
PRIMER_LEFT_4_SEQUENCE=AGAGAGGTTTCATTTTCTCTGCT
3453
PRIMER_RIGHT_4_SEQUENCE=CTGTCCCTCCCTCCCTCC
3454
PRIMER_LEFT_4=48,23
3455
PRIMER_RIGHT_4=191,18
3456
PRIMER_LEFT_4_TM=57.869
3457
PRIMER_RIGHT_4_TM=62.005
3458
PRIMER_LEFT_4_GC_PERCENT=39.130
3459
PRIMER_RIGHT_4_GC_PERCENT=72.222
3460
PRIMER_LEFT_4_SELF_ANY=5.00
3461
PRIMER_RIGHT_4_SELF_ANY=1.00
3462
PRIMER_LEFT_4_SELF_END=0.00
3463
PRIMER_RIGHT_4_SELF_END=0.00
3464
PRIMER_LEFT_4_END_STABILITY=8.2000
3465
PRIMER_RIGHT_4_END_STABILITY=9.4000
3466
PRIMER_PAIR_4_COMPL_ANY=5.00
3467
PRIMER_PAIR_4_COMPL_END=0.00
3468
PRIMER_PRODUCT_SIZE_4=144
3469
PRIMER_PAIR_PENALTY_5=9.2025
3470
PRIMER_LEFT_5_PENALTY=5.197674
3471
PRIMER_RIGHT_5_PENALTY=4.004782
3472
PRIMER_LEFT_5_SEQUENCE=TGCTAAGTAATGAGTTCAAAGCC
3473
PRIMER_RIGHT_5_SEQUENCE=CTGTCCCTCCCTCCCTCC
3474
PRIMER_LEFT_5=67,23
3475
PRIMER_RIGHT_5=191,18
3476
PRIMER_LEFT_5_TM=57.802
3477
PRIMER_RIGHT_5_TM=62.005
3478
PRIMER_LEFT_5_GC_PERCENT=39.130
3479
PRIMER_RIGHT_5_GC_PERCENT=72.222
3480
PRIMER_LEFT_5_SELF_ANY=4.00
3481
PRIMER_RIGHT_5_SELF_ANY=1.00
3482
PRIMER_LEFT_5_SELF_END=1.00
3483
PRIMER_RIGHT_5_SELF_END=0.00
3484
PRIMER_LEFT_5_END_STABILITY=9.7000
3485
PRIMER_RIGHT_5_END_STABILITY=9.4000
3486
PRIMER_PAIR_5_COMPL_ANY=3.00
3487
PRIMER_PAIR_5_COMPL_END=0.00
3488
PRIMER_PRODUCT_SIZE_5=125
3489
PRIMER_PAIR_PENALTY_6=9.2792
3490
PRIMER_LEFT_6_PENALTY=5.274423
3491
PRIMER_RIGHT_6_PENALTY=4.004782
3492
PRIMER_LEFT_6_SEQUENCE=GACATCCTGTTACCATGTAGGAGTT
3493
PRIMER_RIGHT_6_SEQUENCE=CTGTCCCTCCCTCCCTCC
3494
PRIMER_LEFT_6=22,25
3495
PRIMER_RIGHT_6=191,18
3496
PRIMER_LEFT_6_TM=59.726
3497
PRIMER_RIGHT_6_TM=62.005
3498
PRIMER_LEFT_6_GC_PERCENT=44.000
3499
PRIMER_RIGHT_6_GC_PERCENT=72.222
3500
PRIMER_LEFT_6_SELF_ANY=4.00
3501
PRIMER_RIGHT_6_SELF_ANY=1.00
3502
PRIMER_LEFT_6_SELF_END=0.00
3503
PRIMER_RIGHT_6_SELF_END=0.00
3504
PRIMER_LEFT_6_END_STABILITY=6.4000
3505
PRIMER_RIGHT_6_END_STABILITY=9.4000
3506
PRIMER_PAIR_6_COMPL_ANY=4.00
3507
PRIMER_PAIR_6_COMPL_END=2.00
3508
PRIMER_PRODUCT_SIZE_6=170
3509
PRIMER_PAIR_PENALTY_7=9.2850
3510
PRIMER_LEFT_7_PENALTY=5.280169
3511
PRIMER_RIGHT_7_PENALTY=4.004782
3512
PRIMER_LEFT_7_SEQUENCE=CTGCTAAGTAATGAGTTCAAAGCC
3513
PRIMER_RIGHT_7_SEQUENCE=CTGTCCCTCCCTCCCTCC
3514
PRIMER_LEFT_7=66,24
3515
PRIMER_RIGHT_7=191,18
3516
PRIMER_LEFT_7_TM=58.720
3517
PRIMER_RIGHT_7_TM=62.005
3518
PRIMER_LEFT_7_GC_PERCENT=41.667
3519
PRIMER_RIGHT_7_GC_PERCENT=72.222
3520
PRIMER_LEFT_7_SELF_ANY=4.00
3521
PRIMER_RIGHT_7_SELF_ANY=1.00
3522
PRIMER_LEFT_7_SELF_END=1.00
3523
PRIMER_RIGHT_7_SELF_END=0.00
3524
PRIMER_LEFT_7_END_STABILITY=9.7000
3525
PRIMER_RIGHT_7_END_STABILITY=9.4000
3526
PRIMER_PAIR_7_COMPL_ANY=3.00
3527
PRIMER_PAIR_7_COMPL_END=0.00
3528
PRIMER_PRODUCT_SIZE_7=126
3529
=
3530
MARKER_NAME=09.RHAP34FA9.seq
3531
SEQUENCE=NNNNNNNNNNNNNNNNNNNNTGATCAGGATTTCAGTGCTAATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCCTTTGGTAACTCCTGTTGTACAAGGGCTCCAAAAAGCAGGAAAGAATCAGAAACAGCCCCTGTTCCCTCTGTGAGGAAACCTACAAAAATGCAAGCAACACAAATATAATGTGTATACAGATAGCTTAGGTTAGATTCAAGCAGGCTCTTGTATTTTCAGCTCAGTCTCAGTGTAGCACCTGTAAATGCAGGTTGATTGAGGTTTATTTAT
3532
INCLUDED_REGION=20,280
3533
TARGET=44,42,CA
3534
PRIMER_PAIR_PENALTY=7.2009
3535
PRIMER_LEFT_PENALTY=6.971746
3536
PRIMER_RIGHT_PENALTY=0.229181
3537
PRIMER_LEFT_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
3538
PRIMER_RIGHT_SEQUENCE=TTCCTCACAGAGGGAACAGG
3539
PRIMER_LEFT=20,24
3540
PRIMER_RIGHT=166,20
3541
PRIMER_LEFT_TM=57.028
3542
PRIMER_RIGHT_TM=60.229
3543
PRIMER_LEFT_GC_PERCENT=33.333
3544
PRIMER_RIGHT_GC_PERCENT=55.000
3545
PRIMER_LEFT_SELF_ANY=6.00
3546
PRIMER_RIGHT_SELF_ANY=5.00
3547
PRIMER_LEFT_SELF_END=4.00
3548
PRIMER_RIGHT_SELF_END=0.00
3549
PRIMER_LEFT_END_STABILITY=5.8000
3550
PRIMER_RIGHT_END_STABILITY=7.9000
3551
PRIMER_PAIR_COMPL_ANY=4.00
3552
PRIMER_PAIR_COMPL_END=0.00
3553
PRIMER_PRODUCT_SIZE=147
3554
PRIMER_PAIR_PENALTY_1=7.6879
3555
PRIMER_LEFT_1_PENALTY=6.971746
3556
PRIMER_RIGHT_1_PENALTY=0.716126
3557
PRIMER_LEFT_1_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
3558
PRIMER_RIGHT_1_SEQUENCE=CAGGGGCTGTTTCTGATTCT
3559
PRIMER_LEFT_1=20,24
3560
PRIMER_RIGHT_1=150,20
3561
PRIMER_LEFT_1_TM=57.028
3562
PRIMER_RIGHT_1_TM=59.284
3563
PRIMER_LEFT_1_GC_PERCENT=33.333
3564
PRIMER_RIGHT_1_GC_PERCENT=50.000
3565
PRIMER_LEFT_1_SELF_ANY=6.00
3566
PRIMER_RIGHT_1_SELF_ANY=3.00
3567
PRIMER_LEFT_1_SELF_END=4.00
3568
PRIMER_RIGHT_1_SELF_END=1.00
3569
PRIMER_LEFT_1_END_STABILITY=5.8000
3570
PRIMER_RIGHT_1_END_STABILITY=6.6000
3571
PRIMER_PAIR_1_COMPL_ANY=5.00
3572
PRIMER_PAIR_1_COMPL_END=1.00
3573
PRIMER_PRODUCT_SIZE_1=131
3574
PRIMER_PAIR_PENALTY_2=7.8310
3575
PRIMER_LEFT_2_PENALTY=6.971746
3576
PRIMER_RIGHT_2_PENALTY=0.859212
3577
PRIMER_LEFT_2_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
3578
PRIMER_RIGHT_2_SEQUENCE=ACAGGGGCTGTTTCTGATTC
3579
PRIMER_LEFT_2=20,24
3580
PRIMER_RIGHT_2=151,20
3581
PRIMER_LEFT_2_TM=57.028
3582
PRIMER_RIGHT_2_TM=59.141
3583
PRIMER_LEFT_2_GC_PERCENT=33.333
3584
PRIMER_RIGHT_2_GC_PERCENT=50.000
3585
PRIMER_LEFT_2_SELF_ANY=6.00
3586
PRIMER_RIGHT_2_SELF_ANY=5.00
3587
PRIMER_LEFT_2_SELF_END=4.00
3588
PRIMER_RIGHT_2_SELF_END=3.00
3589
PRIMER_LEFT_2_END_STABILITY=5.8000
3590
PRIMER_RIGHT_2_END_STABILITY=6.6000
3591
PRIMER_PAIR_2_COMPL_ANY=5.00
3592
PRIMER_PAIR_2_COMPL_END=0.00
3593
PRIMER_PRODUCT_SIZE_2=132
3594
PRIMER_PAIR_PENALTY_3=7.8310
3595
PRIMER_LEFT_3_PENALTY=6.971746
3596
PRIMER_RIGHT_3_PENALTY=0.859212
3597
PRIMER_LEFT_3_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
3598
PRIMER_RIGHT_3_SEQUENCE=GAACAGGGGCTGTTTCTGAT
3599
PRIMER_LEFT_3=20,24
3600
PRIMER_RIGHT_3=153,20
3601
PRIMER_LEFT_3_TM=57.028
3602
PRIMER_RIGHT_3_TM=59.141
3603
PRIMER_LEFT_3_GC_PERCENT=33.333
3604
PRIMER_RIGHT_3_GC_PERCENT=50.000
3605
PRIMER_LEFT_3_SELF_ANY=6.00
3606
PRIMER_RIGHT_3_SELF_ANY=7.00
3607
PRIMER_LEFT_3_SELF_END=4.00
3608
PRIMER_RIGHT_3_SELF_END=2.00
3609
PRIMER_LEFT_3_END_STABILITY=5.8000
3610
PRIMER_RIGHT_3_END_STABILITY=6.6000
3611
PRIMER_PAIR_3_COMPL_ANY=5.00
3612
PRIMER_PAIR_3_COMPL_END=2.00
3613
PRIMER_PRODUCT_SIZE_3=134
3614
PRIMER_PAIR_PENALTY_4=8.0391
3615
PRIMER_LEFT_4_PENALTY=6.971746
3616
PRIMER_RIGHT_4_PENALTY=1.067353
3617
PRIMER_LEFT_4_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
3618
PRIMER_RIGHT_4_SEQUENCE=GGGCTGTTTCTGATTCTTTCC
3619
PRIMER_LEFT_4=20,24
3620
PRIMER_RIGHT_4=147,21
3621
PRIMER_LEFT_4_TM=57.028
3622
PRIMER_RIGHT_4_TM=60.067
3623
PRIMER_LEFT_4_GC_PERCENT=33.333
3624
PRIMER_RIGHT_4_GC_PERCENT=47.619
3625
PRIMER_LEFT_4_SELF_ANY=6.00
3626
PRIMER_RIGHT_4_SELF_ANY=3.00
3627
PRIMER_LEFT_4_SELF_END=4.00
3628
PRIMER_RIGHT_4_SELF_END=0.00
3629
PRIMER_LEFT_4_END_STABILITY=5.8000
3630
PRIMER_RIGHT_4_END_STABILITY=8.5000
3631
PRIMER_PAIR_4_COMPL_ANY=5.00
3632
PRIMER_PAIR_4_COMPL_END=0.00
3633
PRIMER_PRODUCT_SIZE_4=128
3634
PRIMER_PAIR_PENALTY_5=8.0391
3635
PRIMER_LEFT_5_PENALTY=6.971746
3636
PRIMER_RIGHT_5_PENALTY=1.067353
3637
PRIMER_LEFT_5_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
3638
PRIMER_RIGHT_5_SEQUENCE=GGGGCTGTTTCTGATTCTTTC
3639
PRIMER_LEFT_5=20,24
3640
PRIMER_RIGHT_5=148,21
3641
PRIMER_LEFT_5_TM=57.028
3642
PRIMER_RIGHT_5_TM=60.067
3643
PRIMER_LEFT_5_GC_PERCENT=33.333
3644
PRIMER_RIGHT_5_GC_PERCENT=47.619
3645
PRIMER_LEFT_5_SELF_ANY=6.00
3646
PRIMER_RIGHT_5_SELF_ANY=3.00
3647
PRIMER_LEFT_5_SELF_END=4.00
3648
PRIMER_RIGHT_5_SELF_END=0.00
3649
PRIMER_LEFT_5_END_STABILITY=5.8000
3650
PRIMER_RIGHT_5_END_STABILITY=7.0000
3651
PRIMER_PAIR_5_COMPL_ANY=5.00
3652
PRIMER_PAIR_5_COMPL_END=0.00
3653
PRIMER_PRODUCT_SIZE_5=129
3654
PRIMER_PAIR_PENALTY_6=8.0963
3655
PRIMER_LEFT_6_PENALTY=6.971746
3656
PRIMER_RIGHT_6_PENALTY=1.124598
3657
PRIMER_LEFT_6_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
3658
PRIMER_RIGHT_6_SEQUENCE=ACAGGGGCTGTTTCTGATTCT
3659
PRIMER_LEFT_6=20,24
3660
PRIMER_RIGHT_6=151,21
3661
PRIMER_LEFT_6_TM=57.028
3662
PRIMER_RIGHT_6_TM=60.125
3663
PRIMER_LEFT_6_GC_PERCENT=33.333
3664
PRIMER_RIGHT_6_GC_PERCENT=47.619
3665
PRIMER_LEFT_6_SELF_ANY=6.00
3666
PRIMER_RIGHT_6_SELF_ANY=5.00
3667
PRIMER_LEFT_6_SELF_END=4.00
3668
PRIMER_RIGHT_6_SELF_END=1.00
3669
PRIMER_LEFT_6_END_STABILITY=5.8000
3670
PRIMER_RIGHT_6_END_STABILITY=6.6000
3671
PRIMER_PAIR_6_COMPL_ANY=5.00
3672
PRIMER_PAIR_6_COMPL_END=1.00
3673
PRIMER_PRODUCT_SIZE_6=132
3674
PRIMER_PAIR_PENALTY_7=8.1993
3675
PRIMER_LEFT_7_PENALTY=6.971746
3676
PRIMER_RIGHT_7_PENALTY=1.227592
3677
PRIMER_LEFT_7_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
3678
PRIMER_RIGHT_7_SEQUENCE=TTTGTGTTGCTTGCATTTTTG
3679
PRIMER_LEFT_7=20,24
3680
PRIMER_RIGHT_7=192,21
3681
PRIMER_LEFT_7_TM=57.028
3682
PRIMER_RIGHT_7_TM=59.772
3683
PRIMER_LEFT_7_GC_PERCENT=33.333
3684
PRIMER_RIGHT_7_GC_PERCENT=33.333
3685
PRIMER_LEFT_7_SELF_ANY=6.00
3686
PRIMER_RIGHT_7_SELF_ANY=4.00
3687
PRIMER_LEFT_7_SELF_END=4.00
3688
PRIMER_RIGHT_7_SELF_END=0.00
3689
PRIMER_LEFT_7_END_STABILITY=5.8000
3690
PRIMER_RIGHT_7_END_STABILITY=7.6000
3691
PRIMER_PAIR_7_COMPL_ANY=3.00
3692
PRIMER_PAIR_7_COMPL_END=3.00
3693
PRIMER_PRODUCT_SIZE_7=173
3694
=
3695
MARKER_NAME=10.RHAP34FA10.seq
3696
SEQUENCE=NNNNNNNNNNNNACACATCTGAGAGTAGGGCAACCCCTGGGGTTCAAAACAGAAAGAAAGAATTCATTATTTAGGATAGAAAGGCAAGTAAATATAGATATCCTGCAATGGGATGAATACAAAGATTTATTTATTTATATTTATATTCATTAATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATCAGAGGACAGGTTGTAGGAGCCAGTTCAGTTCTGTCCTTGCATACCTACATTCTGGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
3697
INCLUDED_REGION=12,236
3698
TARGET=155,32,TG
3699
PRIMER_PAIR_PENALTY=1.7506
3700
PRIMER_LEFT_PENALTY=0.649665
3701
PRIMER_RIGHT_PENALTY=1.100969
3702
PRIMER_LEFT_SEQUENCE=CCTGCAATGGGATGAATACA
3703
PRIMER_RIGHT_SEQUENCE=CAAGGACAGAACTGAACTGGC
3704
PRIMER_LEFT=101,20
3705
PRIMER_RIGHT=229,21
3706
PRIMER_LEFT_TM=59.350
3707
PRIMER_RIGHT_TM=59.899
3708
PRIMER_LEFT_GC_PERCENT=45.000
3709
PRIMER_RIGHT_GC_PERCENT=52.381
3710
PRIMER_LEFT_SELF_ANY=4.00
3711
PRIMER_RIGHT_SELF_ANY=4.00
3712
PRIMER_LEFT_SELF_END=4.00
3713
PRIMER_RIGHT_SELF_END=3.00
3714
PRIMER_LEFT_END_STABILITY=5.6000
3715
PRIMER_RIGHT_END_STABILITY=9.7000
3716
PRIMER_PAIR_COMPL_ANY=3.00
3717
PRIMER_PAIR_COMPL_END=0.00
3718
PRIMER_PRODUCT_SIZE=129
3719
PRIMER_PAIR_PENALTY_1=1.7506
3720
PRIMER_LEFT_1_PENALTY=0.649665
3721
PRIMER_RIGHT_1_PENALTY=1.100969
3722
PRIMER_LEFT_1_SEQUENCE=CCTGCAATGGGATGAATACA
3723
PRIMER_RIGHT_1_SEQUENCE=GCAAGGACAGAACTGAACTGG
3724
PRIMER_LEFT_1=101,20
3725
PRIMER_RIGHT_1=230,21
3726
PRIMER_LEFT_1_TM=59.350
3727
PRIMER_RIGHT_1_TM=59.899
3728
PRIMER_LEFT_1_GC_PERCENT=45.000
3729
PRIMER_RIGHT_1_GC_PERCENT=52.381
3730
PRIMER_LEFT_1_SELF_ANY=4.00
3731
PRIMER_RIGHT_1_SELF_ANY=4.00
3732
PRIMER_LEFT_1_SELF_END=4.00
3733
PRIMER_RIGHT_1_SELF_END=4.00
3734
PRIMER_LEFT_1_END_STABILITY=5.6000
3735
PRIMER_RIGHT_1_END_STABILITY=7.9000
3736
PRIMER_PAIR_1_COMPL_ANY=3.00
3737
PRIMER_PAIR_1_COMPL_END=1.00
3738
PRIMER_PRODUCT_SIZE_1=130
3739
PRIMER_PAIR_PENALTY_2=2.3642
3740
PRIMER_LEFT_2_PENALTY=1.263200
3741
PRIMER_RIGHT_2_PENALTY=1.100969
3742
PRIMER_LEFT_2_SEQUENCE=GATATCCTGCAATGGGATGAA
3743
PRIMER_RIGHT_2_SEQUENCE=CAAGGACAGAACTGAACTGGC
3744
PRIMER_LEFT_2=96,21
3745
PRIMER_RIGHT_2=229,21
3746
PRIMER_LEFT_2_TM=59.737
3747
PRIMER_RIGHT_2_TM=59.899
3748
PRIMER_LEFT_2_GC_PERCENT=42.857
3749
PRIMER_RIGHT_2_GC_PERCENT=52.381
3750
PRIMER_LEFT_2_SELF_ANY=6.00
3751
PRIMER_RIGHT_2_SELF_ANY=4.00
3752
PRIMER_LEFT_2_SELF_END=2.00
3753
PRIMER_RIGHT_2_SELF_END=3.00
3754
PRIMER_LEFT_2_END_STABILITY=6.9000
3755
PRIMER_RIGHT_2_END_STABILITY=9.7000
3756
PRIMER_PAIR_2_COMPL_ANY=4.00
3757
PRIMER_PAIR_2_COMPL_END=0.00
3758
PRIMER_PRODUCT_SIZE_2=134
3759
PRIMER_PAIR_PENALTY_3=2.3642
3760
PRIMER_LEFT_3_PENALTY=1.263200
3761
PRIMER_RIGHT_3_PENALTY=1.100969
3762
PRIMER_LEFT_3_SEQUENCE=GATATCCTGCAATGGGATGAA
3763
PRIMER_RIGHT_3_SEQUENCE=GCAAGGACAGAACTGAACTGG
3764
PRIMER_LEFT_3=96,21
3765
PRIMER_RIGHT_3=230,21
3766
PRIMER_LEFT_3_TM=59.737
3767
PRIMER_RIGHT_3_TM=59.899
3768
PRIMER_LEFT_3_GC_PERCENT=42.857
3769
PRIMER_RIGHT_3_GC_PERCENT=52.381
3770
PRIMER_LEFT_3_SELF_ANY=6.00
3771
PRIMER_RIGHT_3_SELF_ANY=4.00
3772
PRIMER_LEFT_3_SELF_END=2.00
3773
PRIMER_RIGHT_3_SELF_END=4.00
3774
PRIMER_LEFT_3_END_STABILITY=6.9000
3775
PRIMER_RIGHT_3_END_STABILITY=7.9000
3776
PRIMER_PAIR_3_COMPL_ANY=4.00
3777
PRIMER_PAIR_3_COMPL_END=0.00
3778
PRIMER_PRODUCT_SIZE_3=135
3779
PRIMER_PAIR_PENALTY_4=2.5784
3780
PRIMER_LEFT_4_PENALTY=0.649665
3781
PRIMER_RIGHT_4_PENALTY=1.928763
3782
PRIMER_LEFT_4_SEQUENCE=CCTGCAATGGGATGAATACA
3783
PRIMER_RIGHT_4_SEQUENCE=TGCAAGGACAGAACTGAACTG
3784
PRIMER_LEFT_4=101,20
3785
PRIMER_RIGHT_4=231,21
3786
PRIMER_LEFT_4_TM=59.350
3787
PRIMER_RIGHT_4_TM=59.071
3788
PRIMER_LEFT_4_GC_PERCENT=45.000
3789
PRIMER_RIGHT_4_GC_PERCENT=47.619
3790
PRIMER_LEFT_4_SELF_ANY=4.00
3791
PRIMER_RIGHT_4_SELF_ANY=4.00
3792
PRIMER_LEFT_4_SELF_END=4.00
3793
PRIMER_RIGHT_4_SELF_END=3.00
3794
PRIMER_LEFT_4_END_STABILITY=5.6000
3795
PRIMER_RIGHT_4_END_STABILITY=6.7000
3796
PRIMER_PAIR_4_COMPL_ANY=4.00
3797
PRIMER_PAIR_4_COMPL_END=2.00
3798
PRIMER_PRODUCT_SIZE_4=131
3799
PRIMER_PAIR_PENALTY_5=2.6739
3800
PRIMER_LEFT_5_PENALTY=1.427458
3801
PRIMER_RIGHT_5_PENALTY=1.246400
3802
PRIMER_LEFT_5_SEQUENCE=TGGGGTTCAAAACAGAAAGAA
3803
PRIMER_RIGHT_5_SEQUENCE=GGCTCCTACAACCTGTCCTCT
3804
PRIMER_LEFT_5=37,21
3805
PRIMER_RIGHT_5=211,21
3806
PRIMER_LEFT_5_TM=59.573
3807
PRIMER_RIGHT_5_TM=59.754
3808
PRIMER_LEFT_5_GC_PERCENT=38.095
3809
PRIMER_RIGHT_5_GC_PERCENT=57.143
3810
PRIMER_LEFT_5_SELF_ANY=3.00
3811
PRIMER_RIGHT_5_SELF_ANY=3.00
3812
PRIMER_LEFT_5_SELF_END=0.00
3813
PRIMER_RIGHT_5_SELF_END=0.00
3814
PRIMER_LEFT_5_END_STABILITY=7.0000
3815
PRIMER_RIGHT_5_END_STABILITY=7.9000
3816
PRIMER_PAIR_5_COMPL_ANY=4.00
3817
PRIMER_PAIR_5_COMPL_END=2.00
3818
PRIMER_PRODUCT_SIZE_5=175
3819
PRIMER_PAIR_PENALTY_6=2.6834
3820
PRIMER_LEFT_6_PENALTY=1.427458
3821
PRIMER_RIGHT_6_PENALTY=1.255934
3822
PRIMER_LEFT_6_SEQUENCE=TGGGGTTCAAAACAGAAAGAA
3823
PRIMER_RIGHT_6_SEQUENCE=GGCTCCTACAACCTGTCCTC
3824
PRIMER_LEFT_6=37,21
3825
PRIMER_RIGHT_6=211,20
3826
PRIMER_LEFT_6_TM=59.573
3827
PRIMER_RIGHT_6_TM=58.744
3828
PRIMER_LEFT_6_GC_PERCENT=38.095
3829
PRIMER_RIGHT_6_GC_PERCENT=60.000
3830
PRIMER_LEFT_6_SELF_ANY=3.00
3831
PRIMER_RIGHT_6_SELF_ANY=3.00
3832
PRIMER_LEFT_6_SELF_END=0.00
3833
PRIMER_RIGHT_6_SELF_END=0.00
3834
PRIMER_LEFT_6_END_STABILITY=7.0000
3835
PRIMER_RIGHT_6_END_STABILITY=7.9000
3836
PRIMER_PAIR_6_COMPL_ANY=4.00
3837
PRIMER_PAIR_6_COMPL_END=1.00
3838
PRIMER_PRODUCT_SIZE_6=175
3839
PRIMER_PAIR_PENALTY_7=2.7270
3840
PRIMER_LEFT_7_PENALTY=0.649665
3841
PRIMER_RIGHT_7_PENALTY=2.077340
3842
PRIMER_LEFT_7_SEQUENCE=CCTGCAATGGGATGAATACA
3843
PRIMER_RIGHT_7_SEQUENCE=AAGGACAGAACTGAACTGGCTC
3844
PRIMER_LEFT_7=101,20
3845
PRIMER_RIGHT_7=228,22
3846
PRIMER_LEFT_7_TM=59.350
3847
PRIMER_RIGHT_7_TM=59.923
3848
PRIMER_LEFT_7_GC_PERCENT=45.000
3849
PRIMER_RIGHT_7_GC_PERCENT=50.000
3850
PRIMER_LEFT_7_SELF_ANY=4.00
3851
PRIMER_RIGHT_7_SELF_ANY=4.00
3852
PRIMER_LEFT_7_SELF_END=4.00
3853
PRIMER_RIGHT_7_SELF_END=2.00
3854
PRIMER_LEFT_7_END_STABILITY=5.6000
3855
PRIMER_RIGHT_7_END_STABILITY=9.4000
3856
PRIMER_PAIR_7_COMPL_ANY=3.00
3857
PRIMER_PAIR_7_COMPL_END=0.00
3858
PRIMER_PRODUCT_SIZE_7=128
3859
=
3860
MARKER_NAME=13.RHAP34FB1.seq
3861
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNGAGAGGTGTATATAGAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGTATCCAAAACTCTTTAGTCTTAATGTATTTATATACCTTTATTGTTATAAAATTCCTGTTTAAAACACAGTCTGAACAAGGTGACTTCTTTTAGAATTTGTCAGTAAGGAACACATTTTTTCTTTACCTATGTCTTTTTCTTAGACTGGGTCAGCACAATATTTGTAACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
3862
INCLUDED_REGION=21,244
3863
TARGET=22,16,TA
3864
TARGET=57,34,GT
3865
=
3866
MARKER_NAME=14.RHAP34FB2.seq
3867
SEQUENCE=NNNNNNNNNNNNNACTCATATACATAAAATAAATAGATATTTTTAAATAAACAGAAGATTGCAACGAAATCAAGCAGAATTCAAGACAATGAATAAATAACAAAATCTAAAAGGCTCAAGACTGCAACACTCCTGTGGGAGCACACATGTGTGCATGGAACACNCAAATGCGCNCNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTNCACACAANNCTACNGCNNCACNNTTAGCACNCGCACAAGNNNNNNNNNNNNNNTTCNGCNACNACATNNNNANNNCNCNAANACNNCNACNNCNCNNTNNC
3868
INCLUDED_REGION=13,303
3869
TARGET=177,33,AC
3870
TARGET=254,14,AC
3871
=
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