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- Committer: Bazaar Package Importer
- Author(s): Charles Plessy
- Date: 2006-09-28 20:18:54 UTC
- Revision ID: james.westby@ubuntu.com-20060928201854-45pwapz5e3a6d684
Tags: upstream-1.0b
Import upstream version 1.0b
- files added:
- src
- test
- test/primer1_list_last
- test/primer1_list_tmp
- test/primer_global_err
- test/primer_list_last
- test/primer_list_tmp
added
removed
test/primer_rat_output
1
PRIMER_OPT_SIZE=20
2
PRIMER_MAX_POLY_X=100
3
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
4
PRIMER_PRODUCT_SIZE_RANGE=125-175 75-1000
5
PRIMER_MAX_GC=80.0
6
PRIMER_MAX_SIZE=25
7
PRIMER_MAX_TM=63.0
8
PRIMER_MIN_SIZE=18
9
PRIMER_MIN_GC=20
10
PRIMER_MIN_TM=57.0
11
PRIMER_OPT_TM=60.0
12
PRIMER_SELF_ANY=12
13
PRIMER_SELF_END=6
14
MARKER_NAME=01.RHAP33FA1.seq
15
SEQUENCE=NNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGGAAACAGTATAAATAAGCAAAGATATTTGAATCCTGTCACCTTTTCATCCTCTGTCCTTTTGCCCAAGATAACATTGTTATAGTTGCCTTTACCCTGCACTGTACAACTCCTACTAAACTTCTTAGGTAGGAGGTGTGTTGGCTTTTCCTTGNGGGNTGGGTAGTCACAAAGTGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAANTAATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
16
INCLUDED_REGION=20,315
17
TARGET=81,30,CA
18
PRIMER_COMMENT=CL is GCCTTCATTAGAAAGTGAAAGTAAA
19
PRIMER_COMMENT=CR is GGCAAAAGGACAGAGGATGA
20
PRIMER_PAIR_PENALTY=7.7339
21
PRIMER_LEFT_PENALTY=7.538496
22
PRIMER_RIGHT_PENALTY=0.195358
23
PRIMER_LEFT_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA
24
PRIMER_RIGHT_SEQUENCE=GGCAAAAGGACAGAGGATGA
25
PRIMER_LEFT=20,25
26
PRIMER_RIGHT=177,20
27
PRIMER_LEFT_TM=57.462
28
PRIMER_RIGHT_TM=60.195
29
PRIMER_LEFT_GC_PERCENT=32.000
30
PRIMER_RIGHT_GC_PERCENT=50.000
31
PRIMER_LEFT_SELF_ANY=5.00
32
PRIMER_RIGHT_SELF_ANY=2.00
33
PRIMER_LEFT_SELF_END=0.00
34
PRIMER_RIGHT_SELF_END=1.00
35
PRIMER_LEFT_END_STABILITY=6.0000
36
PRIMER_RIGHT_END_STABILITY=6.6000
37
PRIMER_PAIR_COMPL_ANY=4.00
38
PRIMER_PAIR_COMPL_END=0.00
39
PRIMER_PRODUCT_SIZE=158
40
PRIMER_PAIR_PENALTY_1=7.8680
41
PRIMER_LEFT_1_PENALTY=7.538496
42
PRIMER_RIGHT_1_PENALTY=0.329550
43
PRIMER_LEFT_1_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA
44
PRIMER_RIGHT_1_SEQUENCE=ATCTTGGGCAAAAGGACAGA
45
PRIMER_LEFT_1=20,25
46
PRIMER_RIGHT_1=183,20
47
PRIMER_LEFT_1_TM=57.462
48
PRIMER_RIGHT_1_TM=59.670
49
PRIMER_LEFT_1_GC_PERCENT=32.000
50
PRIMER_RIGHT_1_GC_PERCENT=45.000
51
PRIMER_LEFT_1_SELF_ANY=5.00
52
PRIMER_RIGHT_1_SELF_ANY=4.00
53
PRIMER_LEFT_1_SELF_END=0.00
54
PRIMER_RIGHT_1_SELF_END=0.00
55
PRIMER_LEFT_1_END_STABILITY=6.0000
56
PRIMER_RIGHT_1_END_STABILITY=6.4000
57
PRIMER_PAIR_1_COMPL_ANY=4.00
58
PRIMER_PAIR_1_COMPL_END=0.00
59
PRIMER_PRODUCT_SIZE_1=164
60
PRIMER_PAIR_PENALTY_2=7.9049
61
PRIMER_LEFT_2_PENALTY=7.538496
62
PRIMER_RIGHT_2_PENALTY=0.366450
63
PRIMER_LEFT_2_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA
64
PRIMER_RIGHT_2_SEQUENCE=TCTTGGGCAAAAGGACAGAG
65
PRIMER_LEFT_2=20,25
66
PRIMER_RIGHT_2=182,20
67
PRIMER_LEFT_2_TM=57.462
68
PRIMER_RIGHT_2_TM=60.366
69
PRIMER_LEFT_2_GC_PERCENT=32.000
70
PRIMER_RIGHT_2_GC_PERCENT=50.000
71
PRIMER_LEFT_2_SELF_ANY=5.00
72
PRIMER_RIGHT_2_SELF_ANY=4.00
73
PRIMER_LEFT_2_SELF_END=0.00
74
PRIMER_RIGHT_2_SELF_END=0.00
75
PRIMER_LEFT_2_END_STABILITY=6.0000
76
PRIMER_RIGHT_2_END_STABILITY=6.7000
77
PRIMER_PAIR_2_COMPL_ANY=4.00
78
PRIMER_PAIR_2_COMPL_END=0.00
79
PRIMER_PRODUCT_SIZE_2=163
80
PRIMER_PAIR_PENALTY_3=8.8450
81
PRIMER_LEFT_3_PENALTY=7.538496
82
PRIMER_RIGHT_3_PENALTY=1.306521
83
PRIMER_LEFT_3_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA
84
PRIMER_RIGHT_3_SEQUENCE=TATCTTGGGCAAAAGGACAGA
85
PRIMER_LEFT_3=20,25
86
PRIMER_RIGHT_3=184,21
87
PRIMER_LEFT_3_TM=57.462
88
PRIMER_RIGHT_3_TM=59.693
89
PRIMER_LEFT_3_GC_PERCENT=32.000
90
PRIMER_RIGHT_3_GC_PERCENT=42.857
91
PRIMER_LEFT_3_SELF_ANY=5.00
92
PRIMER_RIGHT_3_SELF_ANY=4.00
93
PRIMER_LEFT_3_SELF_END=0.00
94
PRIMER_RIGHT_3_SELF_END=0.00
95
PRIMER_LEFT_3_END_STABILITY=6.0000
96
PRIMER_RIGHT_3_END_STABILITY=6.4000
97
PRIMER_PAIR_3_COMPL_ANY=4.00
98
PRIMER_PAIR_3_COMPL_END=0.00
99
PRIMER_PRODUCT_SIZE_3=165
100
PRIMER_PAIR_PENALTY_4=9.1539
101
PRIMER_LEFT_4_PENALTY=7.538496
102
PRIMER_RIGHT_4_PENALTY=1.615394
103
PRIMER_LEFT_4_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA
104
PRIMER_RIGHT_4_SEQUENCE=ATCTTGGGCAAAAGGACAGAG
105
PRIMER_LEFT_4=20,25
106
PRIMER_RIGHT_4=183,21
107
PRIMER_LEFT_4_TM=57.462
108
PRIMER_RIGHT_4_TM=60.615
109
PRIMER_LEFT_4_GC_PERCENT=32.000
110
PRIMER_RIGHT_4_GC_PERCENT=47.619
111
PRIMER_LEFT_4_SELF_ANY=5.00
112
PRIMER_RIGHT_4_SELF_ANY=4.00
113
PRIMER_LEFT_4_SELF_END=0.00
114
PRIMER_RIGHT_4_SELF_END=0.00
115
PRIMER_LEFT_4_END_STABILITY=6.0000
116
PRIMER_RIGHT_4_END_STABILITY=6.7000
117
PRIMER_PAIR_4_COMPL_ANY=4.00
118
PRIMER_PAIR_4_COMPL_END=0.00
119
PRIMER_PRODUCT_SIZE_4=164
120
=
121
MARKER_NAME=01.RHAP34FA1.seq
122
SEQUENCE=NNNNNNNNNNNNNNNNNNNACTTGTCATTTCTCAGAGACATCTATACCTCAGAGACAAACTTCTCAGAAAACAACCCTAAGGTTGAACAAAACATGGCTGTATGTATCTATATTATTACACGCGCGTGGGNNANNNNNNNNNNNNNNNNCGTTGATTTTTTATACTAACAAGANNATGTCCATATAACATGCTAGTATATCATACACACATACNNNNNNNNNNNNNNNNTTATACNNNNNNNNNNNNNNNNAGGAATGTGTGTGCNACCTNATGNTTAACNNCAAGTGTACCCNNGAATNTGTNGNTGNTGGCNT
123
INCLUDED_REGION=19,296
124
TARGET=134,15,CA
125
TARGET=213,16,AT
126
TARGET=235,16,AT
127
PRIMER_COMMENT=CL is
128
PRIMER_COMMENT=CR is
129
=
130
MARKER_NAME=03.RHAP34FA3.seq
131
SEQUENCE=TNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGCAGGAGGATGAAATGCAATCTGAAGAGAGCCCCTGAGCCAGTCCAGCCTCCCCATCATGGCCTTTGCCTTCCAACCCCTGCGTTCTTCCGGGCCTCTGTGAAATGCTATCTTGATAATGTCTGTAATCACTTGATTGCAGCCAAGCTAAAGGCTGGCCCTGACTGGGATGCAGAATGAAGAGTTGTGGGGAGGCGAGGGATAGCAGGAACGAACCCAAAAAACACACTGCCCTTTCACACAGTCAT
132
INCLUDED_REGION=0,278
133
TARGET=1,28,CA
134
PRIMER_COMMENT=CL is
135
PRIMER_COMMENT=CR is
136
=
137
MARKER_NAME=03.RHAP34FE3.seq
138
SEQUENCE=NNNNNNNNNNNNNNNNNTCATACAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCATATTATGTATATGCTGCATCTAACTTTATCCTTTCAGACAGCCTAGCTGCAGCTCAGGATGCTGTCTATTCCAGACTTACCTCAAACATGCAGCAGCAGCAGTACATGAAGAAATTGAATTATTCCATGTTAAGGCAAAGATAAACACTATCCACAAATATAGGCTTATTAGCNAGGTCTAAAGAAAAATTTCACTCACTAAGCACTCAA
139
INCLUDED_REGION=17,284
140
TARGET=27,60,AC
141
PRIMER_COMMENT=CL is
142
PRIMER_COMMENT=CR is
143
=
144
MARKER_NAME=04.RHAP33FE4.seq
145
SEQUENCE=NNNNNNNNNNNNNNNNNNTCCCAGAATGCAATGCCCAGAAAAGTCTCGCCCTCAATCTCATAGTTTCACTCAGCGTCTAGCACCGAGGAAAGTTTTGTGAGAAGGGAGGAGTAAGTACTGGAGAGAGAAGAGGAATTAAACAATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTGTGNNNNNNNNNNNNNNNNNNNNNNTGTGTGTGTGTATAGGTATACCGNATATGTGTGTGGTGTATAGGCATAGGTATATGTATATGTGCNNNNNNNNNNNNNGNNNNNNNNNNNNNNNCAGANAGAGAGACNANACCNCNCNNGGNGNNNCNNNNGNGCNNAGAGNGNGNCNGANNGNGAGNGACCAC
146
INCLUDED_REGION=18,367
147
TARGET=146,46,AT
148
TARGET=199,22,TA
149
TARGET=286,13,GT
150
TARGET=300,15,AG
151
PRIMER_COMMENT=CL is TGAGAAGGGAGGAGTAAGTACTGG
152
PRIMER_COMMENT=CR is CCTATGCCTATACACCACACACA
153
PRIMER_PAIR_PENALTY=7.3535
154
PRIMER_LEFT_PENALTY=4.174047
155
PRIMER_RIGHT_PENALTY=3.179421
156
PRIMER_LEFT_SEQUENCE=TGAGAAGGGAGGAGTAAGTACTGG
157
PRIMER_RIGHT_SEQUENCE=CCTATGCCTATACACCACACACA
158
PRIMER_LEFT=97,24
159
PRIMER_RIGHT=270,23
160
PRIMER_LEFT_TM=60.174
161
PRIMER_RIGHT_TM=59.821
162
PRIMER_LEFT_GC_PERCENT=50.000
163
PRIMER_RIGHT_GC_PERCENT=47.826
164
PRIMER_LEFT_SELF_ANY=6.00
165
PRIMER_RIGHT_SELF_ANY=4.00
166
PRIMER_LEFT_SELF_END=2.00
167
PRIMER_RIGHT_SELF_END=0.00
168
PRIMER_LEFT_END_STABILITY=7.9000
169
PRIMER_RIGHT_END_STABILITY=6.4000
170
PRIMER_PAIR_COMPL_ANY=4.00
171
PRIMER_PAIR_COMPL_END=1.00
172
PRIMER_PRODUCT_SIZE=174
173
PRIMER_PAIR_PENALTY_1=7.8443
174
PRIMER_LEFT_1_PENALTY=4.664847
175
PRIMER_RIGHT_1_PENALTY=3.179421
176
PRIMER_LEFT_1_SEQUENCE=GAAGGGAGGAGTAAGTACTGGAGA
177
PRIMER_RIGHT_1_SEQUENCE=CCTATGCCTATACACCACACACA
178
PRIMER_LEFT_1=100,24
179
PRIMER_RIGHT_1=270,23
180
PRIMER_LEFT_1_TM=59.335
181
PRIMER_RIGHT_1_TM=59.821
182
PRIMER_LEFT_1_GC_PERCENT=50.000
183
PRIMER_RIGHT_1_GC_PERCENT=47.826
184
PRIMER_LEFT_1_SELF_ANY=6.00
185
PRIMER_RIGHT_1_SELF_ANY=4.00
186
PRIMER_LEFT_1_SELF_END=0.00
187
PRIMER_RIGHT_1_SELF_END=0.00
188
PRIMER_LEFT_1_END_STABILITY=7.9000
189
PRIMER_RIGHT_1_END_STABILITY=6.4000
190
PRIMER_PAIR_1_COMPL_ANY=4.00
191
PRIMER_PAIR_1_COMPL_END=1.00
192
PRIMER_PRODUCT_SIZE_1=171
193
PRIMER_PAIR_PENALTY_2=7.8443
194
PRIMER_LEFT_2_PENALTY=4.664847
195
PRIMER_RIGHT_2_PENALTY=3.179421
196
PRIMER_LEFT_2_SEQUENCE=GAGAAGGGAGGAGTAAGTACTGGA
197
PRIMER_RIGHT_2_SEQUENCE=CCTATGCCTATACACCACACACA
198
PRIMER_LEFT_2=98,24
199
PRIMER_RIGHT_2=270,23
200
PRIMER_LEFT_2_TM=59.335
201
PRIMER_RIGHT_2_TM=59.821
202
PRIMER_LEFT_2_GC_PERCENT=50.000
203
PRIMER_RIGHT_2_GC_PERCENT=47.826
204
PRIMER_LEFT_2_SELF_ANY=6.00
205
PRIMER_RIGHT_2_SELF_ANY=4.00
206
PRIMER_LEFT_2_SELF_END=2.00
207
PRIMER_RIGHT_2_SELF_END=0.00
208
PRIMER_LEFT_2_END_STABILITY=8.2000
209
PRIMER_RIGHT_2_END_STABILITY=6.4000
210
PRIMER_PAIR_2_COMPL_ANY=4.00
211
PRIMER_PAIR_2_COMPL_END=1.00
212
PRIMER_PRODUCT_SIZE_2=173
213
PRIMER_PAIR_PENALTY_3=8.1299
214
PRIMER_LEFT_3_PENALTY=4.950517
215
PRIMER_RIGHT_3_PENALTY=3.179421
216
PRIMER_LEFT_3_SEQUENCE=GGGAGGAGTAAGTACTGGAGAGA
217
PRIMER_RIGHT_3_SEQUENCE=CCTATGCCTATACACCACACACA
218
PRIMER_LEFT_3=103,23
219
PRIMER_RIGHT_3=270,23
220
PRIMER_LEFT_3_TM=58.049
221
PRIMER_RIGHT_3_TM=59.821
222
PRIMER_LEFT_3_GC_PERCENT=52.174
223
PRIMER_RIGHT_3_GC_PERCENT=47.826
224
PRIMER_LEFT_3_SELF_ANY=6.00
225
PRIMER_RIGHT_3_SELF_ANY=4.00
226
PRIMER_LEFT_3_SELF_END=0.00
227
PRIMER_RIGHT_3_SELF_END=0.00
228
PRIMER_LEFT_3_END_STABILITY=6.4000
229
PRIMER_RIGHT_3_END_STABILITY=6.4000
230
PRIMER_PAIR_3_COMPL_ANY=4.00
231
PRIMER_PAIR_3_COMPL_END=1.00
232
PRIMER_PRODUCT_SIZE_3=168
233
PRIMER_PAIR_PENALTY_4=8.1856
234
PRIMER_LEFT_4_PENALTY=5.006190
235
PRIMER_RIGHT_4_PENALTY=3.179421
236
PRIMER_LEFT_4_SEQUENCE=GGGAGGAGTAAGTACTGGAGAGAG
237
PRIMER_RIGHT_4_SEQUENCE=CCTATGCCTATACACCACACACA
238
PRIMER_LEFT_4=103,24
239
PRIMER_RIGHT_4=270,23
240
PRIMER_LEFT_4_TM=58.994
241
PRIMER_RIGHT_4_TM=59.821
242
PRIMER_LEFT_4_GC_PERCENT=54.167
243
PRIMER_RIGHT_4_GC_PERCENT=47.826
244
PRIMER_LEFT_4_SELF_ANY=6.00
245
PRIMER_RIGHT_4_SELF_ANY=4.00
246
PRIMER_LEFT_4_SELF_END=0.00
247
PRIMER_RIGHT_4_SELF_END=0.00
248
PRIMER_LEFT_4_END_STABILITY=6.4000
249
PRIMER_RIGHT_4_END_STABILITY=6.4000
250
PRIMER_PAIR_4_COMPL_ANY=4.00
251
PRIMER_PAIR_4_COMPL_END=1.00
252
PRIMER_PRODUCT_SIZE_4=168
253
=
254
MARKER_NAME=05.RHAP33FA5.seq
255
SEQUENCE=NNNNNNNNNNCCTAATACTGTGAAGACAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGGAGGTGTTCCAATAGAGAAGCAAGATCATGACACCCTCTGACCTTTGCCCCTGGCAACCATTTTGGGTATGTTCTCATGGCTCCCTCCACCCCCAGCCTCAGTTTCCCTGAGAGGGAAGGGGGATATAGGGAGAGGAGAGCCGNGCCCCGCCCCCATCCGGGATCANTGTTGGGGGAAGAATGNGTGCAAGCCAGGCCACCNGGGGTTAANGCGGTGACCTCAGCCGNGCGCCCCAGGAGGGGTNNNAGANCCTCNANTTCGTANTCNNTGAGATCAATAANAATGTTTCAATGTGTGAAA
256
INCLUDED_REGION=10,369
257
TARGET=30,44,GT
258
PRIMER_COMMENT=CL is
259
PRIMER_COMMENT=CR is
260
=
261
MARKER_NAME=05.RHAP33FE5.seq
262
SEQUENCE=NNNNNNNNNNNNNNCTTCAGTAANAGATTCTTATCACAGGAAAGAAGGTGGAGAATGATAAACAAGGAGTTCAACATCAACCTCGGGCCTGTATATGTATGAAGTACGTACACCCAAATCCTATACATACATGTTCGTGCAAACACTCAAATACTACATATACTTGTATATACAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTATACAAACACTTATTTATTGGTTTTATTTTGCCTTAAAGGATTAAGTTGTGATTACTAATACATTTTATTTTGCTTTTGGAGACAGGGTCTTGCTAGGTATCCTAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
263
INCLUDED_REGION=14,306
264
TARGET=176,34,CA
265
PRIMER_COMMENT=CL is CATGTTCGTGCAAACACTCA
266
PRIMER_COMMENT=CR is AGACCCTGTCTCCAAAAGCA
267
PRIMER_PAIR_PENALTY=0.8585
268
PRIMER_LEFT_PENALTY=0.702456
269
PRIMER_RIGHT_PENALTY=0.156061
270
PRIMER_LEFT_SEQUENCE=CATGTTCGTGCAAACACTCA
271
PRIMER_RIGHT_SEQUENCE=AGACCCTGTCTCCAAAAGCA
272
PRIMER_LEFT=129,20
273
PRIMER_RIGHT=302,20
274
PRIMER_LEFT_TM=59.298
275
PRIMER_RIGHT_TM=59.844
276
PRIMER_LEFT_GC_PERCENT=45.000
277
PRIMER_RIGHT_GC_PERCENT=50.000
278
PRIMER_LEFT_SELF_ANY=6.00
279
PRIMER_RIGHT_SELF_ANY=5.00
280
PRIMER_LEFT_SELF_END=1.00
281
PRIMER_RIGHT_SELF_END=0.00
282
PRIMER_LEFT_END_STABILITY=6.4000
283
PRIMER_RIGHT_END_STABILITY=8.5000
284
PRIMER_PAIR_COMPL_ANY=4.00
285
PRIMER_PAIR_COMPL_END=0.00
286
PRIMER_PRODUCT_SIZE=174
287
PRIMER_PAIR_PENALTY_1=0.9220
288
PRIMER_LEFT_1_PENALTY=0.702456
289
PRIMER_RIGHT_1_PENALTY=0.219551
290
PRIMER_LEFT_1_SEQUENCE=CATGTTCGTGCAAACACTCA
291
PRIMER_RIGHT_1_SEQUENCE=CCCTGTCTCCAAAAGCAAAA
292
PRIMER_LEFT_1=129,20
293
PRIMER_RIGHT_1=299,20
294
PRIMER_LEFT_1_TM=59.298
295
PRIMER_RIGHT_1_TM=60.220
296
PRIMER_LEFT_1_GC_PERCENT=45.000
297
PRIMER_RIGHT_1_GC_PERCENT=45.000
298
PRIMER_LEFT_1_SELF_ANY=6.00
299
PRIMER_RIGHT_1_SELF_ANY=2.00
300
PRIMER_LEFT_1_SELF_END=1.00
301
PRIMER_RIGHT_1_SELF_END=0.00
302
PRIMER_LEFT_1_END_STABILITY=6.4000
303
PRIMER_RIGHT_1_END_STABILITY=7.6000
304
PRIMER_PAIR_1_COMPL_ANY=4.00
305
PRIMER_PAIR_1_COMPL_END=0.00
306
PRIMER_PRODUCT_SIZE_1=171
307
PRIMER_PAIR_PENALTY_2=0.9348
308
PRIMER_LEFT_2_PENALTY=0.702456
309
PRIMER_RIGHT_2_PENALTY=0.232299
310
PRIMER_LEFT_2_SEQUENCE=CATGTTCGTGCAAACACTCA
311
PRIMER_RIGHT_2_SEQUENCE=GACCCTGTCTCCAAAAGCAA
312
PRIMER_LEFT_2=129,20
313
PRIMER_RIGHT_2=301,20
314
PRIMER_LEFT_2_TM=59.298
315
PRIMER_RIGHT_2_TM=60.232
316
PRIMER_LEFT_2_GC_PERCENT=45.000
317
PRIMER_RIGHT_2_GC_PERCENT=50.000
318
PRIMER_LEFT_2_SELF_ANY=6.00
319
PRIMER_RIGHT_2_SELF_ANY=3.00
320
PRIMER_LEFT_2_SELF_END=1.00
321
PRIMER_RIGHT_2_SELF_END=0.00
322
PRIMER_LEFT_2_END_STABILITY=6.4000
323
PRIMER_RIGHT_2_END_STABILITY=8.5000
324
PRIMER_PAIR_2_COMPL_ANY=4.00
325
PRIMER_PAIR_2_COMPL_END=0.00
326
PRIMER_PRODUCT_SIZE_2=173
327
PRIMER_PAIR_PENALTY_3=0.9913
328
PRIMER_LEFT_3_PENALTY=0.702456
329
PRIMER_RIGHT_3_PENALTY=0.288804
330
PRIMER_LEFT_3_SEQUENCE=CATGTTCGTGCAAACACTCA
331
PRIMER_RIGHT_3_SEQUENCE=ACCCTGTCTCCAAAAGCAAA
332
PRIMER_LEFT_3=129,20
333
PRIMER_RIGHT_3=300,20
334
PRIMER_LEFT_3_TM=59.298
335
PRIMER_RIGHT_3_TM=59.711
336
PRIMER_LEFT_3_GC_PERCENT=45.000
337
PRIMER_RIGHT_3_GC_PERCENT=45.000
338
PRIMER_LEFT_3_SELF_ANY=6.00
339
PRIMER_RIGHT_3_SELF_ANY=2.00
340
PRIMER_LEFT_3_SELF_END=1.00
341
PRIMER_RIGHT_3_SELF_END=0.00
342
PRIMER_LEFT_3_END_STABILITY=6.4000
343
PRIMER_RIGHT_3_END_STABILITY=8.8000
344
PRIMER_PAIR_3_COMPL_ANY=4.00
345
PRIMER_PAIR_3_COMPL_END=0.00
346
PRIMER_PRODUCT_SIZE_3=172
347
PRIMER_PAIR_PENALTY_4=1.2687
348
PRIMER_LEFT_4_PENALTY=1.112672
349
PRIMER_RIGHT_4_PENALTY=0.156061
350
PRIMER_LEFT_4_SEQUENCE=TGTTCGTGCAAACACTCAAA
351
PRIMER_RIGHT_4_SEQUENCE=AGACCCTGTCTCCAAAAGCA
352
PRIMER_LEFT_4=131,20
353
PRIMER_RIGHT_4=302,20
354
PRIMER_LEFT_4_TM=58.887
355
PRIMER_RIGHT_4_TM=59.844
356
PRIMER_LEFT_4_GC_PERCENT=40.000
357
PRIMER_RIGHT_4_GC_PERCENT=50.000
358
PRIMER_LEFT_4_SELF_ANY=6.00
359
PRIMER_RIGHT_4_SELF_ANY=5.00
360
PRIMER_LEFT_4_SELF_END=0.00
361
PRIMER_RIGHT_4_SELF_END=0.00
362
PRIMER_LEFT_4_END_STABILITY=7.3000
363
PRIMER_RIGHT_4_END_STABILITY=8.5000
364
PRIMER_PAIR_4_COMPL_ANY=4.00
365
PRIMER_PAIR_4_COMPL_END=0.00
366
PRIMER_PRODUCT_SIZE_4=172
367
=
368
MARKER_NAME=05.RHAP34FA5.seq
369
SEQUENCE=NNNNNNNNNACCTAACTAAAACAGACCATAAACTGAGAACCTAGTATTCATGCAGCTGAGACTTTGAAGGCCACTCTCATTCAGCCAGGTGTGTGAGGANCGCGCGGCCGACATCTACTACTACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNACAACNNNNNNNNNNNNNNNNCACAAAGNNNNNNNNNNNNNNGTAGGACAGACAGAGATTAGACAGAGTAGTAAGGGGAGAGANNAGGTTGGTTGTGGGAAGGACCTTATTACTTCACAA
370
INCLUDED_REGION=9,297
371
TARGET=125,36,AC
372
TARGET=162,24,GA
373
TARGET=191,16,AG
374
TARGET=214,14,GA
375
PRIMER_COMMENT=CL is GCGGCCGACATCTACTACTA
376
PRIMER_COMMENT=CR is TCTCTCCCCTTACTACTCTGTCTAA
377
PRIMER_PAIR_PENALTY=9.8843
378
PRIMER_LEFT_PENALTY=1.973952
379
PRIMER_RIGHT_PENALTY=7.910390
380
PRIMER_LEFT_SEQUENCE=GCGGCCGACATCTACTACTA
381
PRIMER_RIGHT_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
382
PRIMER_LEFT=103,20
383
PRIMER_RIGHT=268,25
384
PRIMER_LEFT_TM=58.026
385
PRIMER_RIGHT_TM=57.090
386
PRIMER_LEFT_GC_PERCENT=55.000
387
PRIMER_RIGHT_GC_PERCENT=44.000
388
PRIMER_LEFT_SELF_ANY=6.00
389
PRIMER_RIGHT_SELF_ANY=3.00
390
PRIMER_LEFT_SELF_END=4.00
391
PRIMER_RIGHT_SELF_END=1.00
392
PRIMER_LEFT_END_STABILITY=4.7000
393
PRIMER_RIGHT_END_STABILITY=6.0000
394
PRIMER_PAIR_COMPL_ANY=4.00
395
PRIMER_PAIR_COMPL_END=1.00
396
PRIMER_PRODUCT_SIZE=166
397
PRIMER_PAIR_PENALTY_1=10.0123
398
PRIMER_LEFT_1_PENALTY=2.101866
399
PRIMER_RIGHT_1_PENALTY=7.910390
400
PRIMER_LEFT_1_SEQUENCE=GCGGCCGACATCTACTACTAC
401
PRIMER_RIGHT_1_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
402
PRIMER_LEFT_1=103,21
403
PRIMER_RIGHT_1=268,25
404
PRIMER_LEFT_1_TM=58.898
405
PRIMER_RIGHT_1_TM=57.090
406
PRIMER_LEFT_1_GC_PERCENT=57.143
407
PRIMER_RIGHT_1_GC_PERCENT=44.000
408
PRIMER_LEFT_1_SELF_ANY=6.00
409
PRIMER_RIGHT_1_SELF_ANY=3.00
410
PRIMER_LEFT_1_SELF_END=2.00
411
PRIMER_RIGHT_1_SELF_END=1.00
412
PRIMER_LEFT_1_END_STABILITY=5.1000
413
PRIMER_RIGHT_1_END_STABILITY=6.0000
414
PRIMER_PAIR_1_COMPL_ANY=4.00
415
PRIMER_PAIR_1_COMPL_END=0.00
416
PRIMER_PRODUCT_SIZE_1=166
417
PRIMER_PAIR_PENALTY_2=10.0792
418
PRIMER_LEFT_2_PENALTY=2.168831
419
PRIMER_RIGHT_2_PENALTY=7.910390
420
PRIMER_LEFT_2_SEQUENCE=GCGGCCGACATCTACTACTACT
421
PRIMER_RIGHT_2_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
422
PRIMER_LEFT_2=103,22
423
PRIMER_RIGHT_2=268,25
424
PRIMER_LEFT_2_TM=59.831
425
PRIMER_RIGHT_2_TM=57.090
426
PRIMER_LEFT_2_GC_PERCENT=54.545
427
PRIMER_RIGHT_2_GC_PERCENT=44.000
428
PRIMER_LEFT_2_SELF_ANY=6.00
429
PRIMER_RIGHT_2_SELF_ANY=3.00
430
PRIMER_LEFT_2_SELF_END=3.00
431
PRIMER_RIGHT_2_SELF_END=1.00
432
PRIMER_LEFT_2_END_STABILITY=5.4000
433
PRIMER_RIGHT_2_END_STABILITY=6.0000
434
PRIMER_PAIR_2_COMPL_ANY=4.00
435
PRIMER_PAIR_2_COMPL_END=1.00
436
PRIMER_PRODUCT_SIZE_2=166
437
PRIMER_PAIR_PENALTY_3=10.5142
438
PRIMER_LEFT_3_PENALTY=2.603822
439
PRIMER_RIGHT_3_PENALTY=7.910390
440
PRIMER_LEFT_3_SEQUENCE=CGCGGCCGACATCTACTACT
441
PRIMER_RIGHT_3_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
442
PRIMER_LEFT_3=102,20
443
PRIMER_RIGHT_3=268,25
444
PRIMER_LEFT_3_TM=62.604
445
PRIMER_RIGHT_3_TM=57.090
446
PRIMER_LEFT_3_GC_PERCENT=60.000
447
PRIMER_RIGHT_3_GC_PERCENT=44.000
448
PRIMER_LEFT_3_SELF_ANY=6.00
449
PRIMER_RIGHT_3_SELF_ANY=3.00
450
PRIMER_LEFT_3_SELF_END=2.00
451
PRIMER_RIGHT_3_SELF_END=1.00
452
PRIMER_LEFT_3_END_STABILITY=5.4000
453
PRIMER_RIGHT_3_END_STABILITY=6.0000
454
PRIMER_PAIR_3_COMPL_ANY=4.00
455
PRIMER_PAIR_3_COMPL_END=1.00
456
PRIMER_PRODUCT_SIZE_3=167
457
PRIMER_PAIR_PENALTY_4=10.6566
458
PRIMER_LEFT_4_PENALTY=2.746227
459
PRIMER_RIGHT_4_PENALTY=7.910390
460
PRIMER_LEFT_4_SEQUENCE=CGCGGCCGACATCTACTAC
461
PRIMER_RIGHT_4_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
462
PRIMER_LEFT_4=102,19
463
PRIMER_RIGHT_4=268,25
464
PRIMER_LEFT_4_TM=61.746
465
PRIMER_RIGHT_4_TM=57.090
466
PRIMER_LEFT_4_GC_PERCENT=63.158
467
PRIMER_RIGHT_4_GC_PERCENT=44.000
468
PRIMER_LEFT_4_SELF_ANY=6.00
469
PRIMER_RIGHT_4_SELF_ANY=3.00
470
PRIMER_LEFT_4_SELF_END=1.00
471
PRIMER_RIGHT_4_SELF_END=1.00
472
PRIMER_LEFT_4_END_STABILITY=5.1000
473
PRIMER_RIGHT_4_END_STABILITY=6.0000
474
PRIMER_PAIR_4_COMPL_ANY=4.00
475
PRIMER_PAIR_4_COMPL_END=0.00
476
PRIMER_PRODUCT_SIZE_4=167
477
=
478
MARKER_NAME=06.RHAP34FE6.seq
479
SEQUENCE=NNNNNNNNCCAACCCAAAAATACAGACGGANACACACACAAACAGAGACACAGACACAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGAGACGGGTAACACAGGGNATCTTGACTGATATAGAGGTACATGTCCAAACAAGAATAGGGTAGCGGGAAAGCACAGAAGAGAAGAAAAGACAGAGTTTTGCAGACCTTGGATTTTACCCTTATGCCACCTCACCAAAGATGTGATTCCCTATATGGTCAAAGA
480
INCLUDED_REGION=8,287
481
TARGET=61,68,CA
482
PRIMER_COMMENT=CL is CAACCCAAAAATACAGACGGA
483
PRIMER_COMMENT=CR is CCCTGTGTTACCCGTCTCAA
484
PRIMER_PAIR_PENALTY=2.0965
485
PRIMER_LEFT_PENALTY=1.153194
486
PRIMER_RIGHT_PENALTY=0.943292
487
PRIMER_LEFT_SEQUENCE=CAACCCAAAAATACAGACGGA
488
PRIMER_RIGHT_SEQUENCE=CCCTGTGTTACCCGTCTCAA
489
PRIMER_LEFT=9,21
490
PRIMER_RIGHT=148,20
491
PRIMER_LEFT_TM=59.847
492
PRIMER_RIGHT_TM=60.943
493
PRIMER_LEFT_GC_PERCENT=42.857
494
PRIMER_RIGHT_GC_PERCENT=55.000
495
PRIMER_LEFT_SELF_ANY=2.00
496
PRIMER_RIGHT_SELF_ANY=3.00
497
PRIMER_LEFT_SELF_END=0.00
498
PRIMER_RIGHT_SELF_END=0.00
499
PRIMER_LEFT_END_STABILITY=9.6000
500
PRIMER_RIGHT_END_STABILITY=7.0000
501
PRIMER_PAIR_COMPL_ANY=6.00
502
PRIMER_PAIR_COMPL_END=4.00
503
PRIMER_PRODUCT_SIZE=140
504
PRIMER_PAIR_PENALTY_1=2.6236
505
PRIMER_LEFT_1_PENALTY=1.153194
506
PRIMER_RIGHT_1_PENALTY=1.470379
507
PRIMER_LEFT_1_SEQUENCE=CAACCCAAAAATACAGACGGA
508
PRIMER_RIGHT_1_SEQUENCE=CCCTGTGTTACCCGTCTCA
509
PRIMER_LEFT_1=9,21
510
PRIMER_RIGHT_1=148,19
511
PRIMER_LEFT_1_TM=59.847
512
PRIMER_RIGHT_1_TM=59.530
513
PRIMER_LEFT_1_GC_PERCENT=42.857
514
PRIMER_RIGHT_1_GC_PERCENT=57.895
515
PRIMER_LEFT_1_SELF_ANY=2.00
516
PRIMER_RIGHT_1_SELF_ANY=3.00
517
PRIMER_LEFT_1_SELF_END=0.00
518
PRIMER_RIGHT_1_SELF_END=2.00
519
PRIMER_LEFT_1_END_STABILITY=9.6000
520
PRIMER_RIGHT_1_END_STABILITY=6.7000
521
PRIMER_PAIR_1_COMPL_ANY=6.00
522
PRIMER_PAIR_1_COMPL_END=3.00
523
PRIMER_PRODUCT_SIZE_1=140
524
PRIMER_PAIR_PENALTY_2=2.9431
525
PRIMER_LEFT_2_PENALTY=1.999797
526
PRIMER_RIGHT_2_PENALTY=0.943292
527
PRIMER_LEFT_2_SEQUENCE=CCAACCCAAAAATACAGACG
528
PRIMER_RIGHT_2_SEQUENCE=CCCTGTGTTACCCGTCTCAA
529
PRIMER_LEFT_2=8,20
530
PRIMER_RIGHT_2=148,20
531
PRIMER_LEFT_2_TM=58.000
532
PRIMER_RIGHT_2_TM=60.943
533
PRIMER_LEFT_2_GC_PERCENT=45.000
534
PRIMER_RIGHT_2_GC_PERCENT=55.000
535
PRIMER_LEFT_2_SELF_ANY=2.00
536
PRIMER_RIGHT_2_SELF_ANY=3.00
537
PRIMER_LEFT_2_SELF_END=2.00
538
PRIMER_RIGHT_2_SELF_END=0.00
539
PRIMER_LEFT_2_END_STABILITY=8.1000
540
PRIMER_RIGHT_2_END_STABILITY=7.0000
541
PRIMER_PAIR_2_COMPL_ANY=5.00
542
PRIMER_PAIR_2_COMPL_END=4.00
543
PRIMER_PRODUCT_SIZE_2=141
544
PRIMER_PAIR_PENALTY_3=2.9431
545
PRIMER_LEFT_3_PENALTY=1.999797
546
PRIMER_RIGHT_3_PENALTY=0.943292
547
PRIMER_LEFT_3_SEQUENCE=CAACCCAAAAATACAGACGG
548
PRIMER_RIGHT_3_SEQUENCE=CCCTGTGTTACCCGTCTCAA
549
PRIMER_LEFT_3=9,20
550
PRIMER_RIGHT_3=148,20
551
PRIMER_LEFT_3_TM=58.000
552
PRIMER_RIGHT_3_TM=60.943
553
PRIMER_LEFT_3_GC_PERCENT=45.000
554
PRIMER_RIGHT_3_GC_PERCENT=55.000
555
PRIMER_LEFT_3_SELF_ANY=2.00
556
PRIMER_RIGHT_3_SELF_ANY=3.00
557
PRIMER_LEFT_3_SELF_END=1.00
558
PRIMER_RIGHT_3_SELF_END=0.00
559
PRIMER_LEFT_3_END_STABILITY=9.6000
560
PRIMER_RIGHT_3_END_STABILITY=7.0000
561
PRIMER_PAIR_3_COMPL_ANY=6.00
562
PRIMER_PAIR_3_COMPL_END=5.00
563
PRIMER_PRODUCT_SIZE_3=140
564
PRIMER_PAIR_PENALTY_4=3.4069
565
PRIMER_LEFT_4_PENALTY=2.463612
566
PRIMER_RIGHT_4_PENALTY=0.943292
567
PRIMER_LEFT_4_SEQUENCE=CCAACCCAAAAATACAGACGG
568
PRIMER_RIGHT_4_SEQUENCE=CCCTGTGTTACCCGTCTCAA
569
PRIMER_LEFT_4=8,21
570
PRIMER_RIGHT_4=148,20
571
PRIMER_LEFT_4_TM=61.464
572
PRIMER_RIGHT_4_TM=60.943
573
PRIMER_LEFT_4_GC_PERCENT=47.619
574
PRIMER_RIGHT_4_GC_PERCENT=55.000
575
PRIMER_LEFT_4_SELF_ANY=2.00
576
PRIMER_RIGHT_4_SELF_ANY=3.00
577
PRIMER_LEFT_4_SELF_END=1.00
578
PRIMER_RIGHT_4_SELF_END=0.00
579
PRIMER_LEFT_4_END_STABILITY=9.6000
580
PRIMER_RIGHT_4_END_STABILITY=7.0000
581
PRIMER_PAIR_4_COMPL_ANY=6.00
582
PRIMER_PAIR_4_COMPL_END=5.00
583
PRIMER_PRODUCT_SIZE_4=141
584
=
585
MARKER_NAME=07.RHAP34FE7.seq
586
SEQUENCE=NNNNNNNNNNNNNNNNNNCCAGGACTGGAGTTCTCAACTGGAAGCAGGGCATAATAAATATACACAGACTACTCTTTGGGTACAAACTGACAGGCAATTTCAAGGCTTAAAGTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCTCATGCACACCCCCCCTCTCCACCTGAACCTCTACATGCGTGCACATGTTCACACATGTTCTCTCATTTGCTCTCACACTTGCTCTCATTACTCGCTCTCTAGACCTT
587
INCLUDED_REGION=18,283
588
TARGET=115,29,CT
589
TARGET=145,20,NG
590
TARGET=166,24,CA
591
PRIMER_COMMENT=CL is TGGGTACAAACTGACAGGCA
592
PRIMER_COMMENT=CR is TGTGCACGCATGTAGAGGTT
593
PRIMER_PAIR_PENALTY=0.4846
594
PRIMER_LEFT_PENALTY=0.151542
595
PRIMER_RIGHT_PENALTY=0.333053
596
PRIMER_LEFT_SEQUENCE=TGGGTACAAACTGACAGGCA
597
PRIMER_RIGHT_SEQUENCE=TGTGCACGCATGTAGAGGTT
598
PRIMER_LEFT=76,20
599
PRIMER_RIGHT=238,20
600
PRIMER_LEFT_TM=60.152
601
PRIMER_RIGHT_TM=60.333
602
PRIMER_LEFT_GC_PERCENT=50.000
603
PRIMER_RIGHT_GC_PERCENT=50.000
604
PRIMER_LEFT_SELF_ANY=5.00
605
PRIMER_RIGHT_SELF_ANY=6.00
606
PRIMER_LEFT_SELF_END=3.00
607
PRIMER_RIGHT_SELF_END=0.00
608
PRIMER_LEFT_END_STABILITY=9.7000
609
PRIMER_RIGHT_END_STABILITY=7.9000
610
PRIMER_PAIR_COMPL_ANY=4.00
611
PRIMER_PAIR_COMPL_END=3.00
612
PRIMER_PRODUCT_SIZE=163
613
PRIMER_PAIR_PENALTY_1=0.6812
614
PRIMER_LEFT_1_PENALTY=0.151542
615
PRIMER_RIGHT_1_PENALTY=0.529649
616
PRIMER_LEFT_1_SEQUENCE=TGGGTACAAACTGACAGGCA
617
PRIMER_RIGHT_1_SEQUENCE=GCACGCATGTAGAGGTTCAG
618
PRIMER_LEFT_1=76,20
619
PRIMER_RIGHT_1=235,20
620
PRIMER_LEFT_1_TM=60.152
621
PRIMER_RIGHT_1_TM=59.470
622
PRIMER_LEFT_1_GC_PERCENT=50.000
623
PRIMER_RIGHT_1_GC_PERCENT=55.000
624
PRIMER_LEFT_1_SELF_ANY=5.00
625
PRIMER_RIGHT_1_SELF_ANY=4.00
626
PRIMER_LEFT_1_SELF_END=3.00
627
PRIMER_RIGHT_1_SELF_END=1.00
628
PRIMER_LEFT_1_END_STABILITY=9.7000
629
PRIMER_RIGHT_1_END_STABILITY=7.0000
630
PRIMER_PAIR_1_COMPL_ANY=4.00
631
PRIMER_PAIR_1_COMPL_END=0.00
632
PRIMER_PRODUCT_SIZE_1=160
633
PRIMER_PAIR_PENALTY_2=0.7897
634
PRIMER_LEFT_2_PENALTY=0.151542
635
PRIMER_RIGHT_2_PENALTY=0.638191
636
PRIMER_LEFT_2_SEQUENCE=TGGGTACAAACTGACAGGCA
637
PRIMER_RIGHT_2_SEQUENCE=AGAGGTTCAGGTGGAGAGGG
638
PRIMER_LEFT_2=76,20
639
PRIMER_RIGHT_2=225,20
640
PRIMER_LEFT_2_TM=60.152
641
PRIMER_RIGHT_2_TM=60.638
642
PRIMER_LEFT_2_GC_PERCENT=50.000
643
PRIMER_RIGHT_2_GC_PERCENT=60.000
644
PRIMER_LEFT_2_SELF_ANY=5.00
645
PRIMER_RIGHT_2_SELF_ANY=2.00
646
PRIMER_LEFT_2_SELF_END=3.00
647
PRIMER_RIGHT_2_SELF_END=0.00
648
PRIMER_LEFT_2_END_STABILITY=9.7000
649
PRIMER_RIGHT_2_END_STABILITY=9.4000
650
PRIMER_PAIR_2_COMPL_ANY=4.00
651
PRIMER_PAIR_2_COMPL_END=0.00
652
PRIMER_PRODUCT_SIZE_2=150
653
PRIMER_PAIR_PENALTY_3=0.8285
654
PRIMER_LEFT_3_PENALTY=0.151542
655
PRIMER_RIGHT_3_PENALTY=0.676947
656
PRIMER_LEFT_3_SEQUENCE=TGGGTACAAACTGACAGGCA
657
PRIMER_RIGHT_3_SEQUENCE=GTGCACGCATGTAGAGGTTC
658
PRIMER_LEFT_3=76,20
659
PRIMER_RIGHT_3=237,20
660
PRIMER_LEFT_3_TM=60.152
661
PRIMER_RIGHT_3_TM=59.323
662
PRIMER_LEFT_3_GC_PERCENT=50.000
663
PRIMER_RIGHT_3_GC_PERCENT=55.000
664
PRIMER_LEFT_3_SELF_ANY=5.00
665
PRIMER_RIGHT_3_SELF_ANY=6.00
666
PRIMER_LEFT_3_SELF_END=3.00
667
PRIMER_RIGHT_3_SELF_END=1.00
668
PRIMER_LEFT_3_END_STABILITY=9.7000
669
PRIMER_RIGHT_3_END_STABILITY=7.9000
670
PRIMER_PAIR_3_COMPL_ANY=4.00
671
PRIMER_PAIR_3_COMPL_END=2.00
672
PRIMER_PRODUCT_SIZE_3=162
673
PRIMER_PAIR_PENALTY_4=0.8378
674
PRIMER_LEFT_4_PENALTY=0.151542
675
PRIMER_RIGHT_4_PENALTY=0.686256
676
PRIMER_LEFT_4_SEQUENCE=TGGGTACAAACTGACAGGCA
677
PRIMER_RIGHT_4_SEQUENCE=CACGCATGTAGAGGTTCAGG
678
PRIMER_LEFT_4=76,20
679
PRIMER_RIGHT_4=234,20
680
PRIMER_LEFT_4_TM=60.152
681
PRIMER_RIGHT_4_TM=59.314
682
PRIMER_LEFT_4_GC_PERCENT=50.000
683
PRIMER_RIGHT_4_GC_PERCENT=55.000
684
PRIMER_LEFT_4_SELF_ANY=5.00
685
PRIMER_RIGHT_4_SELF_ANY=4.00
686
PRIMER_LEFT_4_SELF_END=3.00
687
PRIMER_RIGHT_4_SELF_END=0.00
688
PRIMER_LEFT_4_END_STABILITY=9.7000
689
PRIMER_RIGHT_4_END_STABILITY=8.2000
690
PRIMER_PAIR_4_COMPL_ANY=4.00
691
PRIMER_PAIR_4_COMPL_END=0.00
692
PRIMER_PRODUCT_SIZE_4=159
693
=
694
MARKER_NAME=08.RHAP34FE8.seq
695
SEQUENCE=NNNNNNNNNNNNNNNNNNNNCATGCAGTGTGAATATCTCATGTTCTTCAGCTTTTTCCCCTTAGGACCAAGGTATAGCCTTAGGACCACGGATTATCCTTCCATTTTCTTAGAACTCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTGAGTGGCATCTGTCCCACCAGGTCCTTTGAAGAGCTGAGTGAAATGATGTATAAAGTACTTTGCATTGGCAAGGGGGAAGACTCCAGCATGGATCTAGTAGAAGAAATATGAAGGAG
696
INCLUDED_REGION=20,281
697
TARGET=119,62,AC
698
PRIMER_COMMENT=CL is TTTCCCCTTAGGACCAAGGT
699
PRIMER_COMMENT=CR is CAGCTCTTCAAAGGACCTGG
700
PRIMER_PAIR_PENALTY=0.2190
701
PRIMER_LEFT_PENALTY=0.201280
702
PRIMER_RIGHT_PENALTY=0.017711
703
PRIMER_LEFT_SEQUENCE=TTTCCCCTTAGGACCAAGGT
704
PRIMER_RIGHT_SEQUENCE=CAGCTCTTCAAAGGACCTGG
705
PRIMER_LEFT=53,20
706
PRIMER_RIGHT=220,20
707
PRIMER_LEFT_TM=59.799
708
PRIMER_RIGHT_TM=59.982
709
PRIMER_LEFT_GC_PERCENT=50.000
710
PRIMER_RIGHT_GC_PERCENT=55.000
711
PRIMER_LEFT_SELF_ANY=7.00
712
PRIMER_RIGHT_SELF_ANY=6.00
713
PRIMER_LEFT_SELF_END=5.00
714
PRIMER_RIGHT_SELF_END=4.00
715
PRIMER_LEFT_END_STABILITY=7.9000
716
PRIMER_RIGHT_END_STABILITY=9.7000
717
PRIMER_PAIR_COMPL_ANY=4.00
718
PRIMER_PAIR_COMPL_END=4.00
719
PRIMER_PRODUCT_SIZE=168
720
PRIMER_PAIR_PENALTY_1=0.2677
721
PRIMER_LEFT_1_PENALTY=0.249989
722
PRIMER_RIGHT_1_PENALTY=0.017711
723
PRIMER_LEFT_1_SEQUENCE=GACCACGGATTATCCTTCCA
724
PRIMER_RIGHT_1_SEQUENCE=CAGCTCTTCAAAGGACCTGG
725
PRIMER_LEFT_1=83,20
726
PRIMER_RIGHT_1=220,20
727
PRIMER_LEFT_1_TM=59.750
728
PRIMER_RIGHT_1_TM=59.982
729
PRIMER_LEFT_1_GC_PERCENT=50.000
730
PRIMER_RIGHT_1_GC_PERCENT=55.000
731
PRIMER_LEFT_1_SELF_ANY=7.00
732
PRIMER_RIGHT_1_SELF_ANY=6.00
733
PRIMER_LEFT_1_SELF_END=1.00
734
PRIMER_RIGHT_1_SELF_END=4.00
735
PRIMER_LEFT_1_END_STABILITY=8.5000
736
PRIMER_RIGHT_1_END_STABILITY=9.7000
737
PRIMER_PAIR_1_COMPL_ANY=5.00
738
PRIMER_PAIR_1_COMPL_END=3.00
739
PRIMER_PRODUCT_SIZE_1=138
740
PRIMER_PAIR_PENALTY_2=0.3573
741
PRIMER_LEFT_2_PENALTY=0.201280
742
PRIMER_RIGHT_2_PENALTY=0.156061
743
PRIMER_LEFT_2_SEQUENCE=TTTCCCCTTAGGACCAAGGT
744
PRIMER_RIGHT_2_SEQUENCE=GCTCTTCAAAGGACCTGGTG
745
PRIMER_LEFT_2=53,20
746
PRIMER_RIGHT_2=218,20
747
PRIMER_LEFT_2_TM=59.799
748
PRIMER_RIGHT_2_TM=59.844
749
PRIMER_LEFT_2_GC_PERCENT=50.000
750
PRIMER_RIGHT_2_GC_PERCENT=55.000
751
PRIMER_LEFT_2_SELF_ANY=7.00
752
PRIMER_RIGHT_2_SELF_ANY=5.00
753
PRIMER_LEFT_2_SELF_END=5.00
754
PRIMER_RIGHT_2_SELF_END=3.00
755
PRIMER_LEFT_2_END_STABILITY=7.9000
756
PRIMER_RIGHT_2_END_STABILITY=8.2000
757
PRIMER_PAIR_2_COMPL_ANY=5.00
758
PRIMER_PAIR_2_COMPL_END=4.00
759
PRIMER_PRODUCT_SIZE_2=166
760
PRIMER_PAIR_PENALTY_3=0.4060
761
PRIMER_LEFT_3_PENALTY=0.249989
762
PRIMER_RIGHT_3_PENALTY=0.156061
763
PRIMER_LEFT_3_SEQUENCE=GACCACGGATTATCCTTCCA
764
PRIMER_RIGHT_3_SEQUENCE=GCTCTTCAAAGGACCTGGTG
765
PRIMER_LEFT_3=83,20
766
PRIMER_RIGHT_3=218,20
767
PRIMER_LEFT_3_TM=59.750
768
PRIMER_RIGHT_3_TM=59.844
769
PRIMER_LEFT_3_GC_PERCENT=50.000
770
PRIMER_RIGHT_3_GC_PERCENT=55.000
771
PRIMER_LEFT_3_SELF_ANY=7.00
772
PRIMER_RIGHT_3_SELF_ANY=5.00
773
PRIMER_LEFT_3_SELF_END=1.00
774
PRIMER_RIGHT_3_SELF_END=3.00
775
PRIMER_LEFT_3_END_STABILITY=8.5000
776
PRIMER_RIGHT_3_END_STABILITY=8.2000
777
PRIMER_PAIR_3_COMPL_ANY=5.00
778
PRIMER_PAIR_3_COMPL_END=2.00
779
PRIMER_PRODUCT_SIZE_3=136
780
PRIMER_PAIR_PENALTY_4=0.4406
781
PRIMER_LEFT_4_PENALTY=0.422888
782
PRIMER_RIGHT_4_PENALTY=0.017711
783
PRIMER_LEFT_4_SEQUENCE=GCTTTTTCCCCTTAGGACCA
784
PRIMER_RIGHT_4_SEQUENCE=CAGCTCTTCAAAGGACCTGG
785
PRIMER_LEFT_4=49,20
786
PRIMER_RIGHT_4=220,20
787
PRIMER_LEFT_4_TM=60.423
788
PRIMER_RIGHT_4_TM=59.982
789
PRIMER_LEFT_4_GC_PERCENT=50.000
790
PRIMER_RIGHT_4_GC_PERCENT=55.000
791
PRIMER_LEFT_4_SELF_ANY=5.00
792
PRIMER_RIGHT_4_SELF_ANY=6.00
793
PRIMER_LEFT_4_SELF_END=1.00
794
PRIMER_RIGHT_4_SELF_END=4.00
795
PRIMER_LEFT_4_END_STABILITY=7.9000
796
PRIMER_RIGHT_4_END_STABILITY=9.7000
797
PRIMER_PAIR_4_COMPL_ANY=4.00
798
PRIMER_PAIR_4_COMPL_END=3.00
799
PRIMER_PRODUCT_SIZE_4=172
800
=
801
MARKER_NAME=09.RHAP33FA9.seq
802
SEQUENCE=GCCTTGGGATTTTACTTATTGAGACATCCTGTTACCATGTAGGAGTTTAGAGAGGTTTCATTTTCTCTGCTAAGTAATGAGTTCAAAGCCAGGAAATCATCTCAGAGACAGCAGGCAGCAATAAGAAACACATTTTATTTCAGTTTATGAANNNNNNNNNNNNNNNNNNAGGGGGGAGGGAGGGAGGGACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCNNNNNNNNNNNNNNNNNCANNNCNCNCTGNAGCANAANCNCCCTGCAAGNGCCGNCGNNGTNNCNGCNNTNNNNTCCCCCNGCNGGNGNNCCGNGCNCCCNNCCGGCGNNNCNNCNTGCNGNNG
803
INCLUDED_REGION=0,371
804
TARGET=151,18,AC
805
TARGET=192,53,GA
806
TARGET=248,16,AG
807
PRIMER_COMMENT=CL is TGAGACATCCTGTTACCATGTAGG
808
PRIMER_COMMENT=CR is CTGTCCCTCCCTCCCTCC
809
PRIMER_PAIR_PENALTY=8.2998
810
PRIMER_LEFT_PENALTY=4.294975
811
PRIMER_RIGHT_PENALTY=4.004782
812
PRIMER_LEFT_SEQUENCE=TGAGACATCCTGTTACCATGTAGG
813
PRIMER_RIGHT_SEQUENCE=CTGTCCCTCCCTCCCTCC
814
PRIMER_LEFT=19,24
815
PRIMER_RIGHT=191,18
816
PRIMER_LEFT_TM=60.295
817
PRIMER_RIGHT_TM=62.005
818
PRIMER_LEFT_GC_PERCENT=45.833
819
PRIMER_RIGHT_GC_PERCENT=72.222
820
PRIMER_LEFT_SELF_ANY=4.00
821
PRIMER_RIGHT_SELF_ANY=1.00
822
PRIMER_LEFT_SELF_END=2.00
823
PRIMER_RIGHT_SELF_END=0.00
824
PRIMER_LEFT_END_STABILITY=6.9000
825
PRIMER_RIGHT_END_STABILITY=9.4000
826
PRIMER_PAIR_COMPL_ANY=4.00
827
PRIMER_PAIR_COMPL_END=3.00
828
PRIMER_PRODUCT_SIZE=173
829
PRIMER_PAIR_PENALTY_1=8.3060
830
PRIMER_LEFT_1_PENALTY=4.301260
831
PRIMER_RIGHT_1_PENALTY=4.004782
832
PRIMER_LEFT_1_SEQUENCE=TGCTAAGTAATGAGTTCAAAGCCA
833
PRIMER_RIGHT_1_SEQUENCE=CTGTCCCTCCCTCCCTCC
834
PRIMER_LEFT_1=67,24
835
PRIMER_RIGHT_1=191,18
836
PRIMER_LEFT_1_TM=60.301
837
PRIMER_RIGHT_1_TM=62.005
838
PRIMER_LEFT_1_GC_PERCENT=37.500
839
PRIMER_RIGHT_1_GC_PERCENT=72.222
840
PRIMER_LEFT_1_SELF_ANY=4.00
841
PRIMER_RIGHT_1_SELF_ANY=1.00
842
PRIMER_LEFT_1_SELF_END=2.00
843
PRIMER_RIGHT_1_SELF_END=0.00
844
PRIMER_LEFT_1_END_STABILITY=9.7000
845
PRIMER_RIGHT_1_END_STABILITY=9.4000
846
PRIMER_PAIR_1_COMPL_ANY=3.00
847
PRIMER_PAIR_1_COMPL_END=0.00
848
PRIMER_PRODUCT_SIZE_1=125
849
PRIMER_PAIR_PENALTY_2=8.5894
850
PRIMER_LEFT_2_PENALTY=4.584629
851
PRIMER_RIGHT_2_PENALTY=4.004782
852
PRIMER_LEFT_2_SEQUENCE=GAGACATCCTGTTACCATGTAGGA
853
PRIMER_RIGHT_2_SEQUENCE=CTGTCCCTCCCTCCCTCC
854
PRIMER_LEFT_2=20,24
855
PRIMER_RIGHT_2=191,18
856
PRIMER_LEFT_2_TM=59.415
857
PRIMER_RIGHT_2_TM=62.005
858
PRIMER_LEFT_2_GC_PERCENT=45.833
859
PRIMER_RIGHT_2_GC_PERCENT=72.222
860
PRIMER_LEFT_2_SELF_ANY=4.00
861
PRIMER_RIGHT_2_SELF_ANY=1.00
862
PRIMER_LEFT_2_SELF_END=2.00
863
PRIMER_RIGHT_2_SELF_END=0.00
864
PRIMER_LEFT_2_END_STABILITY=7.2000
865
PRIMER_RIGHT_2_END_STABILITY=9.4000
866
PRIMER_PAIR_2_COMPL_ANY=4.00
867
PRIMER_PAIR_2_COMPL_END=3.00
868
PRIMER_PRODUCT_SIZE_2=172
869
PRIMER_PAIR_PENALTY_3=8.9567
870
PRIMER_LEFT_3_PENALTY=4.951910
871
PRIMER_RIGHT_3_PENALTY=4.004782
872
PRIMER_LEFT_3_SEQUENCE=TTGAGACATCCTGTTACCATGT
873
PRIMER_RIGHT_3_SEQUENCE=CTGTCCCTCCCTCCCTCC
874
PRIMER_LEFT_3=18,22
875
PRIMER_RIGHT_3=191,18
876
PRIMER_LEFT_3_TM=57.048
877
PRIMER_RIGHT_3_TM=62.005
878
PRIMER_LEFT_3_GC_PERCENT=40.909
879
PRIMER_RIGHT_3_GC_PERCENT=72.222
880
PRIMER_LEFT_3_SELF_ANY=4.00
881
PRIMER_RIGHT_3_SELF_ANY=1.00
882
PRIMER_LEFT_3_SELF_END=3.00
883
PRIMER_RIGHT_3_SELF_END=0.00
884
PRIMER_LEFT_3_END_STABILITY=6.6000
885
PRIMER_RIGHT_3_END_STABILITY=9.4000
886
PRIMER_PAIR_3_COMPL_ANY=4.00
887
PRIMER_PAIR_3_COMPL_END=0.00
888
PRIMER_PRODUCT_SIZE_3=174
889
PRIMER_PAIR_PENALTY_4=9.1359
890
PRIMER_LEFT_4_PENALTY=5.131103
891
PRIMER_RIGHT_4_PENALTY=4.004782
892
PRIMER_LEFT_4_SEQUENCE=AGAGAGGTTTCATTTTCTCTGCT
893
PRIMER_RIGHT_4_SEQUENCE=CTGTCCCTCCCTCCCTCC
894
PRIMER_LEFT_4=48,23
895
PRIMER_RIGHT_4=191,18
896
PRIMER_LEFT_4_TM=57.869
897
PRIMER_RIGHT_4_TM=62.005
898
PRIMER_LEFT_4_GC_PERCENT=39.130
899
PRIMER_RIGHT_4_GC_PERCENT=72.222
900
PRIMER_LEFT_4_SELF_ANY=5.00
901
PRIMER_RIGHT_4_SELF_ANY=1.00
902
PRIMER_LEFT_4_SELF_END=0.00
903
PRIMER_RIGHT_4_SELF_END=0.00
904
PRIMER_LEFT_4_END_STABILITY=8.2000
905
PRIMER_RIGHT_4_END_STABILITY=9.4000
906
PRIMER_PAIR_4_COMPL_ANY=5.00
907
PRIMER_PAIR_4_COMPL_END=0.00
908
PRIMER_PRODUCT_SIZE_4=144
909
=
910
MARKER_NAME=09.RHAP34FA9.seq
911
SEQUENCE=NNNNNNNNNNNNNNNNNNNNTGATCAGGATTTCAGTGCTAATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCCTTTGGTAACTCCTGTTGTACAAGGGCTCCAAAAAGCAGGAAAGAATCAGAAACAGCCCCTGTTCCCTCTGTGAGGAAACCTACAAAAATGCAAGCAACACAAATATAATGTGTATACAGATAGCTTAGGTTAGATTCAAGCAGGCTCTTGTATTTTCAGCTCAGTCTCAGTGTAGCACCTGTAAATGCAGGTTGATTGAGGTTTATTTAT
912
INCLUDED_REGION=20,280
913
TARGET=44,42,CA
914
PRIMER_COMMENT=CL is TGATCAGGATTTCAGTGCTAATAT
915
PRIMER_COMMENT=CR is TTCCTCACAGAGGGAACAGG
916
PRIMER_PAIR_PENALTY=7.2009
917
PRIMER_LEFT_PENALTY=6.971746
918
PRIMER_RIGHT_PENALTY=0.229181
919
PRIMER_LEFT_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
920
PRIMER_RIGHT_SEQUENCE=TTCCTCACAGAGGGAACAGG
921
PRIMER_LEFT=20,24
922
PRIMER_RIGHT=166,20
923
PRIMER_LEFT_TM=57.028
924
PRIMER_RIGHT_TM=60.229
925
PRIMER_LEFT_GC_PERCENT=33.333
926
PRIMER_RIGHT_GC_PERCENT=55.000
927
PRIMER_LEFT_SELF_ANY=6.00
928
PRIMER_RIGHT_SELF_ANY=5.00
929
PRIMER_LEFT_SELF_END=4.00
930
PRIMER_RIGHT_SELF_END=0.00
931
PRIMER_LEFT_END_STABILITY=5.8000
932
PRIMER_RIGHT_END_STABILITY=7.9000
933
PRIMER_PAIR_COMPL_ANY=4.00
934
PRIMER_PAIR_COMPL_END=0.00
935
PRIMER_PRODUCT_SIZE=147
936
PRIMER_PAIR_PENALTY_1=7.6879
937
PRIMER_LEFT_1_PENALTY=6.971746
938
PRIMER_RIGHT_1_PENALTY=0.716126
939
PRIMER_LEFT_1_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
940
PRIMER_RIGHT_1_SEQUENCE=CAGGGGCTGTTTCTGATTCT
941
PRIMER_LEFT_1=20,24
942
PRIMER_RIGHT_1=150,20
943
PRIMER_LEFT_1_TM=57.028
944
PRIMER_RIGHT_1_TM=59.284
945
PRIMER_LEFT_1_GC_PERCENT=33.333
946
PRIMER_RIGHT_1_GC_PERCENT=50.000
947
PRIMER_LEFT_1_SELF_ANY=6.00
948
PRIMER_RIGHT_1_SELF_ANY=3.00
949
PRIMER_LEFT_1_SELF_END=4.00
950
PRIMER_RIGHT_1_SELF_END=1.00
951
PRIMER_LEFT_1_END_STABILITY=5.8000
952
PRIMER_RIGHT_1_END_STABILITY=6.6000
953
PRIMER_PAIR_1_COMPL_ANY=5.00
954
PRIMER_PAIR_1_COMPL_END=1.00
955
PRIMER_PRODUCT_SIZE_1=131
956
PRIMER_PAIR_PENALTY_2=7.8310
957
PRIMER_LEFT_2_PENALTY=6.971746
958
PRIMER_RIGHT_2_PENALTY=0.859212
959
PRIMER_LEFT_2_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
960
PRIMER_RIGHT_2_SEQUENCE=ACAGGGGCTGTTTCTGATTC
961
PRIMER_LEFT_2=20,24
962
PRIMER_RIGHT_2=151,20
963
PRIMER_LEFT_2_TM=57.028
964
PRIMER_RIGHT_2_TM=59.141
965
PRIMER_LEFT_2_GC_PERCENT=33.333
966
PRIMER_RIGHT_2_GC_PERCENT=50.000
967
PRIMER_LEFT_2_SELF_ANY=6.00
968
PRIMER_RIGHT_2_SELF_ANY=5.00
969
PRIMER_LEFT_2_SELF_END=4.00
970
PRIMER_RIGHT_2_SELF_END=3.00
971
PRIMER_LEFT_2_END_STABILITY=5.8000
972
PRIMER_RIGHT_2_END_STABILITY=6.6000
973
PRIMER_PAIR_2_COMPL_ANY=5.00
974
PRIMER_PAIR_2_COMPL_END=0.00
975
PRIMER_PRODUCT_SIZE_2=132
976
PRIMER_PAIR_PENALTY_3=7.8310
977
PRIMER_LEFT_3_PENALTY=6.971746
978
PRIMER_RIGHT_3_PENALTY=0.859212
979
PRIMER_LEFT_3_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
980
PRIMER_RIGHT_3_SEQUENCE=GAACAGGGGCTGTTTCTGAT
981
PRIMER_LEFT_3=20,24
982
PRIMER_RIGHT_3=153,20
983
PRIMER_LEFT_3_TM=57.028
984
PRIMER_RIGHT_3_TM=59.141
985
PRIMER_LEFT_3_GC_PERCENT=33.333
986
PRIMER_RIGHT_3_GC_PERCENT=50.000
987
PRIMER_LEFT_3_SELF_ANY=6.00
988
PRIMER_RIGHT_3_SELF_ANY=7.00
989
PRIMER_LEFT_3_SELF_END=4.00
990
PRIMER_RIGHT_3_SELF_END=2.00
991
PRIMER_LEFT_3_END_STABILITY=5.8000
992
PRIMER_RIGHT_3_END_STABILITY=6.6000
993
PRIMER_PAIR_3_COMPL_ANY=5.00
994
PRIMER_PAIR_3_COMPL_END=2.00
995
PRIMER_PRODUCT_SIZE_3=134
996
PRIMER_PAIR_PENALTY_4=8.0391
997
PRIMER_LEFT_4_PENALTY=6.971746
998
PRIMER_RIGHT_4_PENALTY=1.067353
999
PRIMER_LEFT_4_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
1000
PRIMER_RIGHT_4_SEQUENCE=GGGCTGTTTCTGATTCTTTCC
1001
PRIMER_LEFT_4=20,24
1002
PRIMER_RIGHT_4=147,21
1003
PRIMER_LEFT_4_TM=57.028
1004
PRIMER_RIGHT_4_TM=60.067
1005
PRIMER_LEFT_4_GC_PERCENT=33.333
1006
PRIMER_RIGHT_4_GC_PERCENT=47.619
1007
PRIMER_LEFT_4_SELF_ANY=6.00
1008
PRIMER_RIGHT_4_SELF_ANY=3.00
1009
PRIMER_LEFT_4_SELF_END=4.00
1010
PRIMER_RIGHT_4_SELF_END=0.00
1011
PRIMER_LEFT_4_END_STABILITY=5.8000
1012
PRIMER_RIGHT_4_END_STABILITY=8.5000
1013
PRIMER_PAIR_4_COMPL_ANY=5.00
1014
PRIMER_PAIR_4_COMPL_END=0.00
1015
PRIMER_PRODUCT_SIZE_4=128
1016
=
1017
MARKER_NAME=10.RHAP34FA10.seq
1018
SEQUENCE=NNNNNNNNNNNNACACATCTGAGAGTAGGGCAACCCCTGGGGTTCAAAACAGAAAGAAAGAATTCATTATTTAGGATAGAAAGGCAAGTAAATATAGATATCCTGCAATGGGATGAATACAAAGATTTATTTATTTATATTTATATTCATTAATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATCAGAGGACAGGTTGTAGGAGCCAGTTCAGTTCTGTCCTTGCATACCTACATTCTGGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
1019
INCLUDED_REGION=12,236
1020
TARGET=155,32,TG
1021
PRIMER_COMMENT=CL is CCTGCAATGGGATGAATACA
1022
PRIMER_COMMENT=CR is CAAGGACAGAACTGAACTGGC
1023
PRIMER_PAIR_PENALTY=1.7506
1024
PRIMER_LEFT_PENALTY=0.649665
1025
PRIMER_RIGHT_PENALTY=1.100969
1026
PRIMER_LEFT_SEQUENCE=CCTGCAATGGGATGAATACA
1027
PRIMER_RIGHT_SEQUENCE=CAAGGACAGAACTGAACTGGC
1028
PRIMER_LEFT=101,20
1029
PRIMER_RIGHT=229,21
1030
PRIMER_LEFT_TM=59.350
1031
PRIMER_RIGHT_TM=59.899
1032
PRIMER_LEFT_GC_PERCENT=45.000
1033
PRIMER_RIGHT_GC_PERCENT=52.381
1034
PRIMER_LEFT_SELF_ANY=4.00
1035
PRIMER_RIGHT_SELF_ANY=4.00
1036
PRIMER_LEFT_SELF_END=4.00
1037
PRIMER_RIGHT_SELF_END=3.00
1038
PRIMER_LEFT_END_STABILITY=5.6000
1039
PRIMER_RIGHT_END_STABILITY=9.7000
1040
PRIMER_PAIR_COMPL_ANY=3.00
1041
PRIMER_PAIR_COMPL_END=0.00
1042
PRIMER_PRODUCT_SIZE=129
1043
PRIMER_PAIR_PENALTY_1=1.7506
1044
PRIMER_LEFT_1_PENALTY=0.649665
1045
PRIMER_RIGHT_1_PENALTY=1.100969
1046
PRIMER_LEFT_1_SEQUENCE=CCTGCAATGGGATGAATACA
1047
PRIMER_RIGHT_1_SEQUENCE=GCAAGGACAGAACTGAACTGG
1048
PRIMER_LEFT_1=101,20
1049
PRIMER_RIGHT_1=230,21
1050
PRIMER_LEFT_1_TM=59.350
1051
PRIMER_RIGHT_1_TM=59.899
1052
PRIMER_LEFT_1_GC_PERCENT=45.000
1053
PRIMER_RIGHT_1_GC_PERCENT=52.381
1054
PRIMER_LEFT_1_SELF_ANY=4.00
1055
PRIMER_RIGHT_1_SELF_ANY=4.00
1056
PRIMER_LEFT_1_SELF_END=4.00
1057
PRIMER_RIGHT_1_SELF_END=4.00
1058
PRIMER_LEFT_1_END_STABILITY=5.6000
1059
PRIMER_RIGHT_1_END_STABILITY=7.9000
1060
PRIMER_PAIR_1_COMPL_ANY=3.00
1061
PRIMER_PAIR_1_COMPL_END=1.00
1062
PRIMER_PRODUCT_SIZE_1=130
1063
PRIMER_PAIR_PENALTY_2=2.3642
1064
PRIMER_LEFT_2_PENALTY=1.263200
1065
PRIMER_RIGHT_2_PENALTY=1.100969
1066
PRIMER_LEFT_2_SEQUENCE=GATATCCTGCAATGGGATGAA
1067
PRIMER_RIGHT_2_SEQUENCE=CAAGGACAGAACTGAACTGGC
1068
PRIMER_LEFT_2=96,21
1069
PRIMER_RIGHT_2=229,21
1070
PRIMER_LEFT_2_TM=59.737
1071
PRIMER_RIGHT_2_TM=59.899
1072
PRIMER_LEFT_2_GC_PERCENT=42.857
1073
PRIMER_RIGHT_2_GC_PERCENT=52.381
1074
PRIMER_LEFT_2_SELF_ANY=6.00
1075
PRIMER_RIGHT_2_SELF_ANY=4.00
1076
PRIMER_LEFT_2_SELF_END=2.00
1077
PRIMER_RIGHT_2_SELF_END=3.00
1078
PRIMER_LEFT_2_END_STABILITY=6.9000
1079
PRIMER_RIGHT_2_END_STABILITY=9.7000
1080
PRIMER_PAIR_2_COMPL_ANY=4.00
1081
PRIMER_PAIR_2_COMPL_END=0.00
1082
PRIMER_PRODUCT_SIZE_2=134
1083
PRIMER_PAIR_PENALTY_3=2.3642
1084
PRIMER_LEFT_3_PENALTY=1.263200
1085
PRIMER_RIGHT_3_PENALTY=1.100969
1086
PRIMER_LEFT_3_SEQUENCE=GATATCCTGCAATGGGATGAA
1087
PRIMER_RIGHT_3_SEQUENCE=GCAAGGACAGAACTGAACTGG
1088
PRIMER_LEFT_3=96,21
1089
PRIMER_RIGHT_3=230,21
1090
PRIMER_LEFT_3_TM=59.737
1091
PRIMER_RIGHT_3_TM=59.899
1092
PRIMER_LEFT_3_GC_PERCENT=42.857
1093
PRIMER_RIGHT_3_GC_PERCENT=52.381
1094
PRIMER_LEFT_3_SELF_ANY=6.00
1095
PRIMER_RIGHT_3_SELF_ANY=4.00
1096
PRIMER_LEFT_3_SELF_END=2.00
1097
PRIMER_RIGHT_3_SELF_END=4.00
1098
PRIMER_LEFT_3_END_STABILITY=6.9000
1099
PRIMER_RIGHT_3_END_STABILITY=7.9000
1100
PRIMER_PAIR_3_COMPL_ANY=4.00
1101
PRIMER_PAIR_3_COMPL_END=0.00
1102
PRIMER_PRODUCT_SIZE_3=135
1103
PRIMER_PAIR_PENALTY_4=2.5784
1104
PRIMER_LEFT_4_PENALTY=0.649665
1105
PRIMER_RIGHT_4_PENALTY=1.928763
1106
PRIMER_LEFT_4_SEQUENCE=CCTGCAATGGGATGAATACA
1107
PRIMER_RIGHT_4_SEQUENCE=TGCAAGGACAGAACTGAACTG
1108
PRIMER_LEFT_4=101,20
1109
PRIMER_RIGHT_4=231,21
1110
PRIMER_LEFT_4_TM=59.350
1111
PRIMER_RIGHT_4_TM=59.071
1112
PRIMER_LEFT_4_GC_PERCENT=45.000
1113
PRIMER_RIGHT_4_GC_PERCENT=47.619
1114
PRIMER_LEFT_4_SELF_ANY=4.00
1115
PRIMER_RIGHT_4_SELF_ANY=4.00
1116
PRIMER_LEFT_4_SELF_END=4.00
1117
PRIMER_RIGHT_4_SELF_END=3.00
1118
PRIMER_LEFT_4_END_STABILITY=5.6000
1119
PRIMER_RIGHT_4_END_STABILITY=6.7000
1120
PRIMER_PAIR_4_COMPL_ANY=4.00
1121
PRIMER_PAIR_4_COMPL_END=2.00
1122
PRIMER_PRODUCT_SIZE_4=131
1123
=
1124
MARKER_NAME=13.RHAP34FB1.seq
1125
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNGAGAGGTGTATATAGAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGTATCCAAAACTCTTTAGTCTTAATGTATTTATATACCTTTATTGTTATAAAATTCCTGTTTAAAACACAGTCTGAACAAGGTGACTTCTTTTAGAATTTGTCAGTAAGGAACACATTTTTTCTTTACCTATGTCTTTTTCTTAGACTGGGTCAGCACAATATTTGTAACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
1126
INCLUDED_REGION=21,244
1127
TARGET=22,16,TA
1128
TARGET=57,34,GT
1129
PRIMER_COMMENT=CL is
1130
PRIMER_COMMENT=CR is
1131
=
1132
MARKER_NAME=14.RHAP34FB2.seq
1133
SEQUENCE=NNNNNNNNNNNNNACTCATATACATAAAATAAATAGATATTTTTAAATAAACAGAAGATTGCAACGAAATCAAGCAGAATTCAAGACAATGAATAAATAACAAAATCTAAAAGGCTCAAGACTGCAACACTCCTGTGGGAGCACACATGTGTGCATGGAACACNCAAATGCGCNCNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTNCACACAANNCTACNGCNNCACNNTTAGCACNCGCACAAGNNNNNNNNNNNNNNTTCNGCNACNACATNNNNANNNCNCNAANACNNCNACNNCNCNNTNNC
1134
INCLUDED_REGION=13,303
1135
TARGET=177,33,AC
1136
TARGET=254,14,AC
1137
PRIMER_COMMENT=CL is
1138
PRIMER_COMMENT=CR is
1139
=
1140
MARKER_NAME=14.RHAP34FF2.seq
1141
SEQUENCE=NNNNNNNNNNNNNNNNNNNNCCATTTGGAAAGTCAACCTTCAAGGCGTGTGTTCTTCCTCCTTGGCACCTTGAGGAGGCAGCAATGACATTTTGACAAATGCCTCAAGTTAGGTAGGTGCTCTATAGCAAAGAGCCAATACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGCGTGTACGAATGTGTGTAATGAAAGGGGAATATTCTGGCTTAGCTTACACATTAAGTTCATTCAAGGTCATTCAATAGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNN
1142
INCLUDED_REGION=20,248
1143
TARGET=141,44,TG
1144
PRIMER_COMMENT=CL is GGAGGCAGCAATGACATTTT
1145
PRIMER_COMMENT=CR is TACACACATTCGTACACGCG
1146
PRIMER_PAIR_PENALTY=0.8553
1147
PRIMER_LEFT_PENALTY=0.080962
1148
PRIMER_RIGHT_PENALTY=0.774354
1149
PRIMER_LEFT_SEQUENCE=GGAGGCAGCAATGACATTTT
1150
PRIMER_RIGHT_SEQUENCE=TACACACATTCGTACACGCG
1151
PRIMER_LEFT=73,20
1152
PRIMER_RIGHT=204,20
1153
PRIMER_LEFT_TM=60.081
1154
PRIMER_RIGHT_TM=59.226
1155
PRIMER_LEFT_GC_PERCENT=45.000
1156
PRIMER_RIGHT_GC_PERCENT=50.000
1157
PRIMER_LEFT_SELF_ANY=7.00
1158
PRIMER_RIGHT_SELF_ANY=4.00
1159
PRIMER_LEFT_SELF_END=3.00
1160
PRIMER_RIGHT_SELF_END=4.00
1161
PRIMER_LEFT_END_STABILITY=7.2000
1162
PRIMER_RIGHT_END_STABILITY=11.6000
1163
PRIMER_PAIR_COMPL_ANY=4.00
1164
PRIMER_PAIR_COMPL_END=0.00
1165
PRIMER_PRODUCT_SIZE=132
1166
PRIMER_PAIR_PENALTY_1=0.9306
1167
PRIMER_LEFT_1_PENALTY=0.156287
1168
PRIMER_RIGHT_1_PENALTY=0.774354
1169
PRIMER_LEFT_1_SEQUENCE=AACCTTCAAGGCGTGTGTTC
1170
PRIMER_RIGHT_1_SEQUENCE=TACACACATTCGTACACGCG
1171
PRIMER_LEFT_1=34,20
1172
PRIMER_RIGHT_1=204,20
1173
PRIMER_LEFT_1_TM=60.156
1174
PRIMER_RIGHT_1_TM=59.226
1175
PRIMER_LEFT_1_GC_PERCENT=50.000
1176
PRIMER_RIGHT_1_GC_PERCENT=50.000
1177
PRIMER_LEFT_1_SELF_ANY=7.00
1178
PRIMER_RIGHT_1_SELF_ANY=4.00
1179
PRIMER_LEFT_1_SELF_END=0.00
1180
PRIMER_RIGHT_1_SELF_END=4.00
1181
PRIMER_LEFT_1_END_STABILITY=6.7000
1182
PRIMER_RIGHT_1_END_STABILITY=11.6000
1183
PRIMER_PAIR_1_COMPL_ANY=6.00
1184
PRIMER_PAIR_1_COMPL_END=5.00
1185
PRIMER_PRODUCT_SIZE_1=171
1186
PRIMER_PAIR_PENALTY_2=1.0056
1187
PRIMER_LEFT_2_PENALTY=0.231272
1188
PRIMER_RIGHT_2_PENALTY=0.774354
1189
PRIMER_LEFT_2_SEQUENCE=ACCTTCAAGGCGTGTGTTCT
1190
PRIMER_RIGHT_2_SEQUENCE=TACACACATTCGTACACGCG
1191
PRIMER_LEFT_2=35,20
1192
PRIMER_RIGHT_2=204,20
1193
PRIMER_LEFT_2_TM=59.769
1194
PRIMER_RIGHT_2_TM=59.226
1195
PRIMER_LEFT_2_GC_PERCENT=50.000
1196
PRIMER_RIGHT_2_GC_PERCENT=50.000
1197
PRIMER_LEFT_2_SELF_ANY=7.00
1198
PRIMER_RIGHT_2_SELF_ANY=4.00
1199
PRIMER_LEFT_2_SELF_END=0.00
1200
PRIMER_RIGHT_2_SELF_END=4.00
1201
PRIMER_LEFT_2_END_STABILITY=6.4000
1202
PRIMER_RIGHT_2_END_STABILITY=11.6000
1203
PRIMER_PAIR_2_COMPL_ANY=6.00
1204
PRIMER_PAIR_2_COMPL_END=4.00
1205
PRIMER_PRODUCT_SIZE_2=170
1206
PRIMER_PAIR_PENALTY_3=1.0056
1207
PRIMER_LEFT_3_PENALTY=0.231272
1208
PRIMER_RIGHT_3_PENALTY=0.774354
1209
PRIMER_LEFT_3_SEQUENCE=AGTCAACCTTCAAGGCGTGT
1210
PRIMER_RIGHT_3_SEQUENCE=TACACACATTCGTACACGCG
1211
PRIMER_LEFT_3=30,20
1212
PRIMER_RIGHT_3=204,20
1213
PRIMER_LEFT_3_TM=59.769
1214
PRIMER_RIGHT_3_TM=59.226
1215
PRIMER_LEFT_3_GC_PERCENT=50.000
1216
PRIMER_RIGHT_3_GC_PERCENT=50.000
1217
PRIMER_LEFT_3_SELF_ANY=7.00
1218
PRIMER_RIGHT_3_SELF_ANY=4.00
1219
PRIMER_LEFT_3_SELF_END=3.00
1220
PRIMER_RIGHT_3_SELF_END=4.00
1221
PRIMER_LEFT_3_END_STABILITY=8.1000
1222
PRIMER_RIGHT_3_END_STABILITY=11.6000
1223
PRIMER_PAIR_3_COMPL_ANY=6.00
1224
PRIMER_PAIR_3_COMPL_END=5.00
1225
PRIMER_PRODUCT_SIZE_3=175
1226
PRIMER_PAIR_PENALTY_4=1.0649
1227
PRIMER_LEFT_4_PENALTY=0.290589
1228
PRIMER_RIGHT_4_PENALTY=0.774354
1229
PRIMER_LEFT_4_SEQUENCE=CCTTCAAGGCGTGTGTTCTT
1230
PRIMER_RIGHT_4_SEQUENCE=TACACACATTCGTACACGCG
1231
PRIMER_LEFT_4=36,20
1232
PRIMER_RIGHT_4=204,20
1233
PRIMER_LEFT_4_TM=60.291
1234
PRIMER_RIGHT_4_TM=59.226
1235
PRIMER_LEFT_4_GC_PERCENT=50.000
1236
PRIMER_RIGHT_4_GC_PERCENT=50.000
1237
PRIMER_LEFT_4_SELF_ANY=7.00
1238
PRIMER_RIGHT_4_SELF_ANY=4.00
1239
PRIMER_LEFT_4_SELF_END=0.00
1240
PRIMER_RIGHT_4_SELF_END=4.00
1241
PRIMER_LEFT_4_END_STABILITY=7.0000
1242
PRIMER_RIGHT_4_END_STABILITY=11.6000
1243
PRIMER_PAIR_4_COMPL_ANY=6.00
1244
PRIMER_PAIR_4_COMPL_END=3.00
1245
PRIMER_PRODUCT_SIZE_4=169
1246
=
1247
MARKER_NAME=15.RHAP34FF3.seq
1248
SEQUENCE=NNNNNNNNCACTGCCTGTCTCTGCTCTTTACTGAGAACAGTCAAGGGGACAGTATTGTTATCTCCACATATTTCACACTTTTCTTAACCCTTTCCTTAAGATTTTATCTCCTCCATTTTNNNNNNNNNNNNNNNNNNNNNNNNGCGCGCGGCCCNNNNNNNNNNNNNNNNNNNNNNNNNCTTTTGCTGGGCTCTTAAGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
1249
INCLUDED_REGION=8,193
1250
TARGET=119,24,AC
1251
TARGET=154,25,CA
1252
PRIMER_COMMENT=CL is TGAGAACAGTCAAGGGGACA
1253
PRIMER_COMMENT=CR is CCACTTAAGAGCCCAGCAAA
1254
PRIMER_PAIR_PENALTY=1.1481
1255
PRIMER_LEFT_PENALTY=0.770184
1256
PRIMER_RIGHT_PENALTY=0.377925
1257
PRIMER_LEFT_SEQUENCE=TGAGAACAGTCAAGGGGACA
1258
PRIMER_RIGHT_SEQUENCE=CCACTTAAGAGCCCAGCAAA
1259
PRIMER_LEFT=31,20
1260
PRIMER_RIGHT=200,20
1261
PRIMER_LEFT_TM=59.230
1262
PRIMER_RIGHT_TM=60.378
1263
PRIMER_LEFT_GC_PERCENT=50.000
1264
PRIMER_RIGHT_GC_PERCENT=50.000
1265
PRIMER_LEFT_SELF_ANY=3.00
1266
PRIMER_RIGHT_SELF_ANY=6.00
1267
PRIMER_LEFT_SELF_END=0.00
1268
PRIMER_RIGHT_SELF_END=0.00
1269
PRIMER_LEFT_END_STABILITY=7.9000
1270
PRIMER_RIGHT_END_STABILITY=8.8000
1271
PRIMER_PAIR_COMPL_ANY=4.00
1272
PRIMER_PAIR_COMPL_END=0.00
1273
PRIMER_PRODUCT_SIZE=170
1274
PRIMER_PAIR_PENALTY_1=1.6603
1275
PRIMER_LEFT_1_PENALTY=1.282340
1276
PRIMER_RIGHT_1_PENALTY=0.377925
1277
PRIMER_LEFT_1_SEQUENCE=CTGAGAACAGTCAAGGGGACA
1278
PRIMER_RIGHT_1_SEQUENCE=CCACTTAAGAGCCCAGCAAA
1279
PRIMER_LEFT_1=30,21
1280
PRIMER_RIGHT_1=200,20
1281
PRIMER_LEFT_1_TM=60.282
1282
PRIMER_RIGHT_1_TM=60.378
1283
PRIMER_LEFT_1_GC_PERCENT=52.381
1284
PRIMER_RIGHT_1_GC_PERCENT=50.000
1285
PRIMER_LEFT_1_SELF_ANY=3.00
1286
PRIMER_RIGHT_1_SELF_ANY=6.00
1287
PRIMER_LEFT_1_SELF_END=0.00
1288
PRIMER_RIGHT_1_SELF_END=0.00
1289
PRIMER_LEFT_1_END_STABILITY=7.9000
1290
PRIMER_RIGHT_1_END_STABILITY=8.8000
1291
PRIMER_PAIR_1_COMPL_ANY=4.00
1292
PRIMER_PAIR_1_COMPL_END=0.00
1293
PRIMER_PRODUCT_SIZE_1=171
1294
PRIMER_PAIR_PENALTY_2=1.6603
1295
PRIMER_LEFT_2_PENALTY=1.282340
1296
PRIMER_RIGHT_2_PENALTY=0.377925
1297
PRIMER_LEFT_2_SEQUENCE=TGAGAACAGTCAAGGGGACAG
1298
PRIMER_RIGHT_2_SEQUENCE=CCACTTAAGAGCCCAGCAAA
1299
PRIMER_LEFT_2=31,21
1300
PRIMER_RIGHT_2=200,20
1301
PRIMER_LEFT_2_TM=60.282
1302
PRIMER_RIGHT_2_TM=60.378
1303
PRIMER_LEFT_2_GC_PERCENT=52.381
1304
PRIMER_RIGHT_2_GC_PERCENT=50.000
1305
PRIMER_LEFT_2_SELF_ANY=3.00
1306
PRIMER_RIGHT_2_SELF_ANY=6.00
1307
PRIMER_LEFT_2_SELF_END=1.00
1308
PRIMER_RIGHT_2_SELF_END=0.00
1309
PRIMER_LEFT_2_END_STABILITY=6.4000
1310
PRIMER_RIGHT_2_END_STABILITY=8.8000
1311
PRIMER_PAIR_2_COMPL_ANY=4.00
1312
PRIMER_PAIR_2_COMPL_END=0.00
1313
PRIMER_PRODUCT_SIZE_2=170
1314
PRIMER_PAIR_PENALTY_3=2.5962
1315
PRIMER_LEFT_3_PENALTY=0.770184
1316
PRIMER_RIGHT_3_PENALTY=1.825970
1317
PRIMER_LEFT_3_SEQUENCE=TGAGAACAGTCAAGGGGACA
1318
PRIMER_RIGHT_3_SEQUENCE=CACTTAAGAGCCCAGCAAAA
1319
PRIMER_LEFT_3=31,20
1320
PRIMER_RIGHT_3=199,20
1321
PRIMER_LEFT_3_TM=59.230
1322
PRIMER_RIGHT_3_TM=58.174
1323
PRIMER_LEFT_3_GC_PERCENT=50.000
1324
PRIMER_RIGHT_3_GC_PERCENT=45.000
1325
PRIMER_LEFT_3_SELF_ANY=3.00
1326
PRIMER_RIGHT_3_SELF_ANY=6.00
1327
PRIMER_LEFT_3_SELF_END=0.00
1328
PRIMER_RIGHT_3_SELF_END=0.00
1329
PRIMER_LEFT_3_END_STABILITY=7.9000
1330
PRIMER_RIGHT_3_END_STABILITY=7.6000
1331
PRIMER_PAIR_3_COMPL_ANY=3.00
1332
PRIMER_PAIR_3_COMPL_END=0.00
1333
PRIMER_PRODUCT_SIZE_3=169
1334
PRIMER_PAIR_PENALTY_4=2.6063
1335
PRIMER_LEFT_4_PENALTY=0.770184
1336
PRIMER_RIGHT_4_PENALTY=1.836139
1337
PRIMER_LEFT_4_SEQUENCE=TGAGAACAGTCAAGGGGACA
1338
PRIMER_RIGHT_4_SEQUENCE=CACTTAAGAGCCCAGCAAAAG
1339
PRIMER_LEFT_4=31,20
1340
PRIMER_RIGHT_4=199,21
1341
PRIMER_LEFT_4_TM=59.230
1342
PRIMER_RIGHT_4_TM=59.164
1343
PRIMER_LEFT_4_GC_PERCENT=50.000
1344
PRIMER_RIGHT_4_GC_PERCENT=47.619
1345
PRIMER_LEFT_4_SELF_ANY=3.00
1346
PRIMER_RIGHT_4_SELF_ANY=6.00
1347
PRIMER_LEFT_4_SELF_END=0.00
1348
PRIMER_RIGHT_4_SELF_END=0.00
1349
PRIMER_LEFT_4_END_STABILITY=7.9000
1350
PRIMER_RIGHT_4_END_STABILITY=7.3000
1351
PRIMER_PAIR_4_COMPL_ANY=3.00
1352
PRIMER_PAIR_4_COMPL_END=0.00
1353
PRIMER_PRODUCT_SIZE_4=169
1354
=
1355
MARKER_NAME=16.RHAP33FF4.seq
1356
SEQUENCE=NNNNNNNNNNNNNNNNNCTTTGGACTCTGATGGAAGAACAGAATGAAAAGGATTATGGCCTCTAAGGGCAAAGGTACTCCACAGGCTTTGTCTTCATGTGGGTGTCGAACAACTAGAATTGGGCTTTCCCAAAACATGTTGTCTGTAGATGGGATATCTGTGTCTTTAAAATTCATTTATTTATTTACTCACTTTATATCCCAAAACTGGTGCTCCATCTTCATGGGATACCAACACCCATTGTTAGGGGAACCCTTAAAAAAACAAAGCGTGCGCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGGGGGGGGCAATGGGGGGTGAGGAGNCAGGAGGAGNNNNCNNAGGACAGAGGACCGAGT
1357
INCLUDED_REGION=17,363
1358
TARGET=278,41,AC
1359
PRIMER_COMMENT=CL is
1360
PRIMER_COMMENT=CR is
1361
=
1362
MARKER_NAME=16.RHAP34FF4.seq
1363
SEQUENCE=NNNNNNNNNNNNNNNNNNNNCAGAAAACTCCGGAAGAGACCACCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAACCCATTCACGTGGGAGAGGGAGTGGACGGGCTCATTCTTGGATCTGCGGAGGGGTGGTTCGTAGGCACCTGGGAAGGGACTCTCCCATTTCTTACTGGTGTGGTGGGGTCTTGAAAGGAAGCTGCCCAAGCTGGGCGCTGGGCGGATGGCTCACGCCTTTAATTCCCCCGCACTGGGGGGGTAGCCAGTAGGAGAGATGGACCACCGANTTC
1364
INCLUDED_REGION=20,279
1365
TARGET=46,38,TG
1366
PRIMER_COMMENT=CL is AACTCCGGAAGAGACCACCT
1367
PRIMER_COMMENT=CR is GAAATGGGAGAGTCCCTTCC
1368
PRIMER_PAIR_PENALTY=0.2370
1369
PRIMER_LEFT_PENALTY=0.110797
1370
PRIMER_RIGHT_PENALTY=0.126193
1371
PRIMER_LEFT_SEQUENCE=AACTCCGGAAGAGACCACCT
1372
PRIMER_RIGHT_SEQUENCE=GAAATGGGAGAGTCCCTTCC
1373
PRIMER_LEFT=25,20
1374
PRIMER_RIGHT=177,20
1375
PRIMER_LEFT_TM=60.111
1376
PRIMER_RIGHT_TM=59.874
1377
PRIMER_LEFT_GC_PERCENT=55.000
1378
PRIMER_RIGHT_GC_PERCENT=55.000
1379
PRIMER_LEFT_SELF_ANY=6.00
1380
PRIMER_RIGHT_SELF_ANY=5.00
1381
PRIMER_LEFT_SELF_END=0.00
1382
PRIMER_RIGHT_SELF_END=5.00
1383
PRIMER_LEFT_END_STABILITY=7.9000
1384
PRIMER_RIGHT_END_STABILITY=8.2000
1385
PRIMER_PAIR_COMPL_ANY=7.00
1386
PRIMER_PAIR_COMPL_END=6.00
1387
PRIMER_PRODUCT_SIZE=153
1388
PRIMER_PAIR_PENALTY_1=0.3860
1389
PRIMER_LEFT_1_PENALTY=0.110797
1390
PRIMER_RIGHT_1_PENALTY=0.275194
1391
PRIMER_LEFT_1_SEQUENCE=AACTCCGGAAGAGACCACCT
1392
PRIMER_RIGHT_1_SEQUENCE=ACCCCACCACACCAGTAAGA
1393
PRIMER_LEFT_1=25,20
1394
PRIMER_RIGHT_1=195,20
1395
PRIMER_LEFT_1_TM=60.111
1396
PRIMER_RIGHT_1_TM=60.275
1397
PRIMER_LEFT_1_GC_PERCENT=55.000
1398
PRIMER_RIGHT_1_GC_PERCENT=55.000
1399
PRIMER_LEFT_1_SELF_ANY=6.00
1400
PRIMER_RIGHT_1_SELF_ANY=2.00
1401
PRIMER_LEFT_1_SELF_END=0.00
1402
PRIMER_RIGHT_1_SELF_END=0.00
1403
PRIMER_LEFT_1_END_STABILITY=7.9000
1404
PRIMER_RIGHT_1_END_STABILITY=6.0000
1405
PRIMER_PAIR_1_COMPL_ANY=3.00
1406
PRIMER_PAIR_1_COMPL_END=1.00
1407
PRIMER_PRODUCT_SIZE_1=171
1408
PRIMER_PAIR_PENALTY_2=0.3860
1409
PRIMER_LEFT_2_PENALTY=0.110797
1410
PRIMER_RIGHT_2_PENALTY=0.275194
1411
PRIMER_LEFT_2_SEQUENCE=AACTCCGGAAGAGACCACCT
1412
PRIMER_RIGHT_2_SEQUENCE=GACCCCACCACACCAGTAAG
1413
PRIMER_LEFT_2=25,20
1414
PRIMER_RIGHT_2=196,20
1415
PRIMER_LEFT_2_TM=60.111
1416
PRIMER_RIGHT_2_TM=60.275
1417
PRIMER_LEFT_2_GC_PERCENT=55.000
1418
PRIMER_RIGHT_2_GC_PERCENT=60.000
1419
PRIMER_LEFT_2_SELF_ANY=6.00
1420
PRIMER_RIGHT_2_SELF_ANY=2.00
1421
PRIMER_LEFT_2_SELF_END=0.00
1422
PRIMER_RIGHT_2_SELF_END=0.00
1423
PRIMER_LEFT_2_END_STABILITY=7.9000
1424
PRIMER_RIGHT_2_END_STABILITY=5.7000
1425
PRIMER_PAIR_2_COMPL_ANY=3.00
1426
PRIMER_PAIR_2_COMPL_END=2.00
1427
PRIMER_PRODUCT_SIZE_2=172
1428
PRIMER_PAIR_PENALTY_3=0.3860
1429
PRIMER_LEFT_3_PENALTY=0.110797
1430
PRIMER_RIGHT_3_PENALTY=0.275194
1431
PRIMER_LEFT_3_SEQUENCE=AACTCCGGAAGAGACCACCT
1432
PRIMER_RIGHT_3_SEQUENCE=AGACCCCACCACACCAGTAA
1433
PRIMER_LEFT_3=25,20
1434
PRIMER_RIGHT_3=197,20
1435
PRIMER_LEFT_3_TM=60.111
1436
PRIMER_RIGHT_3_TM=60.275
1437
PRIMER_LEFT_3_GC_PERCENT=55.000
1438
PRIMER_RIGHT_3_GC_PERCENT=55.000
1439
PRIMER_LEFT_3_SELF_ANY=6.00
1440
PRIMER_RIGHT_3_SELF_ANY=2.00
1441
PRIMER_LEFT_3_SELF_END=0.00
1442
PRIMER_RIGHT_3_SELF_END=1.00
1443
PRIMER_LEFT_3_END_STABILITY=7.9000
1444
PRIMER_RIGHT_3_END_STABILITY=5.7000
1445
PRIMER_PAIR_3_COMPL_ANY=3.00
1446
PRIMER_PAIR_3_COMPL_END=1.00
1447
PRIMER_PRODUCT_SIZE_3=173
1448
PRIMER_PAIR_PENALTY_4=0.3860
1449
PRIMER_LEFT_4_PENALTY=0.110797
1450
PRIMER_RIGHT_4_PENALTY=0.275194
1451
PRIMER_LEFT_4_SEQUENCE=AACTCCGGAAGAGACCACCT
1452
PRIMER_RIGHT_4_SEQUENCE=AAGACCCCACCACACCAGTA
1453
PRIMER_LEFT_4=25,20
1454
PRIMER_RIGHT_4=198,20
1455
PRIMER_LEFT_4_TM=60.111
1456
PRIMER_RIGHT_4_TM=60.275
1457
PRIMER_LEFT_4_GC_PERCENT=55.000
1458
PRIMER_RIGHT_4_GC_PERCENT=55.000
1459
PRIMER_LEFT_4_SELF_ANY=6.00
1460
PRIMER_RIGHT_4_SELF_ANY=2.00
1461
PRIMER_LEFT_4_SELF_END=0.00
1462
PRIMER_RIGHT_4_SELF_END=2.00
1463
PRIMER_LEFT_4_END_STABILITY=7.9000
1464
PRIMER_RIGHT_4_END_STABILITY=5.7000
1465
PRIMER_PAIR_4_COMPL_ANY=3.00
1466
PRIMER_PAIR_4_COMPL_END=1.00
1467
PRIMER_PRODUCT_SIZE_4=174
1468
=
1469
MARKER_NAME=17.RHAP34FF5.seq
1470
SEQUENCE=NNNNNNNNNNNNNNNNNNCTTGACTTCTATGTATGCACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTACATCTTATACATCTCTATTGTGTCCTTGTCATGTGGAGGTCAGAGATGAATATACATCTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNTATACATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGGTTGTGGTGNACATCTCTCTCTGTTTATATTGTGGCTTGTCATGGGGAGGCCAAGAGGTTTAAGGACTCACACAGCTCT
1471
INCLUDED_REGION=18,285
1472
TARGET=40,50,TG
1473
TARGET=154,26,TG
1474
TARGET=188,33,TG
1475
PRIMER_COMMENT=CL is TCCTTGTCATGTGGAGGTCA
1476
PRIMER_COMMENT=CR is CTTAAACCTCTTGGCCTCCC
1477
PRIMER_PAIR_PENALTY=0.1555
1478
PRIMER_LEFT_PENALTY=0.087977
1479
PRIMER_RIGHT_PENALTY=0.067516
1480
PRIMER_LEFT_SEQUENCE=TCCTTGTCATGTGGAGGTCA
1481
PRIMER_RIGHT_SEQUENCE=CTTAAACCTCTTGGCCTCCC
1482
PRIMER_LEFT=114,20
1483
PRIMER_RIGHT=287,20
1484
PRIMER_LEFT_TM=60.088
1485
PRIMER_RIGHT_TM=60.068
1486
PRIMER_LEFT_GC_PERCENT=50.000
1487
PRIMER_RIGHT_GC_PERCENT=55.000
1488
PRIMER_LEFT_SELF_ANY=4.00
1489
PRIMER_RIGHT_SELF_ANY=4.00
1490
PRIMER_LEFT_SELF_END=1.00
1491
PRIMER_RIGHT_SELF_END=0.00
1492
PRIMER_LEFT_END_STABILITY=7.9000
1493
PRIMER_RIGHT_END_STABILITY=9.4000
1494
PRIMER_PAIR_COMPL_ANY=6.00
1495
PRIMER_PAIR_COMPL_END=5.00
1496
PRIMER_PRODUCT_SIZE=174
1497
PRIMER_PAIR_PENALTY_1=0.1555
1498
PRIMER_LEFT_1_PENALTY=0.087977
1499
PRIMER_RIGHT_1_PENALTY=0.067516
1500
PRIMER_LEFT_1_SEQUENCE=TCCTTGTCATGTGGAGGTCA
1501
PRIMER_RIGHT_1_SEQUENCE=CCTTAAACCTCTTGGCCTCC
1502
PRIMER_LEFT_1=114,20
1503
PRIMER_RIGHT_1=288,20
1504
PRIMER_LEFT_1_TM=60.088
1505
PRIMER_RIGHT_1_TM=60.068
1506
PRIMER_LEFT_1_GC_PERCENT=50.000
1507
PRIMER_RIGHT_1_GC_PERCENT=55.000
1508
PRIMER_LEFT_1_SELF_ANY=4.00
1509
PRIMER_RIGHT_1_SELF_ANY=4.00
1510
PRIMER_LEFT_1_SELF_END=1.00
1511
PRIMER_RIGHT_1_SELF_END=1.00
1512
PRIMER_LEFT_1_END_STABILITY=7.9000
1513
PRIMER_RIGHT_1_END_STABILITY=9.4000
1514
PRIMER_PAIR_1_COMPL_ANY=6.00
1515
PRIMER_PAIR_1_COMPL_END=6.00
1516
PRIMER_PRODUCT_SIZE_1=175
1517
PRIMER_PAIR_PENALTY_2=0.9702
1518
PRIMER_LEFT_2_PENALTY=0.902727
1519
PRIMER_RIGHT_2_PENALTY=0.067516
1520
PRIMER_LEFT_2_SEQUENCE=CCTTGTCATGTGGAGGTCAG
1521
PRIMER_RIGHT_2_SEQUENCE=CTTAAACCTCTTGGCCTCCC
1522
PRIMER_LEFT_2=115,20
1523
PRIMER_RIGHT_2=287,20
1524
PRIMER_LEFT_2_TM=59.097
1525
PRIMER_RIGHT_2_TM=60.068
1526
PRIMER_LEFT_2_GC_PERCENT=55.000
1527
PRIMER_RIGHT_2_GC_PERCENT=55.000
1528
PRIMER_LEFT_2_SELF_ANY=4.00
1529
PRIMER_RIGHT_2_SELF_ANY=4.00
1530
PRIMER_LEFT_2_SELF_END=1.00
1531
PRIMER_RIGHT_2_SELF_END=0.00
1532
PRIMER_LEFT_2_END_STABILITY=6.4000
1533
PRIMER_RIGHT_2_END_STABILITY=9.4000
1534
PRIMER_PAIR_2_COMPL_ANY=6.00
1535
PRIMER_PAIR_2_COMPL_END=4.00
1536
PRIMER_PRODUCT_SIZE_2=173
1537
PRIMER_PAIR_PENALTY_3=0.9702
1538
PRIMER_LEFT_3_PENALTY=0.902727
1539
PRIMER_RIGHT_3_PENALTY=0.067516
1540
PRIMER_LEFT_3_SEQUENCE=CCTTGTCATGTGGAGGTCAG
1541
PRIMER_RIGHT_3_SEQUENCE=CCTTAAACCTCTTGGCCTCC
1542
PRIMER_LEFT_3=115,20
1543
PRIMER_RIGHT_3=288,20
1544
PRIMER_LEFT_3_TM=59.097
1545
PRIMER_RIGHT_3_TM=60.068
1546
PRIMER_LEFT_3_GC_PERCENT=55.000
1547
PRIMER_RIGHT_3_GC_PERCENT=55.000
1548
PRIMER_LEFT_3_SELF_ANY=4.00
1549
PRIMER_RIGHT_3_SELF_ANY=4.00
1550
PRIMER_LEFT_3_SELF_END=1.00
1551
PRIMER_RIGHT_3_SELF_END=1.00
1552
PRIMER_LEFT_3_END_STABILITY=6.4000
1553
PRIMER_RIGHT_3_END_STABILITY=9.4000
1554
PRIMER_PAIR_3_COMPL_ANY=6.00
1555
PRIMER_PAIR_3_COMPL_END=5.00
1556
PRIMER_PRODUCT_SIZE_3=174
1557
PRIMER_PAIR_PENALTY_4=1.7810
1558
PRIMER_LEFT_4_PENALTY=0.087977
1559
PRIMER_RIGHT_4_PENALTY=1.693048
1560
PRIMER_LEFT_4_SEQUENCE=TCCTTGTCATGTGGAGGTCA
1561
PRIMER_RIGHT_4_SEQUENCE=CCCCATGACAAGCCACAATA
1562
PRIMER_LEFT_4=114,20
1563
PRIMER_RIGHT_4=270,20
1564
PRIMER_LEFT_4_TM=60.088
1565
PRIMER_RIGHT_4_TM=61.693
1566
PRIMER_LEFT_4_GC_PERCENT=50.000
1567
PRIMER_RIGHT_4_GC_PERCENT=50.000
1568
PRIMER_LEFT_4_SELF_ANY=4.00
1569
PRIMER_RIGHT_4_SELF_ANY=4.00
1570
PRIMER_LEFT_4_SELF_END=1.00
1571
PRIMER_RIGHT_4_SELF_END=2.00
1572
PRIMER_LEFT_4_END_STABILITY=7.9000
1573
PRIMER_RIGHT_4_END_STABILITY=6.2000
1574
PRIMER_PAIR_4_COMPL_ANY=10.00
1575
PRIMER_PAIR_4_COMPL_END=4.00
1576
PRIMER_PRODUCT_SIZE_4=157
1577
=
1578
MARKER_NAME=18.RHAP34FB6.seq
1579
SEQUENCE=NNNNNNNNNNNNNACATGGTTATATGTCAAAATAATTTAATTTGTTGGTTGTTTCTTCCCAAAAATTACTTTAGTAAGAGTCCAAAAACTATTATTAATTAATATTAAGTTTTNGGNGNTGGTGGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATGTGATGTGTGCGTGATGTGAGCATGTTCCATGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
1580
INCLUDED_REGION=13,197
1581
TARGET=127,46,GT
1582
PRIMER_COMMENT=CL is TGTTGGTTGTTTCTTCCCAA
1583
PRIMER_COMMENT=CR is CCATGGAACATGCTCACATC
1584
PRIMER_PAIR_PENALTY=1.0796
1585
PRIMER_LEFT_PENALTY=1.004672
1586
PRIMER_RIGHT_PENALTY=0.074917
1587
PRIMER_LEFT_SEQUENCE=TTGTTGGTTGTTTCTTCCCA
1588
PRIMER_RIGHT_SEQUENCE=CCATGGAACATGCTCACATC
1589
PRIMER_LEFT=41,20
1590
PRIMER_RIGHT=208,20
1591
PRIMER_LEFT_TM=58.995
1592
PRIMER_RIGHT_TM=59.925
1593
PRIMER_LEFT_GC_PERCENT=40.000
1594
PRIMER_RIGHT_GC_PERCENT=50.000
1595
PRIMER_LEFT_SELF_ANY=3.00
1596
PRIMER_RIGHT_SELF_ANY=8.00
1597
PRIMER_LEFT_SELF_END=0.00
1598
PRIMER_RIGHT_SELF_END=6.00
1599
PRIMER_LEFT_END_STABILITY=9.7000
1600
PRIMER_RIGHT_END_STABILITY=6.3000
1601
PRIMER_PAIR_COMPL_ANY=4.00
1602
PRIMER_PAIR_COMPL_END=1.00
1603
PRIMER_PRODUCT_SIZE=168
1604
PRIMER_PAIR_PENALTY_1=1.0796
1605
PRIMER_LEFT_1_PENALTY=1.004672
1606
PRIMER_RIGHT_1_PENALTY=0.074917
1607
PRIMER_LEFT_1_SEQUENCE=TGTTGGTTGTTTCTTCCCAA
1608
PRIMER_RIGHT_1_SEQUENCE=CCATGGAACATGCTCACATC
1609
PRIMER_LEFT_1=42,20
1610
PRIMER_RIGHT_1=208,20
1611
PRIMER_LEFT_1_TM=58.995
1612
PRIMER_RIGHT_1_TM=59.925
1613
PRIMER_LEFT_1_GC_PERCENT=40.000
1614
PRIMER_RIGHT_1_GC_PERCENT=50.000
1615
PRIMER_LEFT_1_SELF_ANY=4.00
1616
PRIMER_RIGHT_1_SELF_ANY=8.00
1617
PRIMER_LEFT_1_SELF_END=1.00
1618
PRIMER_RIGHT_1_SELF_END=6.00
1619
PRIMER_LEFT_1_END_STABILITY=10.0000
1620
PRIMER_RIGHT_1_END_STABILITY=6.3000
1621
PRIMER_PAIR_1_COMPL_ANY=4.00
1622
PRIMER_PAIR_1_COMPL_END=0.00
1623
PRIMER_PRODUCT_SIZE_1=167
1624
PRIMER_PAIR_PENALTY_2=1.1805
1625
PRIMER_LEFT_2_PENALTY=1.004672
1626
PRIMER_RIGHT_2_PENALTY=0.175780
1627
PRIMER_LEFT_2_SEQUENCE=TTGTTGGTTGTTTCTTCCCA
1628
PRIMER_RIGHT_2_SEQUENCE=TCACATCACGCACACATCAC
1629
PRIMER_LEFT_2=41,20
1630
PRIMER_RIGHT_2=195,20
1631
PRIMER_LEFT_2_TM=58.995
1632
PRIMER_RIGHT_2_TM=60.176
1633
PRIMER_LEFT_2_GC_PERCENT=40.000
1634
PRIMER_RIGHT_2_GC_PERCENT=50.000
1635
PRIMER_LEFT_2_SELF_ANY=3.00
1636
PRIMER_RIGHT_2_SELF_ANY=2.00
1637
PRIMER_LEFT_2_SELF_END=0.00
1638
PRIMER_RIGHT_2_SELF_END=0.00
1639
PRIMER_LEFT_2_END_STABILITY=9.7000
1640
PRIMER_RIGHT_2_END_STABILITY=6.3000
1641
PRIMER_PAIR_2_COMPL_ANY=3.00
1642
PRIMER_PAIR_2_COMPL_END=0.00
1643
PRIMER_PRODUCT_SIZE_2=155
1644
PRIMER_PAIR_PENALTY_3=1.1805
1645
PRIMER_LEFT_3_PENALTY=1.004672
1646
PRIMER_RIGHT_3_PENALTY=0.175780
1647
PRIMER_LEFT_3_SEQUENCE=TGTTGGTTGTTTCTTCCCAA
1648
PRIMER_RIGHT_3_SEQUENCE=TCACATCACGCACACATCAC
1649
PRIMER_LEFT_3=42,20
1650
PRIMER_RIGHT_3=195,20
1651
PRIMER_LEFT_3_TM=58.995
1652
PRIMER_RIGHT_3_TM=60.176
1653
PRIMER_LEFT_3_GC_PERCENT=40.000
1654
PRIMER_RIGHT_3_GC_PERCENT=50.000
1655
PRIMER_LEFT_3_SELF_ANY=4.00
1656
PRIMER_RIGHT_3_SELF_ANY=2.00
1657
PRIMER_LEFT_3_SELF_END=1.00
1658
PRIMER_RIGHT_3_SELF_END=0.00
1659
PRIMER_LEFT_3_END_STABILITY=10.0000
1660
PRIMER_RIGHT_3_END_STABILITY=6.3000
1661
PRIMER_PAIR_3_COMPL_ANY=3.00
1662
PRIMER_PAIR_3_COMPL_END=0.00
1663
PRIMER_PRODUCT_SIZE_3=154
1664
PRIMER_PAIR_PENALTY_4=1.3366
1665
PRIMER_LEFT_4_PENALTY=1.004672
1666
PRIMER_RIGHT_4_PENALTY=0.331890
1667
PRIMER_LEFT_4_SEQUENCE=TTGTTGGTTGTTTCTTCCCA
1668
PRIMER_RIGHT_4_SEQUENCE=CTCACATCACGCACACATCA
1669
PRIMER_LEFT_4=41,20
1670
PRIMER_RIGHT_4=196,20
1671
PRIMER_LEFT_4_TM=58.995
1672
PRIMER_RIGHT_4_TM=60.332
1673
PRIMER_LEFT_4_GC_PERCENT=40.000
1674
PRIMER_RIGHT_4_GC_PERCENT=50.000
1675
PRIMER_LEFT_4_SELF_ANY=3.00
1676
PRIMER_RIGHT_4_SELF_ANY=2.00
1677
PRIMER_LEFT_4_SELF_END=0.00
1678
PRIMER_RIGHT_4_SELF_END=1.00
1679
PRIMER_LEFT_4_END_STABILITY=9.7000
1680
PRIMER_RIGHT_4_END_STABILITY=6.9000
1681
PRIMER_PAIR_4_COMPL_ANY=3.00
1682
PRIMER_PAIR_4_COMPL_END=0.00
1683
PRIMER_PRODUCT_SIZE_4=156
1684
=
1685
MARKER_NAME=18.RHAP34FF6.seq
1686
SEQUENCE=NNNNNNNNNCCGTCTCAGTTAAAAGCAAATCTTGCCTCCATTCTGCGGGTTATTTTTGATGGTTTCCTCCTCATCTTCACTGGTCTGTTCTGGTGTTTGCTCCTAGGATTTGTCACGTGAATCCTGACTGATCCGACTTGCTTGTTTTTTTTTGCTCTTTCTTTAGTTCTCTATTTCTTTGCTCTTTTTCCTTGTACGGAAAGTTTTTTGCTTTCTCCTTTTTTCTTAAATGCACGCATGTGACTATTGTGATGGANNNNNNNNNNNNNNNNNNNNNNNNNATNNNN
1687
INCLUDED_REGION=9,274
1688
TARGET=256,25,TG
1689
PRIMER_COMMENT=CL is
1690
PRIMER_COMMENT=CR is
1691
=
1692
MARKER_NAME=19.RHAP34FB7.seq
1693
SEQUENCE=NNNNNNNNNNNNNNNNNNNTTTTCCACTTGTCTTGTTTCCAAACCTCCCTTTCCATCGAAAGCCATATAGTCATCTTTGTCCTATTATCAGCGNNNNNNNNNNNNNNNNNNNNANANANANGNGNGNGNGNGNANNNCNATCNGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGGGGGNANAGAGNAATATGTGNATATGNGNANATANGGGGAAANACACANANANGGGGGNAGNGNGCGCGNAAGNGNNGNNAGAANCANGGGANNAGNAGAGTGNNNNGNGGGGGNAG
1694
INCLUDED_REGION=19,286
1695
TARGET=93,20,CT
1696
TARGET=147,38,GT
1697
PRIMER_COMMENT=CL is
1698
PRIMER_COMMENT=CR is
1699
=
1700
MARKER_NAME=20.RHAP33FB8.seq
1701
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCTCTAGTAATCCTATTAGTTACACATGTAAATAACTAAGTGTAGCCCCTTAGGTGGAGACACCAGAGGGACAAGTGCATATTAGTTATTAGTAAACAAAAGAGCAAAAGGCTCTGAAGCTCTAGATCAACAAACAAAATCTATGGATGTAAAGAAATAATAAAGTCCAGGACAGAAGGAAATGAAACGTTGACAAAGTAATACCAATAATCAGTGAAACTAAAAGATGAGTGAGATTCACAGTTACTATTTGTAAGAGGTACATAGAATATTCGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTATAGTGCCTTAAGTACATATATATTTTATAGAAATGNATTACTTTAGG
1702
INCLUDED_REGION=23,356
1703
TARGET=299,30,TG
1704
PRIMER_COMMENT=CL is
1705
PRIMER_COMMENT=CR is
1706
=
1707
MARKER_NAME=20.RHAP34FB8.seq
1708
SEQUENCE=NNNNNNNNNNNNNNNNNNNACAAAGTGAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATACTGAGCTTCTAGAAGTAATATAGTTGCCAGAGANNANGGGGAAATATTGAGAACTTGCTAAATGTGTGCAGTCATAGTCAGACAAACAAAATAAGCTCTGGAGATCCATTACACAGATCGTGATTACAGCTCCCAATATTATTTCGAATAAGACACAGTAACTATACAAGGAAGCATTTGGATGTTCTTATACAGAAGAAATGATA
1709
INCLUDED_REGION=19,278
1710
TARGET=30,56,AC
1711
PRIMER_COMMENT=CL is
1712
PRIMER_COMMENT=CR is
1713
=
1714
MARKER_NAME=20.RHAP34FF8.seq
1715
SEQUENCE=NNNNNNNNNNNNNNNNNNNNCAAATGCCAGCATGCAAAACTATAAAAGGATAAAAATGAAAATAGAAATGAAGTGTTGGCAGGAGCAGAAAGTGATGGCGCAGAATAGGAGGGCATTTCACAATAGTCCATGTGGCAAGAACTGTAGCCACTGATACATTTCTTTTTTAGTTCCTCTTTACAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCCTTTAAAAATCTGCTTAAAAGAGCAGACAATATTAAGTTCCTG
1716
INCLUDED_REGION=20,279
1717
TARGET=184,70,AC
1718
PRIMER_COMMENT=CL is TGTGGCAAGAACTGTAGCCA
1719
PRIMER_COMMENT=CR is TGTCTGCTCTTTTAAGCAGATTTTT
1720
PRIMER_PAIR_PENALTY=5.4753
1721
PRIMER_LEFT_PENALTY=0.449441
1722
PRIMER_RIGHT_PENALTY=5.025871
1723
PRIMER_LEFT_SEQUENCE=TGTGGCAAGAACTGTAGCCA
1724
PRIMER_RIGHT_SEQUENCE=TGTCTGCTCTTTTAAGCAGATTTTT
1725
PRIMER_LEFT=130,20
1726
PRIMER_RIGHT=284,25
1727
PRIMER_LEFT_TM=60.449
1728
PRIMER_RIGHT_TM=59.974
1729
PRIMER_LEFT_GC_PERCENT=50.000
1730
PRIMER_RIGHT_GC_PERCENT=32.000
1731
PRIMER_LEFT_SELF_ANY=6.00
1732
PRIMER_RIGHT_SELF_ANY=7.00
1733
PRIMER_LEFT_SELF_END=6.00
1734
PRIMER_RIGHT_SELF_END=0.00
1735
PRIMER_LEFT_END_STABILITY=9.7000
1736
PRIMER_RIGHT_END_STABILITY=7.6000
1737
PRIMER_PAIR_COMPL_ANY=4.00
1738
PRIMER_PAIR_COMPL_END=2.00
1739
PRIMER_PRODUCT_SIZE=155
1740
PRIMER_PAIR_PENALTY_1=5.4753
1741
PRIMER_LEFT_1_PENALTY=0.449441
1742
PRIMER_RIGHT_1_PENALTY=5.025871
1743
PRIMER_LEFT_1_SEQUENCE=TGTGGCAAGAACTGTAGCCA
1744
PRIMER_RIGHT_1_SEQUENCE=TTGTCTGCTCTTTTAAGCAGATTTT
1745
PRIMER_LEFT_1=130,20
1746
PRIMER_RIGHT_1=285,25
1747
PRIMER_LEFT_1_TM=60.449
1748
PRIMER_RIGHT_1_TM=59.974
1749
PRIMER_LEFT_1_GC_PERCENT=50.000
1750
PRIMER_RIGHT_1_GC_PERCENT=32.000
1751
PRIMER_LEFT_1_SELF_ANY=6.00
1752
PRIMER_RIGHT_1_SELF_ANY=7.00
1753
PRIMER_LEFT_1_SELF_END=6.00
1754
PRIMER_RIGHT_1_SELF_END=1.00
1755
PRIMER_LEFT_1_END_STABILITY=9.7000
1756
PRIMER_RIGHT_1_END_STABILITY=7.2000
1757
PRIMER_PAIR_1_COMPL_ANY=4.00
1758
PRIMER_PAIR_1_COMPL_END=2.00
1759
PRIMER_PRODUCT_SIZE_1=156
1760
PRIMER_PAIR_PENALTY_2=5.6265
1761
PRIMER_LEFT_2_PENALTY=0.449441
1762
PRIMER_RIGHT_2_PENALTY=5.177016
1763
PRIMER_LEFT_2_SEQUENCE=TGTGGCAAGAACTGTAGCCA
1764
PRIMER_RIGHT_2_SEQUENCE=TGTCTGCTCTTTTAAGCAGATTTT
1765
PRIMER_LEFT_2=130,20
1766
PRIMER_RIGHT_2=284,24
1767
PRIMER_LEFT_2_TM=60.449
1768
PRIMER_RIGHT_2_TM=58.823
1769
PRIMER_LEFT_2_GC_PERCENT=50.000
1770
PRIMER_RIGHT_2_GC_PERCENT=33.333
1771
PRIMER_LEFT_2_SELF_ANY=6.00
1772
PRIMER_RIGHT_2_SELF_ANY=7.00
1773
PRIMER_LEFT_2_SELF_END=6.00
1774
PRIMER_RIGHT_2_SELF_END=1.00
1775
PRIMER_LEFT_2_END_STABILITY=9.7000
1776
PRIMER_RIGHT_2_END_STABILITY=7.2000
1777
PRIMER_PAIR_2_COMPL_ANY=4.00
1778
PRIMER_PAIR_2_COMPL_END=2.00
1779
PRIMER_PRODUCT_SIZE_2=155
1780
PRIMER_PAIR_PENALTY_3=5.6265
1781
PRIMER_LEFT_3_PENALTY=0.449441
1782
PRIMER_RIGHT_3_PENALTY=5.177016
1783
PRIMER_LEFT_3_SEQUENCE=TGTGGCAAGAACTGTAGCCA
1784
PRIMER_RIGHT_3_SEQUENCE=TTGTCTGCTCTTTTAAGCAGATTT
1785
PRIMER_LEFT_3=130,20
1786
PRIMER_RIGHT_3=285,24
1787
PRIMER_LEFT_3_TM=60.449
1788
PRIMER_RIGHT_3_TM=58.823
1789
PRIMER_LEFT_3_GC_PERCENT=50.000
1790
PRIMER_RIGHT_3_GC_PERCENT=33.333
1791
PRIMER_LEFT_3_SELF_ANY=6.00
1792
PRIMER_RIGHT_3_SELF_ANY=7.00
1793
PRIMER_LEFT_3_SELF_END=6.00
1794
PRIMER_RIGHT_3_SELF_END=2.00
1795
PRIMER_LEFT_3_END_STABILITY=9.7000
1796
PRIMER_RIGHT_3_END_STABILITY=6.9000
1797
PRIMER_PAIR_3_COMPL_ANY=4.00
1798
PRIMER_PAIR_3_COMPL_END=2.00
1799
PRIMER_PRODUCT_SIZE_3=156
1800
PRIMER_PAIR_PENALTY_4=5.8914
1801
PRIMER_LEFT_4_PENALTY=0.449441
1802
PRIMER_RIGHT_4_PENALTY=5.441933
1803
PRIMER_LEFT_4_SEQUENCE=TGTGGCAAGAACTGTAGCCA
1804
PRIMER_RIGHT_4_SEQUENCE=TGTCTGCTCTTTTAAGCAGATTT
1805
PRIMER_LEFT_4=130,20
1806
PRIMER_RIGHT_4=284,23
1807
PRIMER_LEFT_4_TM=60.449
1808
PRIMER_RIGHT_4_TM=57.558
1809
PRIMER_LEFT_4_GC_PERCENT=50.000
1810
PRIMER_RIGHT_4_GC_PERCENT=34.783
1811
PRIMER_LEFT_4_SELF_ANY=6.00
1812
PRIMER_RIGHT_4_SELF_ANY=7.00
1813
PRIMER_LEFT_4_SELF_END=6.00
1814
PRIMER_RIGHT_4_SELF_END=2.00
1815
PRIMER_LEFT_4_END_STABILITY=9.7000
1816
PRIMER_RIGHT_4_END_STABILITY=6.9000
1817
PRIMER_PAIR_4_COMPL_ANY=4.00
1818
PRIMER_PAIR_4_COMPL_END=2.00
1819
PRIMER_PRODUCT_SIZE_4=155
1820
=
1821
MARKER_NAME=21.RHAP33FB9.seq
1822
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCTCTTCATCCAGTCACCAACACGGGGCAGACTCAGGCTCATTGAGAAGCCTGCAGGTACAGAATGGCCCTGTTCCCTCTTACTAAATTTTGCTTGCAGTCAAGTTGCATAGCATGGAGTCCTGGTTGCAGGAATATCTCTATAGAGTTCTCAGATGCCCCCTCTCAGTGTTTCCATAAAACCTGGCTACCACGTGATGGAAGAAACAGCCTGATGTTACCATTTCCTTATTTTCACATGTATCTGCATGCATANNNNNNNNNNNNNNNNNNNNNNNNCTCTTGCTTGTAGAAGCTCACCAAAGCCATGCTTATACTACAGCCTGGTCTATTTCACATCCTTCAAGAGGTATACACAC
1823
INCLUDED_REGION=21,357
1824
TARGET=274,24,TG
1825
PRIMER_COMMENT=CL is AGTTCTCAGATGCCCCCTCT
1826
PRIMER_COMMENT=CR is CATGGCTTTGGTGAGCTTCT
1827
PRIMER_PAIR_PENALTY=0.6131
1828
PRIMER_LEFT_PENALTY=0.216148
1829
PRIMER_RIGHT_PENALTY=0.396944
1830
PRIMER_LEFT_SEQUENCE=AGAGTTCTCAGATGCCCCCT
1831
PRIMER_RIGHT_SEQUENCE=CATGGCTTTGGTGAGCTTCT
1832
PRIMER_LEFT=163,20
1833
PRIMER_RIGHT=328,20
1834
PRIMER_LEFT_TM=60.216
1835
PRIMER_RIGHT_TM=60.397
1836
PRIMER_LEFT_GC_PERCENT=55.000
1837
PRIMER_RIGHT_GC_PERCENT=50.000
1838
PRIMER_LEFT_SELF_ANY=5.00
1839
PRIMER_RIGHT_SELF_ANY=4.00
1840
PRIMER_LEFT_SELF_END=1.00
1841
PRIMER_RIGHT_SELF_END=1.00
1842
PRIMER_LEFT_END_STABILITY=10.9000
1843
PRIMER_RIGHT_END_STABILITY=6.7000
1844
PRIMER_PAIR_COMPL_ANY=4.00
1845
PRIMER_PAIR_COMPL_END=3.00
1846
PRIMER_PRODUCT_SIZE=166
1847
PRIMER_PAIR_PENALTY_1=0.6131
1848
PRIMER_LEFT_1_PENALTY=0.216148
1849
PRIMER_RIGHT_1_PENALTY=0.396944
1850
PRIMER_LEFT_1_SEQUENCE=AGTTCTCAGATGCCCCCTCT
1851
PRIMER_RIGHT_1_SEQUENCE=CATGGCTTTGGTGAGCTTCT
1852
PRIMER_LEFT_1=165,20
1853
PRIMER_RIGHT_1=328,20
1854
PRIMER_LEFT_1_TM=60.216
1855
PRIMER_RIGHT_1_TM=60.397
1856
PRIMER_LEFT_1_GC_PERCENT=55.000
1857
PRIMER_RIGHT_1_GC_PERCENT=50.000
1858
PRIMER_LEFT_1_SELF_ANY=5.00
1859
PRIMER_RIGHT_1_SELF_ANY=4.00
1860
PRIMER_LEFT_1_SELF_END=3.00
1861
PRIMER_RIGHT_1_SELF_END=1.00
1862
PRIMER_LEFT_1_END_STABILITY=7.9000
1863
PRIMER_RIGHT_1_END_STABILITY=6.7000
1864
PRIMER_PAIR_1_COMPL_ANY=4.00
1865
PRIMER_PAIR_1_COMPL_END=2.00
1866
PRIMER_PRODUCT_SIZE_1=164
1867
PRIMER_PAIR_PENALTY_2=0.9194
1868
PRIMER_LEFT_2_PENALTY=0.522443
1869
PRIMER_RIGHT_2_PENALTY=0.396944
1870
PRIMER_LEFT_2_SEQUENCE=AAACCTGGCTACCACGTGAT
1871
PRIMER_RIGHT_2_SEQUENCE=CATGGCTTTGGTGAGCTTCT
1872
PRIMER_LEFT_2=198,20
1873
PRIMER_RIGHT_2=328,20
1874
PRIMER_LEFT_2_TM=59.478
1875
PRIMER_RIGHT_2_TM=60.397
1876
PRIMER_LEFT_2_GC_PERCENT=50.000
1877
PRIMER_RIGHT_2_GC_PERCENT=50.000
1878
PRIMER_LEFT_2_SELF_ANY=6.00
1879
PRIMER_RIGHT_2_SELF_ANY=4.00
1880
PRIMER_LEFT_2_SELF_END=6.00
1881
PRIMER_RIGHT_2_SELF_END=1.00
1882
PRIMER_LEFT_2_END_STABILITY=6.3000
1883
PRIMER_RIGHT_2_END_STABILITY=6.7000
1884
PRIMER_PAIR_2_COMPL_ANY=5.00
1885
PRIMER_PAIR_2_COMPL_END=0.00
1886
PRIMER_PRODUCT_SIZE_2=131
1887
PRIMER_PAIR_PENALTY_3=0.9470
1888
PRIMER_LEFT_3_PENALTY=0.550092
1889
PRIMER_RIGHT_3_PENALTY=0.396944
1890
PRIMER_LEFT_3_SEQUENCE=AAAACCTGGCTACCACGTGA
1891
PRIMER_RIGHT_3_SEQUENCE=CATGGCTTTGGTGAGCTTCT
1892
PRIMER_LEFT_3=197,20
1893
PRIMER_RIGHT_3=328,20
1894
PRIMER_LEFT_3_TM=60.550
1895
PRIMER_RIGHT_3_TM=60.397
1896
PRIMER_LEFT_3_GC_PERCENT=50.000
1897
PRIMER_RIGHT_3_GC_PERCENT=50.000
1898
PRIMER_LEFT_3_SELF_ANY=6.00
1899
PRIMER_RIGHT_3_SELF_ANY=4.00
1900
PRIMER_LEFT_3_SELF_END=4.00
1901
PRIMER_RIGHT_3_SELF_END=1.00
1902
PRIMER_LEFT_3_END_STABILITY=8.4000
1903
PRIMER_RIGHT_3_END_STABILITY=6.7000
1904
PRIMER_PAIR_3_COMPL_ANY=5.00
1905
PRIMER_PAIR_3_COMPL_END=1.00
1906
PRIMER_PRODUCT_SIZE_3=132
1907
PRIMER_PAIR_PENALTY_4=1.1584
1908
PRIMER_LEFT_4_PENALTY=0.761472
1909
PRIMER_RIGHT_4_PENALTY=0.396944
1910
PRIMER_LEFT_4_SEQUENCE=TAGAGTTCTCAGATGCCCCC
1911
PRIMER_RIGHT_4_SEQUENCE=CATGGCTTTGGTGAGCTTCT
1912
PRIMER_LEFT_4=162,20
1913
PRIMER_RIGHT_4=328,20
1914
PRIMER_LEFT_4_TM=59.239
1915
PRIMER_RIGHT_4_TM=60.397
1916
PRIMER_LEFT_4_GC_PERCENT=55.000
1917
PRIMER_RIGHT_4_GC_PERCENT=50.000
1918
PRIMER_LEFT_4_SELF_ANY=5.00
1919
PRIMER_RIGHT_4_SELF_ANY=4.00
1920
PRIMER_LEFT_4_SELF_END=0.00
1921
PRIMER_RIGHT_4_SELF_END=1.00
1922
PRIMER_LEFT_4_END_STABILITY=12.4000
1923
PRIMER_RIGHT_4_END_STABILITY=6.7000
1924
PRIMER_PAIR_4_COMPL_ANY=4.00
1925
PRIMER_PAIR_4_COMPL_END=4.00
1926
PRIMER_PRODUCT_SIZE_4=167
1927
=
1928
MARKER_NAME=21.RHAP33FF9.seq
1929
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNTATGAAGTAGCAGTGAAACTAAGTTTATGGCTGGGAGTCATTAGAACATGAGGAACCGGTTAAAGGGTGACAGCGTTAGGACTGAGAACCTGTGCTTTAGAGCAAGCCTCAACTGCCCACTTGCAGACAGCAGTTCTCCAGTCATTTTCAACTTGTTCATTTCCAATGGAGACAAGATATCTATTTATCACGTGCAGCATGATAAATAANNNNNNNNNNNNNNNNNACATCCCCAACAGTGAGGACCAGGCTCTACTTGAAAACCCATTTCCACACCCACTCTCACTCATGAACAGACATACACATAATGAATGTATTTTTGTGAAGCACCCATTTCATACCTACCAAGAAAAATAATTCATTTTCT
1930
INCLUDED_REGION=27,367
1931
TARGET=236,17,AT
1932
PRIMER_COMMENT=CL is CCTGTGCTTTAGAGCAAGCC
1933
PRIMER_COMMENT=CR is GTCCTCACTGTTGGGGATGT
1934
PRIMER_PAIR_PENALTY=0.3373
1935
PRIMER_LEFT_PENALTY=0.154023
1936
PRIMER_RIGHT_PENALTY=0.183238
1937
PRIMER_LEFT_SEQUENCE=CCTGTGCTTTAGAGCAAGCC
1938
PRIMER_RIGHT_SEQUENCE=GTCCTCACTGTTGGGGATGT
1939
PRIMER_LEFT=115,20
1940
PRIMER_RIGHT=272,20
1941
PRIMER_LEFT_TM=60.154
1942
PRIMER_RIGHT_TM=59.817
1943
PRIMER_LEFT_GC_PERCENT=55.000
1944
PRIMER_RIGHT_GC_PERCENT=55.000
1945
PRIMER_LEFT_SELF_ANY=8.00
1946
PRIMER_RIGHT_SELF_ANY=5.00
1947
PRIMER_LEFT_SELF_END=4.00
1948
PRIMER_RIGHT_SELF_END=0.00
1949
PRIMER_LEFT_END_STABILITY=9.7000
1950
PRIMER_RIGHT_END_STABILITY=6.3000
1951
PRIMER_PAIR_COMPL_ANY=3.00
1952
PRIMER_PAIR_COMPL_END=1.00
1953
PRIMER_PRODUCT_SIZE=158
1954
PRIMER_PAIR_PENALTY_1=0.5166
1955
PRIMER_LEFT_1_PENALTY=0.333322
1956
PRIMER_RIGHT_1_PENALTY=0.183238
1957
PRIMER_LEFT_1_SEQUENCE=TGCAGACAGCAGTTCTCCAG
1958
PRIMER_RIGHT_1_SEQUENCE=GTCCTCACTGTTGGGGATGT
1959
PRIMER_LEFT_1=148,20
1960
PRIMER_RIGHT_1=272,20
1961
PRIMER_LEFT_1_TM=60.333
1962
PRIMER_RIGHT_1_TM=59.817
1963
PRIMER_LEFT_1_GC_PERCENT=55.000
1964
PRIMER_RIGHT_1_GC_PERCENT=55.000
1965
PRIMER_LEFT_1_SELF_ANY=6.00
1966
PRIMER_RIGHT_1_SELF_ANY=5.00
1967
PRIMER_LEFT_1_SELF_END=2.00
1968
PRIMER_RIGHT_1_SELF_END=0.00
1969
PRIMER_LEFT_1_END_STABILITY=8.2000
1970
PRIMER_RIGHT_1_END_STABILITY=6.3000
1971
PRIMER_PAIR_1_COMPL_ANY=5.00
1972
PRIMER_PAIR_1_COMPL_END=2.00
1973
PRIMER_PRODUCT_SIZE_1=125
1974
PRIMER_PAIR_PENALTY_2=0.5611
1975
PRIMER_LEFT_2_PENALTY=0.377815
1976
PRIMER_RIGHT_2_PENALTY=0.183238
1977
PRIMER_LEFT_2_SEQUENCE=CCACTTGCAGACAGCAGTTC
1978
PRIMER_RIGHT_2_SEQUENCE=GTCCTCACTGTTGGGGATGT
1979
PRIMER_LEFT_2=143,20
1980
PRIMER_RIGHT_2=272,20
1981
PRIMER_LEFT_2_TM=59.622
1982
PRIMER_RIGHT_2_TM=59.817
1983
PRIMER_LEFT_2_GC_PERCENT=55.000
1984
PRIMER_RIGHT_2_GC_PERCENT=55.000
1985
PRIMER_LEFT_2_SELF_ANY=5.00
1986
PRIMER_RIGHT_2_SELF_ANY=5.00
1987
PRIMER_LEFT_2_SELF_END=2.00
1988
PRIMER_RIGHT_2_SELF_END=0.00
1989
PRIMER_LEFT_2_END_STABILITY=6.4000
1990
PRIMER_RIGHT_2_END_STABILITY=6.3000
1991
PRIMER_PAIR_2_COMPL_ANY=5.00
1992
PRIMER_PAIR_2_COMPL_END=1.00
1993
PRIMER_PRODUCT_SIZE_2=130
1994
PRIMER_PAIR_PENALTY_3=0.6266
1995
PRIMER_LEFT_3_PENALTY=0.333322
1996
PRIMER_RIGHT_3_PENALTY=0.293316
1997
PRIMER_LEFT_3_SEQUENCE=TGCAGACAGCAGTTCTCCAG
1998
PRIMER_RIGHT_3_SEQUENCE=TGAGTGAGAGTGGGTGTGGA
1999
PRIMER_LEFT_3=148,20
2000
PRIMER_RIGHT_3=315,20
2001
PRIMER_LEFT_3_TM=60.333
2002
PRIMER_RIGHT_3_TM=60.293
2003
PRIMER_LEFT_3_GC_PERCENT=55.000
2004
PRIMER_RIGHT_3_GC_PERCENT=55.000
2005
PRIMER_LEFT_3_SELF_ANY=6.00
2006
PRIMER_RIGHT_3_SELF_ANY=1.00
2007
PRIMER_LEFT_3_SELF_END=2.00
2008
PRIMER_RIGHT_3_SELF_END=0.00
2009
PRIMER_LEFT_3_END_STABILITY=8.2000
2010
PRIMER_RIGHT_3_END_STABILITY=7.9000
2011
PRIMER_PAIR_3_COMPL_ANY=4.00
2012
PRIMER_PAIR_3_COMPL_END=3.00
2013
PRIMER_PRODUCT_SIZE_3=168
2014
PRIMER_PAIR_PENALTY_4=0.6711
2015
PRIMER_LEFT_4_PENALTY=0.377815
2016
PRIMER_RIGHT_4_PENALTY=0.293316
2017
PRIMER_LEFT_4_SEQUENCE=CCACTTGCAGACAGCAGTTC
2018
PRIMER_RIGHT_4_SEQUENCE=TGAGTGAGAGTGGGTGTGGA
2019
PRIMER_LEFT_4=143,20
2020
PRIMER_RIGHT_4=315,20
2021
PRIMER_LEFT_4_TM=59.622
2022
PRIMER_RIGHT_4_TM=60.293
2023
PRIMER_LEFT_4_GC_PERCENT=55.000
2024
PRIMER_RIGHT_4_GC_PERCENT=55.000
2025
PRIMER_LEFT_4_SELF_ANY=5.00
2026
PRIMER_RIGHT_4_SELF_ANY=1.00
2027
PRIMER_LEFT_4_SELF_END=2.00
2028
PRIMER_RIGHT_4_SELF_END=0.00
2029
PRIMER_LEFT_4_END_STABILITY=6.4000
2030
PRIMER_RIGHT_4_END_STABILITY=7.9000
2031
PRIMER_PAIR_4_COMPL_ANY=5.00
2032
PRIMER_PAIR_4_COMPL_END=2.00
2033
PRIMER_PRODUCT_SIZE_4=173
2034
=
2035
MARKER_NAME=21.RHAP34FB9.seq
2036
SEQUENCE=NNNNNNNNNNNNNNNNNNNNGTAAAACATTCCTTCTTTCTAGCAATCATTCGTATACAAACTAAAACATAATCTGTGTCTTGCTTACAAGCATTTCAAATATTCAAAAATGTTATTTNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTAGTGTATGTCTATGTTTATATGTGTCCTTGTTTATATGTGTGTTCATGTTTATAGGTATGTGTCTATGTTTATATGTGTGTCCATGTTTATATGTGTGCCTGTTCATATGTGTGTTCATGNTTATAGGTGTGTGTCCATGTTTATAT
2037
INCLUDED_REGION=20,274
2038
TARGET=117,26,TG
2039
PRIMER_COMMENT=CL is TGTCTTGCTTACAAGCATTTCAA
2040
PRIMER_COMMENT=CR is CAGGCACACATATAAACATGGA
2041
PRIMER_PAIR_PENALTY=6.6221
2042
PRIMER_LEFT_PENALTY=3.061184
2043
PRIMER_RIGHT_PENALTY=3.560938
2044
PRIMER_LEFT_SEQUENCE=TGTCTTGCTTACAAGCATTTCAA
2045
PRIMER_RIGHT_SEQUENCE=CAGGCACACATATAAACATGGA
2046
PRIMER_LEFT=75,23
2047
PRIMER_RIGHT=248,22
2048
PRIMER_LEFT_TM=59.939
2049
PRIMER_RIGHT_TM=58.439
2050
PRIMER_LEFT_GC_PERCENT=34.783
2051
PRIMER_RIGHT_GC_PERCENT=40.909
2052
PRIMER_LEFT_SELF_ANY=8.00
2053
PRIMER_RIGHT_SELF_ANY=4.00
2054
PRIMER_LEFT_SELF_END=4.00
2055
PRIMER_RIGHT_SELF_END=0.00
2056
PRIMER_LEFT_END_STABILITY=7.3000
2057
PRIMER_RIGHT_END_STABILITY=8.1000
2058
PRIMER_PAIR_COMPL_ANY=4.00
2059
PRIMER_PAIR_COMPL_END=2.00
2060
PRIMER_PRODUCT_SIZE=174
2061
PRIMER_PAIR_PENALTY_1=6.7671
2062
PRIMER_LEFT_1_PENALTY=3.061184
2063
PRIMER_RIGHT_1_PENALTY=3.705897
2064
PRIMER_LEFT_1_SEQUENCE=TGTCTTGCTTACAAGCATTTCAA
2065
PRIMER_RIGHT_1_SEQUENCE=GGCACACATATAAACATGGACA
2066
PRIMER_LEFT_1=75,23
2067
PRIMER_RIGHT_1=246,22
2068
PRIMER_LEFT_1_TM=59.939
2069
PRIMER_RIGHT_1_TM=58.294
2070
PRIMER_LEFT_1_GC_PERCENT=34.783
2071
PRIMER_RIGHT_1_GC_PERCENT=40.909
2072
PRIMER_LEFT_1_SELF_ANY=8.00
2073
PRIMER_RIGHT_1_SELF_ANY=4.00
2074
PRIMER_LEFT_1_SELF_END=4.00
2075
PRIMER_RIGHT_1_SELF_END=2.00
2076
PRIMER_LEFT_1_END_STABILITY=7.3000
2077
PRIMER_RIGHT_1_END_STABILITY=7.9000
2078
PRIMER_PAIR_1_COMPL_ANY=5.00
2079
PRIMER_PAIR_1_COMPL_END=3.00
2080
PRIMER_PRODUCT_SIZE_1=172
2081
PRIMER_PAIR_PENALTY_2=6.7769
2082
PRIMER_LEFT_2_PENALTY=3.061184
2083
PRIMER_RIGHT_2_PENALTY=3.715687
2084
PRIMER_LEFT_2_SEQUENCE=TGTCTTGCTTACAAGCATTTCAA
2085
PRIMER_RIGHT_2_SEQUENCE=CAGGCACACATATAAACATGGAC
2086
PRIMER_LEFT_2=75,23
2087
PRIMER_RIGHT_2=248,23
2088
PRIMER_LEFT_2_TM=59.939
2089
PRIMER_RIGHT_2_TM=59.284
2090
PRIMER_LEFT_2_GC_PERCENT=34.783
2091
PRIMER_RIGHT_2_GC_PERCENT=43.478
2092
PRIMER_LEFT_2_SELF_ANY=8.00
2093
PRIMER_RIGHT_2_SELF_ANY=4.00
2094
PRIMER_LEFT_2_SELF_END=4.00
2095
PRIMER_RIGHT_2_SELF_END=1.00
2096
PRIMER_LEFT_2_END_STABILITY=7.3000
2097
PRIMER_RIGHT_2_END_STABILITY=7.9000
2098
PRIMER_PAIR_2_COMPL_ANY=4.00
2099
PRIMER_PAIR_2_COMPL_END=1.00
2100
PRIMER_PRODUCT_SIZE_2=174
2101
PRIMER_PAIR_PENALTY_3=6.7769
2102
PRIMER_LEFT_3_PENALTY=3.061184
2103
PRIMER_RIGHT_3_PENALTY=3.715687
2104
PRIMER_LEFT_3_SEQUENCE=TGTCTTGCTTACAAGCATTTCAA
2105
PRIMER_RIGHT_3_SEQUENCE=ACAGGCACACATATAAACATGGA
2106
PRIMER_LEFT_3=75,23
2107
PRIMER_RIGHT_3=249,23
2108
PRIMER_LEFT_3_TM=59.939
2109
PRIMER_RIGHT_3_TM=59.284
2110
PRIMER_LEFT_3_GC_PERCENT=34.783
2111
PRIMER_RIGHT_3_GC_PERCENT=39.130
2112
PRIMER_LEFT_3_SELF_ANY=8.00
2113
PRIMER_RIGHT_3_SELF_ANY=4.00
2114
PRIMER_LEFT_3_SELF_END=4.00
2115
PRIMER_RIGHT_3_SELF_END=0.00
2116
PRIMER_LEFT_3_END_STABILITY=7.3000
2117
PRIMER_RIGHT_3_END_STABILITY=8.1000
2118
PRIMER_PAIR_3_COMPL_ANY=4.00
2119
PRIMER_PAIR_3_COMPL_END=2.00
2120
PRIMER_PRODUCT_SIZE_3=175
2121
PRIMER_PAIR_PENALTY_4=6.7769
2122
PRIMER_LEFT_4_PENALTY=3.061184
2123
PRIMER_RIGHT_4_PENALTY=3.715687
2124
PRIMER_LEFT_4_SEQUENCE=TGTCTTGCTTACAAGCATTTCAA
2125
PRIMER_RIGHT_4_SEQUENCE=AGGCACACATATAAACATGGACA
2126
PRIMER_LEFT_4=75,23
2127
PRIMER_RIGHT_4=247,23
2128
PRIMER_LEFT_4_TM=59.939
2129
PRIMER_RIGHT_4_TM=59.284
2130
PRIMER_LEFT_4_GC_PERCENT=34.783
2131
PRIMER_RIGHT_4_GC_PERCENT=39.130
2132
PRIMER_LEFT_4_SELF_ANY=8.00
2133
PRIMER_RIGHT_4_SELF_ANY=4.00
2134
PRIMER_LEFT_4_SELF_END=4.00
2135
PRIMER_RIGHT_4_SELF_END=2.00
2136
PRIMER_LEFT_4_END_STABILITY=7.3000
2137
PRIMER_RIGHT_4_END_STABILITY=7.9000
2138
PRIMER_PAIR_4_COMPL_ANY=5.00
2139
PRIMER_PAIR_4_COMPL_END=2.00
2140
PRIMER_PRODUCT_SIZE_4=173
2141
=
2142
MARKER_NAME=22.RHAP33FF10.seq
2143
SEQUENCE=NNNNNNNNNNNNNNNNNNCTGCCTCCCAGGCAGGATGGCCGCTCACGTTGAGGGATGGCTCCCTACAGAGGCAGCAGGGGTAATGGGGAGCTTATCTTACTTCATTCACTCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTGANNNNNNNNNNNNNNNNCTTATAATTTTGGGAGTTTTTTTCTTTTCATTATCTTTGGCCTCCAAAGATTTTAAAGAGTCNAGCCCAACTTCTTGCAGGTCTTCAGGGAACGCCCCAGTAAATCAAGCTGCAGGCAATGGACANGGAGAATACACCTTCCTCAAGCNCCCGAAAGAAGAGCCAAAAGGNAGAAGCCCACTGANNCCCGC
2144
INCLUDED_REGION=18,370
2145
TARGET=113,63,TG
2146
TARGET=181,16,TG
2147
PRIMER_COMMENT=CL is GGGGTAATGGGGAGCTTATC
2148
PRIMER_COMMENT=CR is TTGGAGGCCAAAGATAATGAA
2149
PRIMER_PAIR_PENALTY=1.8393
2150
PRIMER_LEFT_PENALTY=0.372603
2151
PRIMER_RIGHT_PENALTY=1.466705
2152
PRIMER_LEFT_SEQUENCE=GGGGTAATGGGGAGCTTATC
2153
PRIMER_RIGHT_SEQUENCE=TGGAGGCCAAAGATAATGAAA
2154
PRIMER_LEFT=76,20
2155
PRIMER_RIGHT=242,21
2156
PRIMER_LEFT_TM=59.627
2157
PRIMER_RIGHT_TM=59.533
2158
PRIMER_LEFT_GC_PERCENT=55.000
2159
PRIMER_RIGHT_GC_PERCENT=38.095
2160
PRIMER_LEFT_SELF_ANY=4.00
2161
PRIMER_RIGHT_SELF_ANY=4.00
2162
PRIMER_LEFT_SELF_END=1.00
2163
PRIMER_RIGHT_SELF_END=0.00
2164
PRIMER_LEFT_END_STABILITY=5.9000
2165
PRIMER_RIGHT_END_STABILITY=7.3000
2166
PRIMER_PAIR_COMPL_ANY=5.00
2167
PRIMER_PAIR_COMPL_END=2.00
2168
PRIMER_PRODUCT_SIZE=167
2169
PRIMER_PAIR_PENALTY_1=1.8393
2170
PRIMER_LEFT_1_PENALTY=0.372603
2171
PRIMER_RIGHT_1_PENALTY=1.466705
2172
PRIMER_LEFT_1_SEQUENCE=GGGGTAATGGGGAGCTTATC
2173
PRIMER_RIGHT_1_SEQUENCE=TTGGAGGCCAAAGATAATGAA
2174
PRIMER_LEFT_1=76,20
2175
PRIMER_RIGHT_1=243,21
2176
PRIMER_LEFT_1_TM=59.627
2177
PRIMER_RIGHT_1_TM=59.533
2178
PRIMER_LEFT_1_GC_PERCENT=55.000
2179
PRIMER_RIGHT_1_GC_PERCENT=38.095
2180
PRIMER_LEFT_1_SELF_ANY=4.00
2181
PRIMER_RIGHT_1_SELF_ANY=5.00
2182
PRIMER_LEFT_1_SELF_END=1.00
2183
PRIMER_RIGHT_1_SELF_END=0.00
2184
PRIMER_LEFT_1_END_STABILITY=5.9000
2185
PRIMER_RIGHT_1_END_STABILITY=6.9000
2186
PRIMER_PAIR_1_COMPL_ANY=5.00
2187
PRIMER_PAIR_1_COMPL_END=2.00
2188
PRIMER_PRODUCT_SIZE_1=168
2189
PRIMER_PAIR_PENALTY_2=1.8393
2190
PRIMER_LEFT_2_PENALTY=0.372603
2191
PRIMER_RIGHT_2_PENALTY=1.466705
2192
PRIMER_LEFT_2_SEQUENCE=GGGGTAATGGGGAGCTTATC
2193
PRIMER_RIGHT_2_SEQUENCE=TTTGGAGGCCAAAGATAATGA
2194
PRIMER_LEFT_2=76,20
2195
PRIMER_RIGHT_2=244,21
2196
PRIMER_LEFT_2_TM=59.627
2197
PRIMER_RIGHT_2_TM=59.533
2198
PRIMER_LEFT_2_GC_PERCENT=55.000
2199
PRIMER_RIGHT_2_GC_PERCENT=38.095
2200
PRIMER_LEFT_2_SELF_ANY=4.00
2201
PRIMER_RIGHT_2_SELF_ANY=7.00
2202
PRIMER_LEFT_2_SELF_END=1.00
2203
PRIMER_RIGHT_2_SELF_END=2.00
2204
PRIMER_LEFT_2_END_STABILITY=5.9000
2205
PRIMER_RIGHT_2_END_STABILITY=6.9000
2206
PRIMER_PAIR_2_COMPL_ANY=5.00
2207
PRIMER_PAIR_2_COMPL_END=3.00
2208
PRIMER_PRODUCT_SIZE_2=169
2209
PRIMER_PAIR_PENALTY_3=2.2125
2210
PRIMER_LEFT_3_PENALTY=0.372603
2211
PRIMER_RIGHT_3_PENALTY=1.839872
2212
PRIMER_LEFT_3_SEQUENCE=GGGGTAATGGGGAGCTTATC
2213
PRIMER_RIGHT_3_SEQUENCE=TGGAGGCCAAAGATAATGAA
2214
PRIMER_LEFT_3=76,20
2215
PRIMER_RIGHT_3=242,20
2216
PRIMER_LEFT_3_TM=59.627
2217
PRIMER_RIGHT_3_TM=58.160
2218
PRIMER_LEFT_3_GC_PERCENT=55.000
2219
PRIMER_RIGHT_3_GC_PERCENT=40.000
2220
PRIMER_LEFT_3_SELF_ANY=4.00
2221
PRIMER_RIGHT_3_SELF_ANY=4.00
2222
PRIMER_LEFT_3_SELF_END=1.00
2223
PRIMER_RIGHT_3_SELF_END=0.00
2224
PRIMER_LEFT_3_END_STABILITY=5.9000
2225
PRIMER_RIGHT_3_END_STABILITY=6.9000
2226
PRIMER_PAIR_3_COMPL_ANY=5.00
2227
PRIMER_PAIR_3_COMPL_END=2.00
2228
PRIMER_PRODUCT_SIZE_3=167
2229
PRIMER_PAIR_PENALTY_4=2.2125
2230
PRIMER_LEFT_4_PENALTY=0.372603
2231
PRIMER_RIGHT_4_PENALTY=1.839872
2232
PRIMER_LEFT_4_SEQUENCE=GGGGTAATGGGGAGCTTATC
2233
PRIMER_RIGHT_4_SEQUENCE=TTGGAGGCCAAAGATAATGA
2234
PRIMER_LEFT_4=76,20
2235
PRIMER_RIGHT_4=243,20
2236
PRIMER_LEFT_4_TM=59.627
2237
PRIMER_RIGHT_4_TM=58.160
2238
PRIMER_LEFT_4_GC_PERCENT=55.000
2239
PRIMER_RIGHT_4_GC_PERCENT=40.000
2240
PRIMER_LEFT_4_SELF_ANY=4.00
2241
PRIMER_RIGHT_4_SELF_ANY=5.00
2242
PRIMER_LEFT_4_SELF_END=1.00
2243
PRIMER_RIGHT_4_SELF_END=2.00
2244
PRIMER_LEFT_4_END_STABILITY=5.9000
2245
PRIMER_RIGHT_4_END_STABILITY=6.9000
2246
PRIMER_PAIR_4_COMPL_ANY=5.00
2247
PRIMER_PAIR_4_COMPL_END=3.00
2248
PRIMER_PRODUCT_SIZE_4=168
2249
=
2250
MARKER_NAME=22.RHAP34FB10.seq
2251
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTACACNCNCCCATGGTGAATGTGTTTCGNGCTCTTTTTCNTGGCTAATCAAGNGTTACCCCNTGGGGACATGAATCATGGAGAAACACAATACNAGAGAAANNTCCCAAGTTACCANNAGACCCAGTCCANGAATAGANGAGTAANNNNNNNNNNNNNNNNNAAAATATTGAAAANCCACTCACATACACAGNCATCNTACNTAACAAGNCACATACACATNNCACNGAGACACCACATGACACATACNCATAAATNAACAGACATAGAGAGAGACATAAAGACATGGACNCAAAGAGAANTATCATACACACAANGAAAGTAAAAAAATTACCACATTCAAACNCACNCANNGAGGACAANCAATTGNCAATTAAGAAAAGNCATACAAAAAATNCAAATNGAGANNGAT
2252
INCLUDED_REGION=23,438
2253
TARGET=23,27,NC
2254
TARGET=195,17,AG
2255
PRIMER_COMMENT=CL is
2256
PRIMER_COMMENT=CR is
2257
=
2258
MARKER_NAME=22.RHAP34FF10.seq
2259
SEQUENCE=NNNNNNNNNCCTCATATCCGGCATACATGCCTTCCCTGCCATCCTCAGCTGTATCCCCACAAACCTGAACTAAAATAAACCCTTCCCTGTTGCTTTTCTTAGACATTCTGTCACAGCAGTGAGAAAGGTAACTAAAGCAGAATCCTGGTTCCAGAAGTGGGTTGTCCTTGTGATAAACCCATCTCATGGCGCCTAGGCTTTTGCAATTGGTTTATGTGAAGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTTGTGATGGTGGGATGNT
2260
INCLUDED_REGION=9,284
2261
TARGET=223,50,GT
2262
PRIMER_COMMENT=CL is GCAGAATCCTGGTTCCAGAA
2263
PRIMER_COMMENT=CR is CATCCCACCATCACAACC
2264
PRIMER_PAIR_PENALTY=4.2169
2265
PRIMER_LEFT_PENALTY=0.195358
2266
PRIMER_RIGHT_PENALTY=4.021553
2267
PRIMER_LEFT_SEQUENCE=GCAGAATCCTGGTTCCAGAA
2268
PRIMER_RIGHT_SEQUENCE=CATCCCACCATCACAACC
2269
PRIMER_LEFT=136,20
2270
PRIMER_RIGHT=290,18
2271
PRIMER_LEFT_TM=60.195
2272
PRIMER_RIGHT_TM=57.978
2273
PRIMER_LEFT_GC_PERCENT=50.000
2274
PRIMER_RIGHT_GC_PERCENT=55.556
2275
PRIMER_LEFT_SELF_ANY=6.00
2276
PRIMER_RIGHT_SELF_ANY=2.00
2277
PRIMER_LEFT_SELF_END=6.00
2278
PRIMER_RIGHT_SELF_END=0.00
2279
PRIMER_LEFT_END_STABILITY=7.0000
2280
PRIMER_RIGHT_END_STABILITY=8.2000
2281
PRIMER_PAIR_COMPL_ANY=4.00
2282
PRIMER_PAIR_COMPL_END=1.00
2283
PRIMER_PRODUCT_SIZE=155
2284
PRIMER_PAIR_PENALTY_1=4.2185
2285
PRIMER_LEFT_1_PENALTY=0.196903
2286
PRIMER_RIGHT_1_PENALTY=4.021553
2287
PRIMER_LEFT_1_SEQUENCE=AGCAGAATCCTGGTTCCAGA
2288
PRIMER_RIGHT_1_SEQUENCE=CATCCCACCATCACAACC
2289
PRIMER_LEFT_1=135,20
2290
PRIMER_RIGHT_1=290,18
2291
PRIMER_LEFT_1_TM=59.803
2292
PRIMER_RIGHT_1_TM=57.978
2293
PRIMER_LEFT_1_GC_PERCENT=50.000
2294
PRIMER_RIGHT_1_GC_PERCENT=55.556
2295
PRIMER_LEFT_1_SELF_ANY=6.00
2296
PRIMER_RIGHT_1_SELF_ANY=2.00
2297
PRIMER_LEFT_1_SELF_END=4.00
2298
PRIMER_RIGHT_1_SELF_END=0.00
2299
PRIMER_LEFT_1_END_STABILITY=8.2000
2300
PRIMER_RIGHT_1_END_STABILITY=8.2000
2301
PRIMER_PAIR_1_COMPL_ANY=4.00
2302
PRIMER_PAIR_1_COMPL_END=2.00
2303
PRIMER_PRODUCT_SIZE_1=156
2304
PRIMER_PAIR_PENALTY_2=4.3511
2305
PRIMER_LEFT_2_PENALTY=0.329550
2306
PRIMER_RIGHT_2_PENALTY=4.021553
2307
PRIMER_LEFT_2_SEQUENCE=AAAGCAGAATCCTGGTTCCA
2308
PRIMER_RIGHT_2_SEQUENCE=CATCCCACCATCACAACC
2309
PRIMER_LEFT_2=133,20
2310
PRIMER_RIGHT_2=290,18
2311
PRIMER_LEFT_2_TM=59.670
2312
PRIMER_RIGHT_2_TM=57.978
2313
PRIMER_LEFT_2_GC_PERCENT=45.000
2314
PRIMER_RIGHT_2_GC_PERCENT=55.556
2315
PRIMER_LEFT_2_SELF_ANY=8.00
2316
PRIMER_RIGHT_2_SELF_ANY=2.00
2317
PRIMER_LEFT_2_SELF_END=4.00
2318
PRIMER_RIGHT_2_SELF_END=0.00
2319
PRIMER_LEFT_2_END_STABILITY=8.5000
2320
PRIMER_RIGHT_2_END_STABILITY=8.2000
2321
PRIMER_PAIR_2_COMPL_ANY=4.00
2322
PRIMER_PAIR_2_COMPL_END=1.00
2323
PRIMER_PRODUCT_SIZE_2=158
2324
PRIMER_PAIR_PENALTY_3=4.4209
2325
PRIMER_LEFT_3_PENALTY=0.399325
2326
PRIMER_RIGHT_3_PENALTY=4.021553
2327
PRIMER_LEFT_3_SEQUENCE=TGGTTCCAGAAGTGGGTTGT
2328
PRIMER_RIGHT_3_SEQUENCE=CATCCCACCATCACAACC
2329
PRIMER_LEFT_3=145,20
2330
PRIMER_RIGHT_3=290,18
2331
PRIMER_LEFT_3_TM=60.399
2332
PRIMER_RIGHT_3_TM=57.978
2333
PRIMER_LEFT_3_GC_PERCENT=50.000
2334
PRIMER_RIGHT_3_GC_PERCENT=55.556
2335
PRIMER_LEFT_3_SELF_ANY=4.00
2336
PRIMER_RIGHT_3_SELF_ANY=2.00
2337
PRIMER_LEFT_3_SELF_END=0.00
2338
PRIMER_RIGHT_3_SELF_END=0.00
2339
PRIMER_LEFT_3_END_STABILITY=6.4000
2340
PRIMER_RIGHT_3_END_STABILITY=8.2000
2341
PRIMER_PAIR_3_COMPL_ANY=6.00
2342
PRIMER_PAIR_3_COMPL_END=6.00
2343
PRIMER_PRODUCT_SIZE_3=146
2344
PRIMER_PAIR_PENALTY_4=4.4724
2345
PRIMER_LEFT_4_PENALTY=0.450843
2346
PRIMER_RIGHT_4_PENALTY=4.021553
2347
PRIMER_LEFT_4_SEQUENCE=TCCAGAAGTGGGTTGTCCTT
2348
PRIMER_RIGHT_4_SEQUENCE=CATCCCACCATCACAACC
2349
PRIMER_LEFT_4=149,20
2350
PRIMER_RIGHT_4=290,18
2351
PRIMER_LEFT_4_TM=59.549
2352
PRIMER_RIGHT_4_TM=57.978
2353
PRIMER_LEFT_4_GC_PERCENT=50.000
2354
PRIMER_RIGHT_4_GC_PERCENT=55.556
2355
PRIMER_LEFT_4_SELF_ANY=3.00
2356
PRIMER_RIGHT_4_SELF_ANY=2.00
2357
PRIMER_LEFT_4_SELF_END=0.00
2358
PRIMER_RIGHT_4_SELF_END=0.00
2359
PRIMER_LEFT_4_END_STABILITY=8.2000
2360
PRIMER_RIGHT_4_END_STABILITY=8.2000
2361
PRIMER_PAIR_4_COMPL_ANY=6.00
2362
PRIMER_PAIR_4_COMPL_END=4.00
2363
PRIMER_PRODUCT_SIZE_4=142
2364
=
2365
MARKER_NAME=23.RHAP34FF11.seq
2366
SEQUENCE=NNNNNNNNNNNNNNNNNNNCCTACCAATTGTTCTTTATTTACAGGGAGGTCTACTCTGCCTCCTCATCCAGGCTTTCTTTCATTCATCTCCTTAGCTGATTGCATTAGATGCACACATTCATACACACAGGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTGTGCTTCCCTGCAGAGGTCAAGTCTGGGAATAATACAAAGACTTCTCTGTAGGTCCACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
2367
INCLUDED_REGION=19,240
2368
TARGET=133,64,CA
2369
PRIMER_COMMENT=CL is CCTCCTCATCCAGGCTTTCT
2370
PRIMER_COMMENT=CR is AGACTTGACCTCTGCAGGGA
2371
PRIMER_PAIR_PENALTY=0.7394
2372
PRIMER_LEFT_PENALTY=0.725022
2373
PRIMER_RIGHT_PENALTY=0.014419
2374
PRIMER_LEFT_SEQUENCE=CCTCCTCATCCAGGCTTTCT
2375
PRIMER_RIGHT_SEQUENCE=AGACTTGACCTCTGCAGGGA
2376
PRIMER_LEFT=58,20
2377
PRIMER_RIGHT=224,20
2378
PRIMER_LEFT_TM=60.725
2379
PRIMER_RIGHT_TM=59.986
2380
PRIMER_LEFT_GC_PERCENT=55.000
2381
PRIMER_RIGHT_GC_PERCENT=55.000
2382
PRIMER_LEFT_SELF_ANY=3.00
2383
PRIMER_RIGHT_SELF_ANY=6.00
2384
PRIMER_LEFT_SELF_END=0.00
2385
PRIMER_RIGHT_SELF_END=4.00
2386
PRIMER_LEFT_END_STABILITY=7.0000
2387
PRIMER_RIGHT_END_STABILITY=9.4000
2388
PRIMER_PAIR_COMPL_ANY=4.00
2389
PRIMER_PAIR_COMPL_END=1.00
2390
PRIMER_PRODUCT_SIZE=167
2391
PRIMER_PAIR_PENALTY_1=1.1108
2392
PRIMER_LEFT_1_PENALTY=0.725022
2393
PRIMER_RIGHT_1_PENALTY=0.385756
2394
PRIMER_LEFT_1_SEQUENCE=CCTCCTCATCCAGGCTTTCT
2395
PRIMER_RIGHT_1_SEQUENCE=TTCCCAGACTTGACCTCTGC
2396
PRIMER_LEFT_1=58,20
2397
PRIMER_RIGHT_1=229,20
2398
PRIMER_LEFT_1_TM=60.725
2399
PRIMER_RIGHT_1_TM=60.386
2400
PRIMER_LEFT_1_GC_PERCENT=55.000
2401
PRIMER_RIGHT_1_GC_PERCENT=55.000
2402
PRIMER_LEFT_1_SELF_ANY=3.00
2403
PRIMER_RIGHT_1_SELF_ANY=5.00
2404
PRIMER_LEFT_1_SELF_END=0.00
2405
PRIMER_RIGHT_1_SELF_END=3.00
2406
PRIMER_LEFT_1_END_STABILITY=7.0000
2407
PRIMER_RIGHT_1_END_STABILITY=8.2000
2408
PRIMER_PAIR_1_COMPL_ANY=4.00
2409
PRIMER_PAIR_1_COMPL_END=0.00
2410
PRIMER_PRODUCT_SIZE_1=172
2411
PRIMER_PAIR_PENALTY_2=1.1108
2412
PRIMER_LEFT_2_PENALTY=0.725022
2413
PRIMER_RIGHT_2_PENALTY=0.385756
2414
PRIMER_LEFT_2_SEQUENCE=CCTCCTCATCCAGGCTTTCT
2415
PRIMER_RIGHT_2_SEQUENCE=GACTTGACCTCTGCAGGGAA
2416
PRIMER_LEFT_2=58,20
2417
PRIMER_RIGHT_2=223,20
2418
PRIMER_LEFT_2_TM=60.725
2419
PRIMER_RIGHT_2_TM=60.386
2420
PRIMER_LEFT_2_GC_PERCENT=55.000
2421
PRIMER_RIGHT_2_GC_PERCENT=55.000
2422
PRIMER_LEFT_2_SELF_ANY=3.00
2423
PRIMER_RIGHT_2_SELF_ANY=6.00
2424
PRIMER_LEFT_2_SELF_END=0.00
2425
PRIMER_RIGHT_2_SELF_END=2.00
2426
PRIMER_LEFT_2_END_STABILITY=7.0000
2427
PRIMER_RIGHT_2_END_STABILITY=9.7000
2428
PRIMER_PAIR_2_COMPL_ANY=3.00
2429
PRIMER_PAIR_2_COMPL_END=2.00
2430
PRIMER_PRODUCT_SIZE_2=166
2431
PRIMER_PAIR_PENALTY_3=1.1532
2432
PRIMER_LEFT_3_PENALTY=1.138774
2433
PRIMER_RIGHT_3_PENALTY=0.014419
2434
PRIMER_LEFT_3_SEQUENCE=TCCTCATCCAGGCTTTCTTT
2435
PRIMER_RIGHT_3_SEQUENCE=AGACTTGACCTCTGCAGGGA
2436
PRIMER_LEFT_3=60,20
2437
PRIMER_RIGHT_3=224,20
2438
PRIMER_LEFT_3_TM=58.861
2439
PRIMER_RIGHT_3_TM=59.986
2440
PRIMER_LEFT_3_GC_PERCENT=45.000
2441
PRIMER_RIGHT_3_GC_PERCENT=55.000
2442
PRIMER_LEFT_3_SELF_ANY=3.00
2443
PRIMER_RIGHT_3_SELF_ANY=6.00
2444
PRIMER_LEFT_3_SELF_END=0.00
2445
PRIMER_RIGHT_3_SELF_END=4.00
2446
PRIMER_LEFT_3_END_STABILITY=7.0000
2447
PRIMER_RIGHT_3_END_STABILITY=9.4000
2448
PRIMER_PAIR_3_COMPL_ANY=4.00
2449
PRIMER_PAIR_3_COMPL_END=1.00
2450
PRIMER_PRODUCT_SIZE_3=165
2451
PRIMER_PAIR_PENALTY_4=1.1532
2452
PRIMER_LEFT_4_PENALTY=1.138774
2453
PRIMER_RIGHT_4_PENALTY=0.014419
2454
PRIMER_LEFT_4_SEQUENCE=CCTCATCCAGGCTTTCTTTC
2455
PRIMER_RIGHT_4_SEQUENCE=AGACTTGACCTCTGCAGGGA
2456
PRIMER_LEFT_4=61,20
2457
PRIMER_RIGHT_4=224,20
2458
PRIMER_LEFT_4_TM=58.861
2459
PRIMER_RIGHT_4_TM=59.986
2460
PRIMER_LEFT_4_GC_PERCENT=50.000
2461
PRIMER_RIGHT_4_GC_PERCENT=55.000
2462
PRIMER_LEFT_4_SELF_ANY=3.00
2463
PRIMER_RIGHT_4_SELF_ANY=6.00
2464
PRIMER_LEFT_4_SELF_END=0.00
2465
PRIMER_RIGHT_4_SELF_END=4.00
2466
PRIMER_LEFT_4_END_STABILITY=7.0000
2467
PRIMER_RIGHT_4_END_STABILITY=9.4000
2468
PRIMER_PAIR_4_COMPL_ANY=4.00
2469
PRIMER_PAIR_4_COMPL_END=2.00
2470
PRIMER_PRODUCT_SIZE_4=164
2471
=
2472
MARKER_NAME=24.RHAP33FB12.seq
2473
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCTTTTAGGTGTATAGGTTACATNAGCAAGGTTNCAGTTTAAACTTATTGGTTAGCATATTGACCTTTGAATCTGCTGGCTAGCAAGCATGGACAGATTATGACATGCCTTGGAACTCAATCTGGGACCAAAGGTTCAGGTGACTGTCAGCCAGTACTTNNNNNNNNNNNNNNNNNNNNNNNNNTNTNNNNNNNNNNNNNNTCTCTCNGCGTCNCNNCCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTTTTNTGTGTGTNTGTTTTTTTNNGNNTATGTNNGGCAGCNNTATNGGNATTTGGGNNTANCGCTTTNNGACNNAANNGNGNCCNNNAGTGNAC
2474
INCLUDED_REGION=23,356
2475
TARGET=181,22,TC
2476
TARGET=209,14,NC
2477
TARGET=243,40,TG
2478
PRIMER_COMMENT=CL is
2479
PRIMER_COMMENT=CR is
2480
=
2481
MARKER_NAME=24.RHAP34FB12.seq
2482
SEQUENCE=NNNGGGAGNTAAGCATGGAATCAAATCTGACAATAACTAACTAACAATAGTTGNCCTACCAAGTTGNAACCAAGCAACCATTTTGCTGAAAAANTGCTNTCTTTTTTCCANTTGANNTGGTTTTGAGACNTCCNNTANGTCAAAAAAAAATCNAGTGAANCCANTANGAGANGNTAGANGAGAGATATCANTANTCNTAGAGAGNTACTATACAAATATACATANTAACAAAAATAAGAGATACATAATCATAGNTACATAGATACATACATAGNTAACACATAAAAATAGAAAATAGAAACAATATATAGAAAGAATATATAAATNNATATATATAGAGACACNNNNNNNNNNNNNNAAACAAATATNCNTAATAGATAGNAAAGACNGNCAGACAGACCCANNNCNNAACNNNCTNTCTAACTACAAAC
2483
INCLUDED_REGION=3,428
2484
TARGET=344,14,AT
2485
PRIMER_COMMENT=CL is
2486
PRIMER_COMMENT=CR is
2487
=
2488
MARKER_NAME=26.RHAP33FC2.seq
2489
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCTCGGCACATTTTTAAAATAGATTCAATCATTCAAATTCAATCACAAATTTTCTACCCCATAATAATTTCTAATCATTTCACAGTGCACACACTCCTGCATCTAAATGCCTTCTTCCACATGTCAAAATAGAATTCATGTCTATTACCACACCTCTGAACTATCAAATCATTCAAAATGAAGATCATAAAATGAAGATGCATGGAAATGATGTGTAATGCCATAATAGGATAGCTACAAGAGTAACAGCAGGAGTAGCCAGTCCTTTGGAGAGTCATATTTAACCATGTGCTAAATAAGAGTTAGCAATATAATGCTGTAAGGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAC
2490
INCLUDED_REGION=23,361
2491
TARGET=348,33,AC
2492
PRIMER_COMMENT=CL is
2493
PRIMER_COMMENT=CR is
2494
=
2495
MARKER_NAME=26.RHAP34FG2.seq
2496
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTGTTAGATAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAACCTGCTACTTAGAATCAGGAGCCACAGAACAGTGCAGTTCAGGAAACATCGGTTGACTTGATCATGAAGTCCTTAGAAGGGATGGAACCCACAGAACTGAAGATGTCCACAGAGAACNNTAGATAGACCATACTGAGGAACTCTGACATATCACTATCTACTGACATNTGATATATGAAGAGTATGACCACACAATCATATTATCATAGACNNTAATATATAGAAAAGNTTAGATNNCACAGAGATATATATCATGACTNTT
2497
INCLUDED_REGION=29,334
2498
TARGET=43,54,GT
2499
PRIMER_COMMENT=CL is
2500
PRIMER_COMMENT=CR is
2501
=
2502
MARKER_NAME=27.RHAP33FC3.seq
2503
SEQUENCE=NNNNNNNNNNNCTGCATTTTCTAGAAAGCATAACTAAAAGTACATGGGATCCTTGTGCGTGGCAGAGACATAATCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGAGAGAATANNNNNNNNNNNNNNNNNNNNAGTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGANAGAGCANNNNNNNNNNNNNNNAGACCTGANTGANNNNNNNNNNNNNNNNNNNNNNCCAANGCTGGACANAGTGANATCAGTNCCCCNNACATNAC
2504
INCLUDED_REGION=11,358
2505
TARGET=77,58,GT
2506
TARGET=146,20,TG
2507
TARGET=171,38,GT
2508
TARGET=217,54,GT
2509
TARGET=281,14,TG
2510
TARGET=307,22,GT
2511
PRIMER_COMMENT=CL is
2512
PRIMER_COMMENT=CR is
2513
=
2514
MARKER_NAME=27.RHAP33FG3.seq
2515
SEQUENCE=NNNNNNNNNNNNNNNNCTAGGCAATGGATGGACTGGCACGGAGGTCTAATGCACAGAGCCACACTGCTAGAGAAGTCTGACCTAAAAAGTCAGTCAAATTAATAAATGAGAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGGGGGAGGGGCTATGGGAATCTAAGAAACACAGTTTAAAATTGCNTTTAAATTAAATTTTTAGTTAGTATACCAAATAGCATTTTATTATGACAGTTTTGTNTACNNNNNNNNNNNNNNNNNNNNNNNNCTATGTATGTATATATAATTGAANTTGGCCTNTTATTTGNCCCCCCANNNCCCCCCNCAAGGNATANA
2516
INCLUDED_REGION=16,365
2517
TARGET=114,68,GT
2518
TARGET=289,24,AT
2519
PRIMER_COMMENT=CL is GCACAGAGCCACACTGCTAG
2520
PRIMER_COMMENT=CR is TAGATTCCCATAGCCCCTCC
2521
PRIMER_PAIR_PENALTY=0.4436
2522
PRIMER_LEFT_PENALTY=0.198986
2523
PRIMER_RIGHT_PENALTY=0.244611
2524
PRIMER_LEFT_SEQUENCE=GCACAGAGCCACACTGCTAG
2525
PRIMER_RIGHT_SEQUENCE=TAGATTCCCATAGCCCCTCC
2526
PRIMER_LEFT=50,20
2527
PRIMER_RIGHT=206,20
2528
PRIMER_LEFT_TM=59.801
2529
PRIMER_RIGHT_TM=60.245
2530
PRIMER_LEFT_GC_PERCENT=60.000
2531
PRIMER_RIGHT_GC_PERCENT=55.000
2532
PRIMER_LEFT_SELF_ANY=4.00
2533
PRIMER_RIGHT_SELF_ANY=3.00
2534
PRIMER_LEFT_SELF_END=4.00
2535
PRIMER_RIGHT_SELF_END=0.00
2536
PRIMER_LEFT_END_STABILITY=7.2000
2537
PRIMER_RIGHT_END_STABILITY=9.4000
2538
PRIMER_PAIR_COMPL_ANY=4.00
2539
PRIMER_PAIR_COMPL_END=1.00
2540
PRIMER_PRODUCT_SIZE=157
2541
PRIMER_PAIR_PENALTY_1=0.9309
2542
PRIMER_LEFT_1_PENALTY=0.686256
2543
PRIMER_RIGHT_1_PENALTY=0.244611
2544
PRIMER_LEFT_1_SEQUENCE=CACGGAGGTCTAATGCACAG
2545
PRIMER_RIGHT_1_SEQUENCE=TAGATTCCCATAGCCCCTCC
2546
PRIMER_LEFT_1=36,20
2547
PRIMER_RIGHT_1=206,20
2548
PRIMER_LEFT_1_TM=59.314
2549
PRIMER_RIGHT_1_TM=60.245
2550
PRIMER_LEFT_1_GC_PERCENT=55.000
2551
PRIMER_RIGHT_1_GC_PERCENT=55.000
2552
PRIMER_LEFT_1_SELF_ANY=4.00
2553
PRIMER_RIGHT_1_SELF_ANY=3.00
2554
PRIMER_LEFT_1_SELF_END=2.00
2555
PRIMER_RIGHT_1_SELF_END=0.00
2556
PRIMER_LEFT_1_END_STABILITY=6.7000
2557
PRIMER_RIGHT_1_END_STABILITY=9.4000
2558
PRIMER_PAIR_1_COMPL_ANY=5.00
2559
PRIMER_PAIR_1_COMPL_END=2.00
2560
PRIMER_PRODUCT_SIZE_1=171
2561
PRIMER_PAIR_PENALTY_2=1.3769
2562
PRIMER_LEFT_2_PENALTY=0.133741
2563
PRIMER_RIGHT_2_PENALTY=1.243148
2564
PRIMER_LEFT_2_SEQUENCE=ACTGGCACGGAGGTCTAATG
2565
PRIMER_RIGHT_2_SEQUENCE=AGATTCCCATAGCCCCTCC
2566
PRIMER_LEFT_2=31,20
2567
PRIMER_RIGHT_2=205,19
2568
PRIMER_LEFT_2_TM=60.134
2569
PRIMER_RIGHT_2_TM=60.243
2570
PRIMER_LEFT_2_GC_PERCENT=55.000
2571
PRIMER_RIGHT_2_GC_PERCENT=57.895
2572
PRIMER_LEFT_2_SELF_ANY=3.00
2573
PRIMER_RIGHT_2_SELF_ANY=3.00
2574
PRIMER_LEFT_2_SELF_END=0.00
2575
PRIMER_RIGHT_2_SELF_END=0.00
2576
PRIMER_LEFT_2_END_STABILITY=6.2000
2577
PRIMER_RIGHT_2_END_STABILITY=9.4000
2578
PRIMER_PAIR_2_COMPL_ANY=5.00
2579
PRIMER_PAIR_2_COMPL_END=5.00
2580
PRIMER_PRODUCT_SIZE_2=175
2581
PRIMER_PAIR_PENALTY_3=1.4421
2582
PRIMER_LEFT_3_PENALTY=0.198986
2583
PRIMER_RIGHT_3_PENALTY=1.243148
2584
PRIMER_LEFT_3_SEQUENCE=GCACAGAGCCACACTGCTAG
2585
PRIMER_RIGHT_3_SEQUENCE=AGATTCCCATAGCCCCTCC
2586
PRIMER_LEFT_3=50,20
2587
PRIMER_RIGHT_3=205,19
2588
PRIMER_LEFT_3_TM=59.801
2589
PRIMER_RIGHT_3_TM=60.243
2590
PRIMER_LEFT_3_GC_PERCENT=60.000
2591
PRIMER_RIGHT_3_GC_PERCENT=57.895
2592
PRIMER_LEFT_3_SELF_ANY=4.00
2593
PRIMER_RIGHT_3_SELF_ANY=3.00
2594
PRIMER_LEFT_3_SELF_END=4.00
2595
PRIMER_RIGHT_3_SELF_END=0.00
2596
PRIMER_LEFT_3_END_STABILITY=7.2000
2597
PRIMER_RIGHT_3_END_STABILITY=9.4000
2598
PRIMER_PAIR_3_COMPL_ANY=4.00
2599
PRIMER_PAIR_3_COMPL_END=1.00
2600
PRIMER_PRODUCT_SIZE_3=156
2601
PRIMER_PAIR_PENALTY_4=1.6568
2602
PRIMER_LEFT_4_PENALTY=1.412222
2603
PRIMER_RIGHT_4_PENALTY=0.244611
2604
PRIMER_LEFT_4_SEQUENCE=GAGGTCTAATGCACAGAGCCA
2605
PRIMER_RIGHT_4_SEQUENCE=TAGATTCCCATAGCCCCTCC
2606
PRIMER_LEFT_4=40,21
2607
PRIMER_RIGHT_4=206,20
2608
PRIMER_LEFT_4_TM=60.412
2609
PRIMER_RIGHT_4_TM=60.245
2610
PRIMER_LEFT_4_GC_PERCENT=52.381
2611
PRIMER_RIGHT_4_GC_PERCENT=55.000
2612
PRIMER_LEFT_4_SELF_ANY=4.00
2613
PRIMER_RIGHT_4_SELF_ANY=3.00
2614
PRIMER_LEFT_4_SELF_END=2.00
2615
PRIMER_RIGHT_4_SELF_END=0.00
2616
PRIMER_LEFT_4_END_STABILITY=9.7000
2617
PRIMER_RIGHT_4_END_STABILITY=9.4000
2618
PRIMER_PAIR_4_COMPL_ANY=4.00
2619
PRIMER_PAIR_4_COMPL_END=0.00
2620
PRIMER_PRODUCT_SIZE_4=167
2621
=
2622
MARKER_NAME=28.RHAP33FC4.seq
2623
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNCTGTCCAACAGCCTGGCTGGATAGGTGAGGTCCAGCCTCAATATGAGATCCTGCCTCAAAAAATAAAGCATGATAGAGGAAGACACATAGACAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTTCTCAAAATTCTCTATTCCTTTAAACATATTCTCAGTCATCAGTAAAAGAAAATTTCTATTTATTAATAAGTTAGTAGGAATTATTTTGTTGCTAGTAGATTTTGATACAGACTTGTGTTGTCCAGGTTAGTCTTAAACTGGTCTCACATATTTTACTGCAGTCTGAAGTTACAGGCCTGTGTCANTGTGNNTGGCTTACAGNATTTGCT
2624
INCLUDED_REGION=22,359
2625
TARGET=117,50,CA
2626
PRIMER_COMMENT=CL is GGTGAGGTCCAGCCTCAATA
2627
PRIMER_COMMENT=CR is GGCCTGTAACTTCAGACTGCA
2628
PRIMER_PAIR_PENALTY=1.5196
2629
PRIMER_LEFT_PENALTY=0.072951
2630
PRIMER_RIGHT_PENALTY=1.446651
2631
PRIMER_LEFT_SEQUENCE=GGTGAGGTCCAGCCTCAATA
2632
PRIMER_RIGHT_SEQUENCE=GGCCTGTAACTTCAGACTGCA
2633
PRIMER_LEFT=45,20
2634
PRIMER_RIGHT=348,21
2635
PRIMER_LEFT_TM=60.073
2636
PRIMER_RIGHT_TM=60.447
2637
PRIMER_LEFT_GC_PERCENT=55.000
2638
PRIMER_RIGHT_GC_PERCENT=52.381
2639
PRIMER_LEFT_SELF_ANY=6.00
2640
PRIMER_RIGHT_SELF_ANY=5.00
2641
PRIMER_LEFT_SELF_END=2.00
2642
PRIMER_RIGHT_SELF_END=5.00
2643
PRIMER_LEFT_END_STABILITY=6.2000
2644
PRIMER_RIGHT_END_STABILITY=8.5000
2645
PRIMER_PAIR_COMPL_ANY=5.00
2646
PRIMER_PAIR_COMPL_END=3.00
2647
PRIMER_PRODUCT_SIZE=304
2648
PRIMER_PAIR_PENALTY_1=1.7234
2649
PRIMER_LEFT_1_PENALTY=0.072951
2650
PRIMER_RIGHT_1_PENALTY=1.650466
2651
PRIMER_LEFT_1_SEQUENCE=GGTGAGGTCCAGCCTCAATA
2652
PRIMER_RIGHT_1_SEQUENCE=TGACACAGGCCTGTAACTTCA
2653
PRIMER_LEFT_1=45,20
2654
PRIMER_RIGHT_1=355,21
2655
PRIMER_LEFT_1_TM=60.073
2656
PRIMER_RIGHT_1_TM=59.350
2657
PRIMER_LEFT_1_GC_PERCENT=55.000
2658
PRIMER_RIGHT_1_GC_PERCENT=47.619
2659
PRIMER_LEFT_1_SELF_ANY=6.00
2660
PRIMER_RIGHT_1_SELF_ANY=10.00
2661
PRIMER_LEFT_1_SELF_END=2.00
2662
PRIMER_RIGHT_1_SELF_END=4.00
2663
PRIMER_LEFT_1_END_STABILITY=6.2000
2664
PRIMER_RIGHT_1_END_STABILITY=7.0000
2665
PRIMER_PAIR_1_COMPL_ANY=5.00
2666
PRIMER_PAIR_1_COMPL_END=2.00
2667
PRIMER_PRODUCT_SIZE_1=311
2668
PRIMER_PAIR_PENALTY_2=1.7676
2669
PRIMER_LEFT_2_PENALTY=0.320904
2670
PRIMER_RIGHT_2_PENALTY=1.446651
2671
PRIMER_LEFT_2_SEQUENCE=CTGGCTGGATAGGTGAGGTC
2672
PRIMER_RIGHT_2_SEQUENCE=GGCCTGTAACTTCAGACTGCA
2673
PRIMER_LEFT_2=34,20
2674
PRIMER_RIGHT_2=348,21
2675
PRIMER_LEFT_2_TM=59.679
2676
PRIMER_RIGHT_2_TM=60.447
2677
PRIMER_LEFT_2_GC_PERCENT=60.000
2678
PRIMER_RIGHT_2_GC_PERCENT=52.381
2679
PRIMER_LEFT_2_SELF_ANY=2.00
2680
PRIMER_RIGHT_2_SELF_ANY=5.00
2681
PRIMER_LEFT_2_SELF_END=2.00
2682
PRIMER_RIGHT_2_SELF_END=5.00
2683
PRIMER_LEFT_2_END_STABILITY=7.6000
2684
PRIMER_RIGHT_2_END_STABILITY=8.5000
2685
PRIMER_PAIR_2_COMPL_ANY=4.00
2686
PRIMER_PAIR_2_COMPL_END=3.00
2687
PRIMER_PRODUCT_SIZE_2=315
2688
PRIMER_PAIR_PENALTY_3=1.7676
2689
PRIMER_LEFT_3_PENALTY=0.320904
2690
PRIMER_RIGHT_3_PENALTY=1.446651
2691
PRIMER_LEFT_3_SEQUENCE=CTGGATAGGTGAGGTCCAGC
2692
PRIMER_RIGHT_3_SEQUENCE=GGCCTGTAACTTCAGACTGCA
2693
PRIMER_LEFT_3=38,20
2694
PRIMER_RIGHT_3=348,21
2695
PRIMER_LEFT_3_TM=59.679
2696
PRIMER_RIGHT_3_TM=60.447
2697
PRIMER_LEFT_3_GC_PERCENT=60.000
2698
PRIMER_RIGHT_3_GC_PERCENT=52.381
2699
PRIMER_LEFT_3_SELF_ANY=5.00
2700
PRIMER_RIGHT_3_SELF_ANY=5.00
2701
PRIMER_LEFT_3_SELF_END=2.00
2702
PRIMER_RIGHT_3_SELF_END=5.00
2703
PRIMER_LEFT_3_END_STABILITY=9.7000
2704
PRIMER_RIGHT_3_END_STABILITY=8.5000
2705
PRIMER_PAIR_3_COMPL_ANY=4.00
2706
PRIMER_PAIR_3_COMPL_END=2.00
2707
PRIMER_PRODUCT_SIZE_3=311
2708
PRIMER_PAIR_PENALTY_4=1.7676
2709
PRIMER_LEFT_4_PENALTY=0.320904
2710
PRIMER_RIGHT_4_PENALTY=1.446651
2711
PRIMER_LEFT_4_SEQUENCE=GCTGGATAGGTGAGGTCCAG
2712
PRIMER_RIGHT_4_SEQUENCE=GGCCTGTAACTTCAGACTGCA
2713
PRIMER_LEFT_4=37,20
2714
PRIMER_RIGHT_4=348,21
2715
PRIMER_LEFT_4_TM=59.679
2716
PRIMER_RIGHT_4_TM=60.447
2717
PRIMER_LEFT_4_GC_PERCENT=60.000
2718
PRIMER_RIGHT_4_GC_PERCENT=52.381
2719
PRIMER_LEFT_4_SELF_ANY=5.00
2720
PRIMER_RIGHT_4_SELF_ANY=5.00
2721
PRIMER_LEFT_4_SELF_END=1.00
2722
PRIMER_RIGHT_4_SELF_END=5.00
2723
PRIMER_LEFT_4_END_STABILITY=8.2000
2724
PRIMER_RIGHT_4_END_STABILITY=8.5000
2725
PRIMER_PAIR_4_COMPL_ANY=4.00
2726
PRIMER_PAIR_4_COMPL_END=3.00
2727
PRIMER_PRODUCT_SIZE_4=312
2728
=
2729
MARKER_NAME=28.RHAP34FC4.seq
2730
SEQUENCE=NNNNNNNNNNNNACAATAATATATGAGTTGTTCCTTTAATCATGAATCATATTTCCTAGTATATTTTGAAACAATATTATTCATATATATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGTATCTTCAAATGTACTGAATATTTCATCTATCACTTCTAATACTGAGGAAGCACCCAAGCACTCACCTTCAGAGAGGCAAAAGTGTTAGGGAAAGCACATTTCTTCTTCTTAAATACAT
2731
INCLUDED_REGION=12,274
2732
TARGET=91,72,TG
2733
PRIMER_COMMENT=CL is GAGTTGTTCCTTTAATCATGAATCA
2734
PRIMER_COMMENT=CR is GCCTCTCTGAAGGTGAGTGC
2735
PRIMER_PAIR_PENALTY=6.5213
2736
PRIMER_LEFT_PENALTY=6.377880
2737
PRIMER_RIGHT_PENALTY=0.143403
2738
PRIMER_LEFT_SEQUENCE=TGAGTTGTTCCTTTAATCATGAATC
2739
PRIMER_RIGHT_SEQUENCE=GCCTCTCTGAAGGTGAGTGC
2740
PRIMER_LEFT=23,25
2741
PRIMER_RIGHT=244,20
2742
PRIMER_LEFT_TM=58.622
2743
PRIMER_RIGHT_TM=60.143
2744
PRIMER_LEFT_GC_PERCENT=32.000
2745
PRIMER_RIGHT_GC_PERCENT=60.000
2746
PRIMER_LEFT_SELF_ANY=6.00
2747
PRIMER_RIGHT_SELF_ANY=5.00
2748
PRIMER_LEFT_SELF_END=4.00
2749
PRIMER_RIGHT_SELF_END=2.00
2750
PRIMER_LEFT_END_STABILITY=6.6000
2751
PRIMER_RIGHT_END_STABILITY=7.9000
2752
PRIMER_PAIR_COMPL_ANY=4.00
2753
PRIMER_PAIR_COMPL_END=1.00
2754
PRIMER_PRODUCT_SIZE=222
2755
PRIMER_PAIR_PENALTY_1=6.5213
2756
PRIMER_LEFT_1_PENALTY=6.377880
2757
PRIMER_RIGHT_1_PENALTY=0.143403
2758
PRIMER_LEFT_1_SEQUENCE=GAGTTGTTCCTTTAATCATGAATCA
2759
PRIMER_RIGHT_1_SEQUENCE=GCCTCTCTGAAGGTGAGTGC
2760
PRIMER_LEFT_1=24,25
2761
PRIMER_RIGHT_1=244,20
2762
PRIMER_LEFT_1_TM=58.622
2763
PRIMER_RIGHT_1_TM=60.143
2764
PRIMER_LEFT_1_GC_PERCENT=32.000
2765
PRIMER_RIGHT_1_GC_PERCENT=60.000
2766
PRIMER_LEFT_1_SELF_ANY=6.00
2767
PRIMER_RIGHT_1_SELF_ANY=5.00
2768
PRIMER_LEFT_1_SELF_END=6.00
2769
PRIMER_RIGHT_1_SELF_END=2.00
2770
PRIMER_LEFT_1_END_STABILITY=6.9000
2771
PRIMER_RIGHT_1_END_STABILITY=7.9000
2772
PRIMER_PAIR_1_COMPL_ANY=4.00
2773
PRIMER_PAIR_1_COMPL_END=2.00
2774
PRIMER_PRODUCT_SIZE_1=221
2775
PRIMER_PAIR_PENALTY_2=6.6759
2776
PRIMER_LEFT_2_PENALTY=6.377880
2777
PRIMER_RIGHT_2_PENALTY=0.298018
2778
PRIMER_LEFT_2_SEQUENCE=TGAGTTGTTCCTTTAATCATGAATC
2779
PRIMER_RIGHT_2_SEQUENCE=CTGAAGGTGAGTGCTTGGGT
2780
PRIMER_LEFT_2=23,25
2781
PRIMER_RIGHT_2=238,20
2782
PRIMER_LEFT_2_TM=58.622
2783
PRIMER_RIGHT_2_TM=60.298
2784
PRIMER_LEFT_2_GC_PERCENT=32.000
2785
PRIMER_RIGHT_2_GC_PERCENT=55.000
2786
PRIMER_LEFT_2_SELF_ANY=6.00
2787
PRIMER_RIGHT_2_SELF_ANY=3.00
2788
PRIMER_LEFT_2_SELF_END=4.00
2789
PRIMER_RIGHT_2_SELF_END=0.00
2790
PRIMER_LEFT_2_END_STABILITY=6.6000
2791
PRIMER_RIGHT_2_END_STABILITY=9.4000
2792
PRIMER_PAIR_2_COMPL_ANY=4.00
2793
PRIMER_PAIR_2_COMPL_END=1.00
2794
PRIMER_PRODUCT_SIZE_2=216
2795
PRIMER_PAIR_PENALTY_3=6.6759
2796
PRIMER_LEFT_3_PENALTY=6.377880
2797
PRIMER_RIGHT_3_PENALTY=0.298018
2798
PRIMER_LEFT_3_SEQUENCE=GAGTTGTTCCTTTAATCATGAATCA
2799
PRIMER_RIGHT_3_SEQUENCE=CTGAAGGTGAGTGCTTGGGT
2800
PRIMER_LEFT_3=24,25
2801
PRIMER_RIGHT_3=238,20
2802
PRIMER_LEFT_3_TM=58.622
2803
PRIMER_RIGHT_3_TM=60.298
2804
PRIMER_LEFT_3_GC_PERCENT=32.000
2805
PRIMER_RIGHT_3_GC_PERCENT=55.000
2806
PRIMER_LEFT_3_SELF_ANY=6.00
2807
PRIMER_RIGHT_3_SELF_ANY=3.00
2808
PRIMER_LEFT_3_SELF_END=6.00
2809
PRIMER_RIGHT_3_SELF_END=0.00
2810
PRIMER_LEFT_3_END_STABILITY=6.9000
2811
PRIMER_RIGHT_3_END_STABILITY=9.4000
2812
PRIMER_PAIR_3_COMPL_ANY=4.00
2813
PRIMER_PAIR_3_COMPL_END=2.00
2814
PRIMER_PRODUCT_SIZE_3=215
2815
PRIMER_PAIR_PENALTY_4=6.7748
2816
PRIMER_LEFT_4_PENALTY=6.377880
2817
PRIMER_RIGHT_4_PENALTY=0.396944
2818
PRIMER_LEFT_4_SEQUENCE=TGAGTTGTTCCTTTAATCATGAATC
2819
PRIMER_RIGHT_4_SEQUENCE=GCTTGGGTGCTTCCTCAGTA
2820
PRIMER_LEFT_4=23,25
2821
PRIMER_RIGHT_4=226,20
2822
PRIMER_LEFT_4_TM=58.622
2823
PRIMER_RIGHT_4_TM=60.397
2824
PRIMER_LEFT_4_GC_PERCENT=32.000
2825
PRIMER_RIGHT_4_GC_PERCENT=55.000
2826
PRIMER_LEFT_4_SELF_ANY=6.00
2827
PRIMER_RIGHT_4_SELF_ANY=3.00
2828
PRIMER_LEFT_4_SELF_END=4.00
2829
PRIMER_RIGHT_4_SELF_END=3.00
2830
PRIMER_LEFT_4_END_STABILITY=6.6000
2831
PRIMER_RIGHT_4_END_STABILITY=5.7000
2832
PRIMER_PAIR_4_COMPL_ANY=4.00
2833
PRIMER_PAIR_4_COMPL_END=4.00
2834
PRIMER_PRODUCT_SIZE_4=204
2835
=
2836
MARKER_NAME=28.RHAP34FG4.seq
2837
SEQUENCE=NNNNNNNNNNNNNNNNNNNNCCTGGTATCCTCCCTCTTTTGTCTTAGTTTAACCAGGAGAAACTCTCCCAAACTTTAACCTTATTAACAATTGCATGCAAAGTATGCCTGACTTAGGAATGTGGTGCAAAAGTCAGTCCCAAGGCGAGGAAATAACAGGTATTTTCTGAAGTCTTGTCTTAAGTCTGTTGTTGCAGCGGCCTTGAGTTTAATCCGTGGCACTGAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACGGGGCAAACTGGAATCTCAGGAA
2838
INCLUDED_REGION=20,280
2839
TARGET=233,42,GT
2840
PRIMER_COMMENT=CL is CAAGGCGAGGAAATAACAGG
2841
PRIMER_COMMENT=CR is TCCTGAGATTCCAGTTTGCC
2842
PRIMER_PAIR_PENALTY=0.4935
2843
PRIMER_LEFT_PENALTY=0.298125
2844
PRIMER_RIGHT_PENALTY=0.195358
2845
PRIMER_LEFT_SEQUENCE=CCAAGGCGAGGAAATAACAG
2846
PRIMER_RIGHT_SEQUENCE=TCCTGAGATTCCAGTTTGCC
2847
PRIMER_LEFT=138,20
2848
PRIMER_RIGHT=298,20
2849
PRIMER_LEFT_TM=59.702
2850
PRIMER_RIGHT_TM=60.195
2851
PRIMER_LEFT_GC_PERCENT=50.000
2852
PRIMER_RIGHT_GC_PERCENT=50.000
2853
PRIMER_LEFT_SELF_ANY=2.00
2854
PRIMER_RIGHT_SELF_ANY=5.00
2855
PRIMER_LEFT_SELF_END=1.00
2856
PRIMER_RIGHT_SELF_END=1.00
2857
PRIMER_LEFT_END_STABILITY=6.7000
2858
PRIMER_RIGHT_END_STABILITY=10.0000
2859
PRIMER_PAIR_COMPL_ANY=4.00
2860
PRIMER_PAIR_COMPL_END=2.00
2861
PRIMER_PRODUCT_SIZE=161
2862
PRIMER_PAIR_PENALTY_1=0.4935
2863
PRIMER_LEFT_1_PENALTY=0.298125
2864
PRIMER_RIGHT_1_PENALTY=0.195358
2865
PRIMER_LEFT_1_SEQUENCE=CAAGGCGAGGAAATAACAGG
2866
PRIMER_RIGHT_1_SEQUENCE=TCCTGAGATTCCAGTTTGCC
2867
PRIMER_LEFT_1=139,20
2868
PRIMER_RIGHT_1=298,20
2869
PRIMER_LEFT_1_TM=59.702
2870
PRIMER_RIGHT_1_TM=60.195
2871
PRIMER_LEFT_1_GC_PERCENT=50.000
2872
PRIMER_RIGHT_1_GC_PERCENT=50.000
2873
PRIMER_LEFT_1_SELF_ANY=2.00
2874
PRIMER_RIGHT_1_SELF_ANY=5.00
2875
PRIMER_LEFT_1_SELF_END=0.00
2876
PRIMER_RIGHT_1_SELF_END=1.00
2877
PRIMER_LEFT_1_END_STABILITY=7.9000
2878
PRIMER_RIGHT_1_END_STABILITY=10.0000
2879
PRIMER_PAIR_1_COMPL_ANY=5.00
2880
PRIMER_PAIR_1_COMPL_END=3.00
2881
PRIMER_PRODUCT_SIZE_1=160
2882
PRIMER_PAIR_PENALTY_2=1.1441
2883
PRIMER_LEFT_2_PENALTY=0.948713
2884
PRIMER_RIGHT_2_PENALTY=0.195358
2885
PRIMER_LEFT_2_SEQUENCE=AGTCCCAAGGCGAGGAAATA
2886
PRIMER_RIGHT_2_SEQUENCE=TCCTGAGATTCCAGTTTGCC
2887
PRIMER_LEFT_2=134,20
2888
PRIMER_RIGHT_2=298,20
2889
PRIMER_LEFT_2_TM=60.949
2890
PRIMER_RIGHT_2_TM=60.195
2891
PRIMER_LEFT_2_GC_PERCENT=50.000
2892
PRIMER_RIGHT_2_GC_PERCENT=50.000
2893
PRIMER_LEFT_2_SELF_ANY=3.00
2894
PRIMER_RIGHT_2_SELF_ANY=5.00
2895
PRIMER_LEFT_2_SELF_END=2.00
2896
PRIMER_RIGHT_2_SELF_END=1.00
2897
PRIMER_LEFT_2_END_STABILITY=6.2000
2898
PRIMER_RIGHT_2_END_STABILITY=10.0000
2899
PRIMER_PAIR_2_COMPL_ANY=4.00
2900
PRIMER_PAIR_2_COMPL_END=2.00
2901
PRIMER_PRODUCT_SIZE_2=165
2902
PRIMER_PAIR_PENALTY_3=1.2167
2903
PRIMER_LEFT_3_PENALTY=1.021310
2904
PRIMER_RIGHT_3_PENALTY=0.195358
2905
PRIMER_LEFT_3_SEQUENCE=GTCCCAAGGCGAGGAAATA
2906
PRIMER_RIGHT_3_SEQUENCE=TCCTGAGATTCCAGTTTGCC
2907
PRIMER_LEFT_3=135,19
2908
PRIMER_RIGHT_3=298,20
2909
PRIMER_LEFT_3_TM=60.021
2910
PRIMER_RIGHT_3_TM=60.195
2911
PRIMER_LEFT_3_GC_PERCENT=52.632
2912
PRIMER_RIGHT_3_GC_PERCENT=50.000
2913
PRIMER_LEFT_3_SELF_ANY=3.00
2914
PRIMER_RIGHT_3_SELF_ANY=5.00
2915
PRIMER_LEFT_3_SELF_END=2.00
2916
PRIMER_RIGHT_3_SELF_END=1.00
2917
PRIMER_LEFT_3_END_STABILITY=6.2000
2918
PRIMER_RIGHT_3_END_STABILITY=10.0000
2919
PRIMER_PAIR_3_COMPL_ANY=4.00
2920
PRIMER_PAIR_3_COMPL_END=2.00
2921
PRIMER_PRODUCT_SIZE_3=164
2922
PRIMER_PAIR_PENALTY_4=1.3993
2923
PRIMER_LEFT_4_PENALTY=1.203901
2924
PRIMER_RIGHT_4_PENALTY=0.195358
2925
PRIMER_LEFT_4_SEQUENCE=TGCAAAAGTCAGTCCCAAGG
2926
PRIMER_RIGHT_4_SEQUENCE=TCCTGAGATTCCAGTTTGCC
2927
PRIMER_LEFT_4=124,20
2928
PRIMER_RIGHT_4=298,20
2929
PRIMER_LEFT_4_TM=61.204
2930
PRIMER_RIGHT_4_TM=60.195
2931
PRIMER_LEFT_4_GC_PERCENT=50.000
2932
PRIMER_RIGHT_4_GC_PERCENT=50.000
2933
PRIMER_LEFT_4_SELF_ANY=4.00
2934
PRIMER_RIGHT_4_SELF_ANY=5.00
2935
PRIMER_LEFT_4_SELF_END=2.00
2936
PRIMER_RIGHT_4_SELF_END=1.00
2937
PRIMER_LEFT_4_END_STABILITY=8.5000
2938
PRIMER_RIGHT_4_END_STABILITY=10.0000
2939
PRIMER_PAIR_4_COMPL_ANY=5.00
2940
PRIMER_PAIR_4_COMPL_END=3.00
2941
PRIMER_PRODUCT_SIZE_4=175
2942
=
2943
MARKER_NAME=29.RHAP33FG5.seq
2944
SEQUENCE=NNNNNNNNNNNNNNCTAATGCAGCATTGATTTCTGAAGNNNNNNNNNNNNNNATTTGGGCTTCTGATAATTGTTTGGTTTGCCTATTGCCTTAGAATTAGTGTCGATTACGACCAACAGGAAAACTTCAAAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTTTTCNCACATGGACCTNTTTGTTTGAAAGTCCCTGCNGCTGCTTTGGACTGTGTGGATANNGTGCTCCACCTAGNCAGTTTGTGTGTATGGCATGNATATGCANCACACACACCGTGGTGCNTGGNTGNCCTGTGNAGGANAANAGCAGGGATNTTANATT
2945
INCLUDED_REGION=14,371
2946
TARGET=38,14,AC
2947
TARGET=133,87,TG
2948
PRIMER_COMMENT=CL is CGACCAACAGGAAAACTTCAA
2949
PRIMER_COMMENT=CR is ATCCACACAGTCCAAAGCAG
2950
PRIMER_PAIR_PENALTY=2.4073
2951
PRIMER_LEFT_PENALTY=1.131978
2952
PRIMER_RIGHT_PENALTY=1.275356
2953
PRIMER_LEFT_SEQUENCE=CGACCAACAGGAAAACTTCAA
2954
PRIMER_RIGHT_SEQUENCE=ATCCACACAGTCCAAAGCAG
2955
PRIMER_LEFT=109,21
2956
PRIMER_RIGHT=281,20
2957
PRIMER_LEFT_TM=60.132
2958
PRIMER_RIGHT_TM=58.725
2959
PRIMER_LEFT_GC_PERCENT=42.857
2960
PRIMER_RIGHT_GC_PERCENT=50.000
2961
PRIMER_LEFT_SELF_ANY=3.00
2962
PRIMER_RIGHT_SELF_ANY=3.00
2963
PRIMER_LEFT_SELF_END=3.00
2964
PRIMER_RIGHT_SELF_END=1.00
2965
PRIMER_LEFT_END_STABILITY=7.3000
2966
PRIMER_RIGHT_END_STABILITY=8.2000
2967
PRIMER_PAIR_COMPL_ANY=3.00
2968
PRIMER_PAIR_COMPL_END=0.00
2969
PRIMER_PRODUCT_SIZE=173
2970
PRIMER_PAIR_PENALTY_1=2.5291
2971
PRIMER_LEFT_1_PENALTY=1.253711
2972
PRIMER_RIGHT_1_PENALTY=1.275356
2973
PRIMER_LEFT_1_SEQUENCE=CGACCAACAGGAAAACTTCA
2974
PRIMER_RIGHT_1_SEQUENCE=ATCCACACAGTCCAAAGCAG
2975
PRIMER_LEFT_1=109,20
2976
PRIMER_RIGHT_1=281,20
2977
PRIMER_LEFT_1_TM=58.746
2978
PRIMER_RIGHT_1_TM=58.725
2979
PRIMER_LEFT_1_GC_PERCENT=45.000
2980
PRIMER_RIGHT_1_GC_PERCENT=50.000
2981
PRIMER_LEFT_1_SELF_ANY=3.00
2982
PRIMER_RIGHT_1_SELF_ANY=3.00
2983
PRIMER_LEFT_1_SELF_END=1.00
2984
PRIMER_RIGHT_1_SELF_END=1.00
2985
PRIMER_LEFT_1_END_STABILITY=7.0000
2986
PRIMER_RIGHT_1_END_STABILITY=8.2000
2987
PRIMER_PAIR_1_COMPL_ANY=3.00
2988
PRIMER_PAIR_1_COMPL_END=1.00
2989
PRIMER_PRODUCT_SIZE_1=173
2990
PRIMER_PAIR_PENALTY_2=2.5886
2991
PRIMER_LEFT_2_PENALTY=1.131978
2992
PRIMER_RIGHT_2_PENALTY=1.456605
2993
PRIMER_LEFT_2_SEQUENCE=CGACCAACAGGAAAACTTCAA
2994
PRIMER_RIGHT_2_SEQUENCE=CCACACAGTCCAAAGCAGC
2995
PRIMER_LEFT_2=109,21
2996
PRIMER_RIGHT_2=279,19
2997
PRIMER_LEFT_2_TM=60.132
2998
PRIMER_RIGHT_2_TM=60.457
2999
PRIMER_LEFT_2_GC_PERCENT=42.857
3000
PRIMER_RIGHT_2_GC_PERCENT=57.895
3001
PRIMER_LEFT_2_SELF_ANY=3.00
3002
PRIMER_RIGHT_2_SELF_ANY=3.00
3003
PRIMER_LEFT_2_SELF_END=3.00
3004
PRIMER_RIGHT_2_SELF_END=3.00
3005
PRIMER_LEFT_2_END_STABILITY=7.3000
3006
PRIMER_RIGHT_2_END_STABILITY=9.7000
3007
PRIMER_PAIR_2_COMPL_ANY=3.00
3008
PRIMER_PAIR_2_COMPL_END=0.00
3009
PRIMER_PRODUCT_SIZE_2=171
3010
PRIMER_PAIR_PENALTY_3=2.6510
3011
PRIMER_LEFT_3_PENALTY=1.375627
3012
PRIMER_RIGHT_3_PENALTY=1.275356
3013
PRIMER_LEFT_3_SEQUENCE=ACGACCAACAGGAAAACTTCA
3014
PRIMER_RIGHT_3_SEQUENCE=ATCCACACAGTCCAAAGCAG
3015
PRIMER_LEFT_3=108,21
3016
PRIMER_RIGHT_3=281,20
3017
PRIMER_LEFT_3_TM=59.624
3018
PRIMER_RIGHT_3_TM=58.725
3019
PRIMER_LEFT_3_GC_PERCENT=42.857
3020
PRIMER_RIGHT_3_GC_PERCENT=50.000
3021
PRIMER_LEFT_3_SELF_ANY=3.00
3022
PRIMER_RIGHT_3_SELF_ANY=3.00
3023
PRIMER_LEFT_3_SELF_END=1.00
3024
PRIMER_RIGHT_3_SELF_END=1.00
3025
PRIMER_LEFT_3_END_STABILITY=7.0000
3026
PRIMER_RIGHT_3_END_STABILITY=8.2000
3027
PRIMER_PAIR_3_COMPL_ANY=3.00
3028
PRIMER_PAIR_3_COMPL_END=1.00
3029
PRIMER_PRODUCT_SIZE_3=174
3030
PRIMER_PAIR_PENALTY_4=2.7103
3031
PRIMER_LEFT_4_PENALTY=1.253711
3032
PRIMER_RIGHT_4_PENALTY=1.456605
3033
PRIMER_LEFT_4_SEQUENCE=CGACCAACAGGAAAACTTCA
3034
PRIMER_RIGHT_4_SEQUENCE=CCACACAGTCCAAAGCAGC
3035
PRIMER_LEFT_4=109,20
3036
PRIMER_RIGHT_4=279,19
3037
PRIMER_LEFT_4_TM=58.746
3038
PRIMER_RIGHT_4_TM=60.457
3039
PRIMER_LEFT_4_GC_PERCENT=45.000
3040
PRIMER_RIGHT_4_GC_PERCENT=57.895
3041
PRIMER_LEFT_4_SELF_ANY=3.00
3042
PRIMER_RIGHT_4_SELF_ANY=3.00
3043
PRIMER_LEFT_4_SELF_END=1.00
3044
PRIMER_RIGHT_4_SELF_END=3.00
3045
PRIMER_LEFT_4_END_STABILITY=7.0000
3046
PRIMER_RIGHT_4_END_STABILITY=9.7000
3047
PRIMER_PAIR_4_COMPL_ANY=3.00
3048
PRIMER_PAIR_4_COMPL_END=0.00
3049
PRIMER_PRODUCT_SIZE_4=171
3050
=
3051
MARKER_NAME=30.RHAP34FC6.seq
3052
SEQUENCE=NNNNNNNNNNNNACCCACACGGGCACATTCAGCCGCANANATGAGNCNCAGNCGCACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNACAGCAACTTCTCACCAAACNATCCAAAACCTACNGACTTGAGGGGCNGTCCTTGTCGTAGCACTAAANGCANANGGCAGCAGGTGGACACTGGTGGTGTGGGCACCTTGNGTNNGAGAGNCGGGGGNNANGAGGNCCNGGAGGGGNGNNACCGANGCNTCGGNAAGNTTCGNNAANNCANNGGNTCANNAGNCGNGGNNNNCCNNGGGGGGGGNGNA
3053
INCLUDED_REGION=12,314
3054
TARGET=58,18,CT
3055
PRIMER_COMMENT=CL is CCACACGGGCACATTCAG
3056
PRIMER_COMMENT=CR is CACACCACCAGTGTCCACC
3057
PRIMER_PAIR_PENALTY=6.1728
3058
PRIMER_LEFT_PENALTY=4.227928
3059
PRIMER_RIGHT_PENALTY=1.944848
3060
PRIMER_LEFT_SEQUENCE=CCACACGGGCACATTCAG
3061
PRIMER_RIGHT_SEQUENCE=CACACCACCAGTGTCCACC
3062
PRIMER_LEFT=14,18
3063
PRIMER_RIGHT=208,19
3064
PRIMER_LEFT_TM=62.228
3065
PRIMER_RIGHT_TM=60.945
3066
PRIMER_LEFT_GC_PERCENT=61.111
3067
PRIMER_RIGHT_GC_PERCENT=63.158
3068
PRIMER_LEFT_SELF_ANY=2.00
3069
PRIMER_RIGHT_SELF_ANY=4.00
3070
PRIMER_LEFT_SELF_END=1.00
3071
PRIMER_RIGHT_SELF_END=2.00
3072
PRIMER_LEFT_END_STABILITY=7.0000
3073
PRIMER_RIGHT_END_STABILITY=9.4000
3074
PRIMER_PAIR_COMPL_ANY=5.00
3075
PRIMER_PAIR_COMPL_END=1.00
3076
PRIMER_PRODUCT_SIZE=195
3077
PRIMER_PAIR_PENALTY_1=6.1728
3078
PRIMER_LEFT_1_PENALTY=4.227928
3079
PRIMER_RIGHT_1_PENALTY=1.944848
3080
PRIMER_LEFT_1_SEQUENCE=CCACACGGGCACATTCAG
3081
PRIMER_RIGHT_1_SEQUENCE=CCACACCACCAGTGTCCAC
3082
PRIMER_LEFT_1=14,18
3083
PRIMER_RIGHT_1=209,19
3084
PRIMER_LEFT_1_TM=62.228
3085
PRIMER_RIGHT_1_TM=60.945
3086
PRIMER_LEFT_1_GC_PERCENT=61.111
3087
PRIMER_RIGHT_1_GC_PERCENT=63.158
3088
PRIMER_LEFT_1_SELF_ANY=2.00
3089
PRIMER_RIGHT_1_SELF_ANY=4.00
3090
PRIMER_LEFT_1_SELF_END=1.00
3091
PRIMER_RIGHT_1_SELF_END=4.00
3092
PRIMER_LEFT_1_END_STABILITY=7.0000
3093
PRIMER_RIGHT_1_END_STABILITY=7.9000
3094
PRIMER_PAIR_1_COMPL_ANY=5.00
3095
PRIMER_PAIR_1_COMPL_END=1.00
3096
PRIMER_PRODUCT_SIZE_1=196
3097
PRIMER_PAIR_PENALTY_2=6.2034
3098
PRIMER_LEFT_2_PENALTY=4.227928
3099
PRIMER_RIGHT_2_PENALTY=1.975514
3100
PRIMER_LEFT_2_SEQUENCE=CCACACGGGCACATTCAG
3101
PRIMER_RIGHT_2_SEQUENCE=CACACCACCAGTGTCCACCT
3102
PRIMER_LEFT_2=14,18
3103
PRIMER_RIGHT_2=208,20
3104
PRIMER_LEFT_2_TM=62.228
3105
PRIMER_RIGHT_2_TM=61.976
3106
PRIMER_LEFT_2_GC_PERCENT=61.111
3107
PRIMER_RIGHT_2_GC_PERCENT=60.000
3108
PRIMER_LEFT_2_SELF_ANY=2.00
3109
PRIMER_RIGHT_2_SELF_ANY=4.00
3110
PRIMER_LEFT_2_SELF_END=1.00
3111
PRIMER_RIGHT_2_SELF_END=2.00
3112
PRIMER_LEFT_2_END_STABILITY=7.0000
3113
PRIMER_RIGHT_2_END_STABILITY=7.9000
3114
PRIMER_PAIR_2_COMPL_ANY=5.00
3115
PRIMER_PAIR_2_COMPL_END=2.00
3116
PRIMER_PRODUCT_SIZE_2=195
3117
PRIMER_PAIR_PENALTY_3=6.2034
3118
PRIMER_LEFT_3_PENALTY=4.227928
3119
PRIMER_RIGHT_3_PENALTY=1.975514
3120
PRIMER_LEFT_3_SEQUENCE=CCACACGGGCACATTCAG
3121
PRIMER_RIGHT_3_SEQUENCE=ACACCACCAGTGTCCACCTG
3122
PRIMER_LEFT_3=14,18
3123
PRIMER_RIGHT_3=207,20
3124
PRIMER_LEFT_3_TM=62.228
3125
PRIMER_RIGHT_3_TM=61.976
3126
PRIMER_LEFT_3_GC_PERCENT=61.111
3127
PRIMER_RIGHT_3_GC_PERCENT=60.000
3128
PRIMER_LEFT_3_SELF_ANY=2.00
3129
PRIMER_RIGHT_3_SELF_ANY=4.00
3130
PRIMER_LEFT_3_SELF_END=1.00
3131
PRIMER_RIGHT_3_SELF_END=4.00
3132
PRIMER_LEFT_3_END_STABILITY=7.0000
3133
PRIMER_RIGHT_3_END_STABILITY=7.9000
3134
PRIMER_PAIR_3_COMPL_ANY=5.00
3135
PRIMER_PAIR_3_COMPL_END=3.00
3136
PRIMER_PRODUCT_SIZE_3=194
3137
PRIMER_PAIR_PENALTY_4=6.2688
3138
PRIMER_LEFT_4_PENALTY=4.227928
3139
PRIMER_RIGHT_4_PENALTY=2.040846
3140
PRIMER_LEFT_4_SEQUENCE=CCACACGGGCACATTCAG
3141
PRIMER_RIGHT_4_SEQUENCE=CCACACCACCAGTGTCCA
3142
PRIMER_LEFT_4=14,18
3143
PRIMER_RIGHT_4=209,18
3144
PRIMER_LEFT_4_TM=62.228
3145
PRIMER_RIGHT_4_TM=59.959
3146
PRIMER_LEFT_4_GC_PERCENT=61.111
3147
PRIMER_RIGHT_4_GC_PERCENT=61.111
3148
PRIMER_LEFT_4_SELF_ANY=2.00
3149
PRIMER_RIGHT_4_SELF_ANY=4.00
3150
PRIMER_LEFT_4_SELF_END=1.00
3151
PRIMER_RIGHT_4_SELF_END=2.00
3152
PRIMER_LEFT_4_END_STABILITY=7.0000
3153
PRIMER_RIGHT_4_END_STABILITY=7.9000
3154
PRIMER_PAIR_4_COMPL_ANY=5.00
3155
PRIMER_PAIR_4_COMPL_END=0.00
3156
PRIMER_PRODUCT_SIZE_4=196
3157
=
3158
MARKER_NAME=32.RHAP33FG8.seq
3159
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGTAGATGACAGGGTGCCCGCCTCCTGTGGGCGCAGGTGTGAGTCCTTGTAAGGCAACCTTGGGTTCCTGGCTGGACCTCAGAGGTTTCCCTCCATTGAGTGTGAGAGGGACTGTGGTAGTGGGGAAGGAACTGGTGGGCAGTGGAGGGTTCGCTCTTTAGACAGAGTACTACATACATAGAGAGTAAAGCAAAGAGCCGTAAAGAGGTGTGTTTGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGACTTGTGTGGGTACACACATGCATGCAGACCTGANGCCNTCNNCAATTACNTNC
3160
INCLUDED_REGION=29,362
3161
TARGET=248,35,TG
3162
TARGET=288,48,GT
3163
PRIMER_COMMENT=CL is AAGCAAAGAGCCGTAAAGAGG
3164
PRIMER_COMMENT=CR is AGGTCTGCATGCATGTGTGT
3165
PRIMER_PAIR_PENALTY=1.2238
3166
PRIMER_LEFT_PENALTY=1.028128
3167
PRIMER_RIGHT_PENALTY=0.195706
3168
PRIMER_LEFT_SEQUENCE=AAGCAAAGAGCCGTAAAGAGG
3169
PRIMER_RIGHT_SEQUENCE=AGGTCTGCATGCATGTGTGT
3170
PRIMER_LEFT=217,21
3171
PRIMER_RIGHT=368,20
3172
PRIMER_LEFT_TM=60.028
3173
PRIMER_RIGHT_TM=60.196
3174
PRIMER_LEFT_GC_PERCENT=47.619
3175
PRIMER_RIGHT_GC_PERCENT=50.000
3176
PRIMER_LEFT_SELF_ANY=2.00
3177
PRIMER_RIGHT_SELF_ANY=8.00
3178
PRIMER_LEFT_SELF_END=0.00
3179
PRIMER_RIGHT_SELF_END=2.00
3180
PRIMER_LEFT_END_STABILITY=7.9000
3181
PRIMER_RIGHT_END_STABILITY=6.4000
3182
PRIMER_PAIR_COMPL_ANY=3.00
3183
PRIMER_PAIR_COMPL_END=0.00
3184
PRIMER_PRODUCT_SIZE=152
3185
PRIMER_PAIR_PENALTY_1=1.4529
3186
PRIMER_LEFT_1_PENALTY=1.257158
3187
PRIMER_RIGHT_1_PENALTY=0.195706
3188
PRIMER_LEFT_1_SEQUENCE=AGCAAAGAGCCGTAAAGAGG
3189
PRIMER_RIGHT_1_SEQUENCE=AGGTCTGCATGCATGTGTGT
3190
PRIMER_LEFT_1=218,20
3191
PRIMER_RIGHT_1=368,20
3192
PRIMER_LEFT_1_TM=58.743
3193
PRIMER_RIGHT_1_TM=60.196
3194
PRIMER_LEFT_1_GC_PERCENT=50.000
3195
PRIMER_RIGHT_1_GC_PERCENT=50.000
3196
PRIMER_LEFT_1_SELF_ANY=2.00
3197
PRIMER_RIGHT_1_SELF_ANY=8.00
3198
PRIMER_LEFT_1_SELF_END=0.00
3199
PRIMER_RIGHT_1_SELF_END=2.00
3200
PRIMER_LEFT_1_END_STABILITY=7.9000
3201
PRIMER_RIGHT_1_END_STABILITY=6.4000
3202
PRIMER_PAIR_1_COMPL_ANY=3.00
3203
PRIMER_PAIR_1_COMPL_END=0.00
3204
PRIMER_PRODUCT_SIZE_1=151
3205
PRIMER_PAIR_PENALTY_2=1.5918
3206
PRIMER_LEFT_2_PENALTY=1.396076
3207
PRIMER_RIGHT_2_PENALTY=0.195706
3208
PRIMER_LEFT_2_SEQUENCE=GCAAAGAGCCGTAAAGAGGT
3209
PRIMER_RIGHT_2_SEQUENCE=AGGTCTGCATGCATGTGTGT
3210
PRIMER_LEFT_2=219,20
3211
PRIMER_RIGHT_2=368,20
3212
PRIMER_LEFT_2_TM=58.604
3213
PRIMER_RIGHT_2_TM=60.196
3214
PRIMER_LEFT_2_GC_PERCENT=50.000
3215
PRIMER_RIGHT_2_GC_PERCENT=50.000
3216
PRIMER_LEFT_2_SELF_ANY=2.00
3217
PRIMER_RIGHT_2_SELF_ANY=8.00
3218
PRIMER_LEFT_2_SELF_END=0.00
3219
PRIMER_RIGHT_2_SELF_END=2.00
3220
PRIMER_LEFT_2_END_STABILITY=7.6000
3221
PRIMER_RIGHT_2_END_STABILITY=6.4000
3222
PRIMER_PAIR_2_COMPL_ANY=3.00
3223
PRIMER_PAIR_2_COMPL_END=0.00
3224
PRIMER_PRODUCT_SIZE_2=150
3225
PRIMER_PAIR_PENALTY_3=1.6393
3226
PRIMER_LEFT_3_PENALTY=1.443584
3227
PRIMER_RIGHT_3_PENALTY=0.195706
3228
PRIMER_LEFT_3_SEQUENCE=AGCAAAGAGCCGTAAAGAGGT
3229
PRIMER_RIGHT_3_SEQUENCE=AGGTCTGCATGCATGTGTGT
3230
PRIMER_LEFT_3=218,21
3231
PRIMER_RIGHT_3=368,20
3232
PRIMER_LEFT_3_TM=59.556
3233
PRIMER_RIGHT_3_TM=60.196
3234
PRIMER_LEFT_3_GC_PERCENT=47.619
3235
PRIMER_RIGHT_3_GC_PERCENT=50.000
3236
PRIMER_LEFT_3_SELF_ANY=2.00
3237
PRIMER_RIGHT_3_SELF_ANY=8.00
3238
PRIMER_LEFT_3_SELF_END=0.00
3239
PRIMER_RIGHT_3_SELF_END=2.00
3240
PRIMER_LEFT_3_END_STABILITY=7.6000
3241
PRIMER_RIGHT_3_END_STABILITY=6.4000
3242
PRIMER_PAIR_3_COMPL_ANY=3.00
3243
PRIMER_PAIR_3_COMPL_END=0.00
3244
PRIMER_PRODUCT_SIZE_3=151
3245
PRIMER_PAIR_PENALTY_4=1.9005
3246
PRIMER_LEFT_4_PENALTY=1.704806
3247
PRIMER_RIGHT_4_PENALTY=0.195706
3248
PRIMER_LEFT_4_SEQUENCE=AGCCGTAAAGAGGTGTGTTTG
3249
PRIMER_RIGHT_4_SEQUENCE=AGGTCTGCATGCATGTGTGT
3250
PRIMER_LEFT_4=225,21
3251
PRIMER_RIGHT_4=368,20
3252
PRIMER_LEFT_4_TM=59.295
3253
PRIMER_RIGHT_4_TM=60.196
3254
PRIMER_LEFT_4_GC_PERCENT=47.619
3255
PRIMER_RIGHT_4_GC_PERCENT=50.000
3256
PRIMER_LEFT_4_SELF_ANY=3.00
3257
PRIMER_RIGHT_4_SELF_ANY=8.00
3258
PRIMER_LEFT_4_SELF_END=0.00
3259
PRIMER_RIGHT_4_SELF_END=2.00
3260
PRIMER_LEFT_4_END_STABILITY=7.0000
3261
PRIMER_RIGHT_4_END_STABILITY=6.4000
3262
PRIMER_PAIR_4_COMPL_ANY=3.00
3263
PRIMER_PAIR_4_COMPL_END=0.00
3264
PRIMER_PRODUCT_SIZE_4=144
3265
=
3266
MARKER_NAME=33.RHAP33FG9.seq
3267
SEQUENCE=NNNNNNNNNNNNNNNCTAGTCCAGTCAAATGGATGATCACTAGGTGCAAAGGGGGTGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTTGANAACAGCNTCCACCTAGAGCACNNNNNNNNNNNNNNACNAAGNAAACACATTTTCATTCCACACTTACATCATGCACACACATGCNTGCNGTGCATGNNNNNNNNNNNNNNNNCCCCCCCCANATAACNATAAAACCATACAGCAAACATNNTGTACAACCACCACNATNNNNNNNNNNNNNNTTTGTNTTCCGNTGGGCCAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
3268
INCLUDED_REGION=15,312
3269
TARGET=59,58,GA
3270
TARGET=145,14,AT
3271
TARGET=220,16,CA
3272
TARGET=292,14,CA
3273
PRIMER_COMMENT=CL is GATCACTAGGTGCAAAGGGG
3274
PRIMER_COMMENT=CR is GCATGTGTGTGCATGATGTAA
3275
PRIMER_PAIR_PENALTY=2.4374
3276
PRIMER_LEFT_PENALTY=0.449829
3277
PRIMER_RIGHT_PENALTY=1.987562
3278
PRIMER_LEFT_SEQUENCE=GATCACTAGGTGCAAAGGGG
3279
PRIMER_RIGHT_SEQUENCE=GCATGTGTGTGCATGATGTAA
3280
PRIMER_LEFT=34,20
3281
PRIMER_RIGHT=207,21
3282
PRIMER_LEFT_TM=59.550
3283
PRIMER_RIGHT_TM=59.012
3284
PRIMER_LEFT_GC_PERCENT=55.000
3285
PRIMER_RIGHT_GC_PERCENT=42.857
3286
PRIMER_LEFT_SELF_ANY=4.00
3287
PRIMER_RIGHT_SELF_ANY=5.00
3288
PRIMER_LEFT_SELF_END=0.00
3289
PRIMER_RIGHT_SELF_END=3.00
3290
PRIMER_LEFT_END_STABILITY=10.9000
3291
PRIMER_RIGHT_END_STABILITY=6.0000
3292
PRIMER_PAIR_COMPL_ANY=4.00
3293
PRIMER_PAIR_COMPL_END=0.00
3294
PRIMER_PRODUCT_SIZE=174
3295
PRIMER_PAIR_PENALTY_1=2.4844
3296
PRIMER_LEFT_1_PENALTY=0.449829
3297
PRIMER_RIGHT_1_PENALTY=2.034537
3298
PRIMER_LEFT_1_SEQUENCE=GATCACTAGGTGCAAAGGGG
3299
PRIMER_RIGHT_1_SEQUENCE=TGTGCATGATGTAAGTGTGGAA
3300
PRIMER_LEFT_1=34,20
3301
PRIMER_RIGHT_1=200,22
3302
PRIMER_LEFT_1_TM=59.550
3303
PRIMER_RIGHT_1_TM=60.035
3304
PRIMER_LEFT_1_GC_PERCENT=55.000
3305
PRIMER_RIGHT_1_GC_PERCENT=40.909
3306
PRIMER_LEFT_1_SELF_ANY=4.00
3307
PRIMER_RIGHT_1_SELF_ANY=5.00
3308
PRIMER_LEFT_1_SELF_END=0.00
3309
PRIMER_RIGHT_1_SELF_END=0.00
3310
PRIMER_LEFT_1_END_STABILITY=10.9000
3311
PRIMER_RIGHT_1_END_STABILITY=8.5000
3312
PRIMER_PAIR_1_COMPL_ANY=4.00
3313
PRIMER_PAIR_1_COMPL_END=0.00
3314
PRIMER_PRODUCT_SIZE_1=167
3315
PRIMER_PAIR_PENALTY_2=2.5150
3316
PRIMER_LEFT_2_PENALTY=0.449829
3317
PRIMER_RIGHT_2_PENALTY=2.065202
3318
PRIMER_LEFT_2_SEQUENCE=GATCACTAGGTGCAAAGGGG
3319
PRIMER_RIGHT_2_SEQUENCE=GCATGTGTGTGCATGATGTAAG
3320
PRIMER_LEFT_2=34,20
3321
PRIMER_RIGHT_2=207,22
3322
PRIMER_LEFT_2_TM=59.550
3323
PRIMER_RIGHT_2_TM=60.065
3324
PRIMER_LEFT_2_GC_PERCENT=55.000
3325
PRIMER_RIGHT_2_GC_PERCENT=45.455
3326
PRIMER_LEFT_2_SELF_ANY=4.00
3327
PRIMER_RIGHT_2_SELF_ANY=5.00
3328
PRIMER_LEFT_2_SELF_END=0.00
3329
PRIMER_RIGHT_2_SELF_END=1.00
3330
PRIMER_LEFT_2_END_STABILITY=10.9000
3331
PRIMER_RIGHT_2_END_STABILITY=5.7000
3332
PRIMER_PAIR_2_COMPL_ANY=4.00
3333
PRIMER_PAIR_2_COMPL_END=0.00
3334
PRIMER_PRODUCT_SIZE_2=174
3335
PRIMER_PAIR_PENALTY_3=2.8922
3336
PRIMER_LEFT_3_PENALTY=0.449829
3337
PRIMER_RIGHT_3_PENALTY=2.442380
3338
PRIMER_LEFT_3_SEQUENCE=GATCACTAGGTGCAAAGGGG
3339
PRIMER_RIGHT_3_SEQUENCE=TGTGCATGATGTAAGTGTGGA
3340
PRIMER_LEFT_3=34,20
3341
PRIMER_RIGHT_3=200,21
3342
PRIMER_LEFT_3_TM=59.550
3343
PRIMER_RIGHT_3_TM=58.558
3344
PRIMER_LEFT_3_GC_PERCENT=55.000
3345
PRIMER_RIGHT_3_GC_PERCENT=42.857
3346
PRIMER_LEFT_3_SELF_ANY=4.00
3347
PRIMER_RIGHT_3_SELF_ANY=5.00
3348
PRIMER_LEFT_3_SELF_END=0.00
3349
PRIMER_RIGHT_3_SELF_END=0.00
3350
PRIMER_LEFT_3_END_STABILITY=10.9000
3351
PRIMER_RIGHT_3_END_STABILITY=7.9000
3352
PRIMER_PAIR_3_COMPL_ANY=4.00
3353
PRIMER_PAIR_3_COMPL_END=0.00
3354
PRIMER_PRODUCT_SIZE_3=167
3355
PRIMER_PAIR_PENALTY_4=2.9442
3356
PRIMER_LEFT_4_PENALTY=0.449829
3357
PRIMER_RIGHT_4_PENALTY=2.494404
3358
PRIMER_LEFT_4_SEQUENCE=GATCACTAGGTGCAAAGGGG
3359
PRIMER_RIGHT_4_SEQUENCE=TGTGTGCATGATGTAAGTGTGG
3360
PRIMER_LEFT_4=34,20
3361
PRIMER_RIGHT_4=202,22
3362
PRIMER_LEFT_4_TM=59.550
3363
PRIMER_RIGHT_4_TM=60.494
3364
PRIMER_LEFT_4_GC_PERCENT=55.000
3365
PRIMER_RIGHT_4_GC_PERCENT=45.455
3366
PRIMER_LEFT_4_SELF_ANY=4.00
3367
PRIMER_RIGHT_4_SELF_ANY=5.00
3368
PRIMER_LEFT_4_SELF_END=0.00
3369
PRIMER_RIGHT_4_SELF_END=0.00
3370
PRIMER_LEFT_4_END_STABILITY=10.9000
3371
PRIMER_RIGHT_4_END_STABILITY=8.2000
3372
PRIMER_PAIR_4_COMPL_ANY=4.00
3373
PRIMER_PAIR_4_COMPL_END=0.00
3374
PRIMER_PRODUCT_SIZE_4=169
3375
=
3376
MARKER_NAME=34.RHAP33FG10.seq
3377
SEQUENCE=NNNNNNNNNNNNNNNNNCTATTTTGATCTCCATATGTTTTATTTCTATATCTATTCCTAATAGTTTTTANNNNNNNNNNNNNNNNGCATGCANNNNNNNNNNNNNNNNNNNNNNNNTCTGCANNNNNNNNNNNNNNNNNNNNNNNNNNTTGCTTAAAGATCAAGAAAGATGAGTTGTACCTGAGAAAACTTGCTAAGTCCCCCACTAGTAGTGGATCGACAATTAGACATTTTCCCATCAAGGACTGAAGCGTCTTTCTTGCGGACAGTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
3378
INCLUDED_REGION=17,254
3379
TARGET=69,16,GT
3380
TARGET=92,24,TG
3381
TARGET=122,26,TG
3382
PRIMER_COMMENT=CL is
3383
PRIMER_COMMENT=CR is
3384
=
3385
MARKER_NAME=35.RHAP33FG11.seq
3386
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNTAGAGACCACTGCGTCTCAGGAAATAAGGTGAAAAGAAACTGAGGAAGACAGCCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCCACTCACACAAGTTTTCTCAATTCCAGGCTATTTCCCCTTAGCGATAAGTGTAAGTTCATTCAAGAGCAGATGCTATTTTTCCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
3387
INCLUDED_REGION=27,174
3388
TARGET=83,30,CA
3389
PRIMER_COMMENT=CL is ACCACTGCGTCTCAGGAAAT
3390
PRIMER_COMMENT=CR is GCTAAGGGGAAATAGCCTGG
3391
PRIMER_PAIR_PENALTY=0.3272
3392
PRIMER_LEFT_PENALTY=0.272667
3393
PRIMER_RIGHT_PENALTY=0.054514
3394
PRIMER_LEFT_SEQUENCE=ACCACTGCGTCTCAGGAAAT
3395
PRIMER_RIGHT_SEQUENCE=GCTAAGGGGAAATAGCCTGG
3396
PRIMER_LEFT=32,20
3397
PRIMER_RIGHT=158,20
3398
PRIMER_LEFT_TM=59.727
3399
PRIMER_RIGHT_TM=60.055
3400
PRIMER_LEFT_GC_PERCENT=50.000
3401
PRIMER_RIGHT_GC_PERCENT=55.000
3402
PRIMER_LEFT_SELF_ANY=5.00
3403
PRIMER_RIGHT_SELF_ANY=4.00
3404
PRIMER_LEFT_SELF_END=2.00
3405
PRIMER_RIGHT_SELF_END=3.00
3406
PRIMER_LEFT_END_STABILITY=6.9000
3407
PRIMER_RIGHT_END_STABILITY=9.7000
3408
PRIMER_PAIR_COMPL_ANY=4.00
3409
PRIMER_PAIR_COMPL_END=3.00
3410
PRIMER_PRODUCT_SIZE=127
3411
PRIMER_PAIR_PENALTY_1=0.4565
3412
PRIMER_LEFT_1_PENALTY=0.272667
3413
PRIMER_RIGHT_1_PENALTY=0.183834
3414
PRIMER_LEFT_1_SEQUENCE=ACCACTGCGTCTCAGGAAAT
3415
PRIMER_RIGHT_1_SEQUENCE=TCGCTAAGGGGAAATAGCCT
3416
PRIMER_LEFT_1=32,20
3417
PRIMER_RIGHT_1=160,20
3418
PRIMER_LEFT_1_TM=59.727
3419
PRIMER_RIGHT_1_TM=60.184
3420
PRIMER_LEFT_1_GC_PERCENT=50.000
3421
PRIMER_RIGHT_1_GC_PERCENT=50.000
3422
PRIMER_LEFT_1_SELF_ANY=5.00
3423
PRIMER_RIGHT_1_SELF_ANY=4.00
3424
PRIMER_LEFT_1_SELF_END=2.00
3425
PRIMER_RIGHT_1_SELF_END=3.00
3426
PRIMER_LEFT_1_END_STABILITY=6.9000
3427
PRIMER_RIGHT_1_END_STABILITY=9.4000
3428
PRIMER_PAIR_1_COMPL_ANY=3.00
3429
PRIMER_PAIR_1_COMPL_END=3.00
3430
PRIMER_PRODUCT_SIZE_1=129
3431
PRIMER_PAIR_PENALTY_2=0.6626
3432
PRIMER_LEFT_2_PENALTY=0.608084
3433
PRIMER_RIGHT_2_PENALTY=0.054514
3434
PRIMER_LEFT_2_SEQUENCE=AGAGACCACTGCGTCTCAGG
3435
PRIMER_RIGHT_2_SEQUENCE=GCTAAGGGGAAATAGCCTGG
3436
PRIMER_LEFT_2=28,20
3437
PRIMER_RIGHT_2=158,20
3438
PRIMER_LEFT_2_TM=60.608
3439
PRIMER_RIGHT_2_TM=60.055
3440
PRIMER_LEFT_2_GC_PERCENT=60.000
3441
PRIMER_RIGHT_2_GC_PERCENT=55.000
3442
PRIMER_LEFT_2_SELF_ANY=9.00
3443
PRIMER_RIGHT_2_SELF_ANY=4.00
3444
PRIMER_LEFT_2_SELF_END=3.00
3445
PRIMER_RIGHT_2_SELF_END=3.00
3446
PRIMER_LEFT_2_END_STABILITY=8.2000
3447
PRIMER_RIGHT_2_END_STABILITY=9.7000
3448
PRIMER_PAIR_2_COMPL_ANY=4.00
3449
PRIMER_PAIR_2_COMPL_END=3.00
3450
PRIMER_PRODUCT_SIZE_2=131
3451
PRIMER_PAIR_PENALTY_3=0.7176
3452
PRIMER_LEFT_3_PENALTY=0.272667
3453
PRIMER_RIGHT_3_PENALTY=0.444960
3454
PRIMER_LEFT_3_SEQUENCE=ACCACTGCGTCTCAGGAAAT
3455
PRIMER_RIGHT_3_SEQUENCE=ATCGCTAAGGGGAAATAGCC
3456
PRIMER_LEFT_3=32,20
3457
PRIMER_RIGHT_3=161,20
3458
PRIMER_LEFT_3_TM=59.727
3459
PRIMER_RIGHT_3_TM=59.555
3460
PRIMER_LEFT_3_GC_PERCENT=50.000
3461
PRIMER_RIGHT_3_GC_PERCENT=50.000
3462
PRIMER_LEFT_3_SELF_ANY=5.00
3463
PRIMER_RIGHT_3_SELF_ANY=4.00
3464
PRIMER_LEFT_3_SELF_END=2.00
3465
PRIMER_RIGHT_3_SELF_END=2.00
3466
PRIMER_LEFT_3_END_STABILITY=6.9000
3467
PRIMER_RIGHT_3_END_STABILITY=8.7000
3468
PRIMER_PAIR_3_COMPL_ANY=3.00
3469
PRIMER_PAIR_3_COMPL_END=2.00
3470
PRIMER_PRODUCT_SIZE_3=130
3471
PRIMER_PAIR_PENALTY_4=0.7919
3472
PRIMER_LEFT_4_PENALTY=0.608084
3473
PRIMER_RIGHT_4_PENALTY=0.183834
3474
PRIMER_LEFT_4_SEQUENCE=AGAGACCACTGCGTCTCAGG
3475
PRIMER_RIGHT_4_SEQUENCE=TCGCTAAGGGGAAATAGCCT
3476
PRIMER_LEFT_4=28,20
3477
PRIMER_RIGHT_4=160,20
3478
PRIMER_LEFT_4_TM=60.608
3479
PRIMER_RIGHT_4_TM=60.184
3480
PRIMER_LEFT_4_GC_PERCENT=60.000
3481
PRIMER_RIGHT_4_GC_PERCENT=50.000
3482
PRIMER_LEFT_4_SELF_ANY=9.00
3483
PRIMER_RIGHT_4_SELF_ANY=4.00
3484
PRIMER_LEFT_4_SELF_END=3.00
3485
PRIMER_RIGHT_4_SELF_END=3.00
3486
PRIMER_LEFT_4_END_STABILITY=8.2000
3487
PRIMER_RIGHT_4_END_STABILITY=9.4000
3488
PRIMER_PAIR_4_COMPL_ANY=3.00
3489
PRIMER_PAIR_4_COMPL_END=3.00
3490
PRIMER_PRODUCT_SIZE_4=133
3491
=
3492
MARKER_NAME=36.RHAP33FG12.seq
3493
SEQUENCE=NNNNNNNNNNNNNNNNNCTTCTGTTTAGATTGTGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATGGTTTCACAGGCTGGCTATTCATGCTTCATGGTCTTCAGTTCTGGGATAACAGATATGGCCACAAGTGGCTTACTTTAAAGTTTTGACATTAACAAACATTTTTGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
3494
INCLUDED_REGION=17,174
3495
TARGET=37,44,GT
3496
PRIMER_COMMENT=CL is
3497
PRIMER_COMMENT=CR is
3498
=
3499
MARKER_NAME=36.RHAP34FG12.seq
3500
SEQUENCE=NNNNNNNNNNNNNNNNNNNCCAGGTTATGCAAGGCTGGTTGGGTGCAGGGCTAAGAGATGCTGAAAAAAAAAGTGTATCTACAATTAGATTATTTCATTTCATTTGCTGTGGAGAATCTACAGGTGTGTGAGTGGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTTTTGAACTACTCTTGGGATAACATGTGGAAGAGTCTGGGTGTGGAAAGCTGTCATTCGGGAAAGAAAAATAAGAGCACTGTGATCCCAGGTACAAACATTTTAGAGGCAGATTTTAGGGNNNN
3501
INCLUDED_REGION=19,276
3502
TARGET=137,36,GT
3503
PRIMER_COMMENT=CL is GCAGGGCTAAGAGATGCTGA
3504
PRIMER_COMMENT=CR is CACACCCAGACTCTTCCACA
3505
PRIMER_PAIR_PENALTY=0.9408
3506
PRIMER_LEFT_PENALTY=0.645726
3507
PRIMER_RIGHT_PENALTY=0.295091
3508
PRIMER_LEFT_SEQUENCE=GCAGGGCTAAGAGATGCTGA
3509
PRIMER_RIGHT_SEQUENCE=CACACCCAGACTCTTCCACA
3510
PRIMER_LEFT=44,20
3511
PRIMER_RIGHT=218,20
3512
PRIMER_LEFT_TM=60.646
3513
PRIMER_RIGHT_TM=59.705
3514
PRIMER_LEFT_GC_PERCENT=55.000
3515
PRIMER_RIGHT_GC_PERCENT=55.000
3516
PRIMER_LEFT_SELF_ANY=3.00
3517
PRIMER_RIGHT_SELF_ANY=5.00
3518
PRIMER_LEFT_SELF_END=1.00
3519
PRIMER_RIGHT_SELF_END=0.00
3520
PRIMER_LEFT_END_STABILITY=8.2000
3521
PRIMER_RIGHT_END_STABILITY=8.2000
3522
PRIMER_PAIR_COMPL_ANY=5.00
3523
PRIMER_PAIR_COMPL_END=2.00
3524
PRIMER_PRODUCT_SIZE=175
3525
PRIMER_PAIR_PENALTY_1=1.2997
3526
PRIMER_LEFT_1_PENALTY=0.396940
3527
PRIMER_RIGHT_1_PENALTY=0.902727
3528
PRIMER_LEFT_1_SEQUENCE=GTGCAGGGCTAAGAGATGCT
3529
PRIMER_RIGHT_1_SEQUENCE=CACCCAGACTCTTCCACATG
3530
PRIMER_LEFT_1=42,20
3531
PRIMER_RIGHT_1=216,20
3532
PRIMER_LEFT_1_TM=59.603
3533
PRIMER_RIGHT_1_TM=59.097
3534
PRIMER_LEFT_1_GC_PERCENT=55.000
3535
PRIMER_RIGHT_1_GC_PERCENT=55.000
3536
PRIMER_LEFT_1_SELF_ANY=4.00
3537
PRIMER_RIGHT_1_SELF_ANY=5.00
3538
PRIMER_LEFT_1_SELF_END=2.00
3539
PRIMER_RIGHT_1_SELF_END=4.00
3540
PRIMER_LEFT_1_END_STABILITY=8.1000
3541
PRIMER_RIGHT_1_END_STABILITY=6.6000
3542
PRIMER_PAIR_1_COMPL_ANY=5.00
3543
PRIMER_PAIR_1_COMPL_END=3.00
3544
PRIMER_PRODUCT_SIZE_1=175
3545
PRIMER_PAIR_PENALTY_2=1.4145
3546
PRIMER_LEFT_2_PENALTY=0.396940
3547
PRIMER_RIGHT_2_PENALTY=1.017601
3548
PRIMER_LEFT_2_SEQUENCE=GTGCAGGGCTAAGAGATGCT
3549
PRIMER_RIGHT_2_SEQUENCE=CACCCAGACTCTTCCACATGT
3550
PRIMER_LEFT_2=42,20
3551
PRIMER_RIGHT_2=216,21
3552
PRIMER_LEFT_2_TM=59.603
3553
PRIMER_RIGHT_2_TM=60.018
3554
PRIMER_LEFT_2_GC_PERCENT=55.000
3555
PRIMER_RIGHT_2_GC_PERCENT=52.381
3556
PRIMER_LEFT_2_SELF_ANY=4.00
3557
PRIMER_RIGHT_2_SELF_ANY=6.00
3558
PRIMER_LEFT_2_SELF_END=2.00
3559
PRIMER_RIGHT_2_SELF_END=6.00
3560
PRIMER_LEFT_2_END_STABILITY=8.1000
3561
PRIMER_RIGHT_2_END_STABILITY=6.6000
3562
PRIMER_PAIR_2_COMPL_ANY=5.00
3563
PRIMER_PAIR_2_COMPL_END=2.00
3564
PRIMER_PRODUCT_SIZE_2=175
3565
PRIMER_PAIR_PENALTY_3=1.5485
3566
PRIMER_LEFT_3_PENALTY=0.645726
3567
PRIMER_RIGHT_3_PENALTY=0.902727
3568
PRIMER_LEFT_3_SEQUENCE=GCAGGGCTAAGAGATGCTGA
3569
PRIMER_RIGHT_3_SEQUENCE=CACCCAGACTCTTCCACATG
3570
PRIMER_LEFT_3=44,20
3571
PRIMER_RIGHT_3=216,20
3572
PRIMER_LEFT_3_TM=60.646
3573
PRIMER_RIGHT_3_TM=59.097
3574
PRIMER_LEFT_3_GC_PERCENT=55.000
3575
PRIMER_RIGHT_3_GC_PERCENT=55.000
3576
PRIMER_LEFT_3_SELF_ANY=3.00
3577
PRIMER_RIGHT_3_SELF_ANY=5.00
3578
PRIMER_LEFT_3_SELF_END=1.00
3579
PRIMER_RIGHT_3_SELF_END=4.00
3580
PRIMER_LEFT_3_END_STABILITY=8.2000
3581
PRIMER_RIGHT_3_END_STABILITY=6.6000
3582
PRIMER_PAIR_3_COMPL_ANY=5.00
3583
PRIMER_PAIR_3_COMPL_END=3.00
3584
PRIMER_PRODUCT_SIZE_3=173
3585
PRIMER_PAIR_PENALTY_4=1.6633
3586
PRIMER_LEFT_4_PENALTY=0.645726
3587
PRIMER_RIGHT_4_PENALTY=1.017601
3588
PRIMER_LEFT_4_SEQUENCE=GCAGGGCTAAGAGATGCTGA
3589
PRIMER_RIGHT_4_SEQUENCE=CACACCCAGACTCTTCCACAT
3590
PRIMER_LEFT_4=44,20
3591
PRIMER_RIGHT_4=218,21
3592
PRIMER_LEFT_4_TM=60.646
3593
PRIMER_RIGHT_4_TM=60.018
3594
PRIMER_LEFT_4_GC_PERCENT=55.000
3595
PRIMER_RIGHT_4_GC_PERCENT=52.381
3596
PRIMER_LEFT_4_SELF_ANY=3.00
3597
PRIMER_RIGHT_4_SELF_ANY=5.00
3598
PRIMER_LEFT_4_SELF_END=1.00
3599
PRIMER_RIGHT_4_SELF_END=2.00
3600
PRIMER_LEFT_4_END_STABILITY=8.2000
3601
PRIMER_RIGHT_4_END_STABILITY=6.6000
3602
PRIMER_PAIR_4_COMPL_ANY=5.00
3603
PRIMER_PAIR_4_COMPL_END=3.00
3604
PRIMER_PRODUCT_SIZE_4=175
3605
=
3606
MARKER_NAME=37.RHAP33FD1.seq
3607
SEQUENCE=NNNNNNNNNNCTTCACTTTCATACATGGAAGTCACTATCTCAGAGCAGATACCCTGGTCCTCTGTCCCTTACAATCTCTCCGTGCTATCTTTCAGAGATGTTCCCTAAGCCTTAGGTGTAGGGGTTGTGTTGAAGATGCACCAAGGATAAGGATTCAGTCAGTTGTCTGCGTTTTTTTTTACCAGTTCTGAGTTTCTCTGATACCCTTTATCTATATGCAGCAGCTGGCAGCAGTATGTACTGTTGGCAAACTGATAAAATGTTCTGGATCTGTAATGAAAGACACACATATAGATAAGTATATANACATNNNNNNNNNNNNNNNNAAGNGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGNCA
3608
INCLUDED_REGION=10,361
3609
TARGET=310,16,AC
3610
TARGET=333,33,CA
3611
PRIMER_COMMENT=CL is
3612
PRIMER_COMMENT=CR is
3613
=
3614
MARKER_NAME=37.RHAP33FH1.seq
3615
SEQUENCE=NNNNTCCCGGTACGGGCGCGCCCNCCCCCGNNNNNNNNNNNNNNNNNNNNNTGTGGNGCGCGTTTGTCGGNTTTGNGTCCAGAGGCTGTTGTTAGGTATGGAGGGNGTGGAGGNGGAGGGTGTTCTTGGTGCCAGTGTGTGTNCGCNGCTGTTGGCGTTGNNNTTTGNGGGNGTNTGANGACCAANTGGCCNACGGTGCTGTGACATCAACCAGCTGGCAAAGNAAGNAGGNGCNAAGAGCGCAGCCTTCCCANGCCNNCACNGCNNNACCTCCTGCNNNTCTCCTAACCCCNNNCNCCCNCTTCNGCCTNCNCCTGNNCCTCCCTCAAGGCCGCCNNCCCNNTGTCCNNCNNCCCGCCCNNCNGGAANAGCCCCTCCACCCCCNNCNGCCNCACCNNCAATCCTCCNCNTNAACCCNCTGCCNCCT
3616
INCLUDED_REGION=4,423
3617
TARGET=30,21,GT
3618
PRIMER_COMMENT=CL is
3619
PRIMER_COMMENT=CR is
3620
=
3621
MARKER_NAME=37.RHAP34FD1.seq
3622
SEQUENCE=NNNNNNNNNNNNNNNNNNACTACAAGGNNNNNNNNNNNNNNNNNNNATTCATATATACAGCACACGCATACAAATNNNNNNNNNNNNNNNNNNNNAACAAACATACTTACTTACACACCACACAGACCCACTACGAACTCACACAAACATTCTACATAGACACATCACATACATACCATGCAGTNNNNNNNNNNNNNNNNNNCTCATACCACACCCAATACACACACACACGTCTACTCTCTACTTCGTCNACTATCGACNACTACACACACNACNNCATCTATCTACNGCATTTACTTTACATACAAAGTTATACAGTAGNAGTAGAGTGNACCGATGACTCNGA
3623
INCLUDED_REGION=18,328
3624
TARGET=27,19,AC
3625
TARGET=75,20,CA
3626
TARGET=184,18,CA
3627
PRIMER_COMMENT=CL is ACTTACACACCACACAGACCC
3628
PRIMER_COMMENT=CR is GACGTGTGTGTGTGTATTGGG
3629
PRIMER_PAIR_PENALTY=4.8203
3630
PRIMER_LEFT_PENALTY=3.605542
3631
PRIMER_RIGHT_PENALTY=1.214710
3632
PRIMER_LEFT_SEQUENCE=ACTTACACACCACACAGACCC
3633
PRIMER_RIGHT_SEQUENCE=GACGTGTGTGTGTGTATTGGG
3634
PRIMER_LEFT=108,21
3635
PRIMER_RIGHT=233,21
3636
PRIMER_LEFT_TM=57.394
3637
PRIMER_RIGHT_TM=59.785
3638
PRIMER_LEFT_GC_PERCENT=52.381
3639
PRIMER_RIGHT_GC_PERCENT=52.381
3640
PRIMER_LEFT_SELF_ANY=2.00
3641
PRIMER_RIGHT_SELF_ANY=4.00
3642
PRIMER_LEFT_SELF_END=0.00
3643
PRIMER_RIGHT_SELF_END=0.00
3644
PRIMER_LEFT_END_STABILITY=9.1000
3645
PRIMER_RIGHT_END_STABILITY=10.0000
3646
PRIMER_PAIR_COMPL_ANY=10.00
3647
PRIMER_PAIR_COMPL_END=6.00
3648
PRIMER_PRODUCT_SIZE=126
3649
PRIMER_PAIR_PENALTY_1=5.2679
3650
PRIMER_LEFT_1_PENALTY=4.053183
3651
PRIMER_RIGHT_1_PENALTY=1.214710
3652
PRIMER_LEFT_1_SEQUENCE=TTACTTACACACCACACAGACCC
3653
PRIMER_RIGHT_1_SEQUENCE=GACGTGTGTGTGTGTATTGGG
3654
PRIMER_LEFT_1=106,23
3655
PRIMER_RIGHT_1=233,21
3656
PRIMER_LEFT_1_TM=58.947
3657
PRIMER_RIGHT_1_TM=59.785
3658
PRIMER_LEFT_1_GC_PERCENT=47.826
3659
PRIMER_RIGHT_1_GC_PERCENT=52.381
3660
PRIMER_LEFT_1_SELF_ANY=2.00
3661
PRIMER_RIGHT_1_SELF_ANY=4.00
3662
PRIMER_LEFT_1_SELF_END=0.00
3663
PRIMER_RIGHT_1_SELF_END=0.00
3664
PRIMER_LEFT_1_END_STABILITY=9.1000
3665
PRIMER_RIGHT_1_END_STABILITY=10.0000
3666
PRIMER_PAIR_1_COMPL_ANY=10.00
3667
PRIMER_PAIR_1_COMPL_END=6.00
3668
PRIMER_PRODUCT_SIZE_1=128
3669
PRIMER_PAIR_PENALTY_2=5.3251
3670
PRIMER_LEFT_2_PENALTY=4.110393
3671
PRIMER_RIGHT_2_PENALTY=1.214710
3672
PRIMER_LEFT_2_SEQUENCE=CTTACTTACACACCACACAGACCC
3673
PRIMER_RIGHT_2_SEQUENCE=GACGTGTGTGTGTGTATTGGG
3674
PRIMER_LEFT_2=105,24
3675
PRIMER_RIGHT_2=233,21
3676
PRIMER_LEFT_2_TM=59.890
3677
PRIMER_RIGHT_2_TM=59.785
3678
PRIMER_LEFT_2_GC_PERCENT=50.000
3679
PRIMER_RIGHT_2_GC_PERCENT=52.381
3680
PRIMER_LEFT_2_SELF_ANY=2.00
3681
PRIMER_RIGHT_2_SELF_ANY=4.00
3682
PRIMER_LEFT_2_SELF_END=0.00
3683
PRIMER_RIGHT_2_SELF_END=0.00
3684
PRIMER_LEFT_2_END_STABILITY=9.1000
3685
PRIMER_RIGHT_2_END_STABILITY=10.0000
3686
PRIMER_PAIR_2_COMPL_ANY=10.00
3687
PRIMER_PAIR_2_COMPL_END=6.00
3688
PRIMER_PRODUCT_SIZE_2=129
3689
PRIMER_PAIR_PENALTY_3=5.7055
3690
PRIMER_LEFT_3_PENALTY=4.490770
3691
PRIMER_RIGHT_3_PENALTY=1.214710
3692
PRIMER_LEFT_3_SEQUENCE=TACTTACACACCACACAGACCC
3693
PRIMER_RIGHT_3_SEQUENCE=GACGTGTGTGTGTGTATTGGG
3694
PRIMER_LEFT_3=107,22
3695
PRIMER_RIGHT_3=233,21
3696
PRIMER_LEFT_3_TM=57.509
3697
PRIMER_RIGHT_3_TM=59.785
3698
PRIMER_LEFT_3_GC_PERCENT=50.000
3699
PRIMER_RIGHT_3_GC_PERCENT=52.381
3700
PRIMER_LEFT_3_SELF_ANY=2.00
3701
PRIMER_RIGHT_3_SELF_ANY=4.00
3702
PRIMER_LEFT_3_SELF_END=0.00
3703
PRIMER_RIGHT_3_SELF_END=0.00
3704
PRIMER_LEFT_3_END_STABILITY=9.1000
3705
PRIMER_RIGHT_3_END_STABILITY=10.0000
3706
PRIMER_PAIR_3_COMPL_ANY=10.00
3707
PRIMER_PAIR_3_COMPL_END=6.00
3708
PRIMER_PRODUCT_SIZE_3=127
3709
PRIMER_PAIR_PENALTY_4=5.7739
3710
PRIMER_LEFT_4_PENALTY=0.063544
3711
PRIMER_RIGHT_4_PENALTY=5.710325
3712
PRIMER_LEFT_4_SEQUENCE=CACCACACAGACCCACTACG
3713
PRIMER_RIGHT_4_SEQUENCE=CGAAGTAGAGAGTAGACGTGTGTG
3714
PRIMER_LEFT_4=115,20
3715
PRIMER_RIGHT_4=247,24
3716
PRIMER_LEFT_4_TM=60.064
3717
PRIMER_RIGHT_4_TM=58.290
3718
PRIMER_LEFT_4_GC_PERCENT=60.000
3719
PRIMER_RIGHT_4_GC_PERCENT=50.000
3720
PRIMER_LEFT_4_SELF_ANY=2.00
3721
PRIMER_RIGHT_4_SELF_ANY=4.00
3722
PRIMER_LEFT_4_SELF_END=2.00
3723
PRIMER_RIGHT_4_SELF_END=0.00
3724
PRIMER_LEFT_4_END_STABILITY=7.4000
3725
PRIMER_RIGHT_4_END_STABILITY=6.4000
3726
PRIMER_PAIR_4_COMPL_ANY=6.00
3727
PRIMER_PAIR_4_COMPL_END=4.00
3728
PRIMER_PRODUCT_SIZE_4=133
3729
=
3730
MARKER_NAME=37.RHAP34FH1.seq
3731
SEQUENCE=NNNNNNNNNNNNNNNNNNCCTGCATCTCCATACTCCATTGATGTAGTGCTAGGGACAGAATTCAGGGCATCATGCATGCTACAAACTAAGCTACATCCCTAGCCCCACATGTGAAGTCTTTTGTAACTGATGTCTACTGATTTGAACCTAAGGAGGCTATAAGAGAAGAGAACCACTGAACTCCATGAGATTTAGAACAGGAAATGGAATGGAGACTATTATTTTTTCCACTGTTTCTCTGTTTCTCTGTGTTTCTCTTANNNNNNNNNNNNNNNNNNNNTCTGTCCTGTTTAAGTGTGGA
3732
INCLUDED_REGION=18,283
3733
TARGET=260,20,TG
3734
PRIMER_COMMENT=CL is
3735
PRIMER_COMMENT=CR is
3736
=
3737
MARKER_NAME=38.RHAP34FH2.seq
3738
SEQUENCE=NNNNNNNNNNNNNNNNNNNNCCTGGGTTCGGTCCCCAGCTCCGGGGGGAAAAAAAAAAAAGAATTGCAGGTCAGTGTCATACAGGTCTTGGTGGTCTGTTTTCTTTAGTCTGTTTTATTCCATGCTGTCACACAGTAAAGATGCAGTCTACTACTGAGTTACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGTATCCACATGCCATAGAATGTGTGTTGGAGGTCTGAAGAATAGCTTTCAGAGAGTTGGTTCATCATCTTTCATCATATTTTTTTTCCCCAGAAATTG
3739
INCLUDED_REGION=20,282
3740
TARGET=162,40,GT
3741
PRIMER_COMMENT=CL is TGCAGGTCAGTGTCATACAGG
3742
PRIMER_COMMENT=CR is TCCAACACACATTCTATGGCA
3743
PRIMER_PAIR_PENALTY=2.2597
3744
PRIMER_LEFT_PENALTY=1.239519
3745
PRIMER_RIGHT_PENALTY=1.020151
3746
PRIMER_LEFT_SEQUENCE=TGCAGGTCAGTGTCATACAGG
3747
PRIMER_RIGHT_SEQUENCE=TCCAACACACATTCTATGGCA
3748
PRIMER_LEFT=64,21
3749
PRIMER_RIGHT=233,21
3750
PRIMER_LEFT_TM=59.760
3751
PRIMER_RIGHT_TM=59.980
3752
PRIMER_LEFT_GC_PERCENT=52.381
3753
PRIMER_RIGHT_GC_PERCENT=42.857
3754
PRIMER_LEFT_SELF_ANY=5.00
3755
PRIMER_RIGHT_SELF_ANY=3.00
3756
PRIMER_LEFT_SELF_END=1.00
3757
PRIMER_RIGHT_SELF_END=3.00
3758
PRIMER_LEFT_END_STABILITY=7.9000
3759
PRIMER_RIGHT_END_STABILITY=10.0000
3760
PRIMER_PAIR_COMPL_ANY=5.00
3761
PRIMER_PAIR_COMPL_END=3.00
3762
PRIMER_PRODUCT_SIZE=170
3763
PRIMER_PAIR_PENALTY_1=2.9227
3764
PRIMER_LEFT_1_PENALTY=1.902566
3765
PRIMER_RIGHT_1_PENALTY=1.020151
3766
PRIMER_LEFT_1_SEQUENCE=CAGGTCTTGGTGGTCTGTTTT
3767
PRIMER_RIGHT_1_SEQUENCE=TCCAACACACATTCTATGGCA
3768
PRIMER_LEFT_1=81,21
3769
PRIMER_RIGHT_1=233,21
3770
PRIMER_LEFT_1_TM=59.097
3771
PRIMER_RIGHT_1_TM=59.980
3772
PRIMER_LEFT_1_GC_PERCENT=47.619
3773
PRIMER_RIGHT_1_GC_PERCENT=42.857
3774
PRIMER_LEFT_1_SELF_ANY=3.00
3775
PRIMER_RIGHT_1_SELF_ANY=3.00
3776
PRIMER_LEFT_1_SELF_END=0.00
3777
PRIMER_RIGHT_1_SELF_END=3.00
3778
PRIMER_LEFT_1_END_STABILITY=7.0000
3779
PRIMER_RIGHT_1_END_STABILITY=10.0000
3780
PRIMER_PAIR_1_COMPL_ANY=5.00
3781
PRIMER_PAIR_1_COMPL_END=1.00
3782
PRIMER_PRODUCT_SIZE_1=153
3783
PRIMER_PAIR_PENALTY_2=3.0482
3784
PRIMER_LEFT_2_PENALTY=2.028056
3785
PRIMER_RIGHT_2_PENALTY=1.020151
3786
PRIMER_LEFT_2_SEQUENCE=TCATACAGGTCTTGGTGGTCTG
3787
PRIMER_RIGHT_2_SEQUENCE=TCCAACACACATTCTATGGCA
3788
PRIMER_LEFT_2=76,22
3789
PRIMER_RIGHT_2=233,21
3790
PRIMER_LEFT_2_TM=60.028
3791
PRIMER_RIGHT_2_TM=59.980
3792
PRIMER_LEFT_2_GC_PERCENT=50.000
3793
PRIMER_RIGHT_2_GC_PERCENT=42.857
3794
PRIMER_LEFT_2_SELF_ANY=3.00
3795
PRIMER_RIGHT_2_SELF_ANY=3.00
3796
PRIMER_LEFT_2_SELF_END=1.00
3797
PRIMER_RIGHT_2_SELF_END=3.00
3798
PRIMER_LEFT_2_END_STABILITY=6.4000
3799
PRIMER_RIGHT_2_END_STABILITY=10.0000
3800
PRIMER_PAIR_2_COMPL_ANY=5.00
3801
PRIMER_PAIR_2_COMPL_END=2.00
3802
PRIMER_PRODUCT_SIZE_2=158
3803
PRIMER_PAIR_PENALTY_3=3.0821
3804
PRIMER_LEFT_3_PENALTY=2.061961
3805
PRIMER_RIGHT_3_PENALTY=1.020151
3806
PRIMER_LEFT_3_SEQUENCE=ACAGGTCTTGGTGGTCTGTTTT
3807
PRIMER_RIGHT_3_SEQUENCE=TCCAACACACATTCTATGGCA
3808
PRIMER_LEFT_3=80,22
3809
PRIMER_RIGHT_3=233,21
3810
PRIMER_LEFT_3_TM=59.938
3811
PRIMER_RIGHT_3_TM=59.980
3812
PRIMER_LEFT_3_GC_PERCENT=45.455
3813
PRIMER_RIGHT_3_GC_PERCENT=42.857
3814
PRIMER_LEFT_3_SELF_ANY=4.00
3815
PRIMER_RIGHT_3_SELF_ANY=3.00
3816
PRIMER_LEFT_3_SELF_END=0.00
3817
PRIMER_RIGHT_3_SELF_END=3.00
3818
PRIMER_LEFT_3_END_STABILITY=7.0000
3819
PRIMER_RIGHT_3_END_STABILITY=10.0000
3820
PRIMER_PAIR_3_COMPL_ANY=5.00
3821
PRIMER_PAIR_3_COMPL_END=1.00
3822
PRIMER_PRODUCT_SIZE_3=154
3823
PRIMER_PAIR_PENALTY_4=3.1294
3824
PRIMER_LEFT_4_PENALTY=2.109200
3825
PRIMER_RIGHT_4_PENALTY=1.020151
3826
PRIMER_LEFT_4_SEQUENCE=TGTCATACAGGTCTTGGTGGTC
3827
PRIMER_RIGHT_4_SEQUENCE=TCCAACACACATTCTATGGCA
3828
PRIMER_LEFT_4=74,22
3829
PRIMER_RIGHT_4=233,21
3830
PRIMER_LEFT_4_TM=59.891
3831
PRIMER_RIGHT_4_TM=59.980
3832
PRIMER_LEFT_4_GC_PERCENT=50.000
3833
PRIMER_RIGHT_4_GC_PERCENT=42.857
3834
PRIMER_LEFT_4_SELF_ANY=4.00
3835
PRIMER_RIGHT_4_SELF_ANY=3.00
3836
PRIMER_LEFT_4_SELF_END=1.00
3837
PRIMER_RIGHT_4_SELF_END=3.00
3838
PRIMER_LEFT_4_END_STABILITY=7.9000
3839
PRIMER_RIGHT_4_END_STABILITY=10.0000
3840
PRIMER_PAIR_4_COMPL_ANY=5.00
3841
PRIMER_PAIR_4_COMPL_END=4.00
3842
PRIMER_PRODUCT_SIZE_4=160
3843
=
3844
MARKER_NAME=39.RHAP33FD3.seq
3845
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCTTTTTGGAACACAAGTGNNNNNNNNNNNNNNNNNNNNAATCCTTTTAAGCCTTTTTAAAGAAATGATTGACATTGTGAATTAGACAATTTTCAGTTATAAATTTTCAGCACACAGCACAGAAAGCCTAAATGTGCAATAAAATGCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
3846
INCLUDED_REGION=21,148
3847
TARGET=39,20,CA
3848
PRIMER_COMMENT=CL is
3849
PRIMER_COMMENT=CR is
3850
=
3851
MARKER_NAME=39.RHAP33FH3.seq
3852
SEQUENCE=NNNNNNNNNNNNNNTATTTCTCTGTCTTTTCCTTCTACGTGCTTTCTTTCTTTTTCCCATCTCCCTAAACTCAACCAGGATTTACAGGAGAGAGGGACTTTACCATCTNNNNNNNNNNNNNNNNNNNNCCCACTGCACTCAAGCGCTCGCACGGTGCCCCTCCTCACAGCCTGTGTGCTTTGCTCTCTTCATATATGATTGACAGGGAGAAAAAAAATGAGAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
3853
INCLUDED_REGION=14,211
3854
TARGET=108,20,CA
3855
PRIMER_COMMENT=CL is CCCATCTCCCTAAACTCAACC
3856
PRIMER_COMMENT=CR is GAGAGCAAAGCACACAGGCT
3857
PRIMER_PAIR_PENALTY=1.9302
3858
PRIMER_LEFT_PENALTY=1.187326
3859
PRIMER_RIGHT_PENALTY=0.742877
3860
PRIMER_LEFT_SEQUENCE=CCCATCTCCCTAAACTCAACC
3861
PRIMER_RIGHT_SEQUENCE=AGAGAGCAAAGCACACAGGC
3862
PRIMER_LEFT=55,21
3863
PRIMER_RIGHT=187,20
3864
PRIMER_LEFT_TM=59.813
3865
PRIMER_RIGHT_TM=60.743
3866
PRIMER_LEFT_GC_PERCENT=52.381
3867
PRIMER_RIGHT_GC_PERCENT=55.000
3868
PRIMER_LEFT_SELF_ANY=2.00
3869
PRIMER_RIGHT_SELF_ANY=2.00
3870
PRIMER_LEFT_SELF_END=0.00
3871
PRIMER_RIGHT_SELF_END=2.00
3872
PRIMER_LEFT_END_STABILITY=8.2000
3873
PRIMER_RIGHT_END_STABILITY=9.7000
3874
PRIMER_PAIR_COMPL_ANY=4.00
3875
PRIMER_PAIR_COMPL_END=1.00
3876
PRIMER_PRODUCT_SIZE=133
3877
PRIMER_PAIR_PENALTY_1=1.9302
3878
PRIMER_LEFT_1_PENALTY=1.187326
3879
PRIMER_RIGHT_1_PENALTY=0.742877
3880
PRIMER_LEFT_1_SEQUENCE=CCCATCTCCCTAAACTCAACC
3881
PRIMER_RIGHT_1_SEQUENCE=GAGAGCAAAGCACACAGGCT
3882
PRIMER_LEFT_1=55,21
3883
PRIMER_RIGHT_1=186,20
3884
PRIMER_LEFT_1_TM=59.813
3885
PRIMER_RIGHT_1_TM=60.743
3886
PRIMER_LEFT_1_GC_PERCENT=52.381
3887
PRIMER_RIGHT_1_GC_PERCENT=55.000
3888
PRIMER_LEFT_1_SELF_ANY=2.00
3889
PRIMER_RIGHT_1_SELF_ANY=3.00
3890
PRIMER_LEFT_1_SELF_END=0.00
3891
PRIMER_RIGHT_1_SELF_END=3.00
3892
PRIMER_LEFT_1_END_STABILITY=8.2000
3893
PRIMER_RIGHT_1_END_STABILITY=9.4000
3894
PRIMER_PAIR_1_COMPL_ANY=4.00
3895
PRIMER_PAIR_1_COMPL_END=2.00
3896
PRIMER_PRODUCT_SIZE_1=132
3897
PRIMER_PAIR_PENALTY_2=1.9660
3898
PRIMER_LEFT_2_PENALTY=1.223158
3899
PRIMER_RIGHT_2_PENALTY=0.742877
3900
PRIMER_LEFT_2_SEQUENCE=CGTGCTTTCTTTCTTTTTCCC
3901
PRIMER_RIGHT_2_SEQUENCE=GAGAGCAAAGCACACAGGCT
3902
PRIMER_LEFT_2=37,21
3903
PRIMER_RIGHT_2=186,20
3904
PRIMER_LEFT_2_TM=60.223
3905
PRIMER_RIGHT_2_TM=60.743
3906
PRIMER_LEFT_2_GC_PERCENT=42.857
3907
PRIMER_RIGHT_2_GC_PERCENT=55.000
3908
PRIMER_LEFT_2_SELF_ANY=2.00
3909
PRIMER_RIGHT_2_SELF_ANY=3.00
3910
PRIMER_LEFT_2_SELF_END=0.00
3911
PRIMER_RIGHT_2_SELF_END=3.00
3912
PRIMER_LEFT_2_END_STABILITY=9.7000
3913
PRIMER_RIGHT_2_END_STABILITY=9.4000
3914
PRIMER_PAIR_2_COMPL_ANY=8.00
3915
PRIMER_PAIR_2_COMPL_END=1.00
3916
PRIMER_PRODUCT_SIZE_2=150
3917
PRIMER_PAIR_PENALTY_3=1.9660
3918
PRIMER_LEFT_3_PENALTY=1.223158
3919
PRIMER_RIGHT_3_PENALTY=0.742877
3920
PRIMER_LEFT_3_SEQUENCE=CGTGCTTTCTTTCTTTTTCCC
3921
PRIMER_RIGHT_3_SEQUENCE=AGAGAGCAAAGCACACAGGC
3922
PRIMER_LEFT_3=37,21
3923
PRIMER_RIGHT_3=187,20
3924
PRIMER_LEFT_3_TM=60.223
3925
PRIMER_RIGHT_3_TM=60.743
3926
PRIMER_LEFT_3_GC_PERCENT=42.857
3927
PRIMER_RIGHT_3_GC_PERCENT=55.000
3928
PRIMER_LEFT_3_SELF_ANY=2.00
3929
PRIMER_RIGHT_3_SELF_ANY=2.00
3930
PRIMER_LEFT_3_SELF_END=0.00
3931
PRIMER_RIGHT_3_SELF_END=2.00
3932
PRIMER_LEFT_3_END_STABILITY=9.7000
3933
PRIMER_RIGHT_3_END_STABILITY=9.7000
3934
PRIMER_PAIR_3_COMPL_ANY=9.00
3935
PRIMER_PAIR_3_COMPL_END=2.00
3936
PRIMER_PRODUCT_SIZE_3=151
3937
PRIMER_PAIR_PENALTY_4=2.2243
3938
PRIMER_LEFT_4_PENALTY=1.481423
3939
PRIMER_RIGHT_4_PENALTY=0.742877
3940
PRIMER_LEFT_4_SEQUENCE=TCTTTCTTTTTCCCATCTCCC
3941
PRIMER_RIGHT_4_SEQUENCE=AGAGAGCAAAGCACACAGGC
3942
PRIMER_LEFT_4=44,21
3943
PRIMER_RIGHT_4=187,20
3944
PRIMER_LEFT_4_TM=59.519
3945
PRIMER_RIGHT_4_TM=60.743
3946
PRIMER_LEFT_4_GC_PERCENT=42.857
3947
PRIMER_RIGHT_4_GC_PERCENT=55.000
3948
PRIMER_LEFT_4_SELF_ANY=2.00
3949
PRIMER_RIGHT_4_SELF_ANY=2.00
3950
PRIMER_LEFT_4_SELF_END=0.00
3951
PRIMER_RIGHT_4_SELF_END=2.00
3952
PRIMER_LEFT_4_END_STABILITY=9.4000
3953
PRIMER_RIGHT_4_END_STABILITY=9.7000
3954
PRIMER_PAIR_4_COMPL_ANY=4.00
3955
PRIMER_PAIR_4_COMPL_END=1.00
3956
PRIMER_PRODUCT_SIZE_4=144
3957
=
3958
MARKER_NAME=39.RHAP34FH3.seq
3959
SEQUENCE=NNNNNNNNNNNNNNNNNNCCTGTATGGAATAAAGTTTAAACATTTTTAAGTGTAACATAATAGTAAGCATACGACTGAAATGAATGTGATAGAGAAGGAAGTGGTTTGCCCAGCAAGGAATGTCATGACATCATAAAGTTATTCTGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTGATTATATACATGCATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTCTGCTTCTTTTATTTCACATACAAAGGCCGTACTCTTGGAACTGTGAGTCAAAATAAGGACTTTGNCCNTG
3960
INCLUDED_REGION=18,286
3961
TARGET=148,32,TA
3962
TARGET=202,28,AC
3963
PRIMER_COMMENT=CL is CCCAGCAAGGAATGTCATG
3964
PRIMER_COMMENT=CR is TTCCAAGAGTACGGCCTTTG
3965
PRIMER_PAIR_PENALTY=1.2968
3966
PRIMER_LEFT_PENALTY=1.055809
3967
PRIMER_RIGHT_PENALTY=0.241032
3968
PRIMER_LEFT_SEQUENCE=CCCAGCAAGGAATGTCATG
3969
PRIMER_RIGHT_SEQUENCE=TTCCAAGAGTACGGCCTTTG
3970
PRIMER_LEFT=108,19
3971
PRIMER_RIGHT=273,20
3972
PRIMER_LEFT_TM=60.056
3973
PRIMER_RIGHT_TM=60.241
3974
PRIMER_LEFT_GC_PERCENT=52.632
3975
PRIMER_RIGHT_GC_PERCENT=50.000
3976
PRIMER_LEFT_SELF_ANY=5.00
3977
PRIMER_RIGHT_SELF_ANY=4.00
3978
PRIMER_LEFT_SELF_END=4.00
3979
PRIMER_RIGHT_SELF_END=4.00
3980
PRIMER_LEFT_END_STABILITY=6.9000
3981
PRIMER_RIGHT_END_STABILITY=7.3000
3982
PRIMER_PAIR_COMPL_ANY=4.00
3983
PRIMER_PAIR_COMPL_END=0.00
3984
PRIMER_PRODUCT_SIZE=166
3985
PRIMER_PAIR_PENALTY_1=1.3215
3986
PRIMER_LEFT_1_PENALTY=1.055809
3987
PRIMER_RIGHT_1_PENALTY=0.265715
3988
PRIMER_LEFT_1_SEQUENCE=CCCAGCAAGGAATGTCATG
3989
PRIMER_RIGHT_1_SEQUENCE=TCCAAGAGTACGGCCTTTGT
3990
PRIMER_LEFT_1=108,19
3991
PRIMER_RIGHT_1=272,20
3992
PRIMER_LEFT_1_TM=60.056
3993
PRIMER_RIGHT_1_TM=59.734
3994
PRIMER_LEFT_1_GC_PERCENT=52.632
3995
PRIMER_RIGHT_1_GC_PERCENT=50.000
3996
PRIMER_LEFT_1_SELF_ANY=5.00
3997
PRIMER_RIGHT_1_SELF_ANY=4.00
3998
PRIMER_LEFT_1_SELF_END=4.00
3999
PRIMER_RIGHT_1_SELF_END=2.00
4000
PRIMER_LEFT_1_END_STABILITY=6.9000
4001
PRIMER_RIGHT_1_END_STABILITY=7.0000
4002
PRIMER_PAIR_1_COMPL_ANY=4.00
4003
PRIMER_PAIR_1_COMPL_END=0.00
4004
PRIMER_PRODUCT_SIZE_1=165
4005
PRIMER_PAIR_PENALTY_2=1.7032
4006
PRIMER_LEFT_2_PENALTY=1.055809
4007
PRIMER_RIGHT_2_PENALTY=0.647368
4008
PRIMER_LEFT_2_SEQUENCE=CCCAGCAAGGAATGTCATG
4009
PRIMER_RIGHT_2_SEQUENCE=CAGTTCCAAGAGTACGGCCT
4010
PRIMER_LEFT_2=108,19
4011
PRIMER_RIGHT_2=276,20
4012
PRIMER_LEFT_2_TM=60.056
4013
PRIMER_RIGHT_2_TM=59.353
4014
PRIMER_LEFT_2_GC_PERCENT=52.632
4015
PRIMER_RIGHT_2_GC_PERCENT=55.000
4016
PRIMER_LEFT_2_SELF_ANY=5.00
4017
PRIMER_RIGHT_2_SELF_ANY=4.00
4018
PRIMER_LEFT_2_SELF_END=4.00
4019
PRIMER_RIGHT_2_SELF_END=2.00
4020
PRIMER_LEFT_2_END_STABILITY=6.9000
4021
PRIMER_RIGHT_2_END_STABILITY=10.9000
4022
PRIMER_PAIR_2_COMPL_ANY=4.00
4023
PRIMER_PAIR_2_COMPL_END=1.00
4024
PRIMER_PRODUCT_SIZE_2=169
4025
PRIMER_PAIR_PENALTY_3=1.8440
4026
PRIMER_LEFT_3_PENALTY=1.055809
4027
PRIMER_RIGHT_3_PENALTY=0.788233
4028
PRIMER_LEFT_3_SEQUENCE=CCCAGCAAGGAATGTCATG
4029
PRIMER_RIGHT_3_SEQUENCE=ACAGTTCCAAGAGTACGGCC
4030
PRIMER_LEFT_3=108,19
4031
PRIMER_RIGHT_3=277,20
4032
PRIMER_LEFT_3_TM=60.056
4033
PRIMER_RIGHT_3_TM=59.212
4034
PRIMER_LEFT_3_GC_PERCENT=52.632
4035
PRIMER_RIGHT_3_GC_PERCENT=55.000
4036
PRIMER_LEFT_3_SELF_ANY=5.00
4037
PRIMER_RIGHT_3_SELF_ANY=4.00
4038
PRIMER_LEFT_3_SELF_END=4.00
4039
PRIMER_RIGHT_3_SELF_END=4.00
4040
PRIMER_LEFT_3_END_STABILITY=6.9000
4041
PRIMER_RIGHT_3_END_STABILITY=12.9000
4042
PRIMER_PAIR_3_COMPL_ANY=5.00
4043
PRIMER_PAIR_3_COMPL_END=1.00
4044
PRIMER_PRODUCT_SIZE_3=170
4045
PRIMER_PAIR_PENALTY_4=2.2372
4046
PRIMER_LEFT_4_PENALTY=1.055809
4047
PRIMER_RIGHT_4_PENALTY=1.181407
4048
PRIMER_LEFT_4_SEQUENCE=CCCAGCAAGGAATGTCATG
4049
PRIMER_RIGHT_4_SEQUENCE=ACAGTTCCAAGAGTACGGCCT
4050
PRIMER_LEFT_4=108,19
4051
PRIMER_RIGHT_4=277,21
4052
PRIMER_LEFT_4_TM=60.056
4053
PRIMER_RIGHT_4_TM=60.181
4054
PRIMER_LEFT_4_GC_PERCENT=52.632
4055
PRIMER_RIGHT_4_GC_PERCENT=52.381
4056
PRIMER_LEFT_4_SELF_ANY=5.00
4057
PRIMER_RIGHT_4_SELF_ANY=4.00
4058
PRIMER_LEFT_4_SELF_END=4.00
4059
PRIMER_RIGHT_4_SELF_END=2.00
4060
PRIMER_LEFT_4_END_STABILITY=6.9000
4061
PRIMER_RIGHT_4_END_STABILITY=10.9000
4062
PRIMER_PAIR_4_COMPL_ANY=5.00
4063
PRIMER_PAIR_4_COMPL_END=1.00
4064
PRIMER_PRODUCT_SIZE_4=170
4065
=
4066
MARKER_NAME=40.RHAP33FD4.seq
4067
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCTCCGAAAAAAAGAAAAGAAAAAAAATTTAGTGATGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCACAGATATCAGATTTTAATATGTGCTCTTCTTACTCACACTCTTCCACCTACCTTTCCTCCCTGGCGAACTTCTGCCCGGCTACTCTCTCCTCATTCCCACCCCAGATAATAGGTCTTTCCTTGCCTCTGTGCCTCTATGGCAATATGAATCTAGTTCTATTCTAACATGCTTTTGTTGATAATCATGCTTTGGGGGTATCTTCCATCCTGTCCACAAGCCTTTTCCCATAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
4068
INCLUDED_REGION=23,305
4069
TARGET=61,32,TG
4070
PRIMER_COMMENT=CL is TCCGAAAAAAAGAAAAGAAAAAAA
4071
PRIMER_COMMENT=CR is AGGGAGGAAAGGTAGGTGGA
4072
PRIMER_PAIR_PENALTY=4.3940
4073
PRIMER_LEFT_PENALTY=4.322131
4074
PRIMER_RIGHT_PENALTY=0.071885
4075
PRIMER_LEFT_SEQUENCE=TCCGAAAAAAAGAAAAGAAAAAAA
4076
PRIMER_RIGHT_SEQUENCE=AGGGAGGAAAGGTAGGTGGA
4077
PRIMER_LEFT=24,24
4078
PRIMER_RIGHT=158,20
4079
PRIMER_LEFT_TM=59.678
4080
PRIMER_RIGHT_TM=59.928
4081
PRIMER_LEFT_GC_PERCENT=20.833
4082
PRIMER_RIGHT_GC_PERCENT=55.000
4083
PRIMER_LEFT_SELF_ANY=3.00
4084
PRIMER_RIGHT_SELF_ANY=2.00
4085
PRIMER_LEFT_SELF_END=0.00
4086
PRIMER_RIGHT_SELF_END=0.00
4087
PRIMER_LEFT_END_STABILITY=7.6000
4088
PRIMER_RIGHT_END_STABILITY=7.9000
4089
PRIMER_PAIR_COMPL_ANY=3.00
4090
PRIMER_PAIR_COMPL_END=0.00
4091
PRIMER_PRODUCT_SIZE=135
4092
PRIMER_PAIR_PENALTY_1=4.5082
4093
PRIMER_LEFT_1_PENALTY=4.436291
4094
PRIMER_RIGHT_1_PENALTY=0.071885
4095
PRIMER_LEFT_1_SEQUENCE=TCCGAAAAAAAGAAAAGAAAAAA
4096
PRIMER_RIGHT_1_SEQUENCE=AGGGAGGAAAGGTAGGTGGA
4097
PRIMER_LEFT_1=24,23
4098
PRIMER_RIGHT_1=158,20
4099
PRIMER_LEFT_1_TM=58.564
4100
PRIMER_RIGHT_1_TM=59.928
4101
PRIMER_LEFT_1_GC_PERCENT=21.739
4102
PRIMER_RIGHT_1_GC_PERCENT=55.000
4103
PRIMER_LEFT_1_SELF_ANY=3.00
4104
PRIMER_RIGHT_1_SELF_ANY=2.00
4105
PRIMER_LEFT_1_SELF_END=0.00
4106
PRIMER_RIGHT_1_SELF_END=0.00
4107
PRIMER_LEFT_1_END_STABILITY=7.6000
4108
PRIMER_RIGHT_1_END_STABILITY=7.9000
4109
PRIMER_PAIR_1_COMPL_ANY=3.00
4110
PRIMER_PAIR_1_COMPL_END=0.00
4111
PRIMER_PRODUCT_SIZE_1=135
4112
PRIMER_PAIR_PENALTY_2=4.6204
4113
PRIMER_LEFT_2_PENALTY=4.322131
4114
PRIMER_RIGHT_2_PENALTY=0.298276
4115
PRIMER_LEFT_2_SEQUENCE=TCCGAAAAAAAGAAAAGAAAAAAA
4116
PRIMER_RIGHT_2_SEQUENCE=GGGAGGAAAGGTAGGTGGAA
4117
PRIMER_LEFT_2=24,24
4118
PRIMER_RIGHT_2=157,20
4119
PRIMER_LEFT_2_TM=59.678
4120
PRIMER_RIGHT_2_TM=60.298
4121
PRIMER_LEFT_2_GC_PERCENT=20.833
4122
PRIMER_RIGHT_2_GC_PERCENT=55.000
4123
PRIMER_LEFT_2_SELF_ANY=3.00
4124
PRIMER_RIGHT_2_SELF_ANY=2.00
4125
PRIMER_LEFT_2_SELF_END=0.00
4126
PRIMER_RIGHT_2_SELF_END=0.00
4127
PRIMER_LEFT_2_END_STABILITY=7.6000
4128
PRIMER_RIGHT_2_END_STABILITY=8.5000
4129
PRIMER_PAIR_2_COMPL_ANY=3.00
4130
PRIMER_PAIR_2_COMPL_END=0.00
4131
PRIMER_PRODUCT_SIZE_2=134
4132
PRIMER_PAIR_PENALTY_3=4.6935
4133
PRIMER_LEFT_3_PENALTY=4.621612
4134
PRIMER_RIGHT_3_PENALTY=0.071885
4135
PRIMER_LEFT_3_SEQUENCE=CTCCGAAAAAAAGAAAAGAAAAAA
4136
PRIMER_RIGHT_3_SEQUENCE=AGGGAGGAAAGGTAGGTGGA
4137
PRIMER_LEFT_3=23,24
4138
PRIMER_RIGHT_3=158,20
4139
PRIMER_LEFT_3_TM=59.378
4140
PRIMER_RIGHT_3_TM=59.928
4141
PRIMER_LEFT_3_GC_PERCENT=25.000
4142
PRIMER_RIGHT_3_GC_PERCENT=55.000
4143
PRIMER_LEFT_3_SELF_ANY=3.00
4144
PRIMER_RIGHT_3_SELF_ANY=2.00
4145
PRIMER_LEFT_3_SELF_END=0.00
4146
PRIMER_RIGHT_3_SELF_END=0.00
4147
PRIMER_LEFT_3_END_STABILITY=7.6000
4148
PRIMER_RIGHT_3_END_STABILITY=7.9000
4149
PRIMER_PAIR_3_COMPL_ANY=4.00
4150
PRIMER_PAIR_3_COMPL_END=0.00
4151
PRIMER_PRODUCT_SIZE_3=136
4152
PRIMER_PAIR_PENALTY_4=4.7251
4153
PRIMER_LEFT_4_PENALTY=4.322131
4154
PRIMER_RIGHT_4_PENALTY=0.402961
4155
PRIMER_LEFT_4_SEQUENCE=TCCGAAAAAAAGAAAAGAAAAAAA
4156
PRIMER_RIGHT_4_SEQUENCE=AGAGTAGCCGGGCAGAAGTT
4157
PRIMER_LEFT_4=24,24
4158
PRIMER_RIGHT_4=182,20
4159
PRIMER_LEFT_4_TM=59.678
4160
PRIMER_RIGHT_4_TM=60.403
4161
PRIMER_LEFT_4_GC_PERCENT=20.833
4162
PRIMER_RIGHT_4_GC_PERCENT=55.000
4163
PRIMER_LEFT_4_SELF_ANY=3.00
4164
PRIMER_RIGHT_4_SELF_ANY=5.00
4165
PRIMER_LEFT_4_SELF_END=0.00
4166
PRIMER_RIGHT_4_SELF_END=3.00
4167
PRIMER_LEFT_4_END_STABILITY=7.6000
4168
PRIMER_RIGHT_4_END_STABILITY=6.7000
4169
PRIMER_PAIR_4_COMPL_ANY=3.00
4170
PRIMER_PAIR_4_COMPL_END=2.00
4171
PRIMER_PRODUCT_SIZE_4=159
4172
=
4173
MARKER_NAME=40.RHAP33FH4.seq
4174
SEQUENCE=NNNNNNNNNNNNNNNCTCCCACCTTCCTCATGTTGCCTTGCTCCAGTGGACAGCCAATTATGACAGATGATGGTGGAGGGACAAGATGTGCCCATATCCCCTCTCCCTTCATATCTTCTACTTCAGCAAGGGAGAGGCTAGCAGAGGGGGTTGAGTGTGTGTTCAGTAAGAAGGGACAGAGTAGTGCTAATGTGGGTGGCTTTCCCCACTGTTCTGGGGAAGACTGAANNNNNNNNNNNNNNNNNNNNNNTACAGAACTCAAAGTCTTTAATTCTGTGATGCATGGTAGAATTCATAGTAATGATAGAAAAATGGTTATTTTATTTTCTGTTCTAGNANGTNGGAAGGCATCGTGAACNAGTTTGNAGGAAAAAAGANCATT
4175
INCLUDED_REGION=15,367
4176
TARGET=228,22,GT
4177
PRIMER_COMMENT=CL is AAGGGAGAGGCTAGCAGAGG
4178
PRIMER_COMMENT=CR is TGAATTCTACCATGCATCACAG
4179
PRIMER_PAIR_PENALTY=3.4589
4180
PRIMER_LEFT_PENALTY=0.110131
4181
PRIMER_RIGHT_PENALTY=3.348806
4182
PRIMER_LEFT_SEQUENCE=AAGGGAGAGGCTAGCAGAGG
4183
PRIMER_RIGHT_SEQUENCE=TGAATTCTACCATGCATCACAG
4184
PRIMER_LEFT=127,20
4185
PRIMER_RIGHT=294,22
4186
PRIMER_LEFT_TM=60.110
4187
PRIMER_RIGHT_TM=58.651
4188
PRIMER_LEFT_GC_PERCENT=60.000
4189
PRIMER_RIGHT_GC_PERCENT=40.909
4190
PRIMER_LEFT_SELF_ANY=6.00
4191
PRIMER_RIGHT_SELF_ANY=6.00
4192
PRIMER_LEFT_SELF_END=0.00
4193
PRIMER_RIGHT_SELF_END=4.00
4194
PRIMER_LEFT_END_STABILITY=7.9000
4195
PRIMER_RIGHT_END_STABILITY=6.7000
4196
PRIMER_PAIR_COMPL_ANY=4.00
4197
PRIMER_PAIR_COMPL_END=0.00
4198
PRIMER_PRODUCT_SIZE=168
4199
PRIMER_PAIR_PENALTY_1=3.4881
4200
PRIMER_LEFT_1_PENALTY=0.139259
4201
PRIMER_RIGHT_1_PENALTY=3.348806
4202
PRIMER_LEFT_1_SEQUENCE=CTAGCAGAGGGGGTTGAGTG
4203
PRIMER_RIGHT_1_SEQUENCE=TGAATTCTACCATGCATCACAG
4204
PRIMER_LEFT_1=137,20
4205
PRIMER_RIGHT_1=294,22
4206
PRIMER_LEFT_1_TM=59.861
4207
PRIMER_RIGHT_1_TM=58.651
4208
PRIMER_LEFT_1_GC_PERCENT=60.000
4209
PRIMER_RIGHT_1_GC_PERCENT=40.909
4210
PRIMER_LEFT_1_SELF_ANY=4.00
4211
PRIMER_RIGHT_1_SELF_ANY=6.00
4212
PRIMER_LEFT_1_SELF_END=0.00
4213
PRIMER_RIGHT_1_SELF_END=4.00
4214
PRIMER_LEFT_1_END_STABILITY=6.4000
4215
PRIMER_RIGHT_1_END_STABILITY=6.7000
4216
PRIMER_PAIR_1_COMPL_ANY=4.00
4217
PRIMER_PAIR_1_COMPL_END=1.00
4218
PRIMER_PRODUCT_SIZE_1=158
4219
PRIMER_PAIR_PENALTY_2=3.4899
4220
PRIMER_LEFT_2_PENALTY=0.141085
4221
PRIMER_RIGHT_2_PENALTY=3.348806
4222
PRIMER_LEFT_2_SEQUENCE=AGGGGGTTGAGTGTGTGTTC
4223
PRIMER_RIGHT_2_SEQUENCE=TGAATTCTACCATGCATCACAG
4224
PRIMER_LEFT_2=144,20
4225
PRIMER_RIGHT_2=294,22
4226
PRIMER_LEFT_2_TM=59.859
4227
PRIMER_RIGHT_2_TM=58.651
4228
PRIMER_LEFT_2_GC_PERCENT=55.000
4229
PRIMER_RIGHT_2_GC_PERCENT=40.909
4230
PRIMER_LEFT_2_SELF_ANY=2.00
4231
PRIMER_RIGHT_2_SELF_ANY=6.00
4232
PRIMER_LEFT_2_SELF_END=0.00
4233
PRIMER_RIGHT_2_SELF_END=4.00
4234
PRIMER_LEFT_2_END_STABILITY=6.7000
4235
PRIMER_RIGHT_2_END_STABILITY=6.7000
4236
PRIMER_PAIR_2_COMPL_ANY=4.00
4237
PRIMER_PAIR_2_COMPL_END=2.00
4238
PRIMER_PRODUCT_SIZE_2=151
4239
PRIMER_PAIR_PENALTY_3=3.4899
4240
PRIMER_LEFT_3_PENALTY=0.141085
4241
PRIMER_RIGHT_3_PENALTY=3.348806
4242
PRIMER_LEFT_3_SEQUENCE=GAGGGGGTTGAGTGTGTGTT
4243
PRIMER_RIGHT_3_SEQUENCE=TGAATTCTACCATGCATCACAG
4244
PRIMER_LEFT_3=143,20
4245
PRIMER_RIGHT_3=294,22
4246
PRIMER_LEFT_3_TM=59.859
4247
PRIMER_RIGHT_3_TM=58.651
4248
PRIMER_LEFT_3_GC_PERCENT=55.000
4249
PRIMER_RIGHT_3_GC_PERCENT=40.909
4250
PRIMER_LEFT_3_SELF_ANY=1.00
4251
PRIMER_RIGHT_3_SELF_ANY=6.00
4252
PRIMER_LEFT_3_SELF_END=0.00
4253
PRIMER_RIGHT_3_SELF_END=4.00
4254
PRIMER_LEFT_3_END_STABILITY=6.4000
4255
PRIMER_RIGHT_3_END_STABILITY=6.7000
4256
PRIMER_PAIR_3_COMPL_ANY=4.00
4257
PRIMER_PAIR_3_COMPL_END=3.00
4258
PRIMER_PRODUCT_SIZE_3=152
4259
PRIMER_PAIR_PENALTY_4=3.5544
4260
PRIMER_LEFT_4_PENALTY=0.110131
4261
PRIMER_RIGHT_4_PENALTY=3.444274
4262
PRIMER_LEFT_4_SEQUENCE=AAGGGAGAGGCTAGCAGAGG
4263
PRIMER_RIGHT_4_SEQUENCE=TGAATTCTACCATGCATCACA
4264
PRIMER_LEFT_4=127,20
4265
PRIMER_RIGHT_4=294,21
4266
PRIMER_LEFT_4_TM=60.110
4267
PRIMER_RIGHT_4_TM=57.556
4268
PRIMER_LEFT_4_GC_PERCENT=60.000
4269
PRIMER_RIGHT_4_GC_PERCENT=38.095
4270
PRIMER_LEFT_4_SELF_ANY=6.00
4271
PRIMER_RIGHT_4_SELF_ANY=6.00
4272
PRIMER_LEFT_4_SELF_END=0.00
4273
PRIMER_RIGHT_4_SELF_END=2.00
4274
PRIMER_LEFT_4_END_STABILITY=7.9000
4275
PRIMER_RIGHT_4_END_STABILITY=6.7000
4276
PRIMER_PAIR_4_COMPL_ANY=4.00
4277
PRIMER_PAIR_4_COMPL_END=1.00
4278
PRIMER_PRODUCT_SIZE_4=168
4279
=
4280
MARKER_NAME=40.RHAP34FH4.seq
4281
SEQUENCE=NNNNNNNNNNNNNNNNNNNCAACTGAGGCTTCTACTGTGACTGCATTAAAGCTCAGCTTGTTCCCTCTGCTTCCATGGGTTTTTATTCCCCCAAAGCAACATTTGCTATACCTACCTCTGGTCTTTAACTGGTATCAGCAGTCTCAGAACAGCAACCCGAGGTTAGGGTGTTACGCCTAGCTAGCCCCGCTGGCAGTAAAGAACCCACGACAGTGTGGGTGGGGCCAGCTATTTCCAAATGCCATGTGGTACAGAAGGTAGGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
4282
INCLUDED_REGION=19,283
4283
TARGET=264,38,AC
4284
PRIMER_COMMENT=CL is
4285
PRIMER_COMMENT=CR is
4286
=
4287
MARKER_NAME=41.RHAP33FD5.seq
4288
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNCTACAGTGTTTGGAGTTTGTCCTGACAGGTTTCTATTTTGCTCTGATGTAATATTTATTCATTATATCCCCTTTGCTGCCTTTATAACAGTTATGTGTATTCTGTGCCATCATCTGGTAGAAGTATGTCATTTGCTTTTTTAATTTTGATTTTACAGGGTATTATGATTAAAAGCCTGGAGTATCAGAAGACTCTGGACTTTTTAAACTGTACTGGATCAAATGCCTTTTGTNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
4289
INCLUDED_REGION=22,248
4290
TARGET=254,15,TA
4291
PRIMER_COMMENT=CL is
4292
PRIMER_COMMENT=CR is
4293
=
4294
MARKER_NAME=41.RHAP33FH5.seq
4295
SEQUENCE=NNNNNNNNNNNNNNTGACCAATCAGCATGTTTTATGTTAAGTTGAAAAGACACTGTGTTGTATGGATTACAGATTTGCTAGGTGGTTAAATATTCTTGGATTTTGGTAACACAGAGGCACATGTGCACGTGCACACATATAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGGATGGGGGGTGTTAATGGGGGAGGGAGAAAGNAGGAAGCAAGAAGCNATCCCTAACTGTAGTGAGGCAGGTAATGNAGAAAAGTGTAACAGATACCGACCTTTAGACAAGACTGCAGATATTAGANAAGCGATGGCTCTGTAATATAAATTTGTTAAGACATAGGGTAGNNNNNNNNNNNNNNNNNNNNNNNNNNN
4296
INCLUDED_REGION=14,343
4297
TARGET=142,42,AC
4298
PRIMER_COMMENT=CL is CTTGGATTTTGGTAACACAGAGG
4299
PRIMER_COMMENT=CR is CTTTCTCCCTCCCCCATTAA
4300
PRIMER_PAIR_PENALTY=3.3360
4301
PRIMER_LEFT_PENALTY=3.085878
4302
PRIMER_RIGHT_PENALTY=0.250137
4303
PRIMER_LEFT_SEQUENCE=CTTGGATTTTGGTAACACAGAGG
4304
PRIMER_RIGHT_SEQUENCE=CTTTCTCCCTCCCCCATTAA
4305
PRIMER_LEFT=94,23
4306
PRIMER_RIGHT=218,20
4307
PRIMER_LEFT_TM=59.914
4308
PRIMER_RIGHT_TM=60.250
4309
PRIMER_LEFT_GC_PERCENT=43.478
4310
PRIMER_RIGHT_GC_PERCENT=50.000
4311
PRIMER_LEFT_SELF_ANY=4.00
4312
PRIMER_RIGHT_SELF_ANY=4.00
4313
PRIMER_LEFT_SELF_END=0.00
4314
PRIMER_RIGHT_SELF_END=4.00
4315
PRIMER_LEFT_END_STABILITY=7.9000
4316
PRIMER_RIGHT_END_STABILITY=6.2000
4317
PRIMER_PAIR_COMPL_ANY=4.00
4318
PRIMER_PAIR_COMPL_END=0.00
4319
PRIMER_PRODUCT_SIZE=125
4320
PRIMER_PAIR_PENALTY_1=3.4933
4321
PRIMER_LEFT_1_PENALTY=3.369403
4322
PRIMER_RIGHT_1_PENALTY=0.123924
4323
PRIMER_LEFT_1_SEQUENCE=TGGATTACAGATTTGCTAGGTGG
4324
PRIMER_RIGHT_1_SEQUENCE=TTTCTCCCTCCCCCATTAAC
4325
PRIMER_LEFT_1=62,23
4326
PRIMER_RIGHT_1=217,20
4327
PRIMER_LEFT_1_TM=60.369
4328
PRIMER_RIGHT_1_TM=60.124
4329
PRIMER_LEFT_1_GC_PERCENT=43.478
4330
PRIMER_RIGHT_1_GC_PERCENT=50.000
4331
PRIMER_LEFT_1_SELF_ANY=4.00
4332
PRIMER_RIGHT_1_SELF_ANY=4.00
4333
PRIMER_LEFT_1_SELF_END=0.00
4334
PRIMER_RIGHT_1_SELF_END=2.00
4335
PRIMER_LEFT_1_END_STABILITY=9.4000
4336
PRIMER_RIGHT_1_END_STABILITY=6.0000
4337
PRIMER_PAIR_1_COMPL_ANY=3.00
4338
PRIMER_PAIR_1_COMPL_END=2.00
4339
PRIMER_PRODUCT_SIZE_1=156
4340
PRIMER_PAIR_PENALTY_2=3.5023
4341
PRIMER_LEFT_2_PENALTY=1.375502
4342
PRIMER_RIGHT_2_PENALTY=2.126755
4343
PRIMER_LEFT_2_SEQUENCE=TTGGTAACACAGAGGCACATG
4344
PRIMER_RIGHT_2_SEQUENCE=CCTGCCTCACTACAGTTAGGG
4345
PRIMER_LEFT_2=102,21
4346
PRIMER_RIGHT_2=257,21
4347
PRIMER_LEFT_2_TM=59.624
4348
PRIMER_RIGHT_2_TM=58.873
4349
PRIMER_LEFT_2_GC_PERCENT=47.619
4350
PRIMER_RIGHT_2_GC_PERCENT=57.143
4351
PRIMER_LEFT_2_SELF_ANY=4.00
4352
PRIMER_RIGHT_2_SELF_ANY=8.00
4353
PRIMER_LEFT_2_SELF_END=4.00
4354
PRIMER_RIGHT_2_SELF_END=6.00
4355
PRIMER_LEFT_2_END_STABILITY=6.6000
4356
PRIMER_RIGHT_2_END_STABILITY=8.7000
4357
PRIMER_PAIR_2_COMPL_ANY=6.00
4358
PRIMER_PAIR_2_COMPL_END=0.00
4359
PRIMER_PRODUCT_SIZE_2=156
4360
PRIMER_PAIR_PENALTY_3=3.6195
4361
PRIMER_LEFT_3_PENALTY=3.369403
4362
PRIMER_RIGHT_3_PENALTY=0.250137
4363
PRIMER_LEFT_3_SEQUENCE=TGGATTACAGATTTGCTAGGTGG
4364
PRIMER_RIGHT_3_SEQUENCE=CTTTCTCCCTCCCCCATTAA
4365
PRIMER_LEFT_3=62,23
4366
PRIMER_RIGHT_3=218,20
4367
PRIMER_LEFT_3_TM=60.369
4368
PRIMER_RIGHT_3_TM=60.250
4369
PRIMER_LEFT_3_GC_PERCENT=43.478
4370
PRIMER_RIGHT_3_GC_PERCENT=50.000
4371
PRIMER_LEFT_3_SELF_ANY=4.00
4372
PRIMER_RIGHT_3_SELF_ANY=4.00
4373
PRIMER_LEFT_3_SELF_END=0.00
4374
PRIMER_RIGHT_3_SELF_END=4.00
4375
PRIMER_LEFT_3_END_STABILITY=9.4000
4376
PRIMER_RIGHT_3_END_STABILITY=6.2000
4377
PRIMER_PAIR_3_COMPL_ANY=3.00
4378
PRIMER_PAIR_3_COMPL_END=2.00
4379
PRIMER_PRODUCT_SIZE_3=157
4380
PRIMER_PAIR_PENALTY_4=3.6769
4381
PRIMER_LEFT_4_PENALTY=1.375502
4382
PRIMER_RIGHT_4_PENALTY=2.301367
4383
PRIMER_LEFT_4_SEQUENCE=TTGGTAACACAGAGGCACATG
4384
PRIMER_RIGHT_4_SEQUENCE=ACCTGCCTCACTACAGTTAGGG
4385
PRIMER_LEFT_4=102,21
4386
PRIMER_RIGHT_4=258,22
4387
PRIMER_LEFT_4_TM=59.624
4388
PRIMER_RIGHT_4_TM=59.699
4389
PRIMER_LEFT_4_GC_PERCENT=47.619
4390
PRIMER_RIGHT_4_GC_PERCENT=54.545
4391
PRIMER_LEFT_4_SELF_ANY=4.00
4392
PRIMER_RIGHT_4_SELF_ANY=8.00
4393
PRIMER_LEFT_4_SELF_END=4.00
4394
PRIMER_RIGHT_4_SELF_END=6.00
4395
PRIMER_LEFT_4_END_STABILITY=6.6000
4396
PRIMER_RIGHT_4_END_STABILITY=8.7000
4397
PRIMER_PAIR_4_COMPL_ANY=6.00
4398
PRIMER_PAIR_4_COMPL_END=0.00
4399
PRIMER_PRODUCT_SIZE_4=157
4400
=
4401
MARKER_NAME=41.RHAP34FD5.seq
4402
SEQUENCE=NNNNNNNNNNNNNNNNNNNATATATTCTATGTATCTATGCNNNNNNNNNNNNNNNNNNNNAATTTATTTATTATTTTACCTTTGGGAAATACTCTCTTTGCAAAGCTTATAATGACTCCATGATAATTAAAAGCAGCAGGGGTTCAAGAGGCAAGAATGCATCTAGGTCTTAGCAAAATGGCAAATGCTGAGACCTTCAAGACCGTCATTAAGGCTGTGGGAATGAACTCTGAAAACATGTAGTTCAAATATAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
4403
INCLUDED_REGION=19,236
4404
TARGET=40,20,AT
4405
PRIMER_COMMENT=CL is
4406
PRIMER_COMMENT=CR is
4407
=
4408
MARKER_NAME=41.RHAP34FH5.seq
4409
SEQUENCE=NNNNNNNNNNNNNNNNNNCAGGCATCTCTGAGGGAAAGCAGGAGAAGGAGTCTAATTCCTCTGCAAACTAATTAATGAGCCTTCCCAGAGTGCTGGCAGCTAACAGGCAAAACTGAGAACCACTAACAGCAAGTACACCAAAGCCAGTAAGTAGCAATGCTTTCCAGGTGTGTGCATGTATACCNNNNNNNNNNNNNNNNNNNNNNNNNNCTAGAGCACCCATGCATGTCATAGTGTGCATGGAGGTCAGAGGACAGCCCTGCCTTCTGCTCTTTGCCTTTCACTCTGTTTCAGACAGAC
4410
INCLUDED_REGION=18,282
4411
TARGET=184,26,TG
4412
PRIMER_COMMENT=CL is CAGCAAGTACACCAAAGCCA
4413
PRIMER_COMMENT=CR is GTCCTCTGACCTCCATGCAC
4414
PRIMER_PAIR_PENALTY=0.7845
4415
PRIMER_LEFT_PENALTY=0.095506
4416
PRIMER_RIGHT_PENALTY=0.688966
4417
PRIMER_LEFT_SEQUENCE=CAGCAAGTACACCAAAGCCA
4418
PRIMER_RIGHT_SEQUENCE=GTCCTCTGACCTCCATGCAC
4419
PRIMER_LEFT=126,20
4420
PRIMER_RIGHT=254,20
4421
PRIMER_LEFT_TM=59.904
4422
PRIMER_RIGHT_TM=60.689
4423
PRIMER_LEFT_GC_PERCENT=50.000
4424
PRIMER_RIGHT_GC_PERCENT=60.000
4425
PRIMER_LEFT_SELF_ANY=4.00
4426
PRIMER_RIGHT_SELF_ANY=4.00
4427
PRIMER_LEFT_SELF_END=0.00
4428
PRIMER_RIGHT_SELF_END=2.00
4429
PRIMER_LEFT_END_STABILITY=9.7000
4430
PRIMER_RIGHT_END_STABILITY=8.2000
4431
PRIMER_PAIR_COMPL_ANY=3.00
4432
PRIMER_PAIR_COMPL_END=2.00
4433
PRIMER_PRODUCT_SIZE=129
4434
PRIMER_PAIR_PENALTY_1=0.7949
4435
PRIMER_LEFT_1_PENALTY=0.347100
4436
PRIMER_RIGHT_1_PENALTY=0.447804
4437
PRIMER_LEFT_1_SEQUENCE=CAGAGTGCTGGCAGCTAACA
4438
PRIMER_RIGHT_1_SEQUENCE=CTATGACATGCATGGGTGCT
4439
PRIMER_LEFT_1=85,20
4440
PRIMER_RIGHT_1=233,20
4441
PRIMER_LEFT_1_TM=60.347
4442
PRIMER_RIGHT_1_TM=59.552
4443
PRIMER_LEFT_1_GC_PERCENT=55.000
4444
PRIMER_RIGHT_1_GC_PERCENT=50.000
4445
PRIMER_LEFT_1_SELF_ANY=7.00
4446
PRIMER_RIGHT_1_SELF_ANY=8.00
4447
PRIMER_LEFT_1_SELF_END=5.00
4448
PRIMER_RIGHT_1_SELF_END=2.00
4449
PRIMER_LEFT_1_END_STABILITY=6.0000
4450
PRIMER_RIGHT_1_END_STABILITY=7.9000
4451
PRIMER_PAIR_1_COMPL_ANY=4.00
4452
PRIMER_PAIR_1_COMPL_END=2.00
4453
PRIMER_PRODUCT_SIZE_1=149
4454
PRIMER_PAIR_PENALTY_2=0.8606
4455
PRIMER_LEFT_2_PENALTY=0.347100
4456
PRIMER_RIGHT_2_PENALTY=0.513452
4457
PRIMER_LEFT_2_SEQUENCE=CAGAGTGCTGGCAGCTAACA
4458
PRIMER_RIGHT_2_SEQUENCE=TATGACATGCATGGGTGCTC
4459
PRIMER_LEFT_2=85,20
4460
PRIMER_RIGHT_2=232,20
4461
PRIMER_LEFT_2_TM=60.347
4462
PRIMER_RIGHT_2_TM=60.513
4463
PRIMER_LEFT_2_GC_PERCENT=55.000
4464
PRIMER_RIGHT_2_GC_PERCENT=50.000
4465
PRIMER_LEFT_2_SELF_ANY=7.00
4466
PRIMER_RIGHT_2_SELF_ANY=8.00
4467
PRIMER_LEFT_2_SELF_END=5.00
4468
PRIMER_RIGHT_2_SELF_END=4.00
4469
PRIMER_LEFT_2_END_STABILITY=6.0000
4470
PRIMER_RIGHT_2_END_STABILITY=8.2000
4471
PRIMER_PAIR_2_COMPL_ANY=4.00
4472
PRIMER_PAIR_2_COMPL_END=1.00
4473
PRIMER_PRODUCT_SIZE_2=148
4474
PRIMER_PAIR_PENALTY_3=0.9010
4475
PRIMER_LEFT_3_PENALTY=0.095506
4476
PRIMER_RIGHT_3_PENALTY=0.805475
4477
PRIMER_LEFT_3_SEQUENCE=CAGCAAGTACACCAAAGCCA
4478
PRIMER_RIGHT_3_SEQUENCE=GAAAGGCAAAGAGCAGAAGG
4479
PRIMER_LEFT_3=126,20
4480
PRIMER_RIGHT_3=281,20
4481
PRIMER_LEFT_3_TM=59.904
4482
PRIMER_RIGHT_3_TM=59.195
4483
PRIMER_LEFT_3_GC_PERCENT=50.000
4484
PRIMER_RIGHT_3_GC_PERCENT=50.000
4485
PRIMER_LEFT_3_SELF_ANY=4.00
4486
PRIMER_RIGHT_3_SELF_ANY=2.00
4487
PRIMER_LEFT_3_SELF_END=0.00
4488
PRIMER_RIGHT_3_SELF_END=0.00
4489
PRIMER_LEFT_3_END_STABILITY=9.7000
4490
PRIMER_RIGHT_3_END_STABILITY=8.2000
4491
PRIMER_PAIR_3_COMPL_ANY=3.00
4492
PRIMER_PAIR_3_COMPL_END=1.00
4493
PRIMER_PRODUCT_SIZE_3=156
4494
PRIMER_PAIR_PENALTY_4=0.9241
4495
PRIMER_LEFT_4_PENALTY=0.095506
4496
PRIMER_RIGHT_4_PENALTY=0.828636
4497
PRIMER_LEFT_4_SEQUENCE=CAGCAAGTACACCAAAGCCA
4498
PRIMER_RIGHT_4_SEQUENCE=CTGTCCTCTGACCTCCATGC
4499
PRIMER_LEFT_4=126,20
4500
PRIMER_RIGHT_4=256,20
4501
PRIMER_LEFT_4_TM=59.904
4502
PRIMER_RIGHT_4_TM=60.829
4503
PRIMER_LEFT_4_GC_PERCENT=50.000
4504
PRIMER_RIGHT_4_GC_PERCENT=60.000
4505
PRIMER_LEFT_4_SELF_ANY=4.00
4506
PRIMER_RIGHT_4_SELF_ANY=4.00
4507
PRIMER_LEFT_4_SELF_END=0.00
4508
PRIMER_RIGHT_4_SELF_END=2.00
4509
PRIMER_LEFT_4_END_STABILITY=9.7000
4510
PRIMER_RIGHT_4_END_STABILITY=8.4000
4511
PRIMER_PAIR_4_COMPL_ANY=4.00
4512
PRIMER_PAIR_4_COMPL_END=2.00
4513
PRIMER_PRODUCT_SIZE_4=131
4514
=
4515
MARKER_NAME=43.RHAP34FD7.seq
4516
SEQUENCE=ANACCCCACCNCCAGCNCCNCACACCNCAACCAGAACNGGTCNNNCNNTNTNNGTGCNNNNNNNNNNNNNNNNATCNCNCNGTGTGTGCCCNGCTCNGTATCNGTCNGNGTGTCNNGCCTCGCNNNNNNNNNNNNNNNNNNNNNNNNNCTGCGTGTCTCGTCGTGTGTGATTGATGTTGTGNGTCNTGTCGTGATGCTGNTCGTCTCTGATGTGNTNNGNNGNNTGNTGTTGNTAGNTNNTAGTGNNGNTANNGNTNCNNGCTCNTGNTNNNGNTCCNNGNTNNTCANNGANGNNGATNCTCGCTCNTNGNTGNTGATGNNGCACCNGNTGTNGCNGATA
4517
INCLUDED_REGION=0,340
4518
TARGET=59,14,GT
4519
TARGET=124,24,TG
4520
PRIMER_COMMENT=CL is
4521
PRIMER_COMMENT=CR is
4522
=
4523
MARKER_NAME=44.RHAP33FD8.seq
4524
SEQUENCE=NNNNNNNNNNNTGCCATATTTCCCNAGCCATTAACTCATCTGACATAGGCCTTCTTCACGTTGCATCTAGATAGGTCTTCCTGTCTTCCTCTCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAGAGAGAGAGAAGGAGGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
4525
INCLUDED_REGION=11,179
4526
TARGET=95,56,GT
4527
TARGET=174,16,GA
4528
PRIMER_COMMENT=CL is GCCTTCTTCACGTTGCATCT
4529
PRIMER_COMMENT=CR is CCCCCTCCTTCTCTCTCTCT
4530
PRIMER_PAIR_PENALTY=1.4262
4531
PRIMER_LEFT_PENALTY=0.405717
4532
PRIMER_RIGHT_PENALTY=1.020490
4533
PRIMER_LEFT_SEQUENCE=GCCTTCTTCACGTTGCATCT
4534
PRIMER_RIGHT_SEQUENCE=CCCCCTCCTTCTCTCTCTCT
4535
PRIMER_LEFT=48,20
4536
PRIMER_RIGHT=172,20
4537
PRIMER_LEFT_TM=60.406
4538
PRIMER_RIGHT_TM=58.980
4539
PRIMER_LEFT_GC_PERCENT=50.000
4540
PRIMER_RIGHT_GC_PERCENT=60.000
4541
PRIMER_LEFT_SELF_ANY=4.00
4542
PRIMER_RIGHT_SELF_ANY=0.00
4543
PRIMER_LEFT_SELF_END=2.00
4544
PRIMER_RIGHT_SELF_END=0.00
4545
PRIMER_LEFT_END_STABILITY=6.6000
4546
PRIMER_RIGHT_END_STABILITY=6.4000
4547
PRIMER_PAIR_COMPL_ANY=1.00
4548
PRIMER_PAIR_COMPL_END=0.00
4549
PRIMER_PRODUCT_SIZE=125
4550
PRIMER_PAIR_PENALTY_1=1.6607
4551
PRIMER_LEFT_1_PENALTY=0.405717
4552
PRIMER_RIGHT_1_PENALTY=1.254998
4553
PRIMER_LEFT_1_SEQUENCE=GCCTTCTTCACGTTGCATCT
4554
PRIMER_RIGHT_1_SEQUENCE=CCCCCCTCCTTCTCTCTCT
4555
PRIMER_LEFT_1=48,20
4556
PRIMER_RIGHT_1=173,19
4557
PRIMER_LEFT_1_TM=60.406
4558
PRIMER_RIGHT_1_TM=59.745
4559
PRIMER_LEFT_1_GC_PERCENT=50.000
4560
PRIMER_RIGHT_1_GC_PERCENT=63.158
4561
PRIMER_LEFT_1_SELF_ANY=4.00
4562
PRIMER_RIGHT_1_SELF_ANY=0.00
4563
PRIMER_LEFT_1_SELF_END=2.00
4564
PRIMER_RIGHT_1_SELF_END=0.00
4565
PRIMER_LEFT_1_END_STABILITY=6.6000
4566
PRIMER_RIGHT_1_END_STABILITY=6.4000
4567
PRIMER_PAIR_1_COMPL_ANY=1.00
4568
PRIMER_PAIR_1_COMPL_END=0.00
4569
PRIMER_PRODUCT_SIZE_1=126
4570
PRIMER_PAIR_PENALTY_2=1.6804
4571
PRIMER_LEFT_2_PENALTY=0.659953
4572
PRIMER_RIGHT_2_PENALTY=1.020490
4573
PRIMER_LEFT_2_SEQUENCE=ATAGGCCTTCTTCACGTTGC
4574
PRIMER_RIGHT_2_SEQUENCE=CCCCCTCCTTCTCTCTCTCT
4575
PRIMER_LEFT_2=44,20
4576
PRIMER_RIGHT_2=172,20
4577
PRIMER_LEFT_2_TM=59.340
4578
PRIMER_RIGHT_2_TM=58.980
4579
PRIMER_LEFT_2_GC_PERCENT=50.000
4580
PRIMER_RIGHT_2_GC_PERCENT=60.000
4581
PRIMER_LEFT_2_SELF_ANY=6.00
4582
PRIMER_RIGHT_2_SELF_ANY=0.00
4583
PRIMER_LEFT_2_SELF_END=2.00
4584
PRIMER_RIGHT_2_SELF_END=0.00
4585
PRIMER_LEFT_2_END_STABILITY=8.2000
4586
PRIMER_RIGHT_2_END_STABILITY=6.4000
4587
PRIMER_PAIR_2_COMPL_ANY=3.00
4588
PRIMER_PAIR_2_COMPL_END=0.00
4589
PRIMER_PRODUCT_SIZE_2=129
4590
PRIMER_PAIR_PENALTY_3=1.7284
4591
PRIMER_LEFT_3_PENALTY=0.405717
4592
PRIMER_RIGHT_3_PENALTY=1.322707
4593
PRIMER_LEFT_3_SEQUENCE=GCCTTCTTCACGTTGCATCT
4594
PRIMER_RIGHT_3_SEQUENCE=CCCCCTCCTTCTCTCTCTCTT
4595
PRIMER_LEFT_3=48,20
4596
PRIMER_RIGHT_3=172,21
4597
PRIMER_LEFT_3_TM=60.406
4598
PRIMER_RIGHT_3_TM=60.323
4599
PRIMER_LEFT_3_GC_PERCENT=50.000
4600
PRIMER_RIGHT_3_GC_PERCENT=57.143
4601
PRIMER_LEFT_3_SELF_ANY=4.00
4602
PRIMER_RIGHT_3_SELF_ANY=0.00
4603
PRIMER_LEFT_3_SELF_END=2.00
4604
PRIMER_RIGHT_3_SELF_END=0.00
4605
PRIMER_LEFT_3_END_STABILITY=6.6000
4606
PRIMER_RIGHT_3_END_STABILITY=6.7000
4607
PRIMER_PAIR_3_COMPL_ANY=1.00
4608
PRIMER_PAIR_3_COMPL_END=0.00
4609
PRIMER_PRODUCT_SIZE_3=125
4610
PRIMER_PAIR_PENALTY_4=1.9150
4611
PRIMER_LEFT_4_PENALTY=0.659953
4612
PRIMER_RIGHT_4_PENALTY=1.254998
4613
PRIMER_LEFT_4_SEQUENCE=ATAGGCCTTCTTCACGTTGC
4614
PRIMER_RIGHT_4_SEQUENCE=CCCCCCTCCTTCTCTCTCT
4615
PRIMER_LEFT_4=44,20
4616
PRIMER_RIGHT_4=173,19
4617
PRIMER_LEFT_4_TM=59.340
4618
PRIMER_RIGHT_4_TM=59.745
4619
PRIMER_LEFT_4_GC_PERCENT=50.000
4620
PRIMER_RIGHT_4_GC_PERCENT=63.158
4621
PRIMER_LEFT_4_SELF_ANY=6.00
4622
PRIMER_RIGHT_4_SELF_ANY=0.00
4623
PRIMER_LEFT_4_SELF_END=2.00
4624
PRIMER_RIGHT_4_SELF_END=0.00
4625
PRIMER_LEFT_4_END_STABILITY=8.2000
4626
PRIMER_RIGHT_4_END_STABILITY=6.4000
4627
PRIMER_PAIR_4_COMPL_ANY=3.00
4628
PRIMER_PAIR_4_COMPL_END=0.00
4629
PRIMER_PRODUCT_SIZE_4=130
4630
=
4631
MARKER_NAME=44.RHAP34FD8.seq
4632
SEQUENCE=NNNNNNNNNNNACTCCAAATAGTGCCTACATCCCATATTATTCTATCTTTGCTATCAATTGATTTTTTTCACAAAGTTTCTCTTCCTCCCAAATTAGAATGATTTCTCTCCCTTGTTTGGTTGTATGTTCATTCTTAAGCTGTAGTTAACACTGTGCCTTAGAACATCTTATTACAAGATCCCATTTAGCATTTACATAACCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAGCCATGGAGCACTATTATTTGTTGGTAATTGNTTAAATGCAAAAAACAATGTCCCTTG
4633
INCLUDED_REGION=11,284
4634
TARGET=204,30,TG
4635
PRIMER_COMMENT=CL is TTTCTCTCCCTTGTTTGGTTG
4636
PRIMER_COMMENT=CR is CAAATAATAGTGCTCCATGGCTT
4637
PRIMER_PAIR_PENALTY=5.2461
4638
PRIMER_LEFT_PENALTY=1.799253
4639
PRIMER_RIGHT_PENALTY=3.446835
4640
PRIMER_LEFT_SEQUENCE=TTTCTCTCCCTTGTTTGGTTG
4641
PRIMER_RIGHT_SEQUENCE=CAAATAATAGTGCTCCATGGCTT
4642
PRIMER_LEFT=102,21
4643
PRIMER_RIGHT=257,23
4644
PRIMER_LEFT_TM=59.201
4645
PRIMER_RIGHT_TM=59.553
4646
PRIMER_LEFT_GC_PERCENT=42.857
4647
PRIMER_RIGHT_GC_PERCENT=39.130
4648
PRIMER_LEFT_SELF_ANY=2.00
4649
PRIMER_RIGHT_SELF_ANY=6.00
4650
PRIMER_LEFT_SELF_END=0.00
4651
PRIMER_RIGHT_SELF_END=2.00
4652
PRIMER_LEFT_END_STABILITY=8.2000
4653
PRIMER_RIGHT_END_STABILITY=9.7000
4654
PRIMER_PAIR_COMPL_ANY=5.00
4655
PRIMER_PAIR_COMPL_END=0.00
4656
PRIMER_PRODUCT_SIZE=156
4657
PRIMER_PAIR_PENALTY_1=5.2903
4658
PRIMER_LEFT_1_PENALTY=1.843499
4659
PRIMER_RIGHT_1_PENALTY=3.446835
4660
PRIMER_LEFT_1_SEQUENCE=TGATTTCTCTCCCTTGTTTGG
4661
PRIMER_RIGHT_1_SEQUENCE=CAAATAATAGTGCTCCATGGCTT
4662
PRIMER_LEFT_1=99,21
4663
PRIMER_RIGHT_1=257,23
4664
PRIMER_LEFT_1_TM=59.157
4665
PRIMER_RIGHT_1_TM=59.553
4666
PRIMER_LEFT_1_GC_PERCENT=42.857
4667
PRIMER_RIGHT_1_GC_PERCENT=39.130
4668
PRIMER_LEFT_1_SELF_ANY=2.00
4669
PRIMER_RIGHT_1_SELF_ANY=6.00
4670
PRIMER_LEFT_1_SELF_END=0.00
4671
PRIMER_RIGHT_1_SELF_END=2.00
4672
PRIMER_LEFT_1_END_STABILITY=8.8000
4673
PRIMER_RIGHT_1_END_STABILITY=9.7000
4674
PRIMER_PAIR_1_COMPL_ANY=5.00
4675
PRIMER_PAIR_1_COMPL_END=0.00
4676
PRIMER_PRODUCT_SIZE_1=159
4677
PRIMER_PAIR_PENALTY_2=5.3669
4678
PRIMER_LEFT_2_PENALTY=1.799253
4679
PRIMER_RIGHT_2_PENALTY=3.567613
4680
PRIMER_LEFT_2_SEQUENCE=TTTCTCTCCCTTGTTTGGTTG
4681
PRIMER_RIGHT_2_SEQUENCE=AACAAATAATAGTGCTCCATGGC
4682
PRIMER_LEFT_2=102,21
4683
PRIMER_RIGHT_2=259,23
4684
PRIMER_LEFT_2_TM=59.201
4685
PRIMER_RIGHT_2_TM=59.432
4686
PRIMER_LEFT_2_GC_PERCENT=42.857
4687
PRIMER_RIGHT_2_GC_PERCENT=39.130
4688
PRIMER_LEFT_2_SELF_ANY=2.00
4689
PRIMER_RIGHT_2_SELF_ANY=6.00
4690
PRIMER_LEFT_2_SELF_END=0.00
4691
PRIMER_RIGHT_2_SELF_END=4.00
4692
PRIMER_LEFT_2_END_STABILITY=8.2000
4693
PRIMER_RIGHT_2_END_STABILITY=9.6000
4694
PRIMER_PAIR_2_COMPL_ANY=5.00
4695
PRIMER_PAIR_2_COMPL_END=1.00
4696
PRIMER_PRODUCT_SIZE_2=158
4697
PRIMER_PAIR_PENALTY_3=5.4111
4698
PRIMER_LEFT_3_PENALTY=1.843499
4699
PRIMER_RIGHT_3_PENALTY=3.567613
4700
PRIMER_LEFT_3_SEQUENCE=TGATTTCTCTCCCTTGTTTGG
4701
PRIMER_RIGHT_3_SEQUENCE=AACAAATAATAGTGCTCCATGGC
4702
PRIMER_LEFT_3=99,21
4703
PRIMER_RIGHT_3=259,23
4704
PRIMER_LEFT_3_TM=59.157
4705
PRIMER_RIGHT_3_TM=59.432
4706
PRIMER_LEFT_3_GC_PERCENT=42.857
4707
PRIMER_RIGHT_3_GC_PERCENT=39.130
4708
PRIMER_LEFT_3_SELF_ANY=2.00
4709
PRIMER_RIGHT_3_SELF_ANY=6.00
4710
PRIMER_LEFT_3_SELF_END=0.00
4711
PRIMER_RIGHT_3_SELF_END=4.00
4712
PRIMER_LEFT_3_END_STABILITY=8.8000
4713
PRIMER_RIGHT_3_END_STABILITY=9.6000
4714
PRIMER_PAIR_3_COMPL_ANY=5.00
4715
PRIMER_PAIR_3_COMPL_END=1.00
4716
PRIMER_PRODUCT_SIZE_3=161
4717
PRIMER_PAIR_PENALTY_4=5.4590
4718
PRIMER_LEFT_4_PENALTY=2.012153
4719
PRIMER_RIGHT_4_PENALTY=3.446835
4720
PRIMER_LEFT_4_SEQUENCE=TTTCTCTCCCTTGTTTGGTTGT
4721
PRIMER_RIGHT_4_SEQUENCE=CAAATAATAGTGCTCCATGGCTT
4722
PRIMER_LEFT_4=102,22
4723
PRIMER_RIGHT_4=257,23
4724
PRIMER_LEFT_4_TM=60.012
4725
PRIMER_RIGHT_4_TM=59.553
4726
PRIMER_LEFT_4_GC_PERCENT=40.909
4727
PRIMER_RIGHT_4_GC_PERCENT=39.130
4728
PRIMER_LEFT_4_SELF_ANY=2.00
4729
PRIMER_RIGHT_4_SELF_ANY=6.00
4730
PRIMER_LEFT_4_SELF_END=0.00
4731
PRIMER_RIGHT_4_SELF_END=2.00
4732
PRIMER_LEFT_4_END_STABILITY=6.4000
4733
PRIMER_RIGHT_4_END_STABILITY=9.7000
4734
PRIMER_PAIR_4_COMPL_ANY=5.00
4735
PRIMER_PAIR_4_COMPL_END=0.00
4736
PRIMER_PRODUCT_SIZE_4=156
4737
=
4738
MARKER_NAME=45.RHAP33FD9.seq
4739
SEQUENCE=NNNNNNNNNNNCTCGTTATGAAAAATTACATCTAATTGGAAGGTAGTTGAGTGAGTGGAGTGAAGTTGAGTTCAGTTCATTTCAGTGAGTCAGTGCAGTGGAATTGTGTTCATGCAATTCAGTGTAGGTCAGCTGGAGGCCCTTGTCCAGGCCACATGTAATACAGAAACAAAAATAGGGAATAGTACCAAGGAACTCTCACTAGAATAAAAGCAAGGAGTGTCCGCAGCACTGTGTTTATTCCTAATGNNNNNNNNNNNNNNNNGCCTCCCAAGGAGAGTCTGCCATGACCCCAGGTAAGAACTCACGCTGCATATGCCAATCCTTTGCTAGAGCATGTGAGCACCACGCCCACTCGACACAAACTTT
4740
INCLUDED_REGION=11,358
4741
TARGET=249,16,CT
4742
PRIMER_COMMENT=CL is TAAAAGCAAGGAGTGTCCGC
4743
PRIMER_COMMENT=CR is AAAGTTTGTGTCGAGTGGGC
4744
PRIMER_PAIR_PENALTY=0.5426
4745
PRIMER_LEFT_PENALTY=0.386286
4746
PRIMER_RIGHT_PENALTY=0.156287
4747
PRIMER_LEFT_SEQUENCE=TAAAAGCAAGGAGTGTCCGC
4748
PRIMER_RIGHT_SEQUENCE=AAAGTTTGTGTCGAGTGGGC
4749
PRIMER_LEFT=207,20
4750
PRIMER_RIGHT=368,20
4751
PRIMER_LEFT_TM=60.386
4752
PRIMER_RIGHT_TM=60.156
4753
PRIMER_LEFT_GC_PERCENT=50.000
4754
PRIMER_RIGHT_GC_PERCENT=50.000
4755
PRIMER_LEFT_SELF_ANY=3.00
4756
PRIMER_RIGHT_SELF_ANY=5.00
4757
PRIMER_LEFT_SELF_END=2.00
4758
PRIMER_RIGHT_SELF_END=2.00
4759
PRIMER_LEFT_END_STABILITY=11.4000
4760
PRIMER_RIGHT_END_STABILITY=11.2000
4761
PRIMER_PAIR_COMPL_ANY=3.00
4762
PRIMER_PAIR_COMPL_END=2.00
4763
PRIMER_PRODUCT_SIZE=162
4764
PRIMER_PAIR_PENALTY_1=0.5543
4765
PRIMER_LEFT_1_PENALTY=0.386286
4766
PRIMER_RIGHT_1_PENALTY=0.168025
4767
PRIMER_LEFT_1_SEQUENCE=TAAAAGCAAGGAGTGTCCGC
4768
PRIMER_RIGHT_1_SEQUENCE=TGGTGCTCACATGCTCTAGC
4769
PRIMER_LEFT_1=207,20
4770
PRIMER_RIGHT_1=347,20
4771
PRIMER_LEFT_1_TM=60.386
4772
PRIMER_RIGHT_1_TM=60.168
4773
PRIMER_LEFT_1_GC_PERCENT=50.000
4774
PRIMER_RIGHT_1_GC_PERCENT=55.000
4775
PRIMER_LEFT_1_SELF_ANY=3.00
4776
PRIMER_RIGHT_1_SELF_ANY=4.00
4777
PRIMER_LEFT_1_SELF_END=2.00
4778
PRIMER_RIGHT_1_SELF_END=4.00
4779
PRIMER_LEFT_1_END_STABILITY=11.4000
4780
PRIMER_RIGHT_1_END_STABILITY=7.2000
4781
PRIMER_PAIR_1_COMPL_ANY=5.00
4782
PRIMER_PAIR_1_COMPL_END=3.00
4783
PRIMER_PRODUCT_SIZE_1=141
4784
PRIMER_PAIR_PENALTY_2=0.5543
4785
PRIMER_LEFT_2_PENALTY=0.386286
4786
PRIMER_RIGHT_2_PENALTY=0.168025
4787
PRIMER_LEFT_2_SEQUENCE=TAAAAGCAAGGAGTGTCCGC
4788
PRIMER_RIGHT_2_SEQUENCE=GGTGCTCACATGCTCTAGCA
4789
PRIMER_LEFT_2=207,20
4790
PRIMER_RIGHT_2=346,20
4791
PRIMER_LEFT_2_TM=60.386
4792
PRIMER_RIGHT_2_TM=60.168
4793
PRIMER_LEFT_2_GC_PERCENT=50.000
4794
PRIMER_RIGHT_2_GC_PERCENT=55.000
4795
PRIMER_LEFT_2_SELF_ANY=3.00
4796
PRIMER_RIGHT_2_SELF_ANY=6.00
4797
PRIMER_LEFT_2_SELF_END=2.00
4798
PRIMER_RIGHT_2_SELF_END=6.00
4799
PRIMER_LEFT_2_END_STABILITY=11.4000
4800
PRIMER_RIGHT_2_END_STABILITY=7.5000
4801
PRIMER_PAIR_2_COMPL_ANY=5.00
4802
PRIMER_PAIR_2_COMPL_END=2.00
4803
PRIMER_PRODUCT_SIZE_2=140
4804
PRIMER_PAIR_PENALTY_3=0.8098
4805
PRIMER_LEFT_3_PENALTY=0.653497
4806
PRIMER_RIGHT_3_PENALTY=0.156287
4807
PRIMER_LEFT_3_SEQUENCE=CCGCAGCACTGTGTTTATTC
4808
PRIMER_RIGHT_3_SEQUENCE=AAAGTTTGTGTCGAGTGGGC
4809
PRIMER_LEFT_3=223,20
4810
PRIMER_RIGHT_3=368,20
4811
PRIMER_LEFT_3_TM=59.347
4812
PRIMER_RIGHT_3_TM=60.156
4813
PRIMER_LEFT_3_GC_PERCENT=50.000
4814
PRIMER_RIGHT_3_GC_PERCENT=50.000
4815
PRIMER_LEFT_3_SELF_ANY=5.00
4816
PRIMER_RIGHT_3_SELF_ANY=5.00
4817
PRIMER_LEFT_3_SELF_END=0.00
4818
PRIMER_RIGHT_3_SELF_END=2.00
4819
PRIMER_LEFT_3_END_STABILITY=5.9000
4820
PRIMER_RIGHT_3_END_STABILITY=11.2000
4821
PRIMER_PAIR_3_COMPL_ANY=4.00
4822
PRIMER_PAIR_3_COMPL_END=0.00
4823
PRIMER_PRODUCT_SIZE_3=146
4824
PRIMER_PAIR_PENALTY_4=0.8098
4825
PRIMER_LEFT_4_PENALTY=0.653497
4826
PRIMER_RIGHT_4_PENALTY=0.156287
4827
PRIMER_LEFT_4_SEQUENCE=CGCAGCACTGTGTTTATTCC
4828
PRIMER_RIGHT_4_SEQUENCE=AAAGTTTGTGTCGAGTGGGC
4829
PRIMER_LEFT_4=224,20
4830
PRIMER_RIGHT_4=368,20
4831
PRIMER_LEFT_4_TM=59.347
4832
PRIMER_RIGHT_4_TM=60.156
4833
PRIMER_LEFT_4_GC_PERCENT=50.000
4834
PRIMER_RIGHT_4_GC_PERCENT=50.000
4835
PRIMER_LEFT_4_SELF_ANY=5.00
4836
PRIMER_RIGHT_4_SELF_ANY=5.00
4837
PRIMER_LEFT_4_SELF_END=0.00
4838
PRIMER_RIGHT_4_SELF_END=2.00
4839
PRIMER_LEFT_4_END_STABILITY=8.1000
4840
PRIMER_RIGHT_4_END_STABILITY=11.2000
4841
PRIMER_PAIR_4_COMPL_ANY=4.00
4842
PRIMER_PAIR_4_COMPL_END=1.00
4843
PRIMER_PRODUCT_SIZE_4=145
4844
=
4845
MARKER_NAME=46.RHAP33FH10.seq
4846
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNACCTGAGAAATTACCTTGGGTTTGGTAGAACCAGGCAGGAATGAATGATGAAGAAATAAGTGAATAGGTCTTCAGCAGAGGGATTTCTGACCTTCTAGCTATTCATCACTTCTCATAAGTATCTAAGCTTTATAAGTGAGTTTTATCCAGTATGTCATGAGCTCGTGTTTATTTATATGTATTTGGATAAGCGTAAATACAAATGAACACTAATAAAAATACTTGTTAAAAATAGAAATCAAACAAATACACAAAGCAAAGAGATATNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNCGTTTTTATATGANNNCAACCNACCNNGGNAGAAANAANGTNNNNNNNNNNNNNNNNNNNNNNNN
4847
INCLUDED_REGION=26,355
4848
TARGET=293,27,CA
4849
TARGET=321,19,CA
4850
PRIMER_COMMENT=CL is
4851
PRIMER_COMMENT=CR is
4852
=
4853
MARKER_NAME=46.RHAP34FD10.seq
4854
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCTGCTGGATCTGTTTAGTGTTGTTCACGTGCATATGTTTCTAGGCCTAACCACTTACTAGGTATTGGATAATGGATTAGGGCTCAAACCTGGGATGACTGATACTCCTTTTCTCAATAGTTGGATAATGGATTAGGAGCTCAAACCTGGAGAAGACTAATACTCCTTCTCTCAGTAGTTGGATAATAGATTAGGGGCTCAAACCTGGAGAAGACTAATACTCC
4855
INCLUDED_REGION=0,281
4856
TARGET=0,56,CA
4857
PRIMER_COMMENT=CL is
4858
PRIMER_COMMENT=CR is
4859
=
4860
MARKER_NAME=47.RHAP33FD11.seq
4861
SEQUENCE=NNNNNNNNNNNCTGTTTTNGACCTCTTCCTCCTGGAAGGTCAAACTCTAAAGAGGGAACTATAGGGAAGAGTTAGTGGGGCCTTTAAATTGAGCAGCAACCACAAACTCAGTGGGTAGGAAAAAGAGAACAACAAGCTAGCAGGGTCTTTGTTCACCTAGATTCTTTCATATTCTGGGTTTTAAAGAAGTTACTATACATTAACTGTCCTGAAGATGTCCTTAGATAAGTTCCTCGCCCACTTGCCCCACTTCTGTATCCATCCACATTTAAATTCTCTAAAAGGCCATGAATCTTACTTCATTCCATGACTTTTCCCCTAGGGAAATNNNNNNNNNNNNNNNNTGTATGTATGNNNNNNNNNNNNNNNT
4862
INCLUDED_REGION=11,359
4863
TARGET=328,16,TA
4864
TARGET=354,15,TA
4865
PRIMER_COMMENT=CL is
4866
PRIMER_COMMENT=CR is
4867
=
4868
MARKER_NAME=48.RHAP33FD12.seq
4869
SEQUENCE=CNTTTTANGGGGTTGGTGGTTCATCTGAAAGTGTTGAGCACCTTTTCTACTCCTGCTTATATATTTTTATCAAAAGAAGAAGAAGAAGGAGAAGAAAAATGAAATAGCAGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTGANTGATGTGTTTGTACGGNNAGNATGAGNTTTCATNGTTCTCTTNTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTGAAGGATGCTNNTCCTATACANTGAGAGAATATCCTTTATTACNCTGNCNTTAGTTTGTTACATGGAAGAATTTTTGCCTGCNGGTTTAGCCCTCAGGCAAGNCGNG
4870
INCLUDED_REGION=0,361
4871
TARGET=112,58,GT
4872
TARGET=222,28,TCTT
4873
PRIMER_COMMENT=CL is GGGTTGGTGGTTCATCTGAA
4874
PRIMER_COMMENT=CR is GCAGGCAAAAATTCTTCCAT
4875
PRIMER_PAIR_PENALTY=1.5961
4876
PRIMER_LEFT_PENALTY=0.758218
4877
PRIMER_RIGHT_PENALTY=0.837920
4878
PRIMER_LEFT_SEQUENCE=GGGTTGGTGGTTCATCTGAA
4879
PRIMER_RIGHT_SEQUENCE=GCAGGCAAAAATTCTTCCAT
4880
PRIMER_LEFT=9,20
4881
PRIMER_RIGHT=335,20
4882
PRIMER_LEFT_TM=60.758
4883
PRIMER_RIGHT_TM=59.162
4884
PRIMER_LEFT_GC_PERCENT=50.000
4885
PRIMER_RIGHT_GC_PERCENT=40.000
4886
PRIMER_LEFT_SELF_ANY=6.00
4887
PRIMER_RIGHT_SELF_ANY=6.00
4888
PRIMER_LEFT_SELF_END=6.00
4889
PRIMER_RIGHT_SELF_END=6.00
4890
PRIMER_LEFT_END_STABILITY=7.0000
4891
PRIMER_RIGHT_END_STABILITY=8.1000
4892
PRIMER_PAIR_COMPL_ANY=3.00
4893
PRIMER_PAIR_COMPL_END=2.00
4894
PRIMER_PRODUCT_SIZE=327
4895
PRIMER_PAIR_PENALTY_1=1.9365
4896
PRIMER_LEFT_1_PENALTY=0.758218
4897
PRIMER_RIGHT_1_PENALTY=1.178298
4898
PRIMER_LEFT_1_SEQUENCE=GGGTTGGTGGTTCATCTGAA
4899
PRIMER_RIGHT_1_SEQUENCE=CTTGCCTGAGGGCTAAACC
4900
PRIMER_LEFT_1=9,20
4901
PRIMER_RIGHT_1=355,19
4902
PRIMER_LEFT_1_TM=60.758
4903
PRIMER_RIGHT_1_TM=59.822
4904
PRIMER_LEFT_1_GC_PERCENT=50.000
4905
PRIMER_RIGHT_1_GC_PERCENT=57.895
4906
PRIMER_LEFT_1_SELF_ANY=6.00
4907
PRIMER_RIGHT_1_SELF_ANY=5.00
4908
PRIMER_LEFT_1_SELF_END=6.00
4909
PRIMER_RIGHT_1_SELF_END=3.00
4910
PRIMER_LEFT_1_END_STABILITY=7.0000
4911
PRIMER_RIGHT_1_END_STABILITY=8.2000
4912
PRIMER_PAIR_1_COMPL_ANY=4.00
4913
PRIMER_PAIR_1_COMPL_END=0.00
4914
PRIMER_PRODUCT_SIZE_1=347
4915
PRIMER_PAIR_PENALTY_2=2.3988
4916
PRIMER_LEFT_2_PENALTY=1.560893
4917
PRIMER_RIGHT_2_PENALTY=0.837920
4918
PRIMER_LEFT_2_SEQUENCE=GGTTGGTGGTTCATCTGAAAG
4919
PRIMER_RIGHT_2_SEQUENCE=GCAGGCAAAAATTCTTCCAT
4920
PRIMER_LEFT_2=10,21
4921
PRIMER_RIGHT_2=335,20
4922
PRIMER_LEFT_2_TM=59.439
4923
PRIMER_RIGHT_2_TM=59.162
4924
PRIMER_LEFT_2_GC_PERCENT=47.619
4925
PRIMER_RIGHT_2_GC_PERCENT=40.000
4926
PRIMER_LEFT_2_SELF_ANY=6.00
4927
PRIMER_RIGHT_2_SELF_ANY=6.00
4928
PRIMER_LEFT_2_SELF_END=2.00
4929
PRIMER_RIGHT_2_SELF_END=6.00
4930
PRIMER_LEFT_2_END_STABILITY=7.0000
4931
PRIMER_RIGHT_2_END_STABILITY=8.1000
4932
PRIMER_PAIR_2_COMPL_ANY=4.00
4933
PRIMER_PAIR_2_COMPL_END=3.00
4934
PRIMER_PRODUCT_SIZE_2=326
4935
PRIMER_PAIR_PENALTY_3=2.4510
4936
PRIMER_LEFT_3_PENALTY=1.613069
4937
PRIMER_RIGHT_3_PENALTY=0.837920
4938
PRIMER_LEFT_3_SEQUENCE=GGTTGGTGGTTCATCTGAAA
4939
PRIMER_RIGHT_3_SEQUENCE=GCAGGCAAAAATTCTTCCAT
4940
PRIMER_LEFT_3=10,20
4941
PRIMER_RIGHT_3=335,20
4942
PRIMER_LEFT_3_TM=58.387
4943
PRIMER_RIGHT_3_TM=59.162
4944
PRIMER_LEFT_3_GC_PERCENT=45.000
4945
PRIMER_RIGHT_3_GC_PERCENT=40.000
4946
PRIMER_LEFT_3_SELF_ANY=6.00
4947
PRIMER_RIGHT_3_SELF_ANY=6.00
4948
PRIMER_LEFT_3_SELF_END=4.00
4949
PRIMER_RIGHT_3_SELF_END=6.00
4950
PRIMER_LEFT_3_END_STABILITY=7.3000
4951
PRIMER_RIGHT_3_END_STABILITY=8.1000
4952
PRIMER_PAIR_3_COMPL_ANY=3.00
4953
PRIMER_PAIR_3_COMPL_END=2.00
4954
PRIMER_PRODUCT_SIZE_3=326
4955
PRIMER_PAIR_PENALTY_4=2.5356
4956
PRIMER_LEFT_4_PENALTY=1.697647
4957
PRIMER_RIGHT_4_PENALTY=0.837920
4958
PRIMER_LEFT_4_SEQUENCE=GGGTTGGTGGTTCATCTGA
4959
PRIMER_RIGHT_4_SEQUENCE=GCAGGCAAAAATTCTTCCAT
4960
PRIMER_LEFT_4=9,19
4961
PRIMER_RIGHT_4=335,20
4962
PRIMER_LEFT_4_TM=59.302
4963
PRIMER_RIGHT_4_TM=59.162
4964
PRIMER_LEFT_4_GC_PERCENT=52.632
4965
PRIMER_RIGHT_4_GC_PERCENT=40.000
4966
PRIMER_LEFT_4_SELF_ANY=4.00
4967
PRIMER_RIGHT_4_SELF_ANY=6.00
4968
PRIMER_LEFT_4_SELF_END=4.00
4969
PRIMER_RIGHT_4_SELF_END=6.00
4970
PRIMER_LEFT_4_END_STABILITY=6.7000
4971
PRIMER_RIGHT_4_END_STABILITY=8.1000
4972
PRIMER_PAIR_4_COMPL_ANY=3.00
4973
PRIMER_PAIR_4_COMPL_END=1.00
4974
PRIMER_PRODUCT_SIZE_4=327
4975
=
4976
MARKER_NAME=48.RHAP34FD12.seq
4977
SEQUENCE=NNNNNNNNNNACCTAGGTAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATACAACTGTTAACGTAGGAAGCTTCGGTGATCTTCCTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
4978
INCLUDED_REGION=10,120
4979
TARGET=21,33,GT
4980
TARGET=57,32,TATG
4981
PRIMER_COMMENT=CL is
4982
PRIMER_COMMENT=CR is
4983
=
4984
MARKER_NAME=48.RHAP34FH12.seq
4985
SEQUENCE=CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTACAANAAGTCAGGGAGNGGGAGGAGTTCTGNACAGATGGCTTACTGAACCACAATGTGAAAAAGCATGACTTAATGGTGNTCATTTGAGTTTATCCCTTNTCAATACCAACGANAGGGAAGAANTTNAATTGACCAGACACACCGACNNTGAGGGGTCNNAATTGNAACACCNTAGAGAGAGACAAAGAAATGATACTNGNACAAGNTNTGNAAGAGAGNCACTATGTATGNAAGAAGTGNGAGAGNANACATTTCTCCNNGNCACGGTAATAAGCTNGAGAGAGNTNTANTCAACNAAGGAATACATNGGACATAAAAGNGNAAGAAAAGAATCNCATNGTCAACATTGNANTACACAATACACTGNAAGNAGCAAAGNTGA
4986
INCLUDED_REGION=0,420
4987
TARGET=1,34,CA
4988
PRIMER_COMMENT=CL is
4989
PRIMER_COMMENT=CR is
4990
=
4991
MARKER_NAME=03.RHAP61FA3.seq
4992
SEQUENCE=GTCATTGTATTNATCACTACANCAATAAAAANGAAANNGCGACAACTCACAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCGTNTGTATGTCNNTTTANNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNTNGNGTGTGNCCCCTGTATATATGTGCTTNNNNGTGTGANNGCNCGNNTATNNNTNTNNGATNCCNGNTGGNNNNNNNNNNNNNNNNGTCGTNNANNNANNNNNTGNNANNTNAAGGGNNTCNACCCCNANANCANNTCTNTAGGCNCCANNGAANNTNGAAATNNNNNANNNANNGTCATTANCCNGTNNNCTGNCTCCAAANNNNTANCCGNNCAANANNNCCCANAATCANNNCAAGNCNGAANTANNNANCCGCAAANNNNCNGGNNNCNNAANNNTGTCNNNNNNANNCACNNTTANNNCGCNCCCNCNNNTCACCNGCCAGNNTTGCANCNNNGNANAT
4993
INCLUDED_REGION=0,485
4994
TARGET=52,30,GT
4995
TARGET=102,16,GT
4996
TARGET=119,20,TG
4997
TARGET=213,14,TG
4998
PRIMER_COMMENT=CL is
4999
PRIMER_COMMENT=CR is
5000
=
5001
MARKER_NAME=04.RHAP61FB1.seq
5002
SEQUENCE=AGACCCCCTTGTTGATGTGTTTGTGCACAGATCNGTATCTGTGAACACTGACAGAAGGGAGGAAGGAACTGAGTGTCTTGAAAGATATGAGGGAATATGGCTATCTCTGTNTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATNTNNNNTTTTATATACATCTGCATCTTTNCATAGACACCCCATGAAAANGNACCTTGCAAATTCNTTATGGGNNANGTNTGNGTTTGTCAAAATNGGANTNNTCTTGNNAGCTAACTTCCGGNTNNNTAGAATGTATTATCCNCTNTATGTTNGACACTCTNTTGTTGNTGTGTTTCNTAAATAGGGGTANGCGCTCTGNNTTCTGTANNCNTNGGTCNTAGCTGTTTCCTGTTGGNAAATTTNTTNTGCGCNCNTATATTCCNCNNNACATTGTGNGGCGCANNCNTANTGTTTAAACNCNNGGGTCCCNNGTGAGGGCGCGTTTCGCNCNT
5003
INCLUDED_REGION=0,538
5004
TARGET=113,34,AC
5005
TARGET=148,54,GA
5006
PRIMER_COMMENT=CL is
5007
PRIMER_COMMENT=CR is
5008
=
5009
MARKER_NAME=04.RHAP61FB7.seq
5010
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNTGTAGTGTAAGTACCANTTATAACCCTAAGCCAGNTTTGAGAGGCAATAAGTCAACACTTGGATGAGAAAGTGGNACCAGGCCTTTGGTTTGGTCAACAACCTTTTGAGGCAACCATATTATCACCCNTTATTTCACAGATGAAGAAGTTGCACAAAGAAAGTAAAGCAGTATCATATATACACACACATATATGTATACCTACCCATCATATATACATACATATGTTACATATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCGTAAGCTCCNNAGCCNGGCCTGCANNTGGGGGGATCCNNTGNCAGTGCACCGCACANTGTATCACTGCGNGAAGATCCGNCAGGGNNTCTAGTTTCNNCAACNGCNTNNCNGAGGGATGCNGAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
5011
INCLUDED_REGION=21,401
5012
TARGET=257,36,AC
5013
PRIMER_COMMENT=CL is
5014
PRIMER_COMMENT=CR is
5015
=
5016
MARKER_NAME=06.RHAP61FB3.seq
5017
SEQUENCE=TCAAGATGGTGCAGAGGTAAAGGCAGTTGCTGCCAAGATTGAANACCTGACTTCAATCACTNNAACCTACATGTTGGAGGGAANAACGNACTCCAATTTTTCCTCTGACCTTCAACAGGCATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGNNNTGCTTAAATTCAAACANTGANATTGTNATTGGCCAAAATGNTTNATTTCCGCTCACANTTTTANAGCATTCNGGGGTCGGNGGGCNTCCTCCNTNGGNATTTGNNCCNGGGNNANCNACNTTGTCGANAATAAGAACCATGNNCGCGCCAANGANGNNNANNGAGGGTTNCNNGCTCGNNTTNGTANNNNANGGTCANANNNGTTTCNTGGGNNNANNTGGTNTCNGGNNNNNAAATCNNNNCAANNTTCNNGGCCNTGGAGGNATTAAGGNGNNNGCCTTGGGGGGGNNANNGNGTNAGT
5018
INCLUDED_REGION=0,528
5019
TARGET=124,55,AC
5020
TARGET=181,40,TAAA
5021
PRIMER_COMMENT=CL is
5022
PRIMER_COMMENT=CR is
5023
=
5024
MARKER_NAME=06.RHAP61FB9.seq
5025
SEQUENCE=CNTCCNCCNNCCNCCNCTCNCGCCNNNNNNNNNNNNNNNNNNCNNNNNCNNNNNNNNNNNCNNNCNGANTNTGGTGNAATNTGNNGNTCNCNTNGGGGATCNNNAANCNCCCNNANNNNNNNNNNGNNGNNGANNNGTNCNANNTTNNNNNTTGGNGNTNAANNNNNAAATNNNTGNTNNGNAGNANGNGGNNTAAGNNNNCNNTANANGANNNNCNNNANNNNGNNNNNAGTANNNNATNGGNNNNGTTTNGGANGNNGNNATNGNNNAGANNAANNGNGNTGNGACTAGGGNGATNNGGANAAGANGGNNANNGNAAGNGGNGTNNNGGNNTNNTGGNANNTGTNNTGNNNGANNGGNNGNGGNNTAGGANNGNNTGTNNNTNTGGTNNANAAGTNNGNNNTGNNNNTGNNNNNNGGGTTTNNGNANGNNNNNNNNNTTAANNGGNNNNNNANANNTANNTGNNAAAAGGNNNNNNGGGANNNGNNNTAAGNANNGGGNGTNNGGNNGNGGNNNNNTGTGNNNGNNGNTTGGNGNNTNGNAGNNNNGNNNNNNNANGNNNTGNNNAAANT
5026
INCLUDED_REGION=0,572
5027
TARGET=24,16,CN
5028
PRIMER_COMMENT=CL is
5029
PRIMER_COMMENT=CR is
5030
=
5031
MARKER_NAME=07.RHAP61FC7.seq
5032
SEQUENCE=NNNNNNNNNNNTGGGCGTGGTCCCGAGACACTCCCCGCGGGGGCGNCCGGGGCGGGGCGCGGCACCAGGTGGAGGGCGCTGACCCAAGCAACGTCAGTCTGGAAACTGCGTTCCCTAGGCATCTTATCCCCCTAGACCTAGCAGCAGAAGTACCCCAATTCAGTCTCTCAATTCAGTGCTCTTGGAGGAGAGTGTACCCACTCAAGGANTNNCNCGGGCTGGCTGAGCGGGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
5033
INCLUDED_REGION=11,267
5034
TARGET=233,44,AC
5035
PRIMER_COMMENT=CL is
5036
PRIMER_COMMENT=CR is
5037
=
5038
MARKER_NAME=11.RHAP61FD8.seq
5039
SEQUENCE=NNNNNNNNNNNNNNNNNNNTCAGAATTGTTGGGAAGACAGAGTGAAACCCATGGCCTGAGCAGTATCTAGTCTCGGTAANTAGCAAGAAGANAAGGCTAGAAAGGCTGAGGTCTAGATAGGGCTATCCTGGGCTAGACTGAGTGGGTGATCATGACCAACACCAAGGGACTCAGGTGAAGCCCTCTACAAAGGTCCAGACTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGNGNGNGNNNNNNNNNNNNNNNGTCGTGNTGNGGTGTNNGNGTNNGNNNNNNNGGGTGTGNNGTGNNNNNNNGNNTNGGGGNNGNNNNNNNNGGNNNGNNGANNNAGNGGGNGGAGGNCTNTAGNGNANGNNGNNNNNNGGNNANAGNNNNNNNNNGGGTNNNNGNNGNNNANNNNGNNGGNNNANNGTGGGGTNNNGGGGNNAGNNGNNGGNNTNGNGNGAAGNGNNGGGGNNNTGNGNNANNGGGNTNGANNNGNGNGNNNGNGGNA
5040
INCLUDED_REGION=19,483
5041
TARGET=203,28,CT
5042
TARGET=240,15,NC
5043
PRIMER_COMMENT=CL is
5044
PRIMER_COMMENT=CR is
5045
=
5046
MARKER_NAME=12.RHAP61FD9.seq
5047
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCTGTTTTGGAAATTNGAGGAACAAAATATAAAANNCATTAAAAAATTCCCATTTCTGAAAATTATCACTGGAGTTANGGACAATTATGAGCCACACATTGTGGCTCATGATAAGAATNNNCAGAGGGAAANTANGATCTNNNTGNGAAAGAGGGACTNTGNAGNGNCNCNCNNANNGNCNNNAANNNNNCGNTNNGNNNCCNNCTCNNTCACTGNNNNNNNNNNNNNNNNCTNNTCNNTTTNTAGAATCGNGNNGNTGGTCTGAAAACNTNNNNGNNCACNNNNTCTTGGTTANCNTCANTTNTAAAATGNNTGANGNTGNTAGTAGGGNNGCNGCGTGNNNNCCNCCNTCCCAGGNNANCCNGNGCCCCATGNNNCNTCAAANCAANTNNNTGGANCCNCAANGTGNNANGGCCCNNCNATCCNNGNNNNTCNTGNNNNNGNGNTCATTGCAAANTNAGNGNNNNNNNNCNNNANTG
5048
INCLUDED_REGION=21,478
5049
TARGET=236,14,CT
5050
PRIMER_COMMENT=CL is
5051
PRIMER_COMMENT=CR is
5052
=
5053
MARKER_NAME=13.RHAP61FE7.seq
5054
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCTTGAACATTTACTACATTTCCTGGGCTCACCANTTTGTNAATTTGCCTTTTTCTGTTCCATATGCANTTTTACCATCATCAGGTATAATGAATGGGTCTTAGCAGATTCCACTTGGGTGNTCTANTGAATTCTNNNNNNNNNNNNNNNNNNNNNNNNNGNGNNNCCNCCCNCNNNNNCNNTGNNNNNNNNNNNTGNNNNNGNCNCNNNNNCGGNNNGNGGGNNGCTGNGGAANNNNGGNNGNGNNGGGGNGNNNNGGGGNNNGNNNNGGGTGGGGGNGNNGCAGTGNNNNGGGGNNGNGGNGNGNNTGNGGNGTGGNNGNNNGGNNGTGNNNGGGNGGNGGGGGTTGGGNGNNGNNGTNNNAANNGGGGGNNNNGTNGGGGGNNGNNNNGNNNNNNNNNGGGANNNGGGGGNNTTNNNNGGGNGAGNNNGANNNGNNNNNGGGNNNANNNNNGAGGGNNGGNANNNGTGNGGGNNAGGGNNNGTTNNGGNNTTAAA
5055
INCLUDED_REGION=21,497
5056
TARGET=155,24,TC
5057
PRIMER_COMMENT=CL is
5058
PRIMER_COMMENT=CR is
5059
=
5060
MARKER_NAME=14.RHAP61FE8.seq
5061
SEQUENCE=NNNNNNNNNNNNNNNNNNNTAGGACTCTGAAGTATATTCAGGTACTTGTGATGCTGAGGGAGCCTGAAGGCAGCACCGAGTTTTGGTGTGCTGATAGAAACCAAAGATAGTTATTTGTCCCTTCTGCCTTTTAGGATCTGGGGAAACGGAGTTTCTTTTGGACCTGACAGCAGTTTGCATGNGNGTCNGNGATGTATTTTGATCACGTTCACCGTTATCTTGTCCCCCTCCCACCCCTACAGACCTTCTACCTCTGTGTGTGCATGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCANTGGTGTTACAGGAAAATGAGAGAGGTTCGTTACACTGGGGCACCTTGCCAGTGGCGTACATCACTGACAAAGTTTCTNTCCCTCCCAGCAAATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
5062
INCLUDED_REGION=19,401
5063
TARGET=268,50,TG
5064
PRIMER_COMMENT=CL is ACCGTTATCTTGTCCCCCTC
5065
PRIMER_COMMENT=CR is CCCCAGTGTAACGAACCTCT
5066
PRIMER_PAIR_PENALTY=1.1286
5067
PRIMER_LEFT_PENALTY=0.190281
5068
PRIMER_RIGHT_PENALTY=0.938316
5069
PRIMER_LEFT_SEQUENCE=ACCGTTATCTTGTCCCCCTC
5070
PRIMER_RIGHT_SEQUENCE=CCCCAGTGTAACGAACCTCT
5071
PRIMER_LEFT=210,20
5072
PRIMER_RIGHT=362,20
5073
PRIMER_LEFT_TM=60.190
5074
PRIMER_RIGHT_TM=59.062
5075
PRIMER_LEFT_GC_PERCENT=55.000
5076
PRIMER_RIGHT_GC_PERCENT=55.000
5077
PRIMER_LEFT_SELF_ANY=3.00
5078
PRIMER_RIGHT_SELF_ANY=3.00
5079
PRIMER_LEFT_SELF_END=0.00
5080
PRIMER_RIGHT_SELF_END=0.00
5081
PRIMER_LEFT_END_STABILITY=9.4000
5082
PRIMER_RIGHT_END_STABILITY=7.9000
5083
PRIMER_PAIR_COMPL_ANY=5.00
5084
PRIMER_PAIR_COMPL_END=0.00
5085
PRIMER_PRODUCT_SIZE=153
5086
PRIMER_PAIR_PENALTY_1=1.1480
5087
PRIMER_LEFT_1_PENALTY=0.209659
5088
PRIMER_RIGHT_1_PENALTY=0.938316
5089
PRIMER_LEFT_1_SEQUENCE=TTCACCGTTATCTTGTCCCC
5090
PRIMER_RIGHT_1_SEQUENCE=CCCCAGTGTAACGAACCTCT
5091
PRIMER_LEFT_1=207,20
5092
PRIMER_RIGHT_1=362,20
5093
PRIMER_LEFT_1_TM=59.790
5094
PRIMER_RIGHT_1_TM=59.062
5095
PRIMER_LEFT_1_GC_PERCENT=50.000
5096
PRIMER_RIGHT_1_GC_PERCENT=55.000
5097
PRIMER_LEFT_1_SELF_ANY=3.00
5098
PRIMER_RIGHT_1_SELF_ANY=3.00
5099
PRIMER_LEFT_1_SELF_END=0.00
5100
PRIMER_RIGHT_1_SELF_END=0.00
5101
PRIMER_LEFT_1_END_STABILITY=10.9000
5102
PRIMER_RIGHT_1_END_STABILITY=7.9000
5103
PRIMER_PAIR_1_COMPL_ANY=5.00
5104
PRIMER_PAIR_1_COMPL_END=0.00
5105
PRIMER_PRODUCT_SIZE_1=156
5106
PRIMER_PAIR_PENALTY_2=1.2088
5107
PRIMER_LEFT_2_PENALTY=0.190281
5108
PRIMER_RIGHT_2_PENALTY=1.018512
5109
PRIMER_LEFT_2_SEQUENCE=ACCGTTATCTTGTCCCCCTC
5110
PRIMER_RIGHT_2_SEQUENCE=GCCCCAGTGTAACGAACCT
5111
PRIMER_LEFT_2=210,20
5112
PRIMER_RIGHT_2=363,19
5113
PRIMER_LEFT_2_TM=60.190
5114
PRIMER_RIGHT_2_TM=59.981
5115
PRIMER_LEFT_2_GC_PERCENT=55.000
5116
PRIMER_RIGHT_2_GC_PERCENT=57.895
5117
PRIMER_LEFT_2_SELF_ANY=3.00
5118
PRIMER_RIGHT_2_SELF_ANY=3.00
5119
PRIMER_LEFT_2_SELF_END=0.00
5120
PRIMER_RIGHT_2_SELF_END=0.00
5121
PRIMER_LEFT_2_END_STABILITY=9.4000
5122
PRIMER_RIGHT_2_END_STABILITY=7.9000
5123
PRIMER_PAIR_2_COMPL_ANY=5.00
5124
PRIMER_PAIR_2_COMPL_END=0.00
5125
PRIMER_PRODUCT_SIZE_2=154
5126
PRIMER_PAIR_PENALTY_3=1.2282
5127
PRIMER_LEFT_3_PENALTY=0.209659
5128
PRIMER_RIGHT_3_PENALTY=1.018512
5129
PRIMER_LEFT_3_SEQUENCE=TTCACCGTTATCTTGTCCCC
5130
PRIMER_RIGHT_3_SEQUENCE=GCCCCAGTGTAACGAACCT
5131
PRIMER_LEFT_3=207,20
5132
PRIMER_RIGHT_3=363,19
5133
PRIMER_LEFT_3_TM=59.790
5134
PRIMER_RIGHT_3_TM=59.981
5135
PRIMER_LEFT_3_GC_PERCENT=50.000
5136
PRIMER_RIGHT_3_GC_PERCENT=57.895
5137
PRIMER_LEFT_3_SELF_ANY=3.00
5138
PRIMER_RIGHT_3_SELF_ANY=3.00
5139
PRIMER_LEFT_3_SELF_END=0.00
5140
PRIMER_RIGHT_3_SELF_END=0.00
5141
PRIMER_LEFT_3_END_STABILITY=10.9000
5142
PRIMER_RIGHT_3_END_STABILITY=7.9000
5143
PRIMER_PAIR_3_COMPL_ANY=5.00
5144
PRIMER_PAIR_3_COMPL_END=0.00
5145
PRIMER_PRODUCT_SIZE_3=157
5146
PRIMER_PAIR_PENALTY_4=1.3009
5147
PRIMER_LEFT_4_PENALTY=0.362536
5148
PRIMER_RIGHT_4_PENALTY=0.938316
5149
PRIMER_LEFT_4_SEQUENCE=CTCCCACCCCTACAGACCTT
5150
PRIMER_RIGHT_4_SEQUENCE=CCCCAGTGTAACGAACCTCT
5151
PRIMER_LEFT_4=227,20
5152
PRIMER_RIGHT_4=362,20
5153
PRIMER_LEFT_4_TM=60.363
5154
PRIMER_RIGHT_4_TM=59.062
5155
PRIMER_LEFT_4_GC_PERCENT=60.000
5156
PRIMER_RIGHT_4_GC_PERCENT=55.000
5157
PRIMER_LEFT_4_SELF_ANY=2.00
5158
PRIMER_RIGHT_4_SELF_ANY=3.00
5159
PRIMER_LEFT_4_SELF_END=0.00
5160
PRIMER_RIGHT_4_SELF_END=0.00
5161
PRIMER_LEFT_4_END_STABILITY=7.9000
5162
PRIMER_RIGHT_4_END_STABILITY=7.9000
5163
PRIMER_PAIR_4_COMPL_ANY=4.00
5164
PRIMER_PAIR_4_COMPL_END=3.00
5165
PRIMER_PRODUCT_SIZE_4=136
5166
=
5167
MARKER_NAME=16.RHAP61FF1.seq
5168
SEQUENCE=NNNNNNNNNNNTGTCATTTTCTTTTTAATCTCTGTTTTTCTGCCATTCTAGGTCAGCTGAAGATGAAGGTCTCCTCTACTGCTCTGGAGCCTNTNCACGGAGACCCTAATTTCATGCATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGTTCCNTTGAAATAATGGAAGATATTAAAGTATAAAAATAAGAGAGTTGTACCATCCATACCCATTCTGAAAATGAAGCCAAATGTAATCTTATCACTAAAGAGCATGTTATATNTGAACAGAGACATTTTATACACTATAAGATTAGATCTCAAANTNNTTATNTACCTGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
5169
INCLUDED_REGION=11,327
5170
TARGET=120,44,CA
5171
PRIMER_COMMENT=CL is TCCTCTACTGCTCTGGAGCC
5172
PRIMER_COMMENT=CR is GGCTTCATTTTCAGAATGGG
5173
PRIMER_PAIR_PENALTY=0.7921
5174
PRIMER_LEFT_PENALTY=0.296722
5175
PRIMER_RIGHT_PENALTY=0.495347
5176
PRIMER_LEFT_SEQUENCE=TCCTCTACTGCTCTGGAGCC
5177
PRIMER_RIGHT_SEQUENCE=GGCTTCATTTTCAGAATGGG
5178
PRIMER_LEFT=71,20
5179
PRIMER_RIGHT=244,20
5180
PRIMER_LEFT_TM=59.703
5181
PRIMER_RIGHT_TM=59.505
5182
PRIMER_LEFT_GC_PERCENT=60.000
5183
PRIMER_RIGHT_GC_PERCENT=45.000
5184
PRIMER_LEFT_SELF_ANY=6.00
5185
PRIMER_RIGHT_SELF_ANY=7.00
5186
PRIMER_LEFT_SELF_END=4.00
5187
PRIMER_RIGHT_SELF_END=3.00
5188
PRIMER_LEFT_END_STABILITY=9.4000
5189
PRIMER_RIGHT_END_STABILITY=9.6000
5190
PRIMER_PAIR_COMPL_ANY=5.00
5191
PRIMER_PAIR_COMPL_END=3.00
5192
PRIMER_PRODUCT_SIZE=174
5193
PRIMER_PAIR_PENALTY_1=0.9674
5194
PRIMER_LEFT_1_PENALTY=0.472052
5195
PRIMER_RIGHT_1_PENALTY=0.495347
5196
PRIMER_LEFT_1_SEQUENCE=CGGAGACCCTAATTTCATGC
5197
PRIMER_RIGHT_1_SEQUENCE=GGCTTCATTTTCAGAATGGG
5198
PRIMER_LEFT_1=97,20
5199
PRIMER_RIGHT_1=244,20
5200
PRIMER_LEFT_1_TM=59.528
5201
PRIMER_RIGHT_1_TM=59.505
5202
PRIMER_LEFT_1_GC_PERCENT=50.000
5203
PRIMER_RIGHT_1_GC_PERCENT=45.000
5204
PRIMER_LEFT_1_SELF_ANY=4.00
5205
PRIMER_RIGHT_1_SELF_ANY=7.00
5206
PRIMER_LEFT_1_SELF_END=2.00
5207
PRIMER_RIGHT_1_SELF_END=3.00
5208
PRIMER_LEFT_1_END_STABILITY=8.4000
5209
PRIMER_RIGHT_1_END_STABILITY=9.6000
5210
PRIMER_PAIR_1_COMPL_ANY=3.00
5211
PRIMER_PAIR_1_COMPL_END=1.00
5212
PRIMER_PRODUCT_SIZE_1=148
5213
PRIMER_PAIR_PENALTY_2=1.5320
5214
PRIMER_LEFT_2_PENALTY=0.472052
5215
PRIMER_RIGHT_2_PENALTY=1.059980
5216
PRIMER_LEFT_2_SEQUENCE=CGGAGACCCTAATTTCATGC
5217
PRIMER_RIGHT_2_SEQUENCE=TTGGCTTCATTTTCAGAATGG
5218
PRIMER_LEFT_2=97,20
5219
PRIMER_RIGHT_2=246,21
5220
PRIMER_LEFT_2_TM=59.528
5221
PRIMER_RIGHT_2_TM=60.060
5222
PRIMER_LEFT_2_GC_PERCENT=50.000
5223
PRIMER_RIGHT_2_GC_PERCENT=38.095
5224
PRIMER_LEFT_2_SELF_ANY=4.00
5225
PRIMER_RIGHT_2_SELF_ANY=7.00
5226
PRIMER_LEFT_2_SELF_END=2.00
5227
PRIMER_RIGHT_2_SELF_END=5.00
5228
PRIMER_LEFT_2_END_STABILITY=8.4000
5229
PRIMER_RIGHT_2_END_STABILITY=8.4000
5230
PRIMER_PAIR_2_COMPL_ANY=3.00
5231
PRIMER_PAIR_2_COMPL_END=2.00
5232
PRIMER_PRODUCT_SIZE_2=150
5233
PRIMER_PAIR_PENALTY_3=1.6016
5234
PRIMER_LEFT_3_PENALTY=0.296722
5235
PRIMER_RIGHT_3_PENALTY=1.304835
5236
PRIMER_LEFT_3_SEQUENCE=TCCTCTACTGCTCTGGAGCC
5237
PRIMER_RIGHT_3_SEQUENCE=TGGCTTCATTTTCAGAATGG
5238
PRIMER_LEFT_3=71,20
5239
PRIMER_RIGHT_3=245,20
5240
PRIMER_LEFT_3_TM=59.703
5241
PRIMER_RIGHT_3_TM=58.695
5242
PRIMER_LEFT_3_GC_PERCENT=60.000
5243
PRIMER_RIGHT_3_GC_PERCENT=40.000
5244
PRIMER_LEFT_3_SELF_ANY=6.00
5245
PRIMER_RIGHT_3_SELF_ANY=7.00
5246
PRIMER_LEFT_3_SELF_END=4.00
5247
PRIMER_RIGHT_3_SELF_END=5.00
5248
PRIMER_LEFT_3_END_STABILITY=9.4000
5249
PRIMER_RIGHT_3_END_STABILITY=8.4000
5250
PRIMER_PAIR_3_COMPL_ANY=5.00
5251
PRIMER_PAIR_3_COMPL_END=2.00
5252
PRIMER_PRODUCT_SIZE_3=175
5253
PRIMER_PAIR_PENALTY_4=1.6100
5254
PRIMER_LEFT_4_PENALTY=0.296722
5255
PRIMER_RIGHT_4_PENALTY=1.313269
5256
PRIMER_LEFT_4_SEQUENCE=TCCTCTACTGCTCTGGAGCC
5257
PRIMER_RIGHT_4_SEQUENCE=GGCTTCATTTTCAGAATGGGT
5258
PRIMER_LEFT_4=71,20
5259
PRIMER_RIGHT_4=244,21
5260
PRIMER_LEFT_4_TM=59.703
5261
PRIMER_RIGHT_4_TM=60.313
5262
PRIMER_LEFT_4_GC_PERCENT=60.000
5263
PRIMER_RIGHT_4_GC_PERCENT=42.857
5264
PRIMER_LEFT_4_SELF_ANY=6.00
5265
PRIMER_RIGHT_4_SELF_ANY=7.00
5266
PRIMER_LEFT_4_SELF_END=4.00
5267
PRIMER_RIGHT_4_SELF_END=1.00
5268
PRIMER_LEFT_4_END_STABILITY=9.4000
5269
PRIMER_RIGHT_4_END_STABILITY=9.4000
5270
PRIMER_PAIR_4_COMPL_ANY=5.00
5271
PRIMER_PAIR_4_COMPL_END=2.00
5272
PRIMER_PRODUCT_SIZE_4=174
5273
=
5274
MARKER_NAME=18.RHAP61FF3.seq
5275
SEQUENCE=GTAGCCGACACCATCCCCCGACACATACATACATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAACCAAACAAACAAACAAACAAATCAGCAAAACGTTTAGTCAATTCTGCTTTTCTTACTGGAAAGCCCAAAGCCTGTGTCTTAGCATGGCTGATTATCCTCTCTTCTGGGCAAGATTTCCTCAGTGCCAGTTTTATGTTTGCAAGGTCCCATCTATGCCAAGCATCATGTTAGCCCTAATATTTCTGATGCATTCCAAGCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
5276
INCLUDED_REGION=0,271
5277
TARGET=34,34,AC
5278
PRIMER_COMMENT=CL is TCCCCCGACACATACATACA
5279
PRIMER_COMMENT=CR is TAAGACACAGGCTTTGGGCT
5280
PRIMER_PAIR_PENALTY=0.4765
5281
PRIMER_LEFT_PENALTY=0.351810
5282
PRIMER_RIGHT_PENALTY=0.124655
5283
PRIMER_LEFT_SEQUENCE=TCCCCCGACACATACATACA
5284
PRIMER_RIGHT_SEQUENCE=GCTAAGACACAGGCTTTGGG
5285
PRIMER_LEFT=13,20
5286
PRIMER_RIGHT=153,20
5287
PRIMER_LEFT_TM=59.648
5288
PRIMER_RIGHT_TM=59.875
5289
PRIMER_LEFT_GC_PERCENT=50.000
5290
PRIMER_RIGHT_GC_PERCENT=55.000
5291
PRIMER_LEFT_SELF_ANY=2.00
5292
PRIMER_RIGHT_SELF_ANY=5.00
5293
PRIMER_LEFT_SELF_END=0.00
5294
PRIMER_RIGHT_SELF_END=1.00
5295
PRIMER_LEFT_END_STABILITY=5.6000
5296
PRIMER_RIGHT_END_STABILITY=10.0000
5297
PRIMER_PAIR_COMPL_ANY=3.00
5298
PRIMER_PAIR_COMPL_END=0.00
5299
PRIMER_PRODUCT_SIZE=141
5300
PRIMER_PAIR_PENALTY_1=0.4765
5301
PRIMER_LEFT_1_PENALTY=0.351810
5302
PRIMER_RIGHT_1_PENALTY=0.124655
5303
PRIMER_LEFT_1_SEQUENCE=TCCCCCGACACATACATACA
5304
PRIMER_RIGHT_1_SEQUENCE=CTAAGACACAGGCTTTGGGC
5305
PRIMER_LEFT_1=13,20
5306
PRIMER_RIGHT_1=152,20
5307
PRIMER_LEFT_1_TM=59.648
5308
PRIMER_RIGHT_1_TM=59.875
5309
PRIMER_LEFT_1_GC_PERCENT=50.000
5310
PRIMER_RIGHT_1_GC_PERCENT=55.000
5311
PRIMER_LEFT_1_SELF_ANY=2.00
5312
PRIMER_RIGHT_1_SELF_ANY=5.00
5313
PRIMER_LEFT_1_SELF_END=0.00
5314
PRIMER_RIGHT_1_SELF_END=2.00
5315
PRIMER_LEFT_1_END_STABILITY=5.6000
5316
PRIMER_RIGHT_1_END_STABILITY=11.2000
5317
PRIMER_PAIR_1_COMPL_ANY=3.00
5318
PRIMER_PAIR_1_COMPL_END=0.00
5319
PRIMER_PRODUCT_SIZE_1=140
5320
PRIMER_PAIR_PENALTY_2=0.4765
5321
PRIMER_LEFT_2_PENALTY=0.351810
5322
PRIMER_RIGHT_2_PENALTY=0.124655
5323
PRIMER_LEFT_2_SEQUENCE=TCCCCCGACACATACATACA
5324
PRIMER_RIGHT_2_SEQUENCE=TAAGACACAGGCTTTGGGCT
5325
PRIMER_LEFT_2=13,20
5326
PRIMER_RIGHT_2=151,20
5327
PRIMER_LEFT_2_TM=59.648
5328
PRIMER_RIGHT_2_TM=59.875
5329
PRIMER_LEFT_2_GC_PERCENT=50.000
5330
PRIMER_RIGHT_2_GC_PERCENT=50.000
5331
PRIMER_LEFT_2_SELF_ANY=2.00
5332
PRIMER_RIGHT_2_SELF_ANY=5.00
5333
PRIMER_LEFT_2_SELF_END=0.00
5334
PRIMER_RIGHT_2_SELF_END=3.00
5335
PRIMER_LEFT_2_END_STABILITY=5.6000
5336
PRIMER_RIGHT_2_END_STABILITY=10.9000
5337
PRIMER_PAIR_2_COMPL_ANY=3.00
5338
PRIMER_PAIR_2_COMPL_END=1.00
5339
PRIMER_PRODUCT_SIZE_2=139
5340
PRIMER_PAIR_PENALTY_3=0.7793
5341
PRIMER_LEFT_3_PENALTY=0.351810
5342
PRIMER_RIGHT_3_PENALTY=0.427522
5343
PRIMER_LEFT_3_SEQUENCE=TCCCCCGACACATACATACA
5344
PRIMER_RIGHT_3_SEQUENCE=AGCCATGCTAAGACACAGGC
5345
PRIMER_LEFT_3=13,20
5346
PRIMER_RIGHT_3=159,20
5347
PRIMER_LEFT_3_TM=59.648
5348
PRIMER_RIGHT_3_TM=60.428
5349
PRIMER_LEFT_3_GC_PERCENT=50.000
5350
PRIMER_RIGHT_3_GC_PERCENT=55.000
5351
PRIMER_LEFT_3_SELF_ANY=2.00
5352
PRIMER_RIGHT_3_SELF_ANY=4.00
5353
PRIMER_LEFT_3_SELF_END=0.00
5354
PRIMER_RIGHT_3_SELF_END=3.00
5355
PRIMER_LEFT_3_END_STABILITY=5.6000
5356
PRIMER_RIGHT_3_END_STABILITY=9.7000
5357
PRIMER_PAIR_3_COMPL_ANY=3.00
5358
PRIMER_PAIR_3_COMPL_END=0.00
5359
PRIMER_PRODUCT_SIZE_3=147
5360
PRIMER_PAIR_PENALTY_4=0.7793
5361
PRIMER_LEFT_4_PENALTY=0.351810
5362
PRIMER_RIGHT_4_PENALTY=0.427522
5363
PRIMER_LEFT_4_SEQUENCE=TCCCCCGACACATACATACA
5364
PRIMER_RIGHT_4_SEQUENCE=GCCATGCTAAGACACAGGCT
5365
PRIMER_LEFT_4=13,20
5366
PRIMER_RIGHT_4=158,20
5367
PRIMER_LEFT_4_TM=59.648
5368
PRIMER_RIGHT_4_TM=60.428
5369
PRIMER_LEFT_4_GC_PERCENT=50.000
5370
PRIMER_RIGHT_4_GC_PERCENT=55.000
5371
PRIMER_LEFT_4_SELF_ANY=2.00
5372
PRIMER_RIGHT_4_SELF_ANY=4.00
5373
PRIMER_LEFT_4_SELF_END=0.00
5374
PRIMER_RIGHT_4_SELF_END=3.00
5375
PRIMER_LEFT_4_END_STABILITY=5.6000
5376
PRIMER_RIGHT_4_END_STABILITY=9.4000
5377
PRIMER_PAIR_4_COMPL_ANY=3.00
5378
PRIMER_PAIR_4_COMPL_END=1.00
5379
PRIMER_PRODUCT_SIZE_4=146
5380
=
5381
MARKER_NAME=19.RHAP61FG1.seq
5382
SEQUENCE=NNACNNACTTANTCCNNAAAGNANNACNNNNNNNNNNNNNNNNTGNCGNNNNNNNNNNNNNNNNNNNACCCATNNAGNCATACATACNGTCACATGTGTGTATANACANCNGNAGTCAACCNNGCNTCTTNTTCCNCAATTANANCNCCTNAANANNANNGNNNNNTGATAGGNCNATTACNTCNCCACTNACCTGNGTNNNNGCGGNCTCTNNANTTCGGGGNATCATTNNCTTGAACGGCNCNCCNNTGNTNAANNNTTTGGCNGNNGGNNGNTTNNCACNNANGATGNGTNNNNNGGNNNGCATNNNAGNTGTNAAGGCNTCGGGNGGGNNNGTATGGGAGGTGNGGNNNGTCNANNGGANGGNNTCGNNGGNGNNCANGTTCCNGNNGNNNGNNNNCNNGAANTNTTNNNGNNNNGGNNGGNGNGANGNNAGGATGNNAGNTCNNNNNGANNGTGNGNTTNGNNGNNTANGNGNNNCTGGGGGGGGNANAANNTTNNNGGGTGGTGTCGNTGGNNTGGNTGGTTTGNNTGCNTAGNNT
5383
INCLUDED_REGION=0,542
5384
TARGET=29,14,NG
5385
TARGET=49,18,GT
5386
PRIMER_COMMENT=CL is
5387
PRIMER_COMMENT=CR is
5388
=
5389
MARKER_NAME=20.RHAP61FG2.seq
5390
SEQUENCE=NNNNNNNNNNNNTAACAACTGAGACAGAAGAAAATGTCAGAAATGAAAAAAATAAAAAAAGCAGAAAACTNACTNATCAACCGTAAAAACAAACCAACCAAAATGTAAATTAAAAATCTAAAACACTTATACGTGACATTCAGAAATGTCTGTCAATGACTGAGAATTATTCACATCTAAACAATGAAAGAACACACAGTGCAATTATATCTGTATTAAATGTTTCCAGTTTGGGAGATATGCATTGAAGTACCAATATTGATCAAGATCTTTCTTGCGGGTGTGTGAGTATTAAATATGAATGTGNNAACAGTATGTTAACATCTGAGAGCATNCTCCATCATTCTGTCACCTCAGGTTTCATAGAAGAAATNTCCTATTGNCATGTTGTAGACTNTTTGGGAAAGNGNTANNNGCACTTTNAATCCCAGNNTCACCCTCTCAGGTCNTGGGATCTGNNCCACNGCCGTCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCATGAAGT
5391
INCLUDED_REGION=12,509
5392
TARGET=475,37,GT
5393
PRIMER_COMMENT=CL is
5394
PRIMER_COMMENT=CR is
5395
=
5396
MARKER_NAME=21.RHAP61FG9.seq
5397
SEQUENCE=AATGANTTAGAGGACCCCTTCCTCACAGGTCCAGAAGGACACTGAAGAANAGGTTAGCCAGATATGCTAAGGCTCTTGTCACTAGTCAGTTCTGAATTGCTTCCCTTTAATGTCTTGTCCGGGAAGGGACTGAGAATCCANTCANACATGTCTACCNTGNGNCTCNGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTNTCTCTCTCCTCAACCCAACCCAAACCACATTTCCCCCCCACACNNTCCCTCATATCNCCTTGNTTGNNATTCCCCCCCCCCCCCNACTNANANNTACNCNTNNNTTNTTTNAANNANAAAACCNCTNNNAAAAAAAACCNNNNGTAGNNNNNANANCNAAAATTTNTAAANANGNNNNNNG
5398
INCLUDED_REGION=0,479
5399
TARGET=169,44,TG
5400
TARGET=215,40,AG
5401
TARGET=262,30,GA
5402
PRIMER_COMMENT=CL is TAATGTCTTGTCCGGGAAGG
5403
PRIMER_COMMENT=CR is GGTTGGGTTGAGGAGAGAGA
5404
PRIMER_PAIR_PENALTY=0.8463
5405
PRIMER_LEFT_PENALTY=0.074208
5406
PRIMER_RIGHT_PENALTY=0.772097
5407
PRIMER_LEFT_SEQUENCE=TAATGTCTTGTCCGGGAAGG
5408
PRIMER_RIGHT_SEQUENCE=GGTTGGGTTGAGGAGAGAGA
5409
PRIMER_LEFT=107,20
5410
PRIMER_RIGHT=317,20
5411
PRIMER_LEFT_TM=59.926
5412
PRIMER_RIGHT_TM=59.228
5413
PRIMER_LEFT_GC_PERCENT=50.000
5414
PRIMER_RIGHT_GC_PERCENT=55.000
5415
PRIMER_LEFT_SELF_ANY=5.00
5416
PRIMER_RIGHT_SELF_ANY=1.00
5417
PRIMER_LEFT_SELF_END=3.00
5418
PRIMER_RIGHT_SELF_END=0.00
5419
PRIMER_LEFT_END_STABILITY=8.2000
5420
PRIMER_RIGHT_END_STABILITY=6.4000
5421
PRIMER_PAIR_COMPL_ANY=4.00
5422
PRIMER_PAIR_COMPL_END=0.00
5423
PRIMER_PRODUCT_SIZE=211
5424
PRIMER_PAIR_PENALTY_1=1.0013
5425
PRIMER_LEFT_1_PENALTY=0.229181
5426
PRIMER_RIGHT_1_PENALTY=0.772097
5427
PRIMER_LEFT_1_SEQUENCE=TTCCTCACAGGTCCAGAAGG
5428
PRIMER_RIGHT_1_SEQUENCE=GGTTGGGTTGAGGAGAGAGA
5429
PRIMER_LEFT_1=18,20
5430
PRIMER_RIGHT_1=317,20
5431
PRIMER_LEFT_1_TM=60.229
5432
PRIMER_RIGHT_1_TM=59.228
5433
PRIMER_LEFT_1_GC_PERCENT=55.000
5434
PRIMER_RIGHT_1_GC_PERCENT=55.000
5435
PRIMER_LEFT_1_SELF_ANY=3.00
5436
PRIMER_RIGHT_1_SELF_ANY=1.00
5437
PRIMER_LEFT_1_SELF_END=3.00
5438
PRIMER_RIGHT_1_SELF_END=0.00
5439
PRIMER_LEFT_1_END_STABILITY=8.2000
5440
PRIMER_RIGHT_1_END_STABILITY=6.4000
5441
PRIMER_PAIR_1_COMPL_ANY=6.00
5442
PRIMER_PAIR_1_COMPL_END=0.00
5443
PRIMER_PRODUCT_SIZE_1=300
5444
PRIMER_PAIR_PENALTY_2=1.0013
5445
PRIMER_LEFT_2_PENALTY=0.229181
5446
PRIMER_RIGHT_2_PENALTY=0.772097
5447
PRIMER_LEFT_2_SEQUENCE=CCTTCCTCACAGGTCCAGAA
5448
PRIMER_RIGHT_2_SEQUENCE=GGTTGGGTTGAGGAGAGAGA
5449
PRIMER_LEFT_2=16,20
5450
PRIMER_RIGHT_2=317,20
5451
PRIMER_LEFT_2_TM=60.229
5452
PRIMER_RIGHT_2_TM=59.228
5453
PRIMER_LEFT_2_GC_PERCENT=55.000
5454
PRIMER_RIGHT_2_GC_PERCENT=55.000
5455
PRIMER_LEFT_2_SELF_ANY=3.00
5456
PRIMER_RIGHT_2_SELF_ANY=1.00
5457
PRIMER_LEFT_2_SELF_END=2.00
5458
PRIMER_RIGHT_2_SELF_END=0.00
5459
PRIMER_LEFT_2_END_STABILITY=7.0000
5460
PRIMER_RIGHT_2_END_STABILITY=6.4000
5461
PRIMER_PAIR_2_COMPL_ANY=6.00
5462
PRIMER_PAIR_2_COMPL_END=1.00
5463
PRIMER_PRODUCT_SIZE_2=302
5464
PRIMER_PAIR_PENALTY_3=1.0900
5465
PRIMER_LEFT_3_PENALTY=0.317911
5466
PRIMER_RIGHT_3_PENALTY=0.772097
5467
PRIMER_LEFT_3_SEQUENCE=CCTCACAGGTCCAGAAGGAC
5468
PRIMER_RIGHT_3_SEQUENCE=GGTTGGGTTGAGGAGAGAGA
5469
PRIMER_LEFT_3=20,20
5470
PRIMER_RIGHT_3=317,20
5471
PRIMER_LEFT_3_TM=59.682
5472
PRIMER_RIGHT_3_TM=59.228
5473
PRIMER_LEFT_3_GC_PERCENT=60.000
5474
PRIMER_RIGHT_3_GC_PERCENT=55.000
5475
PRIMER_LEFT_3_SELF_ANY=4.00
5476
PRIMER_RIGHT_3_SELF_ANY=1.00
5477
PRIMER_LEFT_3_SELF_END=4.00
5478
PRIMER_RIGHT_3_SELF_END=0.00
5479
PRIMER_LEFT_3_END_STABILITY=7.6000
5480
PRIMER_RIGHT_3_END_STABILITY=6.4000
5481
PRIMER_PAIR_3_COMPL_ANY=5.00
5482
PRIMER_PAIR_3_COMPL_END=0.00
5483
PRIMER_PRODUCT_SIZE_3=298
5484
PRIMER_PAIR_PENALTY_4=1.0949
5485
PRIMER_LEFT_4_PENALTY=0.074208
5486
PRIMER_RIGHT_4_PENALTY=1.020670
5487
PRIMER_LEFT_4_SEQUENCE=TAATGTCTTGTCCGGGAAGG
5488
PRIMER_RIGHT_4_SEQUENCE=GGGTTGGGTTGAGGAGAGAG
5489
PRIMER_LEFT_4=107,20
5490
PRIMER_RIGHT_4=318,20
5491
PRIMER_LEFT_4_TM=59.926
5492
PRIMER_RIGHT_4_TM=61.021
5493
PRIMER_LEFT_4_GC_PERCENT=50.000
5494
PRIMER_RIGHT_4_GC_PERCENT=60.000
5495
PRIMER_LEFT_4_SELF_ANY=5.00
5496
PRIMER_RIGHT_4_SELF_ANY=1.00
5497
PRIMER_LEFT_4_SELF_END=3.00
5498
PRIMER_RIGHT_4_SELF_END=0.00
5499
PRIMER_LEFT_4_END_STABILITY=8.2000
5500
PRIMER_RIGHT_4_END_STABILITY=6.4000
5501
PRIMER_PAIR_4_COMPL_ANY=3.00
5502
PRIMER_PAIR_4_COMPL_END=0.00
5503
PRIMER_PRODUCT_SIZE_4=212
5504
=
5505
MARKER_NAME=24.RHAP61FH3.seq
5506
SEQUENCE=TAGAAATGNGACTGGTGAGATTTNANATTGTCCAANCATATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCATTTCAAACATTTCTGTGTGTGTTTCTCCAGATCTTCCTCTTTCTCTTCCTATACACATGAGAAAAGTANAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGCGTGCGGCGCGACACACACANATGGACAAATTGGNAAGANAGAAAGGATTNANTNNTTTAGACAACAGGTGTNNGAGATCNGGATTTTTCTCNTANANNTGTNGNCTGTTGNNAGGTNNCCAGGTGANTAGGGATNGGTGNNGANTTANNGGGGGGAGGTGCGNGGTNTNNCAGNNNNTTNATGGNTGGNGNTNGGTGAANNANANNTTCTCAGGNNNANNGNNGGGGGACGNATGTGGTTGGNNNNNNANNNNNANNNNNNTNTTGCNGNNGGNNNNNNGGNGNNTGNNNNTTTGNNTGANGGNNGGGGNGNNCNNCAAT
5507
INCLUDED_REGION=0,529
5508
TARGET=43,42,CA
5509
TARGET=162,44,CA
5510
PRIMER_COMMENT=CL is
5511
PRIMER_COMMENT=CR is
5512
=
5513
MARKER_NAME=26.RHAP61FA11.seq
5514
SEQUENCE=TNCCANCNTCCNATGCACCCACNCNGCCCCAGGGAANNACCGNNNACNCCCGNCTTCCNGNNCCCCATCNTCTGGCACGTNNACCCGNGNANATGCNNNNNNNNNNNNNNNNNCCCNNACNNACACACTNNAAATCNNTANAANANGNNTTNNATTCAGATAATACTATNNNCCNTGNTTNACACTTNCNNNNNCGNCATANTGCANATAACATGTTATGTAGGCATNTTGTCTGATGNAGNGGNACATGGCCCAGGCCCNAGTTNGGATCCTTAGNCACGNNTNANAGGNNNTGGGNATGNTNATTCNGCCCACAGGTNTTGGGNTCGTCANNANGGGGTACAGANNGNGANTTNGCNANNATGNNNATAAGGATGGNNNCNTGTGCGNNNTTGNTANNGNNANNNNTTTNCCAGGNNNANTANGGNGGGGGNNNNNTNNAGTGTNANT
5515
INCLUDED_REGION=0,450
5516
TARGET=99,14,CA
5517
PRIMER_COMMENT=CL is
5518
PRIMER_COMMENT=CR is
5519
=
5520
MARKER_NAME=26.RHAP61FA5.seq
5521
SEQUENCE=CTGTGTGAATTTCTAAGTCTTACCTTCAGAAGCAGTACCAGGTNGACATGAAAATACTGTCACTCTCTCAAATCATGTAATATTTCTGTTTGACTTGACATATGCAGAGAGTCTCTGTTTCAGCATAAGACATGCTAGCAGGCTTAAAAATACTGTTTCTGGCTGCTAAATCCTGAATCTACAAATGTGGGATCAATGATGTGAAAAGAGAAACTAAATGATGTGTGGAAGTTTATCAACTCACAGTGACAAGAAGCAGACTGCAGCAAGGTACCACACATTCAGGAAGTGAATGACTCCTTAAGTNATTCTCCTATAGAAATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCNTGNGATNTATNNGCCATGAATGTTTNTGNNTCTAGATACAAGAAAAGGGNTGGGTATTTNTGNGGCTCCNACAAGGGGCTTANTCACCAANTTTCTTTCNNGNNGAGGGGTTNCGGCNCGNATTT
5522
INCLUDED_REGION=0,508
5523
TARGET=324,56,CA
5524
PRIMER_COMMENT=CL is
5525
PRIMER_COMMENT=CR is
5526
=
5527
MARKER_NAME=27.RHAP61FA12.seq
5528
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCTCTGATGGAGCCTGTAGTCTAGCAGTATGTTTTCCTGAGACAAAAAAAGACACAGAAAAANATTGGAAGATTTAGCAATATGGAAGGATGCTGAAACCACAGGTATTCTTGGAATTCTTTCACTTACCTTAGTATGCCCCTTGTGNGGATTGAATGNNNTCCTGNAATACAGGCTGTCCTAGATTACAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAAAGGTTTTGGAGATGAAAAGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
5529
INCLUDED_REGION=21,258
5530
TARGET=213,42,GT
5531
PRIMER_COMMENT=CL is AAGGATGCTGAAACCACAGG
5532
PRIMER_COMMENT=CR is TCCTTTTCATCTCCAAAACCTT
5533
PRIMER_PAIR_PENALTY=3.0006
5534
PRIMER_LEFT_PENALTY=0.111077
5535
PRIMER_RIGHT_PENALTY=2.889543
5536
PRIMER_LEFT_SEQUENCE=AAGGATGCTGAAACCACAGG
5537
PRIMER_RIGHT_SEQUENCE=TCCTTTTCATCTCCAAAACCTT
5538
PRIMER_LEFT=105,20
5539
PRIMER_RIGHT=278,22
5540
PRIMER_LEFT_TM=60.111
5541
PRIMER_RIGHT_TM=59.110
5542
PRIMER_LEFT_GC_PERCENT=50.000
5543
PRIMER_RIGHT_GC_PERCENT=36.364
5544
PRIMER_LEFT_SELF_ANY=4.00
5545
PRIMER_RIGHT_SELF_ANY=4.00
5546
PRIMER_LEFT_SELF_END=1.00
5547
PRIMER_RIGHT_SELF_END=2.00
5548
PRIMER_LEFT_END_STABILITY=7.9000
5549
PRIMER_RIGHT_END_STABILITY=7.9000
5550
PRIMER_PAIR_COMPL_ANY=6.00
5551
PRIMER_PAIR_COMPL_END=2.00
5552
PRIMER_PRODUCT_SIZE=174
5553
PRIMER_PAIR_PENALTY_1=3.3103
5554
PRIMER_LEFT_1_PENALTY=0.420772
5555
PRIMER_RIGHT_1_PENALTY=2.889543
5556
PRIMER_LEFT_1_SEQUENCE=AGGATGCTGAAACCACAGGT
5557
PRIMER_RIGHT_1_SEQUENCE=TCCTTTTCATCTCCAAAACCTT
5558
PRIMER_LEFT_1=106,20
5559
PRIMER_RIGHT_1=278,22
5560
PRIMER_LEFT_1_TM=59.579
5561
PRIMER_RIGHT_1_TM=59.110
5562
PRIMER_LEFT_1_GC_PERCENT=50.000
5563
PRIMER_RIGHT_1_GC_PERCENT=36.364
5564
PRIMER_LEFT_1_SELF_ANY=4.00
5565
PRIMER_RIGHT_1_SELF_ANY=4.00
5566
PRIMER_LEFT_1_SELF_END=3.00
5567
PRIMER_RIGHT_1_SELF_END=2.00
5568
PRIMER_LEFT_1_END_STABILITY=7.9000
5569
PRIMER_RIGHT_1_END_STABILITY=7.9000
5570
PRIMER_PAIR_1_COMPL_ANY=6.00
5571
PRIMER_PAIR_1_COMPL_END=3.00
5572
PRIMER_PRODUCT_SIZE_1=173
5573
PRIMER_PAIR_PENALTY_2=3.3381
5574
PRIMER_LEFT_2_PENALTY=0.111077
5575
PRIMER_RIGHT_2_PENALTY=3.227003
5576
PRIMER_LEFT_2_SEQUENCE=AAGGATGCTGAAACCACAGG
5577
PRIMER_RIGHT_2_SEQUENCE=TCCTTTTCATCTCCAAAACCT
5578
PRIMER_LEFT_2=105,20
5579
PRIMER_RIGHT_2=278,21
5580
PRIMER_LEFT_2_TM=60.111
5581
PRIMER_RIGHT_2_TM=57.773
5582
PRIMER_LEFT_2_GC_PERCENT=50.000
5583
PRIMER_RIGHT_2_GC_PERCENT=38.095
5584
PRIMER_LEFT_2_SELF_ANY=4.00
5585
PRIMER_RIGHT_2_SELF_ANY=4.00
5586
PRIMER_LEFT_2_SELF_END=1.00
5587
PRIMER_RIGHT_2_SELF_END=0.00
5588
PRIMER_LEFT_2_END_STABILITY=7.9000
5589
PRIMER_RIGHT_2_END_STABILITY=7.9000
5590
PRIMER_PAIR_2_COMPL_ANY=6.00
5591
PRIMER_PAIR_2_COMPL_END=3.00
5592
PRIMER_PRODUCT_SIZE_2=174
5593
PRIMER_PAIR_PENALTY_3=3.4311
5594
PRIMER_LEFT_3_PENALTY=0.111077
5595
PRIMER_RIGHT_3_PENALTY=3.320073
5596
PRIMER_LEFT_3_SEQUENCE=AAGGATGCTGAAACCACAGG
5597
PRIMER_RIGHT_3_SEQUENCE=CCTTTTCATCTCCAAAACCTTTC
5598
PRIMER_LEFT_3=105,20
5599
PRIMER_RIGHT_3=277,23
5600
PRIMER_LEFT_3_TM=60.111
5601
PRIMER_RIGHT_3_TM=60.320
5602
PRIMER_LEFT_3_GC_PERCENT=50.000
5603
PRIMER_RIGHT_3_GC_PERCENT=39.130
5604
PRIMER_LEFT_3_SELF_ANY=4.00
5605
PRIMER_RIGHT_3_SELF_ANY=4.00
5606
PRIMER_LEFT_3_SELF_END=1.00
5607
PRIMER_RIGHT_3_SELF_END=2.00
5608
PRIMER_LEFT_3_END_STABILITY=7.9000
5609
PRIMER_RIGHT_3_END_STABILITY=7.0000
5610
PRIMER_PAIR_3_COMPL_ANY=6.00
5611
PRIMER_PAIR_3_COMPL_END=2.00
5612
PRIMER_PRODUCT_SIZE_3=173
5613
PRIMER_PAIR_PENALTY_4=3.4311
5614
PRIMER_LEFT_4_PENALTY=0.111077
5615
PRIMER_RIGHT_4_PENALTY=3.320073
5616
PRIMER_LEFT_4_SEQUENCE=AAGGATGCTGAAACCACAGG
5617
PRIMER_RIGHT_4_SEQUENCE=TCCTTTTCATCTCCAAAACCTTT
5618
PRIMER_LEFT_4=105,20
5619
PRIMER_RIGHT_4=278,23
5620
PRIMER_LEFT_4_TM=60.111
5621
PRIMER_RIGHT_4_TM=60.320
5622
PRIMER_LEFT_4_GC_PERCENT=50.000
5623
PRIMER_RIGHT_4_GC_PERCENT=34.783
5624
PRIMER_LEFT_4_SELF_ANY=4.00
5625
PRIMER_RIGHT_4_SELF_ANY=4.00
5626
PRIMER_LEFT_4_SELF_END=1.00
5627
PRIMER_RIGHT_4_SELF_END=4.00
5628
PRIMER_LEFT_4_END_STABILITY=7.9000
5629
PRIMER_RIGHT_4_END_STABILITY=8.5000
5630
PRIMER_PAIR_4_COMPL_ANY=6.00
5631
PRIMER_PAIR_4_COMPL_END=3.00
5632
PRIMER_PRODUCT_SIZE_4=174
5633
=
5634
MARKER_NAME=27.RHAP61FA6.seq
5635
SEQUENCE=GTAAAATGCGTANNAACCAGCACAANCAACACACTGTTTGAAGGCAGGAGAATTTTCTCTCATTCTGAGGAACATAAACTNTTTATTATGNACAGGCCTTTGGTAGAACCAGTATAATGTTTAATCAAATATTTGGGCATCTCATGCTCTGTGAGTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATCCCACAGACATCTAGAAGAATCTAGACAAGATAACAGAACATTNTCAACTAACTGAACCACCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
5636
INCLUDED_REGION=0,281
5637
TARGET=158,56,AC
5638
PRIMER_COMMENT=CL is CAGGCCTTTGGTAGAACCAG
5639
PRIMER_COMMENT=CR is TTCTTCTAGATGTCTGTGGGATG
5640
PRIMER_PAIR_PENALTY=4.9404
5641
PRIMER_LEFT_PENALTY=0.271680
5642
PRIMER_RIGHT_PENALTY=4.668729
5643
PRIMER_LEFT_SEQUENCE=CAGGCCTTTGGTAGAACCAG
5644
PRIMER_RIGHT_SEQUENCE=TTCTTCTAGATGTCTGTGGGATG
5645
PRIMER_LEFT=92,20
5646
PRIMER_RIGHT=236,23
5647
PRIMER_LEFT_TM=59.728
5648
PRIMER_RIGHT_TM=58.331
5649
PRIMER_LEFT_GC_PERCENT=55.000
5650
PRIMER_RIGHT_GC_PERCENT=43.478
5651
PRIMER_LEFT_SELF_ANY=6.00
5652
PRIMER_RIGHT_SELF_ANY=6.00
5653
PRIMER_LEFT_SELF_END=3.00
5654
PRIMER_RIGHT_SELF_END=0.00
5655
PRIMER_LEFT_END_STABILITY=7.9000
5656
PRIMER_RIGHT_END_STABILITY=8.1000
5657
PRIMER_PAIR_COMPL_ANY=6.00
5658
PRIMER_PAIR_COMPL_END=1.00
5659
PRIMER_PRODUCT_SIZE=145
5660
PRIMER_PAIR_PENALTY_1=5.0767
5661
PRIMER_LEFT_1_PENALTY=0.407941
5662
PRIMER_RIGHT_1_PENALTY=4.668729
5663
PRIMER_LEFT_1_SEQUENCE=ACAGGCCTTTGGTAGAACCA
5664
PRIMER_RIGHT_1_SEQUENCE=TTCTTCTAGATGTCTGTGGGATG
5665
PRIMER_LEFT_1=91,20
5666
PRIMER_RIGHT_1=236,23
5667
PRIMER_LEFT_1_TM=59.592
5668
PRIMER_RIGHT_1_TM=58.331
5669
PRIMER_LEFT_1_GC_PERCENT=50.000
5670
PRIMER_RIGHT_1_GC_PERCENT=43.478
5671
PRIMER_LEFT_1_SELF_ANY=6.00
5672
PRIMER_RIGHT_1_SELF_ANY=6.00
5673
PRIMER_LEFT_1_SELF_END=5.00
5674
PRIMER_RIGHT_1_SELF_END=0.00
5675
PRIMER_LEFT_1_END_STABILITY=8.2000
5676
PRIMER_RIGHT_1_END_STABILITY=8.1000
5677
PRIMER_PAIR_1_COMPL_ANY=7.00
5678
PRIMER_PAIR_1_COMPL_END=2.00
5679
PRIMER_PRODUCT_SIZE_1=146
5680
PRIMER_PAIR_PENALTY_2=5.5996
5681
PRIMER_LEFT_2_PENALTY=0.271680
5682
PRIMER_RIGHT_2_PENALTY=5.327920
5683
PRIMER_LEFT_2_SEQUENCE=CAGGCCTTTGGTAGAACCAG
5684
PRIMER_RIGHT_2_SEQUENCE=ATTCTTCTAGATGTCTGTGGGATG
5685
PRIMER_LEFT_2=92,20
5686
PRIMER_RIGHT_2=237,24
5687
PRIMER_LEFT_2_TM=59.728
5688
PRIMER_RIGHT_2_TM=58.672
5689
PRIMER_LEFT_2_GC_PERCENT=55.000
5690
PRIMER_RIGHT_2_GC_PERCENT=41.667
5691
PRIMER_LEFT_2_SELF_ANY=6.00
5692
PRIMER_RIGHT_2_SELF_ANY=6.00
5693
PRIMER_LEFT_2_SELF_END=3.00
5694
PRIMER_RIGHT_2_SELF_END=0.00
5695
PRIMER_LEFT_2_END_STABILITY=7.9000
5696
PRIMER_RIGHT_2_END_STABILITY=8.1000
5697
PRIMER_PAIR_2_COMPL_ANY=6.00
5698
PRIMER_PAIR_2_COMPL_END=1.00
5699
PRIMER_PRODUCT_SIZE_2=146
5700
PRIMER_PAIR_PENALTY_3=5.6451
5701
PRIMER_LEFT_3_PENALTY=0.271680
5702
PRIMER_RIGHT_3_PENALTY=5.373459
5703
PRIMER_LEFT_3_SEQUENCE=CAGGCCTTTGGTAGAACCAG
5704
PRIMER_RIGHT_3_SEQUENCE=GATTCTTCTAGATGTCTGTGGGATG
5705
PRIMER_LEFT_3=92,20
5706
PRIMER_RIGHT_3=238,25
5707
PRIMER_LEFT_3_TM=59.728
5708
PRIMER_RIGHT_3_TM=60.373
5709
PRIMER_LEFT_3_GC_PERCENT=55.000
5710
PRIMER_RIGHT_3_GC_PERCENT=44.000
5711
PRIMER_LEFT_3_SELF_ANY=6.00
5712
PRIMER_RIGHT_3_SELF_ANY=6.00
5713
PRIMER_LEFT_3_SELF_END=3.00
5714
PRIMER_RIGHT_3_SELF_END=0.00
5715
PRIMER_LEFT_3_END_STABILITY=7.9000
5716
PRIMER_RIGHT_3_END_STABILITY=8.1000
5717
PRIMER_PAIR_3_COMPL_ANY=6.00
5718
PRIMER_PAIR_3_COMPL_END=1.00
5719
PRIMER_PRODUCT_SIZE_3=147
5720
PRIMER_PAIR_PENALTY_4=5.7359
5721
PRIMER_LEFT_4_PENALTY=0.407941
5722
PRIMER_RIGHT_4_PENALTY=5.327920
5723
PRIMER_LEFT_4_SEQUENCE=ACAGGCCTTTGGTAGAACCA
5724
PRIMER_RIGHT_4_SEQUENCE=ATTCTTCTAGATGTCTGTGGGATG
5725
PRIMER_LEFT_4=91,20
5726
PRIMER_RIGHT_4=237,24
5727
PRIMER_LEFT_4_TM=59.592
5728
PRIMER_RIGHT_4_TM=58.672
5729
PRIMER_LEFT_4_GC_PERCENT=50.000
5730
PRIMER_RIGHT_4_GC_PERCENT=41.667
5731
PRIMER_LEFT_4_SELF_ANY=6.00
5732
PRIMER_RIGHT_4_SELF_ANY=6.00
5733
PRIMER_LEFT_4_SELF_END=5.00
5734
PRIMER_RIGHT_4_SELF_END=0.00
5735
PRIMER_LEFT_4_END_STABILITY=8.2000
5736
PRIMER_RIGHT_4_END_STABILITY=8.1000
5737
PRIMER_PAIR_4_COMPL_ANY=7.00
5738
PRIMER_PAIR_4_COMPL_END=2.00
5739
PRIMER_PRODUCT_SIZE_4=147
5740
=
5741
MARKER_NAME=29.RHAP61FB11.seq
5742
SEQUENCE=GGCNNTATAGANNATCCCNTCNGGNCATNTCAANCCCACATNCTCTTANAANAACTTTGNAAAATGATGNNTGANNAAAANTTACTGCTNNNGNNNNNTCNACTTTGGNGNGNCTCTGNCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATANGTGTCNATTTANNATGCANANCTNNNTCGGAGGTTGTGNTGNNCNCTCNAAAACTCNTTNTNNNTNCNTCCAGGGGNNGGGNNCNNCCGNNTNNNTCTCGNNCNTTTNNANATANGNANGTAANTCTGGNATCNTCCNANTNNACNNNTNAGNGAANNATGGGTNNNNGNAAGNGCTTTTNTNNNANGAGGGGGGGNGNNNNNGANNTGGAATGGGNANNNNGGGNNNAAAANNNGTTTTCCNTTGGNAANTTNNNNTCNNNNNNGANTNNNANAANAANATTNGAGNGGGAANCNNNAANNNTAAAGCNNGGGGNNCTT
5743
INCLUDED_REGION=0,490
5744
TARGET=123,40,GT
5745
PRIMER_COMMENT=CL is
5746
PRIMER_COMMENT=CR is
5747
=
5748
MARKER_NAME=30.RHAP61FB12.seq
5749
SEQUENCE=NNNNNNNNNNNNNNNNNNNTGCCTGAAAAAAGAAAATCACACTTTTTGATCTCTAGCCACCTAAAATGTTTGCTAAGTTTCACTTCCCAACACCAGGGGGTGTCTCTTCGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTTGTCCTTAAGGTTCCATTAGTCCCAAACCTTTCTCCGTGTTTTTATGTACACTCTAAATCTTTCTCTGTCCCTCCAGAGGCAGGGATCAGCCTTATTTTCCTCGCTCTTTTTCATATAAGCACATAAATCTTGTATCCCCCCACTCCACCNNCAGAGAACCATGGGTCTAGAAAGTGCTTTTTATTGAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
5750
INCLUDED_REGION=19,332
5751
TARGET=112,46,TG
5752
PRIMER_COMMENT=CL is AACACCAGGGGGTGTCTCTT
5753
PRIMER_COMMENT=CR is AATAAGGCTGATCCCTGCCT
5754
PRIMER_PAIR_PENALTY=0.8615
5755
PRIMER_LEFT_PENALTY=0.801345
5756
PRIMER_RIGHT_PENALTY=0.060196
5757
PRIMER_LEFT_SEQUENCE=AACACCAGGGGGTGTCTCTT
5758
PRIMER_RIGHT_SEQUENCE=AATAAGGCTGATCCCTGCCT
5759
PRIMER_LEFT=88,20
5760
PRIMER_RIGHT=257,20
5761
PRIMER_LEFT_TM=60.801
5762
PRIMER_RIGHT_TM=60.060
5763
PRIMER_LEFT_GC_PERCENT=55.000
5764
PRIMER_RIGHT_GC_PERCENT=50.000
5765
PRIMER_LEFT_SELF_ANY=6.00
5766
PRIMER_RIGHT_SELF_ANY=5.00
5767
PRIMER_LEFT_SELF_END=0.00
5768
PRIMER_RIGHT_SELF_END=4.00
5769
PRIMER_LEFT_END_STABILITY=6.7000
5770
PRIMER_RIGHT_END_STABILITY=9.7000
5771
PRIMER_PAIR_COMPL_ANY=5.00
5772
PRIMER_PAIR_COMPL_END=0.00
5773
PRIMER_PRODUCT_SIZE=170
5774
PRIMER_PAIR_PENALTY_1=1.1562
5775
PRIMER_LEFT_1_PENALTY=1.096054
5776
PRIMER_RIGHT_1_PENALTY=0.060196
5777
PRIMER_LEFT_1_SEQUENCE=CAGGGGGTGTCTCTTCGTT
5778
PRIMER_RIGHT_1_SEQUENCE=AATAAGGCTGATCCCTGCCT
5779
PRIMER_LEFT_1=93,19
5780
PRIMER_RIGHT_1=257,20
5781
PRIMER_LEFT_1_TM=60.096
5782
PRIMER_RIGHT_1_TM=60.060
5783
PRIMER_LEFT_1_GC_PERCENT=57.895
5784
PRIMER_RIGHT_1_GC_PERCENT=50.000
5785
PRIMER_LEFT_1_SELF_ANY=2.00
5786
PRIMER_RIGHT_1_SELF_ANY=5.00
5787
PRIMER_LEFT_1_SELF_END=0.00
5788
PRIMER_RIGHT_1_SELF_END=4.00
5789
PRIMER_LEFT_1_END_STABILITY=8.4000
5790
PRIMER_RIGHT_1_END_STABILITY=9.7000
5791
PRIMER_PAIR_1_COMPL_ANY=5.00
5792
PRIMER_PAIR_1_COMPL_END=1.00
5793
PRIMER_PRODUCT_SIZE_1=165
5794
PRIMER_PAIR_PENALTY_2=1.2205
5795
PRIMER_LEFT_2_PENALTY=0.801345
5796
PRIMER_RIGHT_2_PENALTY=0.419105
5797
PRIMER_LEFT_2_SEQUENCE=AACACCAGGGGGTGTCTCTT
5798
PRIMER_RIGHT_2_SEQUENCE=AAATAAGGCTGATCCCTGCC
5799
PRIMER_LEFT_2=88,20
5800
PRIMER_RIGHT_2=258,20
5801
PRIMER_LEFT_2_TM=60.801
5802
PRIMER_RIGHT_2_TM=60.419
5803
PRIMER_LEFT_2_GC_PERCENT=55.000
5804
PRIMER_RIGHT_2_GC_PERCENT=50.000
5805
PRIMER_LEFT_2_SELF_ANY=6.00
5806
PRIMER_RIGHT_2_SELF_ANY=5.00
5807
PRIMER_LEFT_2_SELF_END=0.00
5808
PRIMER_RIGHT_2_SELF_END=2.00
5809
PRIMER_LEFT_2_END_STABILITY=6.7000
5810
PRIMER_RIGHT_2_END_STABILITY=9.7000
5811
PRIMER_PAIR_2_COMPL_ANY=5.00
5812
PRIMER_PAIR_2_COMPL_END=0.00
5813
PRIMER_PRODUCT_SIZE_2=171
5814
PRIMER_PAIR_PENALTY_3=1.3683
5815
PRIMER_LEFT_3_PENALTY=0.801345
5816
PRIMER_RIGHT_3_PENALTY=0.566932
5817
PRIMER_LEFT_3_SEQUENCE=AACACCAGGGGGTGTCTCTT
5818
PRIMER_RIGHT_3_SEQUENCE=ATAAGGCTGATCCCTGCCTC
5819
PRIMER_LEFT_3=88,20
5820
PRIMER_RIGHT_3=256,20
5821
PRIMER_LEFT_3_TM=60.801
5822
PRIMER_RIGHT_3_TM=60.567
5823
PRIMER_LEFT_3_GC_PERCENT=55.000
5824
PRIMER_RIGHT_3_GC_PERCENT=55.000
5825
PRIMER_LEFT_3_SELF_ANY=6.00
5826
PRIMER_RIGHT_3_SELF_ANY=5.00
5827
PRIMER_LEFT_3_SELF_END=0.00
5828
PRIMER_RIGHT_3_SELF_END=2.00
5829
PRIMER_LEFT_3_END_STABILITY=6.7000
5830
PRIMER_RIGHT_3_END_STABILITY=9.4000
5831
PRIMER_PAIR_3_COMPL_ANY=5.00
5832
PRIMER_PAIR_3_COMPL_END=0.00
5833
PRIMER_PRODUCT_SIZE_3=169
5834
PRIMER_PAIR_PENALTY_4=1.4239
5835
PRIMER_LEFT_4_PENALTY=1.363742
5836
PRIMER_RIGHT_4_PENALTY=0.060196
5837
PRIMER_LEFT_4_SEQUENCE=ACACCAGGGGGTGTCTCTTC
5838
PRIMER_RIGHT_4_SEQUENCE=AATAAGGCTGATCCCTGCCT
5839
PRIMER_LEFT_4=89,20
5840
PRIMER_RIGHT_4=257,20
5841
PRIMER_LEFT_4_TM=61.364
5842
PRIMER_RIGHT_4_TM=60.060
5843
PRIMER_LEFT_4_GC_PERCENT=60.000
5844
PRIMER_RIGHT_4_GC_PERCENT=50.000
5845
PRIMER_LEFT_4_SELF_ANY=6.00
5846
PRIMER_RIGHT_4_SELF_ANY=5.00
5847
PRIMER_LEFT_4_SELF_END=0.00
5848
PRIMER_RIGHT_4_SELF_END=4.00
5849
PRIMER_LEFT_4_END_STABILITY=6.7000
5850
PRIMER_RIGHT_4_END_STABILITY=9.7000
5851
PRIMER_PAIR_4_COMPL_ANY=5.00
5852
PRIMER_PAIR_4_COMPL_END=0.00
5853
PRIMER_PRODUCT_SIZE_4=169
5854
=
5855
MARKER_NAME=31.RHAP61FC4.seq
5856
SEQUENCE=CCTNCCCCNCNNCNNTGGNCCCNCTCAANNNNNNNNNNNNNNNNNNTCTNCANNNAGNNCGNNACCNTCNATCNCNAGNCANGNTCCNTCCCNNNAACNANNNTCNTCTATAGAAAACNCNCTGGNCGCNCTACGATNNGNNCNNACNNTNNNANNNTNGNNNTNNAANGGNCNNNNNNNGCNNCGNNGNNCNNACNNNCNNNTNNANNTNNCTNNNTNNNNTCGNNNNGNNCNNTNGNNNNGNNNCNNGCTNCNTGGCTTNNNANNNNNCCGACNNNNNNACCNTTTNCNNTNTTTNNGCNTGCNNCNCGNNCCTCNGGNNNNNNNGNNNNNNNNNTNNNGNTTNCNNNCGNGNNGGCNNNNTNNNNNGNNNNGNNNGNNNNGNTCGGNNNCCGNTNNGNNNNCCCNNCCACNNNCCGNTCGNNNNNCNCNNNCNNGCNNCNACCNCNCGGNNNNCNNNGNNNCNNNCNNCNCNGNNTGGNGCGNNCGGCNNNCNGNNGNTGNNNNNNNCNNCCNGNNNNNNNGNTCNNNNGTNGGGNNTCCNNNCCNNTNCNNNNNNNNNCNNNCNGNNNCGNNNGGC
5857
INCLUDED_REGION=0,580
5858
TARGET=29,17,NC
5859
PRIMER_COMMENT=CL is
5860
PRIMER_COMMENT=CR is
5861
=
5862
MARKER_NAME=33.RHAP61FC12.seq
5863
SEQUENCE=TCCACATNNCAAGTTCCAGGTCAACCAGGGTTNNGTGGTGAGACCTCCCCCTTCTCTTACGCACACGCCAAACCTTAACAAACCCCCTAAAAGCCAAACCNTGAGAAAACAGAAACAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCANNTCANAGTCNCTTCNAGANTTGCATACCTAATATGNGCCNTTATCTATGTNNGCAAATGAANGTGAGTCTATANTTTNTNATACCANNGGGGGAGCNTTTGGGCNGCCCAGGGAGCTTTGGTGGGAGAGNATANAGNGGGGNGNNCNNNTCNTGGNAANNAGGAAATCTCNTTNNGNNNNTTNNTGNTANGTCCNGGAGTNTGTGTATNNNATCCTTNNGNTTTNTNNTNNGANATCNNTCCNCTAGGGNTCTTGGTCNNGGGTCNNNGTNNNNNNCGGGTGGAAAAT
5864
INCLUDED_REGION=0,469
5865
TARGET=118,60,AC
5866
PRIMER_COMMENT=CL is GACCTCCCCCTTCTCTTACG
5867
PRIMER_COMMENT=CR is CTCCCACCAAAGCTCCCT
5868
PRIMER_PAIR_PENALTY=2.2576
5869
PRIMER_LEFT_PENALTY=0.066676
5870
PRIMER_RIGHT_PENALTY=2.190960
5871
PRIMER_LEFT_SEQUENCE=GACCTCCCCCTTCTCTTACG
5872
PRIMER_RIGHT_SEQUENCE=CTCCCACCAAAGCTCCCT
5873
PRIMER_LEFT=41,20
5874
PRIMER_RIGHT=307,18
5875
PRIMER_LEFT_TM=60.067
5876
PRIMER_RIGHT_TM=60.191
5877
PRIMER_LEFT_GC_PERCENT=60.000
5878
PRIMER_RIGHT_GC_PERCENT=61.111
5879
PRIMER_LEFT_SELF_ANY=2.00
5880
PRIMER_RIGHT_SELF_ANY=4.00
5881
PRIMER_LEFT_SELF_END=2.00
5882
PRIMER_RIGHT_SELF_END=0.00
5883
PRIMER_LEFT_END_STABILITY=7.7000
5884
PRIMER_RIGHT_END_STABILITY=9.4000
5885
PRIMER_PAIR_COMPL_ANY=3.00
5886
PRIMER_PAIR_COMPL_END=1.00
5887
PRIMER_PRODUCT_SIZE=267
5888
PRIMER_PAIR_PENALTY_1=2.3769
5889
PRIMER_LEFT_1_PENALTY=0.185942
5890
PRIMER_RIGHT_1_PENALTY=2.190960
5891
PRIMER_LEFT_1_SEQUENCE=TGAGACCTCCCCCTTCTCTT
5892
PRIMER_RIGHT_1_SEQUENCE=CTCCCACCAAAGCTCCCT
5893
PRIMER_LEFT_1=38,20
5894
PRIMER_RIGHT_1=307,18
5895
PRIMER_LEFT_1_TM=60.186
5896
PRIMER_RIGHT_1_TM=60.191
5897
PRIMER_LEFT_1_GC_PERCENT=55.000
5898
PRIMER_RIGHT_1_GC_PERCENT=61.111
5899
PRIMER_LEFT_1_SELF_ANY=4.00
5900
PRIMER_RIGHT_1_SELF_ANY=4.00
5901
PRIMER_LEFT_1_SELF_END=0.00
5902
PRIMER_RIGHT_1_SELF_END=0.00
5903
PRIMER_LEFT_1_END_STABILITY=6.7000
5904
PRIMER_RIGHT_1_END_STABILITY=9.4000
5905
PRIMER_PAIR_1_COMPL_ANY=3.00
5906
PRIMER_PAIR_1_COMPL_END=0.00
5907
PRIMER_PRODUCT_SIZE_1=270
5908
PRIMER_PAIR_PENALTY_2=2.5298
5909
PRIMER_LEFT_2_PENALTY=0.338810
5910
PRIMER_RIGHT_2_PENALTY=2.190960
5911
PRIMER_LEFT_2_SEQUENCE=GTGAGACCTCCCCCTTCTCT
5912
PRIMER_RIGHT_2_SEQUENCE=CTCCCACCAAAGCTCCCT
5913
PRIMER_LEFT_2=37,20
5914
PRIMER_RIGHT_2=307,18
5915
PRIMER_LEFT_2_TM=59.661
5916
PRIMER_RIGHT_2_TM=60.191
5917
PRIMER_LEFT_2_GC_PERCENT=60.000
5918
PRIMER_RIGHT_2_GC_PERCENT=61.111
5919
PRIMER_LEFT_2_SELF_ANY=4.00
5920
PRIMER_RIGHT_2_SELF_ANY=4.00
5921
PRIMER_LEFT_2_SELF_END=0.00
5922
PRIMER_RIGHT_2_SELF_END=0.00
5923
PRIMER_LEFT_2_END_STABILITY=6.4000
5924
PRIMER_RIGHT_2_END_STABILITY=9.4000
5925
PRIMER_PAIR_2_COMPL_ANY=4.00
5926
PRIMER_PAIR_2_COMPL_END=0.00
5927
PRIMER_PRODUCT_SIZE_2=271
5928
PRIMER_PAIR_PENALTY_3=2.5643
5929
PRIMER_LEFT_3_PENALTY=0.373358
5930
PRIMER_RIGHT_3_PENALTY=2.190960
5931
PRIMER_LEFT_3_SEQUENCE=CACACGCCAAACCTTAACAA
5932
PRIMER_RIGHT_3_SEQUENCE=CTCCCACCAAAGCTCCCT
5933
PRIMER_LEFT_3=61,20
5934
PRIMER_RIGHT_3=307,18
5935
PRIMER_LEFT_3_TM=59.627
5936
PRIMER_RIGHT_3_TM=60.191
5937
PRIMER_LEFT_3_GC_PERCENT=45.000
5938
PRIMER_RIGHT_3_GC_PERCENT=61.111
5939
PRIMER_LEFT_3_SELF_ANY=4.00
5940
PRIMER_RIGHT_3_SELF_ANY=4.00
5941
PRIMER_LEFT_3_SELF_END=1.00
5942
PRIMER_RIGHT_3_SELF_END=0.00
5943
PRIMER_LEFT_3_END_STABILITY=7.0000
5944
PRIMER_RIGHT_3_END_STABILITY=9.4000
5945
PRIMER_PAIR_3_COMPL_ANY=3.00
5946
PRIMER_PAIR_3_COMPL_END=1.00
5947
PRIMER_PRODUCT_SIZE_3=247
5948
PRIMER_PAIR_PENALTY_4=2.6257
5949
PRIMER_LEFT_4_PENALTY=0.434743
5950
PRIMER_RIGHT_4_PENALTY=2.190960
5951
PRIMER_LEFT_4_SEQUENCE=CCAAACCTTAACAAACCCCC
5952
PRIMER_RIGHT_4_SEQUENCE=CTCCCACCAAAGCTCCCT
5953
PRIMER_LEFT_4=67,20
5954
PRIMER_RIGHT_4=307,18
5955
PRIMER_LEFT_4_TM=60.435
5956
PRIMER_RIGHT_4_TM=60.191
5957
PRIMER_LEFT_4_GC_PERCENT=50.000
5958
PRIMER_RIGHT_4_GC_PERCENT=61.111
5959
PRIMER_LEFT_4_SELF_ANY=4.00
5960
PRIMER_RIGHT_4_SELF_ANY=4.00
5961
PRIMER_LEFT_4_SELF_END=0.00
5962
PRIMER_RIGHT_4_SELF_END=0.00
5963
PRIMER_LEFT_4_END_STABILITY=12.4000
5964
PRIMER_RIGHT_4_END_STABILITY=9.4000
5965
PRIMER_PAIR_4_COMPL_ANY=3.00
5966
PRIMER_PAIR_4_COMPL_END=0.00
5967
PRIMER_PRODUCT_SIZE_4=241
5968
=
5969
MARKER_NAME=34.RHAP61FD10.seq
5970
SEQUENCE=NNNNNNNNNNNNNNNNNNNCTGGAGGATTTCTTTTTCTCTGATAGTATTTAATGTTTCTTTTTCTCTTCTTCAATTGAGGTTCCCTTTTCCTGTGTAAGTCAGAGTTCTTATTTCCACTTCAGTCTCTCAGACAGTTTCGATTACANNNNNNNNNNNNNNNNCCACTTAGATTTCTATTCAGGACAGAAGTCAAATTACTTTACTCGGCTGTTAATGCTTTTCAATTGCACAGAAAAAGGCTCTAGATCTTTGGTACCGAGTTTGCTAACCAATGTGACAGACCCTTTGTTTTAAAATTATGAACGGGGGAAAACAAATAGAGGAAATAAATTCNNAATAGCATGAATTTATACTTCCNGAAATGACCAAAGGGAAAGCCAGGTCCCATNATTACCTTGNGATGCAGAGCAGCNGGNTCAGNACAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
5971
INCLUDED_REGION=19,411
5972
TARGET=146,16,TG
5973
PRIMER_COMMENT=CL is TGAGGTTCCCTTTTCCTGTG
5974
PRIMER_COMMENT=CR is TGAAAAGCATTAACAGCCGA
5975
PRIMER_PAIR_PENALTY=0.6372
5976
PRIMER_LEFT_PENALTY=0.080729
5977
PRIMER_RIGHT_PENALTY=0.556510
5978
PRIMER_LEFT_SEQUENCE=TGAGGTTCCCTTTTCCTGTG
5979
PRIMER_RIGHT_SEQUENCE=TGAAAAGCATTAACAGCCGA
5980
PRIMER_LEFT=75,20
5981
PRIMER_RIGHT=223,20
5982
PRIMER_LEFT_TM=60.081
5983
PRIMER_RIGHT_TM=59.443
5984
PRIMER_LEFT_GC_PERCENT=50.000
5985
PRIMER_RIGHT_GC_PERCENT=40.000
5986
PRIMER_LEFT_SELF_ANY=3.00
5987
PRIMER_RIGHT_SELF_ANY=4.00
5988
PRIMER_LEFT_SELF_END=0.00
5989
PRIMER_RIGHT_SELF_END=0.00
5990
PRIMER_LEFT_END_STABILITY=6.7000
5991
PRIMER_RIGHT_END_STABILITY=11.4000
5992
PRIMER_PAIR_COMPL_ANY=6.00
5993
PRIMER_PAIR_COMPL_END=1.00
5994
PRIMER_PRODUCT_SIZE=149
5995
PRIMER_PAIR_PENALTY_1=1.1232
5996
PRIMER_LEFT_1_PENALTY=0.080729
5997
PRIMER_RIGHT_1_PENALTY=1.042469
5998
PRIMER_LEFT_1_SEQUENCE=TGAGGTTCCCTTTTCCTGTG
5999
PRIMER_RIGHT_1_SEQUENCE=TTGAAAAGCATTAACAGCCG
6000
PRIMER_LEFT_1=75,20
6001
PRIMER_RIGHT_1=224,20
6002
PRIMER_LEFT_1_TM=60.081
6003
PRIMER_RIGHT_1_TM=58.958
6004
PRIMER_LEFT_1_GC_PERCENT=50.000
6005
PRIMER_RIGHT_1_GC_PERCENT=40.000
6006
PRIMER_LEFT_1_SELF_ANY=3.00
6007
PRIMER_RIGHT_1_SELF_ANY=4.00
6008
PRIMER_LEFT_1_SELF_END=0.00
6009
PRIMER_RIGHT_1_SELF_END=2.00
6010
PRIMER_LEFT_1_END_STABILITY=6.7000
6011
PRIMER_RIGHT_1_END_STABILITY=11.4000
6012
PRIMER_PAIR_1_COMPL_ANY=6.00
6013
PRIMER_PAIR_1_COMPL_END=1.00
6014
PRIMER_PRODUCT_SIZE_1=150
6015
PRIMER_PAIR_PENALTY_2=1.1861
6016
PRIMER_LEFT_2_PENALTY=0.080729
6017
PRIMER_RIGHT_2_PENALTY=1.105391
6018
PRIMER_LEFT_2_SEQUENCE=TGAGGTTCCCTTTTCCTGTG
6019
PRIMER_RIGHT_2_SEQUENCE=GCCTTTTTCTGTGCAATTGA
6020
PRIMER_LEFT_2=75,20
6021
PRIMER_RIGHT_2=240,20
6022
PRIMER_LEFT_2_TM=60.081
6023
PRIMER_RIGHT_2_TM=58.895
6024
PRIMER_LEFT_2_GC_PERCENT=50.000
6025
PRIMER_RIGHT_2_GC_PERCENT=40.000
6026
PRIMER_LEFT_2_SELF_ANY=3.00
6027
PRIMER_RIGHT_2_SELF_ANY=6.00
6028
PRIMER_LEFT_2_SELF_END=0.00
6029
PRIMER_RIGHT_2_SELF_END=4.00
6030
PRIMER_LEFT_2_END_STABILITY=6.7000
6031
PRIMER_RIGHT_2_END_STABILITY=6.9000
6032
PRIMER_PAIR_2_COMPL_ANY=3.00
6033
PRIMER_PAIR_2_COMPL_END=1.00
6034
PRIMER_PRODUCT_SIZE_2=166
6035
PRIMER_PAIR_PENALTY_3=1.2058
6036
PRIMER_LEFT_3_PENALTY=0.080729
6037
PRIMER_RIGHT_3_PENALTY=1.125045
6038
PRIMER_LEFT_3_SEQUENCE=TGAGGTTCCCTTTTCCTGTG
6039
PRIMER_RIGHT_3_SEQUENCE=AGCCTTTTTCTGTGCAATTGA
6040
PRIMER_LEFT_3=75,20
6041
PRIMER_RIGHT_3=241,21
6042
PRIMER_LEFT_3_TM=60.081
6043
PRIMER_RIGHT_3_TM=59.875
6044
PRIMER_LEFT_3_GC_PERCENT=50.000
6045
PRIMER_RIGHT_3_GC_PERCENT=38.095
6046
PRIMER_LEFT_3_SELF_ANY=3.00
6047
PRIMER_RIGHT_3_SELF_ANY=6.00
6048
PRIMER_LEFT_3_SELF_END=0.00
6049
PRIMER_RIGHT_3_SELF_END=4.00
6050
PRIMER_LEFT_3_END_STABILITY=6.7000
6051
PRIMER_RIGHT_3_END_STABILITY=6.9000
6052
PRIMER_PAIR_3_COMPL_ANY=3.00
6053
PRIMER_PAIR_3_COMPL_END=1.00
6054
PRIMER_PRODUCT_SIZE_3=167
6055
PRIMER_PAIR_PENALTY_4=1.2058
6056
PRIMER_LEFT_4_PENALTY=0.080729
6057
PRIMER_RIGHT_4_PENALTY=1.125045
6058
PRIMER_LEFT_4_SEQUENCE=TGAGGTTCCCTTTTCCTGTG
6059
PRIMER_RIGHT_4_SEQUENCE=GAGCCTTTTTCTGTGCAATTG
6060
PRIMER_LEFT_4=75,20
6061
PRIMER_RIGHT_4=242,21
6062
PRIMER_LEFT_4_TM=60.081
6063
PRIMER_RIGHT_4_TM=59.875
6064
PRIMER_LEFT_4_GC_PERCENT=50.000
6065
PRIMER_RIGHT_4_GC_PERCENT=42.857
6066
PRIMER_LEFT_4_SELF_ANY=3.00
6067
PRIMER_RIGHT_4_SELF_ANY=6.00
6068
PRIMER_LEFT_4_SELF_END=0.00
6069
PRIMER_RIGHT_4_SELF_END=6.00
6070
PRIMER_LEFT_4_END_STABILITY=6.7000
6071
PRIMER_RIGHT_4_END_STABILITY=7.2000
6072
PRIMER_PAIR_4_COMPL_ANY=4.00
6073
PRIMER_PAIR_4_COMPL_END=0.00
6074
PRIMER_PRODUCT_SIZE_4=168
6075
=
6076
MARKER_NAME=36.RHAP61FD12.seq
6077
SEQUENCE=NNNNNNNNNNNNNNNNNNNNCTCAGCCCGTGTCATTTGACGCTTTCTTAGCACAATATTCCAGACTCTTTCACCTTTTGGTTATGATATAGTTCAAGCAGTTAAAAATCACGGAGGTTCATCACAGAAAGGGTGATGCTTGCTTTTTTTGGTACCAAGTTCTANCNTTGTTTAATTTCTTTGACCTTCCTCTACAATCTCATTTATTTTCAGTCAGGCGAGTGTCTGTGAGTGNGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCGCACTCATGTGCATACACACAGACGTTAGAAAAAGCCAGTTCCCCTAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
6078
INCLUDED_REGION=20,315
6079
TARGET=236,48,GT
6080
PRIMER_COMMENT=CL is TTCAGTCAGGCGAGTGTCTG
6081
PRIMER_COMMENT=CR is TCTAGGGGAACTGGCTTTTTC
6082
PRIMER_PAIR_PENALTY=1.4666
6083
PRIMER_LEFT_PENALTY=0.178378
6084
PRIMER_RIGHT_PENALTY=1.288173
6085
PRIMER_LEFT_SEQUENCE=TTCAGTCAGGCGAGTGTCTG
6086
PRIMER_RIGHT_SEQUENCE=TCTAGGGGAACTGGCTTTTTC
6087
PRIMER_LEFT=207,20
6088
PRIMER_RIGHT=334,21
6089
PRIMER_LEFT_TM=60.178
6090
PRIMER_RIGHT_TM=59.712
6091
PRIMER_LEFT_GC_PERCENT=55.000
6092
PRIMER_RIGHT_GC_PERCENT=47.619
6093
PRIMER_LEFT_SELF_ANY=6.00
6094
PRIMER_RIGHT_SELF_ANY=4.00
6095
PRIMER_LEFT_SELF_END=6.00
6096
PRIMER_RIGHT_SELF_END=2.00
6097
PRIMER_LEFT_END_STABILITY=6.4000
6098
PRIMER_RIGHT_END_STABILITY=7.3000
6099
PRIMER_PAIR_COMPL_ANY=4.00
6100
PRIMER_PAIR_COMPL_END=1.00
6101
PRIMER_PRODUCT_SIZE=128
6102
PRIMER_PAIR_PENALTY_1=1.5147
6103
PRIMER_LEFT_1_PENALTY=0.794652
6104
PRIMER_RIGHT_1_PENALTY=0.720013
6105
PRIMER_LEFT_1_SEQUENCE=GGGTGATGCTTGCTTTTTTT
6106
PRIMER_RIGHT_1_SEQUENCE=GTGTATGCACATGAGTGCGA
6107
PRIMER_LEFT_1=129,20
6108
PRIMER_RIGHT_1=303,20
6109
PRIMER_LEFT_1_TM=59.205
6110
PRIMER_RIGHT_1_TM=59.280
6111
PRIMER_LEFT_1_GC_PERCENT=40.000
6112
PRIMER_RIGHT_1_GC_PERCENT=50.000
6113
PRIMER_LEFT_1_SELF_ANY=2.00
6114
PRIMER_RIGHT_1_SELF_ANY=6.00
6115
PRIMER_LEFT_1_SELF_END=0.00
6116
PRIMER_RIGHT_1_SELF_END=3.00
6117
PRIMER_LEFT_1_END_STABILITY=7.6000
6118
PRIMER_RIGHT_1_END_STABILITY=10.2000
6119
PRIMER_PAIR_1_COMPL_ANY=3.00
6120
PRIMER_PAIR_1_COMPL_END=1.00
6121
PRIMER_PRODUCT_SIZE_1=175
6122
PRIMER_PAIR_PENALTY_2=1.6848
6123
PRIMER_LEFT_2_PENALTY=0.396662
6124
PRIMER_RIGHT_2_PENALTY=1.288173
6125
PRIMER_LEFT_2_SEQUENCE=AGTCAGGCGAGTGTCTGTGA
6126
PRIMER_RIGHT_2_SEQUENCE=TCTAGGGGAACTGGCTTTTTC
6127
PRIMER_LEFT_2=210,20
6128
PRIMER_RIGHT_2=334,21
6129
PRIMER_LEFT_2_TM=59.603
6130
PRIMER_RIGHT_2_TM=59.712
6131
PRIMER_LEFT_2_GC_PERCENT=55.000
6132
PRIMER_RIGHT_2_GC_PERCENT=47.619
6133
PRIMER_LEFT_2_SELF_ANY=4.00
6134
PRIMER_RIGHT_2_SELF_ANY=4.00
6135
PRIMER_LEFT_2_SELF_END=1.00
6136
PRIMER_RIGHT_2_SELF_END=2.00
6137
PRIMER_LEFT_2_END_STABILITY=6.7000
6138
PRIMER_RIGHT_2_END_STABILITY=7.3000
6139
PRIMER_PAIR_2_COMPL_ANY=4.00
6140
PRIMER_PAIR_2_COMPL_END=2.00
6141
PRIMER_PRODUCT_SIZE_2=125
6142
PRIMER_PAIR_PENALTY_3=1.6848
6143
PRIMER_LEFT_3_PENALTY=0.396662
6144
PRIMER_RIGHT_3_PENALTY=1.288173
6145
PRIMER_LEFT_3_SEQUENCE=TCAGTCAGGCGAGTGTCTGT
6146
PRIMER_RIGHT_3_SEQUENCE=TCTAGGGGAACTGGCTTTTTC
6147
PRIMER_LEFT_3=208,20
6148
PRIMER_RIGHT_3=334,21
6149
PRIMER_LEFT_3_TM=59.603
6150
PRIMER_RIGHT_3_TM=59.712
6151
PRIMER_LEFT_3_GC_PERCENT=55.000
6152
PRIMER_RIGHT_3_GC_PERCENT=47.619
6153
PRIMER_LEFT_3_SELF_ANY=6.00
6154
PRIMER_RIGHT_3_SELF_ANY=4.00
6155
PRIMER_LEFT_3_SELF_END=4.00
6156
PRIMER_RIGHT_3_SELF_END=2.00
6157
PRIMER_LEFT_3_END_STABILITY=6.4000
6158
PRIMER_RIGHT_3_END_STABILITY=7.3000
6159
PRIMER_PAIR_3_COMPL_ANY=4.00
6160
PRIMER_PAIR_3_COMPL_END=0.00
6161
PRIMER_PRODUCT_SIZE_3=127
6162
PRIMER_PAIR_PENALTY_4=1.9274
6163
PRIMER_LEFT_4_PENALTY=0.178378
6164
PRIMER_RIGHT_4_PENALTY=1.748991
6165
PRIMER_LEFT_4_SEQUENCE=TTCAGTCAGGCGAGTGTCTG
6166
PRIMER_RIGHT_4_SEQUENCE=AGGGGAACTGGCTTTTTCTAA
6167
PRIMER_LEFT_4=207,20
6168
PRIMER_RIGHT_4=331,21
6169
PRIMER_LEFT_4_TM=60.178
6170
PRIMER_RIGHT_4_TM=59.251
6171
PRIMER_LEFT_4_GC_PERCENT=55.000
6172
PRIMER_RIGHT_4_GC_PERCENT=42.857
6173
PRIMER_LEFT_4_SELF_ANY=6.00
6174
PRIMER_RIGHT_4_SELF_ANY=3.00
6175
PRIMER_LEFT_4_SELF_END=6.00
6176
PRIMER_RIGHT_4_SELF_END=2.00
6177
PRIMER_LEFT_4_END_STABILITY=6.4000
6178
PRIMER_RIGHT_4_END_STABILITY=6.0000
6179
PRIMER_PAIR_4_COMPL_ANY=4.00
6180
PRIMER_PAIR_4_COMPL_END=0.00
6181
PRIMER_PRODUCT_SIZE_4=125
6182
=
6183
MARKER_NAME=36.RHAP61FD6.seq
6184
SEQUENCE=GTCTGAGACCCAGCAANATGNGGGACAGAAATAGAAGACCGACCTGTAATTTCATGGACAAGTTAATCTGGAGTTTACAGTACAGCGGAAACAAAAGANNTCCCGCCTCAGCAAAGTGGAAAGGNGAGAACCAANTCCTGAAAATTATCCTCTGATCTCCATTAGTGTTCCATAGCACCATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNACATTNNNNNNNNNNNNNNTGCNANTNTCNCTTNCTNNAAAGANCAACNATGGNNGCACTTTCTCTCTTTAGCNGACCCANNGGNGCGGGTNNTGTNAACTCNNGNTTCTGNGGANNGGGGGTANCGGNGCNCGNNTTNGTTGNNCNTGGTCNTNGCNGTTTCCTTGTTGTGANNTTGTGNNTGCAGCGNNCNGTTCNANNNANNNTGGCGNGCCGGGAGNNTTNAGNGGAAANCCTGNNNGTTGCTTTNTGNNGGNGAGCTANGTCNCNTNNTTTGGGTNNNNNNNNNGGGNCNCTTTT
6185
INCLUDED_REGION=0,530
6186
TARGET=182,46,CA
6187
TARGET=235,14,AC
6188
PRIMER_COMMENT=CL is
6189
PRIMER_COMMENT=CR is
6190
=
6191
MARKER_NAME=38.RHAP61FE5.seq
6192
SEQUENCE=NANACCNCCCCNANCCTGNCCTCTATCTCNNNCCNNNNNNNNNNNNNNNNNNNNTNCNTCCTCTCTCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTGAATTAATTNTAGGTGTGGTAAGCATTTGGTGCCAGCATTAATTTCCAGTCCCACCTTAAGAGNTTACTAGTGCGAANCTGAANTAANATTGACACAGTGAACGACGANTGCTGGGTAAGNGTCNNAGGGGNNCTTTGTNNTCACTCNCTACACTTAGGTGTTAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
6193
INCLUDED_REGION=0,320
6194
TARGET=34,20,CT
6195
TARGET=69,82,AC
6196
PRIMER_COMMENT=CL is
6197
PRIMER_COMMENT=CR is
6198
=
6199
MARKER_NAME=39.RHAP61FE6.seq
6200
SEQUENCE=NANNCNCCTAGAGGACCCTGTCCNGCCAAACATTGTNTTCATGTCATGGACAAGGACAGGGACAGGGACCNTNAAGTTNCTTTTNNCCTACAGGCNCTGCCAATCCTCTGCTTCTGTCATCCCCATAGCATGTCNTAGNGCTGGACATTACACACCNTTCANCGTNNNCNNCNCNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATNNNNNNNNNNNNNNNNNNNNNNNNNNGTNTNNCACAAGCGCCNGCTATCNTGANCCCNNCATAATNNTGGCCGNGTAGTANTCCNNANTGNTTATCTGCTNNCTATGTTTGTANGNNNGANAGNAGGATTNATGNNNCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
6201
INCLUDED_REGION=0,370
6202
TARGET=177,50,GT
6203
TARGET=230,25,TG
6204
PRIMER_COMMENT=CL is
6205
PRIMER_COMMENT=CR is
6206
=
6207
MARKER_NAME=41.RHAP61FF11.seq
6208
SEQUENCE=NNNNNNNNNNNNNNNNNNNNTGTCTGTCAAAACTCCGGTCATCTTCGAGACTTGACACTGGTATTTTTGTGTATGGACCNTTTCCTAGTCACATAGACCTCATTCACAGTGGATANTANTGNGTCTCCCATCAAGTCCCTGAGTCACTGACCTAAGTACAGGCTGTATCATTGACACCATATTTNNTTCTGTTTCTTNNTCGTGGAAGTGNTACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGATGTGNGTGNGNTGTANNGNNNNNATNTNGTANNNNNTNNNNNAGANNNNAAAGGTCAANNNNNAGNNCNNTNTNTGNNAAAAANNNANNNNCNTTANTNTGANANAACTNTCNCNNAGAANGGGNNNCNNNNCNCNAATTCGCTNNNNANGNNAANAGNNNGNNNCNNNNNGNGAAANNNNNNNNCGCNCAAAAAANTNNANGAAAAANANG
6209
INCLUDED_REGION=20,464
6210
TARGET=216,50,GT
6211
PRIMER_COMMENT=CL is
6212
PRIMER_COMMENT=CR is
6213
=
6214
MARKER_NAME=41.RHAP61FF5.seq
6215
SEQUENCE=TNCNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCNNNNNNNNNNNNNNNTGNNATCCCNGGNGGTGACTGTGGGNGCATCNGTNANNGNGGNNGNGTNNNTGTNNCNAANNGTTGTGGGAGTCNNTNNTGTGNCCCTGGTGGNTGNAACTANNTGTAGNTGNTAAGAACACCNGTGGNGAGNTAAGAGNTCTNNCATGGACCGGGNANTNAGNTGGTAANCGNNCGCNAACNCGNNATCANNNNNANANNNNGTTTCCNNGNNNNAAAANNNNNANCCNNNGANNNTNNCNNNANANANTNGNNGNCCGGGGAANANNANGNNNNNANCNTGGGGGGTGNCNNTANGCCGNGAGNNNNNNNNNNANNNNNNTGGGCNNGNNNTCNNNTGNNNNGGNNGTCNANNNNNNNANANGNNTGNNGGNNCNAGNNGNNTNNNGNGAAAGGNNGNNAANNTGCGNGGANANGGNNNTTTGNNTANNGGGAGNATGNT
6216
INCLUDED_REGION=0,494
6217
TARGET=9,26,NG
6218
TARGET=37,14,TG
6219
PRIMER_COMMENT=CL is
6220
PRIMER_COMMENT=CR is
6221
=
6222
MARKER_NAME=42.RHAP61FF12.seq
6223
SEQUENCE=NNNNNNTGTAATCCTGTAATCCAGGGACGTGCTTTTCTGTGTTAGTTGGAAATCTCCAGTAAGTAAAACCATGTGNGNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCTATAACTCCATCCATCCAATATCTAGTATACATNTATATACATTAGAGTTATTTATTACAAAGTACTGATGGACATGATTATNGANGCCAAGAAGTTCCAATTATTTTGAGTGAATTTCNNNAAAAGCCCANACTTANAANAGGAAAATAAAGCCTNTCTCCCTGTNGACAAANTGTCCNTGCGATAANCCTATAANCCCCNAACCCTTTTGGGAACAGTGCCAACCGNNNGGGGGNGGNAAAATTTCATATNNNTGNGGGCCCNNTGGGGNGTCANNTCTGTTNNAAGNNNCAATNNNNAAGGNNANNTTGNNANGGGGTGANCGNGGGNNTGGNNTTCGGNNTTNAANGGGGCNNNAGNTGTNTCCNGGT
6224
INCLUDED_REGION=6,483
6225
TARGET=82,32,GT
6226
PRIMER_COMMENT=CL is CTGTAATCCAGGGACGTGCT
6227
PRIMER_COMMENT=CR is ACTAGATATTGGATGGATGGAGTT
6228
PRIMER_PAIR_PENALTY=6.9582
6229
PRIMER_LEFT_PENALTY=0.133741
6230
PRIMER_RIGHT_PENALTY=6.824436
6231
PRIMER_LEFT_SEQUENCE=CTGTAATCCAGGGACGTGCT
6232
PRIMER_RIGHT_SEQUENCE=ACTAGATATTGGATGGATGGAGTT
6233
PRIMER_LEFT=13,20
6234
PRIMER_RIGHT=143,24
6235
PRIMER_LEFT_TM=60.134
6236
PRIMER_RIGHT_TM=57.176
6237
PRIMER_LEFT_GC_PERCENT=55.000
6238
PRIMER_RIGHT_GC_PERCENT=37.500
6239
PRIMER_LEFT_SELF_ANY=5.00
6240
PRIMER_RIGHT_SELF_ANY=4.00
6241
PRIMER_LEFT_SELF_END=2.00
6242
PRIMER_RIGHT_SELF_END=0.00
6243
PRIMER_LEFT_END_STABILITY=7.9000
6244
PRIMER_RIGHT_END_STABILITY=6.4000
6245
PRIMER_PAIR_COMPL_ANY=5.00
6246
PRIMER_PAIR_COMPL_END=0.00
6247
PRIMER_PRODUCT_SIZE=131
6248
PRIMER_PAIR_PENALTY_1=7.2555
6249
PRIMER_LEFT_1_PENALTY=0.431057
6250
PRIMER_RIGHT_1_PENALTY=6.824436
6251
PRIMER_LEFT_1_SEQUENCE=TAATCCAGGGACGTGCTTTT
6252
PRIMER_RIGHT_1_SEQUENCE=ACTAGATATTGGATGGATGGAGTT
6253
PRIMER_LEFT_1=16,20
6254
PRIMER_RIGHT_1=143,24
6255
PRIMER_LEFT_1_TM=59.569
6256
PRIMER_RIGHT_1_TM=57.176
6257
PRIMER_LEFT_1_GC_PERCENT=45.000
6258
PRIMER_RIGHT_1_GC_PERCENT=37.500
6259
PRIMER_LEFT_1_SELF_ANY=4.00
6260
PRIMER_RIGHT_1_SELF_ANY=4.00
6261
PRIMER_LEFT_1_SELF_END=0.00
6262
PRIMER_RIGHT_1_SELF_END=0.00
6263
PRIMER_LEFT_1_END_STABILITY=7.3000
6264
PRIMER_RIGHT_1_END_STABILITY=6.4000
6265
PRIMER_PAIR_1_COMPL_ANY=5.00
6266
PRIMER_PAIR_1_COMPL_END=0.00
6267
PRIMER_PRODUCT_SIZE_1=128
6268
PRIMER_PAIR_PENALTY_2=7.3411
6269
PRIMER_LEFT_2_PENALTY=0.516683
6270
PRIMER_RIGHT_2_PENALTY=6.824436
6271
PRIMER_LEFT_2_SEQUENCE=TGTAATCCAGGGACGTGCTT
6272
PRIMER_RIGHT_2_SEQUENCE=ACTAGATATTGGATGGATGGAGTT
6273
PRIMER_LEFT_2=14,20
6274
PRIMER_RIGHT_2=143,24
6275
PRIMER_LEFT_2_TM=60.517
6276
PRIMER_RIGHT_2_TM=57.176
6277
PRIMER_LEFT_2_GC_PERCENT=50.000
6278
PRIMER_RIGHT_2_GC_PERCENT=37.500
6279
PRIMER_LEFT_2_SELF_ANY=4.00
6280
PRIMER_RIGHT_2_SELF_ANY=4.00
6281
PRIMER_LEFT_2_SELF_END=4.00
6282
PRIMER_RIGHT_2_SELF_END=0.00
6283
PRIMER_LEFT_2_END_STABILITY=8.5000
6284
PRIMER_RIGHT_2_END_STABILITY=6.4000
6285
PRIMER_PAIR_2_COMPL_ANY=5.00
6286
PRIMER_PAIR_2_COMPL_END=0.00
6287
PRIMER_PRODUCT_SIZE_2=130
6288
PRIMER_PAIR_PENALTY_3=7.5683
6289
PRIMER_LEFT_3_PENALTY=0.133741
6290
PRIMER_RIGHT_3_PENALTY=7.434573
6291
PRIMER_LEFT_3_SEQUENCE=CTGTAATCCAGGGACGTGCT
6292
PRIMER_RIGHT_3_SEQUENCE=GATATTGGATGGATGGAGTTATAGA
6293
PRIMER_LEFT_3=13,20
6294
PRIMER_RIGHT_3=139,25
6295
PRIMER_LEFT_3_TM=60.134
6296
PRIMER_RIGHT_3_TM=57.565
6297
PRIMER_LEFT_3_GC_PERCENT=55.000
6298
PRIMER_RIGHT_3_GC_PERCENT=36.000
6299
PRIMER_LEFT_3_SELF_ANY=5.00
6300
PRIMER_RIGHT_3_SELF_ANY=4.00
6301
PRIMER_LEFT_3_SELF_END=2.00
6302
PRIMER_RIGHT_3_SELF_END=2.00
6303
PRIMER_LEFT_3_END_STABILITY=7.9000
6304
PRIMER_RIGHT_3_END_STABILITY=5.6000
6305
PRIMER_PAIR_3_COMPL_ANY=6.00
6306
PRIMER_PAIR_3_COMPL_END=1.00
6307
PRIMER_PRODUCT_SIZE_3=127
6308
PRIMER_PAIR_PENALTY_4=7.7371
6309
PRIMER_LEFT_4_PENALTY=0.912701
6310
PRIMER_RIGHT_4_PENALTY=6.824436
6311
PRIMER_LEFT_4_SEQUENCE=TCCTGTAATCCAGGGACGTG
6312
PRIMER_RIGHT_4_SEQUENCE=ACTAGATATTGGATGGATGGAGTT
6313
PRIMER_LEFT_4=11,20
6314
PRIMER_RIGHT_4=143,24
6315
PRIMER_LEFT_4_TM=60.913
6316
PRIMER_RIGHT_4_TM=57.176
6317
PRIMER_LEFT_4_GC_PERCENT=55.000
6318
PRIMER_RIGHT_4_GC_PERCENT=37.500
6319
PRIMER_LEFT_4_SELF_ANY=7.00
6320
PRIMER_RIGHT_4_SELF_ANY=4.00
6321
PRIMER_LEFT_4_SELF_END=2.00
6322
PRIMER_RIGHT_4_SELF_END=0.00
6323
PRIMER_LEFT_4_END_STABILITY=8.1000
6324
PRIMER_RIGHT_4_END_STABILITY=6.4000
6325
PRIMER_PAIR_4_COMPL_ANY=5.00
6326
PRIMER_PAIR_4_COMPL_END=0.00
6327
PRIMER_PRODUCT_SIZE_4=133
6328
=
6329
MARKER_NAME=44.RHAP61FG5.seq
6330
SEQUENCE=NNNNNNNNNNTATCTCTGACTTTGTTGTTTTGNTTTTCCGCANTTTTCATTGTGAATTCNATTTTTGNTTGNCTTTNATTTGCTGACNATCTCTTTATTAAANTTNANGTCTTGAATTGNTTCANTTATTTCACCCAACNANTCTNNNGGGGTANTCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACGCNTATGGGGGTGNNTTTTACAGTCTCCCNNNAGANATNNATGGTCTATNNTCNNTAAGANCNNTGGACATANTCATAANNACNANTCAGTTAACANNGCNCCNNNAAATTAGNNNNTCNATNNANAATTGNNAGTGGNNAGTCCNTGGCCNGGGCCTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
6331
INCLUDED_REGION=10,395
6332
TARGET=159,84,GT
6333
PRIMER_COMMENT=CL is
6334
PRIMER_COMMENT=CR is
6335
=
6336
MARKER_NAME=47.RHAP61FH11.seq
6337
SEQUENCE=NNNNNNNNNNNNNNNNNNNTGTCAAAATTAAAAACCAGCAAGAAAGGTCACTTNNATTTTAAGTGGAGAGAGGAAATAATTNANGGATAAAATATAAAACAATAATGAAAAATTAATTTCATCATGGACTGCNTATTAAGANNNANNTGTACATAAGNGNTATTGTTGTGTCANNAGTTAACTTCCCNGNTANCCCGNACAGGNNNTNGGGTCTANNANNCNNNANGGCCNACNTNAANNTTAAATGNCANNTTGTTTTCNGGNGTGGNTTCAATGCNCTNTGNNNTNNNNCNCNNNGNNNNNNNNNNNNNNNNNNNNTGNTGNNNNNNNNNNNNNNNNNGNNNANAANNGGTNTNAANNANTTANGNTNNNNNGNNATNNGNGNNGNTNGTGTGNNNNNNGGTNNTGNTNGNGTGNTNCNNTGTTGNTNNNNNNNNNNTNNNNGANNGNATNNTGNCNAANNNNNNCGNNNNNNNNNNCTTNTTGNANNGNTNNGNATTGNNNNTTT
6338
INCLUDED_REGION=19,489
6339
TARGET=300,18,NG
6340
TARGET=323,16,GT
6341
PRIMER_COMMENT=CL is
6342
PRIMER_COMMENT=CR is
6343
=
6344
MARKER_NAME=48.RHAP61FH6.seq
6345
SEQUENCE=NNNNNNNNNNTTGTGTCANACTNACAAAAATCNAACCAGGACACAATGTTNANCTTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAAGTTTATGTCCTTTTCATCTCTTGTAGCATTTAGTATGCACTGAGGCCATATCTTTTGAAGTGAGAGTTATACTGACATATTAGTATAAATTTCTTTTTGCATCTCTGNAGTTTTATAGGATTGTGTGTANTGTAGAATCTAACAAACTTTANTAAGTCTAGCATCTCACCTAAAGGAAAGNNCTCTGGGGGCCTACACTGGGGTGTGACCAAACTGCCTGGCACCAGTAGGCGCTCTCCATGGGGGGNCATTCTTCAGGTACAATAAGGGGTTGTCTGTTCAATACAGCNGTGGGGGGAACNTGCTGGCTGATAAGACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
6346
INCLUDED_REGION=10,433
6347
TARGET=58,62,AC
6348
PRIMER_COMMENT=CL is
6349
PRIMER_COMMENT=CR is
6350
=
6351
MARKER_NAME=01.RHAP64FA1.seq
6352
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNCAACGGGNTGGCTCAGTGGNTAGAGGTACTTGCCCACCNAGTTTTAACAACTGGAGTTCGGATCCCAGGGTCCCACGTCATGGAAGACAGGGAAAGNNTCCCACANTGGTTTACACACACTNCNTCGCACACAGTCACGCGCANGCGNAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGGAGCACCAGAGGACGACAGAGGACAACAGAGAGCTNNNCNGTCACAGNCTCCNGACNNNNNNGTAATTAAGANNTTTGAGNAGNNNCNNNNGTGTTNGNTGNCAAAGAANAGNGNGGNNNTTNNNNNGNNAGTGGTGNANAGGNANNTTGGNCNGGNANGGAGGGCNNNNCCNTGGNGGNNGGNTNNNAAGNNNNNTGCNGNNNGANNGGNGNNNCGTGGNTGGGNNNGNANACNAGNNNNNNNANNNNNNTTGNNNNNGNNNTNNNNANCCNGGNGTNNNGAGNANNNGTTNNNNNNGAGNGNNNGTNNNC
6353
INCLUDED_REGION=25,495
6354
TARGET=176,28,GA
6355
PRIMER_COMMENT=CL is ACGTCATGGAAGACAGGGAA
6356
PRIMER_COMMENT=CR is CTCTGTCGTCCTCTGGTGCT
6357
PRIMER_PAIR_PENALTY=1.1184
6358
PRIMER_LEFT_PENALTY=0.510331
6359
PRIMER_RIGHT_PENALTY=0.608084
6360
PRIMER_LEFT_SEQUENCE=ACGTCATGGAAGACAGGGAA
6361
PRIMER_RIGHT_SEQUENCE=CTCTGTCGTCCTCTGGTGCT
6362
PRIMER_LEFT=99,20
6363
PRIMER_RIGHT=228,20
6364
PRIMER_LEFT_TM=60.510
6365
PRIMER_RIGHT_TM=60.608
6366
PRIMER_LEFT_GC_PERCENT=50.000
6367
PRIMER_RIGHT_GC_PERCENT=60.000
6368
PRIMER_LEFT_SELF_ANY=4.00
6369
PRIMER_RIGHT_SELF_ANY=2.00
6370
PRIMER_LEFT_SELF_END=0.00
6371
PRIMER_RIGHT_SELF_END=0.00
6372
PRIMER_LEFT_END_STABILITY=9.7000
6373
PRIMER_RIGHT_END_STABILITY=7.9000
6374
PRIMER_PAIR_COMPL_ANY=7.00
6375
PRIMER_PAIR_COMPL_END=4.00
6376
PRIMER_PRODUCT_SIZE=130
6377
PRIMER_PAIR_PENALTY_1=1.2362
6378
PRIMER_LEFT_1_PENALTY=0.510331
6379
PRIMER_RIGHT_1_PENALTY=0.725890
6380
PRIMER_LEFT_1_SEQUENCE=ACGTCATGGAAGACAGGGAA
6381
PRIMER_RIGHT_1_SEQUENCE=GTCCTCTGTCGTCCTCTGGT
6382
PRIMER_LEFT_1=99,20
6383
PRIMER_RIGHT_1=231,20
6384
PRIMER_LEFT_1_TM=60.510
6385
PRIMER_RIGHT_1_TM=59.274
6386
PRIMER_LEFT_1_GC_PERCENT=50.000
6387
PRIMER_RIGHT_1_GC_PERCENT=60.000
6388
PRIMER_LEFT_1_SELF_ANY=4.00
6389
PRIMER_RIGHT_1_SELF_ANY=2.00
6390
PRIMER_LEFT_1_SELF_END=0.00
6391
PRIMER_RIGHT_1_SELF_END=0.00
6392
PRIMER_LEFT_1_END_STABILITY=9.7000
6393
PRIMER_RIGHT_1_END_STABILITY=7.9000
6394
PRIMER_PAIR_1_COMPL_ANY=8.00
6395
PRIMER_PAIR_1_COMPL_END=5.00
6396
PRIMER_PRODUCT_SIZE_1=133
6397
PRIMER_PAIR_PENALTY_2=1.5461
6398
PRIMER_LEFT_2_PENALTY=0.510331
6399
PRIMER_RIGHT_2_PENALTY=1.035741
6400
PRIMER_LEFT_2_SEQUENCE=ACGTCATGGAAGACAGGGAA
6401
PRIMER_RIGHT_2_SEQUENCE=TTGTCCTCTGTCGTCCTCTG
6402
PRIMER_LEFT_2=99,20
6403
PRIMER_RIGHT_2=233,20
6404
PRIMER_LEFT_2_TM=60.510
6405
PRIMER_RIGHT_2_TM=58.964
6406
PRIMER_LEFT_2_GC_PERCENT=50.000
6407
PRIMER_RIGHT_2_GC_PERCENT=55.000
6408
PRIMER_LEFT_2_SELF_ANY=4.00
6409
PRIMER_RIGHT_2_SELF_ANY=2.00
6410
PRIMER_LEFT_2_SELF_END=0.00
6411
PRIMER_RIGHT_2_SELF_END=1.00
6412
PRIMER_LEFT_2_END_STABILITY=9.7000
6413
PRIMER_RIGHT_2_END_STABILITY=6.7000
6414
PRIMER_PAIR_2_COMPL_ANY=8.00
6415
PRIMER_PAIR_2_COMPL_END=6.00
6416
PRIMER_PRODUCT_SIZE_2=135
6417
PRIMER_PAIR_PENALTY_3=1.6160
6418
PRIMER_LEFT_3_PENALTY=1.007886
6419
PRIMER_RIGHT_3_PENALTY=0.608084
6420
PRIMER_LEFT_3_SEQUENCE=AACAACTGGAGTTCGGATCC
6421
PRIMER_RIGHT_3_SEQUENCE=CTCTGTCGTCCTCTGGTGCT
6422
PRIMER_LEFT_3=70,20
6423
PRIMER_RIGHT_3=228,20
6424
PRIMER_LEFT_3_TM=58.992
6425
PRIMER_RIGHT_3_TM=60.608
6426
PRIMER_LEFT_3_GC_PERCENT=50.000
6427
PRIMER_RIGHT_3_GC_PERCENT=60.000
6428
PRIMER_LEFT_3_SELF_ANY=6.00
6429
PRIMER_RIGHT_3_SELF_ANY=2.00
6430
PRIMER_LEFT_3_SELF_END=6.00
6431
PRIMER_RIGHT_3_SELF_END=0.00
6432
PRIMER_LEFT_3_END_STABILITY=7.8000
6433
PRIMER_RIGHT_3_END_STABILITY=7.9000
6434
PRIMER_PAIR_3_COMPL_ANY=3.00
6435
PRIMER_PAIR_3_COMPL_END=0.00
6436
PRIMER_PRODUCT_SIZE_3=159
6437
PRIMER_PAIR_PENALTY_4=1.6186
6438
PRIMER_LEFT_4_PENALTY=0.510331
6439
PRIMER_RIGHT_4_PENALTY=1.108240
6440
PRIMER_LEFT_4_SEQUENCE=ACGTCATGGAAGACAGGGAA
6441
PRIMER_RIGHT_4_SEQUENCE=TGTTGTCCTCTGTCGTCCTCT
6442
PRIMER_LEFT_4=99,20
6443
PRIMER_RIGHT_4=235,21
6444
PRIMER_LEFT_4_TM=60.510
6445
PRIMER_RIGHT_4_TM=59.892
6446
PRIMER_LEFT_4_GC_PERCENT=50.000
6447
PRIMER_RIGHT_4_GC_PERCENT=52.381
6448
PRIMER_LEFT_4_SELF_ANY=4.00
6449
PRIMER_RIGHT_4_SELF_ANY=2.00
6450
PRIMER_LEFT_4_SELF_END=0.00
6451
PRIMER_RIGHT_4_SELF_END=0.00
6452
PRIMER_LEFT_4_END_STABILITY=9.7000
6453
PRIMER_RIGHT_4_END_STABILITY=7.9000
6454
PRIMER_PAIR_4_COMPL_ANY=8.00
6455
PRIMER_PAIR_4_COMPL_END=5.00
6456
PRIMER_PRODUCT_SIZE_4=137
6457
=
6458
MARKER_NAME=01.RHAP64FA7.seq
6459
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNCCACANAAAACATCCTGTCNNNNNNNNNNNNNNNNTAGCTNNNNNNNNNNNNNNNNTATAAGTGCATGGAATGAGTGTGTATANNNNNNNNNNNNNNNNNNNNNNNNTTAGCTTANNNNNNNNNNNNNNNNNNNNNNNNTATAAGTGCATGGAAGGAGTGTGTGTATGTGACTGTGTGCTGAATATGTAAGCGTGNGGCGGTAAANNNNNNNNNNNNNNNNNNNNNNTAANTGNNNGACNNAAGCCNCAANGNGCGCTNTGTGCACTGTGTCNATNCGCCCGNGTCANCACNGCCACNCANNCANAACANGCCNNNAGCGNCGTGCATGCTGANCCGNCCCATATCNGNGNNNACCTANNNNACAACNNCANNNNCCGAAAGCNNNCGCCATCCCCNTANACANGNCCCCNNCNGCCACTNCNNCANCNNNGANNCACNTCTGANCCNNGCNCATNTCNNANNNANGACCNCTNNNTCCCGNC
6460
INCLUDED_REGION=22,483
6461
TARGET=43,14,GT
6462
TARGET=62,16,TG
6463
TARGET=105,24,TG
6464
TARGET=137,24,TG
6465
TARGET=227,22,TG
6466
PRIMER_COMMENT=CL is AAGTGCATGGAATGAGTGTGT
6467
PRIMER_COMMENT=CR is CGCTTACATATTCAGCACACAG
6468
PRIMER_PAIR_PENALTY=6.3873
6469
PRIMER_LEFT_PENALTY=2.927648
6470
PRIMER_RIGHT_PENALTY=3.459697
6471
PRIMER_LEFT_SEQUENCE=AAGTGCATGGAATGAGTGTGT
6472
PRIMER_RIGHT_SEQUENCE=CGCTTACATATTCAGCACACAG
6473
PRIMER_LEFT=81,21
6474
PRIMER_RIGHT=214,22
6475
PRIMER_LEFT_TM=58.072
6476
PRIMER_RIGHT_TM=58.540
6477
PRIMER_LEFT_GC_PERCENT=42.857
6478
PRIMER_RIGHT_GC_PERCENT=45.455
6479
PRIMER_LEFT_SELF_ANY=4.00
6480
PRIMER_RIGHT_SELF_ANY=6.00
6481
PRIMER_LEFT_SELF_END=0.00
6482
PRIMER_RIGHT_SELF_END=1.00
6483
PRIMER_LEFT_END_STABILITY=6.4000
6484
PRIMER_RIGHT_END_STABILITY=6.7000
6485
PRIMER_PAIR_COMPL_ANY=5.00
6486
PRIMER_PAIR_COMPL_END=4.00
6487
PRIMER_PRODUCT_SIZE=134
6488
PRIMER_PAIR_PENALTY_1=6.3931
6489
PRIMER_LEFT_1_PENALTY=2.933397
6490
PRIMER_RIGHT_1_PENALTY=3.459697
6491
PRIMER_LEFT_1_SEQUENCE=AAGTGCATGGAATGAGTGTG
6492
PRIMER_RIGHT_1_SEQUENCE=CGCTTACATATTCAGCACACAG
6493
PRIMER_LEFT_1=81,20
6494
PRIMER_RIGHT_1=214,22
6495
PRIMER_LEFT_1_TM=57.067
6496
PRIMER_RIGHT_1_TM=58.540
6497
PRIMER_LEFT_1_GC_PERCENT=45.000
6498
PRIMER_RIGHT_1_GC_PERCENT=45.455
6499
PRIMER_LEFT_1_SELF_ANY=4.00
6500
PRIMER_RIGHT_1_SELF_ANY=6.00
6501
PRIMER_LEFT_1_SELF_END=0.00
6502
PRIMER_RIGHT_1_SELF_END=1.00
6503
PRIMER_LEFT_1_END_STABILITY=6.4000
6504
PRIMER_RIGHT_1_END_STABILITY=6.7000
6505
PRIMER_PAIR_1_COMPL_ANY=5.00
6506
PRIMER_PAIR_1_COMPL_END=3.00
6507
PRIMER_PRODUCT_SIZE_1=134
6508
PRIMER_PAIR_PENALTY_2=6.4394
6509
PRIMER_LEFT_2_PENALTY=2.927648
6510
PRIMER_RIGHT_2_PENALTY=3.511795
6511
PRIMER_LEFT_2_SEQUENCE=AAGTGCATGGAATGAGTGTGT
6512
PRIMER_RIGHT_2_SEQUENCE=CGCTTACATATTCAGCACACA
6513
PRIMER_LEFT_2=81,21
6514
PRIMER_RIGHT_2=214,21
6515
PRIMER_LEFT_2_TM=58.072
6516
PRIMER_RIGHT_2_TM=57.488
6517
PRIMER_LEFT_2_GC_PERCENT=42.857
6518
PRIMER_RIGHT_2_GC_PERCENT=42.857
6519
PRIMER_LEFT_2_SELF_ANY=4.00
6520
PRIMER_RIGHT_2_SELF_ANY=6.00
6521
PRIMER_LEFT_2_SELF_END=0.00
6522
PRIMER_RIGHT_2_SELF_END=0.00
6523
PRIMER_LEFT_2_END_STABILITY=6.4000
6524
PRIMER_RIGHT_2_END_STABILITY=6.4000
6525
PRIMER_PAIR_2_COMPL_ANY=5.00
6526
PRIMER_PAIR_2_COMPL_END=5.00
6527
PRIMER_PRODUCT_SIZE_2=134
6528
PRIMER_PAIR_PENALTY_3=6.4394
6529
PRIMER_LEFT_3_PENALTY=2.927648
6530
PRIMER_RIGHT_3_PENALTY=3.511795
6531
PRIMER_LEFT_3_SEQUENCE=AAGTGCATGGAATGAGTGTGT
6532
PRIMER_RIGHT_3_SEQUENCE=CACGCTTACATATTCAGCACA
6533
PRIMER_LEFT_3=81,21
6534
PRIMER_RIGHT_3=216,21
6535
PRIMER_LEFT_3_TM=58.072
6536
PRIMER_RIGHT_3_TM=57.488
6537
PRIMER_LEFT_3_GC_PERCENT=42.857
6538
PRIMER_RIGHT_3_GC_PERCENT=42.857
6539
PRIMER_LEFT_3_SELF_ANY=4.00
6540
PRIMER_RIGHT_3_SELF_ANY=6.00
6541
PRIMER_LEFT_3_SELF_END=0.00
6542
PRIMER_RIGHT_3_SELF_END=1.00
6543
PRIMER_LEFT_3_END_STABILITY=6.4000
6544
PRIMER_RIGHT_3_END_STABILITY=8.2000
6545
PRIMER_PAIR_3_COMPL_ANY=5.00
6546
PRIMER_PAIR_3_COMPL_END=4.00
6547
PRIMER_PRODUCT_SIZE_3=136
6548
PRIMER_PAIR_PENALTY_4=6.4452
6549
PRIMER_LEFT_4_PENALTY=2.933397
6550
PRIMER_RIGHT_4_PENALTY=3.511795
6551
PRIMER_LEFT_4_SEQUENCE=AAGTGCATGGAATGAGTGTG
6552
PRIMER_RIGHT_4_SEQUENCE=CGCTTACATATTCAGCACACA
6553
PRIMER_LEFT_4=81,20
6554
PRIMER_RIGHT_4=214,21
6555
PRIMER_LEFT_4_TM=57.067
6556
PRIMER_RIGHT_4_TM=57.488
6557
PRIMER_LEFT_4_GC_PERCENT=45.000
6558
PRIMER_RIGHT_4_GC_PERCENT=42.857
6559
PRIMER_LEFT_4_SELF_ANY=4.00
6560
PRIMER_RIGHT_4_SELF_ANY=6.00
6561
PRIMER_LEFT_4_SELF_END=0.00
6562
PRIMER_RIGHT_4_SELF_END=0.00
6563
PRIMER_LEFT_4_END_STABILITY=6.4000
6564
PRIMER_RIGHT_4_END_STABILITY=6.4000
6565
PRIMER_PAIR_4_COMPL_ANY=5.00
6566
PRIMER_PAIR_4_COMPL_END=4.00
6567
PRIMER_PRODUCT_SIZE_4=134
6568
=
6569
MARKER_NAME=02.RHAP64FA8.seq
6570
SEQUENCE=NNNNNNNNNNNNCCANTGACANAGCCTCTCTTGAAAATGAGGTTGAGGACCTGCCTCAGGAACAGGAGCCAGGGTCANTTGTAGCCTCTACACTCTTTTGCANTGAGGTGCAGGTCATATAGATGCAAGGGGCAAAAGGGGATCGGCACGTGCGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGACAAANACCCAGATTCATANAAAANACAGTNANGTAGGAACAGGNNCACACATACTTANACANNNGTAAACGTGGAAAAATGGCANATNNNNNNNNNNNNNNNNNNNNNNNNNNCCAGGGNNNNACACACCACACAAAAGANGTGGTNNNNACATNCNAACATNNACNNNAACNANNGTGNNAANTCACNNANTNNTNCNTNTNNTGGNAANNANNCANGNAAAAGTGTNNACACACTTANANNNAGGCAAATNTGTNNGNA
6571
INCLUDED_REGION=12,459
6572
TARGET=156,50,CA
6573
TARGET=297,26,CA
6574
PRIMER_COMMENT=CL is TAGATGCAAGGGGCAAAAG
6575
PRIMER_COMMENT=CR is TGCCATTTTTCCACGTTTAC
6576
PRIMER_PAIR_PENALTY=3.6129
6577
PRIMER_LEFT_PENALTY=2.144984
6578
PRIMER_RIGHT_PENALTY=1.467885
6579
PRIMER_LEFT_SEQUENCE=TAGATGCAAGGGGCAAAAG
6580
PRIMER_RIGHT_SEQUENCE=TGCCATTTTTCCACGTTTAC
6581
PRIMER_LEFT=119,19
6582
PRIMER_RIGHT=293,20
6583
PRIMER_LEFT_TM=58.855
6584
PRIMER_RIGHT_TM=58.532
6585
PRIMER_LEFT_GC_PERCENT=47.368
6586
PRIMER_RIGHT_GC_PERCENT=40.000
6587
PRIMER_LEFT_SELF_ANY=4.00
6588
PRIMER_RIGHT_SELF_ANY=4.00
6589
PRIMER_LEFT_SELF_END=0.00
6590
PRIMER_RIGHT_SELF_END=2.00
6591
PRIMER_LEFT_END_STABILITY=7.3000
6592
PRIMER_RIGHT_END_STABILITY=6.0000
6593
PRIMER_PAIR_COMPL_ANY=5.00
6594
PRIMER_PAIR_COMPL_END=3.00
6595
PRIMER_PRODUCT_SIZE=175
6596
PRIMER_PAIR_PENALTY_1=3.8024
6597
PRIMER_LEFT_1_PENALTY=2.334562
6598
PRIMER_RIGHT_1_PENALTY=1.467885
6599
PRIMER_LEFT_1_SEQUENCE=TAGATGCAAGGGGCAAAAGG
6600
PRIMER_RIGHT_1_SEQUENCE=TGCCATTTTTCCACGTTTAC
6601
PRIMER_LEFT_1=119,20
6602
PRIMER_RIGHT_1=293,20
6603
PRIMER_LEFT_1_TM=62.335
6604
PRIMER_RIGHT_1_TM=58.532
6605
PRIMER_LEFT_1_GC_PERCENT=50.000
6606
PRIMER_RIGHT_1_GC_PERCENT=40.000
6607
PRIMER_LEFT_1_SELF_ANY=4.00
6608
PRIMER_RIGHT_1_SELF_ANY=4.00
6609
PRIMER_LEFT_1_SELF_END=0.00
6610
PRIMER_RIGHT_1_SELF_END=2.00
6611
PRIMER_LEFT_1_END_STABILITY=8.5000
6612
PRIMER_RIGHT_1_END_STABILITY=6.0000
6613
PRIMER_PAIR_1_COMPL_ANY=5.00
6614
PRIMER_PAIR_1_COMPL_END=3.00
6615
PRIMER_PRODUCT_SIZE_1=175
6616
PRIMER_PAIR_PENALTY_2=4.6324
6617
PRIMER_LEFT_2_PENALTY=3.164492
6618
PRIMER_RIGHT_2_PENALTY=1.467885
6619
PRIMER_LEFT_2_SEQUENCE=AAGGGGCAAAAGGGGATC
6620
PRIMER_RIGHT_2_SEQUENCE=TGCCATTTTTCCACGTTTAC
6621
PRIMER_LEFT_2=126,18
6622
PRIMER_RIGHT_2=293,20
6623
PRIMER_LEFT_2_TM=61.164
6624
PRIMER_RIGHT_2_TM=58.532
6625
PRIMER_LEFT_2_GC_PERCENT=55.556
6626
PRIMER_RIGHT_2_GC_PERCENT=40.000
6627
PRIMER_LEFT_2_SELF_ANY=4.00
6628
PRIMER_RIGHT_2_SELF_ANY=4.00
6629
PRIMER_LEFT_2_SELF_END=4.00
6630
PRIMER_RIGHT_2_SELF_END=2.00
6631
PRIMER_LEFT_2_END_STABILITY=7.8000
6632
PRIMER_RIGHT_2_END_STABILITY=6.0000
6633
PRIMER_PAIR_2_COMPL_ANY=6.00
6634
PRIMER_PAIR_2_COMPL_END=3.00
6635
PRIMER_PRODUCT_SIZE_2=168
6636
PRIMER_PAIR_PENALTY_3=4.6710
6637
PRIMER_LEFT_3_PENALTY=3.203163
6638
PRIMER_RIGHT_3_PENALTY=1.467885
6639
PRIMER_LEFT_3_SEQUENCE=AGATGCAAGGGGCAAAAG
6640
PRIMER_RIGHT_3_SEQUENCE=TGCCATTTTTCCACGTTTAC
6641
PRIMER_LEFT_3=120,18
6642
PRIMER_RIGHT_3=293,20
6643
PRIMER_LEFT_3_TM=58.797
6644
PRIMER_RIGHT_3_TM=58.532
6645
PRIMER_LEFT_3_GC_PERCENT=50.000
6646
PRIMER_RIGHT_3_GC_PERCENT=40.000
6647
PRIMER_LEFT_3_SELF_ANY=4.00
6648
PRIMER_RIGHT_3_SELF_ANY=4.00
6649
PRIMER_LEFT_3_SELF_END=0.00
6650
PRIMER_RIGHT_3_SELF_END=2.00
6651
PRIMER_LEFT_3_END_STABILITY=7.3000
6652
PRIMER_RIGHT_3_END_STABILITY=6.0000
6653
PRIMER_PAIR_3_COMPL_ANY=5.00
6654
PRIMER_PAIR_3_COMPL_END=3.00
6655
PRIMER_PRODUCT_SIZE_3=174
6656
PRIMER_PAIR_PENALTY_4=4.8810
6657
PRIMER_LEFT_4_PENALTY=3.413156
6658
PRIMER_RIGHT_4_PENALTY=1.467885
6659
PRIMER_LEFT_4_SEQUENCE=AGATGCAAGGGGCAAAAGG
6660
PRIMER_RIGHT_4_SEQUENCE=TGCCATTTTTCCACGTTTAC
6661
PRIMER_LEFT_4=120,19
6662
PRIMER_RIGHT_4=293,20
6663
PRIMER_LEFT_4_TM=62.413
6664
PRIMER_RIGHT_4_TM=58.532
6665
PRIMER_LEFT_4_GC_PERCENT=52.632
6666
PRIMER_RIGHT_4_GC_PERCENT=40.000
6667
PRIMER_LEFT_4_SELF_ANY=4.00
6668
PRIMER_RIGHT_4_SELF_ANY=4.00
6669
PRIMER_LEFT_4_SELF_END=0.00
6670
PRIMER_RIGHT_4_SELF_END=2.00
6671
PRIMER_LEFT_4_END_STABILITY=8.5000
6672
PRIMER_RIGHT_4_END_STABILITY=6.0000
6673
PRIMER_PAIR_4_COMPL_ANY=5.00
6674
PRIMER_PAIR_4_COMPL_END=3.00
6675
PRIMER_PRODUCT_SIZE_4=174
6676
=
6677
MARKER_NAME=03.RHAP64FA3.seq
6678
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCCTGGAACTGACAAAGTAACCCCAATGTTTGTTGGTAACGTCTTTAGTTCCATGAAACTATCACAGGCTTCAGAGTGCTGTTCTGCCCTGTTCACACTACAAATTCAATTTGATTCTCTTCCGTCTCTAAAGTTCTAATGTAAACTGTACAGCTGACAAAGCATAAATAAGGCTTTAAATGCTAAGGGTTCGCTGTAACAGGATGTAACTGTAATTTACTCTCAAATATATCTTTTAAATCTCCACGTGTGTCAGAAAGAGACAAAGACATGCATGCTTGTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAAGAGGGAGAGGGNNAGGGNGNAGGAAATTGNNAAAATGAAA
6679
INCLUDED_REGION=21,423
6680
TARGET=304,34,AC
6681
TARGET=346,54,GA
6682
PRIMER_COMMENT=CL is
6683
PRIMER_COMMENT=CR is
6684
=
6685
MARKER_NAME=04.RHAP64FB7.seq
6686
SEQUENCE=NNNNNNNNNNNNCCTCCACTGCAATCAAAAGATGANCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTTCTCTTATAAGACTTAGGATATTAAAACAAATGAGAGAAATGGACTCTAGGGTATCCAATAGTAAGTCAACAAGGGTTGGTGAGTTCCTGTCCTGTAACAGACCCAAGAGCCACCCACGTCTAATCTGNAGGGANACAAACAGGGGGGCAATAAGGGAATGGGAGAGAGGAAGANAGTCTCATGTCACGCAGAAACGNTCTCCCCGAGCATCNCAAGTGGAGACCTGGGAACAGATTCAGGTAGATTGTAGGAATACAGGGGANATGCCCTAGGNAGCAGCAGGTAGCTGGAGAGAATCNAAGGCTTCTGGGAGCTGATGTGAGAGCATGCACNGAGAAGNNTCCTGCATCTGTCTGTGTCTNAGTTAGTGGTTTCACNNC
6687
INCLUDED_REGION=12,455
6688
TARGET=39,44,AC
6689
PRIMER_COMMENT=CL is CCTCCACTGCAATCAAAAGA
6690
PRIMER_COMMENT=CR is CAGGAACTCACCAACCCTTG
6691
PRIMER_PAIR_PENALTY=1.6881
6692
PRIMER_LEFT_PENALTY=1.152564
6693
PRIMER_RIGHT_PENALTY=0.535575
6694
PRIMER_LEFT_SEQUENCE=CCTCCACTGCAATCAAAAGA
6695
PRIMER_RIGHT_SEQUENCE=CAGGAACTCACCAACCCTTG
6696
PRIMER_LEFT=12,20
6697
PRIMER_RIGHT=175,20
6698
PRIMER_LEFT_TM=58.847
6699
PRIMER_RIGHT_TM=60.536
6700
PRIMER_LEFT_GC_PERCENT=45.000
6701
PRIMER_RIGHT_GC_PERCENT=55.000
6702
PRIMER_LEFT_SELF_ANY=4.00
6703
PRIMER_RIGHT_SELF_ANY=3.00
6704
PRIMER_LEFT_SELF_END=0.00
6705
PRIMER_RIGHT_SELF_END=3.00
6706
PRIMER_LEFT_END_STABILITY=7.0000
6707
PRIMER_RIGHT_END_STABILITY=8.5000
6708
PRIMER_PAIR_COMPL_ANY=3.00
6709
PRIMER_PAIR_COMPL_END=1.00
6710
PRIMER_PRODUCT_SIZE=164
6711
PRIMER_PAIR_PENALTY_1=2.1523
6712
PRIMER_LEFT_1_PENALTY=1.152564
6713
PRIMER_RIGHT_1_PENALTY=0.999698
6714
PRIMER_LEFT_1_SEQUENCE=CCTCCACTGCAATCAAAAGA
6715
PRIMER_RIGHT_1_SEQUENCE=AGGACAGGAACTCACCAACC
6716
PRIMER_LEFT_1=12,20
6717
PRIMER_RIGHT_1=179,20
6718
PRIMER_LEFT_1_TM=58.847
6719
PRIMER_RIGHT_1_TM=59.000
6720
PRIMER_LEFT_1_GC_PERCENT=45.000
6721
PRIMER_RIGHT_1_GC_PERCENT=55.000
6722
PRIMER_LEFT_1_SELF_ANY=4.00
6723
PRIMER_RIGHT_1_SELF_ANY=2.00
6724
PRIMER_LEFT_1_SELF_END=0.00
6725
PRIMER_RIGHT_1_SELF_END=0.00
6726
PRIMER_LEFT_1_END_STABILITY=7.0000
6727
PRIMER_RIGHT_1_END_STABILITY=8.2000
6728
PRIMER_PAIR_1_COMPL_ANY=3.00
6729
PRIMER_PAIR_1_COMPL_END=0.00
6730
PRIMER_PRODUCT_SIZE_1=168
6731
PRIMER_PAIR_PENALTY_2=2.1523
6732
PRIMER_LEFT_2_PENALTY=1.152564
6733
PRIMER_RIGHT_2_PENALTY=0.999698
6734
PRIMER_LEFT_2_SEQUENCE=CCTCCACTGCAATCAAAAGA
6735
PRIMER_RIGHT_2_SEQUENCE=GACAGGAACTCACCAACCCT
6736
PRIMER_LEFT_2=12,20
6737
PRIMER_RIGHT_2=177,20
6738
PRIMER_LEFT_2_TM=58.847
6739
PRIMER_RIGHT_2_TM=59.000
6740
PRIMER_LEFT_2_GC_PERCENT=45.000
6741
PRIMER_RIGHT_2_GC_PERCENT=55.000
6742
PRIMER_LEFT_2_SELF_ANY=4.00
6743
PRIMER_RIGHT_2_SELF_ANY=3.00
6744
PRIMER_LEFT_2_SELF_END=0.00
6745
PRIMER_RIGHT_2_SELF_END=0.00
6746
PRIMER_LEFT_2_END_STABILITY=7.0000
6747
PRIMER_RIGHT_2_END_STABILITY=9.1000
6748
PRIMER_PAIR_2_COMPL_ANY=3.00
6749
PRIMER_PAIR_2_COMPL_END=1.00
6750
PRIMER_PRODUCT_SIZE_2=166
6751
PRIMER_PAIR_PENALTY_3=2.2611
6752
PRIMER_LEFT_3_PENALTY=1.152564
6753
PRIMER_RIGHT_3_PENALTY=1.108512
6754
PRIMER_LEFT_3_SEQUENCE=CCTCCACTGCAATCAAAAGA
6755
PRIMER_RIGHT_3_SEQUENCE=GGAACTCACCAACCCTTGTT
6756
PRIMER_LEFT_3=12,20
6757
PRIMER_RIGHT_3=173,20
6758
PRIMER_LEFT_3_TM=58.847
6759
PRIMER_RIGHT_3_TM=58.891
6760
PRIMER_LEFT_3_GC_PERCENT=45.000
6761
PRIMER_RIGHT_3_GC_PERCENT=50.000
6762
PRIMER_LEFT_3_SELF_ANY=4.00
6763
PRIMER_RIGHT_3_SELF_ANY=3.00
6764
PRIMER_LEFT_3_SELF_END=0.00
6765
PRIMER_RIGHT_3_SELF_END=3.00
6766
PRIMER_LEFT_3_END_STABILITY=7.0000
6767
PRIMER_RIGHT_3_END_STABILITY=7.0000
6768
PRIMER_PAIR_3_COMPL_ANY=3.00
6769
PRIMER_PAIR_3_COMPL_END=2.00
6770
PRIMER_PRODUCT_SIZE_3=162
6771
PRIMER_PAIR_PENALTY_4=2.2663
6772
PRIMER_LEFT_4_PENALTY=1.152564
6773
PRIMER_RIGHT_4_PENALTY=1.113753
6774
PRIMER_LEFT_4_SEQUENCE=CCTCCACTGCAATCAAAAGA
6775
PRIMER_RIGHT_4_SEQUENCE=AGGAACTCACCAACCCTTGTT
6776
PRIMER_LEFT_4=12,20
6777
PRIMER_RIGHT_4=174,21
6778
PRIMER_LEFT_4_TM=58.847
6779
PRIMER_RIGHT_4_TM=59.886
6780
PRIMER_LEFT_4_GC_PERCENT=45.000
6781
PRIMER_RIGHT_4_GC_PERCENT=47.619
6782
PRIMER_LEFT_4_SELF_ANY=4.00
6783
PRIMER_RIGHT_4_SELF_ANY=3.00
6784
PRIMER_LEFT_4_SELF_END=0.00
6785
PRIMER_RIGHT_4_SELF_END=3.00
6786
PRIMER_LEFT_4_END_STABILITY=7.0000
6787
PRIMER_RIGHT_4_END_STABILITY=7.0000
6788
PRIMER_PAIR_4_COMPL_ANY=3.00
6789
PRIMER_PAIR_4_COMPL_END=2.00
6790
PRIMER_PRODUCT_SIZE_4=163
6791
=
6792
MARKER_NAME=06.RHAP64FB3.seq
6793
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCCTTTAAATGATCAAGGAGTATCAAAANTTCCTCATTTGTGAATANANCTACGTGTATGNGTGTGNNAANGNGNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGTATCCGTACATGGATCAGAGAAATATAATCCTAAAAATCACAAAAATGACATAAAATGACATCTGTTATTTAAAAAAATCACCTCAGTTCAATGAGAACTAAAATATTTAAATCAACTACAGGAAAAAAAAACACCCCAGCGATTACACACACTGATTCAAAGATTGTGGCACCATTGACATATATTTCATTTTTGNTATAAAGTTTAANGTTTTGTAGGGNTGACTTGAACCCTGAAGAAGGTACCCAGGGTGCAGGTCAGTGCTGCATATGTTATTTACCAGTGCTAGACACTCAAACTTCAGGGTNNNCAC
6794
INCLUDED_REGION=21,421
6795
TARGET=97,28,GT
6796
PRIMER_COMMENT=CL is CCTTTAAATGATCAAGGAGTATCAA
6797
PRIMER_COMMENT=CR is TTTCTCTGATCCATGTACGGA
6798
PRIMER_PAIR_PENALTY=9.9455
6799
PRIMER_LEFT_PENALTY=7.104630
6800
PRIMER_RIGHT_PENALTY=2.840827
6801
PRIMER_LEFT_SEQUENCE=CCTTTAAATGATCAAGGAGTATCAA
6802
PRIMER_RIGHT_SEQUENCE=TTTCTCTGATCCATGTACGGA
6803
PRIMER_LEFT=21,25
6804
PRIMER_RIGHT=150,21
6805
PRIMER_LEFT_TM=57.895
6806
PRIMER_RIGHT_TM=58.159
6807
PRIMER_LEFT_GC_PERCENT=32.000
6808
PRIMER_RIGHT_GC_PERCENT=42.857
6809
PRIMER_LEFT_SELF_ANY=6.00
6810
PRIMER_RIGHT_SELF_ANY=4.00
6811
PRIMER_LEFT_SELF_END=0.00
6812
PRIMER_RIGHT_SELF_END=4.00
6813
PRIMER_LEFT_END_STABILITY=6.9000
6814
PRIMER_RIGHT_END_STABILITY=9.6000
6815
PRIMER_PAIR_COMPL_ANY=6.00
6816
PRIMER_PAIR_COMPL_END=3.00
6817
PRIMER_PRODUCT_SIZE=130
6818
PRIMER_PAIR_PENALTY_1=10.5751
6819
PRIMER_LEFT_1_PENALTY=7.104630
6820
PRIMER_RIGHT_1_PENALTY=3.470456
6821
PRIMER_LEFT_1_SEQUENCE=CCTTTAAATGATCAAGGAGTATCAA
6822
PRIMER_RIGHT_1_SEQUENCE=ATTTCTCTGATCCATGTACGGA
6823
PRIMER_LEFT_1=21,25
6824
PRIMER_RIGHT_1=151,22
6825
PRIMER_LEFT_1_TM=57.895
6826
PRIMER_RIGHT_1_TM=58.530
6827
PRIMER_LEFT_1_GC_PERCENT=32.000
6828
PRIMER_RIGHT_1_GC_PERCENT=40.909
6829
PRIMER_LEFT_1_SELF_ANY=6.00
6830
PRIMER_RIGHT_1_SELF_ANY=4.00
6831
PRIMER_LEFT_1_SELF_END=0.00
6832
PRIMER_RIGHT_1_SELF_END=4.00
6833
PRIMER_LEFT_1_END_STABILITY=6.9000
6834
PRIMER_RIGHT_1_END_STABILITY=9.6000
6835
PRIMER_PAIR_1_COMPL_ANY=6.00
6836
PRIMER_PAIR_1_COMPL_END=4.00
6837
PRIMER_PRODUCT_SIZE_1=131
6838
PRIMER_PAIR_PENALTY_2=10.5751
6839
PRIMER_LEFT_2_PENALTY=7.104630
6840
PRIMER_RIGHT_2_PENALTY=3.470456
6841
PRIMER_LEFT_2_SEQUENCE=CCTTTAAATGATCAAGGAGTATCAA
6842
PRIMER_RIGHT_2_SEQUENCE=TTTCTCTGATCCATGTACGGAT
6843
PRIMER_LEFT_2=21,25
6844
PRIMER_RIGHT_2=150,22
6845
PRIMER_LEFT_2_TM=57.895
6846
PRIMER_RIGHT_2_TM=58.530
6847
PRIMER_LEFT_2_GC_PERCENT=32.000
6848
PRIMER_RIGHT_2_GC_PERCENT=40.909
6849
PRIMER_LEFT_2_SELF_ANY=6.00
6850
PRIMER_RIGHT_2_SELF_ANY=6.00
6851
PRIMER_LEFT_2_SELF_END=0.00
6852
PRIMER_RIGHT_2_SELF_END=6.00
6853
PRIMER_LEFT_2_END_STABILITY=6.9000
6854
PRIMER_RIGHT_2_END_STABILITY=9.8000
6855
PRIMER_PAIR_2_COMPL_ANY=6.00
6856
PRIMER_PAIR_2_COMPL_END=1.00
6857
PRIMER_PRODUCT_SIZE_2=130
6858
PRIMER_PAIR_PENALTY_3=11.0442
6859
PRIMER_LEFT_3_PENALTY=7.104630
6860
PRIMER_RIGHT_3_PENALTY=3.939538
6861
PRIMER_LEFT_3_SEQUENCE=CCTTTAAATGATCAAGGAGTATCAA
6862
PRIMER_RIGHT_3_SEQUENCE=TTCTCTGATCCATGTACGGAT
6863
PRIMER_LEFT_3=21,25
6864
PRIMER_RIGHT_3=149,21
6865
PRIMER_LEFT_3_TM=57.895
6866
PRIMER_RIGHT_3_TM=57.060
6867
PRIMER_LEFT_3_GC_PERCENT=32.000
6868
PRIMER_RIGHT_3_GC_PERCENT=42.857
6869
PRIMER_LEFT_3_SELF_ANY=6.00
6870
PRIMER_RIGHT_3_SELF_ANY=6.00
6871
PRIMER_LEFT_3_SELF_END=0.00
6872
PRIMER_RIGHT_3_SELF_END=6.00
6873
PRIMER_LEFT_3_END_STABILITY=6.9000
6874
PRIMER_RIGHT_3_END_STABILITY=9.8000
6875
PRIMER_PAIR_3_COMPL_ANY=6.00
6876
PRIMER_PAIR_3_COMPL_END=2.00
6877
PRIMER_PRODUCT_SIZE_3=129
6878
PRIMER_PAIR_PENALTY_4=11.2423
6879
PRIMER_LEFT_4_PENALTY=7.104630
6880
PRIMER_RIGHT_4_PENALTY=4.137685
6881
PRIMER_LEFT_4_SEQUENCE=CCTTTAAATGATCAAGGAGTATCAA
6882
PRIMER_RIGHT_4_SEQUENCE=ATTTCTCTGATCCATGTACGGAT
6883
PRIMER_LEFT_4=21,25
6884
PRIMER_RIGHT_4=151,23
6885
PRIMER_LEFT_4_TM=57.895
6886
PRIMER_RIGHT_4_TM=58.862
6887
PRIMER_LEFT_4_GC_PERCENT=32.000
6888
PRIMER_RIGHT_4_GC_PERCENT=39.130
6889
PRIMER_LEFT_4_SELF_ANY=6.00
6890
PRIMER_RIGHT_4_SELF_ANY=6.00
6891
PRIMER_LEFT_4_SELF_END=0.00
6892
PRIMER_RIGHT_4_SELF_END=6.00
6893
PRIMER_LEFT_4_END_STABILITY=6.9000
6894
PRIMER_RIGHT_4_END_STABILITY=9.8000
6895
PRIMER_PAIR_4_COMPL_ANY=6.00
6896
PRIMER_PAIR_4_COMPL_END=2.00
6897
PRIMER_PRODUCT_SIZE_4=131
6898
=
6899
MARKER_NAME=07.RHAP64FC7.seq
6900
SEQUENCE=NNNNNNNNNNNNCCAGCCAAANATCGTCAACAAACGCCTGTGACNACCCCCCAAGATCAAAATCCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGTGTCAGTGTCTCTCCCNGCACCCATGGACATATGCATGTGCCGACCCAATANGCGTCCATNTATGCATATCCATGTGCATCTGNNCATGTATGTGCACATGTGTGTCTCTCAGTGTGGGTGNACAGGNGTANGGGACATGTGTGCACATGGGCGCANTCCAAATAATCNGGAACTCTTCCCCAGCTTCACCTTGAAGGCATTCCCAGGCGCAAGCAANAGCTANCGTAACCACNGGCAGCATTCTCTNCTCNCCTCCGACCCCTNTAGCTCTCGCCNCATCAGGCCGTAGGCTGNCCCAGCTTCAAACTGNTAAGAGCNTGTCACCCTCCANNCAGAGGNACCCGTGNGNGTGNGGGTCNGC
6901
INCLUDED_REGION=12,448
6902
TARGET=67,29,TG
6903
PRIMER_COMMENT=CL is CCCCCAAGATCAAAATCCAC
6904
PRIMER_COMMENT=CR is ACCCACACTGAGAGACACACA
6905
PRIMER_PAIR_PENALTY=3.2602
6906
PRIMER_LEFT_PENALTY=1.441475
6907
PRIMER_RIGHT_PENALTY=1.818694
6908
PRIMER_LEFT_SEQUENCE=CCCCCAAGATCAAAATCCAC
6909
PRIMER_RIGHT_SEQUENCE=CACCCACACTGAGAGACACAC
6910
PRIMER_LEFT=47,20
6911
PRIMER_RIGHT=218,21
6912
PRIMER_LEFT_TM=61.441
6913
PRIMER_RIGHT_TM=59.181
6914
PRIMER_LEFT_GC_PERCENT=50.000
6915
PRIMER_RIGHT_GC_PERCENT=57.143
6916
PRIMER_LEFT_SELF_ANY=4.00
6917
PRIMER_RIGHT_SELF_ANY=3.00
6918
PRIMER_LEFT_SELF_END=0.00
6919
PRIMER_RIGHT_SELF_END=1.00
6920
PRIMER_LEFT_END_STABILITY=7.9000
6921
PRIMER_RIGHT_END_STABILITY=6.4000
6922
PRIMER_PAIR_COMPL_ANY=3.00
6923
PRIMER_PAIR_COMPL_END=0.00
6924
PRIMER_PRODUCT_SIZE=172
6925
PRIMER_PAIR_PENALTY_1=3.2602
6926
PRIMER_LEFT_1_PENALTY=1.441475
6927
PRIMER_RIGHT_1_PENALTY=1.818694
6928
PRIMER_LEFT_1_SEQUENCE=CCCCCAAGATCAAAATCCAC
6929
PRIMER_RIGHT_1_SEQUENCE=ACCCACACTGAGAGACACACA
6930
PRIMER_LEFT_1=47,20
6931
PRIMER_RIGHT_1=217,21
6932
PRIMER_LEFT_1_TM=61.441
6933
PRIMER_RIGHT_1_TM=59.181
6934
PRIMER_LEFT_1_GC_PERCENT=50.000
6935
PRIMER_RIGHT_1_GC_PERCENT=52.381
6936
PRIMER_LEFT_1_SELF_ANY=4.00
6937
PRIMER_RIGHT_1_SELF_ANY=3.00
6938
PRIMER_LEFT_1_SELF_END=0.00
6939
PRIMER_RIGHT_1_SELF_END=3.00
6940
PRIMER_LEFT_1_END_STABILITY=7.9000
6941
PRIMER_RIGHT_1_END_STABILITY=6.4000
6942
PRIMER_PAIR_1_COMPL_ANY=3.00
6943
PRIMER_PAIR_1_COMPL_END=0.00
6944
PRIMER_PRODUCT_SIZE_1=171
6945
PRIMER_PAIR_PENALTY_2=3.2876
6946
PRIMER_LEFT_2_PENALTY=1.441475
6947
PRIMER_RIGHT_2_PENALTY=1.846163
6948
PRIMER_LEFT_2_SEQUENCE=CCCCCAAGATCAAAATCCAC
6949
PRIMER_RIGHT_2_SEQUENCE=CACCCACACTGAGAGACACA
6950
PRIMER_LEFT_2=47,20
6951
PRIMER_RIGHT_2=218,20
6952
PRIMER_LEFT_2_TM=61.441
6953
PRIMER_RIGHT_2_TM=58.154
6954
PRIMER_LEFT_2_GC_PERCENT=50.000
6955
PRIMER_RIGHT_2_GC_PERCENT=55.000
6956
PRIMER_LEFT_2_SELF_ANY=4.00
6957
PRIMER_RIGHT_2_SELF_ANY=3.00
6958
PRIMER_LEFT_2_SELF_END=0.00
6959
PRIMER_RIGHT_2_SELF_END=1.00
6960
PRIMER_LEFT_2_END_STABILITY=7.9000
6961
PRIMER_RIGHT_2_END_STABILITY=6.4000
6962
PRIMER_PAIR_2_COMPL_ANY=3.00
6963
PRIMER_PAIR_2_COMPL_END=0.00
6964
PRIMER_PRODUCT_SIZE_2=172
6965
PRIMER_PAIR_PENALTY_3=3.2876
6966
PRIMER_LEFT_3_PENALTY=1.441475
6967
PRIMER_RIGHT_3_PENALTY=1.846163
6968
PRIMER_LEFT_3_SEQUENCE=CCCCCAAGATCAAAATCCAC
6969
PRIMER_RIGHT_3_SEQUENCE=CCCACACTGAGAGACACACA
6970
PRIMER_LEFT_3=47,20
6971
PRIMER_RIGHT_3=216,20
6972
PRIMER_LEFT_3_TM=61.441
6973
PRIMER_RIGHT_3_TM=58.154
6974
PRIMER_LEFT_3_GC_PERCENT=50.000
6975
PRIMER_RIGHT_3_GC_PERCENT=55.000
6976
PRIMER_LEFT_3_SELF_ANY=4.00
6977
PRIMER_RIGHT_3_SELF_ANY=3.00
6978
PRIMER_LEFT_3_SELF_END=0.00
6979
PRIMER_RIGHT_3_SELF_END=3.00
6980
PRIMER_LEFT_3_END_STABILITY=7.9000
6981
PRIMER_RIGHT_3_END_STABILITY=6.4000
6982
PRIMER_PAIR_3_COMPL_ANY=3.00
6983
PRIMER_PAIR_3_COMPL_END=0.00
6984
PRIMER_PRODUCT_SIZE_3=170
6985
PRIMER_PAIR_PENALTY_4=3.4627
6986
PRIMER_LEFT_4_PENALTY=1.643998
6987
PRIMER_RIGHT_4_PENALTY=1.818694
6988
PRIMER_LEFT_4_SEQUENCE=CCCCCAAGATCAAAATCCA
6989
PRIMER_RIGHT_4_SEQUENCE=CACCCACACTGAGAGACACAC
6990
PRIMER_LEFT_4=47,19
6991
PRIMER_RIGHT_4=218,21
6992
PRIMER_LEFT_4_TM=60.644
6993
PRIMER_RIGHT_4_TM=59.181
6994
PRIMER_LEFT_4_GC_PERCENT=47.368
6995
PRIMER_RIGHT_4_GC_PERCENT=57.143
6996
PRIMER_LEFT_4_SELF_ANY=4.00
6997
PRIMER_RIGHT_4_SELF_ANY=3.00
6998
PRIMER_LEFT_4_SELF_END=0.00
6999
PRIMER_RIGHT_4_SELF_END=1.00
7000
PRIMER_LEFT_4_END_STABILITY=8.1000
7001
PRIMER_RIGHT_4_END_STABILITY=6.4000
7002
PRIMER_PAIR_4_COMPL_ANY=3.00
7003
PRIMER_PAIR_4_COMPL_END=0.00
7004
PRIMER_PRODUCT_SIZE_4=172
7005
=
7006
MARKER_NAME=09.RHAP64FC3.seq
7007
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCTGACATTCTCTTCCGACCTCTGCAGCCATCAGCATATGTGGTGCACAGACATATGTGTAGGCAAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATACAATTGTTTTTAAGGAAAAAAGTAAAAACAAACAGGAAGAAGAAATGTCTGGTCTACCTTAGGGCCCAGGTTTTAGTGTAACAAAGGCTTGTCTCCTCCCAGGACTTACCATGTTCCCCTTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
7008
INCLUDED_REGION=21,239
7009
TARGET=89,45,AC
7010
PRIMER_COMMENT=CL is CTGCAGCCATCAGCATATGT
7011
PRIMER_COMMENT=CR is AACCTGGGCCCTAAGGTAGA
7012
PRIMER_PAIR_PENALTY=0.1911
7013
PRIMER_LEFT_PENALTY=0.146193
7014
PRIMER_RIGHT_PENALTY=0.044904
7015
PRIMER_LEFT_SEQUENCE=CTGCAGCCATCAGCATATGT
7016
PRIMER_RIGHT_SEQUENCE=AACCTGGGCCCTAAGGTAGA
7017
PRIMER_LEFT=41,20
7018
PRIMER_RIGHT=208,20
7019
PRIMER_LEFT_TM=59.854
7020
PRIMER_RIGHT_TM=59.955
7021
PRIMER_LEFT_GC_PERCENT=50.000
7022
PRIMER_RIGHT_GC_PERCENT=55.000
7023
PRIMER_LEFT_SELF_ANY=6.00
7024
PRIMER_RIGHT_SELF_ANY=6.00
7025
PRIMER_LEFT_SELF_END=4.00
7026
PRIMER_RIGHT_SELF_END=1.00
7027
PRIMER_LEFT_END_STABILITY=5.6000
7028
PRIMER_RIGHT_END_STABILITY=5.4000
7029
PRIMER_PAIR_COMPL_ANY=3.00
7030
PRIMER_PAIR_COMPL_END=1.00
7031
PRIMER_PRODUCT_SIZE=168
7032
PRIMER_PAIR_PENALTY_1=0.1929
7033
PRIMER_LEFT_1_PENALTY=0.146193
7034
PRIMER_RIGHT_1_PENALTY=0.046746
7035
PRIMER_LEFT_1_SEQUENCE=CTGCAGCCATCAGCATATGT
7036
PRIMER_RIGHT_1_SEQUENCE=CTAAAACCTGGGCCCTAAGG
7037
PRIMER_LEFT_1=41,20
7038
PRIMER_RIGHT_1=212,20
7039
PRIMER_LEFT_1_TM=59.854
7040
PRIMER_RIGHT_1_TM=59.953
7041
PRIMER_LEFT_1_GC_PERCENT=50.000
7042
PRIMER_RIGHT_1_GC_PERCENT=55.000
7043
PRIMER_LEFT_1_SELF_ANY=6.00
7044
PRIMER_RIGHT_1_SELF_ANY=6.00
7045
PRIMER_LEFT_1_SELF_END=4.00
7046
PRIMER_RIGHT_1_SELF_END=5.00
7047
PRIMER_LEFT_1_END_STABILITY=5.6000
7048
PRIMER_RIGHT_1_END_STABILITY=7.5000
7049
PRIMER_PAIR_1_COMPL_ANY=3.00
7050
PRIMER_PAIR_1_COMPL_END=0.00
7051
PRIMER_PRODUCT_SIZE_1=172
7052
PRIMER_PAIR_PENALTY_2=0.3167
7053
PRIMER_LEFT_2_PENALTY=0.146193
7054
PRIMER_RIGHT_2_PENALTY=0.170538
7055
PRIMER_LEFT_2_SEQUENCE=CTGCAGCCATCAGCATATGT
7056
PRIMER_RIGHT_2_SEQUENCE=AAAACCTGGGCCCTAAGGTA
7057
PRIMER_LEFT_2=41,20
7058
PRIMER_RIGHT_2=210,20
7059
PRIMER_LEFT_2_TM=59.854
7060
PRIMER_RIGHT_2_TM=59.829
7061
PRIMER_LEFT_2_GC_PERCENT=50.000
7062
PRIMER_RIGHT_2_GC_PERCENT=50.000
7063
PRIMER_LEFT_2_SELF_ANY=6.00
7064
PRIMER_RIGHT_2_SELF_ANY=6.00
7065
PRIMER_LEFT_2_SELF_END=4.00
7066
PRIMER_RIGHT_2_SELF_END=4.00
7067
PRIMER_LEFT_2_END_STABILITY=5.6000
7068
PRIMER_RIGHT_2_END_STABILITY=6.9000
7069
PRIMER_PAIR_2_COMPL_ANY=3.00
7070
PRIMER_PAIR_2_COMPL_END=1.00
7071
PRIMER_PRODUCT_SIZE_2=170
7072
PRIMER_PAIR_PENALTY_3=0.3167
7073
PRIMER_LEFT_3_PENALTY=0.146193
7074
PRIMER_RIGHT_3_PENALTY=0.170538
7075
PRIMER_LEFT_3_SEQUENCE=CTGCAGCCATCAGCATATGT
7076
PRIMER_RIGHT_3_SEQUENCE=TAAAACCTGGGCCCTAAGGT
7077
PRIMER_LEFT_3=41,20
7078
PRIMER_RIGHT_3=211,20
7079
PRIMER_LEFT_3_TM=59.854
7080
PRIMER_RIGHT_3_TM=59.829
7081
PRIMER_LEFT_3_GC_PERCENT=50.000
7082
PRIMER_RIGHT_3_GC_PERCENT=50.000
7083
PRIMER_LEFT_3_SELF_ANY=6.00
7084
PRIMER_RIGHT_3_SELF_ANY=6.00
7085
PRIMER_LEFT_3_SELF_END=4.00
7086
PRIMER_RIGHT_3_SELF_END=6.00
7087
PRIMER_LEFT_3_END_STABILITY=5.6000
7088
PRIMER_RIGHT_3_END_STABILITY=7.9000
7089
PRIMER_PAIR_3_COMPL_ANY=3.00
7090
PRIMER_PAIR_3_COMPL_END=1.00
7091
PRIMER_PRODUCT_SIZE_3=171
7092
PRIMER_PAIR_PENALTY_4=0.4927
7093
PRIMER_LEFT_4_PENALTY=0.447804
7094
PRIMER_RIGHT_4_PENALTY=0.044904
7095
PRIMER_LEFT_4_SEQUENCE=CAGCCATCAGCATATGTGGT
7096
PRIMER_RIGHT_4_SEQUENCE=AACCTGGGCCCTAAGGTAGA
7097
PRIMER_LEFT_4=44,20
7098
PRIMER_RIGHT_4=208,20
7099
PRIMER_LEFT_4_TM=59.552
7100
PRIMER_RIGHT_4_TM=59.955
7101
PRIMER_LEFT_4_GC_PERCENT=50.000
7102
PRIMER_RIGHT_4_GC_PERCENT=55.000
7103
PRIMER_LEFT_4_SELF_ANY=6.00
7104
PRIMER_RIGHT_4_SELF_ANY=6.00
7105
PRIMER_LEFT_4_SELF_END=6.00
7106
PRIMER_RIGHT_4_SELF_END=1.00
7107
PRIMER_LEFT_4_END_STABILITY=7.6000
7108
PRIMER_RIGHT_4_END_STABILITY=5.4000
7109
PRIMER_PAIR_4_COMPL_ANY=3.00
7110
PRIMER_PAIR_4_COMPL_END=1.00
7111
PRIMER_PRODUCT_SIZE_4=165
7112
=
7113
MARKER_NAME=11.RHAP64FD8.seq
7114
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCTTAATTAATGCTTACTGACGGAAAAAAGGACAGGGACAATAATCACCTACTGAACAAAGAAAGTGATATGTATGGAAAANTAAAAGCAGATTATTTCACATAATGTAAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATACATGCTTGCAGCGGGTGCCTATCTTAATCCCAGCAGGCAGACTGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
7115
INCLUDED_REGION=23,193
7116
TARGET=135,30,TG
7117
PRIMER_COMMENT=CL is CTGACGGAAAAAAGGACAGG
7118
PRIMER_COMMENT=CR is GTCTGCCTGCTGGGATTAAG
7119
PRIMER_PAIR_PENALTY=0.4584
7120
PRIMER_LEFT_PENALTY=0.294694
7121
PRIMER_RIGHT_PENALTY=0.163658
7122
PRIMER_LEFT_SEQUENCE=CTGACGGAAAAAAGGACAGG
7123
PRIMER_RIGHT_SEQUENCE=GTCTGCCTGCTGGGATTAAG
7124
PRIMER_LEFT=38,20
7125
PRIMER_RIGHT=210,20
7126
PRIMER_LEFT_TM=59.705
7127
PRIMER_RIGHT_TM=59.836
7128
PRIMER_LEFT_GC_PERCENT=50.000
7129
PRIMER_RIGHT_GC_PERCENT=55.000
7130
PRIMER_LEFT_SELF_ANY=3.00
7131
PRIMER_RIGHT_SELF_ANY=4.00
7132
PRIMER_LEFT_SELF_END=0.00
7133
PRIMER_RIGHT_SELF_END=2.00
7134
PRIMER_LEFT_END_STABILITY=7.9000
7135
PRIMER_RIGHT_END_STABILITY=6.3000
7136
PRIMER_PAIR_COMPL_ANY=4.00
7137
PRIMER_PAIR_COMPL_END=0.00
7138
PRIMER_PRODUCT_SIZE=173
7139
PRIMER_PAIR_PENALTY_1=0.4584
7140
PRIMER_LEFT_1_PENALTY=0.294694
7141
PRIMER_RIGHT_1_PENALTY=0.163658
7142
PRIMER_LEFT_1_SEQUENCE=CTGACGGAAAAAAGGACAGG
7143
PRIMER_RIGHT_1_SEQUENCE=AGTCTGCCTGCTGGGATTAA
7144
PRIMER_LEFT_1=38,20
7145
PRIMER_RIGHT_1=211,20
7146
PRIMER_LEFT_1_TM=59.705
7147
PRIMER_RIGHT_1_TM=59.836
7148
PRIMER_LEFT_1_GC_PERCENT=50.000
7149
PRIMER_RIGHT_1_GC_PERCENT=50.000
7150
PRIMER_LEFT_1_SELF_ANY=3.00
7151
PRIMER_RIGHT_1_SELF_ANY=4.00
7152
PRIMER_LEFT_1_SELF_END=0.00
7153
PRIMER_RIGHT_1_SELF_END=4.00
7154
PRIMER_LEFT_1_END_STABILITY=7.9000
7155
PRIMER_RIGHT_1_END_STABILITY=6.2000
7156
PRIMER_PAIR_1_COMPL_ANY=4.00
7157
PRIMER_PAIR_1_COMPL_END=0.00
7158
PRIMER_PRODUCT_SIZE_1=174
7159
PRIMER_PAIR_PENALTY_2=0.5041
7160
PRIMER_LEFT_2_PENALTY=0.340458
7161
PRIMER_RIGHT_2_PENALTY=0.163658
7162
PRIMER_LEFT_2_SEQUENCE=ACGGAAAAAAGGACAGGGAC
7163
PRIMER_RIGHT_2_SEQUENCE=GTCTGCCTGCTGGGATTAAG
7164
PRIMER_LEFT_2=41,20
7165
PRIMER_RIGHT_2=210,20
7166
PRIMER_LEFT_2_TM=60.340
7167
PRIMER_RIGHT_2_TM=59.836
7168
PRIMER_LEFT_2_GC_PERCENT=50.000
7169
PRIMER_RIGHT_2_GC_PERCENT=55.000
7170
PRIMER_LEFT_2_SELF_ANY=2.00
7171
PRIMER_RIGHT_2_SELF_ANY=4.00
7172
PRIMER_LEFT_2_SELF_END=1.00
7173
PRIMER_RIGHT_2_SELF_END=2.00
7174
PRIMER_LEFT_2_END_STABILITY=9.1000
7175
PRIMER_RIGHT_2_END_STABILITY=6.3000
7176
PRIMER_PAIR_2_COMPL_ANY=4.00
7177
PRIMER_PAIR_2_COMPL_END=1.00
7178
PRIMER_PRODUCT_SIZE_2=170
7179
PRIMER_PAIR_PENALTY_3=0.5041
7180
PRIMER_LEFT_3_PENALTY=0.340458
7181
PRIMER_RIGHT_3_PENALTY=0.163658
7182
PRIMER_LEFT_3_SEQUENCE=ACGGAAAAAAGGACAGGGAC
7183
PRIMER_RIGHT_3_SEQUENCE=AGTCTGCCTGCTGGGATTAA
7184
PRIMER_LEFT_3=41,20
7185
PRIMER_RIGHT_3=211,20
7186
PRIMER_LEFT_3_TM=60.340
7187
PRIMER_RIGHT_3_TM=59.836
7188
PRIMER_LEFT_3_GC_PERCENT=50.000
7189
PRIMER_RIGHT_3_GC_PERCENT=50.000
7190
PRIMER_LEFT_3_SELF_ANY=2.00
7191
PRIMER_RIGHT_3_SELF_ANY=4.00
7192
PRIMER_LEFT_3_SELF_END=1.00
7193
PRIMER_RIGHT_3_SELF_END=4.00
7194
PRIMER_LEFT_3_END_STABILITY=9.1000
7195
PRIMER_RIGHT_3_END_STABILITY=6.2000
7196
PRIMER_PAIR_3_COMPL_ANY=4.00
7197
PRIMER_PAIR_3_COMPL_END=0.00
7198
PRIMER_PRODUCT_SIZE_3=171
7199
PRIMER_PAIR_PENALTY_4=0.9478
7200
PRIMER_LEFT_4_PENALTY=0.294694
7201
PRIMER_RIGHT_4_PENALTY=0.653090
7202
PRIMER_LEFT_4_SEQUENCE=CTGACGGAAAAAAGGACAGG
7203
PRIMER_RIGHT_4_SEQUENCE=GGGATTAAGATAGGCACCCG
7204
PRIMER_LEFT_4=38,20
7205
PRIMER_RIGHT_4=199,20
7206
PRIMER_LEFT_4_TM=59.705
7207
PRIMER_RIGHT_4_TM=60.653
7208
PRIMER_LEFT_4_GC_PERCENT=50.000
7209
PRIMER_RIGHT_4_GC_PERCENT=55.000
7210
PRIMER_LEFT_4_SELF_ANY=3.00
7211
PRIMER_RIGHT_4_SELF_ANY=4.00
7212
PRIMER_LEFT_4_SELF_END=0.00
7213
PRIMER_RIGHT_4_SELF_END=2.00
7214
PRIMER_LEFT_4_END_STABILITY=7.9000
7215
PRIMER_RIGHT_4_END_STABILITY=11.1000
7216
PRIMER_PAIR_4_COMPL_ANY=3.00
7217
PRIMER_PAIR_4_COMPL_END=2.00
7218
PRIMER_PRODUCT_SIZE_4=162
7219
=
7220
MARKER_NAME=12.RHAP64FD9.seq
7221
SEQUENCE=NNNNNNNNNNNNNCCACTATATCCCCTGCCTTTGTGGACCTGAGCATCTCATTGACGAATCCCAAATTATACCCCCTTCATTATTCATTAAAGTGTGGTGGGCAGAAATGTGTGTTCACGAATTCAGCTATGGAGAGGTTATAACTGAAGCCTCTGAATGCCCCACAGGGACCAATGTCAGCCAGTTCTATGCCCCTGTTCAGTATCTGCTTGAATCTGAAGCCGGACTCTGTATGTATGTGTATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATAAGANANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCANATATATATANNTTNTCNNNGGGGGGGGGGGGGNGAGGGAGAANANNNNNNCGGAGGNNNTANNNTGNGCNAGGTGNNNNNNTATNNGNNNACNCNCNTCNNGGGGC
7222
INCLUDED_REGION=13,445
7223
TARGET=246,44,TA
7224
TARGET=302,46,GT
7225
PRIMER_COMMENT=CL is
7226
PRIMER_COMMENT=CR is
7227
=
7228
MARKER_NAME=14.RHAP64FE2.seq
7229
SEQUENCE=NNNNNNNNNNNNNNNNNNNCCTCCACATGTGCACAGTGGTATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTACCTCATACCAANNNNNNNNNNNNNNNNNNNNNNNNNNGCATNNNNNNNNNNNNNNTATATATCTCAGGCACTTACACTATATNNNNNNNNNNNNNNNNNNNNATCACATNNNNNNNNNNNNNNNNNNNNNNNNNNATATGACAGGCAAAAATGAATTTATNNTCTCACATACATACATCACAGGCNTTCACACCATACACACATATTNNNNNNNNNNNNNNTGTATATNTCTTAGGCACTTACACTGTATATACACCACACACAAACTANNTAGGGNTTCACACCTTNNNCTCAACNCCCNCANAGGAGCANATGTGTGTTT
7230
INCLUDED_REGION=19,432
7231
TARGET=44,46,AC
7232
TARGET=92,33,CA
7233
TARGET=140,26,AC
7234
TARGET=170,14,CA
7235
TARGET=211,20,AC
7236
TARGET=238,26,AC
7237
TARGET=336,14,CA
7238
PRIMER_COMMENT=CL is CACATGTGCACAGTGGTATGT
7239
PRIMER_COMMENT=CR is GCCTGTGATGTATGTATGTGAGA
7240
PRIMER_PAIR_PENALTY=6.4586
7241
PRIMER_LEFT_PENALTY=1.104149
7242
PRIMER_RIGHT_PENALTY=5.354412
7243
PRIMER_LEFT_SEQUENCE=TCCACATGTGCACAGTGGTAT
7244
PRIMER_RIGHT_SEQUENCE=GCCTGTGATGTATGTATGTGAGA
7245
PRIMER_LEFT=21,21
7246
PRIMER_RIGHT=313,23
7247
PRIMER_LEFT_TM=59.896
7248
PRIMER_RIGHT_TM=57.646
7249
PRIMER_LEFT_GC_PERCENT=47.619
7250
PRIMER_RIGHT_GC_PERCENT=43.478
7251
PRIMER_LEFT_SELF_ANY=12.00
7252
PRIMER_RIGHT_SELF_ANY=2.00
7253
PRIMER_LEFT_SELF_END=6.00
7254
PRIMER_RIGHT_SELF_END=1.00
7255
PRIMER_LEFT_END_STABILITY=6.8000
7256
PRIMER_RIGHT_END_STABILITY=6.7000
7257
PRIMER_PAIR_COMPL_ANY=5.00
7258
PRIMER_PAIR_COMPL_END=1.00
7259
PRIMER_PRODUCT_SIZE=293
7260
PRIMER_PAIR_PENALTY_1=8.4675
7261
PRIMER_LEFT_1_PENALTY=3.113106
7262
PRIMER_RIGHT_1_PENALTY=5.354412
7263
PRIMER_LEFT_1_SEQUENCE=CACATGTGCACAGTGGTATGT
7264
PRIMER_RIGHT_1_SEQUENCE=GCCTGTGATGTATGTATGTGAGA
7265
PRIMER_LEFT_1=23,21
7266
PRIMER_RIGHT_1=313,23
7267
PRIMER_LEFT_1_TM=57.887
7268
PRIMER_RIGHT_1_TM=57.646
7269
PRIMER_LEFT_1_GC_PERCENT=47.619
7270
PRIMER_RIGHT_1_GC_PERCENT=43.478
7271
PRIMER_LEFT_1_SELF_ANY=10.00
7272
PRIMER_RIGHT_1_SELF_ANY=2.00
7273
PRIMER_LEFT_1_SELF_END=5.00
7274
PRIMER_RIGHT_1_SELF_END=1.00
7275
PRIMER_LEFT_1_END_STABILITY=5.6000
7276
PRIMER_RIGHT_1_END_STABILITY=6.7000
7277
PRIMER_PAIR_1_COMPL_ANY=5.00
7278
PRIMER_PAIR_1_COMPL_END=1.00
7279
PRIMER_PRODUCT_SIZE_1=291
7280
PRIMER_PAIR_PENALTY_2=8.7899
7281
PRIMER_LEFT_2_PENALTY=1.104149
7282
PRIMER_RIGHT_2_PENALTY=7.685778
7283
PRIMER_LEFT_2_SEQUENCE=TCCACATGTGCACAGTGGTAT
7284
PRIMER_RIGHT_2_SEQUENCE=ATAAATTCATTTTTGCCTGTCATAT
7285
PRIMER_LEFT_2=21,21
7286
PRIMER_RIGHT_2=288,25
7287
PRIMER_LEFT_2_TM=59.896
7288
PRIMER_RIGHT_2_TM=57.314
7289
PRIMER_LEFT_2_GC_PERCENT=47.619
7290
PRIMER_RIGHT_2_GC_PERCENT=24.000
7291
PRIMER_LEFT_2_SELF_ANY=12.00
7292
PRIMER_RIGHT_2_SELF_ANY=6.00
7293
PRIMER_LEFT_2_SELF_END=6.00
7294
PRIMER_RIGHT_2_SELF_END=4.00
7295
PRIMER_LEFT_2_END_STABILITY=6.8000
7296
PRIMER_RIGHT_2_END_STABILITY=5.8000
7297
PRIMER_PAIR_2_COMPL_ANY=5.00
7298
PRIMER_PAIR_2_COMPL_END=2.00
7299
PRIMER_PRODUCT_SIZE_2=268
7300
PRIMER_PAIR_PENALTY_3=9.1042
7301
PRIMER_LEFT_3_PENALTY=3.749784
7302
PRIMER_RIGHT_3_PENALTY=5.354412
7303
PRIMER_LEFT_3_SEQUENCE=CCACATGTGCACAGTGGTATGT
7304
PRIMER_RIGHT_3_SEQUENCE=GCCTGTGATGTATGTATGTGAGA
7305
PRIMER_LEFT_3=22,22
7306
PRIMER_RIGHT_3=313,23
7307
PRIMER_LEFT_3_TM=61.750
7308
PRIMER_RIGHT_3_TM=57.646
7309
PRIMER_LEFT_3_GC_PERCENT=50.000
7310
PRIMER_RIGHT_3_GC_PERCENT=43.478
7311
PRIMER_LEFT_3_SELF_ANY=12.00
7312
PRIMER_RIGHT_3_SELF_ANY=2.00
7313
PRIMER_LEFT_3_SELF_END=5.00
7314
PRIMER_RIGHT_3_SELF_END=1.00
7315
PRIMER_LEFT_3_END_STABILITY=5.6000
7316
PRIMER_RIGHT_3_END_STABILITY=6.7000
7317
PRIMER_PAIR_3_COMPL_ANY=5.00
7318
PRIMER_PAIR_3_COMPL_END=1.00
7319
PRIMER_PRODUCT_SIZE_3=292
7320
PRIMER_PAIR_PENALTY_4=10.1984
7321
PRIMER_LEFT_4_PENALTY=4.843946
7322
PRIMER_RIGHT_4_PENALTY=5.354412
7323
PRIMER_LEFT_4_SEQUENCE=TCCACATGTGCACAGTGGTATG
7324
PRIMER_RIGHT_4_SEQUENCE=GCCTGTGATGTATGTATGTGAGA
7325
PRIMER_LEFT_4=21,22
7326
PRIMER_RIGHT_4=313,23
7327
PRIMER_LEFT_4_TM=62.844
7328
PRIMER_RIGHT_4_TM=57.646
7329
PRIMER_LEFT_4_GC_PERCENT=50.000
7330
PRIMER_RIGHT_4_GC_PERCENT=43.478
7331
PRIMER_LEFT_4_SELF_ANY=12.00
7332
PRIMER_RIGHT_4_SELF_ANY=2.00
7333
PRIMER_LEFT_4_SELF_END=6.00
7334
PRIMER_RIGHT_4_SELF_END=1.00
7335
PRIMER_LEFT_4_END_STABILITY=5.6000
7336
PRIMER_RIGHT_4_END_STABILITY=6.7000
7337
PRIMER_PAIR_4_COMPL_ANY=5.00
7338
PRIMER_PAIR_4_COMPL_END=1.00
7339
PRIMER_PRODUCT_SIZE_4=293
7340
=
7341
MARKER_NAME=15.RHAP64FE3.seq
7342
SEQUENCE=NNNNNNNNNNNNNNNNNNNNCGACGACCTGACTTGGATCTTCTGGAACCACCTGGTAGAAGGGAGAATATGGTTGTCTTCAGACCACTGCAAGCCGTAGTAAATGGATGTAAATATATGTGCACACACAGAGTAATTATAAGTAATGAAAGAAAAGAAGTAAACACTTTCCATAATGTAAAGAAGAAGGCAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTGTATTCTTCACACAGTGACCCTCTCCACAGAGTCTCTCCTGTGTATTCCGCACACAGTGATCCTCTCCACCACCACCAATGACTTTTCTATAGCACGAATCTAATTCCGCTGGTAAAAGTCCTGGGTTGGCATGCCACAATTTTTCTTGTTCTTTTTAAAAGTAATTTTAGAGTAAATTTACAAAGTAATTNGTATTT
7343
INCLUDED_REGION=20,420
7344
TARGET=194,45,ATG
7345
PRIMER_COMMENT=CL is TCCATAATGTAAAGAAGAAGGCAA
7346
PRIMER_COMMENT=CR is TGGAGAGGATCACTGTGTGC
7347
PRIMER_PAIR_PENALTY=4.9608
7348
PRIMER_LEFT_PENALTY=4.787991
7349
PRIMER_RIGHT_PENALTY=0.172769
7350
PRIMER_LEFT_SEQUENCE=TCCATAATGTAAAGAAGAAGGCAA
7351
PRIMER_RIGHT_SEQUENCE=TGGAGAGGATCACTGTGTGC
7352
PRIMER_LEFT=168,24
7353
PRIMER_RIGHT=310,20
7354
PRIMER_LEFT_TM=59.212
7355
PRIMER_RIGHT_TM=59.827
7356
PRIMER_LEFT_GC_PERCENT=33.333
7357
PRIMER_RIGHT_GC_PERCENT=55.000
7358
PRIMER_LEFT_SELF_ANY=5.00
7359
PRIMER_RIGHT_SELF_ANY=5.00
7360
PRIMER_LEFT_SELF_END=0.00
7361
PRIMER_RIGHT_SELF_END=3.00
7362
PRIMER_LEFT_END_STABILITY=10.0000
7363
PRIMER_RIGHT_END_STABILITY=8.2000
7364
PRIMER_PAIR_COMPL_ANY=4.00
7365
PRIMER_PAIR_COMPL_END=2.00
7366
PRIMER_PRODUCT_SIZE=143
7367
PRIMER_PAIR_PENALTY_1=4.9608
7368
PRIMER_LEFT_1_PENALTY=4.787991
7369
PRIMER_RIGHT_1_PENALTY=0.172769
7370
PRIMER_LEFT_1_SEQUENCE=TTCCATAATGTAAAGAAGAAGGCA
7371
PRIMER_RIGHT_1_SEQUENCE=TGGAGAGGATCACTGTGTGC
7372
PRIMER_LEFT_1=167,24
7373
PRIMER_RIGHT_1=310,20
7374
PRIMER_LEFT_1_TM=59.212
7375
PRIMER_RIGHT_1_TM=59.827
7376
PRIMER_LEFT_1_GC_PERCENT=33.333
7377
PRIMER_RIGHT_1_GC_PERCENT=55.000
7378
PRIMER_LEFT_1_SELF_ANY=7.00
7379
PRIMER_RIGHT_1_SELF_ANY=5.00
7380
PRIMER_LEFT_1_SELF_END=0.00
7381
PRIMER_RIGHT_1_SELF_END=3.00
7382
PRIMER_LEFT_1_END_STABILITY=9.7000
7383
PRIMER_RIGHT_1_END_STABILITY=8.2000
7384
PRIMER_PAIR_1_COMPL_ANY=4.00
7385
PRIMER_PAIR_1_COMPL_END=3.00
7386
PRIMER_PRODUCT_SIZE_1=144
7387
PRIMER_PAIR_PENALTY_2=4.9631
7388
PRIMER_LEFT_2_PENALTY=4.787991
7389
PRIMER_RIGHT_2_PENALTY=0.175084
7390
PRIMER_LEFT_2_SEQUENCE=TCCATAATGTAAAGAAGAAGGCAA
7391
PRIMER_RIGHT_2_SEQUENCE=CTGTGTGCGGAATACACAGG
7392
PRIMER_LEFT_2=168,24
7393
PRIMER_RIGHT_2=298,20
7394
PRIMER_LEFT_2_TM=59.212
7395
PRIMER_RIGHT_2_TM=60.175
7396
PRIMER_LEFT_2_GC_PERCENT=33.333
7397
PRIMER_RIGHT_2_GC_PERCENT=55.000
7398
PRIMER_LEFT_2_SELF_ANY=5.00
7399
PRIMER_RIGHT_2_SELF_ANY=6.00
7400
PRIMER_LEFT_2_SELF_END=0.00
7401
PRIMER_RIGHT_2_SELF_END=3.00
7402
PRIMER_LEFT_2_END_STABILITY=10.0000
7403
PRIMER_RIGHT_2_END_STABILITY=7.9000
7404
PRIMER_PAIR_2_COMPL_ANY=4.00
7405
PRIMER_PAIR_2_COMPL_END=0.00
7406
PRIMER_PRODUCT_SIZE_2=131
7407
PRIMER_PAIR_PENALTY_3=4.9631
7408
PRIMER_LEFT_3_PENALTY=4.787991
7409
PRIMER_RIGHT_3_PENALTY=0.175084
7410
PRIMER_LEFT_3_SEQUENCE=TTCCATAATGTAAAGAAGAAGGCA
7411
PRIMER_RIGHT_3_SEQUENCE=CTGTGTGCGGAATACACAGG
7412
PRIMER_LEFT_3=167,24
7413
PRIMER_RIGHT_3=298,20
7414
PRIMER_LEFT_3_TM=59.212
7415
PRIMER_RIGHT_3_TM=60.175
7416
PRIMER_LEFT_3_GC_PERCENT=33.333
7417
PRIMER_RIGHT_3_GC_PERCENT=55.000
7418
PRIMER_LEFT_3_SELF_ANY=7.00
7419
PRIMER_RIGHT_3_SELF_ANY=6.00
7420
PRIMER_LEFT_3_SELF_END=0.00
7421
PRIMER_RIGHT_3_SELF_END=3.00
7422
PRIMER_LEFT_3_END_STABILITY=9.7000
7423
PRIMER_RIGHT_3_END_STABILITY=7.9000
7424
PRIMER_PAIR_3_COMPL_ANY=4.00
7425
PRIMER_PAIR_3_COMPL_END=0.00
7426
PRIMER_PRODUCT_SIZE_3=132
7427
PRIMER_PAIR_PENALTY_4=5.0513
7428
PRIMER_LEFT_4_PENALTY=4.787991
7429
PRIMER_RIGHT_4_PENALTY=0.263346
7430
PRIMER_LEFT_4_SEQUENCE=TCCATAATGTAAAGAAGAAGGCAA
7431
PRIMER_RIGHT_4_SEQUENCE=TGTGCGGAATACACAGGAGA
7432
PRIMER_LEFT_4=168,24
7433
PRIMER_RIGHT_4=295,20
7434
PRIMER_LEFT_4_TM=59.212
7435
PRIMER_RIGHT_4_TM=60.263
7436
PRIMER_LEFT_4_GC_PERCENT=33.333
7437
PRIMER_RIGHT_4_GC_PERCENT=50.000
7438
PRIMER_LEFT_4_SELF_ANY=5.00
7439
PRIMER_RIGHT_4_SELF_ANY=4.00
7440
PRIMER_LEFT_4_SELF_END=0.00
7441
PRIMER_RIGHT_4_SELF_END=0.00
7442
PRIMER_LEFT_4_END_STABILITY=10.0000
7443
PRIMER_RIGHT_4_END_STABILITY=7.9000
7444
PRIMER_PAIR_4_COMPL_ANY=4.00
7445
PRIMER_PAIR_4_COMPL_END=0.00
7446
PRIMER_PRODUCT_SIZE_4=128
7447
=
7448
MARKER_NAME=15.RHAP64FE9.seq
7449
SEQUENCE=NNNNNNNNNNNNNCCTGAGCACCAGAAACCACAGAGTTGTGCTATACNNNNTGAGCAATAGCCCAAGCTGCGTGTTAGCTCTGCACAGCATGGTGCTAACTGTGTTGTTTTGTCAATCCCTCACCACTANCTGTGNGTGTGCANNNNNNNNNNNNNNNNNNNNNNNGTGNNCANGNGGGTGAANGTGTATGNGTGNGCNTGCGNNNNNNNNNNNNNNNNNNGCATGNGNNNNNNNNNNNNNNNNNNNTATGNGNATGNGGCGNGAGNGTGTGNATGTGTGGTNTGCATGTATGTGNNNGTNCAATGTGGTANNNTNGTGNGGCANNCNTGNNNGTGTGNTGNTGGNNGTGNTAANNGGTGNAGNGTGNGNGCGGGCGNGNGNTGGTNTNGTGTGNGGTTGNGGNNNNGNGTGTNGNGGNNCNCNNGGANNGCGNNNNNNNNNCCGNGCNGGNTCNNGGNANTANTNGNNNANNGNNNGNANAGNNGAC
7450
INCLUDED_REGION=13,475
7451
TARGET=143,23,TG
7452
TARGET=204,15,GT
7453
TARGET=229,18,TG
7454
PRIMER_COMMENT=CL is
7455
PRIMER_COMMENT=CR is
7456
=
7457
MARKER_NAME=16.RHAP64FF7.seq
7458
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCATTCTTTGCTATTTATTGTTCCTGGGTAGCATTTCCTAAATACATCCATGAAGTGCTTTTTCTGTAGAAGGTTAACTTGTGTAACATGTGGCAGGACCTAGTACTGAGTGAGTTTGCCTTCACTTCAGTCTCACAAGAACTTGAATAGGCTGGTGTCTAATAATGGAGCTGTTGAAATGTTGGGNTCCTGAACTTTTCAGATTTGTCAATGGACTAGGGTCATGTGAAACACACATTGAAGAGAACTTTCAGTTGGACTNTTNAAGGGTANCCTTTTNNTTTTTAGAGATCTTNATNATTATTTTTTGCCACGNGTACNNNNNNNNNNNNNNNNNACTANGTGCATGTAAGTACAGACGCCTGTGGAAGGTGGAGANTTGGATCCCCTGGAGCTGGAGTTAAAGGCGGTTATAAGTTAGGAACTGAACTGAGGTCCT
7459
INCLUDED_REGION=23,438
7460
TARGET=343,16,TG
7461
PRIMER_COMMENT=CL is TTGTCAATGGACTAGGGTCATG
7462
PRIMER_COMMENT=CR is ACCTTCCACAGGCGTCTGTA
7463
PRIMER_PAIR_PENALTY=2.8585
7464
PRIMER_LEFT_PENALTY=2.147295
7465
PRIMER_RIGHT_PENALTY=0.711184
7466
PRIMER_LEFT_SEQUENCE=CAATGGACTAGGGTCATGTGAA
7467
PRIMER_RIGHT_SEQUENCE=CCTTCCACAGGCGTCTGTAC
7468
PRIMER_LEFT=230,22
7469
PRIMER_RIGHT=393,20
7470
PRIMER_LEFT_TM=59.853
7471
PRIMER_RIGHT_TM=60.711
7472
PRIMER_LEFT_GC_PERCENT=45.455
7473
PRIMER_RIGHT_GC_PERCENT=60.000
7474
PRIMER_LEFT_SELF_ANY=4.00
7475
PRIMER_RIGHT_SELF_ANY=8.00
7476
PRIMER_LEFT_SELF_END=2.00
7477
PRIMER_RIGHT_SELF_END=4.00
7478
PRIMER_LEFT_END_STABILITY=6.7000
7479
PRIMER_RIGHT_END_STABILITY=5.4000
7480
PRIMER_PAIR_COMPL_ANY=4.00
7481
PRIMER_PAIR_COMPL_END=3.00
7482
PRIMER_PRODUCT_SIZE=164
7483
PRIMER_PAIR_PENALTY_1=2.8585
7484
PRIMER_LEFT_1_PENALTY=2.147295
7485
PRIMER_RIGHT_1_PENALTY=0.711184
7486
PRIMER_LEFT_1_SEQUENCE=CAATGGACTAGGGTCATGTGAA
7487
PRIMER_RIGHT_1_SEQUENCE=ACCTTCCACAGGCGTCTGTA
7488
PRIMER_LEFT_1=230,22
7489
PRIMER_RIGHT_1=394,20
7490
PRIMER_LEFT_1_TM=59.853
7491
PRIMER_RIGHT_1_TM=60.711
7492
PRIMER_LEFT_1_GC_PERCENT=45.455
7493
PRIMER_RIGHT_1_GC_PERCENT=55.000
7494
PRIMER_LEFT_1_SELF_ANY=4.00
7495
PRIMER_RIGHT_1_SELF_ANY=8.00
7496
PRIMER_LEFT_1_SELF_END=2.00
7497
PRIMER_RIGHT_1_SELF_END=6.00
7498
PRIMER_LEFT_1_END_STABILITY=6.7000
7499
PRIMER_RIGHT_1_END_STABILITY=5.7000
7500
PRIMER_PAIR_1_COMPL_ANY=4.00
7501
PRIMER_PAIR_1_COMPL_END=2.00
7502
PRIMER_PRODUCT_SIZE_1=165
7503
PRIMER_PAIR_PENALTY_2=2.8585
7504
PRIMER_LEFT_2_PENALTY=2.147295
7505
PRIMER_RIGHT_2_PENALTY=0.711184
7506
PRIMER_LEFT_2_SEQUENCE=TTGTCAATGGACTAGGGTCATG
7507
PRIMER_RIGHT_2_SEQUENCE=CCTTCCACAGGCGTCTGTAC
7508
PRIMER_LEFT_2=226,22
7509
PRIMER_RIGHT_2=393,20
7510
PRIMER_LEFT_2_TM=59.853
7511
PRIMER_RIGHT_2_TM=60.711
7512
PRIMER_LEFT_2_GC_PERCENT=45.455
7513
PRIMER_RIGHT_2_GC_PERCENT=60.000
7514
PRIMER_LEFT_2_SELF_ANY=6.00
7515
PRIMER_RIGHT_2_SELF_ANY=8.00
7516
PRIMER_LEFT_2_SELF_END=4.00
7517
PRIMER_RIGHT_2_SELF_END=4.00
7518
PRIMER_LEFT_2_END_STABILITY=6.9000
7519
PRIMER_RIGHT_2_END_STABILITY=5.4000
7520
PRIMER_PAIR_2_COMPL_ANY=4.00
7521
PRIMER_PAIR_2_COMPL_END=1.00
7522
PRIMER_PRODUCT_SIZE_2=168
7523
PRIMER_PAIR_PENALTY_3=2.8585
7524
PRIMER_LEFT_3_PENALTY=2.147295
7525
PRIMER_RIGHT_3_PENALTY=0.711184
7526
PRIMER_LEFT_3_SEQUENCE=TTGTCAATGGACTAGGGTCATG
7527
PRIMER_RIGHT_3_SEQUENCE=ACCTTCCACAGGCGTCTGTA
7528
PRIMER_LEFT_3=226,22
7529
PRIMER_RIGHT_3=394,20
7530
PRIMER_LEFT_3_TM=59.853
7531
PRIMER_RIGHT_3_TM=60.711
7532
PRIMER_LEFT_3_GC_PERCENT=45.455
7533
PRIMER_RIGHT_3_GC_PERCENT=55.000
7534
PRIMER_LEFT_3_SELF_ANY=6.00
7535
PRIMER_RIGHT_3_SELF_ANY=8.00
7536
PRIMER_LEFT_3_SELF_END=4.00
7537
PRIMER_RIGHT_3_SELF_END=6.00
7538
PRIMER_LEFT_3_END_STABILITY=6.9000
7539
PRIMER_RIGHT_3_END_STABILITY=5.7000
7540
PRIMER_PAIR_3_COMPL_ANY=4.00
7541
PRIMER_PAIR_3_COMPL_END=0.00
7542
PRIMER_PRODUCT_SIZE_3=169
7543
PRIMER_PAIR_PENALTY_4=3.0863
7544
PRIMER_LEFT_4_PENALTY=2.375090
7545
PRIMER_RIGHT_4_PENALTY=0.711184
7546
PRIMER_LEFT_4_SEQUENCE=TCAATGGACTAGGGTCATGTGA
7547
PRIMER_RIGHT_4_SEQUENCE=CCTTCCACAGGCGTCTGTAC
7548
PRIMER_LEFT_4=229,22
7549
PRIMER_RIGHT_4=393,20
7550
PRIMER_LEFT_4_TM=60.375
7551
PRIMER_RIGHT_4_TM=60.711
7552
PRIMER_LEFT_4_GC_PERCENT=45.455
7553
PRIMER_RIGHT_4_GC_PERCENT=60.000
7554
PRIMER_LEFT_4_SELF_ANY=4.00
7555
PRIMER_RIGHT_4_SELF_ANY=8.00
7556
PRIMER_LEFT_4_SELF_END=4.00
7557
PRIMER_RIGHT_4_SELF_END=4.00
7558
PRIMER_LEFT_4_END_STABILITY=6.7000
7559
PRIMER_RIGHT_4_END_STABILITY=5.4000
7560
PRIMER_PAIR_4_COMPL_ANY=4.00
7561
PRIMER_PAIR_4_COMPL_END=2.00
7562
PRIMER_PRODUCT_SIZE_4=165
7563
=
7564
MARKER_NAME=17.RHAP64FF8.seq
7565
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCCCCAGTCCAAGTTTGCAGTAGTTCCCTAGTTAGGGAATATTTTTCTGATGACATAGGTGTAGTCTCATCACTGTGTGCTTACTGCCTAGATTAGCACAGAGCAAAGAATGAATTGGTTTTCCAGGGAAAATTGCTAAATTAGTAAACACGAGAGTAAGGCAGGAAAGAGCAACAATTTTACTCACAGGTTAATGGTTCACGTTTAGTCAAATTCCAAACTATGGCTCGGTGGTTGGTATAATTATAAGGACATGTTCTATGATTCCATCTCTTTATTAAATATCTCTAATCNAGGGACTGCNAGGAAATGGNGNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGCACTCACGGTGTGCATGTGGANNNNNNNNNNNNNNNNNNNNNNGTGTCTGAAATATNTCTTGTGTGTGCNAGGNG
7566
INCLUDED_REGION=23,443
7567
TARGET=340,48,GT
7568
TARGET=412,22,TG
7569
PRIMER_COMMENT=CL is AAACTATGGCTCGGTGGTTG
7570
PRIMER_COMMENT=CR is CACATGCACACCGTGAGTG
7571
PRIMER_PAIR_PENALTY=1.8499
7572
PRIMER_LEFT_PENALTY=0.006945
7573
PRIMER_RIGHT_PENALTY=1.842973
7574
PRIMER_LEFT_SEQUENCE=AAACTATGGCTCGGTGGTTG
7575
PRIMER_RIGHT_SEQUENCE=CACATGCACACCGTGAGTG
7576
PRIMER_LEFT=239,20
7577
PRIMER_RIGHT=409,19
7578
PRIMER_LEFT_TM=59.993
7579
PRIMER_RIGHT_TM=60.843
7580
PRIMER_LEFT_GC_PERCENT=50.000
7581
PRIMER_RIGHT_GC_PERCENT=57.895
7582
PRIMER_LEFT_SELF_ANY=3.00
7583
PRIMER_RIGHT_SELF_ANY=5.00
7584
PRIMER_LEFT_SELF_END=0.00
7585
PRIMER_RIGHT_SELF_END=4.00
7586
PRIMER_LEFT_END_STABILITY=8.2000
7587
PRIMER_RIGHT_END_STABILITY=6.4000
7588
PRIMER_PAIR_COMPL_ANY=5.00
7589
PRIMER_PAIR_COMPL_END=0.00
7590
PRIMER_PRODUCT_SIZE=171
7591
PRIMER_PAIR_PENALTY_1=1.8499
7592
PRIMER_LEFT_1_PENALTY=0.006945
7593
PRIMER_RIGHT_1_PENALTY=1.842973
7594
PRIMER_LEFT_1_SEQUENCE=CAAACTATGGCTCGGTGGTT
7595
PRIMER_RIGHT_1_SEQUENCE=CACATGCACACCGTGAGTG
7596
PRIMER_LEFT_1=238,20
7597
PRIMER_RIGHT_1=409,19
7598
PRIMER_LEFT_1_TM=59.993
7599
PRIMER_RIGHT_1_TM=60.843
7600
PRIMER_LEFT_1_GC_PERCENT=50.000
7601
PRIMER_RIGHT_1_GC_PERCENT=57.895
7602
PRIMER_LEFT_1_SELF_ANY=3.00
7603
PRIMER_RIGHT_1_SELF_ANY=5.00
7604
PRIMER_LEFT_1_SELF_END=2.00
7605
PRIMER_RIGHT_1_SELF_END=4.00
7606
PRIMER_LEFT_1_END_STABILITY=8.2000
7607
PRIMER_RIGHT_1_END_STABILITY=6.4000
7608
PRIMER_PAIR_1_COMPL_ANY=5.00
7609
PRIMER_PAIR_1_COMPL_END=0.00
7610
PRIMER_PRODUCT_SIZE_1=172
7611
PRIMER_PAIR_PENALTY_2=2.0565
7612
PRIMER_LEFT_2_PENALTY=0.213506
7613
PRIMER_RIGHT_2_PENALTY=1.842973
7614
PRIMER_LEFT_2_SEQUENCE=TATGGCTCGGTGGTTGGTAT
7615
PRIMER_RIGHT_2_SEQUENCE=CACATGCACACCGTGAGTG
7616
PRIMER_LEFT_2=243,20
7617
PRIMER_RIGHT_2=409,19
7618
PRIMER_LEFT_2_TM=60.214
7619
PRIMER_RIGHT_2_TM=60.843
7620
PRIMER_LEFT_2_GC_PERCENT=50.000
7621
PRIMER_RIGHT_2_GC_PERCENT=57.895
7622
PRIMER_LEFT_2_SELF_ANY=2.00
7623
PRIMER_RIGHT_2_SELF_ANY=5.00
7624
PRIMER_LEFT_2_SELF_END=2.00
7625
PRIMER_RIGHT_2_SELF_END=4.00
7626
PRIMER_LEFT_2_END_STABILITY=6.8000
7627
PRIMER_RIGHT_2_END_STABILITY=6.4000
7628
PRIMER_PAIR_2_COMPL_ANY=5.00
7629
PRIMER_PAIR_2_COMPL_END=0.00
7630
PRIMER_PRODUCT_SIZE_2=167
7631
PRIMER_PAIR_PENALTY_3=2.0565
7632
PRIMER_LEFT_3_PENALTY=0.213506
7633
PRIMER_RIGHT_3_PENALTY=1.842973
7634
PRIMER_LEFT_3_SEQUENCE=ATGGCTCGGTGGTTGGTATA
7635
PRIMER_RIGHT_3_SEQUENCE=CACATGCACACCGTGAGTG
7636
PRIMER_LEFT_3=244,20
7637
PRIMER_RIGHT_3=409,19
7638
PRIMER_LEFT_3_TM=60.214
7639
PRIMER_RIGHT_3_TM=60.843
7640
PRIMER_LEFT_3_GC_PERCENT=50.000
7641
PRIMER_RIGHT_3_GC_PERCENT=57.895
7642
PRIMER_LEFT_3_SELF_ANY=4.00
7643
PRIMER_RIGHT_3_SELF_ANY=5.00
7644
PRIMER_LEFT_3_SELF_END=4.00
7645
PRIMER_RIGHT_3_SELF_END=4.00
7646
PRIMER_LEFT_3_END_STABILITY=4.6000
7647
PRIMER_RIGHT_3_END_STABILITY=6.4000
7648
PRIMER_PAIR_3_COMPL_ANY=5.00
7649
PRIMER_PAIR_3_COMPL_END=0.00
7650
PRIMER_PRODUCT_SIZE_3=166
7651
PRIMER_PAIR_PENALTY_4=2.7216
7652
PRIMER_LEFT_4_PENALTY=0.006945
7653
PRIMER_RIGHT_4_PENALTY=2.714661
7654
PRIMER_LEFT_4_SEQUENCE=AAACTATGGCTCGGTGGTTG
7655
PRIMER_RIGHT_4_SEQUENCE=CCACATGCACACCGTGAGT
7656
PRIMER_LEFT_4=239,20
7657
PRIMER_RIGHT_4=410,19
7658
PRIMER_LEFT_4_TM=59.993
7659
PRIMER_RIGHT_4_TM=61.715
7660
PRIMER_LEFT_4_GC_PERCENT=50.000
7661
PRIMER_RIGHT_4_GC_PERCENT=57.895
7662
PRIMER_LEFT_4_SELF_ANY=3.00
7663
PRIMER_RIGHT_4_SELF_ANY=5.00
7664
PRIMER_LEFT_4_SELF_END=0.00
7665
PRIMER_RIGHT_4_SELF_END=3.00
7666
PRIMER_LEFT_4_END_STABILITY=8.2000
7667
PRIMER_RIGHT_4_END_STABILITY=6.4000
7668
PRIMER_PAIR_4_COMPL_ANY=5.00
7669
PRIMER_PAIR_4_COMPL_END=1.00
7670
PRIMER_PRODUCT_SIZE_4=172
7671
=
7672
MARKER_NAME=18.RHAP64FF9.seq
7673
SEQUENCE=NNNNNNNNNNNNNCCTTGGAAAATCACTGGGAAAGGGCTAAGTTGGCACGCCCCTTAGAGCAGGGAGGAACTNGGTGGTCGGCAGGGTCATTATGACATCTTGTGGTTGTTCTCAAGATTTACCACAGGGAGGGGTAGGGGGGTCTTTCCGAGAACAGTATCTTTGTTTTGTACTAACTTATGGTCAGAATAGTCTGGTCATTCTGACTACTANAACTTTGTCATAAATTAGGCAACCGGAAGTCAGATGTATTTCTCAAAACCTTGTTTTTACAACTTTGTTTCTTTGNNTCNCATATCTATGGAACTATGAAACCTTANTTCNTCAGNGGGGGGGGGGGGGAGAAAGACNNNNNNNNNNNNNNNNNNGAGNGGAGAGAGGANAGNGGANAGACNGANNNAGNNANGANAGACANAGACAGAGNACNGGAAAGAGNNNNNCAAGNNNCAGAAGGNGNGNNNNG
7674
INCLUDED_REGION=13,451
7675
TARGET=351,18,AG
7676
PRIMER_COMMENT=CL is
7677
PRIMER_COMMENT=CR is
7678
=
7679
MARKER_NAME=19.RHAP64FG1.seq
7680
SEQUENCE=NNNNNNNNNNNNNNNNNNCCAGAGAGTGATCTACCTACTGTTGGTTCCAGAGCCTGAGTCTGTTACAGCGGTCTGCACACACCACCATTCCCATTTCCAAGATAAGGAAANTGGGTCAAGGTNTTTGGGTGAGCGTGCGACAGATGAACTCATGGTCAAGGACTTCTTGGCTTTGAAAACCTGACCCTCACTCCCAAGTGTTCTCTGGGCTGGTTTGCCTCAGTTGGTTTTAACAGACTGGTCTAAAATTTCACCTCAAAATATAGAGCATGAAAGTAGAAATAAAGCTTCAATATCTGTCCAAATCTCATTTGTTCTATTTTTTATCATCATCATCATCACCACCACCACCATCATCATCATTACCACCACCATCATCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCACNATCACCANCACCATCATCACCACC
7681
INCLUDED_REGION=18,427
7682
TARGET=381,35,CAC
7683
PRIMER_COMMENT=CL is
7684
PRIMER_COMMENT=CR is
7685
=
7686
MARKER_NAME=21.RHAP64FG3.seq
7687
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCCAGCCAATCTAACCAGTTAACATGCTCCAGGTTCAGTGAGAGACTTTCAAAAGGAATAAAGCCATAGAGAGGGGCATCCAGTGTTGACCTATGGCATGNNNNNNNNNNNNNNGGCATGTGCACCCATGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAGCACTTCATTAGTCAAGTACAGCAGAGTGAACAGGCTAGAAGGTAACAGGTGACCACGTGTACCTCCCTCCACCCCATCCTCCTACCCCCACACACCCTCTGCAGGGAGCTGCCTGGAGAAACAGNAGAGCCTGTGGTAAGGTGTGCTGGGAGGGTGGAGAGTTAAGGAAAAGTGCAGAAAGCGATCTGTGTGGTTTCCACAGAAAGGNATGGCTGCCGTGGAATGTGTCCGGG
7688
INCLUDED_REGION=21,423
7689
TARGET=120,14,CA
7690
TARGET=152,55,CA
7691
PRIMER_COMMENT=CL is TCCAGTGTTGACCTATGGCA
7692
PRIMER_COMMENT=CR is CACGTGGTCACCTGTTACCTT
7693
PRIMER_PAIR_PENALTY=1.1738
7694
PRIMER_LEFT_PENALTY=0.112124
7695
PRIMER_RIGHT_PENALTY=1.061709
7696
PRIMER_LEFT_SEQUENCE=TCCAGTGTTGACCTATGGCA
7697
PRIMER_RIGHT_SEQUENCE=CACGTGGTCACCTGTTACCTT
7698
PRIMER_LEFT=98,20
7699
PRIMER_RIGHT=269,21
7700
PRIMER_LEFT_TM=60.112
7701
PRIMER_RIGHT_TM=59.938
7702
PRIMER_LEFT_GC_PERCENT=50.000
7703
PRIMER_RIGHT_GC_PERCENT=52.381
7704
PRIMER_LEFT_SELF_ANY=4.00
7705
PRIMER_RIGHT_SELF_ANY=6.00
7706
PRIMER_LEFT_SELF_END=4.00
7707
PRIMER_RIGHT_SELF_END=2.00
7708
PRIMER_LEFT_END_STABILITY=10.0000
7709
PRIMER_RIGHT_END_STABILITY=7.9000
7710
PRIMER_PAIR_COMPL_ANY=5.00
7711
PRIMER_PAIR_COMPL_END=2.00
7712
PRIMER_PRODUCT_SIZE=172
7713
PRIMER_PAIR_PENALTY_1=1.6212
7714
PRIMER_LEFT_1_PENALTY=0.112124
7715
PRIMER_RIGHT_1_PENALTY=1.509026
7716
PRIMER_LEFT_1_SEQUENCE=TCCAGTGTTGACCTATGGCA
7717
PRIMER_RIGHT_1_SEQUENCE=CACGTGGTCACCTGTTACCT
7718
PRIMER_LEFT_1=98,20
7719
PRIMER_RIGHT_1=269,20
7720
PRIMER_LEFT_1_TM=60.112
7721
PRIMER_RIGHT_1_TM=58.491
7722
PRIMER_LEFT_1_GC_PERCENT=50.000
7723
PRIMER_RIGHT_1_GC_PERCENT=55.000
7724
PRIMER_LEFT_1_SELF_ANY=4.00
7725
PRIMER_RIGHT_1_SELF_ANY=6.00
7726
PRIMER_LEFT_1_SELF_END=4.00
7727
PRIMER_RIGHT_1_SELF_END=4.00
7728
PRIMER_LEFT_1_END_STABILITY=10.0000
7729
PRIMER_RIGHT_1_END_STABILITY=6.9000
7730
PRIMER_PAIR_1_COMPL_ANY=5.00
7731
PRIMER_PAIR_1_COMPL_END=1.00
7732
PRIMER_PRODUCT_SIZE_1=172
7733
PRIMER_PAIR_PENALTY_2=1.7021
7734
PRIMER_LEFT_2_PENALTY=0.112124
7735
PRIMER_RIGHT_2_PENALTY=1.590010
7736
PRIMER_LEFT_2_SEQUENCE=TCCAGTGTTGACCTATGGCA
7737
PRIMER_RIGHT_2_SEQUENCE=ACACGTGGTCACCTGTTACCT
7738
PRIMER_LEFT_2=98,20
7739
PRIMER_RIGHT_2=270,21
7740
PRIMER_LEFT_2_TM=60.112
7741
PRIMER_RIGHT_2_TM=59.410
7742
PRIMER_LEFT_2_GC_PERCENT=50.000
7743
PRIMER_RIGHT_2_GC_PERCENT=52.381
7744
PRIMER_LEFT_2_SELF_ANY=4.00
7745
PRIMER_RIGHT_2_SELF_ANY=8.00
7746
PRIMER_LEFT_2_SELF_END=4.00
7747
PRIMER_RIGHT_2_SELF_END=4.00
7748
PRIMER_LEFT_2_END_STABILITY=10.0000
7749
PRIMER_RIGHT_2_END_STABILITY=6.9000
7750
PRIMER_PAIR_2_COMPL_ANY=5.00
7751
PRIMER_PAIR_2_COMPL_END=1.00
7752
PRIMER_PRODUCT_SIZE_2=173
7753
PRIMER_PAIR_PENALTY_3=1.7793
7754
PRIMER_LEFT_3_PENALTY=0.112124
7755
PRIMER_RIGHT_3_PENALTY=1.667221
7756
PRIMER_LEFT_3_SEQUENCE=TCCAGTGTTGACCTATGGCA
7757
PRIMER_RIGHT_3_SEQUENCE=ACACGTGGTCACCTGTTACC
7758
PRIMER_LEFT_3=98,20
7759
PRIMER_RIGHT_3=270,20
7760
PRIMER_LEFT_3_TM=60.112
7761
PRIMER_RIGHT_3_TM=58.333
7762
PRIMER_LEFT_3_GC_PERCENT=50.000
7763
PRIMER_RIGHT_3_GC_PERCENT=55.000
7764
PRIMER_LEFT_3_SELF_ANY=4.00
7765
PRIMER_RIGHT_3_SELF_ANY=8.00
7766
PRIMER_LEFT_3_SELF_END=4.00
7767
PRIMER_RIGHT_3_SELF_END=6.00
7768
PRIMER_LEFT_3_END_STABILITY=10.0000
7769
PRIMER_RIGHT_3_END_STABILITY=7.2000
7770
PRIMER_PAIR_3_COMPL_ANY=5.00
7771
PRIMER_PAIR_3_COMPL_END=2.00
7772
PRIMER_PRODUCT_SIZE_3=173
7773
PRIMER_PAIR_PENALTY_4=1.9899
7774
PRIMER_LEFT_4_PENALTY=0.112124
7775
PRIMER_RIGHT_4_PENALTY=1.877751
7776
PRIMER_LEFT_4_SEQUENCE=TCCAGTGTTGACCTATGGCA
7777
PRIMER_RIGHT_4_SEQUENCE=CGTGGTCACCTGTTACCTTCT
7778
PRIMER_LEFT_4=98,20
7779
PRIMER_RIGHT_4=267,21
7780
PRIMER_LEFT_4_TM=60.112
7781
PRIMER_RIGHT_4_TM=59.122
7782
PRIMER_LEFT_4_GC_PERCENT=50.000
7783
PRIMER_RIGHT_4_GC_PERCENT=52.381
7784
PRIMER_LEFT_4_SELF_ANY=4.00
7785
PRIMER_RIGHT_4_SELF_ANY=6.00
7786
PRIMER_LEFT_4_SELF_END=4.00
7787
PRIMER_RIGHT_4_SELF_END=0.00
7788
PRIMER_LEFT_4_END_STABILITY=10.0000
7789
PRIMER_RIGHT_4_END_STABILITY=6.7000
7790
PRIMER_PAIR_4_COMPL_ANY=5.00
7791
PRIMER_PAIR_4_COMPL_END=1.00
7792
PRIMER_PRODUCT_SIZE_4=170
7793
=
7794
MARKER_NAME=22.RHAP64FH7.seq
7795
SEQUENCE=NNNNNNNNNNNNCCACCCCTTGTTGTCTTTACTATGNGCCTCTATATGACTATACGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNTNNNTCNCTCTCNTNNNNTNNANANANANNNNNNNNNNNNNNNNNNNNNTGNGNNGNNNGTGTGNGNGTNNNTCNNTCNNATNCNNNANNGCCANNTGGANTANNNNANNNTCNTNNNAANTAGANNNGGGNNNTNGNANTNNCCNGGAGGTNTCNCANCCACNNCTCNGCANANGNACANNTANNCNNCAGNACTCACATNCTNNGANANACNNNTNTGTTCTGGNNNCCANTNNNAANGNTNNNTTNNNNATAATNNNNNGNNCTCNNCANNGTGNNNNTCNNACTCCNNNNTANGNNTNNNGNTTNCAGNNGNANNNNNAGTANANGNNACNTCNNGNNTNNTGATGGNNNNNNNNNTNTNNGNNNCCNNNTNCNGANNTNNNNNNNNTNG
7796
INCLUDED_REGION=12,488
7797
TARGET=57,22,CT
7798
TARGET=137,18,GT
7799
PRIMER_COMMENT=CL is
7800
PRIMER_COMMENT=CR is
7801
=
7802
MARKER_NAME=26.RHAP64FA11.seq
7803
SEQUENCE=NNNNNNNNNNNNCATCCTACTAAGGGAAAGCAAAAAAAAAACTTCCTCCTGACTNTGATGTTGAAAGAAGAATGAAACGAACATCTCTCCAACAATATCTAATCAGCAGGAGTCATACTCACCCTTAGGTCTAGATTTACACTTTTCCAAGATCTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGTGGGGGGGTGGAGAGGAAGAAGGACAGAGACACAGAGAGATCACTAGACATATAATACACTACGAATTGTACAATTAAGAATTCAAAAAATTAAAAAAAAAACCATTTCTGATAGCCAGACAAGTCAGTTTGATGAACAAAGAGGAAGAATGTGAGTGAGGAAGGATGNAATTGGAGAGAGGGATTGAAAATGGCAGTANTGTTAAAGATTGGAGAAGAAGGAAGTCCTGTGGGAGAGGGGGTGGGGGAGA
7804
INCLUDED_REGION=12,445
7805
TARGET=157,46,AC
7806
PRIMER_COMMENT=CL is CAGCAGGAGTCATACTCACCC
7807
PRIMER_COMMENT=CR is GTCCTTCTTCCTCTCCACCC
7808
PRIMER_PAIR_PENALTY=1.3143
7809
PRIMER_LEFT_PENALTY=1.263803
7810
PRIMER_RIGHT_PENALTY=0.050466
7811
PRIMER_LEFT_SEQUENCE=CAGCAGGAGTCATACTCACCC
7812
PRIMER_RIGHT_SEQUENCE=GTCCTTCTTCCTCTCCACCC
7813
PRIMER_LEFT=103,21
7814
PRIMER_RIGHT=230,20
7815
PRIMER_LEFT_TM=59.736
7816
PRIMER_RIGHT_TM=60.050
7817
PRIMER_LEFT_GC_PERCENT=57.143
7818
PRIMER_RIGHT_GC_PERCENT=60.000
7819
PRIMER_LEFT_SELF_ANY=6.00
7820
PRIMER_RIGHT_SELF_ANY=2.00
7821
PRIMER_LEFT_SELF_END=2.00
7822
PRIMER_RIGHT_SELF_END=0.00
7823
PRIMER_LEFT_END_STABILITY=9.4000
7824
PRIMER_RIGHT_END_STABILITY=9.4000
7825
PRIMER_PAIR_COMPL_ANY=5.00
7826
PRIMER_PAIR_COMPL_END=0.00
7827
PRIMER_PRODUCT_SIZE=128
7828
PRIMER_PAIR_PENALTY_1=2.0359
7829
PRIMER_LEFT_1_PENALTY=1.263803
7830
PRIMER_RIGHT_1_PENALTY=0.772097
7831
PRIMER_LEFT_1_SEQUENCE=CAGCAGGAGTCATACTCACCC
7832
PRIMER_RIGHT_1_SEQUENCE=TGTCCTTCTTCCTCTCCACC
7833
PRIMER_LEFT_1=103,21
7834
PRIMER_RIGHT_1=231,20
7835
PRIMER_LEFT_1_TM=59.736
7836
PRIMER_RIGHT_1_TM=59.228
7837
PRIMER_LEFT_1_GC_PERCENT=57.143
7838
PRIMER_RIGHT_1_GC_PERCENT=55.000
7839
PRIMER_LEFT_1_SELF_ANY=6.00
7840
PRIMER_RIGHT_1_SELF_ANY=2.00
7841
PRIMER_LEFT_1_SELF_END=2.00
7842
PRIMER_RIGHT_1_SELF_END=0.00
7843
PRIMER_LEFT_1_END_STABILITY=9.4000
7844
PRIMER_RIGHT_1_END_STABILITY=9.4000
7845
PRIMER_PAIR_1_COMPL_ANY=5.00
7846
PRIMER_PAIR_1_COMPL_END=0.00
7847
PRIMER_PRODUCT_SIZE_1=129
7848
PRIMER_PAIR_PENALTY_2=2.5011
7849
PRIMER_LEFT_2_PENALTY=1.263803
7850
PRIMER_RIGHT_2_PENALTY=1.237277
7851
PRIMER_LEFT_2_SEQUENCE=CAGCAGGAGTCATACTCACCC
7852
PRIMER_RIGHT_2_SEQUENCE=CTGTCCTTCTTCCTCTCCACC
7853
PRIMER_LEFT_2=103,21
7854
PRIMER_RIGHT_2=232,21
7855
PRIMER_LEFT_2_TM=59.736
7856
PRIMER_RIGHT_2_TM=60.237
7857
PRIMER_LEFT_2_GC_PERCENT=57.143
7858
PRIMER_RIGHT_2_GC_PERCENT=57.143
7859
PRIMER_LEFT_2_SELF_ANY=6.00
7860
PRIMER_RIGHT_2_SELF_ANY=2.00
7861
PRIMER_LEFT_2_SELF_END=2.00
7862
PRIMER_RIGHT_2_SELF_END=0.00
7863
PRIMER_LEFT_2_END_STABILITY=9.4000
7864
PRIMER_RIGHT_2_END_STABILITY=9.4000
7865
PRIMER_PAIR_2_COMPL_ANY=5.00
7866
PRIMER_PAIR_2_COMPL_END=0.00
7867
PRIMER_PRODUCT_SIZE_2=130
7868
PRIMER_PAIR_PENALTY_3=2.7188
7869
PRIMER_LEFT_3_PENALTY=2.668315
7870
PRIMER_RIGHT_3_PENALTY=0.050466
7871
PRIMER_LEFT_3_SEQUENCE=TGAAACGAACATCTCTCCAACA
7872
PRIMER_RIGHT_3_SEQUENCE=GTCCTTCTTCCTCTCCACCC
7873
PRIMER_LEFT_3=72,22
7874
PRIMER_RIGHT_3=230,20
7875
PRIMER_LEFT_3_TM=60.668
7876
PRIMER_RIGHT_3_TM=60.050
7877
PRIMER_LEFT_3_GC_PERCENT=40.909
7878
PRIMER_RIGHT_3_GC_PERCENT=60.000
7879
PRIMER_LEFT_3_SELF_ANY=2.00
7880
PRIMER_RIGHT_3_SELF_ANY=2.00
7881
PRIMER_LEFT_3_SELF_END=0.00
7882
PRIMER_RIGHT_3_SELF_END=0.00
7883
PRIMER_LEFT_3_END_STABILITY=7.0000
7884
PRIMER_RIGHT_3_END_STABILITY=9.4000
7885
PRIMER_PAIR_3_COMPL_ANY=4.00
7886
PRIMER_PAIR_3_COMPL_END=0.00
7887
PRIMER_PRODUCT_SIZE_3=159
7888
PRIMER_PAIR_PENALTY_4=2.7516
7889
PRIMER_LEFT_4_PENALTY=2.701125
7890
PRIMER_RIGHT_4_PENALTY=0.050466
7891
PRIMER_LEFT_4_SEQUENCE=CAGCAGGAGTCATACTCACCCT
7892
PRIMER_RIGHT_4_SEQUENCE=GTCCTTCTTCCTCTCCACCC
7893
PRIMER_LEFT_4=103,22
7894
PRIMER_RIGHT_4=230,20
7895
PRIMER_LEFT_4_TM=60.701
7896
PRIMER_RIGHT_4_TM=60.050
7897
PRIMER_LEFT_4_GC_PERCENT=54.545
7898
PRIMER_RIGHT_4_GC_PERCENT=60.000
7899
PRIMER_LEFT_4_SELF_ANY=6.00
7900
PRIMER_RIGHT_4_SELF_ANY=2.00
7901
PRIMER_LEFT_4_SELF_END=4.00
7902
PRIMER_RIGHT_4_SELF_END=0.00
7903
PRIMER_LEFT_4_END_STABILITY=9.1000
7904
PRIMER_RIGHT_4_END_STABILITY=9.4000
7905
PRIMER_PAIR_4_COMPL_ANY=5.00
7906
PRIMER_PAIR_4_COMPL_END=0.00
7907
PRIMER_PRODUCT_SIZE_4=128
7908
=
7909
MARKER_NAME=30.RHAP64FB12.seq
7910
SEQUENCE=CCTTCCTATTTTCCTCTCCAGTGCTGAGACCAAAGTGAGATCAAAGTCATGCATNACCATGGGNACCTTCCTTTTCANNANTTTNTTTCATCNTAGGAAGTANGTANANANGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNGCTTTAAAATCAGCACTCAAGANNTNTNNGNTTCCTATCCCTATANCANNCCCCCCAAAACANCCNNAAAGCCACCNGCNCCAGAGAAAGAGGGAGATGAAATCTCTAGTNNGAAAAANNAGNNGAGGGAGATNACAGNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNGNGNANANACACAGATACANNNCCNNANANNNNGGNGANACNNNNNNNNNGNNGCNNGCGNGCNNNGAGTANTNNANAAANANNGNTNCTNNNNGNGNNNCNNGGNGNNNA
7911
INCLUDED_REGION=0,451
7912
TARGET=113,32,GT
7913
TARGET=146,26,GA
7914
TARGET=314,24,CT
7915
PRIMER_COMMENT=CL is TCTCCAGTGCTGAGACCAAA
7916
PRIMER_COMMENT=CR is TTTCATCTCCCTCTTTCTCTGG
7917
PRIMER_PAIR_PENALTY=2.6340
7918
PRIMER_LEFT_PENALTY=0.454603
7919
PRIMER_RIGHT_PENALTY=2.179421
7920
PRIMER_LEFT_SEQUENCE=TCTCCAGTGCTGAGACCAAA
7921
PRIMER_RIGHT_SEQUENCE=TTTCATCTCCCTCTTTCTCTGG
7922
PRIMER_LEFT=14,20
7923
PRIMER_RIGHT=273,22
7924
PRIMER_LEFT_TM=59.545
7925
PRIMER_RIGHT_TM=59.821
7926
PRIMER_LEFT_GC_PERCENT=50.000
7927
PRIMER_RIGHT_GC_PERCENT=45.455
7928
PRIMER_LEFT_SELF_ANY=6.00
7929
PRIMER_RIGHT_SELF_ANY=2.00
7930
PRIMER_LEFT_SELF_END=0.00
7931
PRIMER_RIGHT_SELF_END=0.00
7932
PRIMER_LEFT_END_STABILITY=8.8000
7933
PRIMER_RIGHT_END_STABILITY=8.2000
7934
PRIMER_PAIR_COMPL_ANY=5.00
7935
PRIMER_PAIR_COMPL_END=3.00
7936
PRIMER_PRODUCT_SIZE=260
7937
PRIMER_PAIR_PENALTY_1=2.7181
7938
PRIMER_LEFT_1_PENALTY=0.538636
7939
PRIMER_RIGHT_1_PENALTY=2.179421
7940
PRIMER_LEFT_1_SEQUENCE=CCAGTGCTGAGACCAAAGTG
7941
PRIMER_RIGHT_1_SEQUENCE=TTTCATCTCCCTCTTTCTCTGG
7942
PRIMER_LEFT_1=17,20
7943
PRIMER_RIGHT_1=273,22
7944
PRIMER_LEFT_1_TM=59.461
7945
PRIMER_RIGHT_1_TM=59.821
7946
PRIMER_LEFT_1_GC_PERCENT=55.000
7947
PRIMER_RIGHT_1_GC_PERCENT=45.455
7948
PRIMER_LEFT_1_SELF_ANY=4.00
7949
PRIMER_RIGHT_1_SELF_ANY=2.00
7950
PRIMER_LEFT_1_SELF_END=1.00
7951
PRIMER_RIGHT_1_SELF_END=0.00
7952
PRIMER_LEFT_1_END_STABILITY=6.7000
7953
PRIMER_RIGHT_1_END_STABILITY=8.2000
7954
PRIMER_PAIR_1_COMPL_ANY=5.00
7955
PRIMER_PAIR_1_COMPL_END=2.00
7956
PRIMER_PRODUCT_SIZE_1=257
7957
PRIMER_PAIR_PENALTY_2=2.7292
7958
PRIMER_LEFT_2_PENALTY=0.549770
7959
PRIMER_RIGHT_2_PENALTY=2.179421
7960
PRIMER_LEFT_2_SEQUENCE=TCCTCTCCAGTGCTGAGACC
7961
PRIMER_RIGHT_2_SEQUENCE=TTTCATCTCCCTCTTTCTCTGG
7962
PRIMER_LEFT_2=11,20
7963
PRIMER_RIGHT_2=273,22
7964
PRIMER_LEFT_2_TM=60.550
7965
PRIMER_RIGHT_2_TM=59.821
7966
PRIMER_LEFT_2_GC_PERCENT=60.000
7967
PRIMER_RIGHT_2_GC_PERCENT=45.455
7968
PRIMER_LEFT_2_SELF_ANY=6.00
7969
PRIMER_RIGHT_2_SELF_ANY=2.00
7970
PRIMER_LEFT_2_SELF_END=2.00
7971
PRIMER_RIGHT_2_SELF_END=0.00
7972
PRIMER_LEFT_2_END_STABILITY=7.6000
7973
PRIMER_RIGHT_2_END_STABILITY=8.2000
7974
PRIMER_PAIR_2_COMPL_ANY=4.00
7975
PRIMER_PAIR_2_COMPL_END=2.00
7976
PRIMER_PRODUCT_SIZE_2=263
7977
PRIMER_PAIR_PENALTY_3=2.9960
7978
PRIMER_LEFT_3_PENALTY=0.454603
7979
PRIMER_RIGHT_3_PENALTY=2.541433
7980
PRIMER_LEFT_3_SEQUENCE=TCTCCAGTGCTGAGACCAAA
7981
PRIMER_RIGHT_3_SEQUENCE=TTCATCTCCCTCTTTCTCTGG
7982
PRIMER_LEFT_3=14,20
7983
PRIMER_RIGHT_3=272,21
7984
PRIMER_LEFT_3_TM=59.545
7985
PRIMER_RIGHT_3_TM=58.459
7986
PRIMER_LEFT_3_GC_PERCENT=50.000
7987
PRIMER_RIGHT_3_GC_PERCENT=47.619
7988
PRIMER_LEFT_3_SELF_ANY=6.00
7989
PRIMER_RIGHT_3_SELF_ANY=2.00
7990
PRIMER_LEFT_3_SELF_END=0.00
7991
PRIMER_RIGHT_3_SELF_END=0.00
7992
PRIMER_LEFT_3_END_STABILITY=8.8000
7993
PRIMER_RIGHT_3_END_STABILITY=8.2000
7994
PRIMER_PAIR_3_COMPL_ANY=4.00
7995
PRIMER_PAIR_3_COMPL_END=3.00
7996
PRIMER_PRODUCT_SIZE_3=259
7997
PRIMER_PAIR_PENALTY_4=3.0549
7998
PRIMER_LEFT_4_PENALTY=0.875462
7999
PRIMER_RIGHT_4_PENALTY=2.179421
8000
PRIMER_LEFT_4_SEQUENCE=CTCTCCAGTGCTGAGACCAA
8001
PRIMER_RIGHT_4_SEQUENCE=TTTCATCTCCCTCTTTCTCTGG
8002
PRIMER_LEFT_4=13,20
8003
PRIMER_RIGHT_4=273,22
8004
PRIMER_LEFT_4_TM=59.125
8005
PRIMER_RIGHT_4_TM=59.821
8006
PRIMER_LEFT_4_GC_PERCENT=55.000
8007
PRIMER_RIGHT_4_GC_PERCENT=45.455
8008
PRIMER_LEFT_4_SELF_ANY=6.00
8009
PRIMER_RIGHT_4_SELF_ANY=2.00
8010
PRIMER_LEFT_4_SELF_END=1.00
8011
PRIMER_RIGHT_4_SELF_END=0.00
8012
PRIMER_LEFT_4_END_STABILITY=8.2000
8013
PRIMER_RIGHT_4_END_STABILITY=8.2000
8014
PRIMER_PAIR_4_COMPL_ANY=4.00
8015
PRIMER_PAIR_4_COMPL_END=2.00
8016
PRIMER_PRODUCT_SIZE_4=261
8017
=
8018
MARKER_NAME=31.RHAP64FC10.seq
8019
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNCCTAACCCTTTATGCTATCTAGTTACTTCCCAGACATGCACACACGCACGCACGCACGCACGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATGCCCCAAGTTACTTGCATTTAGTCTCACCTTGGATACATTCAGCTGTGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
8020
INCLUDED_REGION=24,167
8021
TARGET=86,52,CA
8022
PRIMER_COMMENT=CL is CTTCCCAGACATGCACACAC
8023
PRIMER_COMMENT=CR is CCCACAGCTGAATGTATCCA
8024
PRIMER_PAIR_PENALTY=0.6410
8025
PRIMER_LEFT_PENALTY=0.162457
8026
PRIMER_RIGHT_PENALTY=0.478496
8027
PRIMER_LEFT_SEQUENCE=CTTCCCAGACATGCACACAC
8028
PRIMER_RIGHT_SEQUENCE=CCCACAGCTGAATGTATCCA
8029
PRIMER_LEFT=49,20
8030
PRIMER_RIGHT=190,20
8031
PRIMER_LEFT_TM=60.162
8032
PRIMER_RIGHT_TM=59.522
8033
PRIMER_LEFT_GC_PERCENT=55.000
8034
PRIMER_RIGHT_GC_PERCENT=50.000
8035
PRIMER_LEFT_SELF_ANY=4.00
8036
PRIMER_RIGHT_SELF_ANY=6.00
8037
PRIMER_LEFT_SELF_END=0.00
8038
PRIMER_RIGHT_SELF_END=4.00
8039
PRIMER_LEFT_END_STABILITY=6.4000
8040
PRIMER_RIGHT_END_STABILITY=8.1000
8041
PRIMER_PAIR_COMPL_ANY=5.00
8042
PRIMER_PAIR_COMPL_END=3.00
8043
PRIMER_PRODUCT_SIZE=142
8044
PRIMER_PAIR_PENALTY_1=0.6410
8045
PRIMER_LEFT_1_PENALTY=0.162457
8046
PRIMER_RIGHT_1_PENALTY=0.478496
8047
PRIMER_LEFT_1_SEQUENCE=ACTTCCCAGACATGCACACA
8048
PRIMER_RIGHT_1_SEQUENCE=CCCACAGCTGAATGTATCCA
8049
PRIMER_LEFT_1=48,20
8050
PRIMER_RIGHT_1=190,20
8051
PRIMER_LEFT_1_TM=60.162
8052
PRIMER_RIGHT_1_TM=59.522
8053
PRIMER_LEFT_1_GC_PERCENT=50.000
8054
PRIMER_RIGHT_1_GC_PERCENT=50.000
8055
PRIMER_LEFT_1_SELF_ANY=4.00
8056
PRIMER_RIGHT_1_SELF_ANY=6.00
8057
PRIMER_LEFT_1_SELF_END=0.00
8058
PRIMER_RIGHT_1_SELF_END=4.00
8059
PRIMER_LEFT_1_END_STABILITY=6.4000
8060
PRIMER_RIGHT_1_END_STABILITY=8.1000
8061
PRIMER_PAIR_1_COMPL_ANY=5.00
8062
PRIMER_PAIR_1_COMPL_END=4.00
8063
PRIMER_PRODUCT_SIZE_1=143
8064
PRIMER_PAIR_PENALTY_2=1.6459
8065
PRIMER_LEFT_2_PENALTY=1.167443
8066
PRIMER_RIGHT_2_PENALTY=0.478496
8067
PRIMER_LEFT_2_SEQUENCE=TACTTCCCAGACATGCACACA
8068
PRIMER_RIGHT_2_SEQUENCE=CCCACAGCTGAATGTATCCA
8069
PRIMER_LEFT_2=47,21
8070
PRIMER_RIGHT_2=190,20
8071
PRIMER_LEFT_2_TM=60.167
8072
PRIMER_RIGHT_2_TM=59.522
8073
PRIMER_LEFT_2_GC_PERCENT=47.619
8074
PRIMER_RIGHT_2_GC_PERCENT=50.000
8075
PRIMER_LEFT_2_SELF_ANY=4.00
8076
PRIMER_RIGHT_2_SELF_ANY=6.00
8077
PRIMER_LEFT_2_SELF_END=0.00
8078
PRIMER_RIGHT_2_SELF_END=4.00
8079
PRIMER_LEFT_2_END_STABILITY=6.4000
8080
PRIMER_RIGHT_2_END_STABILITY=8.1000
8081
PRIMER_PAIR_2_COMPL_ANY=5.00
8082
PRIMER_PAIR_2_COMPL_END=4.00
8083
PRIMER_PRODUCT_SIZE_2=144
8084
PRIMER_PAIR_PENALTY_3=2.0613
8085
PRIMER_LEFT_3_PENALTY=0.162457
8086
PRIMER_RIGHT_3_PENALTY=1.898804
8087
PRIMER_LEFT_3_SEQUENCE=CTTCCCAGACATGCACACAC
8088
PRIMER_RIGHT_3_SEQUENCE=CCCACAGCTGAATGTATCCAA
8089
PRIMER_LEFT_3=49,20
8090
PRIMER_RIGHT_3=190,21
8091
PRIMER_LEFT_3_TM=60.162
8092
PRIMER_RIGHT_3_TM=60.899
8093
PRIMER_LEFT_3_GC_PERCENT=55.000
8094
PRIMER_RIGHT_3_GC_PERCENT=47.619
8095
PRIMER_LEFT_3_SELF_ANY=4.00
8096
PRIMER_RIGHT_3_SELF_ANY=6.00
8097
PRIMER_LEFT_3_SELF_END=0.00
8098
PRIMER_RIGHT_3_SELF_END=2.00
8099
PRIMER_LEFT_3_END_STABILITY=6.4000
8100
PRIMER_RIGHT_3_END_STABILITY=8.5000
8101
PRIMER_PAIR_3_COMPL_ANY=5.00
8102
PRIMER_PAIR_3_COMPL_END=2.00
8103
PRIMER_PRODUCT_SIZE_3=142
8104
PRIMER_PAIR_PENALTY_4=2.0613
8105
PRIMER_LEFT_4_PENALTY=0.162457
8106
PRIMER_RIGHT_4_PENALTY=1.898804
8107
PRIMER_LEFT_4_SEQUENCE=ACTTCCCAGACATGCACACA
8108
PRIMER_RIGHT_4_SEQUENCE=CCCACAGCTGAATGTATCCAA
8109
PRIMER_LEFT_4=48,20
8110
PRIMER_RIGHT_4=190,21
8111
PRIMER_LEFT_4_TM=60.162
8112
PRIMER_RIGHT_4_TM=60.899
8113
PRIMER_LEFT_4_GC_PERCENT=50.000
8114
PRIMER_RIGHT_4_GC_PERCENT=47.619
8115
PRIMER_LEFT_4_SELF_ANY=4.00
8116
PRIMER_RIGHT_4_SELF_ANY=6.00
8117
PRIMER_LEFT_4_SELF_END=0.00
8118
PRIMER_RIGHT_4_SELF_END=2.00
8119
PRIMER_LEFT_4_END_STABILITY=6.4000
8120
PRIMER_RIGHT_4_END_STABILITY=8.5000
8121
PRIMER_PAIR_4_COMPL_ANY=5.00
8122
PRIMER_PAIR_4_COMPL_END=3.00
8123
PRIMER_PRODUCT_SIZE_4=143
8124
=
8125
MARKER_NAME=31.RHAP64FC4.seq
8126
SEQUENCE=NNNNNNNNNNNNNNNNNNNNCCTGTTGGAGCCCAGCAAAGATGTTTTCTAGAATTCCCAACTCAGACAATCCTTGGAGGTTGTTATGTGAATGTATATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATACACAGGCTCATGCCTTTGTGTGGAGGTCAGAGGCATCATCTAGCTTACATTTATTAAGACAGCGTTTCACATTTTGCTTGGAATTCATTGATTCAGCTGGACTGGCTGTTTGGAGAGCCTCAGGGATCCATCCACCTGCCTCTGTCCCCTTGACCCTGGGATTACAAGCAGTCATACCCAAACTCAGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAAACTCAGAACTTCATCTTGTGTAGCAACTGTTTTTCTAAGCGAGATGTCT
8127
INCLUDED_REGION=20,416
8128
TARGET=99,48,TG
8129
TARGET=340,44,TG
8130
PRIMER_COMMENT=CL is AGCCCAGCAAAGATGTTTTC
8131
PRIMER_COMMENT=CR is ATGATGCCTCTGACCTCCAC
8132
PRIMER_PAIR_PENALTY=0.7638
8133
PRIMER_LEFT_PENALTY=0.683672
8134
PRIMER_RIGHT_PENALTY=0.080144
8135
PRIMER_LEFT_SEQUENCE=GAGCCCAGCAAAGATGTTTT
8136
PRIMER_RIGHT_SEQUENCE=ATGATGCCTCTGACCTCCAC
8137
PRIMER_LEFT=27,20
8138
PRIMER_RIGHT=189,20
8139
PRIMER_LEFT_TM=59.316
8140
PRIMER_RIGHT_TM=60.080
8141
PRIMER_LEFT_GC_PERCENT=45.000
8142
PRIMER_RIGHT_GC_PERCENT=55.000
8143
PRIMER_LEFT_SELF_ANY=6.00
8144
PRIMER_RIGHT_SELF_ANY=4.00
8145
PRIMER_LEFT_SELF_END=4.00
8146
PRIMER_RIGHT_SELF_END=0.00
8147
PRIMER_LEFT_END_STABILITY=7.0000
8148
PRIMER_RIGHT_END_STABILITY=7.9000
8149
PRIMER_PAIR_COMPL_ANY=4.00
8150
PRIMER_PAIR_COMPL_END=0.00
8151
PRIMER_PRODUCT_SIZE=163
8152
PRIMER_PAIR_PENALTY_1=0.7638
8153
PRIMER_LEFT_1_PENALTY=0.683672
8154
PRIMER_RIGHT_1_PENALTY=0.080144
8155
PRIMER_LEFT_1_SEQUENCE=AGCCCAGCAAAGATGTTTTC
8156
PRIMER_RIGHT_1_SEQUENCE=ATGATGCCTCTGACCTCCAC
8157
PRIMER_LEFT_1=28,20
8158
PRIMER_RIGHT_1=189,20
8159
PRIMER_LEFT_1_TM=59.316
8160
PRIMER_RIGHT_1_TM=60.080
8161
PRIMER_LEFT_1_GC_PERCENT=45.000
8162
PRIMER_RIGHT_1_GC_PERCENT=55.000
8163
PRIMER_LEFT_1_SELF_ANY=6.00
8164
PRIMER_RIGHT_1_SELF_ANY=4.00
8165
PRIMER_LEFT_1_SELF_END=6.00
8166
PRIMER_RIGHT_1_SELF_END=0.00
8167
PRIMER_LEFT_1_END_STABILITY=7.3000
8168
PRIMER_RIGHT_1_END_STABILITY=7.9000
8169
PRIMER_PAIR_1_COMPL_ANY=3.00
8170
PRIMER_PAIR_1_COMPL_END=0.00
8171
PRIMER_PRODUCT_SIZE_1=162
8172
PRIMER_PAIR_PENALTY_2=0.8196
8173
PRIMER_LEFT_2_PENALTY=0.683672
8174
PRIMER_RIGHT_2_PENALTY=0.135900
8175
PRIMER_LEFT_2_SEQUENCE=GAGCCCAGCAAAGATGTTTT
8176
PRIMER_RIGHT_2_SEQUENCE=CAAAGGCATGAGCCTGTGTA
8177
PRIMER_LEFT_2=27,20
8178
PRIMER_RIGHT_2=168,20
8179
PRIMER_LEFT_2_TM=59.316
8180
PRIMER_RIGHT_2_TM=59.864
8181
PRIMER_LEFT_2_GC_PERCENT=45.000
8182
PRIMER_RIGHT_2_GC_PERCENT=50.000
8183
PRIMER_LEFT_2_SELF_ANY=6.00
8184
PRIMER_RIGHT_2_SELF_ANY=7.00
8185
PRIMER_LEFT_2_SELF_END=4.00
8186
PRIMER_RIGHT_2_SELF_END=3.00
8187
PRIMER_LEFT_2_END_STABILITY=7.0000
8188
PRIMER_RIGHT_2_END_STABILITY=5.4000
8189
PRIMER_PAIR_2_COMPL_ANY=3.00
8190
PRIMER_PAIR_2_COMPL_END=1.00
8191
PRIMER_PRODUCT_SIZE_2=142
8192
PRIMER_PAIR_PENALTY_3=0.8196
8193
PRIMER_LEFT_3_PENALTY=0.683672
8194
PRIMER_RIGHT_3_PENALTY=0.135900
8195
PRIMER_LEFT_3_SEQUENCE=AGCCCAGCAAAGATGTTTTC
8196
PRIMER_RIGHT_3_SEQUENCE=CAAAGGCATGAGCCTGTGTA
8197
PRIMER_LEFT_3=28,20
8198
PRIMER_RIGHT_3=168,20
8199
PRIMER_LEFT_3_TM=59.316
8200
PRIMER_RIGHT_3_TM=59.864
8201
PRIMER_LEFT_3_GC_PERCENT=45.000
8202
PRIMER_RIGHT_3_GC_PERCENT=50.000
8203
PRIMER_LEFT_3_SELF_ANY=6.00
8204
PRIMER_RIGHT_3_SELF_ANY=7.00
8205
PRIMER_LEFT_3_SELF_END=6.00
8206
PRIMER_RIGHT_3_SELF_END=3.00
8207
PRIMER_LEFT_3_END_STABILITY=7.3000
8208
PRIMER_RIGHT_3_END_STABILITY=5.4000
8209
PRIMER_PAIR_3_COMPL_ANY=3.00
8210
PRIMER_PAIR_3_COMPL_END=1.00
8211
PRIMER_PRODUCT_SIZE_3=141
8212
PRIMER_PAIR_PENALTY_4=1.1760
8213
PRIMER_LEFT_4_PENALTY=1.095892
8214
PRIMER_RIGHT_4_PENALTY=0.080144
8215
PRIMER_LEFT_4_SEQUENCE=TCCCAACTCAGACAATCCTTG
8216
PRIMER_RIGHT_4_SEQUENCE=ATGATGCCTCTGACCTCCAC
8217
PRIMER_LEFT_4=54,21
8218
PRIMER_RIGHT_4=189,20
8219
PRIMER_LEFT_4_TM=60.096
8220
PRIMER_RIGHT_4_TM=60.080
8221
PRIMER_LEFT_4_GC_PERCENT=47.619
8222
PRIMER_RIGHT_4_GC_PERCENT=55.000
8223
PRIMER_LEFT_4_SELF_ANY=3.00
8224
PRIMER_RIGHT_4_SELF_ANY=4.00
8225
PRIMER_LEFT_4_SELF_END=3.00
8226
PRIMER_RIGHT_4_SELF_END=0.00
8227
PRIMER_LEFT_4_END_STABILITY=8.5000
8228
PRIMER_RIGHT_4_END_STABILITY=7.9000
8229
PRIMER_PAIR_4_COMPL_ANY=5.00
8230
PRIMER_PAIR_4_COMPL_END=1.00
8231
PRIMER_PRODUCT_SIZE_4=136
8232
=
8233
MARKER_NAME=33.RHAP64FC6.seq
8234
SEQUENCE=NNNNNNNNNNNNNNNNNCCAGAACAATGTCATCTGGACAGCTTGCAAAAATGTTAGCATCTGTTCTTGGTCTACCAATGCAAGTCTATGTGGTGAAGGTGTTTGTTATTTACAGTTTAGGAAATAAGGAATAAATCTTGAGATCATCGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACTGGAAGTTATTGGTAAAGNGCACAGAGGCTTTCTTTTCTTTTCTTTGTTAANTTCTTTTCAAAAAGGATAAAAAAATATCTTGTCAAACTTAAAACAGCTTATGAATTCTCCCCACTTGCTTCCCAACGTACTGTTTTCTCTGTAATTATACTGAATAACAAAGATTCTATATTTGTCNCATAGTTCTNGCTACTTAC
8235
INCLUDED_REGION=17,428
8236
TARGET=149,40,CA
8237
TARGET=190,54,GA
8238
PRIMER_COMMENT=CL is AAGGAATAAATCTTGAGATCATCG
8239
PRIMER_COMMENT=CR is AAAGAAAAGAAAGCCTCTGTGC
8240
PRIMER_PAIR_PENALTY=8.9046
8241
PRIMER_LEFT_PENALTY=6.104219
8242
PRIMER_RIGHT_PENALTY=2.800379
8243
PRIMER_LEFT_SEQUENCE=AAGGAATAAATCTTGAGATCATCG
8244
PRIMER_RIGHT_SEQUENCE=AAAGAAAAGAAAGCCTCTGTGC
8245
PRIMER_LEFT=124,24
8246
PRIMER_RIGHT=287,22
8247
PRIMER_LEFT_TM=57.896
8248
PRIMER_RIGHT_TM=59.200
8249
PRIMER_LEFT_GC_PERCENT=33.333
8250
PRIMER_RIGHT_GC_PERCENT=40.909
8251
PRIMER_LEFT_SELF_ANY=6.00
8252
PRIMER_RIGHT_SELF_ANY=3.00
8253
PRIMER_LEFT_SELF_END=3.00
8254
PRIMER_RIGHT_SELF_END=2.00
8255
PRIMER_LEFT_END_STABILITY=8.6000
8256
PRIMER_RIGHT_END_STABILITY=8.2000
8257
PRIMER_PAIR_COMPL_ANY=4.00
8258
PRIMER_PAIR_COMPL_END=1.00
8259
PRIMER_PRODUCT_SIZE=164
8260
PRIMER_PAIR_PENALTY_1=9.1373
8261
PRIMER_LEFT_1_PENALTY=6.336905
8262
PRIMER_RIGHT_1_PENALTY=2.800379
8263
PRIMER_LEFT_1_SEQUENCE=AAGGAATAAATCTTGAGATCATCGT
8264
PRIMER_RIGHT_1_SEQUENCE=AAAGAAAAGAAAGCCTCTGTGC
8265
PRIMER_LEFT_1=124,25
8266
PRIMER_RIGHT_1=287,22
8267
PRIMER_LEFT_1_TM=58.663
8268
PRIMER_RIGHT_1_TM=59.200
8269
PRIMER_LEFT_1_GC_PERCENT=32.000
8270
PRIMER_RIGHT_1_GC_PERCENT=40.909
8271
PRIMER_LEFT_1_SELF_ANY=6.00
8272
PRIMER_RIGHT_1_SELF_ANY=3.00
8273
PRIMER_LEFT_1_SELF_END=3.00
8274
PRIMER_RIGHT_1_SELF_END=2.00
8275
PRIMER_LEFT_1_END_STABILITY=8.0000
8276
PRIMER_RIGHT_1_END_STABILITY=8.2000
8277
PRIMER_PAIR_1_COMPL_ANY=4.00
8278
PRIMER_PAIR_1_COMPL_END=0.00
8279
PRIMER_PRODUCT_SIZE_1=164
8280
PRIMER_PAIR_PENALTY_2=9.2246
8281
PRIMER_LEFT_2_PENALTY=6.104219
8282
PRIMER_RIGHT_2_PENALTY=3.120339
8283
PRIMER_LEFT_2_SEQUENCE=AAGGAATAAATCTTGAGATCATCG
8284
PRIMER_RIGHT_2_SEQUENCE=AAGAAAAGAAAGCCTCTGTGC
8285
PRIMER_LEFT_2=124,24
8286
PRIMER_RIGHT_2=286,21
8287
PRIMER_LEFT_2_TM=57.896
8288
PRIMER_RIGHT_2_TM=57.880
8289
PRIMER_LEFT_2_GC_PERCENT=33.333
8290
PRIMER_RIGHT_2_GC_PERCENT=42.857
8291
PRIMER_LEFT_2_SELF_ANY=6.00
8292
PRIMER_RIGHT_2_SELF_ANY=3.00
8293
PRIMER_LEFT_2_SELF_END=3.00
8294
PRIMER_RIGHT_2_SELF_END=2.00
8295
PRIMER_LEFT_2_END_STABILITY=8.6000
8296
PRIMER_RIGHT_2_END_STABILITY=8.2000
8297
PRIMER_PAIR_2_COMPL_ANY=4.00
8298
PRIMER_PAIR_2_COMPL_END=1.00
8299
PRIMER_PRODUCT_SIZE_2=163
8300
PRIMER_PAIR_PENALTY_3=9.3827
8301
PRIMER_LEFT_3_PENALTY=6.582286
8302
PRIMER_RIGHT_3_PENALTY=2.800379
8303
PRIMER_LEFT_3_SEQUENCE=AGGAATAAATCTTGAGATCATCGT
8304
PRIMER_RIGHT_3_SEQUENCE=AAAGAAAAGAAAGCCTCTGTGC
8305
PRIMER_LEFT_3=125,24
8306
PRIMER_RIGHT_3=287,22
8307
PRIMER_LEFT_3_TM=57.418
8308
PRIMER_RIGHT_3_TM=59.200
8309
PRIMER_LEFT_3_GC_PERCENT=33.333
8310
PRIMER_RIGHT_3_GC_PERCENT=40.909
8311
PRIMER_LEFT_3_SELF_ANY=6.00
8312
PRIMER_RIGHT_3_SELF_ANY=3.00
8313
PRIMER_LEFT_3_SELF_END=3.00
8314
PRIMER_RIGHT_3_SELF_END=2.00
8315
PRIMER_LEFT_3_END_STABILITY=8.0000
8316
PRIMER_RIGHT_3_END_STABILITY=8.2000
8317
PRIMER_PAIR_3_COMPL_ANY=4.00
8318
PRIMER_PAIR_3_COMPL_END=0.00
8319
PRIMER_PRODUCT_SIZE_3=163
8320
PRIMER_PAIR_PENALTY_4=9.4572
8321
PRIMER_LEFT_4_PENALTY=6.336905
8322
PRIMER_RIGHT_4_PENALTY=3.120339
8323
PRIMER_LEFT_4_SEQUENCE=AAGGAATAAATCTTGAGATCATCGT
8324
PRIMER_RIGHT_4_SEQUENCE=AAGAAAAGAAAGCCTCTGTGC
8325
PRIMER_LEFT_4=124,25
8326
PRIMER_RIGHT_4=286,21
8327
PRIMER_LEFT_4_TM=58.663
8328
PRIMER_RIGHT_4_TM=57.880
8329
PRIMER_LEFT_4_GC_PERCENT=32.000
8330
PRIMER_RIGHT_4_GC_PERCENT=42.857
8331
PRIMER_LEFT_4_SELF_ANY=6.00
8332
PRIMER_RIGHT_4_SELF_ANY=3.00
8333
PRIMER_LEFT_4_SELF_END=3.00
8334
PRIMER_RIGHT_4_SELF_END=2.00
8335
PRIMER_LEFT_4_END_STABILITY=8.0000
8336
PRIMER_RIGHT_4_END_STABILITY=8.2000
8337
PRIMER_PAIR_4_COMPL_ANY=4.00
8338
PRIMER_PAIR_4_COMPL_END=0.00
8339
PRIMER_PRODUCT_SIZE_4=163
8340
=
8341
MARKER_NAME=34.RHAP64FD10.seq
8342
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNCACTAGGGAGCCTTTATCAAATAAGTAGCTTGACCAAATGCCTTTTCAGTACNNNNNNNNNNNNNNNGCCTTCTCTTTTCTGTATCGGACTATACTTCCCTGAGAAATTCAGAAGAAATTGAAAGGTGAACTACTTCACCATCTCCCCTCTGCGTGCATCCATCTGTTACAAAGGATATCGTTTGACATATATAACCATGTTAACCTTCACGATTTTAAGGAAGTCCTTGGTGGAGGACCACAAAGAATAGATTGACCTCAAAAGGAGGTCTATTGGTCTGAAAAGTTGGCACCCCCAAGTCCTGTGGCTGGGTACCCAAGGATACAAGAAAAATCACCTCGAAGGGTCTGAAAATCAGTTTTGTAGTTAGGAGGAAGAGCNTGGTAAACTGCTCTTCAAAACTACCTGATAGGGGAANATGAGTCCATTGTCTGGTTTGTTT
8343
INCLUDED_REGION=24,443
8344
TARGET=76,15,CT
8345
PRIMER_COMMENT=CL is GCTTGACCAAATGCCTTTTC
8346
PRIMER_COMMENT=CR is GTAACAGATGGATGCACGCA
8347
PRIMER_PAIR_PENALTY=1.0068
8348
PRIMER_LEFT_PENALTY=0.309288
8349
PRIMER_RIGHT_PENALTY=0.697466
8350
PRIMER_LEFT_SEQUENCE=GCTTGACCAAATGCCTTTTC
8351
PRIMER_RIGHT_SEQUENCE=TGTAACAGATGGATGCACGC
8352
PRIMER_LEFT=51,20
8353
PRIMER_RIGHT=194,20
8354
PRIMER_LEFT_TM=59.691
8355
PRIMER_RIGHT_TM=60.697
8356
PRIMER_LEFT_GC_PERCENT=45.000
8357
PRIMER_RIGHT_GC_PERCENT=50.000
8358
PRIMER_LEFT_SELF_ANY=6.00
8359
PRIMER_RIGHT_SELF_ANY=5.00
8360
PRIMER_LEFT_SELF_END=3.00
8361
PRIMER_RIGHT_SELF_END=2.00
8362
PRIMER_LEFT_END_STABILITY=7.3000
8363
PRIMER_RIGHT_END_STABILITY=9.9000
8364
PRIMER_PAIR_COMPL_ANY=3.00
8365
PRIMER_PAIR_COMPL_END=0.00
8366
PRIMER_PRODUCT_SIZE=144
8367
PRIMER_PAIR_PENALTY_1=1.0068
8368
PRIMER_LEFT_1_PENALTY=0.309288
8369
PRIMER_RIGHT_1_PENALTY=0.697466
8370
PRIMER_LEFT_1_SEQUENCE=GCTTGACCAAATGCCTTTTC
8371
PRIMER_RIGHT_1_SEQUENCE=GTAACAGATGGATGCACGCA
8372
PRIMER_LEFT_1=51,20
8373
PRIMER_RIGHT_1=193,20
8374
PRIMER_LEFT_1_TM=59.691
8375
PRIMER_RIGHT_1_TM=60.697
8376
PRIMER_LEFT_1_GC_PERCENT=45.000
8377
PRIMER_RIGHT_1_GC_PERCENT=50.000
8378
PRIMER_LEFT_1_SELF_ANY=6.00
8379
PRIMER_RIGHT_1_SELF_ANY=4.00
8380
PRIMER_LEFT_1_SELF_END=3.00
8381
PRIMER_RIGHT_1_SELF_END=4.00
8382
PRIMER_LEFT_1_END_STABILITY=7.3000
8383
PRIMER_RIGHT_1_END_STABILITY=9.9000
8384
PRIMER_PAIR_1_COMPL_ANY=3.00
8385
PRIMER_PAIR_1_COMPL_END=1.00
8386
PRIMER_PRODUCT_SIZE_1=143
8387
PRIMER_PAIR_PENALTY_2=1.1428
8388
PRIMER_LEFT_2_PENALTY=0.309288
8389
PRIMER_RIGHT_2_PENALTY=0.833530
8390
PRIMER_LEFT_2_SEQUENCE=GCTTGACCAAATGCCTTTTC
8391
PRIMER_RIGHT_2_SEQUENCE=TAACAGATGGATGCACGCAG
8392
PRIMER_LEFT_2=51,20
8393
PRIMER_RIGHT_2=192,20
8394
PRIMER_LEFT_2_TM=59.691
8395
PRIMER_RIGHT_2_TM=60.834
8396
PRIMER_LEFT_2_GC_PERCENT=45.000
8397
PRIMER_RIGHT_2_GC_PERCENT=50.000
8398
PRIMER_LEFT_2_SELF_ANY=6.00
8399
PRIMER_RIGHT_2_SELF_ANY=4.00
8400
PRIMER_LEFT_2_SELF_END=3.00
8401
PRIMER_RIGHT_2_SELF_END=2.00
8402
PRIMER_LEFT_2_END_STABILITY=7.3000
8403
PRIMER_RIGHT_2_END_STABILITY=10.2000
8404
PRIMER_PAIR_2_COMPL_ANY=3.00
8405
PRIMER_PAIR_2_COMPL_END=1.00
8406
PRIMER_PRODUCT_SIZE_2=142
8407
PRIMER_PAIR_PENALTY_3=1.2082
8408
PRIMER_LEFT_3_PENALTY=0.309288
8409
PRIMER_RIGHT_3_PENALTY=0.898913
8410
PRIMER_LEFT_3_SEQUENCE=GCTTGACCAAATGCCTTTTC
8411
PRIMER_RIGHT_3_SEQUENCE=CAGAGGGGAGATGGTGAAGT
8412
PRIMER_LEFT_3=51,20
8413
PRIMER_RIGHT_3=175,20
8414
PRIMER_LEFT_3_TM=59.691
8415
PRIMER_RIGHT_3_TM=59.101
8416
PRIMER_LEFT_3_GC_PERCENT=45.000
8417
PRIMER_RIGHT_3_GC_PERCENT=55.000
8418
PRIMER_LEFT_3_SELF_ANY=6.00
8419
PRIMER_RIGHT_3_SELF_ANY=2.00
8420
PRIMER_LEFT_3_SELF_END=3.00
8421
PRIMER_RIGHT_3_SELF_END=1.00
8422
PRIMER_LEFT_3_END_STABILITY=7.3000
8423
PRIMER_RIGHT_3_END_STABILITY=6.4000
8424
PRIMER_PAIR_3_COMPL_ANY=6.00
8425
PRIMER_PAIR_3_COMPL_END=4.00
8426
PRIMER_PRODUCT_SIZE_3=125
8427
PRIMER_PAIR_PENALTY_4=1.5105
8428
PRIMER_LEFT_4_PENALTY=0.309288
8429
PRIMER_RIGHT_4_PENALTY=1.201218
8430
PRIMER_LEFT_4_SEQUENCE=GCTTGACCAAATGCCTTTTC
8431
PRIMER_RIGHT_4_SEQUENCE=TAACAGATGGATGCACGCA
8432
PRIMER_LEFT_4=51,20
8433
PRIMER_RIGHT_4=192,19
8434
PRIMER_LEFT_4_TM=59.691
8435
PRIMER_RIGHT_4_TM=59.799
8436
PRIMER_LEFT_4_GC_PERCENT=45.000
8437
PRIMER_RIGHT_4_GC_PERCENT=47.368
8438
PRIMER_LEFT_4_SELF_ANY=6.00
8439
PRIMER_RIGHT_4_SELF_ANY=4.00
8440
PRIMER_LEFT_4_SELF_END=3.00
8441
PRIMER_RIGHT_4_SELF_END=4.00
8442
PRIMER_LEFT_4_END_STABILITY=7.3000
8443
PRIMER_RIGHT_4_END_STABILITY=9.9000
8444
PRIMER_PAIR_4_COMPL_ANY=3.00
8445
PRIMER_PAIR_4_COMPL_END=1.00
8446
PRIMER_PRODUCT_SIZE_4=142
8447
=
8448
MARKER_NAME=35.RHAP64FD11.seq
8449
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCCTCCTGAGAAATGCAGGTCAGGGTACCTTGGCAGGGAATCCCAAGGATCAGTACATGGCAGCTGAAAGTTGTCTTGGATGCAAGACTGGGAGTGAGGCTCAAGTCAGCCGAGCCTCAGCTCTGGATTCCTGAAACAGAATACCAGAATATGTTAGAGAGCGAGGAGACTAAACTAAACATCTGGGAGTCCTCAGCAGATTGTACAGATCCAGGGCTGAGGGNTCATAAGTATGTTGGTCTTTGTGGGGGTGGGAAAGGAACACTTATCTAGATCCCCACCTCCTTACTATGTGCTACTACGGGTGGNGGCAATGCCTTAAGGACCAAGGTTTTTTGTTTTGTTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGNGTGTGTGNGTTGCATNNAATGCANGGNGCAGGTGGTANTAATTGNNGGGGGNTAANNCANTGTTCTTCNTCTACCNNNTTCC
8450
INCLUDED_REGION=23,460
8451
TARGET=369,28,TG
8452
PRIMER_COMMENT=CL is
8453
PRIMER_COMMENT=CR is
8454
=
8455
MARKER_NAME=36.RHAP64FD6.seq
8456
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNCACATTTTTCTTTANGNNNNNNNANTGNAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCGCGCGCCCGNNAAGGCACAANTAACCAAGGANGGAGGTCAGACAACAAGTTTATGGCGTCAATTCNCTCTCACCTTTACATGGATNGCCNGNNNTACACTGCAAACATCCGGCACTGNACCGTTCTGCCAGTCCANGGTCTCCCNANNTTTGTCCCCCTCNCTGTCTCNCNGTCTCTGCCCTTGNTAGTCTGNCGNNNCTCCGATANCCTCNNNACCCCNGGNNTTCTGCTCTCNGTGACTTCCCCCTCNCCCTGNCTCCCNCNGTCNGNTCCGTCTNGTCCNTGCNNTTNCCTAGTTTCNCNCCNNTNNGTNTCCCNGGNNTGGCGNGGTCNGNNNGTGCCNCCNNNTATNGTGA
8457
INCLUDED_REGION=27,434
8458
TARGET=57,46,GT
8459
PRIMER_COMMENT=CL is
8460
PRIMER_COMMENT=CR is
8461
=
8462
MARKER_NAME=37.RHAP64FE4.seq
8463
SEQUENCE=NNNNNNNNNNNNNNNNNNNNCCAGCCTTTGATACAGGAACAGGAGGACCCAGTCCGTAAAACCAAAGGTGAGGGAGAAACTAAGACATTCTCAGATAAAGAAACTAAGGAAATGTCTTGATAATGTAACAAGCCTTGGAAACAGCTTTGGCTACAAAGCACCGCTTCCTCTCATGGAACCCACAGCAATCGTTGGCAAGGGCAGTGAGACAGACGGCTCAGCAGTTCTAAGTACTCGGTGACCTGAATTTAACCCACATGGAAGACGGCAACAATCAACCCTTGAAAGTCTTCTGAGTGTGTACCCTGGCACCAAAGGACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATTAGGTTTTTCTTTTTTCTTTTTCTTTTTTTAAAGTAGAAGCAGGCAGCCGGAAGTGGTANCTCACAATCTTTATCTTTT
8464
INCLUDED_REGION=20,428
8465
TARGET=322,44,CA
8466
PRIMER_COMMENT=CL is TCTGAGTGTGTACCCTGGCA
8467
PRIMER_COMMENT=CR is CGGCTGCCTGCTTCTACTT
8468
PRIMER_PAIR_PENALTY=1.9930
8469
PRIMER_LEFT_PENALTY=0.309402
8470
PRIMER_RIGHT_PENALTY=1.683594
8471
PRIMER_LEFT_SEQUENCE=TCTGAGTGTGTACCCTGGCA
8472
PRIMER_RIGHT_SEQUENCE=CGGCTGCCTGCTTCTACTT
8473
PRIMER_LEFT=291,20
8474
PRIMER_RIGHT=418,19
8475
PRIMER_LEFT_TM=60.309
8476
PRIMER_RIGHT_TM=60.684
8477
PRIMER_LEFT_GC_PERCENT=55.000
8478
PRIMER_RIGHT_GC_PERCENT=57.895
8479
PRIMER_LEFT_SELF_ANY=4.00
8480
PRIMER_RIGHT_SELF_ANY=5.00
8481
PRIMER_LEFT_SELF_END=3.00
8482
PRIMER_RIGHT_SELF_END=0.00
8483
PRIMER_LEFT_END_STABILITY=10.0000
8484
PRIMER_RIGHT_END_STABILITY=5.7000
8485
PRIMER_PAIR_COMPL_ANY=4.00
8486
PRIMER_PAIR_COMPL_END=1.00
8487
PRIMER_PRODUCT_SIZE=128
8488
PRIMER_PAIR_PENALTY_1=2.0674
8489
PRIMER_LEFT_1_PENALTY=0.383798
8490
PRIMER_RIGHT_1_PENALTY=1.683594
8491
PRIMER_LEFT_1_SEQUENCE=ACATGGAAGACGGCAACAAT
8492
PRIMER_RIGHT_1_SEQUENCE=CGGCTGCCTGCTTCTACTT
8493
PRIMER_LEFT_1=255,20
8494
PRIMER_RIGHT_1=418,19
8495
PRIMER_LEFT_1_TM=60.384
8496
PRIMER_RIGHT_1_TM=60.684
8497
PRIMER_LEFT_1_GC_PERCENT=45.000
8498
PRIMER_RIGHT_1_GC_PERCENT=57.895
8499
PRIMER_LEFT_1_SELF_ANY=4.00
8500
PRIMER_RIGHT_1_SELF_ANY=5.00
8501
PRIMER_LEFT_1_SELF_END=2.00
8502
PRIMER_RIGHT_1_SELF_END=0.00
8503
PRIMER_LEFT_1_END_STABILITY=6.6000
8504
PRIMER_RIGHT_1_END_STABILITY=5.7000
8505
PRIMER_PAIR_1_COMPL_ANY=6.00
8506
PRIMER_PAIR_1_COMPL_END=3.00
8507
PRIMER_PRODUCT_SIZE_1=164
8508
PRIMER_PAIR_PENALTY_2=2.1879
8509
PRIMER_LEFT_2_PENALTY=0.504346
8510
PRIMER_RIGHT_2_PENALTY=1.683594
8511
PRIMER_LEFT_2_SEQUENCE=GGAAGACGGCAACAATCAAC
8512
PRIMER_RIGHT_2_SEQUENCE=CGGCTGCCTGCTTCTACTT
8513
PRIMER_LEFT_2=259,20
8514
PRIMER_RIGHT_2=418,19
8515
PRIMER_LEFT_2_TM=60.504
8516
PRIMER_RIGHT_2_TM=60.684
8517
PRIMER_LEFT_2_GC_PERCENT=50.000
8518
PRIMER_RIGHT_2_GC_PERCENT=57.895
8519
PRIMER_LEFT_2_SELF_ANY=2.00
8520
PRIMER_RIGHT_2_SELF_ANY=5.00
8521
PRIMER_LEFT_2_SELF_END=0.00
8522
PRIMER_RIGHT_2_SELF_END=0.00
8523
PRIMER_LEFT_2_END_STABILITY=6.7000
8524
PRIMER_RIGHT_2_END_STABILITY=5.7000
8525
PRIMER_PAIR_2_COMPL_ANY=6.00
8526
PRIMER_PAIR_2_COMPL_END=1.00
8527
PRIMER_PRODUCT_SIZE_2=160
8528
PRIMER_PAIR_PENALTY_3=2.1879
8529
PRIMER_LEFT_3_PENALTY=0.504346
8530
PRIMER_RIGHT_3_PENALTY=1.683594
8531
PRIMER_LEFT_3_SEQUENCE=GAAGACGGCAACAATCAACC
8532
PRIMER_RIGHT_3_SEQUENCE=CGGCTGCCTGCTTCTACTT
8533
PRIMER_LEFT_3=260,20
8534
PRIMER_RIGHT_3=418,19
8535
PRIMER_LEFT_3_TM=60.504
8536
PRIMER_RIGHT_3_TM=60.684
8537
PRIMER_LEFT_3_GC_PERCENT=50.000
8538
PRIMER_RIGHT_3_GC_PERCENT=57.895
8539
PRIMER_LEFT_3_SELF_ANY=2.00
8540
PRIMER_RIGHT_3_SELF_ANY=5.00
8541
PRIMER_LEFT_3_SELF_END=0.00
8542
PRIMER_RIGHT_3_SELF_END=0.00
8543
PRIMER_LEFT_3_END_STABILITY=8.2000
8544
PRIMER_RIGHT_3_END_STABILITY=5.7000
8545
PRIMER_PAIR_3_COMPL_ANY=6.00
8546
PRIMER_PAIR_3_COMPL_END=2.00
8547
PRIMER_PRODUCT_SIZE_3=159
8548
PRIMER_PAIR_PENALTY_4=2.2380
8549
PRIMER_LEFT_4_PENALTY=0.309402
8550
PRIMER_RIGHT_4_PENALTY=1.928601
8551
PRIMER_LEFT_4_SEQUENCE=TCTGAGTGTGTACCCTGGCA
8552
PRIMER_RIGHT_4_SEQUENCE=CGGCTGCCTGCTTCTACTTT
8553
PRIMER_LEFT_4=291,20
8554
PRIMER_RIGHT_4=418,20
8555
PRIMER_LEFT_4_TM=60.309
8556
PRIMER_RIGHT_4_TM=61.929
8557
PRIMER_LEFT_4_GC_PERCENT=55.000
8558
PRIMER_RIGHT_4_GC_PERCENT=55.000
8559
PRIMER_LEFT_4_SELF_ANY=4.00
8560
PRIMER_RIGHT_4_SELF_ANY=5.00
8561
PRIMER_LEFT_4_SELF_END=3.00
8562
PRIMER_RIGHT_4_SELF_END=0.00
8563
PRIMER_LEFT_4_END_STABILITY=10.0000
8564
PRIMER_RIGHT_4_END_STABILITY=6.7000
8565
PRIMER_PAIR_4_COMPL_ANY=4.00
8566
PRIMER_PAIR_4_COMPL_END=2.00
8567
PRIMER_PRODUCT_SIZE_4=128
8568
=
8569
MARKER_NAME=38.RHAP64FE5.seq
8570
SEQUENCE=NNNNNNNNNNNNNNNNNCATGTAGAATGTGTTTGTTTGTTTTTGTAAACTTAACTTAATCAAGGATATTCCTCACAGGCATACTCAGAAGATAATCTACAAAATCCCTCACATGATGTAGACANGTGGTCTACATCAGTAGGTCTCCACACTCTAGTGGCTGTTTTCTCCAGGTAGTGCTTAACTCTGGCTTTTAGCTGTTTCTAAATATTTCCTGGGAAAATTAAGGTTAACGAGAAACATATTGACCTTCGNTGTTCTTCCTGTGTGGATAATCTGACTTTGGACAGACCTAGGTTGTGAGGAATGCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCGTGCGCGTGCCAGAGGTCGACCACAGCACAGGTGTTCTTTCTCAGAAGCAATCTACCNTATTTTATTTTATNC
8571
INCLUDED_REGION=17,424
8572
TARGET=310,56,GT
8573
PRIMER_COMMENT=CL is CCTAGGTTGTGAGGAATGCC
8574
PRIMER_COMMENT=CR is TGCTTCTGAGAAAGAACACCTG
8575
PRIMER_PAIR_PENALTY=2.7935
8576
PRIMER_LEFT_PENALTY=0.449829
8577
PRIMER_RIGHT_PENALTY=2.343680
8578
PRIMER_LEFT_SEQUENCE=CCTAGGTTGTGAGGAATGCC
8579
PRIMER_RIGHT_SEQUENCE=TGCTTCTGAGAAAGAACACCTG
8580
PRIMER_LEFT=290,20
8581
PRIMER_RIGHT=417,22
8582
PRIMER_LEFT_TM=59.550
8583
PRIMER_RIGHT_TM=59.656
8584
PRIMER_LEFT_GC_PERCENT=55.000
8585
PRIMER_RIGHT_GC_PERCENT=45.455
8586
PRIMER_LEFT_SELF_ANY=6.00
8587
PRIMER_RIGHT_SELF_ANY=7.00
8588
PRIMER_LEFT_SELF_END=4.00
8589
PRIMER_RIGHT_SELF_END=2.00
8590
PRIMER_LEFT_END_STABILITY=9.6000
8591
PRIMER_RIGHT_END_STABILITY=7.9000
8592
PRIMER_PAIR_COMPL_ANY=4.00
8593
PRIMER_PAIR_COMPL_END=1.00
8594
PRIMER_PRODUCT_SIZE=128
8595
PRIMER_PAIR_PENALTY_1=2.9299
8596
PRIMER_LEFT_1_PENALTY=0.449829
8597
PRIMER_RIGHT_1_PENALTY=2.480050
8598
PRIMER_LEFT_1_SEQUENCE=CCTAGGTTGTGAGGAATGCC
8599
PRIMER_RIGHT_1_SEQUENCE=TTCTGAGAAAGAACACCTGTGC
8600
PRIMER_LEFT_1=290,20
8601
PRIMER_RIGHT_1=414,22
8602
PRIMER_LEFT_1_TM=59.550
8603
PRIMER_RIGHT_1_TM=59.520
8604
PRIMER_LEFT_1_GC_PERCENT=55.000
8605
PRIMER_RIGHT_1_GC_PERCENT=45.455
8606
PRIMER_LEFT_1_SELF_ANY=6.00
8607
PRIMER_RIGHT_1_SELF_ANY=7.00
8608
PRIMER_LEFT_1_SELF_END=4.00
8609
PRIMER_RIGHT_1_SELF_END=4.00
8610
PRIMER_LEFT_1_END_STABILITY=9.6000
8611
PRIMER_RIGHT_1_END_STABILITY=8.2000
8612
PRIMER_PAIR_1_COMPL_ANY=4.00
8613
PRIMER_PAIR_1_COMPL_END=1.00
8614
PRIMER_PRODUCT_SIZE_1=125
8615
PRIMER_PAIR_PENALTY_2=3.5661
8616
PRIMER_LEFT_2_PENALTY=2.343332
8617
PRIMER_RIGHT_2_PENALTY=1.222816
8618
PRIMER_LEFT_2_SEQUENCE=GGACAGACCTAGGTTGTGAGG
8619
PRIMER_RIGHT_2_SEQUENCE=AAAGAACACCTGTGCTGTGG
8620
PRIMER_LEFT_2=283,21
8621
PRIMER_RIGHT_2=407,20
8622
PRIMER_LEFT_2_TM=58.657
8623
PRIMER_RIGHT_2_TM=58.777
8624
PRIMER_LEFT_2_GC_PERCENT=57.143
8625
PRIMER_RIGHT_2_GC_PERCENT=50.000
8626
PRIMER_LEFT_2_SELF_ANY=10.00
8627
PRIMER_RIGHT_2_SELF_ANY=6.00
8628
PRIMER_LEFT_2_SELF_END=3.00
8629
PRIMER_RIGHT_2_SELF_END=2.00
8630
PRIMER_LEFT_2_END_STABILITY=8.2000
8631
PRIMER_RIGHT_2_END_STABILITY=8.2000
8632
PRIMER_PAIR_2_COMPL_ANY=5.00
8633
PRIMER_PAIR_2_COMPL_END=0.00
8634
PRIMER_PRODUCT_SIZE_2=125
8635
PRIMER_PAIR_PENALTY_3=3.5793
8636
PRIMER_LEFT_3_PENALTY=2.366796
8637
PRIMER_RIGHT_3_PENALTY=1.212518
8638
PRIMER_LEFT_3_SEQUENCE=TTTGGACAGACCTAGGTTGTGA
8639
PRIMER_RIGHT_3_SEQUENCE=AAGAACACCTGTGCTGTGGTC
8640
PRIMER_LEFT_3=280,22
8641
PRIMER_RIGHT_3=406,21
8642
PRIMER_LEFT_3_TM=59.633
8643
PRIMER_RIGHT_3_TM=60.213
8644
PRIMER_LEFT_3_GC_PERCENT=45.455
8645
PRIMER_RIGHT_3_GC_PERCENT=52.381
8646
PRIMER_LEFT_3_SELF_ANY=10.00
8647
PRIMER_RIGHT_3_SELF_ANY=6.00
8648
PRIMER_LEFT_3_SELF_END=6.00
8649
PRIMER_RIGHT_3_SELF_END=2.00
8650
PRIMER_LEFT_3_END_STABILITY=6.7000
8651
PRIMER_RIGHT_3_END_STABILITY=7.9000
8652
PRIMER_PAIR_3_COMPL_ANY=5.00
8653
PRIMER_PAIR_3_COMPL_END=2.00
8654
PRIMER_PRODUCT_SIZE_3=127
8655
PRIMER_PAIR_PENALTY_4=3.5896
8656
PRIMER_LEFT_4_PENALTY=2.366796
8657
PRIMER_RIGHT_4_PENALTY=1.222816
8658
PRIMER_LEFT_4_SEQUENCE=TTTGGACAGACCTAGGTTGTGA
8659
PRIMER_RIGHT_4_SEQUENCE=AAAGAACACCTGTGCTGTGG
8660
PRIMER_LEFT_4=280,22
8661
PRIMER_RIGHT_4=407,20
8662
PRIMER_LEFT_4_TM=59.633
8663
PRIMER_RIGHT_4_TM=58.777
8664
PRIMER_LEFT_4_GC_PERCENT=45.455
8665
PRIMER_RIGHT_4_GC_PERCENT=50.000
8666
PRIMER_LEFT_4_SELF_ANY=10.00
8667
PRIMER_RIGHT_4_SELF_ANY=6.00
8668
PRIMER_LEFT_4_SELF_END=6.00
8669
PRIMER_RIGHT_4_SELF_END=2.00
8670
PRIMER_LEFT_4_END_STABILITY=6.7000
8671
PRIMER_RIGHT_4_END_STABILITY=8.2000
8672
PRIMER_PAIR_4_COMPL_ANY=5.00
8673
PRIMER_PAIR_4_COMPL_END=1.00
8674
PRIMER_PRODUCT_SIZE_4=128
8675
=
8676
MARKER_NAME=39.RHAP64FE12.seq
8677
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNACCCCCCANAGCGTCCGTCTCNGNNNNNNNNNNNNNNNNNNNNNNNATGNANNNNNNNNNNNNNNNNNNTANGCCNGNNNNNNNNNNNNNNNNNNNNNNNATATGTCNGTGTGCCNCNNNNNNNNNNNNNNNNNNTATATCNGTGTATATGTGNGTGTACCCNNATGTGTGNGTACNCCTGTCNGTGNATGTGTGTATGNCTCNGCACCNCNCNNNCNCNCTNNATGNCNNNATANNTANCTCNANGTGNNCGCCCCNATGNACATNNCNGTGCCNACANNTCNGNATNCGNCCCNCNATCTCNANCNANGTCNGNNNNNANGNNNGCATATCNNNGTANNCNNANNTCNNTCNATACCNATCTANNNGNNNNNANCNANNNNNCNNNNNATANCCNCNCNCNGTCNCCNATNTANACANNNANNNNTNNGT
8678
INCLUDED_REGION=23,432
8679
TARGET=47,22,GT
8680
TARGET=74,18,TG
8681
TARGET=101,22,GT
8682
TARGET=140,18,TG
8683
PRIMER_COMMENT=CL is
8684
PRIMER_COMMENT=CR is
8685
=
8686
MARKER_NAME=39.RHAP64FE6.seq
8687
SEQUENCE=NNNNNNNNNNNNNNNNCCTTTACTTGAATGAAAAGTATTGTTTCTGTCTGTCTCTGTCTCTTTGTCTTTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGGGNAGGGNGCGTTACCCACCATAGATTATCCCCGACAATTTCAGTCTTCTCTGAGAATATCATAGTANAGACCCACCAAAATTGTCTCATTTNCAACACTGATCTTGCAAAGAAAATTAACATAAATCCGNNAAACTAATACTGAATANTTATAATATATCCATATCATGTCAACTATTTACATTCATAGAGTCCTCATCTAGAAAAACAATANTCTTTGTTTNTACAGTGGTTTGTACATGGC
8688
INCLUDED_REGION=16,425
8689
TARGET=70,36,TC
8690
TARGET=107,54,CT
8691
TARGET=162,32,TG
8692
PRIMER_COMMENT=CL is TTCTGTCTGTCTCTGTCTCTTTGTC
8693
PRIMER_COMMENT=CR is TCGGGGATAATCTATGGTGG
8694
PRIMER_PAIR_PENALTY=6.1626
8695
PRIMER_LEFT_PENALTY=5.761718
8696
PRIMER_RIGHT_PENALTY=0.400928
8697
PRIMER_LEFT_SEQUENCE=TTCTGTCTGTCTCTGTCTCTTTGTC
8698
PRIMER_RIGHT_SEQUENCE=TCGGGGATAATCTATGGTGG
8699
PRIMER_LEFT=41,25
8700
PRIMER_RIGHT=231,20
8701
PRIMER_LEFT_TM=59.238
8702
PRIMER_RIGHT_TM=59.599
8703
PRIMER_LEFT_GC_PERCENT=44.000
8704
PRIMER_RIGHT_GC_PERCENT=50.000
8705
PRIMER_LEFT_SELF_ANY=1.00
8706
PRIMER_RIGHT_SELF_ANY=5.00
8707
PRIMER_LEFT_SELF_END=1.00
8708
PRIMER_RIGHT_SELF_END=0.00
8709
PRIMER_LEFT_END_STABILITY=6.7000
8710
PRIMER_RIGHT_END_STABILITY=9.4000
8711
PRIMER_PAIR_COMPL_ANY=3.00
8712
PRIMER_PAIR_COMPL_END=1.00
8713
PRIMER_PRODUCT_SIZE=191
8714
PRIMER_PAIR_PENALTY_1=6.2354
8715
PRIMER_LEFT_1_PENALTY=5.761718
8716
PRIMER_RIGHT_1_PENALTY=0.473695
8717
PRIMER_LEFT_1_SEQUENCE=TTCTGTCTGTCTCTGTCTCTTTGTC
8718
PRIMER_RIGHT_1_SEQUENCE=GAAGACTGAAATTGTCGGGG
8719
PRIMER_LEFT_1=41,25
8720
PRIMER_RIGHT_1=245,20
8721
PRIMER_LEFT_1_TM=59.238
8722
PRIMER_RIGHT_1_TM=59.526
8723
PRIMER_LEFT_1_GC_PERCENT=44.000
8724
PRIMER_RIGHT_1_GC_PERCENT=50.000
8725
PRIMER_LEFT_1_SELF_ANY=1.00
8726
PRIMER_RIGHT_1_SELF_ANY=4.00
8727
PRIMER_LEFT_1_SELF_END=1.00
8728
PRIMER_RIGHT_1_SELF_END=0.00
8729
PRIMER_LEFT_1_END_STABILITY=6.7000
8730
PRIMER_RIGHT_1_END_STABILITY=12.9000
8731
PRIMER_PAIR_1_COMPL_ANY=6.00
8732
PRIMER_PAIR_1_COMPL_END=1.00
8733
PRIMER_PRODUCT_SIZE_1=205
8734
PRIMER_PAIR_PENALTY_2=6.2354
8735
PRIMER_LEFT_2_PENALTY=5.761718
8736
PRIMER_RIGHT_2_PENALTY=0.473695
8737
PRIMER_LEFT_2_SEQUENCE=TTCTGTCTGTCTCTGTCTCTTTGTC
8738
PRIMER_RIGHT_2_SEQUENCE=AAGACTGAAATTGTCGGGGA
8739
PRIMER_LEFT_2=41,25
8740
PRIMER_RIGHT_2=244,20
8741
PRIMER_LEFT_2_TM=59.238
8742
PRIMER_RIGHT_2_TM=59.526
8743
PRIMER_LEFT_2_GC_PERCENT=44.000
8744
PRIMER_RIGHT_2_GC_PERCENT=45.000
8745
PRIMER_LEFT_2_SELF_ANY=1.00
8746
PRIMER_RIGHT_2_SELF_ANY=4.00
8747
PRIMER_LEFT_2_SELF_END=1.00
8748
PRIMER_RIGHT_2_SELF_END=1.00
8749
PRIMER_LEFT_2_END_STABILITY=6.7000
8750
PRIMER_RIGHT_2_END_STABILITY=10.9000
8751
PRIMER_PAIR_2_COMPL_ANY=6.00
8752
PRIMER_PAIR_2_COMPL_END=2.00
8753
PRIMER_PRODUCT_SIZE_2=204
8754
PRIMER_PAIR_PENALTY_3=6.4736
8755
PRIMER_LEFT_3_PENALTY=6.072692
8756
PRIMER_RIGHT_3_PENALTY=0.400928
8757
PRIMER_LEFT_3_SEQUENCE=TTTCTGTCTGTCTCTGTCTCTTTG
8758
PRIMER_RIGHT_3_SEQUENCE=TCGGGGATAATCTATGGTGG
8759
PRIMER_LEFT_3=40,24
8760
PRIMER_RIGHT_3=231,20
8761
PRIMER_LEFT_3_TM=57.927
8762
PRIMER_RIGHT_3_TM=59.599
8763
PRIMER_LEFT_3_GC_PERCENT=41.667
8764
PRIMER_RIGHT_3_GC_PERCENT=50.000
8765
PRIMER_LEFT_3_SELF_ANY=1.00
8766
PRIMER_RIGHT_3_SELF_ANY=5.00
8767
PRIMER_LEFT_3_SELF_END=0.00
8768
PRIMER_RIGHT_3_SELF_END=0.00
8769
PRIMER_LEFT_3_END_STABILITY=7.3000
8770
PRIMER_RIGHT_3_END_STABILITY=9.4000
8771
PRIMER_PAIR_3_COMPL_ANY=2.00
8772
PRIMER_PAIR_3_COMPL_END=0.00
8773
PRIMER_PRODUCT_SIZE_3=192
8774
PRIMER_PAIR_PENALTY_4=6.4736
8775
PRIMER_LEFT_4_PENALTY=6.072692
8776
PRIMER_RIGHT_4_PENALTY=0.400928
8777
PRIMER_LEFT_4_SEQUENCE=TCTGTCTCTGTCTCTTTGTCTTTG
8778
PRIMER_RIGHT_4_SEQUENCE=TCGGGGATAATCTATGGTGG
8779
PRIMER_LEFT_4=46,24
8780
PRIMER_RIGHT_4=231,20
8781
PRIMER_LEFT_4_TM=57.927
8782
PRIMER_RIGHT_4_TM=59.599
8783
PRIMER_LEFT_4_GC_PERCENT=41.667
8784
PRIMER_RIGHT_4_GC_PERCENT=50.000
8785
PRIMER_LEFT_4_SELF_ANY=1.00
8786
PRIMER_RIGHT_4_SELF_ANY=5.00
8787
PRIMER_LEFT_4_SELF_END=0.00
8788
PRIMER_RIGHT_4_SELF_END=0.00
8789
PRIMER_LEFT_4_END_STABILITY=7.3000
8790
PRIMER_RIGHT_4_END_STABILITY=9.4000
8791
PRIMER_PAIR_4_COMPL_ANY=3.00
8792
PRIMER_PAIR_4_COMPL_END=0.00
8793
PRIMER_PRODUCT_SIZE_4=186
8794
=
8795
MARKER_NAME=41.RHAP64FF11.seq
8796
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCATGATATGGGCGTGGTTTGTGTGCTTGCTAAGTCAGTGAAGCACTCTCTTGAAGGAAGGAGGACCTGAATACAATCCCCCAGAATCTATGATGAGTTGGAAGCAGAACTGTCCATAGTTTTGCAAGACTGTATTTCTTGTGCTGGTATGATGGAGTCACATGATCCATTCAATCATTTGCCAGCCAGACTAACCTCACTAGTTAAGTTTAAGGCTGATAAGGGACCAAATATTAAAAAGAAAGTTGATTTCCAGGAGGAACATCTGAGGTTGTCTTTTAGCCAGTACACATTTGTTTGCAAATATGCACACATACTAGAGAACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGGGGGAATGGGGGGAGTTCAGAAAATCTCCAGGGATCNGTTNTNA
8797
INCLUDED_REGION=23,446
8798
TARGET=348,74,AC
8799
PRIMER_COMMENT=CL is TCCAGGAGGAACATCTGAGG
8800
PRIMER_COMMENT=CR is TTCTGAACTCCCCCCATTC
8801
PRIMER_PAIR_PENALTY=1.3440
8802
PRIMER_LEFT_PENALTY=0.190960
8803
PRIMER_RIGHT_PENALTY=1.152999
8804
PRIMER_LEFT_SEQUENCE=TCCAGGAGGAACATCTGAGG
8805
PRIMER_RIGHT_SEQUENCE=TTCTGAACTCCCCCCATTC
8806
PRIMER_LEFT=273,20
8807
PRIMER_RIGHT=446,19
8808
PRIMER_LEFT_TM=60.191
8809
PRIMER_RIGHT_TM=59.847
8810
PRIMER_LEFT_GC_PERCENT=55.000
8811
PRIMER_RIGHT_GC_PERCENT=52.632
8812
PRIMER_LEFT_SELF_ANY=5.00
8813
PRIMER_RIGHT_SELF_ANY=5.00
8814
PRIMER_LEFT_SELF_END=3.00
8815
PRIMER_RIGHT_SELF_END=0.00
8816
PRIMER_LEFT_END_STABILITY=8.2000
8817
PRIMER_RIGHT_END_STABILITY=6.9000
8818
PRIMER_PAIR_COMPL_ANY=4.00
8819
PRIMER_PAIR_COMPL_END=1.00
8820
PRIMER_PRODUCT_SIZE=174
8821
PRIMER_PAIR_PENALTY_1=1.3827
8822
PRIMER_LEFT_1_PENALTY=0.190960
8823
PRIMER_RIGHT_1_PENALTY=1.191728
8824
PRIMER_LEFT_1_SEQUENCE=TCCAGGAGGAACATCTGAGG
8825
PRIMER_RIGHT_1_SEQUENCE=TTTCTGAACTCCCCCCATTC
8826
PRIMER_LEFT_1=273,20
8827
PRIMER_RIGHT_1=447,20
8828
PRIMER_LEFT_1_TM=60.191
8829
PRIMER_RIGHT_1_TM=61.192
8830
PRIMER_LEFT_1_GC_PERCENT=55.000
8831
PRIMER_RIGHT_1_GC_PERCENT=50.000
8832
PRIMER_LEFT_1_SELF_ANY=5.00
8833
PRIMER_RIGHT_1_SELF_ANY=5.00
8834
PRIMER_LEFT_1_SELF_END=3.00
8835
PRIMER_RIGHT_1_SELF_END=0.00
8836
PRIMER_LEFT_1_END_STABILITY=8.2000
8837
PRIMER_RIGHT_1_END_STABILITY=6.9000
8838
PRIMER_PAIR_1_COMPL_ANY=4.00
8839
PRIMER_PAIR_1_COMPL_END=1.00
8840
PRIMER_PRODUCT_SIZE_1=175
8841
PRIMER_PAIR_PENALTY_2=1.5621
8842
PRIMER_LEFT_2_PENALTY=0.898913
8843
PRIMER_RIGHT_2_PENALTY=0.663161
8844
PRIMER_LEFT_2_SEQUENCE=CCAGGAGGAACATCTGAGGT
8845
PRIMER_RIGHT_2_SEQUENCE=TTTTCTGAACTCCCCCCATT
8846
PRIMER_LEFT_2=274,20
8847
PRIMER_RIGHT_2=448,20
8848
PRIMER_LEFT_2_TM=59.101
8849
PRIMER_RIGHT_2_TM=60.663
8850
PRIMER_LEFT_2_GC_PERCENT=55.000
8851
PRIMER_RIGHT_2_GC_PERCENT=45.000
8852
PRIMER_LEFT_2_SELF_ANY=5.00
8853
PRIMER_RIGHT_2_SELF_ANY=5.00
8854
PRIMER_LEFT_2_SELF_END=5.00
8855
PRIMER_RIGHT_2_SELF_END=1.00
8856
PRIMER_LEFT_2_END_STABILITY=7.6000
8857
PRIMER_RIGHT_2_END_STABILITY=8.4000
8858
PRIMER_PAIR_2_COMPL_ANY=4.00
8859
PRIMER_PAIR_2_COMPL_END=0.00
8860
PRIMER_PRODUCT_SIZE_2=175
8861
PRIMER_PAIR_PENALTY_3=1.6652
8862
PRIMER_LEFT_3_PENALTY=1.293870
8863
PRIMER_RIGHT_3_PENALTY=0.371364
8864
PRIMER_LEFT_3_SEQUENCE=CAGGAGGAACATCTGAGGTTG
8865
PRIMER_RIGHT_3_SEQUENCE=ATTTTCTGAACTCCCCCCAT
8866
PRIMER_LEFT_3=275,21
8867
PRIMER_RIGHT_3=449,20
8868
PRIMER_LEFT_3_TM=59.706
8869
PRIMER_RIGHT_3_TM=59.629
8870
PRIMER_LEFT_3_GC_PERCENT=52.381
8871
PRIMER_RIGHT_3_GC_PERCENT=45.000
8872
PRIMER_LEFT_3_SELF_ANY=7.00
8873
PRIMER_RIGHT_3_SELF_ANY=5.00
8874
PRIMER_LEFT_3_SELF_END=5.00
8875
PRIMER_RIGHT_3_SELF_END=2.00
8876
PRIMER_LEFT_3_END_STABILITY=8.2000
8877
PRIMER_RIGHT_3_END_STABILITY=9.6000
8878
PRIMER_PAIR_3_COMPL_ANY=4.00
8879
PRIMER_PAIR_3_COMPL_END=1.00
8880
PRIMER_PRODUCT_SIZE_3=175
8881
PRIMER_PAIR_PENALTY_4=1.7244
8882
PRIMER_LEFT_4_PENALTY=1.353069
8883
PRIMER_RIGHT_4_PENALTY=0.371364
8884
PRIMER_LEFT_4_SEQUENCE=GCCAGTACACATTTGTTTGCA
8885
PRIMER_RIGHT_4_SEQUENCE=ATTTTCTGAACTCCCCCCAT
8886
PRIMER_LEFT_4=303,21
8887
PRIMER_RIGHT_4=449,20
8888
PRIMER_LEFT_4_TM=59.647
8889
PRIMER_RIGHT_4_TM=59.629
8890
PRIMER_LEFT_4_GC_PERCENT=42.857
8891
PRIMER_RIGHT_4_GC_PERCENT=45.000
8892
PRIMER_LEFT_4_SELF_ANY=6.00
8893
PRIMER_RIGHT_4_SELF_ANY=5.00
8894
PRIMER_LEFT_4_SELF_END=4.00
8895
PRIMER_RIGHT_4_SELF_END=2.00
8896
PRIMER_LEFT_4_END_STABILITY=8.8000
8897
PRIMER_RIGHT_4_END_STABILITY=9.6000
8898
PRIMER_PAIR_4_COMPL_ANY=4.00
8899
PRIMER_PAIR_4_COMPL_END=1.00
8900
PRIMER_PRODUCT_SIZE_4=147
8901
=
8902
MARKER_NAME=44.RHAP64FG5.seq
8903
SEQUENCE=NNNNNNNNNNNNNNNNNCCTCTTTCCTCTGTAGAAAATGCACTGCTAATAACTTTGCTCAAATATATCTCACAACATTTCTTAATATGGCTCCAGGTAGCAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNACAGACAGACAGACAGACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGCAGAAAGAGTGAAACCTAGGTACCCTGGTATTATAAAGAACACATTTTCAGCTCAAAAGCGAGCAGGGNGTCGCTCCACTCTCCATGGGTCTGGGTGTGGCTCCNTCNCCACACTGAAGGGTCTGNTGAGCTCCTGNAGGGGTAACAGGGGCTGCNTCCTCANATTANGGGTTCAGAGGNTGGNGGGTGNGCCACATTTTTTCAAANACA
8904
INCLUDED_REGION=17,435
8905
TARGET=103,40,CA
8906
TARGET=144,22,AG
8907
TARGET=186,54,AC
8908
PRIMER_COMMENT=CL is TCCTCTGTAGAAAATGCACTGC
8909
PRIMER_COMMENT=CR is CAGACCCATGGAGAGTGGAG
8910
PRIMER_PAIR_PENALTY=3.1460
8911
PRIMER_LEFT_PENALTY=2.478252
8912
PRIMER_RIGHT_PENALTY=0.667795
8913
PRIMER_LEFT_SEQUENCE=TCCTCTGTAGAAAATGCACTGC
8914
PRIMER_RIGHT_SEQUENCE=CAGACCCATGGAGAGTGGAG
8915
PRIMER_LEFT=23,22
8916
PRIMER_RIGHT=334,20
8917
PRIMER_LEFT_TM=59.522
8918
PRIMER_RIGHT_TM=60.668
8919
PRIMER_LEFT_GC_PERCENT=45.455
8920
PRIMER_RIGHT_GC_PERCENT=60.000
8921
PRIMER_LEFT_SELF_ANY=5.00
8922
PRIMER_RIGHT_SELF_ANY=6.00
8923
PRIMER_LEFT_SELF_END=5.00
8924
PRIMER_RIGHT_SELF_END=0.00
8925
PRIMER_LEFT_END_STABILITY=7.9000
8926
PRIMER_RIGHT_END_STABILITY=8.2000
8927
PRIMER_PAIR_COMPL_ANY=4.00
8928
PRIMER_PAIR_COMPL_END=2.00
8929
PRIMER_PRODUCT_SIZE=312
8930
PRIMER_PAIR_PENALTY_1=3.4888
8931
PRIMER_LEFT_1_PENALTY=2.478252
8932
PRIMER_RIGHT_1_PENALTY=1.010529
8933
PRIMER_LEFT_1_SEQUENCE=TCCTCTGTAGAAAATGCACTGC
8934
PRIMER_RIGHT_1_SEQUENCE=TCGCTTTTGAGCTGAAAATGT
8935
PRIMER_LEFT_1=23,22
8936
PRIMER_RIGHT_1=303,21
8937
PRIMER_LEFT_1_TM=59.522
8938
PRIMER_RIGHT_1_TM=60.011
8939
PRIMER_LEFT_1_GC_PERCENT=45.455
8940
PRIMER_RIGHT_1_GC_PERCENT=38.095
8941
PRIMER_LEFT_1_SELF_ANY=5.00
8942
PRIMER_RIGHT_1_SELF_ANY=10.00
8943
PRIMER_LEFT_1_SELF_END=5.00
8944
PRIMER_RIGHT_1_SELF_END=6.00
8945
PRIMER_LEFT_1_END_STABILITY=7.9000
8946
PRIMER_RIGHT_1_END_STABILITY=6.6000
8947
PRIMER_PAIR_1_COMPL_ANY=4.00
8948
PRIMER_PAIR_1_COMPL_END=1.00
8949
PRIMER_PRODUCT_SIZE_1=281
8950
PRIMER_PAIR_PENALTY_2=3.7565
8951
PRIMER_LEFT_2_PENALTY=3.088662
8952
PRIMER_RIGHT_2_PENALTY=0.667795
8953
PRIMER_LEFT_2_SEQUENCE=TGCACTGCTAATAACTTTGCTCA
8954
PRIMER_RIGHT_2_SEQUENCE=CAGACCCATGGAGAGTGGAG
8955
PRIMER_LEFT_2=37,23
8956
PRIMER_RIGHT_2=334,20
8957
PRIMER_LEFT_2_TM=60.089
8958
PRIMER_RIGHT_2_TM=60.668
8959
PRIMER_LEFT_2_GC_PERCENT=39.130
8960
PRIMER_RIGHT_2_GC_PERCENT=60.000
8961
PRIMER_LEFT_2_SELF_ANY=5.00
8962
PRIMER_RIGHT_2_SELF_ANY=6.00
8963
PRIMER_LEFT_2_SELF_END=2.00
8964
PRIMER_RIGHT_2_SELF_END=0.00
8965
PRIMER_LEFT_2_END_STABILITY=8.2000
8966
PRIMER_RIGHT_2_END_STABILITY=8.2000
8967
PRIMER_PAIR_2_COMPL_ANY=4.00
8968
PRIMER_PAIR_2_COMPL_END=2.00
8969
PRIMER_PRODUCT_SIZE_2=298
8970
PRIMER_PAIR_PENALTY_3=3.7893
8971
PRIMER_LEFT_3_PENALTY=3.121486
8972
PRIMER_RIGHT_3_PENALTY=0.667795
8973
PRIMER_LEFT_3_SEQUENCE=TCTTAATATGGCTCCAGGTAGCA
8974
PRIMER_RIGHT_3_SEQUENCE=CAGACCCATGGAGAGTGGAG
8975
PRIMER_LEFT_3=78,23
8976
PRIMER_RIGHT_3=334,20
8977
PRIMER_LEFT_3_TM=60.121
8978
PRIMER_RIGHT_3_TM=60.668
8979
PRIMER_LEFT_3_GC_PERCENT=43.478
8980
PRIMER_RIGHT_3_GC_PERCENT=60.000
8981
PRIMER_LEFT_3_SELF_ANY=5.00
8982
PRIMER_RIGHT_3_SELF_ANY=6.00
8983
PRIMER_LEFT_3_SELF_END=3.00
8984
PRIMER_RIGHT_3_SELF_END=0.00
8985
PRIMER_LEFT_3_END_STABILITY=7.5000
8986
PRIMER_RIGHT_3_END_STABILITY=8.2000
8987
PRIMER_PAIR_3_COMPL_ANY=5.00
8988
PRIMER_PAIR_3_COMPL_END=2.00
8989
PRIMER_PRODUCT_SIZE_3=257
8990
PRIMER_PAIR_PENALTY_4=3.8159
8991
PRIMER_LEFT_4_PENALTY=3.148084
8992
PRIMER_RIGHT_4_PENALTY=0.667795
8993
PRIMER_LEFT_4_SEQUENCE=AATATGGCTCCAGGTAGCAGT
8994
PRIMER_RIGHT_4_SEQUENCE=CAGACCCATGGAGAGTGGAG
8995
PRIMER_LEFT_4=82,21
8996
PRIMER_RIGHT_4=334,20
8997
PRIMER_LEFT_4_TM=57.852
8998
PRIMER_RIGHT_4_TM=60.668
8999
PRIMER_LEFT_4_GC_PERCENT=47.619
9000
PRIMER_RIGHT_4_GC_PERCENT=60.000
9001
PRIMER_LEFT_4_SELF_ANY=5.00
9002
PRIMER_RIGHT_4_SELF_ANY=6.00
9003
PRIMER_LEFT_4_SELF_END=3.00
9004
PRIMER_RIGHT_4_SELF_END=0.00
9005
PRIMER_LEFT_4_END_STABILITY=7.9000
9006
PRIMER_RIGHT_4_END_STABILITY=8.2000
9007
PRIMER_PAIR_4_COMPL_ANY=5.00
9008
PRIMER_PAIR_4_COMPL_END=1.00
9009
PRIMER_PRODUCT_SIZE_4=253
9010
=
9011
MARKER_NAME=45.RHAP64FG12.seq
9012
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNCTGTCAATATTCCAGAACAGCGCATGCCACCCACACAACGTGCTTCAGCCAGCCCTGCAGTGCCCTTCCTGGACAGGATTTCTTCTTCTTTGAAAGAAAAACAACAACAAAGCTTTTATTATANNTATCNAATTNNNNNNNNNNNNNNTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTGTATTTGGACATAAAGATGAGACTGGTAGGAAGCTTTGTGTTGGAGACTGTCCTTAAATTCCCTCCATCTTTCCTTCAGGGGAATAGCCCATGCCTGTGGAACCCATCGCTCCTCACTGGGTAGCTGTCAGAAGTGTGAGGATGCTAACAGTCTGTAAACGGTGCTGTCCCTGCAGCGATCCCCAGCAGAGTCTGAGAAACTCCCAATCCACCAGCAAGATGACACAATTGTTTTGCTG
9013
INCLUDED_REGION=22,442
9014
TARGET=156,14,TG
9015
TARGET=172,50,TG
9016
PRIMER_COMMENT=CL is TTGAAAGAAAAACAACAACAAAGC
9017
PRIMER_COMMENT=CR is CCAACACAAAGCTTCCTACCA
9018
PRIMER_PAIR_PENALTY=5.4191
9019
PRIMER_LEFT_PENALTY=4.265519
9020
PRIMER_RIGHT_PENALTY=1.153589
9021
PRIMER_LEFT_SEQUENCE=TTGAAAGAAAAACAACAACAAAGC
9022
PRIMER_RIGHT_SEQUENCE=CCAACACAAAGCTTCCTACCA
9023
PRIMER_LEFT=111,24
9024
PRIMER_RIGHT=268,21
9025
PRIMER_LEFT_TM=59.734
9026
PRIMER_RIGHT_TM=60.154
9027
PRIMER_LEFT_GC_PERCENT=29.167
9028
PRIMER_RIGHT_GC_PERCENT=47.619
9029
PRIMER_LEFT_SELF_ANY=3.00
9030
PRIMER_RIGHT_SELF_ANY=6.00
9031
PRIMER_LEFT_SELF_END=2.00
9032
PRIMER_RIGHT_SELF_END=0.00
9033
PRIMER_LEFT_END_STABILITY=8.5000
9034
PRIMER_RIGHT_END_STABILITY=7.2000
9035
PRIMER_PAIR_COMPL_ANY=4.00
9036
PRIMER_PAIR_COMPL_END=0.00
9037
PRIMER_PRODUCT_SIZE=158
9038
PRIMER_PAIR_PENALTY_1=5.5055
9039
PRIMER_LEFT_1_PENALTY=4.265519
9040
PRIMER_RIGHT_1_PENALTY=1.239976
9041
PRIMER_LEFT_1_SEQUENCE=TTGAAAGAAAAACAACAACAAAGC
9042
PRIMER_RIGHT_1_SEQUENCE=GGACAGTCTCCAACACAAAGC
9043
PRIMER_LEFT_1=111,24
9044
PRIMER_RIGHT_1=277,21
9045
PRIMER_LEFT_1_TM=59.734
9046
PRIMER_RIGHT_1_TM=59.760
9047
PRIMER_LEFT_1_GC_PERCENT=29.167
9048
PRIMER_RIGHT_1_GC_PERCENT=52.381
9049
PRIMER_LEFT_1_SELF_ANY=3.00
9050
PRIMER_RIGHT_1_SELF_ANY=5.00
9051
PRIMER_LEFT_1_SELF_END=2.00
9052
PRIMER_RIGHT_1_SELF_END=2.00
9053
PRIMER_LEFT_1_END_STABILITY=8.5000
9054
PRIMER_RIGHT_1_END_STABILITY=8.5000
9055
PRIMER_PAIR_1_COMPL_ANY=3.00
9056
PRIMER_PAIR_1_COMPL_END=2.00
9057
PRIMER_PRODUCT_SIZE_1=167
9058
PRIMER_PAIR_PENALTY_2=5.5709
9059
PRIMER_LEFT_2_PENALTY=0.821928
9060
PRIMER_RIGHT_2_PENALTY=4.748938
9061
PRIMER_LEFT_2_SEQUENCE=CCCTTCCTGGACAGGATTTC
9062
PRIMER_RIGHT_2_SEQUENCE=CCAGTCTCATCTTTATGTCCAAA
9063
PRIMER_LEFT_2=84,20
9064
PRIMER_RIGHT_2=250,23
9065
PRIMER_LEFT_2_TM=60.822
9066
PRIMER_RIGHT_2_TM=58.251
9067
PRIMER_LEFT_2_GC_PERCENT=55.000
9068
PRIMER_RIGHT_2_GC_PERCENT=39.130
9069
PRIMER_LEFT_2_SELF_ANY=8.00
9070
PRIMER_RIGHT_2_SELF_ANY=3.00
9071
PRIMER_LEFT_2_SELF_END=2.00
9072
PRIMER_RIGHT_2_SELF_END=2.00
9073
PRIMER_LEFT_2_END_STABILITY=6.9000
9074
PRIMER_RIGHT_2_END_STABILITY=8.8000
9075
PRIMER_PAIR_2_COMPL_ANY=6.00
9076
PRIMER_PAIR_2_COMPL_END=2.00
9077
PRIMER_PRODUCT_SIZE_2=167
9078
PRIMER_PAIR_PENALTY_3=5.6387
9079
PRIMER_LEFT_3_PENALTY=4.485142
9080
PRIMER_RIGHT_3_PENALTY=1.153589
9081
PRIMER_LEFT_3_SEQUENCE=TGAAAGAAAAACAACAACAAAGC
9082
PRIMER_RIGHT_3_SEQUENCE=CCAACACAAAGCTTCCTACCA
9083
PRIMER_LEFT_3=112,23
9084
PRIMER_RIGHT_3=268,21
9085
PRIMER_LEFT_3_TM=58.515
9086
PRIMER_RIGHT_3_TM=60.154
9087
PRIMER_LEFT_3_GC_PERCENT=30.435
9088
PRIMER_RIGHT_3_GC_PERCENT=47.619
9089
PRIMER_LEFT_3_SELF_ANY=2.00
9090
PRIMER_RIGHT_3_SELF_ANY=6.00
9091
PRIMER_LEFT_3_SELF_END=2.00
9092
PRIMER_RIGHT_3_SELF_END=0.00
9093
PRIMER_LEFT_3_END_STABILITY=8.5000
9094
PRIMER_RIGHT_3_END_STABILITY=7.2000
9095
PRIMER_PAIR_3_COMPL_ANY=4.00
9096
PRIMER_PAIR_3_COMPL_END=0.00
9097
PRIMER_PRODUCT_SIZE_3=157
9098
PRIMER_PAIR_PENALTY_4=5.7251
9099
PRIMER_LEFT_4_PENALTY=4.485142
9100
PRIMER_RIGHT_4_PENALTY=1.239976
9101
PRIMER_LEFT_4_SEQUENCE=TGAAAGAAAAACAACAACAAAGC
9102
PRIMER_RIGHT_4_SEQUENCE=GGACAGTCTCCAACACAAAGC
9103
PRIMER_LEFT_4=112,23
9104
PRIMER_RIGHT_4=277,21
9105
PRIMER_LEFT_4_TM=58.515
9106
PRIMER_RIGHT_4_TM=59.760
9107
PRIMER_LEFT_4_GC_PERCENT=30.435
9108
PRIMER_RIGHT_4_GC_PERCENT=52.381
9109
PRIMER_LEFT_4_SELF_ANY=2.00
9110
PRIMER_RIGHT_4_SELF_ANY=5.00
9111
PRIMER_LEFT_4_SELF_END=2.00
9112
PRIMER_RIGHT_4_SELF_END=2.00
9113
PRIMER_LEFT_4_END_STABILITY=8.5000
9114
PRIMER_RIGHT_4_END_STABILITY=8.5000
9115
PRIMER_PAIR_4_COMPL_ANY=3.00
9116
PRIMER_PAIR_4_COMPL_END=2.00
9117
PRIMER_PRODUCT_SIZE_4=166
9118
=
9119
MARKER_NAME=46.RHAP64FH10.seq
9120
SEQUENCE=NNNNNNNNNNNNCCCAGCCCTGCTAACCACCTAAAGGCACCAGCAGGAGCTNCCCTCAAAAGATCTCACGTTTCCTCTGACAGCACCTTAAAGGAGCACCANTAATGAAGGCTGCTCTGTTGNGTGGGTAGGATGCCAAGGCAGAGGCGGCTTTCTACAGAGCTCCTTCCTCGCCGCAGTAACTCAAGTAGACACCTCAGTCGTTCTCCTGCAGTGTTCTGTGTTCATCACAAGGGGGCAGCCTTGACCCACTTCCCATGGTTGCCAATTCTCCAACCCATCCCCCTCCCCTCATCTAAGAAGACCTTGAACCTGGAGGAGAATATAAATAAACCTGAAAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNGANNNNNNNNNNNNNNNNNNNNATGNGTNTGNNTGGTTAGNGTAAANATGGGGTATGNNNNNNTGGNGNGTTAGANNNANGGTGAGNNTATNTGNATGT
9121
INCLUDED_REGION=12,456
9122
TARGET=343,26,GT
9123
TARGET=371,20,TG
9124
PRIMER_COMMENT=CL is
9125
PRIMER_COMMENT=CR is
9126
=
9127
MARKER_NAME=46.RHAP64FH4.seq
9128
SEQUENCE=NNNNNNNNNNNNNNNNNNNCCAGCTAGCCTGGCACACACAACAAAACAAGAACCCTTGCTTGAATAAAATGGAAGGTGAGGTCTGATACCCAAAATTAACCTTTAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGNTATTTTTATAANATAGAGAAAAGNAATTTCATTAAAAGGAANTGAAAGAAGTNTAAATATAATTATANATTTTAGATTAAAANTTTGTAATNAAAGCACACGGTATGTGTTTATTTAAGTGAGAGTACACGTGGAGTTAAAATGTGGATATANATAAAAATNAGNGAGNTTTTAAAAATNANTTTCTTAGNTTGNTTTAGGGATATNTNTNNACCACAAACANATACANAAAACCCAAAANAAANNNNNNNNNNNNNNNNNAGNA
9129
INCLUDED_REGION=19,433
9130
TARGET=107,18,TC
9131
TARGET=126,22,TN
9132
TARGET=151,32,AC
9133
TARGET=433,15,GA
9134
PRIMER_COMMENT=CL is CCTGGCACACACAACAAAAC
9135
PRIMER_COMMENT=CR is TCCACATTTTAACTCCACGTG
9136
PRIMER_PAIR_PENALTY=2.5085
9137
PRIMER_LEFT_PENALTY=0.048588
9138
PRIMER_RIGHT_PENALTY=2.459929
9139
PRIMER_LEFT_SEQUENCE=CCTGGCACACACAACAAAAC
9140
PRIMER_RIGHT_SEQUENCE=TCCACATTTTAACTCCACGTG
9141
PRIMER_LEFT=27,20
9142
PRIMER_RIGHT=334,21
9143
PRIMER_LEFT_TM=60.049
9144
PRIMER_RIGHT_TM=58.540
9145
PRIMER_LEFT_GC_PERCENT=50.000
9146
PRIMER_RIGHT_GC_PERCENT=42.857
9147
PRIMER_LEFT_SELF_ANY=3.00
9148
PRIMER_RIGHT_SELF_ANY=6.00
9149
PRIMER_LEFT_SELF_END=0.00
9150
PRIMER_RIGHT_SELF_END=6.00
9151
PRIMER_LEFT_END_STABILITY=7.0000
9152
PRIMER_RIGHT_END_STABILITY=8.1000
9153
PRIMER_PAIR_COMPL_ANY=4.00
9154
PRIMER_PAIR_COMPL_END=1.00
9155
PRIMER_PRODUCT_SIZE=308
9156
PRIMER_PAIR_PENALTY_1=2.6473
9157
PRIMER_LEFT_1_PENALTY=0.048588
9158
PRIMER_RIGHT_1_PENALTY=2.598673
9159
PRIMER_LEFT_1_SEQUENCE=CCTGGCACACACAACAAAAC
9160
PRIMER_RIGHT_1_SEQUENCE=TCCACATTTTAACTCCACGTGT
9161
PRIMER_LEFT_1=27,20
9162
PRIMER_RIGHT_1=334,22
9163
PRIMER_LEFT_1_TM=60.049
9164
PRIMER_RIGHT_1_TM=59.401
9165
PRIMER_LEFT_1_GC_PERCENT=50.000
9166
PRIMER_RIGHT_1_GC_PERCENT=40.909
9167
PRIMER_LEFT_1_SELF_ANY=3.00
9168
PRIMER_RIGHT_1_SELF_ANY=6.00
9169
PRIMER_LEFT_1_SELF_END=0.00
9170
PRIMER_RIGHT_1_SELF_END=4.00
9171
PRIMER_LEFT_1_END_STABILITY=7.0000
9172
PRIMER_RIGHT_1_END_STABILITY=8.1000
9173
PRIMER_PAIR_1_COMPL_ANY=4.00
9174
PRIMER_PAIR_1_COMPL_END=2.00
9175
PRIMER_PRODUCT_SIZE_1=308
9176
PRIMER_PAIR_PENALTY_2=2.8512
9177
PRIMER_LEFT_2_PENALTY=0.391259
9178
PRIMER_RIGHT_2_PENALTY=2.459929
9179
PRIMER_LEFT_2_SEQUENCE=TGGCACACACAACAAAACAA
9180
PRIMER_RIGHT_2_SEQUENCE=TCCACATTTTAACTCCACGTG
9181
PRIMER_LEFT_2=29,20
9182
PRIMER_RIGHT_2=334,21
9183
PRIMER_LEFT_2_TM=59.609
9184
PRIMER_RIGHT_2_TM=58.540
9185
PRIMER_LEFT_2_GC_PERCENT=40.000
9186
PRIMER_RIGHT_2_GC_PERCENT=42.857
9187
PRIMER_LEFT_2_SELF_ANY=3.00
9188
PRIMER_RIGHT_2_SELF_ANY=6.00
9189
PRIMER_LEFT_2_SELF_END=0.00
9190
PRIMER_RIGHT_2_SELF_END=6.00
9191
PRIMER_LEFT_2_END_STABILITY=7.0000
9192
PRIMER_RIGHT_2_END_STABILITY=8.1000
9193
PRIMER_PAIR_2_COMPL_ANY=4.00
9194
PRIMER_PAIR_2_COMPL_END=1.00
9195
PRIMER_PRODUCT_SIZE_2=306
9196
PRIMER_PAIR_PENALTY_3=2.9899
9197
PRIMER_LEFT_3_PENALTY=0.391259
9198
PRIMER_RIGHT_3_PENALTY=2.598673
9199
PRIMER_LEFT_3_SEQUENCE=TGGCACACACAACAAAACAA
9200
PRIMER_RIGHT_3_SEQUENCE=TCCACATTTTAACTCCACGTGT
9201
PRIMER_LEFT_3=29,20
9202
PRIMER_RIGHT_3=334,22
9203
PRIMER_LEFT_3_TM=59.609
9204
PRIMER_RIGHT_3_TM=59.401
9205
PRIMER_LEFT_3_GC_PERCENT=40.000
9206
PRIMER_RIGHT_3_GC_PERCENT=40.909
9207
PRIMER_LEFT_3_SELF_ANY=3.00
9208
PRIMER_RIGHT_3_SELF_ANY=6.00
9209
PRIMER_LEFT_3_SELF_END=0.00
9210
PRIMER_RIGHT_3_SELF_END=4.00
9211
PRIMER_LEFT_3_END_STABILITY=7.0000
9212
PRIMER_RIGHT_3_END_STABILITY=8.1000
9213
PRIMER_PAIR_3_COMPL_ANY=4.00
9214
PRIMER_PAIR_3_COMPL_END=2.00
9215
PRIMER_PRODUCT_SIZE_3=306
9216
PRIMER_PAIR_PENALTY_4=3.1664
9217
PRIMER_LEFT_4_PENALTY=0.048588
9218
PRIMER_RIGHT_4_PENALTY=3.117810
9219
PRIMER_LEFT_4_SEQUENCE=CCTGGCACACACAACAAAAC
9220
PRIMER_RIGHT_4_SEQUENCE=ATCCACATTTTAACTCCACGTG
9221
PRIMER_LEFT_4=27,20
9222
PRIMER_RIGHT_4=335,22
9223
PRIMER_LEFT_4_TM=60.049
9224
PRIMER_RIGHT_4_TM=58.882
9225
PRIMER_LEFT_4_GC_PERCENT=50.000
9226
PRIMER_RIGHT_4_GC_PERCENT=40.909
9227
PRIMER_LEFT_4_SELF_ANY=3.00
9228
PRIMER_RIGHT_4_SELF_ANY=6.00
9229
PRIMER_LEFT_4_SELF_END=0.00
9230
PRIMER_RIGHT_4_SELF_END=6.00
9231
PRIMER_LEFT_4_END_STABILITY=7.0000
9232
PRIMER_RIGHT_4_END_STABILITY=8.1000
9233
PRIMER_PAIR_4_COMPL_ANY=4.00
9234
PRIMER_PAIR_4_COMPL_END=1.00
9235
PRIMER_PRODUCT_SIZE_4=309
9236
=
9237
MARKER_NAME=06.RHAP68FB3.seq
9238
SEQUENCE=NNNNNNNNACACTTGCTTTTGGTAGGTAAAACGCATGGAATTTGATTCCAAACATGACACAAAAGTTCACCTTATTTCCAACTCATTTCATGCACTTGTGTAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTAAACCTTTGGGTTTAGCTACATTTAGTTGGTTCTGTTCCTTGCAACTGAAACATTGAGATTTTTCCCACAAGGAAAGAAAGTAAATGGTGGGA
9239
INCLUDED_REGION=8,243
9240
TARGET=104,52,AC
9241
PRIMER_COMMENT=CL is GCATGGAATTTGATTCCAAA
9242
PRIMER_COMMENT=CR is TTGCAAGGAACAGAACCAACT
9243
PRIMER_PAIR_PENALTY=2.8306
9244
PRIMER_LEFT_PENALTY=1.596296
9245
PRIMER_RIGHT_PENALTY=1.234342
9246
PRIMER_LEFT_SEQUENCE=GCATGGAATTTGATTCCAAA
9247
PRIMER_RIGHT_SEQUENCE=AGTTGCAAGGAACAGAACCAA
9248
PRIMER_LEFT=32,20
9249
PRIMER_RIGHT=204,21
9250
PRIMER_LEFT_TM=58.404
9251
PRIMER_RIGHT_TM=59.766
9252
PRIMER_LEFT_GC_PERCENT=35.000
9253
PRIMER_RIGHT_GC_PERCENT=42.857
9254
PRIMER_LEFT_SELF_ANY=9.00
9255
PRIMER_RIGHT_SELF_ANY=6.00
9256
PRIMER_LEFT_SELF_END=5.00
9257
PRIMER_RIGHT_SELF_END=0.00
9258
PRIMER_LEFT_END_STABILITY=8.8000
9259
PRIMER_RIGHT_END_STABILITY=8.2000
9260
PRIMER_PAIR_COMPL_ANY=5.00
9261
PRIMER_PAIR_COMPL_END=1.00
9262
PRIMER_PRODUCT_SIZE=173
9263
PRIMER_PAIR_PENALTY_1=2.8306
9264
PRIMER_LEFT_1_PENALTY=1.596296
9265
PRIMER_RIGHT_1_PENALTY=1.234342
9266
PRIMER_LEFT_1_SEQUENCE=GCATGGAATTTGATTCCAAA
9267
PRIMER_RIGHT_1_SEQUENCE=TTGCAAGGAACAGAACCAACT
9268
PRIMER_LEFT_1=32,20
9269
PRIMER_RIGHT_1=202,21
9270
PRIMER_LEFT_1_TM=58.404
9271
PRIMER_RIGHT_1_TM=59.766
9272
PRIMER_LEFT_1_GC_PERCENT=35.000
9273
PRIMER_RIGHT_1_GC_PERCENT=42.857
9274
PRIMER_LEFT_1_SELF_ANY=9.00
9275
PRIMER_RIGHT_1_SELF_ANY=6.00
9276
PRIMER_LEFT_1_SELF_END=5.00
9277
PRIMER_RIGHT_1_SELF_END=1.00
9278
PRIMER_LEFT_1_END_STABILITY=8.8000
9279
PRIMER_RIGHT_1_END_STABILITY=6.7000
9280
PRIMER_PAIR_1_COMPL_ANY=5.00
9281
PRIMER_PAIR_1_COMPL_END=1.00
9282
PRIMER_PRODUCT_SIZE_1=171
9283
PRIMER_PAIR_PENALTY_2=2.8478
9284
PRIMER_LEFT_2_PENALTY=1.596296
9285
PRIMER_RIGHT_2_PENALTY=1.251471
9286
PRIMER_LEFT_2_SEQUENCE=GCATGGAATTTGATTCCAAA
9287
PRIMER_RIGHT_2_SEQUENCE=TTGCAAGGAACAGAACCAAC
9288
PRIMER_LEFT_2=32,20
9289
PRIMER_RIGHT_2=202,20
9290
PRIMER_LEFT_2_TM=58.404
9291
PRIMER_RIGHT_2_TM=58.749
9292
PRIMER_LEFT_2_GC_PERCENT=35.000
9293
PRIMER_RIGHT_2_GC_PERCENT=45.000
9294
PRIMER_LEFT_2_SELF_ANY=9.00
9295
PRIMER_RIGHT_2_SELF_ANY=6.00
9296
PRIMER_LEFT_2_SELF_END=5.00
9297
PRIMER_RIGHT_2_SELF_END=0.00
9298
PRIMER_LEFT_2_END_STABILITY=8.8000
9299
PRIMER_RIGHT_2_END_STABILITY=8.2000
9300
PRIMER_PAIR_2_COMPL_ANY=5.00
9301
PRIMER_PAIR_2_COMPL_END=0.00
9302
PRIMER_PRODUCT_SIZE_2=171
9303
PRIMER_PAIR_PENALTY_3=2.8478
9304
PRIMER_LEFT_3_PENALTY=1.596296
9305
PRIMER_RIGHT_3_PENALTY=1.251471
9306
PRIMER_LEFT_3_SEQUENCE=GCATGGAATTTGATTCCAAA
9307
PRIMER_RIGHT_3_SEQUENCE=GTTGCAAGGAACAGAACCAA
9308
PRIMER_LEFT_3=32,20
9309
PRIMER_RIGHT_3=203,20
9310
PRIMER_LEFT_3_TM=58.404
9311
PRIMER_RIGHT_3_TM=58.749
9312
PRIMER_LEFT_3_GC_PERCENT=35.000
9313
PRIMER_RIGHT_3_GC_PERCENT=45.000
9314
PRIMER_LEFT_3_SELF_ANY=9.00
9315
PRIMER_RIGHT_3_SELF_ANY=6.00
9316
PRIMER_LEFT_3_SELF_END=5.00
9317
PRIMER_RIGHT_3_SELF_END=0.00
9318
PRIMER_LEFT_3_END_STABILITY=8.8000
9319
PRIMER_RIGHT_3_END_STABILITY=8.2000
9320
PRIMER_PAIR_3_COMPL_ANY=5.00
9321
PRIMER_PAIR_3_COMPL_END=1.00
9322
PRIMER_PRODUCT_SIZE_3=172
9323
PRIMER_PAIR_PENALTY_4=2.8800
9324
PRIMER_LEFT_4_PENALTY=1.596296
9325
PRIMER_RIGHT_4_PENALTY=1.283723
9326
PRIMER_LEFT_4_SEQUENCE=GCATGGAATTTGATTCCAAA
9327
PRIMER_RIGHT_4_SEQUENCE=TCAGTTGCAAGGAACAGAACC
9328
PRIMER_LEFT_4=32,20
9329
PRIMER_RIGHT_4=206,21
9330
PRIMER_LEFT_4_TM=58.404
9331
PRIMER_RIGHT_4_TM=60.284
9332
PRIMER_LEFT_4_GC_PERCENT=35.000
9333
PRIMER_RIGHT_4_GC_PERCENT=47.619
9334
PRIMER_LEFT_4_SELF_ANY=9.00
9335
PRIMER_RIGHT_4_SELF_ANY=6.00
9336
PRIMER_LEFT_4_SELF_END=5.00
9337
PRIMER_RIGHT_4_SELF_END=1.00
9338
PRIMER_LEFT_4_END_STABILITY=8.8000
9339
PRIMER_RIGHT_4_END_STABILITY=7.9000
9340
PRIMER_PAIR_4_COMPL_ANY=4.00
9341
PRIMER_PAIR_4_COMPL_END=0.00
9342
PRIMER_PRODUCT_SIZE_4=175
9343
=
9344
MARKER_NAME=07.RHAP68FC1.seq
9345
SEQUENCE=NNNNNNNNNNNNNNNNNNNAGGTAGATGATTAGAGAGAACAATCTATTTGGGGTGGATTATTGAGAATGAGAAAACCATTAGAAATGCCAGTAAAACTTCAAATTTGATTACATCTTCATCATCATCATATATTTGTGTGCATGCAAGTGCTCGTAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGCCAAAAATCAATAAAATATGCAGTAACTCTTTGTGACTTAGAATTTGGTNNNNNNNNNNN
9346
INCLUDED_REGION=19,230
9347
TARGET=157,40,GT
9348
PRIMER_COMMENT=CL is TGGGGTGGATTATTGAGAATG
9349
PRIMER_COMMENT=CR is TGCATATTTTATTGATTTTTGGCA
9350
PRIMER_PAIR_PENALTY=5.8932
9351
PRIMER_LEFT_PENALTY=1.369105
9352
PRIMER_RIGHT_PENALTY=4.524067
9353
PRIMER_LEFT_SEQUENCE=TGGGGTGGATTATTGAGAATG
9354
PRIMER_RIGHT_SEQUENCE=TGCATATTTTATTGATTTTTGGCA
9355
PRIMER_LEFT=48,21
9356
PRIMER_RIGHT=221,24
9357
PRIMER_LEFT_TM=59.631
9358
PRIMER_RIGHT_TM=60.524
9359
PRIMER_LEFT_GC_PERCENT=42.857
9360
PRIMER_RIGHT_GC_PERCENT=25.000
9361
PRIMER_LEFT_SELF_ANY=4.00
9362
PRIMER_RIGHT_SELF_ANY=4.00
9363
PRIMER_LEFT_SELF_END=2.00
9364
PRIMER_RIGHT_SELF_END=3.00
9365
PRIMER_LEFT_END_STABILITY=6.9000
9366
PRIMER_RIGHT_END_STABILITY=10.0000
9367
PRIMER_PAIR_COMPL_ANY=3.00
9368
PRIMER_PAIR_COMPL_END=2.00
9369
PRIMER_PRODUCT_SIZE=174
9370
PRIMER_PAIR_PENALTY_1=6.2152
9371
PRIMER_LEFT_1_PENALTY=1.369105
9372
PRIMER_RIGHT_1_PENALTY=4.846138
9373
PRIMER_LEFT_1_SEQUENCE=TGGGGTGGATTATTGAGAATG
9374
PRIMER_RIGHT_1_SEQUENCE=TGCATATTTTATTGATTTTTGGC
9375
PRIMER_LEFT_1=48,21
9376
PRIMER_RIGHT_1=221,23
9377
PRIMER_LEFT_1_TM=59.631
9378
PRIMER_RIGHT_1_TM=58.154
9379
PRIMER_LEFT_1_GC_PERCENT=42.857
9380
PRIMER_RIGHT_1_GC_PERCENT=26.087
9381
PRIMER_LEFT_1_SELF_ANY=4.00
9382
PRIMER_RIGHT_1_SELF_ANY=4.00
9383
PRIMER_LEFT_1_SELF_END=2.00
9384
PRIMER_RIGHT_1_SELF_END=2.00
9385
PRIMER_LEFT_1_END_STABILITY=6.9000
9386
PRIMER_RIGHT_1_END_STABILITY=10.0000
9387
PRIMER_PAIR_1_COMPL_ANY=3.00
9388
PRIMER_PAIR_1_COMPL_END=1.00
9389
PRIMER_PRODUCT_SIZE_1=174
9390
PRIMER_PAIR_PENALTY_2=6.2152
9391
PRIMER_LEFT_2_PENALTY=1.369105
9392
PRIMER_RIGHT_2_PENALTY=4.846138
9393
PRIMER_LEFT_2_SEQUENCE=TGGGGTGGATTATTGAGAATG
9394
PRIMER_RIGHT_2_SEQUENCE=GCATATTTTATTGATTTTTGGCA
9395
PRIMER_LEFT_2=48,21
9396
PRIMER_RIGHT_2=220,23
9397
PRIMER_LEFT_2_TM=59.631
9398
PRIMER_RIGHT_2_TM=58.154
9399
PRIMER_LEFT_2_GC_PERCENT=42.857
9400
PRIMER_RIGHT_2_GC_PERCENT=26.087
9401
PRIMER_LEFT_2_SELF_ANY=4.00
9402
PRIMER_RIGHT_2_SELF_ANY=4.00
9403
PRIMER_LEFT_2_SELF_END=2.00
9404
PRIMER_RIGHT_2_SELF_END=3.00
9405
PRIMER_LEFT_2_END_STABILITY=6.9000
9406
PRIMER_RIGHT_2_END_STABILITY=10.0000
9407
PRIMER_PAIR_2_COMPL_ANY=3.00
9408
PRIMER_PAIR_2_COMPL_END=2.00
9409
PRIMER_PRODUCT_SIZE_2=173
9410
PRIMER_PAIR_PENALTY_3=6.3549
9411
PRIMER_LEFT_3_PENALTY=1.369105
9412
PRIMER_RIGHT_3_PENALTY=4.985768
9413
PRIMER_LEFT_3_SEQUENCE=TGGGGTGGATTATTGAGAATG
9414
PRIMER_RIGHT_3_SEQUENCE=CTGCATATTTTATTGATTTTTGGC
9415
PRIMER_LEFT_3=48,21
9416
PRIMER_RIGHT_3=222,24
9417
PRIMER_LEFT_3_TM=59.631
9418
PRIMER_RIGHT_3_TM=59.014
9419
PRIMER_LEFT_3_GC_PERCENT=42.857
9420
PRIMER_RIGHT_3_GC_PERCENT=29.167
9421
PRIMER_LEFT_3_SELF_ANY=4.00
9422
PRIMER_RIGHT_3_SELF_ANY=4.00
9423
PRIMER_LEFT_3_SELF_END=2.00
9424
PRIMER_RIGHT_3_SELF_END=2.00
9425
PRIMER_LEFT_3_END_STABILITY=6.9000
9426
PRIMER_RIGHT_3_END_STABILITY=10.0000
9427
PRIMER_PAIR_3_COMPL_ANY=3.00
9428
PRIMER_PAIR_3_COMPL_END=1.00
9429
PRIMER_PRODUCT_SIZE_3=175
9430
PRIMER_PAIR_PENALTY_4=6.5865
9431
PRIMER_LEFT_4_PENALTY=1.740315
9432
PRIMER_RIGHT_4_PENALTY=4.846138
9433
PRIMER_LEFT_4_SEQUENCE=TTTGGGGTGGATTATTGAGAA
9434
PRIMER_RIGHT_4_SEQUENCE=GCATATTTTATTGATTTTTGGCA
9435
PRIMER_LEFT_4=46,21
9436
PRIMER_RIGHT_4=220,23
9437
PRIMER_LEFT_4_TM=59.260
9438
PRIMER_RIGHT_4_TM=58.154
9439
PRIMER_LEFT_4_GC_PERCENT=38.095
9440
PRIMER_RIGHT_4_GC_PERCENT=26.087
9441
PRIMER_LEFT_4_SELF_ANY=3.00
9442
PRIMER_RIGHT_4_SELF_ANY=4.00
9443
PRIMER_LEFT_4_SELF_END=2.00
9444
PRIMER_RIGHT_4_SELF_END=3.00
9445
PRIMER_LEFT_4_END_STABILITY=6.7000
9446
PRIMER_RIGHT_4_END_STABILITY=10.0000
9447
PRIMER_PAIR_4_COMPL_ANY=3.00
9448
PRIMER_PAIR_4_COMPL_END=2.00
9449
PRIMER_PRODUCT_SIZE_4=175
9450
=
9451
MARKER_NAME=08.RHAP68FC2.seq
9452
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATACATGCATAACCCATTCCTGAATCCATCTGCTGTCAAGCACACAACCCCGTGGCAGAAGCCTAGGGACCAGACAGGAAGCTTCTCCTGAGCTTCCCTGTGGCTGTGGCTAGCAGGAGGCCAGAAGGTCAGCATGGTGGTCCTGTGGGGGAGACACTGCAACCCAGCTAAGCTTCAGCTTGACTCCTGTTNNNNNNNN
9453
INCLUDED_REGION=0,223
9454
TARGET=0,32,AC
9455
PRIMER_COMMENT=CL is
9456
PRIMER_COMMENT=CR is
9457
=
9458
MARKER_NAME=09.RHAP68FC3.seq
9459
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGTGCATACCTTGTCATTCATGTAGAAGTTTGAAGATAACTTGTGAAGTCAGTTCTACTCTTTTACAATGTGGTTTCTAGGTTTCAAACTTAGGTTTTTAGGCTCAGCAACAAGTCCCTTTACTTCCTAAACCATCATACCAGGACCTGTAAAAGAATTGTCTTTCTTCCTGTTTAATTTAAAAAAACATTATTTTATTTCTGTN
9460
INCLUDED_REGION=9,241
9461
TARGET=9,36,AC
9462
PRIMER_COMMENT=CL is
9463
PRIMER_COMMENT=CR is
9464
=
9465
MARKER_NAME=11.RHAP68FD2.seq
9466
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNGCATGTATGCACACACACATGCACCATTATGTTTAACTTTATATATTTGTAAATATTGGTTATATCCTGTGAAGAACGTTTATTGCTAAACACTAAAACATAGAAAAAACAAATGAAGTTTGTTTAGATAATTAAGATGAGAAACTCTTTTGGGTAAGATAGGGTGATGGATTCAGGTAAAATGGGAAGCAGGAGTAATCTGG
9467
INCLUDED_REGION=0,231
9468
TARGET=0,28,AC
9469
PRIMER_COMMENT=CL is
9470
PRIMER_COMMENT=CR is
9471
=
9472
MARKER_NAME=19.RHAP68FG1.seq
9473
SEQUENCE=NNNNNNATNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATAATAATCTGANTGTGTGTGTGAATCNTCTGATGATAATCTANTGNAGATAGTGATGATGTANTACTGAAGATGTGTNATATANACTGATA
9474
INCLUDED_REGION=6,257
9475
TARGET=10,102,TG
9476
TARGET=118,52,TG
9477
PRIMER_COMMENT=CL is
9478
PRIMER_COMMENT=CR is
9479
=
9480
MARKER_NAME=20.RHAP68FG2.seq
9481
SEQUENCE=NNNNNNNNNNNNNNNNATCTGGTTCCAATGGATACCCATAATGCAAAATATGCCNNNNNNNNNNNNNCCTGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGGGGGCTCTAGGGGATCTTGCCTTTTAAAGTCAAGAGTTTAGTTGGGGGATTCAGTCTTCACTCACCAGAGGACACCATACAAATAAACTCTTCAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
9482
INCLUDED_REGION=16,208
9483
TARGET=54,13,TG
9484
TARGET=72,52,GT
9485
PRIMER_COMMENT=CL is TGGTTCCAATGGATACCCATA
9486
PRIMER_COMMENT=CR is TGAAGACTGAATCCCCCAAC
9487
PRIMER_PAIR_PENALTY=1.2106
9488
PRIMER_LEFT_PENALTY=1.113753
9489
PRIMER_RIGHT_PENALTY=0.096893
9490
PRIMER_LEFT_SEQUENCE=TGGTTCCAATGGATACCCATA
9491
PRIMER_RIGHT_SEQUENCE=TGAAGACTGAATCCCCCAAC
9492
PRIMER_LEFT=19,21
9493
PRIMER_RIGHT=187,20
9494
PRIMER_LEFT_TM=59.886
9495
PRIMER_RIGHT_TM=59.903
9496
PRIMER_LEFT_GC_PERCENT=42.857
9497
PRIMER_RIGHT_GC_PERCENT=50.000
9498
PRIMER_LEFT_SELF_ANY=9.00
9499
PRIMER_RIGHT_SELF_ANY=3.00
9500
PRIMER_LEFT_SELF_END=6.00
9501
PRIMER_RIGHT_SELF_END=0.00
9502
PRIMER_LEFT_END_STABILITY=7.4000
9503
PRIMER_RIGHT_END_STABILITY=8.2000
9504
PRIMER_PAIR_COMPL_ANY=4.00
9505
PRIMER_PAIR_COMPL_END=0.00
9506
PRIMER_PRODUCT_SIZE=169
9507
PRIMER_PAIR_PENALTY_1=1.2106
9508
PRIMER_LEFT_1_PENALTY=1.113753
9509
PRIMER_RIGHT_1_PENALTY=0.096893
9510
PRIMER_LEFT_1_SEQUENCE=TGGTTCCAATGGATACCCATA
9511
PRIMER_RIGHT_1_SEQUENCE=GTGAAGACTGAATCCCCCAA
9512
PRIMER_LEFT_1=19,21
9513
PRIMER_RIGHT_1=188,20
9514
PRIMER_LEFT_1_TM=59.886
9515
PRIMER_RIGHT_1_TM=59.903
9516
PRIMER_LEFT_1_GC_PERCENT=42.857
9517
PRIMER_RIGHT_1_GC_PERCENT=50.000
9518
PRIMER_LEFT_1_SELF_ANY=9.00
9519
PRIMER_RIGHT_1_SELF_ANY=3.00
9520
PRIMER_LEFT_1_SELF_END=6.00
9521
PRIMER_RIGHT_1_SELF_END=0.00
9522
PRIMER_LEFT_1_END_STABILITY=7.4000
9523
PRIMER_RIGHT_1_END_STABILITY=10.0000
9524
PRIMER_PAIR_1_COMPL_ANY=4.00
9525
PRIMER_PAIR_1_COMPL_END=0.00
9526
PRIMER_PRODUCT_SIZE_1=170
9527
PRIMER_PAIR_PENALTY_2=1.3030
9528
PRIMER_LEFT_2_PENALTY=1.113753
9529
PRIMER_RIGHT_2_PENALTY=0.189235
9530
PRIMER_LEFT_2_SEQUENCE=TGGTTCCAATGGATACCCATA
9531
PRIMER_RIGHT_2_SEQUENCE=AAGGCAAGATCCCCTAGAGC
9532
PRIMER_LEFT_2=19,21
9533
PRIMER_RIGHT_2=150,20
9534
PRIMER_LEFT_2_TM=59.886
9535
PRIMER_RIGHT_2_TM=59.811
9536
PRIMER_LEFT_2_GC_PERCENT=42.857
9537
PRIMER_RIGHT_2_GC_PERCENT=55.000
9538
PRIMER_LEFT_2_SELF_ANY=9.00
9539
PRIMER_RIGHT_2_SELF_ANY=4.00
9540
PRIMER_LEFT_2_SELF_END=6.00
9541
PRIMER_RIGHT_2_SELF_END=2.00
9542
PRIMER_LEFT_2_END_STABILITY=7.4000
9543
PRIMER_RIGHT_2_END_STABILITY=7.9000
9544
PRIMER_PAIR_2_COMPL_ANY=4.00
9545
PRIMER_PAIR_2_COMPL_END=0.00
9546
PRIMER_PRODUCT_SIZE_2=132
9547
PRIMER_PAIR_PENALTY_3=1.5060
9548
PRIMER_LEFT_3_PENALTY=1.409123
9549
PRIMER_RIGHT_3_PENALTY=0.096893
9550
PRIMER_LEFT_3_SEQUENCE=CCAATGGATACCCATAATGCA
9551
PRIMER_RIGHT_3_SEQUENCE=TGAAGACTGAATCCCCCAAC
9552
PRIMER_LEFT_3=24,21
9553
PRIMER_RIGHT_3=187,20
9554
PRIMER_LEFT_3_TM=60.409
9555
PRIMER_RIGHT_3_TM=59.903
9556
PRIMER_LEFT_3_GC_PERCENT=42.857
9557
PRIMER_RIGHT_3_GC_PERCENT=50.000
9558
PRIMER_LEFT_3_SELF_ANY=8.00
9559
PRIMER_RIGHT_3_SELF_ANY=3.00
9560
PRIMER_LEFT_3_SELF_END=4.00
9561
PRIMER_RIGHT_3_SELF_END=0.00
9562
PRIMER_LEFT_3_END_STABILITY=8.4000
9563
PRIMER_RIGHT_3_END_STABILITY=8.2000
9564
PRIMER_PAIR_3_COMPL_ANY=4.00
9565
PRIMER_PAIR_3_COMPL_END=0.00
9566
PRIMER_PRODUCT_SIZE_3=164
9567
PRIMER_PAIR_PENALTY_4=1.5060
9568
PRIMER_LEFT_4_PENALTY=1.409123
9569
PRIMER_RIGHT_4_PENALTY=0.096893
9570
PRIMER_LEFT_4_SEQUENCE=CCAATGGATACCCATAATGCA
9571
PRIMER_RIGHT_4_SEQUENCE=GTGAAGACTGAATCCCCCAA
9572
PRIMER_LEFT_4=24,21
9573
PRIMER_RIGHT_4=188,20
9574
PRIMER_LEFT_4_TM=60.409
9575
PRIMER_RIGHT_4_TM=59.903
9576
PRIMER_LEFT_4_GC_PERCENT=42.857
9577
PRIMER_RIGHT_4_GC_PERCENT=50.000
9578
PRIMER_LEFT_4_SELF_ANY=8.00
9579
PRIMER_RIGHT_4_SELF_ANY=3.00
9580
PRIMER_LEFT_4_SELF_END=4.00
9581
PRIMER_RIGHT_4_SELF_END=0.00
9582
PRIMER_LEFT_4_END_STABILITY=8.4000
9583
PRIMER_RIGHT_4_END_STABILITY=10.0000
9584
PRIMER_PAIR_4_COMPL_ANY=4.00
9585
PRIMER_PAIR_4_COMPL_END=0.00
9586
PRIMER_PRODUCT_SIZE_4=165
9587
=
9588
MARKER_NAME=25.RHAP68FA4.seq
9589
SEQUENCE=NNNNNNNNACGAANNNNNNNNNNNNNNNNNNNNGATGATGANNNNNNNNNNNNNNNNNNNNCATGCTCACTTCTGGGGGCTCAGGATGTCATTCCTTAGGCAATGATCATCTATGNGNTTTTGTTTTTGTTTGATAACCATGTATTCTTACCTAGACCTGTATCCCATGAAAGACATCNTGAGAGAGATCTACAAAAATGTCAACATACATATTACAAAACATAGATAGGNACTCATGAGAAATATATAAANCATTGATAGACACCATACAAATAGACTATAGACAANAGNTANAATAGAGAAGNCACAATGCGACACACACNAT
9590
INCLUDED_REGION=8,317
9591
TARGET=13,20,GT
9592
TARGET=41,20,TG
9593
PRIMER_COMMENT=CL is
9594
PRIMER_COMMENT=CR is
9595
=
9596
MARKER_NAME=27.RHAP68FA6.seq
9597
SEQUENCE=TNCCACTACTATGTCTATGGAGAACACAACAAATTGAAATAGATCAGAGCTAAGGTTCAGATTGTTCTGATCTGGAATTTGGCATCAGTGTTGAAGTTTTGAATGGCTTAGAATACCCAAGTAAATTTTTAACTAAGAAACTGTTTCAGAAGTTCTCCTAAGATCTGNNNNNNNNNNNNNNNNNNNNGATTGCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCATGATATTTCCT
9598
INCLUDED_REGION=0,247
9599
TARGET=167,20,GT
9600
TARGET=195,38,GT
9601
PRIMER_COMMENT=CL is
9602
PRIMER_COMMENT=CR is
9603
=
9604
MARKER_NAME=28.RHAP68FB4.seq
9605
SEQUENCE=NNNNNNNACACACACACACATGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGCACGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCTCCTTCACTCAGGCATCTGTTCTTTGTCAATTTTCCTCCTGAAGGGAAAAGACTTAGCACTATTACTCCTTCTGTTTTAGGAGAGATTTTTATTTTTACTT
9606
INCLUDED_REGION=7,246
9607
TARGET=24,72,CA
9608
TARGET=104,46,CA
9609
PRIMER_COMMENT=CL is
9610
PRIMER_COMMENT=CR is
9611
=
9612
MARKER_NAME=32.RHAP68FC5.seq
9613
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNTCTTTATNGCCTGTCTAAGACAGAAACAGTAAAGCTGAAACCTAAAGTCAGGGTAGTCAGGCCTAGAAAGGTGGGAAGCAGACAGTTCAGTAATTATCCATGTGCAGGGAACAGAAGAAATACATATATATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCTGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATCNCCGCAGNAGNGTGTGTGTGTGGNNNNGTGTGTGGTNATGT
9614
INCLUDED_REGION=24,244
9615
TARGET=156,29,TG
9616
TARGET=193,30,TG
9617
PRIMER_COMMENT=CL is
9618
PRIMER_COMMENT=CR is
9619
=
9620
MARKER_NAME=33.RHAP68FC6.seq
9621
SEQUENCE=NNNNNNNNNNNNNNNNACGCTTTATTAGTTCCCGAATATGTTCTATTTTCTTTTTGGTATGAGGATTCCTTCAGTTCTCTTCTGTAGTGAAGGTTTACCACTCACACACTCCTACAGCATATTTTTTATAATGGAAAGTTATTTGAAAAACAGATTTATAATTTTTATTTACCCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
9622
INCLUDED_REGION=16,243
9623
TARGET=176,46,TC
9624
TARGET=223,36,GT
9625
PRIMER_COMMENT=CL is
9626
PRIMER_COMMENT=CR is
9627
=
9628
MARKER_NAME=35.RHAP68FD5.seq
9629
SEQUENCE=NNNNNNACGGTGNCCTTGCCTGAAAATAAAAACAGTAAAGATGGAAATGTAGTTTGACAGTCTGCTTGTCCAATGAGCTTATTAGCTTAAGTTTTATCCCCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATAGATCAGGGAGAAAGATGTTCAGGGAGAGGGAGGGAGAGAGAAAGCAGGAAAAACGGTGTTCTGATACAATGCTCCTCTCCCCNTCTCTTT
9630
INCLUDED_REGION=6,250
9631
TARGET=103,59,GA
9632
PRIMER_COMMENT=CL is TGACAGTCTGCTTGTCCAATG
9633
PRIMER_COMMENT=CR is TTCTCTCTCCCTCCCTCTCC
9634
PRIMER_PAIR_PENALTY=1.2288
9635
PRIMER_LEFT_PENALTY=1.111304
9636
PRIMER_RIGHT_PENALTY=0.117486
9637
PRIMER_LEFT_SEQUENCE=TGACAGTCTGCTTGTCCAATG
9638
PRIMER_RIGHT_SEQUENCE=TTCTCTCTCCCTCCCTCTCC
9639
PRIMER_LEFT=54,21
9640
PRIMER_RIGHT=207,20
9641
PRIMER_LEFT_TM=59.889
9642
PRIMER_RIGHT_TM=59.883
9643
PRIMER_LEFT_GC_PERCENT=47.619
9644
PRIMER_RIGHT_GC_PERCENT=60.000
9645
PRIMER_LEFT_SELF_ANY=5.00
9646
PRIMER_RIGHT_SELF_ANY=0.00
9647
PRIMER_LEFT_SELF_END=3.00
9648
PRIMER_RIGHT_SELF_END=0.00
9649
PRIMER_LEFT_END_STABILITY=7.2000
9650
PRIMER_RIGHT_END_STABILITY=7.9000
9651
PRIMER_PAIR_COMPL_ANY=3.00
9652
PRIMER_PAIR_COMPL_END=1.00
9653
PRIMER_PRODUCT_SIZE=154
9654
PRIMER_PAIR_PENALTY_1=1.5200
9655
PRIMER_LEFT_1_PENALTY=1.402545
9656
PRIMER_RIGHT_1_PENALTY=0.117486
9657
PRIMER_LEFT_1_SEQUENCE=TCTGCTTGTCCAATGAGCTT
9658
PRIMER_RIGHT_1_SEQUENCE=TTCTCTCTCCCTCCCTCTCC
9659
PRIMER_LEFT_1=60,20
9660
PRIMER_RIGHT_1=207,20
9661
PRIMER_LEFT_1_TM=58.597
9662
PRIMER_RIGHT_1_TM=59.883
9663
PRIMER_LEFT_1_GC_PERCENT=45.000
9664
PRIMER_RIGHT_1_GC_PERCENT=60.000
9665
PRIMER_LEFT_1_SELF_ANY=4.00
9666
PRIMER_RIGHT_1_SELF_ANY=0.00
9667
PRIMER_LEFT_1_SELF_END=2.00
9668
PRIMER_RIGHT_1_SELF_END=0.00
9669
PRIMER_LEFT_1_END_STABILITY=8.2000
9670
PRIMER_RIGHT_1_END_STABILITY=7.9000
9671
PRIMER_PAIR_1_COMPL_ANY=3.00
9672
PRIMER_PAIR_1_COMPL_END=0.00
9673
PRIMER_PRODUCT_SIZE_1=148
9674
PRIMER_PAIR_PENALTY_2=1.5794
9675
PRIMER_LEFT_2_PENALTY=1.111304
9676
PRIMER_RIGHT_2_PENALTY=0.468086
9677
PRIMER_LEFT_2_SEQUENCE=TGACAGTCTGCTTGTCCAATG
9678
PRIMER_RIGHT_2_SEQUENCE=TGCTTTCTCTCTCCCTCCCT
9679
PRIMER_LEFT_2=54,21
9680
PRIMER_RIGHT_2=211,20
9681
PRIMER_LEFT_2_TM=59.889
9682
PRIMER_RIGHT_2_TM=60.468
9683
PRIMER_LEFT_2_GC_PERCENT=47.619
9684
PRIMER_RIGHT_2_GC_PERCENT=55.000
9685
PRIMER_LEFT_2_SELF_ANY=5.00
9686
PRIMER_RIGHT_2_SELF_ANY=2.00
9687
PRIMER_LEFT_2_SELF_END=3.00
9688
PRIMER_RIGHT_2_SELF_END=0.00
9689
PRIMER_LEFT_2_END_STABILITY=7.2000
9690
PRIMER_RIGHT_2_END_STABILITY=9.4000
9691
PRIMER_PAIR_2_COMPL_ANY=3.00
9692
PRIMER_PAIR_2_COMPL_END=1.00
9693
PRIMER_PRODUCT_SIZE_2=158
9694
PRIMER_PAIR_PENALTY_3=1.5794
9695
PRIMER_LEFT_3_PENALTY=1.111304
9696
PRIMER_RIGHT_3_PENALTY=0.468086
9697
PRIMER_LEFT_3_SEQUENCE=TGACAGTCTGCTTGTCCAATG
9698
PRIMER_RIGHT_3_SEQUENCE=CTGCTTTCTCTCTCCCTCCC
9699
PRIMER_LEFT_3=54,21
9700
PRIMER_RIGHT_3=212,20
9701
PRIMER_LEFT_3_TM=59.889
9702
PRIMER_RIGHT_3_TM=60.468
9703
PRIMER_LEFT_3_GC_PERCENT=47.619
9704
PRIMER_RIGHT_3_GC_PERCENT=60.000
9705
PRIMER_LEFT_3_SELF_ANY=5.00
9706
PRIMER_RIGHT_3_SELF_ANY=2.00
9707
PRIMER_LEFT_3_SELF_END=3.00
9708
PRIMER_RIGHT_3_SELF_END=0.00
9709
PRIMER_LEFT_3_END_STABILITY=7.2000
9710
PRIMER_RIGHT_3_END_STABILITY=9.4000
9711
PRIMER_PAIR_3_COMPL_ANY=3.00
9712
PRIMER_PAIR_3_COMPL_END=1.00
9713
PRIMER_PRODUCT_SIZE_3=159
9714
PRIMER_PAIR_PENALTY_4=1.5794
9715
PRIMER_LEFT_4_PENALTY=1.111304
9716
PRIMER_RIGHT_4_PENALTY=0.468086
9717
PRIMER_LEFT_4_SEQUENCE=TGACAGTCTGCTTGTCCAATG
9718
PRIMER_RIGHT_4_SEQUENCE=CCTGCTTTCTCTCTCCCTCC
9719
PRIMER_LEFT_4=54,21
9720
PRIMER_RIGHT_4=213,20
9721
PRIMER_LEFT_4_TM=59.889
9722
PRIMER_RIGHT_4_TM=60.468
9723
PRIMER_LEFT_4_GC_PERCENT=47.619
9724
PRIMER_RIGHT_4_GC_PERCENT=60.000
9725
PRIMER_LEFT_4_SELF_ANY=5.00
9726
PRIMER_RIGHT_4_SELF_ANY=2.00
9727
PRIMER_LEFT_4_SELF_END=3.00
9728
PRIMER_RIGHT_4_SELF_END=0.00
9729
PRIMER_LEFT_4_END_STABILITY=7.2000
9730
PRIMER_RIGHT_4_END_STABILITY=9.4000
9731
PRIMER_PAIR_4_COMPL_ANY=3.00
9732
PRIMER_PAIR_4_COMPL_END=1.00
9733
PRIMER_PRODUCT_SIZE_4=160
9734
=
9735
MARKER_NAME=36.RHAP68FD6.seq
9736
SEQUENCE=NNNNNNNNNNNNNNNACATATACATACATACATACATACATNNNNNNNNNNNNNNATACATATACACATATACATACACACACATACATATACACATATACATACATACATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAAGTTAGGGAAATAAGATTCAAGGAAGAAGCGATATTATTTTCATATCATAAAACTGGAACAGGCTTAAACCAATAGAGAAATTCAAGTGTCTCTGATTCTAAAGTTCAGTCTT
9737
INCLUDED_REGION=15,249
9738
TARGET=41,14,AC
9739
TARGET=111,38,AC
9740
PRIMER_COMMENT=CL is
9741
PRIMER_COMMENT=CR is
9742
=
9743
MARKER_NAME=41.RHAP68FF5.seq
9744
SEQUENCE=NNNNNNNNNNNNNNNNNNAATATATGCACACACATATGCGAAACCCATTTTCACTTCTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNTTTGACTACACAGGTATTTGTCAAATTAAGAATGGCAAAATAAAATGTGATGACCTGCATTTGATTAACAGTTAAGGGAACAATCTATTCTTACAGAGGACTCATATTTTTTTCCATGCTCCAATACAGAGTGGAGCATA
9745
INCLUDED_REGION=18,242
9746
TARGET=60,43,CT
9747
TARGET=104,16,TG
9748
PRIMER_COMMENT=CL is GCACACACATATGCGAAACC
9749
PRIMER_COMMENT=CR is TCAAATGCAGGTCATCACATT
9750
PRIMER_PAIR_PENALTY=2.0122
9751
PRIMER_LEFT_PENALTY=0.002648
9752
PRIMER_RIGHT_PENALTY=2.009552
9753
PRIMER_LEFT_SEQUENCE=GCACACACATATGCGAAACC
9754
PRIMER_RIGHT_SEQUENCE=AATCAAATGCAGGTCATCACA
9755
PRIMER_LEFT=25,20
9756
PRIMER_RIGHT=185,21
9757
PRIMER_LEFT_TM=60.003
9758
PRIMER_RIGHT_TM=58.990
9759
PRIMER_LEFT_GC_PERCENT=50.000
9760
PRIMER_RIGHT_GC_PERCENT=38.095
9761
PRIMER_LEFT_SELF_ANY=6.00
9762
PRIMER_RIGHT_SELF_ANY=4.00
9763
PRIMER_LEFT_SELF_END=0.00
9764
PRIMER_RIGHT_SELF_END=2.00
9765
PRIMER_LEFT_END_STABILITY=8.2000
9766
PRIMER_RIGHT_END_STABILITY=6.7000
9767
PRIMER_PAIR_COMPL_ANY=4.00
9768
PRIMER_PAIR_COMPL_END=3.00
9769
PRIMER_PRODUCT_SIZE=161
9770
PRIMER_PAIR_PENALTY_1=2.0122
9771
PRIMER_LEFT_1_PENALTY=0.002648
9772
PRIMER_RIGHT_1_PENALTY=2.009552
9773
PRIMER_LEFT_1_SEQUENCE=GCACACACATATGCGAAACC
9774
PRIMER_RIGHT_1_SEQUENCE=TCAAATGCAGGTCATCACATT
9775
PRIMER_LEFT_1=25,20
9776
PRIMER_RIGHT_1=183,21
9777
PRIMER_LEFT_1_TM=60.003
9778
PRIMER_RIGHT_1_TM=58.990
9779
PRIMER_LEFT_1_GC_PERCENT=50.000
9780
PRIMER_RIGHT_1_GC_PERCENT=38.095
9781
PRIMER_LEFT_1_SELF_ANY=6.00
9782
PRIMER_RIGHT_1_SELF_ANY=4.00
9783
PRIMER_LEFT_1_SELF_END=0.00
9784
PRIMER_RIGHT_1_SELF_END=3.00
9785
PRIMER_LEFT_1_END_STABILITY=8.2000
9786
PRIMER_RIGHT_1_END_STABILITY=6.6000
9787
PRIMER_PAIR_1_COMPL_ANY=4.00
9788
PRIMER_PAIR_1_COMPL_END=3.00
9789
PRIMER_PRODUCT_SIZE_1=159
9790
PRIMER_PAIR_PENALTY_2=2.1355
9791
PRIMER_LEFT_2_PENALTY=0.002648
9792
PRIMER_RIGHT_2_PENALTY=2.132857
9793
PRIMER_LEFT_2_SEQUENCE=GCACACACATATGCGAAACC
9794
PRIMER_RIGHT_2_SEQUENCE=CAAATGCAGGTCATCACATTTT
9795
PRIMER_LEFT_2=25,20
9796
PRIMER_RIGHT_2=182,22
9797
PRIMER_LEFT_2_TM=60.003
9798
PRIMER_RIGHT_2_TM=59.867
9799
PRIMER_LEFT_2_GC_PERCENT=50.000
9800
PRIMER_RIGHT_2_GC_PERCENT=36.364
9801
PRIMER_LEFT_2_SELF_ANY=6.00
9802
PRIMER_RIGHT_2_SELF_ANY=5.00
9803
PRIMER_LEFT_2_SELF_END=0.00
9804
PRIMER_RIGHT_2_SELF_END=3.00
9805
PRIMER_LEFT_2_END_STABILITY=8.2000
9806
PRIMER_RIGHT_2_END_STABILITY=7.2000
9807
PRIMER_PAIR_2_COMPL_ANY=4.00
9808
PRIMER_PAIR_2_COMPL_END=3.00
9809
PRIMER_PRODUCT_SIZE_2=158
9810
PRIMER_PAIR_PENALTY_3=2.1626
9811
PRIMER_LEFT_3_PENALTY=0.152999
9812
PRIMER_RIGHT_3_PENALTY=2.009552
9813
PRIMER_LEFT_3_SEQUENCE=ACACACATATGCGAAACCCA
9814
PRIMER_RIGHT_3_SEQUENCE=AATCAAATGCAGGTCATCACA
9815
PRIMER_LEFT_3=27,20
9816
PRIMER_RIGHT_3=185,21
9817
PRIMER_LEFT_3_TM=59.847
9818
PRIMER_RIGHT_3_TM=58.990
9819
PRIMER_LEFT_3_GC_PERCENT=45.000
9820
PRIMER_RIGHT_3_GC_PERCENT=38.095
9821
PRIMER_LEFT_3_SELF_ANY=6.00
9822
PRIMER_RIGHT_3_SELF_ANY=4.00
9823
PRIMER_LEFT_3_SELF_END=1.00
9824
PRIMER_RIGHT_3_SELF_END=2.00
9825
PRIMER_LEFT_3_END_STABILITY=9.4000
9826
PRIMER_RIGHT_3_END_STABILITY=6.7000
9827
PRIMER_PAIR_3_COMPL_ANY=4.00
9828
PRIMER_PAIR_3_COMPL_END=1.00
9829
PRIMER_PRODUCT_SIZE_3=159
9830
PRIMER_PAIR_PENALTY_4=2.1626
9831
PRIMER_LEFT_4_PENALTY=0.152999
9832
PRIMER_RIGHT_4_PENALTY=2.009552
9833
PRIMER_LEFT_4_SEQUENCE=CACACACATATGCGAAACCC
9834
PRIMER_RIGHT_4_SEQUENCE=AATCAAATGCAGGTCATCACA
9835
PRIMER_LEFT_4=26,20
9836
PRIMER_RIGHT_4=185,21
9837
PRIMER_LEFT_4_TM=59.847
9838
PRIMER_RIGHT_4_TM=58.990
9839
PRIMER_LEFT_4_GC_PERCENT=50.000
9840
PRIMER_RIGHT_4_GC_PERCENT=38.095
9841
PRIMER_LEFT_4_SELF_ANY=6.00
9842
PRIMER_RIGHT_4_SELF_ANY=4.00
9843
PRIMER_LEFT_4_SELF_END=0.00
9844
PRIMER_RIGHT_4_SELF_END=2.00
9845
PRIMER_LEFT_4_END_STABILITY=9.4000
9846
PRIMER_RIGHT_4_END_STABILITY=6.7000
9847
PRIMER_PAIR_4_COMPL_ANY=4.00
9848
PRIMER_PAIR_4_COMPL_END=2.00
9849
PRIMER_PRODUCT_SIZE_4=160
9850
=
9851
MARKER_NAME=42.RHAP68FF6.seq
9852
SEQUENCE=NNNNNNNNNNNNNNNNACTCANGATGCTGATCCAATTATCTACAACCTTATAAACATATTTGATAAGTATTCTGAAAAGGCAATCATGAATACACAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATGTTCCACTGAAAATTTGGAATGAGTTTTTCATTCCTCAAGTTCTTTAGAAATAAAAAATTCTGCTAAGAAAATCATTAGCTCAAAAGACAATGAAAGTAACAAATTATTTAGCTCTTTCAAG
9853
INCLUDED_REGION=16,245
9854
TARGET=98,38,TG
9855
PRIMER_COMMENT=CL is TGCTGATCCAATTATCTACAACC
9856
PRIMER_COMMENT=CR is TCCAAATTTTCAGTGGAACATG
9857
PRIMER_PAIR_PENALTY=7.0107
9858
PRIMER_LEFT_PENALTY=4.849609
9859
PRIMER_RIGHT_PENALTY=2.161083
9860
PRIMER_LEFT_SEQUENCE=TGCTGATCCAATTATCTACAACC
9861
PRIMER_RIGHT_SEQUENCE=TCCAAATTTTCAGTGGAACATG
9862
PRIMER_LEFT=24,23
9863
PRIMER_RIGHT=157,22
9864
PRIMER_LEFT_TM=58.150
9865
PRIMER_RIGHT_TM=59.839
9866
PRIMER_LEFT_GC_PERCENT=39.130
9867
PRIMER_RIGHT_GC_PERCENT=36.364
9868
PRIMER_LEFT_SELF_ANY=4.00
9869
PRIMER_RIGHT_SELF_ANY=6.00
9870
PRIMER_LEFT_SELF_END=0.00
9871
PRIMER_RIGHT_SELF_END=4.00
9872
PRIMER_LEFT_END_STABILITY=8.2000
9873
PRIMER_RIGHT_END_STABILITY=6.6000
9874
PRIMER_PAIR_COMPL_ANY=5.00
9875
PRIMER_PAIR_COMPL_END=1.00
9876
PRIMER_PRODUCT_SIZE=134
9877
PRIMER_PAIR_PENALTY_1=7.0790
9878
PRIMER_LEFT_1_PENALTY=4.917956
9879
PRIMER_RIGHT_1_PENALTY=2.161083
9880
PRIMER_LEFT_1_SEQUENCE=TGCTGATCCAATTATCTACAACCT
9881
PRIMER_RIGHT_1_SEQUENCE=TCCAAATTTTCAGTGGAACATG
9882
PRIMER_LEFT_1=24,24
9883
PRIMER_RIGHT_1=157,22
9884
PRIMER_LEFT_1_TM=59.082
9885
PRIMER_RIGHT_1_TM=59.839
9886
PRIMER_LEFT_1_GC_PERCENT=37.500
9887
PRIMER_RIGHT_1_GC_PERCENT=36.364
9888
PRIMER_LEFT_1_SELF_ANY=4.00
9889
PRIMER_RIGHT_1_SELF_ANY=6.00
9890
PRIMER_LEFT_1_SELF_END=0.00
9891
PRIMER_RIGHT_1_SELF_END=4.00
9892
PRIMER_LEFT_1_END_STABILITY=7.9000
9893
PRIMER_RIGHT_1_END_STABILITY=6.6000
9894
PRIMER_PAIR_1_COMPL_ANY=5.00
9895
PRIMER_PAIR_1_COMPL_END=1.00
9896
PRIMER_PRODUCT_SIZE_1=134
9897
PRIMER_PAIR_PENALTY_2=7.2752
9898
PRIMER_LEFT_2_PENALTY=5.114154
9899
PRIMER_RIGHT_2_PENALTY=2.161083
9900
PRIMER_LEFT_2_SEQUENCE=GATGCTGATCCAATTATCTACAACC
9901
PRIMER_RIGHT_2_SEQUENCE=TCCAAATTTTCAGTGGAACATG
9902
PRIMER_LEFT_2=22,25
9903
PRIMER_RIGHT_2=157,22
9904
PRIMER_LEFT_2_TM=60.114
9905
PRIMER_RIGHT_2_TM=59.839
9906
PRIMER_LEFT_2_GC_PERCENT=40.000
9907
PRIMER_RIGHT_2_GC_PERCENT=36.364
9908
PRIMER_LEFT_2_SELF_ANY=4.00
9909
PRIMER_RIGHT_2_SELF_ANY=6.00
9910
PRIMER_LEFT_2_SELF_END=0.00
9911
PRIMER_RIGHT_2_SELF_END=4.00
9912
PRIMER_LEFT_2_END_STABILITY=8.2000
9913
PRIMER_RIGHT_2_END_STABILITY=6.6000
9914
PRIMER_PAIR_2_COMPL_ANY=5.00
9915
PRIMER_PAIR_2_COMPL_END=1.00
9916
PRIMER_PRODUCT_SIZE_2=136
9917
PRIMER_PAIR_PENALTY_3=7.4183
9918
PRIMER_LEFT_3_PENALTY=5.257212
9919
PRIMER_RIGHT_3_PENALTY=2.161083
9920
PRIMER_LEFT_3_SEQUENCE=TGCTGATCCAATTATCTACAACCTT
9921
PRIMER_RIGHT_3_SEQUENCE=TCCAAATTTTCAGTGGAACATG
9922
PRIMER_LEFT_3=24,25
9923
PRIMER_RIGHT_3=157,22
9924
PRIMER_LEFT_3_TM=60.257
9925
PRIMER_RIGHT_3_TM=59.839
9926
PRIMER_LEFT_3_GC_PERCENT=36.000
9927
PRIMER_RIGHT_3_GC_PERCENT=36.364
9928
PRIMER_LEFT_3_SELF_ANY=4.00
9929
PRIMER_RIGHT_3_SELF_ANY=6.00
9930
PRIMER_LEFT_3_SELF_END=2.00
9931
PRIMER_RIGHT_3_SELF_END=4.00
9932
PRIMER_LEFT_3_END_STABILITY=7.9000
9933
PRIMER_RIGHT_3_END_STABILITY=6.6000
9934
PRIMER_PAIR_3_COMPL_ANY=5.00
9935
PRIMER_PAIR_3_COMPL_END=1.00
9936
PRIMER_PRODUCT_SIZE_3=134
9937
PRIMER_PAIR_PENALTY_4=7.4207
9938
PRIMER_LEFT_4_PENALTY=4.849609
9939
PRIMER_RIGHT_4_PENALTY=2.571122
9940
PRIMER_LEFT_4_SEQUENCE=TGCTGATCCAATTATCTACAACC
9941
PRIMER_RIGHT_4_SEQUENCE=TCATTCCAAATTTTCAGTGGAA
9942
PRIMER_LEFT_4=24,23
9943
PRIMER_RIGHT_4=161,22
9944
PRIMER_LEFT_4_TM=58.150
9945
PRIMER_RIGHT_4_TM=59.429
9946
PRIMER_LEFT_4_GC_PERCENT=39.130
9947
PRIMER_RIGHT_4_GC_PERCENT=31.818
9948
PRIMER_LEFT_4_SELF_ANY=4.00
9949
PRIMER_RIGHT_4_SELF_ANY=6.00
9950
PRIMER_LEFT_4_SELF_END=0.00
9951
PRIMER_RIGHT_4_SELF_END=5.00
9952
PRIMER_LEFT_4_END_STABILITY=8.2000
9953
PRIMER_RIGHT_4_END_STABILITY=8.5000
9954
PRIMER_PAIR_4_COMPL_ANY=4.00
9955
PRIMER_PAIR_4_COMPL_END=1.00
9956
PRIMER_PRODUCT_SIZE_4=138
9957
=
9958
MARKER_NAME=45.RHAP68FG6.seq
9959
SEQUENCE=NNNNNNNACANNATACANGTTGCTACTAGGTCCTATGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGTATATGTATATATGTTCAANNNNNNNNNNNNNNNNNNNNNNNCTTTTAGCTTATTTTTATGTGACTCCTGGCTATGAAAAGGAGCGGGTGCTGACACTGGTTGTAGCTCTTGTGACACTTTTCCTCCTGTTGGGGTT
9960
INCLUDED_REGION=7,240
9961
TARGET=39,67,TG
9962
TARGET=129,23,AT
9963
PRIMER_COMMENT=CL is
9964
PRIMER_COMMENT=CR is
9965
=
9966
MARKER_NAME=48.RHAP68FH6.seq
9967
SEQUENCE=NNNNNNNNNNNNNNNNNTTATGTATTTAGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAACAATCATGTGCTGTGTTATAACAACAAAGAGTAAATAAAACGGCCATGAATTTGAGAGAGAGCATGGAGGGTTTATGGGAGGGAGGAAAGGAAAAGCTCATATGATATAATTTTAGAAAGAGAAAAAAAATCAGGAAACACATGTATTCAGTTTTCCCAAAGACTCTGAAGCGTAACACTAGTTTATGCAGCAGAATG
9968
INCLUDED_REGION=17,246
9969
TARGET=31,32,GT
9970
PRIMER_COMMENT=CL is
9971
PRIMER_COMMENT=CR is
9972
=
9973
MARKER_NAME=01.RHAP69FA7.seq
9974
SEQUENCE=TGNGNTACGTTNCNCTGNTTTGGCCGTTTTTTGCCNATCCGGNGTCACACTGTTTGAGCCACGGATATCCCAGTCAGCTGTTTCCTACCTTTCCACATAAGGGAGAAATCTTAGTCCTTATGTGGGGCCAAGAATACATGGGGTGGGGGTGGGGGCATAAACAGGTTTTGCTTCTTGCGCTATATGGAATGGAAAGAACCAGACTACAGAGCGTGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCCTANACAAATTGGGGAGGGGGGTNTAGAAACCCAGGGCAAGCAGATCCCAGGAGCACCGAGNAGGC
9975
INCLUDED_REGION=0,326
9976
TARGET=217,40,CA
9977
PRIMER_COMMENT=CL is AGGTTTTGCTTCTTGCGCTA
9978
PRIMER_COMMENT=CR is GTGCTCCTGGGATCTGCTT
9979
PRIMER_PAIR_PENALTY=1.5170
9980
PRIMER_LEFT_PENALTY=0.151911
9981
PRIMER_RIGHT_PENALTY=1.365052
9982
PRIMER_LEFT_SEQUENCE=AGGTTTTGCTTCTTGCGCTA
9983
PRIMER_RIGHT_SEQUENCE=GTGCTCCTGGGATCTGCTT
9984
PRIMER_LEFT=162,20
9985
PRIMER_RIGHT=316,19
9986
PRIMER_LEFT_TM=60.152
9987
PRIMER_RIGHT_TM=60.365
9988
PRIMER_LEFT_GC_PERCENT=45.000
9989
PRIMER_RIGHT_GC_PERCENT=57.895
9990
PRIMER_LEFT_SELF_ANY=4.00
9991
PRIMER_RIGHT_SELF_ANY=4.00
9992
PRIMER_LEFT_SELF_END=4.00
9993
PRIMER_RIGHT_SELF_END=0.00
9994
PRIMER_LEFT_END_STABILITY=9.2000
9995
PRIMER_RIGHT_END_STABILITY=8.5000
9996
PRIMER_PAIR_COMPL_ANY=3.00
9997
PRIMER_PAIR_COMPL_END=1.00
9998
PRIMER_PRODUCT_SIZE=155
9999
PRIMER_PAIR_PENALTY_1=1.7848
10000
PRIMER_LEFT_1_PENALTY=0.419726
10001
PRIMER_RIGHT_1_PENALTY=1.365052
10002
PRIMER_LEFT_1_SEQUENCE=AACAGGTTTTGCTTCTTGCG
10003
PRIMER_RIGHT_1_SEQUENCE=GTGCTCCTGGGATCTGCTT
10004
PRIMER_LEFT_1=159,20
10005
PRIMER_RIGHT_1=316,19
10006
PRIMER_LEFT_1_TM=60.420
10007
PRIMER_RIGHT_1_TM=60.365
10008
PRIMER_LEFT_1_GC_PERCENT=45.000
10009
PRIMER_RIGHT_1_GC_PERCENT=57.895
10010
PRIMER_LEFT_1_SELF_ANY=4.00
10011
PRIMER_RIGHT_1_SELF_ANY=4.00
10012
PRIMER_LEFT_1_SELF_END=2.00
10013
PRIMER_RIGHT_1_SELF_END=0.00
10014
PRIMER_LEFT_1_END_STABILITY=10.5000
10015
PRIMER_RIGHT_1_END_STABILITY=8.5000
10016
PRIMER_PAIR_1_COMPL_ANY=4.00
10017
PRIMER_PAIR_1_COMPL_END=0.00
10018
PRIMER_PRODUCT_SIZE_1=158
10019
PRIMER_PAIR_PENALTY_2=1.7982
10020
PRIMER_LEFT_2_PENALTY=0.433114
10021
PRIMER_RIGHT_2_PENALTY=1.365052
10022
PRIMER_LEFT_2_SEQUENCE=TGCTTCTTGCGCTATATGGA
10023
PRIMER_RIGHT_2_SEQUENCE=GTGCTCCTGGGATCTGCTT
10024
PRIMER_LEFT_2=168,20
10025
PRIMER_RIGHT_2=316,19
10026
PRIMER_LEFT_2_TM=59.567
10027
PRIMER_RIGHT_2_TM=60.365
10028
PRIMER_LEFT_2_GC_PERCENT=45.000
10029
PRIMER_RIGHT_2_GC_PERCENT=57.895
10030
PRIMER_LEFT_2_SELF_ANY=4.00
10031
PRIMER_RIGHT_2_SELF_ANY=4.00
10032
PRIMER_LEFT_2_SELF_END=4.00
10033
PRIMER_RIGHT_2_SELF_END=0.00
10034
PRIMER_LEFT_2_END_STABILITY=8.1000
10035
PRIMER_RIGHT_2_END_STABILITY=8.5000
10036
PRIMER_PAIR_2_COMPL_ANY=3.00
10037
PRIMER_PAIR_2_COMPL_END=1.00
10038
PRIMER_PRODUCT_SIZE_2=149
10039
PRIMER_PAIR_PENALTY_3=1.8639
10040
PRIMER_LEFT_3_PENALTY=0.498888
10041
PRIMER_RIGHT_3_PENALTY=1.365052
10042
PRIMER_LEFT_3_SEQUENCE=GGTTTTGCTTCTTGCGCTAT
10043
PRIMER_RIGHT_3_SEQUENCE=GTGCTCCTGGGATCTGCTT
10044
PRIMER_LEFT_3=163,20
10045
PRIMER_RIGHT_3=316,19
10046
PRIMER_LEFT_3_TM=59.501
10047
PRIMER_RIGHT_3_TM=60.365
10048
PRIMER_LEFT_3_GC_PERCENT=45.000
10049
PRIMER_RIGHT_3_GC_PERCENT=57.895
10050
PRIMER_LEFT_3_SELF_ANY=4.00
10051
PRIMER_RIGHT_3_SELF_ANY=4.00
10052
PRIMER_LEFT_3_SELF_END=2.00
10053
PRIMER_RIGHT_3_SELF_END=0.00
10054
PRIMER_LEFT_3_END_STABILITY=7.1000
10055
PRIMER_RIGHT_3_END_STABILITY=8.5000
10056
PRIMER_PAIR_3_COMPL_ANY=2.00
10057
PRIMER_PAIR_3_COMPL_END=0.00
10058
PRIMER_PRODUCT_SIZE_3=154
10059
PRIMER_PAIR_PENALTY_4=2.2399
10060
PRIMER_LEFT_4_PENALTY=0.874885
10061
PRIMER_RIGHT_4_PENALTY=1.365052
10062
PRIMER_LEFT_4_SEQUENCE=TGCGCTATATGGAATGGAAA
10063
PRIMER_RIGHT_4_SEQUENCE=GTGCTCCTGGGATCTGCTT
10064
PRIMER_LEFT_4=175,20
10065
PRIMER_RIGHT_4=316,19
10066
PRIMER_LEFT_4_TM=59.125
10067
PRIMER_RIGHT_4_TM=60.365
10068
PRIMER_LEFT_4_GC_PERCENT=40.000
10069
PRIMER_RIGHT_4_GC_PERCENT=57.895
10070
PRIMER_LEFT_4_SELF_ANY=4.00
10071
PRIMER_RIGHT_4_SELF_ANY=4.00
10072
PRIMER_LEFT_4_SELF_END=0.00
10073
PRIMER_RIGHT_4_SELF_END=0.00
10074
PRIMER_LEFT_4_END_STABILITY=8.5000
10075
PRIMER_RIGHT_4_END_STABILITY=8.5000
10076
PRIMER_PAIR_4_COMPL_ANY=3.00
10077
PRIMER_PAIR_4_COMPL_END=2.00
10078
PRIMER_PRODUCT_SIZE_4=142
10079
=
10080
MARKER_NAME=03.RHAP69FA9.seq
10081
SEQUENCE=NNNNNNNNNNNNNNNNNNNNACGTTCTCCTGTAGGACAAAGACTTTCTGTGGTTACGACGGATATTTCTCAGACTTTATCATTAAAAAGAACTGACATACAGCTGCGGCTGTAGTTCACATGTAGGATATTTGCTGAGCATGGATGAAACTCTGGGTTTGAGTCCCACCATCAGAAAAATGTGCTTCCCATTTATATGGAGAGTTATTAAATTTGTTCTCACAGTTTGATCCATTTCTCCTCAATATAAAGATCTATGAAGGAATAAGAAGGGAGAGTTCTGCTTTGATGTTAATTAGCAAGAAAGACGACATAGCAAGAAACAGAGCTGAGTCCAGATTCCAGCACATGTGCGCTNNNNNNNNNNNNNN
10082
INCLUDED_REGION=20,350
10083
TARGET=356,14,CA
10084
PRIMER_COMMENT=CL is
10085
PRIMER_COMMENT=CR is
10086
=
10087
MARKER_NAME=04.RHAP69FB1.seq
10088
SEQUENCE=CNGNATTATNTCCANAAANAAATTATTTNATTGCAACANAAAACATTACATGTCAGCCTTAAAAAGCAATTAAATCAAAAAAACAAAAATACCAATTTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTTCATCTCAAGACTCTGTTTCTGTTGTGGGGCCTGTGGGGTCTGGAGCTCCTTTGAAACGGAGACTTGGAAATTGCTCACCTGGCTGAGCTTTGGCCCCTCAGAGGTGGGACTTTCTTTACTAACAATAGGAAGCTGGAGAAGCTGTCAAGTGCGAATGGAGGAGACCTGCTCAGAAAGGGAGGGGAGCTGTGAGAATTTGGTTTCTCTCAGTATTGGGGTGGGGGTGGGGTCTGGATTTACTTGTGCCTAAGATTCCTTTAAAACTCTGACCCGATGCA
10089
INCLUDED_REGION=0,435
10090
TARGET=100,52,AC
10091
PRIMER_COMMENT=CL is CATGTCAGCCTTAAAAAGCAA
10092
PRIMER_COMMENT=CR is TCTCCGTTTCAAAGGAGCTC
10093
PRIMER_PAIR_PENALTY=3.3396
10094
PRIMER_LEFT_PENALTY=2.889802
10095
PRIMER_RIGHT_PENALTY=0.449829
10096
PRIMER_LEFT_SEQUENCE=CATGTCAGCCTTAAAAAGCAA
10097
PRIMER_RIGHT_SEQUENCE=TCTCCGTTTCAAAGGAGCTC
10098
PRIMER_LEFT=48,21
10099
PRIMER_RIGHT=218,20
10100
PRIMER_LEFT_TM=58.110
10101
PRIMER_RIGHT_TM=59.550
10102
PRIMER_LEFT_GC_PERCENT=38.095
10103
PRIMER_RIGHT_GC_PERCENT=50.000
10104
PRIMER_LEFT_SELF_ANY=4.00
10105
PRIMER_RIGHT_SELF_ANY=9.00
10106
PRIMER_LEFT_SELF_END=0.00
10107
PRIMER_RIGHT_SELF_END=6.00
10108
PRIMER_LEFT_END_STABILITY=8.5000
10109
PRIMER_RIGHT_END_STABILITY=7.9000
10110
PRIMER_PAIR_COMPL_ANY=5.00
10111
PRIMER_PAIR_COMPL_END=0.00
10112
PRIMER_PRODUCT_SIZE=171
10113
PRIMER_PAIR_PENALTY_1=3.3973
10114
PRIMER_LEFT_1_PENALTY=2.889802
10115
PRIMER_RIGHT_1_PENALTY=0.507521
10116
PRIMER_LEFT_1_SEQUENCE=CATGTCAGCCTTAAAAAGCAA
10117
PRIMER_RIGHT_1_SEQUENCE=CGTTTCAAAGGAGCTCCAGA
10118
PRIMER_LEFT_1=48,21
10119
PRIMER_RIGHT_1=214,20
10120
PRIMER_LEFT_1_TM=58.110
10121
PRIMER_RIGHT_1_TM=60.508
10122
PRIMER_LEFT_1_GC_PERCENT=38.095
10123
PRIMER_RIGHT_1_GC_PERCENT=50.000
10124
PRIMER_LEFT_1_SELF_ANY=4.00
10125
PRIMER_RIGHT_1_SELF_ANY=8.00
10126
PRIMER_LEFT_1_SELF_END=0.00
10127
PRIMER_RIGHT_1_SELF_END=2.00
10128
PRIMER_LEFT_1_END_STABILITY=8.5000
10129
PRIMER_RIGHT_1_END_STABILITY=8.2000
10130
PRIMER_PAIR_1_COMPL_ANY=5.00
10131
PRIMER_PAIR_1_COMPL_END=0.00
10132
PRIMER_PRODUCT_SIZE_1=167
10133
PRIMER_PAIR_PENALTY_2=3.4845
10134
PRIMER_LEFT_2_PENALTY=2.889802
10135
PRIMER_RIGHT_2_PENALTY=0.594713
10136
PRIMER_LEFT_2_SEQUENCE=CATGTCAGCCTTAAAAAGCAA
10137
PRIMER_RIGHT_2_SEQUENCE=TTTCAAAGGAGCTCCAGACC
10138
PRIMER_LEFT_2=48,21
10139
PRIMER_RIGHT_2=212,20
10140
PRIMER_LEFT_2_TM=58.110
10141
PRIMER_RIGHT_2_TM=59.405
10142
PRIMER_LEFT_2_GC_PERCENT=38.095
10143
PRIMER_RIGHT_2_GC_PERCENT=50.000
10144
PRIMER_LEFT_2_SELF_ANY=4.00
10145
PRIMER_RIGHT_2_SELF_ANY=8.00
10146
PRIMER_LEFT_2_SELF_END=0.00
10147
PRIMER_RIGHT_2_SELF_END=2.00
10148
PRIMER_LEFT_2_END_STABILITY=8.5000
10149
PRIMER_RIGHT_2_END_STABILITY=7.6000
10150
PRIMER_PAIR_2_COMPL_ANY=5.00
10151
PRIMER_PAIR_2_COMPL_END=0.00
10152
PRIMER_PRODUCT_SIZE_2=165
10153
PRIMER_PAIR_PENALTY_3=3.5048
10154
PRIMER_LEFT_3_PENALTY=3.055020
10155
PRIMER_RIGHT_3_PENALTY=0.449829
10156
PRIMER_LEFT_3_SEQUENCE=ACATGTCAGCCTTAAAAAGCAA
10157
PRIMER_RIGHT_3_SEQUENCE=TCTCCGTTTCAAAGGAGCTC
10158
PRIMER_LEFT_3=47,22
10159
PRIMER_RIGHT_3=218,20
10160
PRIMER_LEFT_3_TM=58.945
10161
PRIMER_RIGHT_3_TM=59.550
10162
PRIMER_LEFT_3_GC_PERCENT=36.364
10163
PRIMER_RIGHT_3_GC_PERCENT=50.000
10164
PRIMER_LEFT_3_SELF_ANY=6.00
10165
PRIMER_RIGHT_3_SELF_ANY=9.00
10166
PRIMER_LEFT_3_SELF_END=0.00
10167
PRIMER_RIGHT_3_SELF_END=6.00
10168
PRIMER_LEFT_3_END_STABILITY=8.5000
10169
PRIMER_RIGHT_3_END_STABILITY=7.9000
10170
PRIMER_PAIR_3_COMPL_ANY=5.00
10171
PRIMER_PAIR_3_COMPL_END=0.00
10172
PRIMER_PRODUCT_SIZE_3=172
10173
PRIMER_PAIR_PENALTY_4=3.5625
10174
PRIMER_LEFT_4_PENALTY=3.055020
10175
PRIMER_RIGHT_4_PENALTY=0.507521
10176
PRIMER_LEFT_4_SEQUENCE=ACATGTCAGCCTTAAAAAGCAA
10177
PRIMER_RIGHT_4_SEQUENCE=CGTTTCAAAGGAGCTCCAGA
10178
PRIMER_LEFT_4=47,22
10179
PRIMER_RIGHT_4=214,20
10180
PRIMER_LEFT_4_TM=58.945
10181
PRIMER_RIGHT_4_TM=60.508
10182
PRIMER_LEFT_4_GC_PERCENT=36.364
10183
PRIMER_RIGHT_4_GC_PERCENT=50.000
10184
PRIMER_LEFT_4_SELF_ANY=6.00
10185
PRIMER_RIGHT_4_SELF_ANY=8.00
10186
PRIMER_LEFT_4_SELF_END=0.00
10187
PRIMER_RIGHT_4_SELF_END=2.00
10188
PRIMER_LEFT_4_END_STABILITY=8.5000
10189
PRIMER_RIGHT_4_END_STABILITY=8.2000
10190
PRIMER_PAIR_4_COMPL_ANY=5.00
10191
PRIMER_PAIR_4_COMPL_END=0.00
10192
PRIMER_PRODUCT_SIZE_4=168
10193
=
10194
MARKER_NAME=09.RHAP69FC9.seq
10195
SEQUENCE=NNNNCACAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGGTTAGTTAAGAAGACAGTTAAAGATGGATTGAAAGTAAGCATTGTGGTTTGTGGTTAGACCTTGTCAAACTGTTATCCAGATAAGTCATAAAATATTTCAATGGTTCGTTTGAACCGATATAAATTCTTTCTAAAGAAATACTATAGTATTGCATTAGCAATATGGCACTGTGATAAATTAGAAAAGAGATTAAGTGTTCACACCTCGGATTGTTGTTTTCTCACTAAGCAATTATGTTAATTCAGAACTATGCAAAGCAAGGAGGAAAGAAAATAAGAAGCCACAAAGA
10196
INCLUDED_REGION=4,350
10197
TARGET=9,52,TG
10198
PRIMER_COMMENT=CL is
10199
PRIMER_COMMENT=CR is
10200
=
10201
MARKER_NAME=10.RHAP69FD1.seq
10202
SEQUENCE=NNNNNNNNNNNNNNNNACTTTTTAAAAAACCAAAAATCAGGGGAGATGGCTCACACAGCAAAGGGCCAGATGTGTGAGCTTAAGGTTCGAGGCACTCCTGTCCAGTGTCTTCTGAGATTATTACAGTTCTTGGGAAGTAAAGACATGAAGATGACTGGACCAAACTTGTCAGCCCACTCTAGCAAAATAAGACTGCTAGGTTCAATGAAAAACCCTATCTAAAAAAATAAGGAAAGTGATGAAGAGTGAAGACACCTGATGTTAGCTTTTGACCTCCAAGTCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGCATACATACATGCACACACATGTGCATGCACATACAAACAATCATGCACACAGACATAAGCTCTAGAAATTACCATCAGTAATGCAACAGACTTTTTAAAGACTGGTATTCTAATAGTAAGTNNNNNNNNN
10203
INCLUDED_REGION=16,443
10204
TARGET=284,50,AC
10205
PRIMER_COMMENT=CL is TGCTAGGTTCAATGAAAAACCC
10206
PRIMER_COMMENT=CR is ATGCACATGTGTGTGCATGT
10207
PRIMER_PAIR_PENALTY=2.9019
10208
PRIMER_LEFT_PENALTY=2.343977
10209
PRIMER_RIGHT_PENALTY=0.557889
10210
PRIMER_LEFT_SEQUENCE=TGCTAGGTTCAATGAAAAACCC
10211
PRIMER_RIGHT_SEQUENCE=ATGCACATGTGTGTGCATGT
10212
PRIMER_LEFT=193,22
10213
PRIMER_RIGHT=363,20
10214
PRIMER_LEFT_TM=60.344
10215
PRIMER_RIGHT_TM=59.442
10216
PRIMER_LEFT_GC_PERCENT=40.909
10217
PRIMER_RIGHT_GC_PERCENT=45.000
10218
PRIMER_LEFT_SELF_ANY=7.00
10219
PRIMER_RIGHT_SELF_ANY=10.00
10220
PRIMER_LEFT_SELF_END=0.00
10221
PRIMER_RIGHT_SELF_END=4.00
10222
PRIMER_LEFT_END_STABILITY=9.4000
10223
PRIMER_RIGHT_END_STABILITY=6.6000
10224
PRIMER_PAIR_COMPL_ANY=3.00
10225
PRIMER_PAIR_COMPL_END=1.00
10226
PRIMER_PRODUCT_SIZE=171
10227
PRIMER_PAIR_PENALTY_1=3.2301
10228
PRIMER_LEFT_1_PENALTY=2.343977
10229
PRIMER_RIGHT_1_PENALTY=0.886116
10230
PRIMER_LEFT_1_SEQUENCE=TGCTAGGTTCAATGAAAAACCC
10231
PRIMER_RIGHT_1_SEQUENCE=TGCACATGTGTGTGCATGTA
10232
PRIMER_LEFT_1=193,22
10233
PRIMER_RIGHT_1=362,20
10234
PRIMER_LEFT_1_TM=60.344
10235
PRIMER_RIGHT_1_TM=59.114
10236
PRIMER_LEFT_1_GC_PERCENT=40.909
10237
PRIMER_RIGHT_1_GC_PERCENT=45.000
10238
PRIMER_LEFT_1_SELF_ANY=7.00
10239
PRIMER_RIGHT_1_SELF_ANY=8.00
10240
PRIMER_LEFT_1_SELF_END=0.00
10241
PRIMER_RIGHT_1_SELF_END=4.00
10242
PRIMER_LEFT_1_END_STABILITY=9.4000
10243
PRIMER_RIGHT_1_END_STABILITY=5.6000
10244
PRIMER_PAIR_1_COMPL_ANY=3.00
10245
PRIMER_PAIR_1_COMPL_END=1.00
10246
PRIMER_PRODUCT_SIZE_1=170
10247
PRIMER_PAIR_PENALTY_2=3.6589
10248
PRIMER_LEFT_2_PENALTY=3.100969
10249
PRIMER_RIGHT_2_PENALTY=0.557889
10250
PRIMER_LEFT_2_SEQUENCE=TGATGAAGAGTGAAGACACCTGA
10251
PRIMER_RIGHT_2_SEQUENCE=ATGCACATGTGTGTGCATGT
10252
PRIMER_LEFT_2=236,23
10253
PRIMER_RIGHT_2=363,20
10254
PRIMER_LEFT_2_TM=59.899
10255
PRIMER_RIGHT_2_TM=59.442
10256
PRIMER_LEFT_2_GC_PERCENT=43.478
10257
PRIMER_RIGHT_2_GC_PERCENT=45.000
10258
PRIMER_LEFT_2_SELF_ANY=3.00
10259
PRIMER_RIGHT_2_SELF_ANY=10.00
10260
PRIMER_LEFT_2_SELF_END=1.00
10261
PRIMER_RIGHT_2_SELF_END=4.00
10262
PRIMER_LEFT_2_END_STABILITY=8.2000
10263
PRIMER_RIGHT_2_END_STABILITY=6.6000
10264
PRIMER_PAIR_2_COMPL_ANY=6.00
10265
PRIMER_PAIR_2_COMPL_END=4.00
10266
PRIMER_PRODUCT_SIZE_2=128
10267
PRIMER_PAIR_PENALTY_3=3.8234
10268
PRIMER_LEFT_3_PENALTY=3.265553
10269
PRIMER_RIGHT_3_PENALTY=0.557889
10270
PRIMER_LEFT_3_SEQUENCE=GCTAGGTTCAATGAAAAACCC
10271
PRIMER_RIGHT_3_SEQUENCE=ATGCACATGTGTGTGCATGT
10272
PRIMER_LEFT_3=194,21
10273
PRIMER_RIGHT_3=363,20
10274
PRIMER_LEFT_3_TM=57.734
10275
PRIMER_RIGHT_3_TM=59.442
10276
PRIMER_LEFT_3_GC_PERCENT=42.857
10277
PRIMER_RIGHT_3_GC_PERCENT=45.000
10278
PRIMER_LEFT_3_SELF_ANY=7.00
10279
PRIMER_RIGHT_3_SELF_ANY=10.00
10280
PRIMER_LEFT_3_SELF_END=0.00
10281
PRIMER_RIGHT_3_SELF_END=4.00
10282
PRIMER_LEFT_3_END_STABILITY=9.4000
10283
PRIMER_RIGHT_3_END_STABILITY=6.6000
10284
PRIMER_PAIR_3_COMPL_ANY=3.00
10285
PRIMER_PAIR_3_COMPL_END=1.00
10286
PRIMER_PRODUCT_SIZE_3=170
10287
PRIMER_PAIR_PENALTY_4=3.8638
10288
PRIMER_LEFT_4_PENALTY=3.305865
10289
PRIMER_RIGHT_4_PENALTY=0.557889
10290
PRIMER_LEFT_4_SEQUENCE=GCTAGGTTCAATGAAAAACCCT
10291
PRIMER_RIGHT_4_SEQUENCE=ATGCACATGTGTGTGCATGT
10292
PRIMER_LEFT_4=194,22
10293
PRIMER_RIGHT_4=363,20
10294
PRIMER_LEFT_4_TM=58.694
10295
PRIMER_RIGHT_4_TM=59.442
10296
PRIMER_LEFT_4_GC_PERCENT=40.909
10297
PRIMER_RIGHT_4_GC_PERCENT=45.000
10298
PRIMER_LEFT_4_SELF_ANY=7.00
10299
PRIMER_RIGHT_4_SELF_ANY=10.00
10300
PRIMER_LEFT_4_SELF_END=1.00
10301
PRIMER_RIGHT_4_SELF_END=4.00
10302
PRIMER_LEFT_4_END_STABILITY=9.1000
10303
PRIMER_RIGHT_4_END_STABILITY=6.6000
10304
PRIMER_PAIR_4_COMPL_ANY=3.00
10305
PRIMER_PAIR_4_COMPL_END=1.00
10306
PRIMER_PRODUCT_SIZE_4=170
10307
=
10308
MARKER_NAME=13.RHAP69FE1.seq
10309
SEQUENCE=AAACAAACATAAAGTATGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCAGAAAAATGTTCCTGCAGTGATGTGGATGAAGGATAGGGATGGATTGAAGACGCCAGCAGGCTGGCTTTGTGAGAGTCCAAATAAAACCCTGACAGCAGAGGCATGAGAAGGATTCCAGAAATTTTTAGAACATCACAGCCCAGGCTTTGGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
10310
INCLUDED_REGION=0,233
10311
TARGET=20,56,CA
10312
PRIMER_COMMENT=CL is
10313
PRIMER_COMMENT=CR is
10314
=
10315
MARKER_NAME=15.RHAP69FE3.seq
10316
SEQUENCE=NNNNNNNNNNNNNNNNNACACAATTCATTTAAACCACATGCTAGAGTCTACCCTTACAAAAAAAGCAAAAAAGCAAAATATTATAACTTAATTTTTTGATTTCTCTCTTCTATATCATGTCCTACTCTCTCCTTTGTAGATACCATCTCTACCACCTGGTAACACAGCATTTCTTTTTTTCTCTTTCTACATGAATATATCTAGACGACTCAGACACTCATCTACATAAAGAGAAAAATAAGCCAAGACCCTCCCTCCCTCATATGTGTGTGNNNNNNNNNNNNNNNNNNNNNNTTCCATGAGTGTGAAAATGTCCCTGTGGGGAAAATAGCCAGTAGTAGCCAGTATGCAAATTTAAAAAGAAAAGGTTCAGTTCTTCATTCTTCAATTCCTGTCTCACATCATCTTTGTTGCCTTAAGGACAATGAAAGAATCAAAATATCTGCTAGAAACTTTCCTTGGT
10317
INCLUDED_REGION=17,446
10318
TARGET=272,22,TA
10319
PRIMER_COMMENT=CL is CCACCTGGTAACACAGCATTT
10320
PRIMER_COMMENT=CR is CCACAGGGACATTTTCACACT
10321
PRIMER_PAIR_PENALTY=2.2184
10322
PRIMER_LEFT_PENALTY=1.094209
10323
PRIMER_RIGHT_PENALTY=1.124240
10324
PRIMER_LEFT_SEQUENCE=CCACCTGGTAACACAGCATTT
10325
PRIMER_RIGHT_SEQUENCE=CCACAGGGACATTTTCACACT
10326
PRIMER_LEFT=151,21
10327
PRIMER_RIGHT=321,21
10328
PRIMER_LEFT_TM=59.906
10329
PRIMER_RIGHT_TM=59.876
10330
PRIMER_LEFT_GC_PERCENT=47.619
10331
PRIMER_RIGHT_GC_PERCENT=47.619
10332
PRIMER_LEFT_SELF_ANY=5.00
10333
PRIMER_RIGHT_SELF_ANY=3.00
10334
PRIMER_LEFT_SELF_END=0.00
10335
PRIMER_RIGHT_SELF_END=1.00
10336
PRIMER_LEFT_END_STABILITY=7.2000
10337
PRIMER_RIGHT_END_STABILITY=6.1000
10338
PRIMER_PAIR_COMPL_ANY=4.00
10339
PRIMER_PAIR_COMPL_END=0.00
10340
PRIMER_PRODUCT_SIZE=171
10341
PRIMER_PAIR_PENALTY_1=2.2514
10342
PRIMER_LEFT_1_PENALTY=1.094209
10343
PRIMER_RIGHT_1_PENALTY=1.157240
10344
PRIMER_LEFT_1_SEQUENCE=CCACCTGGTAACACAGCATTT
10345
PRIMER_RIGHT_1_SEQUENCE=TTCCCCACAGGGACATTTT
10346
PRIMER_LEFT_1=151,21
10347
PRIMER_RIGHT_1=325,19
10348
PRIMER_LEFT_1_TM=59.906
10349
PRIMER_RIGHT_1_TM=60.157
10350
PRIMER_LEFT_1_GC_PERCENT=47.619
10351
PRIMER_RIGHT_1_GC_PERCENT=47.368
10352
PRIMER_LEFT_1_SELF_ANY=5.00
10353
PRIMER_RIGHT_1_SELF_ANY=4.00
10354
PRIMER_LEFT_1_SELF_END=0.00
10355
PRIMER_RIGHT_1_SELF_END=1.00
10356
PRIMER_LEFT_1_END_STABILITY=7.2000
10357
PRIMER_RIGHT_1_END_STABILITY=7.2000
10358
PRIMER_PAIR_1_COMPL_ANY=4.00
10359
PRIMER_PAIR_1_COMPL_END=1.00
10360
PRIMER_PRODUCT_SIZE_1=175
10361
PRIMER_PAIR_PENALTY_2=2.2672
10362
PRIMER_LEFT_2_PENALTY=1.094209
10363
PRIMER_RIGHT_2_PENALTY=1.172949
10364
PRIMER_LEFT_2_SEQUENCE=CCACCTGGTAACACAGCATTT
10365
PRIMER_RIGHT_2_SEQUENCE=CCACAGGGACATTTTCACAC
10366
PRIMER_LEFT_2=151,21
10367
PRIMER_RIGHT_2=321,20
10368
PRIMER_LEFT_2_TM=59.906
10369
PRIMER_RIGHT_2_TM=58.827
10370
PRIMER_LEFT_2_GC_PERCENT=47.619
10371
PRIMER_RIGHT_2_GC_PERCENT=50.000
10372
PRIMER_LEFT_2_SELF_ANY=5.00
10373
PRIMER_RIGHT_2_SELF_ANY=3.00
10374
PRIMER_LEFT_2_SELF_END=0.00
10375
PRIMER_RIGHT_2_SELF_END=0.00
10376
PRIMER_LEFT_2_END_STABILITY=7.2000
10377
PRIMER_RIGHT_2_END_STABILITY=6.4000
10378
PRIMER_PAIR_2_COMPL_ANY=4.00
10379
PRIMER_PAIR_2_COMPL_END=0.00
10380
PRIMER_PRODUCT_SIZE_2=171
10381
PRIMER_PAIR_PENALTY_3=2.3194
10382
PRIMER_LEFT_3_PENALTY=1.094209
10383
PRIMER_RIGHT_3_PENALTY=1.225160
10384
PRIMER_LEFT_3_SEQUENCE=CCACCTGGTAACACAGCATTT
10385
PRIMER_RIGHT_3_SEQUENCE=GGGACATTTTCACACTCATGG
10386
PRIMER_LEFT_3=151,21
10387
PRIMER_RIGHT_3=316,21
10388
PRIMER_LEFT_3_TM=59.906
10389
PRIMER_RIGHT_3_TM=60.225
10390
PRIMER_LEFT_3_GC_PERCENT=47.619
10391
PRIMER_RIGHT_3_GC_PERCENT=47.619
10392
PRIMER_LEFT_3_SELF_ANY=5.00
10393
PRIMER_RIGHT_3_SELF_ANY=4.00
10394
PRIMER_LEFT_3_SELF_END=0.00
10395
PRIMER_RIGHT_3_SELF_END=2.00
10396
PRIMER_LEFT_3_END_STABILITY=7.2000
10397
PRIMER_RIGHT_3_END_STABILITY=8.4000
10398
PRIMER_PAIR_3_COMPL_ANY=3.00
10399
PRIMER_PAIR_3_COMPL_END=0.00
10400
PRIMER_PRODUCT_SIZE_3=166
10401
PRIMER_PAIR_PENALTY_4=2.3698
10402
PRIMER_LEFT_4_PENALTY=0.110099
10403
PRIMER_RIGHT_4_PENALTY=2.259704
10404
PRIMER_LEFT_4_SEQUENCE=AAGACCCTCCCTCCCTCATA
10405
PRIMER_RIGHT_4_SEQUENCE=AGGCAACAAAGATGATGTGAGA
10406
PRIMER_LEFT_4=244,20
10407
PRIMER_RIGHT_4=415,22
10408
PRIMER_LEFT_4_TM=59.890
10409
PRIMER_RIGHT_4_TM=59.740
10410
PRIMER_LEFT_4_GC_PERCENT=55.000
10411
PRIMER_RIGHT_4_GC_PERCENT=40.909
10412
PRIMER_LEFT_4_SELF_ANY=2.00
10413
PRIMER_RIGHT_4_SELF_ANY=3.00
10414
PRIMER_LEFT_4_SELF_END=2.00
10415
PRIMER_RIGHT_4_SELF_END=1.00
10416
PRIMER_LEFT_4_END_STABILITY=5.9000
10417
PRIMER_RIGHT_4_END_STABILITY=6.7000
10418
PRIMER_PAIR_4_COMPL_ANY=5.00
10419
PRIMER_PAIR_4_COMPL_END=3.00
10420
PRIMER_PRODUCT_SIZE_4=172
10421
=
10422
MARKER_NAME=21.RHAP69FG3.seq
10423
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCCAGCCATTCACAGTGCCTTCTCTGTCTCCTGCAGTCAGTTCAGGATGTAAGCCCTCAGCTACTGCAATGATCCCCAAATGAGAAATGAAATGTCTTAGTGAACTTTTTTTTTCAAAAAGTTAGGTATGCTTAGTGGTAGGAAGATGGCTCATTTGATAAAATGCTTGCCCCACACTCAAGCGTGATGATCTGTGTTTGGTTCTCTTATACCTATGATTAATAACAAAACCAAGAGTGGGGAGATCCTGCCTATTATCCCAACACTAGGAAGGCACAAATAGGAAGATCCCTGAAGCCTACCAACCTACCGTAGTCATTGAGCTCCAGGTTCAGGGAGAGACTCAAAAAATAAGATAGAGAATTGTAGAGAAAAATGTAAGCCCTCAACCTCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAG
10424
INCLUDED_REGION=31,446
10425
TARGET=427,48,AC
10426
PRIMER_COMMENT=CL is
10427
PRIMER_COMMENT=CR is
10428
=
10429
MARKER_NAME=25.RHAP69FA4.seq
10430
SEQUENCE=NNNNNNCCTATAATGTAGGCACTGTTCTGTTTTACAAGTGACACAATTATTTCCTTGGCAGGCACAGAGCCTGAGTGTCCCCTCCAATCCCCCACACCAGGGCTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCATCTCCTACAAGGTGTGTGTGAACGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
10431
INCLUDED_REGION=6,175
10432
TARGET=106,44,GT
10433
PRIMER_COMMENT=CL is AATTATTTCCTTGGCAGGCA
10434
PRIMER_COMMENT=CR is CCACGTTCACACACACCTTG
10435
PRIMER_PAIR_PENALTY=1.5546
10436
PRIMER_LEFT_PENALTY=0.453004
10437
PRIMER_RIGHT_PENALTY=1.101601
10438
PRIMER_LEFT_SEQUENCE=CAATTATTTCCTTGGCAGGC
10439
PRIMER_RIGHT_SEQUENCE=CCACGTTCACACACACCTTG
10440
PRIMER_LEFT=43,20
10441
PRIMER_RIGHT=180,20
10442
PRIMER_LEFT_TM=59.547
10443
PRIMER_RIGHT_TM=61.102
10444
PRIMER_LEFT_GC_PERCENT=45.000
10445
PRIMER_RIGHT_GC_PERCENT=55.000
10446
PRIMER_LEFT_SELF_ANY=5.00
10447
PRIMER_RIGHT_SELF_ANY=4.00
10448
PRIMER_LEFT_SELF_END=2.00
10449
PRIMER_RIGHT_SELF_END=1.00
10450
PRIMER_LEFT_END_STABILITY=9.7000
10451
PRIMER_RIGHT_END_STABILITY=8.5000
10452
PRIMER_PAIR_COMPL_ANY=3.00
10453
PRIMER_PAIR_COMPL_END=1.00
10454
PRIMER_PRODUCT_SIZE=138
10455
PRIMER_PAIR_PENALTY_1=1.5546
10456
PRIMER_LEFT_1_PENALTY=0.453004
10457
PRIMER_RIGHT_1_PENALTY=1.101601
10458
PRIMER_LEFT_1_SEQUENCE=AATTATTTCCTTGGCAGGCA
10459
PRIMER_RIGHT_1_SEQUENCE=CCACGTTCACACACACCTTG
10460
PRIMER_LEFT_1=44,20
10461
PRIMER_RIGHT_1=180,20
10462
PRIMER_LEFT_1_TM=59.547
10463
PRIMER_RIGHT_1_TM=61.102
10464
PRIMER_LEFT_1_GC_PERCENT=40.000
10465
PRIMER_RIGHT_1_GC_PERCENT=55.000
10466
PRIMER_LEFT_1_SELF_ANY=5.00
10467
PRIMER_RIGHT_1_SELF_ANY=4.00
10468
PRIMER_LEFT_1_SELF_END=3.00
10469
PRIMER_RIGHT_1_SELF_END=1.00
10470
PRIMER_LEFT_1_END_STABILITY=9.7000
10471
PRIMER_RIGHT_1_END_STABILITY=8.5000
10472
PRIMER_PAIR_1_COMPL_ANY=3.00
10473
PRIMER_PAIR_1_COMPL_END=2.00
10474
PRIMER_PRODUCT_SIZE_1=137
10475
PRIMER_PAIR_PENALTY_2=2.0552
10476
PRIMER_LEFT_2_PENALTY=0.953558
10477
PRIMER_RIGHT_2_PENALTY=1.101601
10478
PRIMER_LEFT_2_SEQUENCE=ATTATTTCCTTGGCAGGCAC
10479
PRIMER_RIGHT_2_SEQUENCE=CCACGTTCACACACACCTTG
10480
PRIMER_LEFT_2=45,20
10481
PRIMER_RIGHT_2=180,20
10482
PRIMER_LEFT_2_TM=59.046
10483
PRIMER_RIGHT_2_TM=61.102
10484
PRIMER_LEFT_2_GC_PERCENT=45.000
10485
PRIMER_RIGHT_2_GC_PERCENT=55.000
10486
PRIMER_LEFT_2_SELF_ANY=3.00
10487
PRIMER_RIGHT_2_SELF_ANY=4.00
10488
PRIMER_LEFT_2_SELF_END=1.00
10489
PRIMER_RIGHT_2_SELF_END=1.00
10490
PRIMER_LEFT_2_END_STABILITY=9.4000
10491
PRIMER_RIGHT_2_END_STABILITY=8.5000
10492
PRIMER_PAIR_2_COMPL_ANY=3.00
10493
PRIMER_PAIR_2_COMPL_END=1.00
10494
PRIMER_PRODUCT_SIZE_2=136
10495
PRIMER_PAIR_PENALTY_3=2.2585
10496
PRIMER_LEFT_3_PENALTY=1.156894
10497
PRIMER_RIGHT_3_PENALTY=1.101601
10498
PRIMER_LEFT_3_SEQUENCE=TATTTCCTTGGCAGGCACAG
10499
PRIMER_RIGHT_3_SEQUENCE=CCACGTTCACACACACCTTG
10500
PRIMER_LEFT_3=47,20
10501
PRIMER_RIGHT_3=180,20
10502
PRIMER_LEFT_3_TM=61.157
10503
PRIMER_RIGHT_3_TM=61.102
10504
PRIMER_LEFT_3_GC_PERCENT=50.000
10505
PRIMER_RIGHT_3_GC_PERCENT=55.000
10506
PRIMER_LEFT_3_SELF_ANY=3.00
10507
PRIMER_RIGHT_3_SELF_ANY=4.00
10508
PRIMER_LEFT_3_SELF_END=3.00
10509
PRIMER_RIGHT_3_SELF_END=1.00
10510
PRIMER_LEFT_3_END_STABILITY=6.7000
10511
PRIMER_RIGHT_3_END_STABILITY=8.5000
10512
PRIMER_PAIR_3_COMPL_ANY=3.00
10513
PRIMER_PAIR_3_COMPL_END=2.00
10514
PRIMER_PRODUCT_SIZE_3=134
10515
PRIMER_PAIR_PENALTY_4=2.3059
10516
PRIMER_LEFT_4_PENALTY=1.204289
10517
PRIMER_RIGHT_4_PENALTY=1.101601
10518
PRIMER_LEFT_4_SEQUENCE=TATTTCCTTGGCAGGCACA
10519
PRIMER_RIGHT_4_SEQUENCE=CCACGTTCACACACACCTTG
10520
PRIMER_LEFT_4=47,19
10521
PRIMER_RIGHT_4=180,20
10522
PRIMER_LEFT_4_TM=60.204
10523
PRIMER_RIGHT_4_TM=61.102
10524
PRIMER_LEFT_4_GC_PERCENT=47.368
10525
PRIMER_RIGHT_4_GC_PERCENT=55.000
10526
PRIMER_LEFT_4_SELF_ANY=3.00
10527
PRIMER_RIGHT_4_SELF_ANY=4.00
10528
PRIMER_LEFT_4_SELF_END=3.00
10529
PRIMER_RIGHT_4_SELF_END=1.00
10530
PRIMER_LEFT_4_END_STABILITY=8.2000
10531
PRIMER_RIGHT_4_END_STABILITY=8.5000
10532
PRIMER_PAIR_4_COMPL_ANY=3.00
10533
PRIMER_PAIR_4_COMPL_END=2.00
10534
PRIMER_PRODUCT_SIZE_4=134
10535
=
10536
MARKER_NAME=26.RHAP69FA11.seq
10537
SEQUENCE=NNNNNNNNNNNNNNNNNNACTTATTTACAGAGACTTTTAAGGGTGACAGAAGCCTCTGAGTTAAGCCAGTGGGCACTATGTGATTTTCACATCTCCCAGCAGCTCCAGCATTGCTGTGAACTAGGGAGTTGACCAGTCTTCAAGTGATGCTGCTCTCAGAAACATTAGTCAAGAGGGTAGTTTTAAATTGCTGTTTGATTGGTTNNNNNNNNNNNNNNNNNNNTNCCGNCNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTTAAATTAGCTAATCTAGAAACTCACACCTTGTTAAAGTGCAGAGACTAAGTGACCAAGAAGAATGAGTCACAAAGGGGACATCC
10538
INCLUDED_REGION=18,353
10539
TARGET=204,18,TC
10540
TARGET=231,18,CT
10541
TARGET=250,34,TG
10542
PRIMER_COMMENT=CL is TTTTAAATTGCTGTTTGATTGGTT
10543
PRIMER_COMMENT=CR is TCTGCACTTTAACAAGGTGTGA
10544
PRIMER_PAIR_PENALTY=8.4770
10545
PRIMER_LEFT_PENALTY=4.960837
10546
PRIMER_RIGHT_PENALTY=3.516163
10547
PRIMER_LEFT_SEQUENCE=TTTTAAATTGCTGTTTGATTGGTT
10548
PRIMER_RIGHT_SEQUENCE=TCTGCACTTTAACAAGGTGTGA
10549
PRIMER_LEFT=180,24
10550
PRIMER_RIGHT=329,22
10551
PRIMER_LEFT_TM=59.039
10552
PRIMER_RIGHT_TM=58.484
10553
PRIMER_LEFT_GC_PERCENT=25.000
10554
PRIMER_RIGHT_GC_PERCENT=40.909
10555
PRIMER_LEFT_SELF_ANY=6.00
10556
PRIMER_RIGHT_SELF_ANY=5.00
10557
PRIMER_LEFT_SELF_END=0.00
10558
PRIMER_RIGHT_SELF_END=3.00
10559
PRIMER_LEFT_END_STABILITY=8.2000
10560
PRIMER_RIGHT_END_STABILITY=6.7000
10561
PRIMER_PAIR_COMPL_ANY=7.00
10562
PRIMER_PAIR_COMPL_END=1.00
10563
PRIMER_PRODUCT_SIZE=150
10564
PRIMER_PAIR_PENALTY_1=8.4863
10565
PRIMER_LEFT_1_PENALTY=4.960837
10566
PRIMER_RIGHT_1_PENALTY=3.525488
10567
PRIMER_LEFT_1_SEQUENCE=TTTTAAATTGCTGTTTGATTGGTT
10568
PRIMER_RIGHT_1_SEQUENCE=TCTCTGCACTTTAACAAGGTGTG
10569
PRIMER_LEFT_1=180,24
10570
PRIMER_RIGHT_1=331,23
10571
PRIMER_LEFT_1_TM=59.039
10572
PRIMER_RIGHT_1_TM=59.475
10573
PRIMER_LEFT_1_GC_PERCENT=25.000
10574
PRIMER_RIGHT_1_GC_PERCENT=43.478
10575
PRIMER_LEFT_1_SELF_ANY=6.00
10576
PRIMER_RIGHT_1_SELF_ANY=5.00
10577
PRIMER_LEFT_1_SELF_END=0.00
10578
PRIMER_RIGHT_1_SELF_END=1.00
10579
PRIMER_LEFT_1_END_STABILITY=8.2000
10580
PRIMER_RIGHT_1_END_STABILITY=6.4000
10581
PRIMER_PAIR_1_COMPL_ANY=7.00
10582
PRIMER_PAIR_1_COMPL_END=0.00
10583
PRIMER_PRODUCT_SIZE_1=152
10584
PRIMER_PAIR_PENALTY_2=8.4863
10585
PRIMER_LEFT_2_PENALTY=4.960837
10586
PRIMER_RIGHT_2_PENALTY=3.525488
10587
PRIMER_LEFT_2_SEQUENCE=TTTTAAATTGCTGTTTGATTGGTT
10588
PRIMER_RIGHT_2_SEQUENCE=TCTGCACTTTAACAAGGTGTGAG
10589
PRIMER_LEFT_2=180,24
10590
PRIMER_RIGHT_2=329,23
10591
PRIMER_LEFT_2_TM=59.039
10592
PRIMER_RIGHT_2_TM=59.475
10593
PRIMER_LEFT_2_GC_PERCENT=25.000
10594
PRIMER_RIGHT_2_GC_PERCENT=43.478
10595
PRIMER_LEFT_2_SELF_ANY=6.00
10596
PRIMER_RIGHT_2_SELF_ANY=5.00
10597
PRIMER_LEFT_2_SELF_END=0.00
10598
PRIMER_RIGHT_2_SELF_END=3.00
10599
PRIMER_LEFT_2_END_STABILITY=8.2000
10600
PRIMER_RIGHT_2_END_STABILITY=6.4000
10601
PRIMER_PAIR_2_COMPL_ANY=7.00
10602
PRIMER_PAIR_2_COMPL_END=0.00
10603
PRIMER_PRODUCT_SIZE_2=150
10604
PRIMER_PAIR_PENALTY_3=8.4863
10605
PRIMER_LEFT_3_PENALTY=4.960837
10606
PRIMER_RIGHT_3_PENALTY=3.525488
10607
PRIMER_LEFT_3_SEQUENCE=TTTTAAATTGCTGTTTGATTGGTT
10608
PRIMER_RIGHT_3_SEQUENCE=CTCTGCACTTTAACAAGGTGTGA
10609
PRIMER_LEFT_3=180,24
10610
PRIMER_RIGHT_3=330,23
10611
PRIMER_LEFT_3_TM=59.039
10612
PRIMER_RIGHT_3_TM=59.475
10613
PRIMER_LEFT_3_GC_PERCENT=25.000
10614
PRIMER_RIGHT_3_GC_PERCENT=43.478
10615
PRIMER_LEFT_3_SELF_ANY=6.00
10616
PRIMER_RIGHT_3_SELF_ANY=5.00
10617
PRIMER_LEFT_3_SELF_END=0.00
10618
PRIMER_RIGHT_3_SELF_END=3.00
10619
PRIMER_LEFT_3_END_STABILITY=8.2000
10620
PRIMER_RIGHT_3_END_STABILITY=6.7000
10621
PRIMER_PAIR_3_COMPL_ANY=7.00
10622
PRIMER_PAIR_3_COMPL_END=1.00
10623
PRIMER_PRODUCT_SIZE_3=151
10624
PRIMER_PAIR_PENALTY_4=8.5811
10625
PRIMER_LEFT_4_PENALTY=5.064919
10626
PRIMER_RIGHT_4_PENALTY=3.516163
10627
PRIMER_LEFT_4_SEQUENCE=TCAAGAGGGTAGTTTTAAATTGCTG
10628
PRIMER_RIGHT_4_SEQUENCE=TCTGCACTTTAACAAGGTGTGA
10629
PRIMER_LEFT_4=168,25
10630
PRIMER_RIGHT_4=329,22
10631
PRIMER_LEFT_4_TM=60.065
10632
PRIMER_RIGHT_4_TM=58.484
10633
PRIMER_LEFT_4_GC_PERCENT=36.000
10634
PRIMER_RIGHT_4_GC_PERCENT=40.909
10635
PRIMER_LEFT_4_SELF_ANY=6.00
10636
PRIMER_RIGHT_4_SELF_ANY=5.00
10637
PRIMER_LEFT_4_SELF_END=1.00
10638
PRIMER_RIGHT_4_SELF_END=3.00
10639
PRIMER_LEFT_4_END_STABILITY=8.5000
10640
PRIMER_RIGHT_4_END_STABILITY=6.7000
10641
PRIMER_PAIR_4_COMPL_ANY=7.00
10642
PRIMER_PAIR_4_COMPL_END=1.00
10643
PRIMER_PRODUCT_SIZE_4=162
10644
=
10645
MARKER_NAME=27.RHAP69FA6.seq
10646
SEQUENCE=NNNNNNNNNNNNNNNNNNNACCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCTATACTTCCTTTCAATTTTTAGCACTTCNAGTCATACATAGGACATAGTCACACATTTCCATTATGCTATAAAATATTGTCAAAATTTAAATAAGTCATCATGGTTGAAGTTATTTCATTTGCTATCTCTAAGATTAGAGCATGCTCTAATATCTTCCTGGTTCCAGAAAATTCAGACCTGTTTTTATCTTATTAAATACCCTCAAGCTCAGCTCATTCTTGAACTGTTGAACTGCAGCCAAGGTGAAGACATTCACATACAAATTAAACATTTTATTTTGCTAATGTTTTTGTGACTGCTTACTACTCAGCAGTAGCTCCTCAAACCAAAAACATTTGATGAGCCATGACTTTGTCATAAATAAGAAAAC
10647
INCLUDED_REGION=19,447
10648
TARGET=23,69,AC
10649
PRIMER_COMMENT=CL is
10650
PRIMER_COMMENT=CR is
10651
=
10652
MARKER_NAME=31.RHAP69FC10.seq
10653
SEQUENCE=NNNNNNNNNNNNNNNNNNACTTAATNTGTTTTTATGATATATTGAATCTCGGTCTCATAATGAGACAGAACATGTGACATTTATTTTCTTCCCCAGTTATTCTATCTTGTTCCCAGTTCTCTTCCTTTGAACAATCAGTCTCCCTTTACAATCTTTTTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGCAGAATACACCNAGAAATTTCAGTCCGGTGNGCGGTTGATTTCTGGGTATGTCCATATATGTANGTATGTGTATGTGAGTGTCTAGAGAGTTCACAAGAATGCATAGGCTCTTGGGGCTGAAGCTACAGGAAGTTCTCACTTCTCCAATTTGAGTGCTAAGGCATGGA
10654
INCLUDED_REGION=18,354
10655
TARGET=160,42,TG
10656
PRIMER_COMMENT=CL is TCCCAGTTCTCTTCCTTTGAA
10657
PRIMER_COMMENT=CR is CATACCCAGAAATCAACCGC
10658
PRIMER_PAIR_PENALTY=2.4359
10659
PRIMER_LEFT_PENALTY=2.105078
10660
PRIMER_RIGHT_PENALTY=0.330865
10661
PRIMER_LEFT_SEQUENCE=TTCCCAGTTCTCTTCCTTTGA
10662
PRIMER_RIGHT_SEQUENCE=CATACCCAGAAATCAACCGC
10663
PRIMER_LEFT=109,21
10664
PRIMER_RIGHT=254,20
10665
PRIMER_LEFT_TM=58.895
10666
PRIMER_RIGHT_TM=60.331
10667
PRIMER_LEFT_GC_PERCENT=42.857
10668
PRIMER_RIGHT_GC_PERCENT=50.000
10669
PRIMER_LEFT_SELF_ANY=2.00
10670
PRIMER_RIGHT_SELF_ANY=2.00
10671
PRIMER_LEFT_SELF_END=1.00
10672
PRIMER_RIGHT_SELF_END=2.00
10673
PRIMER_LEFT_END_STABILITY=7.3000
10674
PRIMER_RIGHT_END_STABILITY=11.1000
10675
PRIMER_PAIR_COMPL_ANY=4.00
10676
PRIMER_PAIR_COMPL_END=2.00
10677
PRIMER_PRODUCT_SIZE=146
10678
PRIMER_PAIR_PENALTY_1=2.4359
10679
PRIMER_LEFT_1_PENALTY=2.105078
10680
PRIMER_RIGHT_1_PENALTY=0.330865
10681
PRIMER_LEFT_1_SEQUENCE=TCCCAGTTCTCTTCCTTTGAA
10682
PRIMER_RIGHT_1_SEQUENCE=CATACCCAGAAATCAACCGC
10683
PRIMER_LEFT_1=110,21
10684
PRIMER_RIGHT_1=254,20
10685
PRIMER_LEFT_1_TM=58.895
10686
PRIMER_RIGHT_1_TM=60.331
10687
PRIMER_LEFT_1_GC_PERCENT=42.857
10688
PRIMER_RIGHT_1_GC_PERCENT=50.000
10689
PRIMER_LEFT_1_SELF_ANY=3.00
10690
PRIMER_RIGHT_1_SELF_ANY=2.00
10691
PRIMER_LEFT_1_SELF_END=3.00
10692
PRIMER_RIGHT_1_SELF_END=2.00
10693
PRIMER_LEFT_1_END_STABILITY=7.3000
10694
PRIMER_RIGHT_1_END_STABILITY=11.1000
10695
PRIMER_PAIR_1_COMPL_ANY=4.00
10696
PRIMER_PAIR_1_COMPL_END=1.00
10697
PRIMER_PRODUCT_SIZE_1=145
10698
PRIMER_PAIR_PENALTY_2=2.5444
10699
PRIMER_LEFT_2_PENALTY=2.213575
10700
PRIMER_RIGHT_2_PENALTY=0.330865
10701
PRIMER_LEFT_2_SEQUENCE=TTCCCAGTTCTCTTCCTTTGAA
10702
PRIMER_RIGHT_2_SEQUENCE=CATACCCAGAAATCAACCGC
10703
PRIMER_LEFT_2=109,22
10704
PRIMER_RIGHT_2=254,20
10705
PRIMER_LEFT_2_TM=60.214
10706
PRIMER_RIGHT_2_TM=60.331
10707
PRIMER_LEFT_2_GC_PERCENT=40.909
10708
PRIMER_RIGHT_2_GC_PERCENT=50.000
10709
PRIMER_LEFT_2_SELF_ANY=3.00
10710
PRIMER_RIGHT_2_SELF_ANY=2.00
10711
PRIMER_LEFT_2_SELF_END=3.00
10712
PRIMER_RIGHT_2_SELF_END=2.00
10713
PRIMER_LEFT_2_END_STABILITY=7.3000
10714
PRIMER_RIGHT_2_END_STABILITY=11.1000
10715
PRIMER_PAIR_2_COMPL_ANY=4.00
10716
PRIMER_PAIR_2_COMPL_END=1.00
10717
PRIMER_PRODUCT_SIZE_2=146
10718
PRIMER_PAIR_PENALTY_3=2.6016
10719
PRIMER_LEFT_3_PENALTY=2.270774
10720
PRIMER_RIGHT_3_PENALTY=0.330865
10721
PRIMER_LEFT_3_SEQUENCE=TCCCAGTTCTCTTCCTTTGAAC
10722
PRIMER_RIGHT_3_SEQUENCE=CATACCCAGAAATCAACCGC
10723
PRIMER_LEFT_3=110,22
10724
PRIMER_RIGHT_3=254,20
10725
PRIMER_LEFT_3_TM=59.729
10726
PRIMER_RIGHT_3_TM=60.331
10727
PRIMER_LEFT_3_GC_PERCENT=45.455
10728
PRIMER_RIGHT_3_GC_PERCENT=50.000
10729
PRIMER_LEFT_3_SELF_ANY=4.00
10730
PRIMER_RIGHT_3_SELF_ANY=2.00
10731
PRIMER_LEFT_3_SELF_END=1.00
10732
PRIMER_RIGHT_3_SELF_END=2.00
10733
PRIMER_LEFT_3_END_STABILITY=6.7000
10734
PRIMER_RIGHT_3_END_STABILITY=11.1000
10735
PRIMER_PAIR_3_COMPL_ANY=4.00
10736
PRIMER_PAIR_3_COMPL_END=0.00
10737
PRIMER_PRODUCT_SIZE_3=145
10738
PRIMER_PAIR_PENALTY_4=2.6016
10739
PRIMER_LEFT_4_PENALTY=2.270774
10740
PRIMER_RIGHT_4_PENALTY=0.330865
10741
PRIMER_LEFT_4_SEQUENCE=GTTCCCAGTTCTCTTCCTTTGA
10742
PRIMER_RIGHT_4_SEQUENCE=CATACCCAGAAATCAACCGC
10743
PRIMER_LEFT_4=108,22
10744
PRIMER_RIGHT_4=254,20
10745
PRIMER_LEFT_4_TM=59.729
10746
PRIMER_RIGHT_4_TM=60.331
10747
PRIMER_LEFT_4_GC_PERCENT=45.455
10748
PRIMER_RIGHT_4_GC_PERCENT=50.000
10749
PRIMER_LEFT_4_SELF_ANY=2.00
10750
PRIMER_RIGHT_4_SELF_ANY=2.00
10751
PRIMER_LEFT_4_SELF_END=1.00
10752
PRIMER_RIGHT_4_SELF_END=2.00
10753
PRIMER_LEFT_4_END_STABILITY=7.3000
10754
PRIMER_RIGHT_4_END_STABILITY=11.1000
10755
PRIMER_PAIR_4_COMPL_ANY=4.00
10756
PRIMER_PAIR_4_COMPL_END=2.00
10757
PRIMER_PRODUCT_SIZE_4=147
10758
=
10759
MARKER_NAME=32.RHAP69FC11.seq
10760
SEQUENCE=ANANTAAAGTCCCANCNNNTGTGGGNNGGAGGGTATCCTGGTTACATCAAACCTCTGCTGACTGAGGGCAATAGGGGCAGTATCAGAGAGGCCAAGAAAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGGGGGGTGGAGGGTGCAGGATGATCCATGAGGGGAATCAGTTGTTGCTTCTGGAAAGATTGGGGTAACAGTCCAGAATATTCATATAGGCAGCAGCCCTGGTGGTTAGTGATGCTGGGATTTAAATCAGGGCTTCAACAGTGTTCGAAAATGTGAGGAAGGCGTCTGTTTCTCACCTCTGATCCTTATTATCAAGTCAGGAGAACTTT
10761
INCLUDED_REGION=0,362
10762
TARGET=101,52,TG
10763
PRIMER_COMMENT=CL is AGGGCAATAGGGGCAGTATC
10764
PRIMER_COMMENT=CR is TGATTCCCCTCATGGATCAT
10765
PRIMER_PAIR_PENALTY=0.4029
10766
PRIMER_LEFT_PENALTY=0.306903
10767
PRIMER_RIGHT_PENALTY=0.096021
10768
PRIMER_LEFT_SEQUENCE=AGGGCAATAGGGGCAGTATC
10769
PRIMER_RIGHT_SEQUENCE=TGATTCCCCTCATGGATCAT
10770
PRIMER_LEFT=64,20
10771
PRIMER_RIGHT=192,20
10772
PRIMER_LEFT_TM=60.307
10773
PRIMER_RIGHT_TM=60.096
10774
PRIMER_LEFT_GC_PERCENT=55.000
10775
PRIMER_RIGHT_GC_PERCENT=45.000
10776
PRIMER_LEFT_SELF_ANY=3.00
10777
PRIMER_RIGHT_SELF_ANY=4.00
10778
PRIMER_LEFT_SELF_END=1.00
10779
PRIMER_RIGHT_SELF_END=4.00
10780
PRIMER_LEFT_END_STABILITY=5.3000
10781
PRIMER_RIGHT_END_STABILITY=6.5000
10782
PRIMER_PAIR_COMPL_ANY=6.00
10783
PRIMER_PAIR_COMPL_END=2.00
10784
PRIMER_PRODUCT_SIZE=129
10785
PRIMER_PAIR_PENALTY_1=0.4029
10786
PRIMER_LEFT_1_PENALTY=0.306903
10787
PRIMER_RIGHT_1_PENALTY=0.096021
10788
PRIMER_LEFT_1_SEQUENCE=GAGGGCAATAGGGGCAGTAT
10789
PRIMER_RIGHT_1_SEQUENCE=TGATTCCCCTCATGGATCAT
10790
PRIMER_LEFT_1=63,20
10791
PRIMER_RIGHT_1=192,20
10792
PRIMER_LEFT_1_TM=60.307
10793
PRIMER_RIGHT_1_TM=60.096
10794
PRIMER_LEFT_1_GC_PERCENT=55.000
10795
PRIMER_RIGHT_1_GC_PERCENT=45.000
10796
PRIMER_LEFT_1_SELF_ANY=3.00
10797
PRIMER_RIGHT_1_SELF_ANY=4.00
10798
PRIMER_LEFT_1_SELF_END=3.00
10799
PRIMER_RIGHT_1_SELF_END=4.00
10800
PRIMER_LEFT_1_END_STABILITY=5.3000
10801
PRIMER_RIGHT_1_END_STABILITY=6.5000
10802
PRIMER_PAIR_1_COMPL_ANY=7.00
10803
PRIMER_PAIR_1_COMPL_END=2.00
10804
PRIMER_PRODUCT_SIZE_1=130
10805
PRIMER_PAIR_PENALTY_2=0.4908
10806
PRIMER_LEFT_2_PENALTY=0.306903
10807
PRIMER_RIGHT_2_PENALTY=0.183944
10808
PRIMER_LEFT_2_SEQUENCE=AGGGCAATAGGGGCAGTATC
10809
PRIMER_RIGHT_2_SEQUENCE=CCCAATCTTTCCAGAAGCAA
10810
PRIMER_LEFT_2=64,20
10811
PRIMER_RIGHT_2=216,20
10812
PRIMER_LEFT_2_TM=60.307
10813
PRIMER_RIGHT_2_TM=60.184
10814
PRIMER_LEFT_2_GC_PERCENT=55.000
10815
PRIMER_RIGHT_2_GC_PERCENT=45.000
10816
PRIMER_LEFT_2_SELF_ANY=3.00
10817
PRIMER_RIGHT_2_SELF_ANY=4.00
10818
PRIMER_LEFT_2_SELF_END=1.00
10819
PRIMER_RIGHT_2_SELF_END=0.00
10820
PRIMER_LEFT_2_END_STABILITY=5.3000
10821
PRIMER_RIGHT_2_END_STABILITY=8.5000
10822
PRIMER_PAIR_2_COMPL_ANY=4.00
10823
PRIMER_PAIR_2_COMPL_END=0.00
10824
PRIMER_PRODUCT_SIZE_2=153
10825
PRIMER_PAIR_PENALTY_3=0.4908
10826
PRIMER_LEFT_3_PENALTY=0.306903
10827
PRIMER_RIGHT_3_PENALTY=0.183944
10828
PRIMER_LEFT_3_SEQUENCE=GAGGGCAATAGGGGCAGTAT
10829
PRIMER_RIGHT_3_SEQUENCE=CCCAATCTTTCCAGAAGCAA
10830
PRIMER_LEFT_3=63,20
10831
PRIMER_RIGHT_3=216,20
10832
PRIMER_LEFT_3_TM=60.307
10833
PRIMER_RIGHT_3_TM=60.184
10834
PRIMER_LEFT_3_GC_PERCENT=55.000
10835
PRIMER_RIGHT_3_GC_PERCENT=45.000
10836
PRIMER_LEFT_3_SELF_ANY=3.00
10837
PRIMER_RIGHT_3_SELF_ANY=4.00
10838
PRIMER_LEFT_3_SELF_END=3.00
10839
PRIMER_RIGHT_3_SELF_END=0.00
10840
PRIMER_LEFT_3_END_STABILITY=5.3000
10841
PRIMER_RIGHT_3_END_STABILITY=8.5000
10842
PRIMER_PAIR_3_COMPL_ANY=4.00
10843
PRIMER_PAIR_3_COMPL_END=1.00
10844
PRIMER_PRODUCT_SIZE_3=154
10845
PRIMER_PAIR_PENALTY_4=0.5277
10846
PRIMER_LEFT_4_PENALTY=0.306903
10847
PRIMER_RIGHT_4_PENALTY=0.220845
10848
PRIMER_LEFT_4_SEQUENCE=AGGGCAATAGGGGCAGTATC
10849
PRIMER_RIGHT_4_SEQUENCE=ACAACTGATTCCCCTCATGG
10850
PRIMER_LEFT_4=64,20
10851
PRIMER_RIGHT_4=197,20
10852
PRIMER_LEFT_4_TM=60.307
10853
PRIMER_RIGHT_4_TM=59.779
10854
PRIMER_LEFT_4_GC_PERCENT=55.000
10855
PRIMER_RIGHT_4_GC_PERCENT=50.000
10856
PRIMER_LEFT_4_SELF_ANY=3.00
10857
PRIMER_RIGHT_4_SELF_ANY=4.00
10858
PRIMER_LEFT_4_SELF_END=1.00
10859
PRIMER_RIGHT_4_SELF_END=3.00
10860
PRIMER_LEFT_4_END_STABILITY=5.3000
10861
PRIMER_RIGHT_4_END_STABILITY=8.4000
10862
PRIMER_PAIR_4_COMPL_ANY=6.00
10863
PRIMER_PAIR_4_COMPL_END=2.00
10864
PRIMER_PRODUCT_SIZE_4=134
10865
=
10866
MARKER_NAME=33.RHAP69FC12.seq
10867
SEQUENCE=NNNNNNNNNNNNNNNNNNNGCCTCCCAAGTCCCCTCAGCCTCCTCACCACCTTTTATCAGATGGAGTGGAGGAGATGGCAGTCTCTCCCTCCCAGAAGGCTTTGCAGGCTGCTGAAGCCCTTTCCTTCAGGCTGAGCAGCAGCAGCAGCAGCCCCCAACTGGGGCTGTATTATCATTCCATCTTGACCTCAAAGGAAAGAAAGTGAAGCCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAACTCACTCACATACTCACACGCGCGCGCNNNNNNNNNNNNNNNNCCCTGTCACCCCAGGCCTCTTATCTTGCTGAATCTGCACCATCTCTCATGAAAGAGGCATGCTT
10868
INCLUDED_REGION=19,357
10869
TARGET=212,54,AC
10870
TARGET=296,16,CA
10871
PRIMER_COMMENT=CL is CTTTCCTTCAGGCTGAGCAG
10872
PRIMER_COMMENT=CR is GCGCGTGTGAGTATGTGAGT
10873
PRIMER_PAIR_PENALTY=0.3324
10874
PRIMER_LEFT_PENALTY=0.268570
10875
PRIMER_RIGHT_PENALTY=0.063784
10876
PRIMER_LEFT_SEQUENCE=CTTTCCTTCAGGCTGAGCAG
10877
PRIMER_RIGHT_SEQUENCE=GCGCGTGTGAGTATGTGAGT
10878
PRIMER_LEFT=119,20
10879
PRIMER_RIGHT=291,20
10880
PRIMER_LEFT_TM=60.269
10881
PRIMER_RIGHT_TM=59.936
10882
PRIMER_LEFT_GC_PERCENT=55.000
10883
PRIMER_RIGHT_GC_PERCENT=55.000
10884
PRIMER_LEFT_SELF_ANY=7.00
10885
PRIMER_RIGHT_SELF_ANY=4.00
10886
PRIMER_LEFT_SELF_END=4.00
10887
PRIMER_RIGHT_SELF_END=1.00
10888
PRIMER_LEFT_END_STABILITY=8.2000
10889
PRIMER_RIGHT_END_STABILITY=6.4000
10890
PRIMER_PAIR_COMPL_ANY=3.00
10891
PRIMER_PAIR_COMPL_END=0.00
10892
PRIMER_PRODUCT_SIZE=173
10893
PRIMER_PAIR_PENALTY_1=1.1359
10894
PRIMER_LEFT_1_PENALTY=1.072106
10895
PRIMER_RIGHT_1_PENALTY=0.063784
10896
PRIMER_LEFT_1_SEQUENCE=CCCTTTCCTTCAGGCTGAG
10897
PRIMER_RIGHT_1_SEQUENCE=GCGCGTGTGAGTATGTGAGT
10898
PRIMER_LEFT_1=117,19
10899
PRIMER_RIGHT_1=291,20
10900
PRIMER_LEFT_1_TM=59.928
10901
PRIMER_RIGHT_1_TM=59.936
10902
PRIMER_LEFT_1_GC_PERCENT=57.895
10903
PRIMER_RIGHT_1_GC_PERCENT=55.000
10904
PRIMER_LEFT_1_SELF_ANY=7.00
10905
PRIMER_RIGHT_1_SELF_ANY=4.00
10906
PRIMER_LEFT_1_SELF_END=5.00
10907
PRIMER_RIGHT_1_SELF_END=1.00
10908
PRIMER_LEFT_1_END_STABILITY=6.7000
10909
PRIMER_RIGHT_1_END_STABILITY=6.4000
10910
PRIMER_PAIR_1_COMPL_ANY=3.00
10911
PRIMER_PAIR_1_COMPL_END=0.00
10912
PRIMER_PRODUCT_SIZE_1=175
10913
PRIMER_PAIR_PENALTY_2=1.1444
10914
PRIMER_LEFT_2_PENALTY=1.080649
10915
PRIMER_RIGHT_2_PENALTY=0.063784
10916
PRIMER_LEFT_2_SEQUENCE=CCTTTCCTTCAGGCTGAGC
10917
PRIMER_RIGHT_2_SEQUENCE=GCGCGTGTGAGTATGTGAGT
10918
PRIMER_LEFT_2=118,19
10919
PRIMER_RIGHT_2=291,20
10920
PRIMER_LEFT_2_TM=60.081
10921
PRIMER_RIGHT_2_TM=59.936
10922
PRIMER_LEFT_2_GC_PERCENT=57.895
10923
PRIMER_RIGHT_2_GC_PERCENT=55.000
10924
PRIMER_LEFT_2_SELF_ANY=7.00
10925
PRIMER_RIGHT_2_SELF_ANY=4.00
10926
PRIMER_LEFT_2_SELF_END=5.00
10927
PRIMER_RIGHT_2_SELF_END=1.00
10928
PRIMER_LEFT_2_END_STABILITY=8.2000
10929
PRIMER_RIGHT_2_END_STABILITY=6.4000
10930
PRIMER_PAIR_2_COMPL_ANY=3.00
10931
PRIMER_PAIR_2_COMPL_END=0.00
10932
PRIMER_PRODUCT_SIZE_2=174
10933
PRIMER_PAIR_PENALTY_3=1.2239
10934
PRIMER_LEFT_3_PENALTY=1.160134
10935
PRIMER_RIGHT_3_PENALTY=0.063784
10936
PRIMER_LEFT_3_SEQUENCE=TGGGGCTGTATTATCATTCCA
10937
PRIMER_RIGHT_3_SEQUENCE=GCGCGTGTGAGTATGTGAGT
10938
PRIMER_LEFT_3=159,21
10939
PRIMER_RIGHT_3=291,20
10940
PRIMER_LEFT_3_TM=60.160
10941
PRIMER_RIGHT_3_TM=59.936
10942
PRIMER_LEFT_3_GC_PERCENT=42.857
10943
PRIMER_RIGHT_3_GC_PERCENT=55.000
10944
PRIMER_LEFT_3_SELF_ANY=4.00
10945
PRIMER_RIGHT_3_SELF_ANY=4.00
10946
PRIMER_LEFT_3_SELF_END=2.00
10947
PRIMER_RIGHT_3_SELF_END=1.00
10948
PRIMER_LEFT_3_END_STABILITY=8.5000
10949
PRIMER_RIGHT_3_END_STABILITY=6.4000
10950
PRIMER_PAIR_3_COMPL_ANY=3.00
10951
PRIMER_PAIR_3_COMPL_END=2.00
10952
PRIMER_PRODUCT_SIZE_3=133
10953
PRIMER_PAIR_PENALTY_4=1.2328
10954
PRIMER_LEFT_4_PENALTY=1.169021
10955
PRIMER_RIGHT_4_PENALTY=0.063784
10956
PRIMER_LEFT_4_SEQUENCE=CCAACTGGGGCTGTATTATCA
10957
PRIMER_RIGHT_4_SEQUENCE=GCGCGTGTGAGTATGTGAGT
10958
PRIMER_LEFT_4=154,21
10959
PRIMER_RIGHT_4=291,20
10960
PRIMER_LEFT_4_TM=59.831
10961
PRIMER_RIGHT_4_TM=59.936
10962
PRIMER_LEFT_4_GC_PERCENT=47.619
10963
PRIMER_RIGHT_4_GC_PERCENT=55.000
10964
PRIMER_LEFT_4_SELF_ANY=4.00
10965
PRIMER_RIGHT_4_SELF_ANY=4.00
10966
PRIMER_LEFT_4_SELF_END=3.00
10967
PRIMER_RIGHT_4_SELF_END=1.00
10968
PRIMER_LEFT_4_END_STABILITY=5.9000
10969
PRIMER_RIGHT_4_END_STABILITY=6.4000
10970
PRIMER_PAIR_4_COMPL_ANY=3.00
10971
PRIMER_PAIR_4_COMPL_END=2.00
10972
PRIMER_PRODUCT_SIZE_4=138
10973
=
10974
MARKER_NAME=33.RHAP69FC6.seq
10975
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTTTCTTTGTCCTGTGGCTCAGTTCAAACCATTCATCTCAGAGAAAAAAGCAGAATGGTATATGATAGGCTTGAAAAGTGGGACTTAGAGGATGAATAAGGAGAATAAAGGAATAGAAGACCATACTTAGGCGGAATAACTTCTAGTGTTCAATATGGTGTCTGACACTAATTAAGTGTATATTATATTGCCTATATTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATAGCCACTTACAGAGAACTGAAGACATGAGGCAGAGTGAAAGGTCAAAACATTTGCAGATGAAGCAACCTTGACCCAGTGACTGCAGACTCACTTTTGTTCTATCCCATCCAGTTCCTCAAAACTCAGACGGCCTGACACTCACTAGCTAAAAATAACATGATCTTTTGCAAAGGAAATATTTAAGGCTTCCTATTA
10976
INCLUDED_REGION=31,449
10977
TARGET=231,50,CA
10978
PRIMER_COMMENT=CL is GGCGGAATAACTTCTAGTGTTCA
10979
PRIMER_COMMENT=CR is TGACCTTTCACTCTGCCTCA
10980
PRIMER_PAIR_PENALTY=3.7749
10981
PRIMER_LEFT_PENALTY=3.320295
10982
PRIMER_RIGHT_PENALTY=0.454603
10983
PRIMER_LEFT_SEQUENCE=GGCGGAATAACTTCTAGTGTTCA
10984
PRIMER_RIGHT_SEQUENCE=TGACCTTTCACTCTGCCTCA
10985
PRIMER_LEFT=161,23
10986
PRIMER_RIGHT=328,20
10987
PRIMER_LEFT_TM=59.680
10988
PRIMER_RIGHT_TM=59.545
10989
PRIMER_LEFT_GC_PERCENT=43.478
10990
PRIMER_RIGHT_GC_PERCENT=50.000
10991
PRIMER_LEFT_SELF_ANY=4.00
10992
PRIMER_RIGHT_SELF_ANY=3.00
10993
PRIMER_LEFT_SELF_END=2.00
10994
PRIMER_RIGHT_SELF_END=1.00
10995
PRIMER_LEFT_END_STABILITY=6.7000
10996
PRIMER_RIGHT_END_STABILITY=8.2000
10997
PRIMER_PAIR_COMPL_ANY=4.00
10998
PRIMER_PAIR_COMPL_END=1.00
10999
PRIMER_PRODUCT_SIZE=168
11000
PRIMER_PAIR_PENALTY_1=4.0982
11001
PRIMER_LEFT_1_PENALTY=2.659428
11002
PRIMER_RIGHT_1_PENALTY=1.438803
11003
PRIMER_LEFT_1_SEQUENCE=GAAGACCATACTTAGGCGGAA
11004
PRIMER_RIGHT_1_SEQUENCE=TCACTCTGCCTCATGTCTTCA
11005
PRIMER_LEFT_1=147,21
11006
PRIMER_RIGHT_1=321,21
11007
PRIMER_LEFT_1_TM=58.341
11008
PRIMER_RIGHT_1_TM=59.561
11009
PRIMER_LEFT_1_GC_PERCENT=47.619
11010
PRIMER_RIGHT_1_GC_PERCENT=47.619
11011
PRIMER_LEFT_1_SELF_ANY=3.00
11012
PRIMER_RIGHT_1_SELF_ANY=4.00
11013
PRIMER_LEFT_1_SELF_END=2.00
11014
PRIMER_RIGHT_1_SELF_END=1.00
11015
PRIMER_LEFT_1_END_STABILITY=10.2000
11016
PRIMER_RIGHT_1_END_STABILITY=7.0000
11017
PRIMER_PAIR_1_COMPL_ANY=6.00
11018
PRIMER_PAIR_1_COMPL_END=2.00
11019
PRIMER_PRODUCT_SIZE_1=175
11020
PRIMER_PAIR_PENALTY_2=4.4612
11021
PRIMER_LEFT_2_PENALTY=3.320295
11022
PRIMER_RIGHT_2_PENALTY=1.140954
11023
PRIMER_LEFT_2_SEQUENCE=GGCGGAATAACTTCTAGTGTTCA
11024
PRIMER_RIGHT_2_SEQUENCE=CCTTTCACTCTGCCTCATGTC
11025
PRIMER_LEFT_2=161,23
11026
PRIMER_RIGHT_2=325,21
11027
PRIMER_LEFT_2_TM=59.680
11028
PRIMER_RIGHT_2_TM=59.859
11029
PRIMER_LEFT_2_GC_PERCENT=43.478
11030
PRIMER_RIGHT_2_GC_PERCENT=52.381
11031
PRIMER_LEFT_2_SELF_ANY=4.00
11032
PRIMER_RIGHT_2_SELF_ANY=4.00
11033
PRIMER_LEFT_2_SELF_END=2.00
11034
PRIMER_RIGHT_2_SELF_END=1.00
11035
PRIMER_LEFT_2_END_STABILITY=6.7000
11036
PRIMER_RIGHT_2_END_STABILITY=6.3000
11037
PRIMER_PAIR_2_COMPL_ANY=4.00
11038
PRIMER_PAIR_2_COMPL_END=0.00
11039
PRIMER_PRODUCT_SIZE_2=165
11040
PRIMER_PAIR_PENALTY_3=4.4612
11041
PRIMER_LEFT_3_PENALTY=3.320295
11042
PRIMER_RIGHT_3_PENALTY=1.140954
11043
PRIMER_LEFT_3_SEQUENCE=GGCGGAATAACTTCTAGTGTTCA
11044
PRIMER_RIGHT_3_SEQUENCE=TGACCTTTCACTCTGCCTCAT
11045
PRIMER_LEFT_3=161,23
11046
PRIMER_RIGHT_3=328,21
11047
PRIMER_LEFT_3_TM=59.680
11048
PRIMER_RIGHT_3_TM=59.859
11049
PRIMER_LEFT_3_GC_PERCENT=43.478
11050
PRIMER_RIGHT_3_GC_PERCENT=47.619
11051
PRIMER_LEFT_3_SELF_ANY=4.00
11052
PRIMER_RIGHT_3_SELF_ANY=3.00
11053
PRIMER_LEFT_3_SELF_END=2.00
11054
PRIMER_RIGHT_3_SELF_END=2.00
11055
PRIMER_LEFT_3_END_STABILITY=6.7000
11056
PRIMER_RIGHT_3_END_STABILITY=6.6000
11057
PRIMER_PAIR_3_COMPL_ANY=4.00
11058
PRIMER_PAIR_3_COMPL_END=1.00
11059
PRIMER_PRODUCT_SIZE_3=168
11060
PRIMER_PAIR_PENALTY_4=4.4612
11061
PRIMER_LEFT_4_PENALTY=3.320295
11062
PRIMER_RIGHT_4_PENALTY=1.140954
11063
PRIMER_LEFT_4_SEQUENCE=GGCGGAATAACTTCTAGTGTTCA
11064
PRIMER_RIGHT_4_SEQUENCE=GACCTTTCACTCTGCCTCATG
11065
PRIMER_LEFT_4=161,23
11066
PRIMER_RIGHT_4=327,21
11067
PRIMER_LEFT_4_TM=59.680
11068
PRIMER_RIGHT_4_TM=59.859
11069
PRIMER_LEFT_4_GC_PERCENT=43.478
11070
PRIMER_RIGHT_4_GC_PERCENT=52.381
11071
PRIMER_LEFT_4_SELF_ANY=4.00
11072
PRIMER_RIGHT_4_SELF_ANY=4.00
11073
PRIMER_LEFT_4_SELF_END=2.00
11074
PRIMER_RIGHT_4_SELF_END=4.00
11075
PRIMER_LEFT_4_END_STABILITY=6.7000
11076
PRIMER_RIGHT_4_END_STABILITY=6.9000
11077
PRIMER_PAIR_4_COMPL_ANY=4.00
11078
PRIMER_PAIR_4_COMPL_END=2.00
11079
PRIMER_PRODUCT_SIZE_4=167
11080
=
11081
MARKER_NAME=35.RHAP69FD11.seq
11082
SEQUENCE=NNNNNNNNNNNNNNNNNNNTTGCTCTTACATGTCTGACCTGCTTGTCCTGGTGGTTAGAACCACTTCACATTGTTATCTTACAGTTTTATTGATGGGTTGCCCATTTGCTCATTCTTTGGGACAGAAAGACTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCCCCTCTTCCTCACTGGCTGGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
11083
INCLUDED_REGION=19,173
11084
TARGET=133,34,CA
11085
PRIMER_COMMENT=CL is TGTCTGACCTGCTTGTCCTG
11086
PRIMER_COMMENT=CR is ACCCAGCCAGTGAGGAAGAG
11087
PRIMER_PAIR_PENALTY=1.7926
11088
PRIMER_LEFT_PENALTY=0.022009
11089
PRIMER_RIGHT_PENALTY=1.770544
11090
PRIMER_LEFT_SEQUENCE=TGTCTGACCTGCTTGTCCTG
11091
PRIMER_RIGHT_SEQUENCE=ACCCAGCCAGTGAGGAAGAG
11092
PRIMER_LEFT=30,20
11093
PRIMER_RIGHT=191,20
11094
PRIMER_LEFT_TM=60.022
11095
PRIMER_RIGHT_TM=61.771
11096
PRIMER_LEFT_GC_PERCENT=55.000
11097
PRIMER_RIGHT_GC_PERCENT=60.000
11098
PRIMER_LEFT_SELF_ANY=5.00
11099
PRIMER_RIGHT_SELF_ANY=3.00
11100
PRIMER_LEFT_SELF_END=1.00
11101
PRIMER_RIGHT_SELF_END=0.00
11102
PRIMER_LEFT_END_STABILITY=8.2000
11103
PRIMER_RIGHT_END_STABILITY=6.7000
11104
PRIMER_PAIR_COMPL_ANY=4.00
11105
PRIMER_PAIR_COMPL_END=3.00
11106
PRIMER_PRODUCT_SIZE=162
11107
PRIMER_PAIR_PENALTY_1=1.8340
11108
PRIMER_LEFT_1_PENALTY=0.022009
11109
PRIMER_RIGHT_1_PENALTY=1.811998
11110
PRIMER_LEFT_1_SEQUENCE=TGTCTGACCTGCTTGTCCTG
11111
PRIMER_RIGHT_1_SEQUENCE=ACCCAGCCAGTGAGGAAGA
11112
PRIMER_LEFT_1=30,20
11113
PRIMER_RIGHT_1=191,19
11114
PRIMER_LEFT_1_TM=60.022
11115
PRIMER_RIGHT_1_TM=60.812
11116
PRIMER_LEFT_1_GC_PERCENT=55.000
11117
PRIMER_RIGHT_1_GC_PERCENT=57.895
11118
PRIMER_LEFT_1_SELF_ANY=5.00
11119
PRIMER_RIGHT_1_SELF_ANY=3.00
11120
PRIMER_LEFT_1_SELF_END=1.00
11121
PRIMER_RIGHT_1_SELF_END=0.00
11122
PRIMER_LEFT_1_END_STABILITY=8.2000
11123
PRIMER_RIGHT_1_END_STABILITY=6.7000
11124
PRIMER_PAIR_1_COMPL_ANY=4.00
11125
PRIMER_PAIR_1_COMPL_END=2.00
11126
PRIMER_PRODUCT_SIZE_1=162
11127
PRIMER_PAIR_PENALTY_2=1.9713
11128
PRIMER_LEFT_2_PENALTY=0.022009
11129
PRIMER_RIGHT_2_PENALTY=1.949291
11130
PRIMER_LEFT_2_SEQUENCE=TGTCTGACCTGCTTGTCCTG
11131
PRIMER_RIGHT_2_SEQUENCE=CAGCCAGTGAGGAAGAGGG
11132
PRIMER_LEFT_2=30,20
11133
PRIMER_RIGHT_2=188,19
11134
PRIMER_LEFT_2_TM=60.022
11135
PRIMER_RIGHT_2_TM=60.949
11136
PRIMER_LEFT_2_GC_PERCENT=55.000
11137
PRIMER_RIGHT_2_GC_PERCENT=63.158
11138
PRIMER_LEFT_2_SELF_ANY=5.00
11139
PRIMER_RIGHT_2_SELF_ANY=3.00
11140
PRIMER_LEFT_2_SELF_END=1.00
11141
PRIMER_RIGHT_2_SELF_END=0.00
11142
PRIMER_LEFT_2_END_STABILITY=8.2000
11143
PRIMER_RIGHT_2_END_STABILITY=9.4000
11144
PRIMER_PAIR_2_COMPL_ANY=4.00
11145
PRIMER_PAIR_2_COMPL_END=3.00
11146
PRIMER_PRODUCT_SIZE_2=159
11147
PRIMER_PAIR_PENALTY_3=1.9713
11148
PRIMER_LEFT_3_PENALTY=0.022009
11149
PRIMER_RIGHT_3_PENALTY=1.949291
11150
PRIMER_LEFT_3_SEQUENCE=TGTCTGACCTGCTTGTCCTG
11151
PRIMER_RIGHT_3_SEQUENCE=CCCAGCCAGTGAGGAAGAG
11152
PRIMER_LEFT_3=30,20
11153
PRIMER_RIGHT_3=190,19
11154
PRIMER_LEFT_3_TM=60.022
11155
PRIMER_RIGHT_3_TM=60.949
11156
PRIMER_LEFT_3_GC_PERCENT=55.000
11157
PRIMER_RIGHT_3_GC_PERCENT=63.158
11158
PRIMER_LEFT_3_SELF_ANY=5.00
11159
PRIMER_RIGHT_3_SELF_ANY=3.00
11160
PRIMER_LEFT_3_SELF_END=1.00
11161
PRIMER_RIGHT_3_SELF_END=0.00
11162
PRIMER_LEFT_3_END_STABILITY=8.2000
11163
PRIMER_RIGHT_3_END_STABILITY=6.7000
11164
PRIMER_PAIR_3_COMPL_ANY=4.00
11165
PRIMER_PAIR_3_COMPL_END=3.00
11166
PRIMER_PRODUCT_SIZE_3=161
11167
PRIMER_PAIR_PENALTY_4=1.9713
11168
PRIMER_LEFT_4_PENALTY=0.022009
11169
PRIMER_RIGHT_4_PENALTY=1.949291
11170
PRIMER_LEFT_4_SEQUENCE=TGTCTGACCTGCTTGTCCTG
11171
PRIMER_RIGHT_4_SEQUENCE=CCAGCCAGTGAGGAAGAGG
11172
PRIMER_LEFT_4=30,20
11173
PRIMER_RIGHT_4=189,19
11174
PRIMER_LEFT_4_TM=60.022
11175
PRIMER_RIGHT_4_TM=60.949
11176
PRIMER_LEFT_4_GC_PERCENT=55.000
11177
PRIMER_RIGHT_4_GC_PERCENT=63.158
11178
PRIMER_LEFT_4_SELF_ANY=5.00
11179
PRIMER_RIGHT_4_SELF_ANY=3.00
11180
PRIMER_LEFT_4_SELF_END=1.00
11181
PRIMER_RIGHT_4_SELF_END=0.00
11182
PRIMER_LEFT_4_END_STABILITY=8.2000
11183
PRIMER_RIGHT_4_END_STABILITY=7.9000
11184
PRIMER_PAIR_4_COMPL_ANY=4.00
11185
PRIMER_PAIR_4_COMPL_END=4.00
11186
PRIMER_PRODUCT_SIZE_4=160
11187
=
11188
MARKER_NAME=39.RHAP69FE6.seq
11189
SEQUENCE=CNCTNNNNNNNNNNNNNNNNNCTCTGNACACATTCGGACACANATTNTTNACACACNTTTTCTGTGACACACATACNCAGNNAGATACACACNTAACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCACNGACTGTCAANNNNNNNNNNNNNNNNNNNNNNCTATACNGACGNNNNNNNNNNNNNNCTACAAACGGGACATAAAGCGGGACACGATGANAGGCTTTTCCNCNCACACNAAGTGATANCNNACAGCNTCNCACAGACANGNNNNCGNAGTGNTTGANNCNGACGCGCNCTCNTNANNGGAGGCACGGTCTAGNNNGCGCAGGTCTNGAAGGTCGGNTTAGNGNTGNNNNTANNGNNGCNCTTACNAACGCNGANTCATANTNTNTCAGANNNNGAGGGTNTNTTATNTAAAGNNTCAANANAAANAA
11190
INCLUDED_REGION=0,442
11191
TARGET=5,16,CA
11192
TARGET=99,32,AC
11193
TARGET=145,22,AC
11194
TARGET=178,14,CA
11195
PRIMER_COMMENT=CL is
11196
PRIMER_COMMENT=CR is
11197
=
11198
MARKER_NAME=40.RHAP69FF10.seq
11199
SEQUENCE=NNNNNNNNNNNNNNNNNNNACTNNTAAAAAATTGTCTTCAATATCTTTTTAAACCAATTAAATTTTTTAATTAAAATATAATTACATCACATCCTTTTTCCCTTTTGTCCCTCCAATCCCTCCCATCCTTTTCCATCACTTTCTTCCAAATTGATGGCCTCGTTTTCTTTGATGATTATTGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCACGCATGCACGCGCGCGCGCACGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTCAACCTTCTATATCCATTTAGTGCTGCTTGTATATGTTTTCACAGTAGAGCACTTTGTATTGGATAACCAATTAGGTTGCTCATCCCTG
11200
INCLUDED_REGION=19,361
11201
TARGET=183,40,AC
11202
TARGET=248,40,AC
11203
PRIMER_COMMENT=CL is CAAATTGATGGCCTCGTTTT
11204
PRIMER_COMMENT=CR is GCAGCACTAAATGGATATAGAAGG
11205
PRIMER_PAIR_PENALTY=5.6106
11206
PRIMER_LEFT_PENALTY=0.061961
11207
PRIMER_RIGHT_PENALTY=5.548624
11208
PRIMER_LEFT_SEQUENCE=CAAATTGATGGCCTCGTTTT
11209
PRIMER_RIGHT_SEQUENCE=GCAGCACTAAATGGATATAGAAGG
11210
PRIMER_LEFT=146,20
11211
PRIMER_RIGHT=317,24
11212
PRIMER_LEFT_TM=59.938
11213
PRIMER_RIGHT_TM=58.451
11214
PRIMER_LEFT_GC_PERCENT=40.000
11215
PRIMER_RIGHT_GC_PERCENT=41.667
11216
PRIMER_LEFT_SELF_ANY=6.00
11217
PRIMER_RIGHT_SELF_ANY=6.00
11218
PRIMER_LEFT_SELF_END=6.00
11219
PRIMER_RIGHT_SELF_END=0.00
11220
PRIMER_LEFT_END_STABILITY=7.0000
11221
PRIMER_RIGHT_END_STABILITY=8.2000
11222
PRIMER_PAIR_COMPL_ANY=3.00
11223
PRIMER_PAIR_COMPL_END=2.00
11224
PRIMER_PRODUCT_SIZE=172
11225
PRIMER_PAIR_PENALTY_1=5.7403
11226
PRIMER_LEFT_1_PENALTY=0.191680
11227
PRIMER_RIGHT_1_PENALTY=5.548624
11228
PRIMER_LEFT_1_SEQUENCE=GATGGCCTCGTTTTCTTTGA
11229
PRIMER_RIGHT_1_SEQUENCE=GCAGCACTAAATGGATATAGAAGG
11230
PRIMER_LEFT_1=152,20
11231
PRIMER_RIGHT_1=317,24
11232
PRIMER_LEFT_1_TM=60.192
11233
PRIMER_RIGHT_1_TM=58.451
11234
PRIMER_LEFT_1_GC_PERCENT=45.000
11235
PRIMER_RIGHT_1_GC_PERCENT=41.667
11236
PRIMER_LEFT_1_SELF_ANY=4.00
11237
PRIMER_RIGHT_1_SELF_ANY=6.00
11238
PRIMER_LEFT_1_SELF_END=1.00
11239
PRIMER_RIGHT_1_SELF_END=0.00
11240
PRIMER_LEFT_1_END_STABILITY=7.3000
11241
PRIMER_RIGHT_1_END_STABILITY=8.2000
11242
PRIMER_PAIR_1_COMPL_ANY=4.00
11243
PRIMER_PAIR_1_COMPL_END=1.00
11244
PRIMER_PRODUCT_SIZE_1=166
11245
PRIMER_PAIR_PENALTY_2=5.7403
11246
PRIMER_LEFT_2_PENALTY=0.191680
11247
PRIMER_RIGHT_2_PENALTY=5.548624
11248
PRIMER_LEFT_2_SEQUENCE=GGCCTCGTTTTCTTTGATGA
11249
PRIMER_RIGHT_2_SEQUENCE=GCAGCACTAAATGGATATAGAAGG
11250
PRIMER_LEFT_2=155,20
11251
PRIMER_RIGHT_2=317,24
11252
PRIMER_LEFT_2_TM=60.192
11253
PRIMER_RIGHT_2_TM=58.451
11254
PRIMER_LEFT_2_GC_PERCENT=45.000
11255
PRIMER_RIGHT_2_GC_PERCENT=41.667
11256
PRIMER_LEFT_2_SELF_ANY=4.00
11257
PRIMER_RIGHT_2_SELF_ANY=6.00
11258
PRIMER_LEFT_2_SELF_END=2.00
11259
PRIMER_RIGHT_2_SELF_END=0.00
11260
PRIMER_LEFT_2_END_STABILITY=6.6000
11261
PRIMER_RIGHT_2_END_STABILITY=8.2000
11262
PRIMER_PAIR_2_COMPL_ANY=4.00
11263
PRIMER_PAIR_2_COMPL_END=1.00
11264
PRIMER_PRODUCT_SIZE_2=163
11265
PRIMER_PAIR_PENALTY_3=5.7629
11266
PRIMER_LEFT_3_PENALTY=0.061961
11267
PRIMER_RIGHT_3_PENALTY=5.700947
11268
PRIMER_LEFT_3_SEQUENCE=CAAATTGATGGCCTCGTTTT
11269
PRIMER_RIGHT_3_SEQUENCE=AGCAGCACTAAATGGATATAGAAGG
11270
PRIMER_LEFT_3=146,20
11271
PRIMER_RIGHT_3=318,25
11272
PRIMER_LEFT_3_TM=59.938
11273
PRIMER_RIGHT_3_TM=59.299
11274
PRIMER_LEFT_3_GC_PERCENT=40.000
11275
PRIMER_RIGHT_3_GC_PERCENT=40.000
11276
PRIMER_LEFT_3_SELF_ANY=6.00
11277
PRIMER_RIGHT_3_SELF_ANY=6.00
11278
PRIMER_LEFT_3_SELF_END=6.00
11279
PRIMER_RIGHT_3_SELF_END=0.00
11280
PRIMER_LEFT_3_END_STABILITY=7.0000
11281
PRIMER_RIGHT_3_END_STABILITY=8.2000
11282
PRIMER_PAIR_3_COMPL_ANY=3.00
11283
PRIMER_PAIR_3_COMPL_END=2.00
11284
PRIMER_PRODUCT_SIZE_3=173
11285
PRIMER_PAIR_PENALTY_4=5.7666
11286
PRIMER_LEFT_4_PENALTY=0.191680
11287
PRIMER_RIGHT_4_PENALTY=5.574950
11288
PRIMER_LEFT_4_SEQUENCE=GGCCTCGTTTTCTTTGATGA
11289
PRIMER_RIGHT_4_SEQUENCE=CATATACAAGCAGCACTAAATGGA
11290
PRIMER_LEFT_4=155,20
11291
PRIMER_RIGHT_4=326,24
11292
PRIMER_LEFT_4_TM=60.192
11293
PRIMER_RIGHT_4_TM=58.425
11294
PRIMER_LEFT_4_GC_PERCENT=45.000
11295
PRIMER_RIGHT_4_GC_PERCENT=37.500
11296
PRIMER_LEFT_4_SELF_ANY=4.00
11297
PRIMER_RIGHT_4_SELF_ANY=4.00
11298
PRIMER_LEFT_4_SELF_END=2.00
11299
PRIMER_RIGHT_4_SELF_END=0.00
11300
PRIMER_LEFT_4_END_STABILITY=6.6000
11301
PRIMER_RIGHT_4_END_STABILITY=8.1000
11302
PRIMER_PAIR_4_COMPL_ANY=3.00
11303
PRIMER_PAIR_4_COMPL_END=1.00
11304
PRIMER_PRODUCT_SIZE_4=172
11305
=
11306
MARKER_NAME=40.RHAP69FF4.seq
11307
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCACGCACACATCTGCATACTNNNNNNNNNNNNNNNNNNNNNNNNGACATACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAATGGGCCCTTTGGCTTTTTTCTGATGACCTATCACCATCTCAAGTTCAAGACCCTGCACAGACCAGGTAATTTAAGAATCATCACCTTAAATTCAAGTTGCACCTTATGATTTAATTATTTCATGGAGATGGCTTTCTCCTTTCCAGTTTTATTCTCCATAAACTGAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
11308
INCLUDED_REGION=17,308
11309
TARGET=17,21,AC
11310
TARGET=60,24,CA
11311
TARGET=93,60,AC
11312
PRIMER_COMMENT=CL is GCACGCACACATCTGCATAC
11313
PRIMER_COMMENT=CR is TGCAGGGTCTTGAACTTGAG
11314
PRIMER_PAIR_PENALTY=2.3377
11315
PRIMER_LEFT_PENALTY=1.344726
11316
PRIMER_RIGHT_PENALTY=0.992992
11317
PRIMER_LEFT_SEQUENCE=GCACGCACACATCTGCATAC
11318
PRIMER_RIGHT_SEQUENCE=TGCAGGGTCTTGAACTTGAG
11319
PRIMER_LEFT=39,20
11320
PRIMER_RIGHT=213,20
11321
PRIMER_LEFT_TM=61.345
11322
PRIMER_RIGHT_TM=59.007
11323
PRIMER_LEFT_GC_PERCENT=55.000
11324
PRIMER_RIGHT_GC_PERCENT=50.000
11325
PRIMER_LEFT_SELF_ANY=4.00
11326
PRIMER_RIGHT_SELF_ANY=4.00
11327
PRIMER_LEFT_SELF_END=4.00
11328
PRIMER_RIGHT_SELF_END=2.00
11329
PRIMER_LEFT_END_STABILITY=5.6000
11330
PRIMER_RIGHT_END_STABILITY=7.0000
11331
PRIMER_PAIR_COMPL_ANY=5.00
11332
PRIMER_PAIR_COMPL_END=1.00
11333
PRIMER_PRODUCT_SIZE=175
11334
PRIMER_PAIR_PENALTY_1=2.4514
11335
PRIMER_LEFT_1_PENALTY=1.458379
11336
PRIMER_RIGHT_1_PENALTY=0.992992
11337
PRIMER_LEFT_1_SEQUENCE=GCACGCACACATCTGCATA
11338
PRIMER_RIGHT_1_SEQUENCE=TGCAGGGTCTTGAACTTGAG
11339
PRIMER_LEFT_1=39,19
11340
PRIMER_RIGHT_1=213,20
11341
PRIMER_LEFT_1_TM=60.458
11342
PRIMER_RIGHT_1_TM=59.007
11343
PRIMER_LEFT_1_GC_PERCENT=52.632
11344
PRIMER_RIGHT_1_GC_PERCENT=50.000
11345
PRIMER_LEFT_1_SELF_ANY=4.00
11346
PRIMER_RIGHT_1_SELF_ANY=4.00
11347
PRIMER_LEFT_1_SELF_END=4.00
11348
PRIMER_RIGHT_1_SELF_END=2.00
11349
PRIMER_LEFT_1_END_STABILITY=7.4000
11350
PRIMER_RIGHT_1_END_STABILITY=7.0000
11351
PRIMER_PAIR_1_COMPL_ANY=5.00
11352
PRIMER_PAIR_1_COMPL_END=1.00
11353
PRIMER_PRODUCT_SIZE_1=175
11354
PRIMER_PAIR_PENALTY_2=2.5359
11355
PRIMER_LEFT_2_PENALTY=1.344726
11356
PRIMER_RIGHT_2_PENALTY=1.191170
11357
PRIMER_LEFT_2_SEQUENCE=GCACGCACACATCTGCATAC
11358
PRIMER_RIGHT_2_SEQUENCE=CAGAAAAAAGCCAAAGGGC
11359
PRIMER_LEFT_2=39,20
11360
PRIMER_RIGHT_2=178,19
11361
PRIMER_LEFT_2_TM=61.345
11362
PRIMER_RIGHT_2_TM=59.809
11363
PRIMER_LEFT_2_GC_PERCENT=55.000
11364
PRIMER_RIGHT_2_GC_PERCENT=47.368
11365
PRIMER_LEFT_2_SELF_ANY=4.00
11366
PRIMER_RIGHT_2_SELF_ANY=3.00
11367
PRIMER_LEFT_2_SELF_END=4.00
11368
PRIMER_RIGHT_2_SELF_END=2.00
11369
PRIMER_LEFT_2_END_STABILITY=5.6000
11370
PRIMER_RIGHT_2_END_STABILITY=10.9000
11371
PRIMER_PAIR_2_COMPL_ANY=4.00
11372
PRIMER_PAIR_2_COMPL_END=0.00
11373
PRIMER_PRODUCT_SIZE_2=140
11374
PRIMER_PAIR_PENALTY_3=2.6495
11375
PRIMER_LEFT_3_PENALTY=1.458379
11376
PRIMER_RIGHT_3_PENALTY=1.191170
11377
PRIMER_LEFT_3_SEQUENCE=GCACGCACACATCTGCATA
11378
PRIMER_RIGHT_3_SEQUENCE=CAGAAAAAAGCCAAAGGGC
11379
PRIMER_LEFT_3=39,19
11380
PRIMER_RIGHT_3=178,19
11381
PRIMER_LEFT_3_TM=60.458
11382
PRIMER_RIGHT_3_TM=59.809
11383
PRIMER_LEFT_3_GC_PERCENT=52.632
11384
PRIMER_RIGHT_3_GC_PERCENT=47.368
11385
PRIMER_LEFT_3_SELF_ANY=4.00
11386
PRIMER_RIGHT_3_SELF_ANY=3.00
11387
PRIMER_LEFT_3_SELF_END=4.00
11388
PRIMER_RIGHT_3_SELF_END=2.00
11389
PRIMER_LEFT_3_END_STABILITY=7.4000
11390
PRIMER_RIGHT_3_END_STABILITY=10.9000
11391
PRIMER_PAIR_3_COMPL_ANY=4.00
11392
PRIMER_PAIR_3_COMPL_END=0.00
11393
PRIMER_PRODUCT_SIZE_3=140
11394
PRIMER_PAIR_PENALTY_4=2.6873
11395
PRIMER_LEFT_4_PENALTY=1.694261
11396
PRIMER_RIGHT_4_PENALTY=0.992992
11397
PRIMER_LEFT_4_SEQUENCE=CACGCACACATCTGCATACT
11398
PRIMER_RIGHT_4_SEQUENCE=TGCAGGGTCTTGAACTTGAG
11399
PRIMER_LEFT_4=40,20
11400
PRIMER_RIGHT_4=213,20
11401
PRIMER_LEFT_4_TM=58.306
11402
PRIMER_RIGHT_4_TM=59.007
11403
PRIMER_LEFT_4_GC_PERCENT=50.000
11404
PRIMER_RIGHT_4_GC_PERCENT=50.000
11405
PRIMER_LEFT_4_SELF_ANY=4.00
11406
PRIMER_RIGHT_4_SELF_ANY=4.00
11407
PRIMER_LEFT_4_SELF_END=2.00
11408
PRIMER_RIGHT_4_SELF_END=2.00
11409
PRIMER_LEFT_4_END_STABILITY=5.3000
11410
PRIMER_RIGHT_4_END_STABILITY=7.0000
11411
PRIMER_PAIR_4_COMPL_ANY=5.00
11412
PRIMER_PAIR_4_COMPL_END=2.00
11413
PRIMER_PRODUCT_SIZE_4=174
11414
=
11415
MARKER_NAME=41.RHAP69FF11.seq
11416
SEQUENCE=NNNNNNNNNNNNNNNNNNTAAGAGCTTAACAACTTATGGCAGCTTTTTAAAATCTGTCCACTTTTCTAAGTGCACTGTCTTCTTTGTCTCCCTTCTATCGTAGTTTTAAGCACATTTTCCTTCACACTTTGAGTTTTCNNNNNNNNNNNNNNNCATGACTCCTCTGCCATTATGTGACTGTTGTCCACTATGTGGCTGACCTCAATGTGGGGTTAACTCACATGACATGATTTTTTCCCCCTTTTCTCCCCTTCATCTTCCTTCTCTAGGCACCTGCTGAGATTTACAGCCTTGGGGATATTTTTTAAGCCTGAAAAGNNNNNNNNNNNNNNNNNNNNACAGNNNNNNNNNNNNNNNNNGNCANNNCGCTACNGNGNG
11417
INCLUDED_REGION=18,360
11418
TARGET=138,15,CT
11419
TARGET=318,20,CA
11420
TARGET=342,17,AC
11421
PRIMER_COMMENT=CL is GCACTGTCTTCTTTGTCTCCC
11422
PRIMER_COMMENT=CR is TGAGGTCAGCCACATAGTGG
11423
PRIMER_PAIR_PENALTY=2.3674
11424
PRIMER_LEFT_PENALTY=2.068459
11425
PRIMER_RIGHT_PENALTY=0.298942
11426
PRIMER_LEFT_SEQUENCE=GCACTGTCTTCTTTGTCTCCC
11427
PRIMER_RIGHT_SEQUENCE=TGAGGTCAGCCACATAGTGG
11428
PRIMER_LEFT=71,21
11429
PRIMER_RIGHT=203,20
11430
PRIMER_LEFT_TM=58.932
11431
PRIMER_RIGHT_TM=59.701
11432
PRIMER_LEFT_GC_PERCENT=52.381
11433
PRIMER_RIGHT_GC_PERCENT=55.000
11434
PRIMER_LEFT_SELF_ANY=3.00
11435
PRIMER_RIGHT_SELF_ANY=6.00
11436
PRIMER_LEFT_SELF_END=0.00
11437
PRIMER_RIGHT_SELF_END=6.00
11438
PRIMER_LEFT_END_STABILITY=9.4000
11439
PRIMER_RIGHT_END_STABILITY=7.9000
11440
PRIMER_PAIR_COMPL_ANY=4.00
11441
PRIMER_PAIR_COMPL_END=2.00
11442
PRIMER_PRODUCT_SIZE=133
11443
PRIMER_PAIR_PENALTY_1=2.3763
11444
PRIMER_LEFT_1_PENALTY=2.077340
11445
PRIMER_RIGHT_1_PENALTY=0.298942
11446
PRIMER_LEFT_1_SEQUENCE=GCACTGTCTTCTTTGTCTCCCT
11447
PRIMER_RIGHT_1_SEQUENCE=TGAGGTCAGCCACATAGTGG
11448
PRIMER_LEFT_1=71,22
11449
PRIMER_RIGHT_1=203,20
11450
PRIMER_LEFT_1_TM=59.923
11451
PRIMER_RIGHT_1_TM=59.701
11452
PRIMER_LEFT_1_GC_PERCENT=50.000
11453
PRIMER_RIGHT_1_GC_PERCENT=55.000
11454
PRIMER_LEFT_1_SELF_ANY=3.00
11455
PRIMER_RIGHT_1_SELF_ANY=6.00
11456
PRIMER_LEFT_1_SELF_END=0.00
11457
PRIMER_RIGHT_1_SELF_END=6.00
11458
PRIMER_LEFT_1_END_STABILITY=9.4000
11459
PRIMER_RIGHT_1_END_STABILITY=7.9000
11460
PRIMER_PAIR_1_COMPL_ANY=4.00
11461
PRIMER_PAIR_1_COMPL_END=2.00
11462
PRIMER_PRODUCT_SIZE_1=133
11463
PRIMER_PAIR_PENALTY_2=2.4942
11464
PRIMER_LEFT_2_PENALTY=2.345718
11465
PRIMER_RIGHT_2_PENALTY=0.148509
11466
PRIMER_LEFT_2_SEQUENCE=GCACATTTTCCTTCACACTTTG
11467
PRIMER_RIGHT_2_SEQUENCE=CAGCAGGTGCCTAGAGAAGG
11468
PRIMER_LEFT_2=109,22
11469
PRIMER_RIGHT_2=278,20
11470
PRIMER_LEFT_2_TM=59.654
11471
PRIMER_RIGHT_2_TM=60.149
11472
PRIMER_LEFT_2_GC_PERCENT=40.909
11473
PRIMER_RIGHT_2_GC_PERCENT=60.000
11474
PRIMER_LEFT_2_SELF_ANY=3.00
11475
PRIMER_RIGHT_2_SELF_ANY=4.00
11476
PRIMER_LEFT_2_SELF_END=3.00
11477
PRIMER_RIGHT_2_SELF_END=1.00
11478
PRIMER_LEFT_2_END_STABILITY=7.3000
11479
PRIMER_RIGHT_2_END_STABILITY=8.2000
11480
PRIMER_PAIR_2_COMPL_ANY=5.00
11481
PRIMER_PAIR_2_COMPL_END=1.00
11482
PRIMER_PRODUCT_SIZE_2=170
11483
PRIMER_PAIR_PENALTY_3=2.5173
11484
PRIMER_LEFT_3_PENALTY=2.068459
11485
PRIMER_RIGHT_3_PENALTY=0.448810
11486
PRIMER_LEFT_3_SEQUENCE=GCACTGTCTTCTTTGTCTCCC
11487
PRIMER_RIGHT_3_SEQUENCE=CACATTGAGGTCAGCCACAT
11488
PRIMER_LEFT_3=71,21
11489
PRIMER_RIGHT_3=208,20
11490
PRIMER_LEFT_3_TM=58.932
11491
PRIMER_RIGHT_3_TM=59.551
11492
PRIMER_LEFT_3_GC_PERCENT=52.381
11493
PRIMER_RIGHT_3_GC_PERCENT=50.000
11494
PRIMER_LEFT_3_SELF_ANY=3.00
11495
PRIMER_RIGHT_3_SELF_ANY=4.00
11496
PRIMER_LEFT_3_SELF_END=0.00
11497
PRIMER_RIGHT_3_SELF_END=2.00
11498
PRIMER_LEFT_3_END_STABILITY=9.4000
11499
PRIMER_RIGHT_3_END_STABILITY=6.6000
11500
PRIMER_PAIR_3_COMPL_ANY=5.00
11501
PRIMER_PAIR_3_COMPL_END=1.00
11502
PRIMER_PRODUCT_SIZE_3=138
11503
PRIMER_PAIR_PENALTY_4=2.5173
11504
PRIMER_LEFT_4_PENALTY=2.068459
11505
PRIMER_RIGHT_4_PENALTY=0.448810
11506
PRIMER_LEFT_4_SEQUENCE=GCACTGTCTTCTTTGTCTCCC
11507
PRIMER_RIGHT_4_SEQUENCE=GGTCAGCCACATAGTGGACA
11508
PRIMER_LEFT_4=71,21
11509
PRIMER_RIGHT_4=200,20
11510
PRIMER_LEFT_4_TM=58.932
11511
PRIMER_RIGHT_4_TM=59.551
11512
PRIMER_LEFT_4_GC_PERCENT=52.381
11513
PRIMER_RIGHT_4_GC_PERCENT=55.000
11514
PRIMER_LEFT_4_SELF_ANY=3.00
11515
PRIMER_RIGHT_4_SELF_ANY=6.00
11516
PRIMER_LEFT_4_SELF_END=0.00
11517
PRIMER_RIGHT_4_SELF_END=2.00
11518
PRIMER_LEFT_4_END_STABILITY=9.4000
11519
PRIMER_RIGHT_4_END_STABILITY=7.9000
11520
PRIMER_PAIR_4_COMPL_ANY=6.00
11521
PRIMER_PAIR_4_COMPL_END=3.00
11522
PRIMER_PRODUCT_SIZE_4=130
11523
=
11524
MARKER_NAME=42.RHAP69FF6.seq
11525
SEQUENCE=CACTNNNNNNNNNNNNNNNNCACTGNCACACGNTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCAAACCAGACAGAGAGTTGACACATTCACAAGTTCAAGACGCAAAGTGTTCTTTTCTTCGAATTACACTTCCTTGAGTTTCGCCTGGCCTTGGGTTGTTTTGAAGTTGGCAAGTCTCCTCAAAGCTGCTTTTGAGAGCCACCGTGTTCATTAGCTGGTTCACAGCTTCCTCCTACCACTTAGCTTGCCTGGAACCCTGCTGTGCAAACAGGCCTGGTGAGCTGGGATGTCTGGCACGTGCAAGGCCCAGGTTCCACCCTGGCACAGTGAGGAGGAAGCAGGCCTTGTGTGTGGACACCCAGTCCCTGTGGCACTTAGAGCCTACCNNNNNNNNNNNNNNNNNNNNNNNNNN
11526
INCLUDED_REGION=0,428
11527
TARGET=4,16,AC
11528
TARGET=35,40,AC
11529
TARGET=402,26,AG
11530
PRIMER_COMMENT=CL is
11531
PRIMER_COMMENT=CR is
11532
=
11533
MARKER_NAME=44.RHAP69FG5.seq
11534
SEQUENCE=AANTNTATNNTTGNNAGGNNNANGNANGGAGNNNNNNNGGANNGNNNNNTANNGTNNAANNNGNTNGGGNNNTNGGNNNNANNNACNNNNTCTNAANNTCTATTNANTNCCACNTNNTGGGGNNAACCATNCANTNNCAGNTGTGCTTNNGAGGGTGGNNTTCTGATTTNAAAACCAANCAACANNAANNNATTAGANANNNNNNNTNNCACAACACNCATNANNCNNNNNNNNNNNNNNNNNNNACNCNNAAACTCCANTCACNCANAAATTATTCTTCTTCNCCAGCNANTTCATGAAANTTTTCTTCAAGAACCACATTACTTTGGGCACAAAAATAAGGCTCNTCAGGATGACAAAGAAAAATTGNACTAGGNACTGGNTTAAAAGAANAGCATAATTCAAACAATATAATGNTGGGNAAAANTGAATGGGCTGACAGATAGAAGAACTTGATCANCANGGAACANNATTTNAACTTCAAGTTAATNAAAAACNC
11535
INCLUDED_REGION=0,499
11536
TARGET=228,14,CA
11537
PRIMER_COMMENT=CL is
11538
PRIMER_COMMENT=CR is
11539
=
11540
MARKER_NAME=01.RHAP131FA1.seq
11541
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCCNTTAAGGATTACAANACTGAACCACCAACCAANNAGCATGCATNGGNNTGACAANGNCNNNNNNNNNNNNNNNACACNCTCGNCNCNCTCNCACNCACNCNCTCTCNNNCNTCTNTATNTAGNGNGGTATANTTGNGTCNCTAAANNTAGAATGTTNNNTNNGNNATNTTTNNANANGGGGGNNGNTNACCTACANGNNCNCTNTTNGCTAACNNTTTTGTTATTGCCANTCAACTNNAANNTTTGGGNGNCNNNNTTNTTGNTCTAGANTTTTNAATGTNNANAAAAANANNNAANNTNNTAAGANTNNNNTNNGGTNNNNTNNTAAAAGGGNGTTTTTCNNCNAATAANANNGGGGNAANANNNNAGGGGGAGNNNNTNANAAAANNNGGGGNTNNTTGNANNNNNNAATANNNATAGNGTTNAANNNNAATNNNNTTCAATTNNNAAATGNNAAGNGTTNNAAAAAAANTGTTNNTNNNTTNNTTNAAANTGATGANTNTGNNTANNACNAANTTTCTNNGNNGAGAANNCCGAAGCCTCCNAAANNCTNTNAATTNGNNNAANCAANAANNNGNNGGCNNAAANAAANNATTGTTNNNCCCNNNNAAAATTNCTCNNCCNNNAANNANGACGNNNNNANNATNNNNGGNNANATNCNNTGGGGNAGCNTNAATTNNATGANNTTNNNNNACAAANNAATTNGANNANNCTA
11542
INCLUDED_REGION=23,717
11543
TARGET=84,14,CA
11544
PRIMER_COMMENT=CL is
11545
PRIMER_COMMENT=CR is
11546
=
11547
MARKER_NAME=01.RHAP131FA7.seq
11548
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCAGTATAAGTATGTCTGGAGATCTTTTAGAAATGAGGGGTAAATTCTGAGGGCAGGAAGGTTTTAGGGGAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAACTGCCTGCAAATTGGTATAGGCTGAGAAATTCAATTAATCGTATTCATCAATTTTTCACTCTGGGGGGGGAAATGAGGCAGAGATGTGGAATTCTTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
11549
INCLUDED_REGION=23,224
11550
TARGET=95,50,GT
11551
PRIMER_COMMENT=CL is GAGGGCAGGAAGGTTTTAGG
11552
PRIMER_COMMENT=CR is CCCCCAGAGTGAAAAATTGA
11553
PRIMER_PAIR_PENALTY=0.1644
11554
PRIMER_LEFT_PENALTY=0.067516
11555
PRIMER_RIGHT_PENALTY=0.096855
11556
PRIMER_LEFT_SEQUENCE=GAGGGCAGGAAGGTTTTAGG
11557
PRIMER_RIGHT_SEQUENCE=CCCCCAGAGTGAAAAATTGA
11558
PRIMER_LEFT=70,20
11559
PRIMER_RIGHT=215,20
11560
PRIMER_LEFT_TM=60.068
11561
PRIMER_RIGHT_TM=59.903
11562
PRIMER_LEFT_GC_PERCENT=55.000
11563
PRIMER_RIGHT_GC_PERCENT=45.000
11564
PRIMER_LEFT_SELF_ANY=2.00
11565
PRIMER_RIGHT_SELF_ANY=4.00
11566
PRIMER_LEFT_SELF_END=0.00
11567
PRIMER_RIGHT_SELF_END=1.00
11568
PRIMER_LEFT_END_STABILITY=7.5000
11569
PRIMER_RIGHT_END_STABILITY=6.9000
11570
PRIMER_PAIR_COMPL_ANY=4.00
11571
PRIMER_PAIR_COMPL_END=0.00
11572
PRIMER_PRODUCT_SIZE=146
11573
PRIMER_PAIR_PENALTY_1=0.1707
11574
PRIMER_LEFT_1_PENALTY=0.073866
11575
PRIMER_RIGHT_1_PENALTY=0.096855
11576
PRIMER_LEFT_1_SEQUENCE=AATTCTGAGGGCAGGAAGGT
11577
PRIMER_RIGHT_1_SEQUENCE=CCCCCAGAGTGAAAAATTGA
11578
PRIMER_LEFT_1=64,20
11579
PRIMER_RIGHT_1=215,20
11580
PRIMER_LEFT_1_TM=60.074
11581
PRIMER_RIGHT_1_TM=59.903
11582
PRIMER_LEFT_1_GC_PERCENT=50.000
11583
PRIMER_RIGHT_1_GC_PERCENT=45.000
11584
PRIMER_LEFT_1_SELF_ANY=4.00
11585
PRIMER_RIGHT_1_SELF_ANY=4.00
11586
PRIMER_LEFT_1_SELF_END=0.00
11587
PRIMER_RIGHT_1_SELF_END=1.00
11588
PRIMER_LEFT_1_END_STABILITY=7.9000
11589
PRIMER_RIGHT_1_END_STABILITY=6.9000
11590
PRIMER_PAIR_1_COMPL_ANY=6.00
11591
PRIMER_PAIR_1_COMPL_END=1.00
11592
PRIMER_PRODUCT_SIZE_1=152
11593
PRIMER_PAIR_PENALTY_2=0.1707
11594
PRIMER_LEFT_2_PENALTY=0.073866
11595
PRIMER_RIGHT_2_PENALTY=0.096855
11596
PRIMER_LEFT_2_SEQUENCE=ATTCTGAGGGCAGGAAGGTT
11597
PRIMER_RIGHT_2_SEQUENCE=CCCCCAGAGTGAAAAATTGA
11598
PRIMER_LEFT_2=65,20
11599
PRIMER_RIGHT_2=215,20
11600
PRIMER_LEFT_2_TM=60.074
11601
PRIMER_RIGHT_2_TM=59.903
11602
PRIMER_LEFT_2_GC_PERCENT=50.000
11603
PRIMER_RIGHT_2_GC_PERCENT=45.000
11604
PRIMER_LEFT_2_SELF_ANY=3.00
11605
PRIMER_RIGHT_2_SELF_ANY=4.00
11606
PRIMER_LEFT_2_SELF_END=2.00
11607
PRIMER_RIGHT_2_SELF_END=1.00
11608
PRIMER_LEFT_2_END_STABILITY=7.9000
11609
PRIMER_RIGHT_2_END_STABILITY=6.9000
11610
PRIMER_PAIR_2_COMPL_ANY=6.00
11611
PRIMER_PAIR_2_COMPL_END=1.00
11612
PRIMER_PRODUCT_SIZE_2=151
11613
PRIMER_PAIR_PENALTY_3=0.1937
11614
PRIMER_LEFT_3_PENALTY=0.096820
11615
PRIMER_RIGHT_3_PENALTY=0.096855
11616
PRIMER_LEFT_3_SEQUENCE=GAGGGGTAAATTCTGAGGGC
11617
PRIMER_RIGHT_3_SEQUENCE=CCCCCAGAGTGAAAAATTGA
11618
PRIMER_LEFT_3=56,20
11619
PRIMER_RIGHT_3=215,20
11620
PRIMER_LEFT_3_TM=59.903
11621
PRIMER_RIGHT_3_TM=59.903
11622
PRIMER_LEFT_3_GC_PERCENT=55.000
11623
PRIMER_RIGHT_3_GC_PERCENT=45.000
11624
PRIMER_LEFT_3_SELF_ANY=4.00
11625
PRIMER_RIGHT_3_SELF_ANY=4.00
11626
PRIMER_LEFT_3_SELF_END=2.00
11627
PRIMER_RIGHT_3_SELF_END=1.00
11628
PRIMER_LEFT_3_END_STABILITY=10.9000
11629
PRIMER_RIGHT_3_END_STABILITY=6.9000
11630
PRIMER_PAIR_3_COMPL_ANY=6.00
11631
PRIMER_PAIR_3_COMPL_END=0.00
11632
PRIMER_PRODUCT_SIZE_3=160
11633
PRIMER_PAIR_PENALTY_4=0.5197
11634
PRIMER_LEFT_4_PENALTY=0.422888
11635
PRIMER_RIGHT_4_PENALTY=0.096855
11636
PRIMER_LEFT_4_SEQUENCE=GCAGGAAGGTTTTAGGGGAA
11637
PRIMER_RIGHT_4_SEQUENCE=CCCCCAGAGTGAAAAATTGA
11638
PRIMER_LEFT_4=74,20
11639
PRIMER_RIGHT_4=215,20
11640
PRIMER_LEFT_4_TM=60.423
11641
PRIMER_RIGHT_4_TM=59.903
11642
PRIMER_LEFT_4_GC_PERCENT=50.000
11643
PRIMER_RIGHT_4_GC_PERCENT=45.000
11644
PRIMER_LEFT_4_SELF_ANY=2.00
11645
PRIMER_RIGHT_4_SELF_ANY=4.00
11646
PRIMER_LEFT_4_SELF_END=0.00
11647
PRIMER_RIGHT_4_SELF_END=1.00
11648
PRIMER_LEFT_4_END_STABILITY=9.7000
11649
PRIMER_RIGHT_4_END_STABILITY=6.9000
11650
PRIMER_PAIR_4_COMPL_ANY=4.00
11651
PRIMER_PAIR_4_COMPL_END=0.00
11652
PRIMER_PRODUCT_SIZE_4=142
11653
=
11654
MARKER_NAME=02.RHAP131FA8.seq
11655
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNCCAGTATAAGTATGTCTGGANTAATAATAGCAAAAAGTATAAAACCATGAGGGCAGGAAGGGTGGCTCCACTGGGATTACCTGCTGGAACCTCCTCCCTTTCCACAGGAACCTGAGCTTGTGACAAACTAATAAAATATGGAATTACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGCCAGAAGAACAGGGAGATCTTGGAGGACCCATTCCAAGCATTGTTTATTCATTCATGTGGACACCGTGACTTGGGGGCTGGAGAGAGGGTGCAGCAGTTGAGAATAGACAACTCTTCCTGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
11656
INCLUDED_REGION=22,336
11657
TARGET=170,61,AC
11658
PRIMER_COMMENT=CL is TTCCACAGGAACCTGAGCTT
11659
PRIMER_COMMENT=CR is AACAATGCTTGGAATGGGTC
11660
PRIMER_PAIR_PENALTY=0.3594
11661
PRIMER_LEFT_PENALTY=0.156061
11662
PRIMER_RIGHT_PENALTY=0.203367
11663
PRIMER_LEFT_SEQUENCE=TTCCACAGGAACCTGAGCTT
11664
PRIMER_RIGHT_SEQUENCE=AACAATGCTTGGAATGGGTC
11665
PRIMER_LEFT=121,20
11666
PRIMER_RIGHT=279,20
11667
PRIMER_LEFT_TM=59.844
11668
PRIMER_RIGHT_TM=59.797
11669
PRIMER_LEFT_GC_PERCENT=50.000
11670
PRIMER_RIGHT_GC_PERCENT=45.000
11671
PRIMER_LEFT_SELF_ANY=6.00
11672
PRIMER_RIGHT_SELF_ANY=4.00
11673
PRIMER_LEFT_SELF_END=5.00
11674
PRIMER_RIGHT_SELF_END=1.00
11675
PRIMER_LEFT_END_STABILITY=8.2000
11676
PRIMER_RIGHT_END_STABILITY=9.1000
11677
PRIMER_PAIR_COMPL_ANY=5.00
11678
PRIMER_PAIR_COMPL_END=0.00
11679
PRIMER_PRODUCT_SIZE=159
11680
PRIMER_PAIR_PENALTY_1=0.3594
11681
PRIMER_LEFT_1_PENALTY=0.156061
11682
PRIMER_RIGHT_1_PENALTY=0.203367
11683
PRIMER_LEFT_1_SEQUENCE=TTTCCACAGGAACCTGAGCT
11684
PRIMER_RIGHT_1_SEQUENCE=AACAATGCTTGGAATGGGTC
11685
PRIMER_LEFT_1=120,20
11686
PRIMER_RIGHT_1=279,20
11687
PRIMER_LEFT_1_TM=59.844
11688
PRIMER_RIGHT_1_TM=59.797
11689
PRIMER_LEFT_1_GC_PERCENT=50.000
11690
PRIMER_RIGHT_1_GC_PERCENT=45.000
11691
PRIMER_LEFT_1_SELF_ANY=6.00
11692
PRIMER_RIGHT_1_SELF_ANY=4.00
11693
PRIMER_LEFT_1_SELF_END=4.00
11694
PRIMER_RIGHT_1_SELF_END=1.00
11695
PRIMER_LEFT_1_END_STABILITY=7.9000
11696
PRIMER_RIGHT_1_END_STABILITY=9.1000
11697
PRIMER_PAIR_1_COMPL_ANY=5.00
11698
PRIMER_PAIR_1_COMPL_END=1.00
11699
PRIMER_PRODUCT_SIZE_1=160
11700
PRIMER_PAIR_PENALTY_2=0.3594
11701
PRIMER_LEFT_2_PENALTY=0.156061
11702
PRIMER_RIGHT_2_PENALTY=0.203367
11703
PRIMER_LEFT_2_SEQUENCE=CTTTCCACAGGAACCTGAGC
11704
PRIMER_RIGHT_2_SEQUENCE=AACAATGCTTGGAATGGGTC
11705
PRIMER_LEFT_2=119,20
11706
PRIMER_RIGHT_2=279,20
11707
PRIMER_LEFT_2_TM=59.844
11708
PRIMER_RIGHT_2_TM=59.797
11709
PRIMER_LEFT_2_GC_PERCENT=55.000
11710
PRIMER_RIGHT_2_GC_PERCENT=45.000
11711
PRIMER_LEFT_2_SELF_ANY=6.00
11712
PRIMER_RIGHT_2_SELF_ANY=4.00
11713
PRIMER_LEFT_2_SELF_END=4.00
11714
PRIMER_RIGHT_2_SELF_END=1.00
11715
PRIMER_LEFT_2_END_STABILITY=8.2000
11716
PRIMER_RIGHT_2_END_STABILITY=9.1000
11717
PRIMER_PAIR_2_COMPL_ANY=5.00
11718
PRIMER_PAIR_2_COMPL_END=2.00
11719
PRIMER_PRODUCT_SIZE_2=161
11720
PRIMER_PAIR_PENALTY_3=0.4342
11721
PRIMER_LEFT_3_PENALTY=0.156061
11722
PRIMER_RIGHT_3_PENALTY=0.278164
11723
PRIMER_LEFT_3_SEQUENCE=CTTTCCACAGGAACCTGAGC
11724
PRIMER_RIGHT_3_SEQUENCE=ATGGGTCCTCCAAGATCTCC
11725
PRIMER_LEFT_3=119,20
11726
PRIMER_RIGHT_3=266,20
11727
PRIMER_LEFT_3_TM=59.844
11728
PRIMER_RIGHT_3_TM=60.278
11729
PRIMER_LEFT_3_GC_PERCENT=55.000
11730
PRIMER_RIGHT_3_GC_PERCENT=55.000
11731
PRIMER_LEFT_3_SELF_ANY=6.00
11732
PRIMER_RIGHT_3_SELF_ANY=6.00
11733
PRIMER_LEFT_3_SELF_END=4.00
11734
PRIMER_RIGHT_3_SELF_END=2.00
11735
PRIMER_LEFT_3_END_STABILITY=8.2000
11736
PRIMER_RIGHT_3_END_STABILITY=7.9000
11737
PRIMER_PAIR_3_COMPL_ANY=5.00
11738
PRIMER_PAIR_3_COMPL_END=1.00
11739
PRIMER_PRODUCT_SIZE_3=148
11740
PRIMER_PAIR_PENALTY_4=0.4342
11741
PRIMER_LEFT_4_PENALTY=0.156061
11742
PRIMER_RIGHT_4_PENALTY=0.278164
11743
PRIMER_LEFT_4_SEQUENCE=TTCCACAGGAACCTGAGCTT
11744
PRIMER_RIGHT_4_SEQUENCE=ATGGGTCCTCCAAGATCTCC
11745
PRIMER_LEFT_4=121,20
11746
PRIMER_RIGHT_4=266,20
11747
PRIMER_LEFT_4_TM=59.844
11748
PRIMER_RIGHT_4_TM=60.278
11749
PRIMER_LEFT_4_GC_PERCENT=50.000
11750
PRIMER_RIGHT_4_GC_PERCENT=55.000
11751
PRIMER_LEFT_4_SELF_ANY=6.00
11752
PRIMER_RIGHT_4_SELF_ANY=6.00
11753
PRIMER_LEFT_4_SELF_END=5.00
11754
PRIMER_RIGHT_4_SELF_END=2.00
11755
PRIMER_LEFT_4_END_STABILITY=8.2000
11756
PRIMER_RIGHT_4_END_STABILITY=7.9000
11757
PRIMER_PAIR_4_COMPL_ANY=5.00
11758
PRIMER_PAIR_4_COMPL_END=1.00
11759
PRIMER_PRODUCT_SIZE_4=146
11760
=
11761
MARKER_NAME=04.RHAP131FB7.seq
11762
SEQUENCE=TCTAGGACCCCCCTATTTGGGCTCTTCTCNCCGGGTCCTCTGAAAGAACAGCCAGTCTTCCTTTACACTGGAAAGCAGAGAGTGAGTCATTTAGAGGGTCTTATGACCTGCTTCCTGGGAAAGTCAAGATGTTCTTCCTATTTTTAATGATCTACTTTAAATAGGGAAAAATACAAGGACAGAGAAGATTATTTTTGCTTGTGACATTTCCTTCACCAAATTCCTGCAGCTTAAAATACTAGTGTGCCAAAGTATGGTAAAATGTTCTTGAGTTCATCAATACAACTATTTTCTTAAAAGAATTCTTTATCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGGGGGGTGAGCGAGCAGTCTCTCTGTGCATGGAAGAATTGGGGGATTGAACCTAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
11763
INCLUDED_REGION=0,422
11764
TARGET=313,50,TG
11765
PRIMER_COMMENT=CL is CCAAATTCCTGCAGCTTAAAA
11766
PRIMER_COMMENT=CR is AGAGAGACTGCTCGCTCACC
11767
PRIMER_PAIR_PENALTY=1.7380
11768
PRIMER_LEFT_PENALTY=1.628243
11769
PRIMER_RIGHT_PENALTY=0.109760
11770
PRIMER_LEFT_SEQUENCE=CCAAATTCCTGCAGCTTAAAA
11771
PRIMER_RIGHT_SEQUENCE=AGAGAGACTGCTCGCTCACC
11772
PRIMER_LEFT=215,21
11773
PRIMER_RIGHT=388,20
11774
PRIMER_LEFT_TM=59.372
11775
PRIMER_RIGHT_TM=59.890
11776
PRIMER_LEFT_GC_PERCENT=38.095
11777
PRIMER_RIGHT_GC_PERCENT=60.000
11778
PRIMER_LEFT_SELF_ANY=6.00
11779
PRIMER_RIGHT_SELF_ANY=5.00
11780
PRIMER_LEFT_SELF_END=2.00
11781
PRIMER_RIGHT_SELF_END=1.00
11782
PRIMER_LEFT_END_STABILITY=6.6000
11783
PRIMER_RIGHT_END_STABILITY=7.9000
11784
PRIMER_PAIR_COMPL_ANY=4.00
11785
PRIMER_PAIR_COMPL_END=0.00
11786
PRIMER_PRODUCT_SIZE=174
11787
PRIMER_PAIR_PENALTY_1=2.0652
11788
PRIMER_LEFT_1_PENALTY=1.955401
11789
PRIMER_RIGHT_1_PENALTY=0.109760
11790
PRIMER_LEFT_1_SEQUENCE=CCAAATTCCTGCAGCTTAAA
11791
PRIMER_RIGHT_1_SEQUENCE=AGAGAGACTGCTCGCTCACC
11792
PRIMER_LEFT_1=215,20
11793
PRIMER_RIGHT_1=388,20
11794
PRIMER_LEFT_1_TM=58.045
11795
PRIMER_RIGHT_1_TM=59.890
11796
PRIMER_LEFT_1_GC_PERCENT=40.000
11797
PRIMER_RIGHT_1_GC_PERCENT=60.000
11798
PRIMER_LEFT_1_SELF_ANY=6.00
11799
PRIMER_RIGHT_1_SELF_ANY=5.00
11800
PRIMER_LEFT_1_SELF_END=3.00
11801
PRIMER_RIGHT_1_SELF_END=1.00
11802
PRIMER_LEFT_1_END_STABILITY=6.6000
11803
PRIMER_RIGHT_1_END_STABILITY=7.9000
11804
PRIMER_PAIR_1_COMPL_ANY=4.00
11805
PRIMER_PAIR_1_COMPL_END=0.00
11806
PRIMER_PRODUCT_SIZE_1=174
11807
PRIMER_PAIR_PENALTY_2=2.2216
11808
PRIMER_LEFT_2_PENALTY=2.111815
11809
PRIMER_RIGHT_2_PENALTY=0.109760
11810
PRIMER_LEFT_2_SEQUENCE=ACCAAATTCCTGCAGCTTAAA
11811
PRIMER_RIGHT_2_SEQUENCE=AGAGAGACTGCTCGCTCACC
11812
PRIMER_LEFT_2=214,21
11813
PRIMER_RIGHT_2=388,20
11814
PRIMER_LEFT_2_TM=58.888
11815
PRIMER_RIGHT_2_TM=59.890
11816
PRIMER_LEFT_2_GC_PERCENT=38.095
11817
PRIMER_RIGHT_2_GC_PERCENT=60.000
11818
PRIMER_LEFT_2_SELF_ANY=6.00
11819
PRIMER_RIGHT_2_SELF_ANY=5.00
11820
PRIMER_LEFT_2_SELF_END=3.00
11821
PRIMER_RIGHT_2_SELF_END=1.00
11822
PRIMER_LEFT_2_END_STABILITY=6.6000
11823
PRIMER_RIGHT_2_END_STABILITY=7.9000
11824
PRIMER_PAIR_2_COMPL_ANY=4.00
11825
PRIMER_PAIR_2_COMPL_END=0.00
11826
PRIMER_PRODUCT_SIZE_2=175
11827
PRIMER_PAIR_PENALTY_3=2.2377
11828
PRIMER_LEFT_3_PENALTY=2.127927
11829
PRIMER_RIGHT_3_PENALTY=0.109760
11830
PRIMER_LEFT_3_SEQUENCE=ACCAAATTCCTGCAGCTTAAAA
11831
PRIMER_RIGHT_3_SEQUENCE=AGAGAGACTGCTCGCTCACC
11832
PRIMER_LEFT_3=214,22
11833
PRIMER_RIGHT_3=388,20
11834
PRIMER_LEFT_3_TM=60.128
11835
PRIMER_RIGHT_3_TM=59.890
11836
PRIMER_LEFT_3_GC_PERCENT=36.364
11837
PRIMER_RIGHT_3_GC_PERCENT=60.000
11838
PRIMER_LEFT_3_SELF_ANY=6.00
11839
PRIMER_RIGHT_3_SELF_ANY=5.00
11840
PRIMER_LEFT_3_SELF_END=2.00
11841
PRIMER_RIGHT_3_SELF_END=1.00
11842
PRIMER_LEFT_3_END_STABILITY=6.6000
11843
PRIMER_RIGHT_3_END_STABILITY=7.9000
11844
PRIMER_PAIR_3_COMPL_ANY=4.00
11845
PRIMER_PAIR_3_COMPL_END=0.00
11846
PRIMER_PRODUCT_SIZE_3=175
11847
PRIMER_PAIR_PENALTY_4=2.4654
11848
PRIMER_LEFT_4_PENALTY=2.355593
11849
PRIMER_RIGHT_4_PENALTY=0.109760
11850
PRIMER_LEFT_4_SEQUENCE=CCAAATTCCTGCAGCTTAAAAT
11851
PRIMER_RIGHT_4_SEQUENCE=AGAGAGACTGCTCGCTCACC
11852
PRIMER_LEFT_4=215,22
11853
PRIMER_RIGHT_4=388,20
11854
PRIMER_LEFT_4_TM=59.644
11855
PRIMER_RIGHT_4_TM=59.890
11856
PRIMER_LEFT_4_GC_PERCENT=36.364
11857
PRIMER_RIGHT_4_GC_PERCENT=60.000
11858
PRIMER_LEFT_4_SELF_ANY=6.00
11859
PRIMER_RIGHT_4_SELF_ANY=5.00
11860
PRIMER_LEFT_4_SELF_END=2.00
11861
PRIMER_RIGHT_4_SELF_END=1.00
11862
PRIMER_LEFT_4_END_STABILITY=7.2000
11863
PRIMER_RIGHT_4_END_STABILITY=7.9000
11864
PRIMER_PAIR_4_COMPL_ANY=4.00
11865
PRIMER_PAIR_4_COMPL_END=0.00
11866
PRIMER_PRODUCT_SIZE_4=174
11867
=
11868
MARKER_NAME=08.RHAP131FC2.seq
11869
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNCCAGGATATTTTGCCCTTGCAAGGAACATACTACACAGCTCATGCCAAGTTTTTCATGGTTTTTGTGAGAAAATTTTAGGTGCAGCAAAAGAGACGATTGGTTACATTCTCACAGCTGATTAGCTCCAACACAGAGTGGTATAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGATGGGTGGGAAGAGAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
11870
INCLUDED_REGION=22,210
11871
TARGET=167,46,GT
11872
PRIMER_COMMENT=CL is TGCAGCAAAAGAGACGATTG
11873
PRIMER_COMMENT=CR is CCTTCTCTTCCCACCCATC
11874
PRIMER_PAIR_PENALTY=1.6892
11875
PRIMER_LEFT_PENALTY=0.134363
11876
PRIMER_RIGHT_PENALTY=1.554836
11877
PRIMER_LEFT_SEQUENCE=TGCAGCAAAAGAGACGATTG
11878
PRIMER_RIGHT_SEQUENCE=CCTTCTCTTCCCACCCATC
11879
PRIMER_LEFT=102,20
11880
PRIMER_RIGHT=231,19
11881
PRIMER_LEFT_TM=60.134
11882
PRIMER_RIGHT_TM=59.445
11883
PRIMER_LEFT_GC_PERCENT=45.000
11884
PRIMER_RIGHT_GC_PERCENT=57.895
11885
PRIMER_LEFT_SELF_ANY=5.00
11886
PRIMER_RIGHT_SELF_ANY=2.00
11887
PRIMER_LEFT_SELF_END=2.00
11888
PRIMER_RIGHT_SELF_END=0.00
11889
PRIMER_LEFT_END_STABILITY=6.9000
11890
PRIMER_RIGHT_END_STABILITY=8.1000
11891
PRIMER_PAIR_COMPL_ANY=6.00
11892
PRIMER_PAIR_COMPL_END=3.00
11893
PRIMER_PRODUCT_SIZE=130
11894
PRIMER_PAIR_PENALTY_1=1.9606
11895
PRIMER_LEFT_1_PENALTY=0.405717
11896
PRIMER_RIGHT_1_PENALTY=1.554836
11897
PRIMER_LEFT_1_SEQUENCE=GGTGCAGCAAAAGAGACGAT
11898
PRIMER_RIGHT_1_SEQUENCE=CCTTCTCTTCCCACCCATC
11899
PRIMER_LEFT_1=100,20
11900
PRIMER_RIGHT_1=231,19
11901
PRIMER_LEFT_1_TM=60.406
11902
PRIMER_RIGHT_1_TM=59.445
11903
PRIMER_LEFT_1_GC_PERCENT=50.000
11904
PRIMER_RIGHT_1_GC_PERCENT=57.895
11905
PRIMER_LEFT_1_SELF_ANY=5.00
11906
PRIMER_RIGHT_1_SELF_ANY=2.00
11907
PRIMER_LEFT_1_SELF_END=2.00
11908
PRIMER_RIGHT_1_SELF_END=0.00
11909
PRIMER_LEFT_1_END_STABILITY=8.0000
11910
PRIMER_RIGHT_1_END_STABILITY=8.1000
11911
PRIMER_PAIR_1_COMPL_ANY=6.00
11912
PRIMER_PAIR_1_COMPL_END=3.00
11913
PRIMER_PRODUCT_SIZE_1=132
11914
PRIMER_PAIR_PENALTY_2=2.0368
11915
PRIMER_LEFT_2_PENALTY=0.481956
11916
PRIMER_RIGHT_2_PENALTY=1.554836
11917
PRIMER_LEFT_2_SEQUENCE=TCATGCCAAGTTTTTCATGG
11918
PRIMER_RIGHT_2_SEQUENCE=CCTTCTCTTCCCACCCATC
11919
PRIMER_LEFT_2=61,20
11920
PRIMER_RIGHT_2=231,19
11921
PRIMER_LEFT_2_TM=59.518
11922
PRIMER_RIGHT_2_TM=59.445
11923
PRIMER_LEFT_2_GC_PERCENT=40.000
11924
PRIMER_RIGHT_2_GC_PERCENT=57.895
11925
PRIMER_LEFT_2_SELF_ANY=6.00
11926
PRIMER_RIGHT_2_SELF_ANY=2.00
11927
PRIMER_LEFT_2_SELF_END=4.00
11928
PRIMER_RIGHT_2_SELF_END=0.00
11929
PRIMER_LEFT_2_END_STABILITY=8.4000
11930
PRIMER_RIGHT_2_END_STABILITY=8.1000
11931
PRIMER_PAIR_2_COMPL_ANY=4.00
11932
PRIMER_PAIR_2_COMPL_END=3.00
11933
PRIMER_PRODUCT_SIZE_2=171
11934
PRIMER_PAIR_PENALTY_3=2.3350
11935
PRIMER_LEFT_3_PENALTY=0.780133
11936
PRIMER_RIGHT_3_PENALTY=1.554836
11937
PRIMER_LEFT_3_SEQUENCE=TAGGTGCAGCAAAAGAGACG
11938
PRIMER_RIGHT_3_SEQUENCE=CCTTCTCTTCCCACCCATC
11939
PRIMER_LEFT_3=98,20
11940
PRIMER_RIGHT_3=231,19
11941
PRIMER_LEFT_3_TM=59.220
11942
PRIMER_RIGHT_3_TM=59.445
11943
PRIMER_LEFT_3_GC_PERCENT=50.000
11944
PRIMER_RIGHT_3_GC_PERCENT=57.895
11945
PRIMER_LEFT_3_SELF_ANY=5.00
11946
PRIMER_RIGHT_3_SELF_ANY=2.00
11947
PRIMER_LEFT_3_SELF_END=2.00
11948
PRIMER_RIGHT_3_SELF_END=0.00
11949
PRIMER_LEFT_3_END_STABILITY=8.1000
11950
PRIMER_RIGHT_3_END_STABILITY=8.1000
11951
PRIMER_PAIR_3_COMPL_ANY=6.00
11952
PRIMER_PAIR_3_COMPL_END=3.00
11953
PRIMER_PRODUCT_SIZE_3=134
11954
PRIMER_PAIR_PENALTY_4=2.4691
11955
PRIMER_LEFT_4_PENALTY=0.914244
11956
PRIMER_RIGHT_4_PENALTY=1.554836
11957
PRIMER_LEFT_4_SEQUENCE=GCAGCAAAAGAGACGATTGG
11958
PRIMER_RIGHT_4_SEQUENCE=CCTTCTCTTCCCACCCATC
11959
PRIMER_LEFT_4=103,20
11960
PRIMER_RIGHT_4=231,19
11961
PRIMER_LEFT_4_TM=60.914
11962
PRIMER_RIGHT_4_TM=59.445
11963
PRIMER_LEFT_4_GC_PERCENT=50.000
11964
PRIMER_RIGHT_4_GC_PERCENT=57.895
11965
PRIMER_LEFT_4_SELF_ANY=3.00
11966
PRIMER_RIGHT_4_SELF_ANY=2.00
11967
PRIMER_LEFT_4_SELF_END=1.00
11968
PRIMER_RIGHT_4_SELF_END=0.00
11969
PRIMER_LEFT_4_END_STABILITY=8.4000
11970
PRIMER_RIGHT_4_END_STABILITY=8.1000
11971
PRIMER_PAIR_4_COMPL_ANY=6.00
11972
PRIMER_PAIR_4_COMPL_END=4.00
11973
PRIMER_PRODUCT_SIZE_4=129
11974
=
11975
MARKER_NAME=08.RHAP131FC8.seq
11976
SEQUENCE=AAAAANNGNTTAGAGGATCCCCCATAGAAGCTTCTACTATACCAGCTCTAGTAGAAGGGGACAAGCCTTAGTTTATGCAACTGAGTTCATGGCACGGGATTGTAAATGGTACATGAGTCTTATGTTCAAGCATTATTTCTATAAAATCTCACTTTTTAAGTCCAGTGAGTAAGCTGTGAGCTCCGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGTCCCAGCTACTTGGGAGGCTCAGTTCCATGATGTTTATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
11977
INCLUDED_REGION=0,293
11978
TARGET=187,64,GT
11979
PRIMER_COMMENT=CL is TCCAGTGAGTAAGCTGTGAGCT
11980
PRIMER_COMMENT=CR is CATCATGGAACTGAGCCTCC
11981
PRIMER_PAIR_PENALTY=3.3172
11982
PRIMER_LEFT_PENALTY=2.694680
11983
PRIMER_RIGHT_PENALTY=0.622472
11984
PRIMER_LEFT_SEQUENCE=TCCAGTGAGTAAGCTGTGAGCT
11985
PRIMER_RIGHT_SEQUENCE=CATCATGGAACTGAGCCTCC
11986
PRIMER_LEFT=160,22
11987
PRIMER_RIGHT=285,20
11988
PRIMER_LEFT_TM=59.305
11989
PRIMER_RIGHT_TM=60.622
11990
PRIMER_LEFT_GC_PERCENT=50.000
11991
PRIMER_RIGHT_GC_PERCENT=55.000
11992
PRIMER_LEFT_SELF_ANY=5.00
11993
PRIMER_RIGHT_SELF_ANY=5.00
11994
PRIMER_LEFT_SELF_END=5.00
11995
PRIMER_RIGHT_SELF_END=3.00
11996
PRIMER_LEFT_END_STABILITY=7.9000
11997
PRIMER_RIGHT_END_STABILITY=9.4000
11998
PRIMER_PAIR_COMPL_ANY=5.00
11999
PRIMER_PAIR_COMPL_END=2.00
12000
PRIMER_PRODUCT_SIZE=126
12001
PRIMER_PAIR_PENALTY_1=3.3172
12002
PRIMER_LEFT_1_PENALTY=2.694680
12003
PRIMER_RIGHT_1_PENALTY=0.622472
12004
PRIMER_LEFT_1_SEQUENCE=CCAGTGAGTAAGCTGTGAGCTC
12005
PRIMER_RIGHT_1_SEQUENCE=CATCATGGAACTGAGCCTCC
12006
PRIMER_LEFT_1=161,22
12007
PRIMER_RIGHT_1=285,20
12008
PRIMER_LEFT_1_TM=59.305
12009
PRIMER_RIGHT_1_TM=60.622
12010
PRIMER_LEFT_1_GC_PERCENT=54.545
12011
PRIMER_RIGHT_1_GC_PERCENT=55.000
12012
PRIMER_LEFT_1_SELF_ANY=6.00
12013
PRIMER_RIGHT_1_SELF_ANY=5.00
12014
PRIMER_LEFT_1_SELF_END=6.00
12015
PRIMER_RIGHT_1_SELF_END=3.00
12016
PRIMER_LEFT_1_END_STABILITY=7.9000
12017
PRIMER_RIGHT_1_END_STABILITY=9.4000
12018
PRIMER_PAIR_1_COMPL_ANY=4.00
12019
PRIMER_PAIR_1_COMPL_END=3.00
12020
PRIMER_PRODUCT_SIZE_1=125
12021
PRIMER_PAIR_PENALTY_2=3.3649
12022
PRIMER_LEFT_2_PENALTY=2.742415
12023
PRIMER_RIGHT_2_PENALTY=0.622472
12024
PRIMER_LEFT_2_SEQUENCE=TCCAGTGAGTAAGCTGTGAGC
12025
PRIMER_RIGHT_2_SEQUENCE=CATCATGGAACTGAGCCTCC
12026
PRIMER_LEFT_2=160,21
12027
PRIMER_RIGHT_2=285,20
12028
PRIMER_LEFT_2_TM=58.258
12029
PRIMER_RIGHT_2_TM=60.622
12030
PRIMER_LEFT_2_GC_PERCENT=52.381
12031
PRIMER_RIGHT_2_GC_PERCENT=55.000
12032
PRIMER_LEFT_2_SELF_ANY=4.00
12033
PRIMER_RIGHT_2_SELF_ANY=5.00
12034
PRIMER_LEFT_2_SELF_END=3.00
12035
PRIMER_RIGHT_2_SELF_END=3.00
12036
PRIMER_LEFT_2_END_STABILITY=8.2000
12037
PRIMER_RIGHT_2_END_STABILITY=9.4000
12038
PRIMER_PAIR_2_COMPL_ANY=5.00
12039
PRIMER_PAIR_2_COMPL_END=1.00
12040
PRIMER_PRODUCT_SIZE_2=126
12041
PRIMER_PAIR_PENALTY_3=3.4576
12042
PRIMER_LEFT_3_PENALTY=2.835083
12043
PRIMER_RIGHT_3_PENALTY=0.622472
12044
PRIMER_LEFT_3_SEQUENCE=GTCCAGTGAGTAAGCTGTGAGC
12045
PRIMER_RIGHT_3_SEQUENCE=CATCATGGAACTGAGCCTCC
12046
PRIMER_LEFT_3=159,22
12047
PRIMER_RIGHT_3=285,20
12048
PRIMER_LEFT_3_TM=59.165
12049
PRIMER_RIGHT_3_TM=60.622
12050
PRIMER_LEFT_3_GC_PERCENT=54.545
12051
PRIMER_RIGHT_3_GC_PERCENT=55.000
12052
PRIMER_LEFT_3_SELF_ANY=4.00
12053
PRIMER_RIGHT_3_SELF_ANY=5.00
12054
PRIMER_LEFT_3_SELF_END=3.00
12055
PRIMER_RIGHT_3_SELF_END=3.00
12056
PRIMER_LEFT_3_END_STABILITY=8.2000
12057
PRIMER_RIGHT_3_END_STABILITY=9.4000
12058
PRIMER_PAIR_3_COMPL_ANY=5.00
12059
PRIMER_PAIR_3_COMPL_END=1.00
12060
PRIMER_PRODUCT_SIZE_3=127
12061
PRIMER_PAIR_PENALTY_4=3.5485
12062
PRIMER_LEFT_4_PENALTY=1.208168
12063
PRIMER_RIGHT_4_PENALTY=2.340342
12064
PRIMER_LEFT_4_SEQUENCE=GTGAGTAAGCTGTGAGCTCCG
12065
PRIMER_RIGHT_4_SEQUENCE=AAACATCATGGAACTGAGCCT
12066
PRIMER_LEFT_4=164,21
12067
PRIMER_RIGHT_4=288,21
12068
PRIMER_LEFT_4_TM=60.208
12069
PRIMER_RIGHT_4_TM=58.660
12070
PRIMER_LEFT_4_GC_PERCENT=57.143
12071
PRIMER_RIGHT_4_GC_PERCENT=42.857
12072
PRIMER_LEFT_4_SELF_ANY=6.00
12073
PRIMER_RIGHT_4_SELF_ANY=5.00
12074
PRIMER_LEFT_4_SELF_END=2.00
12075
PRIMER_RIGHT_4_SELF_END=3.00
12076
PRIMER_LEFT_4_END_STABILITY=9.9000
12077
PRIMER_RIGHT_4_END_STABILITY=9.4000
12078
PRIMER_PAIR_4_COMPL_ANY=4.00
12079
PRIMER_PAIR_4_COMPL_END=3.00
12080
PRIMER_PRODUCT_SIZE_4=125
12081
=
12082
MARKER_NAME=10.RHAP131FD1.seq
12083
SEQUENCE=NNNNNNNNNNNGAACTATATCCCCAGCTCTTAGGAATGGAAATTCAGGATTCCAAAAGGAAGCAAAAAATAAAGCCTCACCCATAACCCCACATTCTTTGTGCACGCCCCTCTCCTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGGTGAGAATTGAACCCGGAGTCTGTCTAAGCTAAGTGNNNNNNNNNNNNNNGAGCCACCGCTAGCCACAGCGTTGTTTAATTACCTGTTGCTCTAGTTCAGAACTTTTACTGTGGTTAACCATGTTGAGCTACAGTGTTTCACTGTAACCACTTCTCATGGCTCGCTCCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
12084
INCLUDED_REGION=11,326
12085
TARGET=118,46,TG
12086
TARGET=203,14,CT
12087
PRIMER_COMMENT=CL is CAGGATTCCAAAAGGAAGCA
12088
PRIMER_COMMENT=CR is GACTCCGGGTTCAATTCTCA
12089
PRIMER_PAIR_PENALTY=0.2338
12090
PRIMER_LEFT_PENALTY=0.183944
12091
PRIMER_RIGHT_PENALTY=0.049859
12092
PRIMER_LEFT_SEQUENCE=CAGGATTCCAAAAGGAAGCA
12093
PRIMER_RIGHT_SEQUENCE=GACTCCGGGTTCAATTCTCA
12094
PRIMER_LEFT=44,20
12095
PRIMER_RIGHT=187,20
12096
PRIMER_LEFT_TM=60.184
12097
PRIMER_RIGHT_TM=60.050
12098
PRIMER_LEFT_GC_PERCENT=45.000
12099
PRIMER_RIGHT_GC_PERCENT=50.000
12100
PRIMER_LEFT_SELF_ANY=5.00
12101
PRIMER_RIGHT_SELF_ANY=4.00
12102
PRIMER_LEFT_SELF_END=2.00
12103
PRIMER_RIGHT_SELF_END=1.00
12104
PRIMER_LEFT_END_STABILITY=8.5000
12105
PRIMER_RIGHT_END_STABILITY=6.7000
12106
PRIMER_PAIR_COMPL_ANY=4.00
12107
PRIMER_PAIR_COMPL_END=0.00
12108
PRIMER_PRODUCT_SIZE=144
12109
PRIMER_PAIR_PENALTY_1=0.7855
12110
PRIMER_LEFT_1_PENALTY=0.735594
12111
PRIMER_RIGHT_1_PENALTY=0.049859
12112
PRIMER_LEFT_1_SEQUENCE=CCCAGCTCTTAGGAATGGAA
12113
PRIMER_RIGHT_1_SEQUENCE=GACTCCGGGTTCAATTCTCA
12114
PRIMER_LEFT_1=21,20
12115
PRIMER_RIGHT_1=187,20
12116
PRIMER_LEFT_1_TM=59.264
12117
PRIMER_RIGHT_1_TM=60.050
12118
PRIMER_LEFT_1_GC_PERCENT=50.000
12119
PRIMER_RIGHT_1_GC_PERCENT=50.000
12120
PRIMER_LEFT_1_SELF_ANY=4.00
12121
PRIMER_RIGHT_1_SELF_ANY=4.00
12122
PRIMER_LEFT_1_SELF_END=0.00
12123
PRIMER_RIGHT_1_SELF_END=1.00
12124
PRIMER_LEFT_1_END_STABILITY=8.5000
12125
PRIMER_RIGHT_1_END_STABILITY=6.7000
12126
PRIMER_PAIR_1_COMPL_ANY=6.00
12127
PRIMER_PAIR_1_COMPL_END=5.00
12128
PRIMER_PRODUCT_SIZE_1=167
12129
PRIMER_PAIR_PENALTY_2=0.8054
12130
PRIMER_LEFT_2_PENALTY=0.755541
12131
PRIMER_RIGHT_2_PENALTY=0.049859
12132
PRIMER_LEFT_2_SEQUENCE=TCAGGATTCCAAAAGGAAGC
12133
PRIMER_RIGHT_2_SEQUENCE=GACTCCGGGTTCAATTCTCA
12134
PRIMER_LEFT_2=43,20
12135
PRIMER_RIGHT_2=187,20
12136
PRIMER_LEFT_2_TM=59.244
12137
PRIMER_RIGHT_2_TM=60.050
12138
PRIMER_LEFT_2_GC_PERCENT=45.000
12139
PRIMER_RIGHT_2_GC_PERCENT=50.000
12140
PRIMER_LEFT_2_SELF_ANY=5.00
12141
PRIMER_RIGHT_2_SELF_ANY=4.00
12142
PRIMER_LEFT_2_SELF_END=4.00
12143
PRIMER_RIGHT_2_SELF_END=1.00
12144
PRIMER_LEFT_2_END_STABILITY=8.2000
12145
PRIMER_RIGHT_2_END_STABILITY=6.7000
12146
PRIMER_PAIR_2_COMPL_ANY=4.00
12147
PRIMER_PAIR_2_COMPL_END=2.00
12148
PRIMER_PRODUCT_SIZE_2=145
12149
PRIMER_PAIR_PENALTY_3=0.9150
12150
PRIMER_LEFT_3_PENALTY=0.865157
12151
PRIMER_RIGHT_3_PENALTY=0.049859
12152
PRIMER_LEFT_3_SEQUENCE=GGATTCCAAAAGGAAGCAAA
12153
PRIMER_RIGHT_3_SEQUENCE=GACTCCGGGTTCAATTCTCA
12154
PRIMER_LEFT_3=46,20
12155
PRIMER_RIGHT_3=187,20
12156
PRIMER_LEFT_3_TM=59.135
12157
PRIMER_RIGHT_3_TM=60.050
12158
PRIMER_LEFT_3_GC_PERCENT=40.000
12159
PRIMER_RIGHT_3_GC_PERCENT=50.000
12160
PRIMER_LEFT_3_SELF_ANY=5.00
12161
PRIMER_RIGHT_3_SELF_ANY=4.00
12162
PRIMER_LEFT_3_SELF_END=0.00
12163
PRIMER_RIGHT_3_SELF_END=1.00
12164
PRIMER_LEFT_3_END_STABILITY=8.8000
12165
PRIMER_RIGHT_3_END_STABILITY=6.7000
12166
PRIMER_PAIR_3_COMPL_ANY=4.00
12167
PRIMER_PAIR_3_COMPL_END=0.00
12168
PRIMER_PRODUCT_SIZE_3=142
12169
PRIMER_PAIR_PENALTY_4=1.0469
12170
PRIMER_LEFT_4_PENALTY=0.183944
12171
PRIMER_RIGHT_4_PENALTY=0.862979
12172
PRIMER_LEFT_4_SEQUENCE=CAGGATTCCAAAAGGAAGCA
12173
PRIMER_RIGHT_4_SEQUENCE=CAGACTCCGGGTTCAATTCT
12174
PRIMER_LEFT_4=44,20
12175
PRIMER_RIGHT_4=189,20
12176
PRIMER_LEFT_4_TM=60.184
12177
PRIMER_RIGHT_4_TM=59.137
12178
PRIMER_LEFT_4_GC_PERCENT=45.000
12179
PRIMER_RIGHT_4_GC_PERCENT=50.000
12180
PRIMER_LEFT_4_SELF_ANY=5.00
12181
PRIMER_RIGHT_4_SELF_ANY=4.00
12182
PRIMER_LEFT_4_SELF_END=2.00
12183
PRIMER_RIGHT_4_SELF_END=0.00
12184
PRIMER_LEFT_4_END_STABILITY=8.5000
12185
PRIMER_RIGHT_4_END_STABILITY=6.6000
12186
PRIMER_PAIR_4_COMPL_ANY=4.00
12187
PRIMER_PAIR_4_COMPL_END=2.00
12188
PRIMER_PRODUCT_SIZE_4=146
12189
=
12190
MARKER_NAME=10.RHAP131FD7.seq
12191
SEQUENCE=NNNNNNNNNNNNCACTACTGCAACAGTGTTTCCACTTCAGCATGTGTGGGTGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATATGGGTATCTCTATCTGTATCTGTGTATCTCCTTTATTTATTGATGACTTTAGATGCAGATTTTTTAGGGTGGAGGATGTCCTTGTTTTGATCACATGTTATTTGAAGAACACAATTCCCAGCTAAAAGAATTTAGCTATTTTAGTTGTATCTGCAATGAAAATGCAACTTTTCAGTGAAATTAGCCACGGGTTGAGTTTATGATGAGATGCTCTTTGGAAGACAGAAATGTACTCTCATTTATAAGTTCAAGTCTCATTGAGAATACTCCAAGATTTATTTGTTTACTTTTCAAGAAAAAAAATTGATTTACAGTCTCGAACACAAGAACAAAGCCACAGACAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
12192
INCLUDED_REGION=12,433
12193
TARGET=53,42,GT
12194
PRIMER_COMMENT=CL is TTCCACTTCAGCATGTGTGG
12195
PRIMER_COMMENT=CR is CAAGGACATCCTCCACCCTA
12196
PRIMER_PAIR_PENALTY=0.8027
12197
PRIMER_LEFT_PENALTY=0.722400
12198
PRIMER_RIGHT_PENALTY=0.080313
12199
PRIMER_LEFT_SEQUENCE=TTCCACTTCAGCATGTGTGG
12200
PRIMER_RIGHT_SEQUENCE=CAAGGACATCCTCCACCCTA
12201
PRIMER_LEFT=29,20
12202
PRIMER_RIGHT=182,20
12203
PRIMER_LEFT_TM=60.722
12204
PRIMER_RIGHT_TM=59.920
12205
PRIMER_LEFT_GC_PERCENT=50.000
12206
PRIMER_RIGHT_GC_PERCENT=55.000
12207
PRIMER_LEFT_SELF_ANY=4.00
12208
PRIMER_RIGHT_SELF_ANY=6.00
12209
PRIMER_LEFT_SELF_END=4.00
12210
PRIMER_RIGHT_SELF_END=2.00
12211
PRIMER_LEFT_END_STABILITY=8.2000
12212
PRIMER_RIGHT_END_STABILITY=8.7000
12213
PRIMER_PAIR_COMPL_ANY=5.00
12214
PRIMER_PAIR_COMPL_END=3.00
12215
PRIMER_PRODUCT_SIZE=154
12216
PRIMER_PAIR_PENALTY_1=1.3033
12217
PRIMER_LEFT_1_PENALTY=1.223037
12218
PRIMER_RIGHT_1_PENALTY=0.080313
12219
PRIMER_LEFT_1_SEQUENCE=CACTTCAGCATGTGTGGGTG
12220
PRIMER_RIGHT_1_SEQUENCE=CAAGGACATCCTCCACCCTA
12221
PRIMER_LEFT_1=32,20
12222
PRIMER_RIGHT_1=182,20
12223
PRIMER_LEFT_1_TM=61.223
12224
PRIMER_RIGHT_1_TM=59.920
12225
PRIMER_LEFT_1_GC_PERCENT=55.000
12226
PRIMER_RIGHT_1_GC_PERCENT=55.000
12227
PRIMER_LEFT_1_SELF_ANY=4.00
12228
PRIMER_RIGHT_1_SELF_ANY=6.00
12229
PRIMER_LEFT_1_SELF_END=2.00
12230
PRIMER_RIGHT_1_SELF_END=2.00
12231
PRIMER_LEFT_1_END_STABILITY=9.4000
12232
PRIMER_RIGHT_1_END_STABILITY=8.7000
12233
PRIMER_PAIR_1_COMPL_ANY=5.00
12234
PRIMER_PAIR_1_COMPL_END=4.00
12235
PRIMER_PRODUCT_SIZE_1=151
12236
PRIMER_PAIR_PENALTY_2=1.4115
12237
PRIMER_LEFT_2_PENALTY=1.331234
12238
PRIMER_RIGHT_2_PENALTY=0.080313
12239
PRIMER_LEFT_2_SEQUENCE=TGCAACAGTGTTTCCACTTCA
12240
PRIMER_RIGHT_2_SEQUENCE=CAAGGACATCCTCCACCCTA
12241
PRIMER_LEFT_2=18,21
12242
PRIMER_RIGHT_2=182,20
12243
PRIMER_LEFT_2_TM=60.331
12244
PRIMER_RIGHT_2_TM=59.920
12245
PRIMER_LEFT_2_GC_PERCENT=42.857
12246
PRIMER_RIGHT_2_GC_PERCENT=55.000
12247
PRIMER_LEFT_2_SELF_ANY=7.00
12248
PRIMER_RIGHT_2_SELF_ANY=6.00
12249
PRIMER_LEFT_2_SELF_END=1.00
12250
PRIMER_RIGHT_2_SELF_END=2.00
12251
PRIMER_LEFT_2_END_STABILITY=7.0000
12252
PRIMER_RIGHT_2_END_STABILITY=8.7000
12253
PRIMER_PAIR_2_COMPL_ANY=3.00
12254
PRIMER_PAIR_2_COMPL_END=0.00
12255
PRIMER_PRODUCT_SIZE_2=165
12256
PRIMER_PAIR_PENALTY_3=1.4920
12257
PRIMER_LEFT_3_PENALTY=0.722400
12258
PRIMER_RIGHT_3_PENALTY=0.769636
12259
PRIMER_LEFT_3_SEQUENCE=TTCCACTTCAGCATGTGTGG
12260
PRIMER_RIGHT_3_SEQUENCE=ACAAGGACATCCTCCACCCT
12261
PRIMER_LEFT_3=29,20
12262
PRIMER_RIGHT_3=183,20
12263
PRIMER_LEFT_3_TM=60.722
12264
PRIMER_RIGHT_3_TM=60.770
12265
PRIMER_LEFT_3_GC_PERCENT=50.000
12266
PRIMER_RIGHT_3_GC_PERCENT=55.000
12267
PRIMER_LEFT_3_SELF_ANY=4.00
12268
PRIMER_RIGHT_3_SELF_ANY=6.00
12269
PRIMER_LEFT_3_SELF_END=4.00
12270
PRIMER_RIGHT_3_SELF_END=0.00
12271
PRIMER_LEFT_3_END_STABILITY=8.2000
12272
PRIMER_RIGHT_3_END_STABILITY=9.1000
12273
PRIMER_PAIR_3_COMPL_ANY=5.00
12274
PRIMER_PAIR_3_COMPL_END=3.00
12275
PRIMER_PRODUCT_SIZE_3=155
12276
PRIMER_PAIR_PENALTY_4=1.7308
12277
PRIMER_LEFT_4_PENALTY=1.650466
12278
PRIMER_RIGHT_4_PENALTY=0.080313
12279
PRIMER_LEFT_4_SEQUENCE=CAGTGTTTCCACTTCAGCATG
12280
PRIMER_RIGHT_4_SEQUENCE=CAAGGACATCCTCCACCCTA
12281
PRIMER_LEFT_4=23,21
12282
PRIMER_RIGHT_4=182,20
12283
PRIMER_LEFT_4_TM=59.350
12284
PRIMER_RIGHT_4_TM=59.920
12285
PRIMER_LEFT_4_GC_PERCENT=47.619
12286
PRIMER_RIGHT_4_GC_PERCENT=55.000
12287
PRIMER_LEFT_4_SELF_ANY=4.00
12288
PRIMER_RIGHT_4_SELF_ANY=6.00
12289
PRIMER_LEFT_4_SELF_END=4.00
12290
PRIMER_RIGHT_4_SELF_END=2.00
12291
PRIMER_LEFT_4_END_STABILITY=8.4000
12292
PRIMER_RIGHT_4_END_STABILITY=8.7000
12293
PRIMER_PAIR_4_COMPL_ANY=4.00
12294
PRIMER_PAIR_4_COMPL_END=1.00
12295
PRIMER_PRODUCT_SIZE_4=160
12296
=
12297
MARKER_NAME=11.RHAP131FD2.seq
12298
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNCCAACCATCAACCGAAGCACAAGAACAGACGTGCGTCTTTTGGCTCGCAGTGATTTTTAATGGTAGTCATCCGGACATACGACTTAGATACAATGAGACTACAAACTAACAGACAAGATTCGGGCAAAGCAGAAAAACAATGAAGCCAGCATCCTGATCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGCATGCCTGCCTGTCAATTGTGAGGTAAGACAGGAAGACTTGGTCTCCTGCCAGATCTCCATATCTTCCCGCTCTCCTTTAACTCCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
12299
INCLUDED_REGION=22,297
12300
TARGET=183,45,TG
12301
PRIMER_COMMENT=CL is AAACAATGAAGCCAGCATCC
12302
PRIMER_COMMENT=CR is TCTGGCAGGAGACCAAGTCT
12303
PRIMER_PAIR_PENALTY=0.0954
12304
PRIMER_LEFT_PENALTY=0.080962
12305
PRIMER_RIGHT_PENALTY=0.014419
12306
PRIMER_LEFT_SEQUENCE=AAACAATGAAGCCAGCATCC
12307
PRIMER_RIGHT_SEQUENCE=TCTGGCAGGAGACCAAGTCT
12308
PRIMER_LEFT=156,20
12309
PRIMER_RIGHT=285,20
12310
PRIMER_LEFT_TM=60.081
12311
PRIMER_RIGHT_TM=59.986
12312
PRIMER_LEFT_GC_PERCENT=45.000
12313
PRIMER_RIGHT_GC_PERCENT=55.000
12314
PRIMER_LEFT_SELF_ANY=4.00
12315
PRIMER_RIGHT_SELF_ANY=5.00
12316
PRIMER_LEFT_SELF_END=2.00
12317
PRIMER_RIGHT_SELF_END=5.00
12318
PRIMER_LEFT_END_STABILITY=8.1000
12319
PRIMER_RIGHT_END_STABILITY=6.1000
12320
PRIMER_PAIR_COMPL_ANY=5.00
12321
PRIMER_PAIR_COMPL_END=0.00
12322
PRIMER_PRODUCT_SIZE=130
12323
PRIMER_PAIR_PENALTY_1=0.2032
12324
PRIMER_LEFT_1_PENALTY=0.188746
12325
PRIMER_RIGHT_1_PENALTY=0.014419
12326
PRIMER_LEFT_1_SEQUENCE=ATGAAGCCAGCATCCTGATC
12327
PRIMER_RIGHT_1_SEQUENCE=TCTGGCAGGAGACCAAGTCT
12328
PRIMER_LEFT_1=161,20
12329
PRIMER_RIGHT_1=285,20
12330
PRIMER_LEFT_1_TM=60.189
12331
PRIMER_RIGHT_1_TM=59.986
12332
PRIMER_LEFT_1_GC_PERCENT=50.000
12333
PRIMER_RIGHT_1_GC_PERCENT=55.000
12334
PRIMER_LEFT_1_SELF_ANY=6.00
12335
PRIMER_RIGHT_1_SELF_ANY=5.00
12336
PRIMER_LEFT_1_SELF_END=4.00
12337
PRIMER_RIGHT_1_SELF_END=5.00
12338
PRIMER_LEFT_1_END_STABILITY=6.6000
12339
PRIMER_RIGHT_1_END_STABILITY=6.1000
12340
PRIMER_PAIR_1_COMPL_ANY=5.00
12341
PRIMER_PAIR_1_COMPL_END=2.00
12342
PRIMER_PRODUCT_SIZE_1=125
12343
PRIMER_PAIR_PENALTY_2=0.3147
12344
PRIMER_LEFT_2_PENALTY=0.300243
12345
PRIMER_RIGHT_2_PENALTY=0.014419
12346
PRIMER_LEFT_2_SEQUENCE=AACAATGAAGCCAGCATCCT
12347
PRIMER_RIGHT_2_SEQUENCE=TCTGGCAGGAGACCAAGTCT
12348
PRIMER_LEFT_2=157,20
12349
PRIMER_RIGHT_2=285,20
12350
PRIMER_LEFT_2_TM=59.700
12351
PRIMER_RIGHT_2_TM=59.986
12352
PRIMER_LEFT_2_GC_PERCENT=45.000
12353
PRIMER_RIGHT_2_GC_PERCENT=55.000
12354
PRIMER_LEFT_2_SELF_ANY=4.00
12355
PRIMER_RIGHT_2_SELF_ANY=5.00
12356
PRIMER_LEFT_2_SELF_END=4.00
12357
PRIMER_RIGHT_2_SELF_END=5.00
12358
PRIMER_LEFT_2_END_STABILITY=7.8000
12359
PRIMER_RIGHT_2_END_STABILITY=6.1000
12360
PRIMER_PAIR_2_COMPL_ANY=5.00
12361
PRIMER_PAIR_2_COMPL_END=1.00
12362
PRIMER_PRODUCT_SIZE_2=129
12363
PRIMER_PAIR_PENALTY_3=0.3559
12364
PRIMER_LEFT_3_PENALTY=0.341522
12365
PRIMER_RIGHT_3_PENALTY=0.014419
12366
PRIMER_LEFT_3_SEQUENCE=AATGAAGCCAGCATCCTGAT
12367
PRIMER_RIGHT_3_SEQUENCE=TCTGGCAGGAGACCAAGTCT
12368
PRIMER_LEFT_3=160,20
12369
PRIMER_RIGHT_3=285,20
12370
PRIMER_LEFT_3_TM=59.658
12371
PRIMER_RIGHT_3_TM=59.986
12372
PRIMER_LEFT_3_GC_PERCENT=45.000
12373
PRIMER_RIGHT_3_GC_PERCENT=55.000
12374
PRIMER_LEFT_3_SELF_ANY=6.00
12375
PRIMER_RIGHT_3_SELF_ANY=5.00
12376
PRIMER_LEFT_3_SELF_END=4.00
12377
PRIMER_RIGHT_3_SELF_END=5.00
12378
PRIMER_LEFT_3_END_STABILITY=6.6000
12379
PRIMER_RIGHT_3_END_STABILITY=6.1000
12380
PRIMER_PAIR_3_COMPL_ANY=5.00
12381
PRIMER_PAIR_3_COMPL_END=1.00
12382
PRIMER_PRODUCT_SIZE_3=126
12383
PRIMER_PAIR_PENALTY_4=0.4300
12384
PRIMER_LEFT_4_PENALTY=0.080962
12385
PRIMER_RIGHT_4_PENALTY=0.349041
12386
PRIMER_LEFT_4_SEQUENCE=AAACAATGAAGCCAGCATCC
12387
PRIMER_RIGHT_4_SEQUENCE=GAGATCTGGCAGGAGACCAA
12388
PRIMER_LEFT_4=156,20
12389
PRIMER_RIGHT_4=289,20
12390
PRIMER_LEFT_4_TM=60.081
12391
PRIMER_RIGHT_4_TM=60.349
12392
PRIMER_LEFT_4_GC_PERCENT=45.000
12393
PRIMER_RIGHT_4_GC_PERCENT=55.000
12394
PRIMER_LEFT_4_SELF_ANY=4.00
12395
PRIMER_RIGHT_4_SELF_ANY=6.00
12396
PRIMER_LEFT_4_SELF_END=2.00
12397
PRIMER_RIGHT_4_SELF_END=0.00
12398
PRIMER_LEFT_4_END_STABILITY=8.1000
12399
PRIMER_RIGHT_4_END_STABILITY=8.2000
12400
PRIMER_PAIR_4_COMPL_ANY=6.00
12401
PRIMER_PAIR_4_COMPL_END=0.00
12402
PRIMER_PRODUCT_SIZE_4=134
12403
=
12404
MARKER_NAME=13.RHAP131FE1.seq
12405
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCATGCCTGGAAGCCACATTGACTTTATATTGAAATAATTCTTTTTTAAAAAGTTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTGCGCATGTGTGCTCATGCCCTTGTGTGCATGATAAGTGTATGTAGGCTTTTGTGCCACAGCAAATGTGTAGAAGTCAAAGGAAAACATTCTGAAACGAGTTCTATCTACTTTTCTGTGGATTCCAGGGACTGAACCTGTGCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
12406
INCLUDED_REGION=21,241
12407
TARGET=77,38,TG
12408
PRIMER_COMMENT=CL is CCTGGAAGCCACATTGACTT
12409
PRIMER_COMMENT=CR is TATCATGCACACAAGGGCAT
12410
PRIMER_PAIR_PENALTY=0.1558
12411
PRIMER_LEFT_PENALTY=0.111077
12412
PRIMER_RIGHT_PENALTY=0.044752
12413
PRIMER_LEFT_SEQUENCE=CCTGGAAGCCACATTGACTT
12414
PRIMER_RIGHT_SEQUENCE=TATCATGCACACAAGGGCAT
12415
PRIMER_LEFT=25,20
12416
PRIMER_RIGHT=151,20
12417
PRIMER_LEFT_TM=60.111
12418
PRIMER_RIGHT_TM=59.955
12419
PRIMER_LEFT_GC_PERCENT=50.000
12420
PRIMER_RIGHT_GC_PERCENT=45.000
12421
PRIMER_LEFT_SELF_ANY=6.00
12422
PRIMER_RIGHT_SELF_ANY=4.00
12423
PRIMER_LEFT_SELF_END=6.00
12424
PRIMER_RIGHT_SELF_END=4.00
12425
PRIMER_LEFT_END_STABILITY=6.4000
12426
PRIMER_RIGHT_END_STABILITY=9.6000
12427
PRIMER_PAIR_COMPL_ANY=3.00
12428
PRIMER_PAIR_COMPL_END=2.00
12429
PRIMER_PRODUCT_SIZE=127
12430
PRIMER_PAIR_PENALTY_1=0.2066
12431
PRIMER_LEFT_1_PENALTY=0.111077
12432
PRIMER_RIGHT_1_PENALTY=0.095506
12433
PRIMER_LEFT_1_SEQUENCE=CCTGGAAGCCACATTGACTT
12434
PRIMER_RIGHT_1_SEQUENCE=CTGTGGCACAAAAGCCTACA
12435
PRIMER_LEFT_1=25,20
12436
PRIMER_RIGHT_1=177,20
12437
PRIMER_LEFT_1_TM=60.111
12438
PRIMER_RIGHT_1_TM=59.904
12439
PRIMER_LEFT_1_GC_PERCENT=50.000
12440
PRIMER_RIGHT_1_GC_PERCENT=50.000
12441
PRIMER_LEFT_1_SELF_ANY=6.00
12442
PRIMER_RIGHT_1_SELF_ANY=7.00
12443
PRIMER_LEFT_1_SELF_END=6.00
12444
PRIMER_RIGHT_1_SELF_END=2.00
12445
PRIMER_LEFT_1_END_STABILITY=6.4000
12446
PRIMER_RIGHT_1_END_STABILITY=5.7000
12447
PRIMER_PAIR_1_COMPL_ANY=6.00
12448
PRIMER_PAIR_1_COMPL_END=1.00
12449
PRIMER_PRODUCT_SIZE_1=153
12450
PRIMER_PAIR_PENALTY_2=0.2683
12451
PRIMER_LEFT_2_PENALTY=0.111077
12452
PRIMER_RIGHT_2_PENALTY=0.157240
12453
PRIMER_LEFT_2_SEQUENCE=CCTGGAAGCCACATTGACTT
12454
PRIMER_RIGHT_2_SEQUENCE=ACATTTGCTGTGGCACAAAA
12455
PRIMER_LEFT_2=25,20
12456
PRIMER_RIGHT_2=184,20
12457
PRIMER_LEFT_2_TM=60.111
12458
PRIMER_RIGHT_2_TM=60.157
12459
PRIMER_LEFT_2_GC_PERCENT=50.000
12460
PRIMER_RIGHT_2_GC_PERCENT=40.000
12461
PRIMER_LEFT_2_SELF_ANY=6.00
12462
PRIMER_RIGHT_2_SELF_ANY=7.00
12463
PRIMER_LEFT_2_SELF_END=6.00
12464
PRIMER_RIGHT_2_SELF_END=5.00
12465
PRIMER_LEFT_2_END_STABILITY=6.4000
12466
PRIMER_RIGHT_2_END_STABILITY=7.6000
12467
PRIMER_PAIR_2_COMPL_ANY=6.00
12468
PRIMER_PAIR_2_COMPL_END=2.00
12469
PRIMER_PRODUCT_SIZE_2=160
12470
PRIMER_PAIR_PENALTY_3=0.4046
12471
PRIMER_LEFT_3_PENALTY=0.111077
12472
PRIMER_RIGHT_3_PENALTY=0.293496
12473
PRIMER_LEFT_3_SEQUENCE=CCTGGAAGCCACATTGACTT
12474
PRIMER_RIGHT_3_SEQUENCE=CATTTGCTGTGGCACAAAAG
12475
PRIMER_LEFT_3=25,20
12476
PRIMER_RIGHT_3=183,20
12477
PRIMER_LEFT_3_TM=60.111
12478
PRIMER_RIGHT_3_TM=60.293
12479
PRIMER_LEFT_3_GC_PERCENT=50.000
12480
PRIMER_RIGHT_3_GC_PERCENT=45.000
12481
PRIMER_LEFT_3_SELF_ANY=6.00
12482
PRIMER_RIGHT_3_SELF_ANY=7.00
12483
PRIMER_LEFT_3_SELF_END=6.00
12484
PRIMER_RIGHT_3_SELF_END=5.00
12485
PRIMER_LEFT_3_END_STABILITY=6.4000
12486
PRIMER_RIGHT_3_END_STABILITY=7.3000
12487
PRIMER_PAIR_3_COMPL_ANY=6.00
12488
PRIMER_PAIR_3_COMPL_END=3.00
12489
PRIMER_PRODUCT_SIZE_3=159
12490
PRIMER_PAIR_PENALTY_4=0.9122
12491
PRIMER_LEFT_4_PENALTY=0.111077
12492
PRIMER_RIGHT_4_PENALTY=0.801088
12493
PRIMER_LEFT_4_SEQUENCE=CCTGGAAGCCACATTGACTT
12494
PRIMER_RIGHT_4_SEQUENCE=TGTGGCACAAAAGCCTACAT
12495
PRIMER_LEFT_4=25,20
12496
PRIMER_RIGHT_4=176,20
12497
PRIMER_LEFT_4_TM=60.111
12498
PRIMER_RIGHT_4_TM=59.199
12499
PRIMER_LEFT_4_GC_PERCENT=50.000
12500
PRIMER_RIGHT_4_GC_PERCENT=45.000
12501
PRIMER_LEFT_4_SELF_ANY=6.00
12502
PRIMER_RIGHT_4_SELF_ANY=7.00
12503
PRIMER_LEFT_4_SELF_END=6.00
12504
PRIMER_RIGHT_4_SELF_END=2.00
12505
PRIMER_LEFT_4_END_STABILITY=6.4000
12506
PRIMER_RIGHT_4_END_STABILITY=5.6000
12507
PRIMER_PAIR_4_COMPL_ANY=6.00
12508
PRIMER_PAIR_4_COMPL_END=2.00
12509
PRIMER_PRODUCT_SIZE_4=152
12510
=
12511
MARKER_NAME=13.RHAP131FE7.seq
12512
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNCCTGCTTTTCAAATGTGGAGCCTACTCTAACTTTCAGTCCCTGGGTCTCTCTCAAATATTTTCNNNNNNNNNNNNNNNNNNNNNNGTTTTTNNNNNNNNNNNNNNNNNNNNNNNGNNTNNNNCTCTCNNNNNNNCNCNCNNNNNNNNNANNTCNCTCTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANATAGNNNAAANAANCAANAGANCCCNAGAAAAAGTGCACATCNATGCCAACATACAGNAAAAAAAACATTTNGACACACANAAAGAATANAAAACANANNTGTGNNNNATCAANGGTATTTTNGAACCNAACACGAATGNGTGANCANNGNNCANNANCGNTGCTCCNNTGTGAAATTGTTANCAGCTCANTNTTCCTGCNGTAATAATANNTACCCGCAGAATNNTNTACAAAACATGGGGGNCGNAAACATATGNGTNTTACANNCNGGNGTGGCTNNNGNGTGAGCTNACANANNNNNGGNGGGGNAGCGCTCTCNGGNCANCTGCCTNNNGGNANNNNCANCGCGCCNGNANATGNAGNANNCGAANNCGCGNNGGNANNNNNTGTCTNNNAANAGGNANAAGGGGANNNCTTNTTTCCCANAGNGGCNGGCNNNANANNTNNNNNACCGGCCACACTGANNTCCCAAANNNC
12513
INCLUDED_REGION=27,677
12514
TARGET=90,22,CT
12515
TARGET=118,22,TC
12516
TARGET=189,36,GT
12517
PRIMER_COMMENT=CL is
12518
PRIMER_COMMENT=CR is
12519
=
12520
MARKER_NAME=14.RHAP131FE8.seq
12521
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNCCAGGAACCCAGCCAGTTTTCAAAGAATACTTACCATGCCATACAAGTCAGAACCTGAACCTAGGCTTTCAAGCTGTGTAAATCAATGTTCAATTGACTGTAGTAGGAGACCCACAGACTCTATTAGAAGCATTGTAGGACAATGCTAGATTTTCTGTTCTAATGTTCTTCAGATTTAGAACAACGATGACAACAGCATTAGTGTAATAGTGTTAACTGAGTACTGTAGTGCAAGAGTGCCTGCATTCCCACATACAGTATTCATAGCTTTTGGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCCAGTTTAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
12522
INCLUDED_REGION=25,321
12523
TARGET=301,32,TG
12524
PRIMER_COMMENT=CL is
12525
PRIMER_COMMENT=CR is
12526
=
12527
MARKER_NAME=15.RHAP131FE3.seq
12528
SEQUENCE=NNNNNNNNNNNNCCTGTTTGACAGCAGGCAGAAGCTCAGGAACAATTTGTGTGGGAGTCCCAGAGGGGAGCAGGGTGACTGAAAATCCTCTGGGCTGTTACAGAACGGAGTTTGGAATCAAAGTAACCAGAACATTCAGCTCAGTAAGAGCTTAAAAGGACATTTCATCCCCCTTAAAGACAGCTAATAAAAATAGATTTGCAGTCAGTTTTCATTAGCCAAAGTGAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCGCGCGCCCCGCGTGCATAGGGTGTCTTCCTCAGCTGCTCTGCTCACCTTATGTTTTGAGACAGTGTCTGTCTTTGAACCTGTAGTTCAGCTTTTTCTACTAGGCTGGCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
12529
INCLUDED_REGION=12,368
12530
TARGET=229,38,GT
12531
PRIMER_COMMENT=CL is AAAAGGACATTTCATCCCCC
12532
PRIMER_COMMENT=CR is AGGAAGACACCCTATGCACG
12533
PRIMER_PAIR_PENALTY=0.1380
12534
PRIMER_LEFT_PENALTY=0.004242
12535
PRIMER_RIGHT_PENALTY=0.133741
12536
PRIMER_LEFT_SEQUENCE=AAAAGGACATTTCATCCCCC
12537
PRIMER_RIGHT_SEQUENCE=AGGAAGACACCCTATGCACG
12538
PRIMER_LEFT=153,20
12539
PRIMER_RIGHT=298,20
12540
PRIMER_LEFT_TM=59.996
12541
PRIMER_RIGHT_TM=60.134
12542
PRIMER_LEFT_GC_PERCENT=45.000
12543
PRIMER_RIGHT_GC_PERCENT=55.000
12544
PRIMER_LEFT_SELF_ANY=5.00
12545
PRIMER_RIGHT_SELF_ANY=4.00
12546
PRIMER_LEFT_SELF_END=0.00
12547
PRIMER_RIGHT_SELF_END=2.00
12548
PRIMER_LEFT_END_STABILITY=12.4000
12549
PRIMER_RIGHT_END_STABILITY=9.9000
12550
PRIMER_PAIR_COMPL_ANY=4.00
12551
PRIMER_PAIR_COMPL_END=1.00
12552
PRIMER_PRODUCT_SIZE=146
12553
PRIMER_PAIR_PENALTY_1=0.1476
12554
PRIMER_LEFT_1_PENALTY=0.004242
12555
PRIMER_RIGHT_1_PENALTY=0.143403
12556
PRIMER_LEFT_1_SEQUENCE=AAAAGGACATTTCATCCCCC
12557
PRIMER_RIGHT_1_SEQUENCE=GAGCAGCTGAGGAAGACACC
12558
PRIMER_LEFT_1=153,20
12559
PRIMER_RIGHT_1=307,20
12560
PRIMER_LEFT_1_TM=59.996
12561
PRIMER_RIGHT_1_TM=60.143
12562
PRIMER_LEFT_1_GC_PERCENT=45.000
12563
PRIMER_RIGHT_1_GC_PERCENT=60.000
12564
PRIMER_LEFT_1_SELF_ANY=5.00
12565
PRIMER_RIGHT_1_SELF_ANY=6.00
12566
PRIMER_LEFT_1_SELF_END=0.00
12567
PRIMER_RIGHT_1_SELF_END=0.00
12568
PRIMER_LEFT_1_END_STABILITY=12.4000
12569
PRIMER_RIGHT_1_END_STABILITY=7.6000
12570
PRIMER_PAIR_1_COMPL_ANY=4.00
12571
PRIMER_PAIR_1_COMPL_END=1.00
12572
PRIMER_PRODUCT_SIZE_1=155
12573
PRIMER_PAIR_PENALTY_2=0.4444
12574
PRIMER_LEFT_2_PENALTY=0.004242
12575
PRIMER_RIGHT_2_PENALTY=0.440146
12576
PRIMER_LEFT_2_SEQUENCE=AAAAGGACATTTCATCCCCC
12577
PRIMER_RIGHT_2_SEQUENCE=GCAGAGCAGCTGAGGAAGAC
12578
PRIMER_LEFT_2=153,20
12579
PRIMER_RIGHT_2=310,20
12580
PRIMER_LEFT_2_TM=59.996
12581
PRIMER_RIGHT_2_TM=60.440
12582
PRIMER_LEFT_2_GC_PERCENT=45.000
12583
PRIMER_RIGHT_2_GC_PERCENT=60.000
12584
PRIMER_LEFT_2_SELF_ANY=5.00
12585
PRIMER_RIGHT_2_SELF_ANY=6.00
12586
PRIMER_LEFT_2_SELF_END=0.00
12587
PRIMER_RIGHT_2_SELF_END=1.00
12588
PRIMER_LEFT_2_END_STABILITY=12.4000
12589
PRIMER_RIGHT_2_END_STABILITY=6.4000
12590
PRIMER_PAIR_2_COMPL_ANY=4.00
12591
PRIMER_PAIR_2_COMPL_END=2.00
12592
PRIMER_PRODUCT_SIZE_2=158
12593
PRIMER_PAIR_PENALTY_3=0.5051
12594
PRIMER_LEFT_3_PENALTY=0.371364
12595
PRIMER_RIGHT_3_PENALTY=0.133741
12596
PRIMER_LEFT_3_SEQUENCE=AAGGACATTTCATCCCCCTT
12597
PRIMER_RIGHT_3_SEQUENCE=AGGAAGACACCCTATGCACG
12598
PRIMER_LEFT_3=155,20
12599
PRIMER_RIGHT_3=298,20
12600
PRIMER_LEFT_3_TM=59.629
12601
PRIMER_RIGHT_3_TM=60.134
12602
PRIMER_LEFT_3_GC_PERCENT=45.000
12603
PRIMER_RIGHT_3_GC_PERCENT=55.000
12604
PRIMER_LEFT_3_SELF_ANY=4.00
12605
PRIMER_RIGHT_3_SELF_ANY=4.00
12606
PRIMER_LEFT_3_SELF_END=1.00
12607
PRIMER_RIGHT_3_SELF_END=2.00
12608
PRIMER_LEFT_3_END_STABILITY=9.7000
12609
PRIMER_RIGHT_3_END_STABILITY=9.9000
12610
PRIMER_PAIR_3_COMPL_ANY=4.00
12611
PRIMER_PAIR_3_COMPL_END=1.00
12612
PRIMER_PRODUCT_SIZE_3=144
12613
PRIMER_PAIR_PENALTY_4=0.5051
12614
PRIMER_LEFT_4_PENALTY=0.371364
12615
PRIMER_RIGHT_4_PENALTY=0.133741
12616
PRIMER_LEFT_4_SEQUENCE=AAAGGACATTTCATCCCCCT
12617
PRIMER_RIGHT_4_SEQUENCE=AGGAAGACACCCTATGCACG
12618
PRIMER_LEFT_4=154,20
12619
PRIMER_RIGHT_4=298,20
12620
PRIMER_LEFT_4_TM=59.629
12621
PRIMER_RIGHT_4_TM=60.134
12622
PRIMER_LEFT_4_GC_PERCENT=45.000
12623
PRIMER_RIGHT_4_GC_PERCENT=55.000
12624
PRIMER_LEFT_4_SELF_ANY=5.00
12625
PRIMER_RIGHT_4_SELF_ANY=4.00
12626
PRIMER_LEFT_4_SELF_END=1.00
12627
PRIMER_RIGHT_4_SELF_END=2.00
12628
PRIMER_LEFT_4_END_STABILITY=10.9000
12629
PRIMER_RIGHT_4_END_STABILITY=9.9000
12630
PRIMER_PAIR_4_COMPL_ANY=4.00
12631
PRIMER_PAIR_4_COMPL_END=1.00
12632
PRIMER_PRODUCT_SIZE_4=145
12633
=
12634
MARKER_NAME=15.RHAP131FE9.seq
12635
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNCCTTTCCATTGTGAGACACTCATTGTTGGACTAGCCCAACCATAGCCTGCAAGCCATTCTAATAAAAATTGCTTTAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCATGAGCAGGAGGATGGAAACATGATGCCCTTAATTCAATGAAGTCTAAACAATATATAGACAGTTGCTATCAAGCTCTAGGAAAACAATCAGCACGACTTATTTGGGAATAAATAGATCACAAGAGTCTCTTGAGTCAACTTACAAGAGTCCCTAGCCCATGTTTCATGGATGCTGCACTCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
12636
INCLUDED_REGION=26,309
12637
TARGET=104,46,GT
12638
PRIMER_COMMENT=CL is TGGACTAGCCCAACCATAGC
12639
PRIMER_COMMENT=CR is AAGGGCATCATGTTTCCATC
12640
PRIMER_PAIR_PENALTY=0.3396
12641
PRIMER_LEFT_PENALTY=0.095771
12642
PRIMER_RIGHT_PENALTY=0.243800
12643
PRIMER_LEFT_SEQUENCE=TGGACTAGCCCAACCATAGC
12644
PRIMER_RIGHT_SEQUENCE=AAGGGCATCATGTTTCCATC
12645
PRIMER_LEFT=52,20
12646
PRIMER_RIGHT=183,20
12647
PRIMER_LEFT_TM=60.096
12648
PRIMER_RIGHT_TM=59.756
12649
PRIMER_LEFT_GC_PERCENT=55.000
12650
PRIMER_RIGHT_GC_PERCENT=45.000
12651
PRIMER_LEFT_SELF_ANY=8.00
12652
PRIMER_RIGHT_SELF_ANY=6.00
12653
PRIMER_LEFT_SELF_END=2.00
12654
PRIMER_RIGHT_SELF_END=4.00
12655
PRIMER_LEFT_END_STABILITY=7.1000
12656
PRIMER_RIGHT_END_STABILITY=8.1000
12657
PRIMER_PAIR_COMPL_ANY=4.00
12658
PRIMER_PAIR_COMPL_END=0.00
12659
PRIMER_PRODUCT_SIZE=132
12660
PRIMER_PAIR_PENALTY_1=0.3852
12661
PRIMER_LEFT_1_PENALTY=0.095771
12662
PRIMER_RIGHT_1_PENALTY=0.289438
12663
PRIMER_LEFT_1_SEQUENCE=TGGACTAGCCCAACCATAGC
12664
PRIMER_RIGHT_1_SEQUENCE=GCATCATGTTTCCATCCTCC
12665
PRIMER_LEFT_1=52,20
12666
PRIMER_RIGHT_1=179,20
12667
PRIMER_LEFT_1_TM=60.096
12668
PRIMER_RIGHT_1_TM=60.289
12669
PRIMER_LEFT_1_GC_PERCENT=55.000
12670
PRIMER_RIGHT_1_GC_PERCENT=50.000
12671
PRIMER_LEFT_1_SELF_ANY=8.00
12672
PRIMER_RIGHT_1_SELF_ANY=5.00
12673
PRIMER_LEFT_1_SELF_END=2.00
12674
PRIMER_RIGHT_1_SELF_END=0.00
12675
PRIMER_LEFT_1_END_STABILITY=7.1000
12676
PRIMER_RIGHT_1_END_STABILITY=9.4000
12677
PRIMER_PAIR_1_COMPL_ANY=4.00
12678
PRIMER_PAIR_1_COMPL_END=0.00
12679
PRIMER_PRODUCT_SIZE_1=128
12680
PRIMER_PAIR_PENALTY_2=0.3852
12681
PRIMER_LEFT_2_PENALTY=0.095771
12682
PRIMER_RIGHT_2_PENALTY=0.289438
12683
PRIMER_LEFT_2_SEQUENCE=TGGACTAGCCCAACCATAGC
12684
PRIMER_RIGHT_2_SEQUENCE=GGCATCATGTTTCCATCCTC
12685
PRIMER_LEFT_2=52,20
12686
PRIMER_RIGHT_2=180,20
12687
PRIMER_LEFT_2_TM=60.096
12688
PRIMER_RIGHT_2_TM=60.289
12689
PRIMER_LEFT_2_GC_PERCENT=55.000
12690
PRIMER_RIGHT_2_GC_PERCENT=50.000
12691
PRIMER_LEFT_2_SELF_ANY=8.00
12692
PRIMER_RIGHT_2_SELF_ANY=6.00
12693
PRIMER_LEFT_2_SELF_END=2.00
12694
PRIMER_RIGHT_2_SELF_END=0.00
12695
PRIMER_LEFT_2_END_STABILITY=7.1000
12696
PRIMER_RIGHT_2_END_STABILITY=7.9000
12697
PRIMER_PAIR_2_COMPL_ANY=4.00
12698
PRIMER_PAIR_2_COMPL_END=0.00
12699
PRIMER_PRODUCT_SIZE_2=129
12700
PRIMER_PAIR_PENALTY_3=0.4480
12701
PRIMER_LEFT_3_PENALTY=0.375090
12702
PRIMER_RIGHT_3_PENALTY=0.072951
12703
PRIMER_LEFT_3_SEQUENCE=TGTTGGACTAGCCCAACCAT
12704
PRIMER_RIGHT_3_SEQUENCE=CATGTTTCCATCCTCCTGCT
12705
PRIMER_LEFT_3=49,20
12706
PRIMER_RIGHT_3=175,20
12707
PRIMER_LEFT_3_TM=60.375
12708
PRIMER_RIGHT_3_TM=60.073
12709
PRIMER_LEFT_3_GC_PERCENT=50.000
12710
PRIMER_RIGHT_3_GC_PERCENT=50.000
12711
PRIMER_LEFT_3_SELF_ANY=12.00
12712
PRIMER_RIGHT_3_SELF_ANY=4.00
12713
PRIMER_LEFT_3_SELF_END=6.00
12714
PRIMER_RIGHT_3_SELF_END=0.00
12715
PRIMER_LEFT_3_END_STABILITY=7.8000
12716
PRIMER_RIGHT_3_END_STABILITY=8.2000
12717
PRIMER_PAIR_3_COMPL_ANY=4.00
12718
PRIMER_PAIR_3_COMPL_END=1.00
12719
PRIMER_PRODUCT_SIZE_3=127
12720
PRIMER_PAIR_PENALTY_4=0.6189
12721
PRIMER_LEFT_4_PENALTY=0.375090
12722
PRIMER_RIGHT_4_PENALTY=0.243800
12723
PRIMER_LEFT_4_SEQUENCE=TGTTGGACTAGCCCAACCAT
12724
PRIMER_RIGHT_4_SEQUENCE=AAGGGCATCATGTTTCCATC
12725
PRIMER_LEFT_4=49,20
12726
PRIMER_RIGHT_4=183,20
12727
PRIMER_LEFT_4_TM=60.375
12728
PRIMER_RIGHT_4_TM=59.756
12729
PRIMER_LEFT_4_GC_PERCENT=50.000
12730
PRIMER_RIGHT_4_GC_PERCENT=45.000
12731
PRIMER_LEFT_4_SELF_ANY=12.00
12732
PRIMER_RIGHT_4_SELF_ANY=6.00
12733
PRIMER_LEFT_4_SELF_END=6.00
12734
PRIMER_RIGHT_4_SELF_END=4.00
12735
PRIMER_LEFT_4_END_STABILITY=7.8000
12736
PRIMER_RIGHT_4_END_STABILITY=8.1000
12737
PRIMER_PAIR_4_COMPL_ANY=4.00
12738
PRIMER_PAIR_4_COMPL_END=1.00
12739
PRIMER_PRODUCT_SIZE_4=135
12740
=
12741
MARKER_NAME=16.RHAP131FF1.seq
12742
SEQUENCE=NNNNNNNNNNNCCACGAATCAAAGAAAAGGCAAACTAGGGAAAGAAAAGCTAGTCTCAAACACCTGACCAATGGAAACCTTAGAAAGAGTGAGAGCCAAAATGATGCAGTTCCCAGTACTCCCTTATTGAAGATGATCTAGTTCCAAGCACTCACTACTCATAAATACAGTACCTGGTTCTCCCCTATCCAATGTAATACAGTACTTAGTACTCCCCCACCCAGTGATATAGTGCTTAGTACTCAGCCATGATGAGAGTACCTAACACTTCCCCCACCCAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTGNGGGGGTGNNTNTGTNNGNNTCNGGGGGTNNNGCGCGCNNNNNNGNGNAGCATGGNGNNANNNGTCTNNTGATGTGAAANNNTATNNNCGCACAATNNCTGCANAACANANGAGNNGGNGNGNNAAATGNGGTGAAANCNGNGGNGCCNANTGAGNGAGNNNCACACANTAANANNGNGGNGCNNNNNGGCGCNNTGNANNNGGGGNANNNNNNGTGNNANCTGNACTGNNNGNNNGGCNTNNNGGNNGGNG
12743
INCLUDED_REGION=11,569
12744
TARGET=283,40,TG
12745
PRIMER_COMMENT=CL is
12746
PRIMER_COMMENT=CR is
12747
=
12748
MARKER_NAME=16.RHAP131FF7.seq
12749
SEQUENCE=NNNNNNNNNNNNCCCCTTAAATGCATCACGAGAATTCTCACTTGAGATGATTTCAATTCTAGCTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCTTGTTCCGGCGCGCACACACGCACATATACAGCAAAATATAACTGAATGCCAAAAAGCATTGTCATGTCCTGTGAAGGGCACAATTGTAATCACACAGGTATGAAAAGCTTAAAATCCTTCCATTACCTTCCATGCCTGAACACTGACAGGGTCGGGCTCAGGAGCAGAGCCCACTTCCTGAGCTATTTATTTGTATCTCTGAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
12750
INCLUDED_REGION=12,305
12751
TARGET=66,44,GT
12752
PRIMER_COMMENT=CL is CCCCTTAAATGCATCACGAG
12753
PRIMER_COMMENT=CR is TGTATATGTGCGTGTGTGCG
12754
PRIMER_PAIR_PENALTY=0.6688
12755
PRIMER_LEFT_PENALTY=0.460175
12756
PRIMER_RIGHT_PENALTY=0.208639
12757
PRIMER_LEFT_SEQUENCE=CCCCTTAAATGCATCACGAG
12758
PRIMER_RIGHT_SEQUENCE=TGTATATGTGCGTGTGTGCG
12759
PRIMER_LEFT=12,20
12760
PRIMER_RIGHT=142,20
12761
PRIMER_LEFT_TM=60.460
12762
PRIMER_RIGHT_TM=60.209
12763
PRIMER_LEFT_GC_PERCENT=50.000
12764
PRIMER_RIGHT_GC_PERCENT=50.000
12765
PRIMER_LEFT_SELF_ANY=6.00
12766
PRIMER_RIGHT_SELF_ANY=4.00
12767
PRIMER_LEFT_SELF_END=2.00
12768
PRIMER_RIGHT_SELF_END=2.00
12769
PRIMER_LEFT_END_STABILITY=8.1000
12770
PRIMER_RIGHT_END_STABILITY=9.9000
12771
PRIMER_PAIR_COMPL_ANY=4.00
12772
PRIMER_PAIR_COMPL_END=2.00
12773
PRIMER_PRODUCT_SIZE=131
12774
PRIMER_PAIR_PENALTY_1=1.1042
12775
PRIMER_LEFT_1_PENALTY=0.460175
12776
PRIMER_RIGHT_1_PENALTY=0.643998
12777
PRIMER_LEFT_1_SEQUENCE=CCCCTTAAATGCATCACGAG
12778
PRIMER_RIGHT_1_SEQUENCE=CATGACAATGCTTTTTGGCA
12779
PRIMER_LEFT_1=12,20
12780
PRIMER_RIGHT_1=178,20
12781
PRIMER_LEFT_1_TM=60.460
12782
PRIMER_RIGHT_1_TM=60.644
12783
PRIMER_LEFT_1_GC_PERCENT=50.000
12784
PRIMER_RIGHT_1_GC_PERCENT=40.000
12785
PRIMER_LEFT_1_SELF_ANY=6.00
12786
PRIMER_RIGHT_1_SELF_ANY=4.00
12787
PRIMER_LEFT_1_SELF_END=2.00
12788
PRIMER_RIGHT_1_SELF_END=3.00
12789
PRIMER_LEFT_1_END_STABILITY=8.1000
12790
PRIMER_RIGHT_1_END_STABILITY=10.0000
12791
PRIMER_PAIR_1_COMPL_ANY=6.00
12792
PRIMER_PAIR_1_COMPL_END=0.00
12793
PRIMER_PRODUCT_SIZE_1=167
12794
PRIMER_PAIR_PENALTY_2=1.1592
12795
PRIMER_LEFT_2_PENALTY=0.460175
12796
PRIMER_RIGHT_2_PENALTY=0.698990
12797
PRIMER_LEFT_2_SEQUENCE=CCCCTTAAATGCATCACGAG
12798
PRIMER_RIGHT_2_SEQUENCE=CAATGCTTTTTGGCATTCAG
12799
PRIMER_LEFT_2=12,20
12800
PRIMER_RIGHT_2=173,20
12801
PRIMER_LEFT_2_TM=60.460
12802
PRIMER_RIGHT_2_TM=59.301
12803
PRIMER_LEFT_2_GC_PERCENT=50.000
12804
PRIMER_RIGHT_2_GC_PERCENT=40.000
12805
PRIMER_LEFT_2_SELF_ANY=6.00
12806
PRIMER_RIGHT_2_SELF_ANY=5.00
12807
PRIMER_LEFT_2_SELF_END=2.00
12808
PRIMER_RIGHT_2_SELF_END=1.00
12809
PRIMER_LEFT_2_END_STABILITY=8.1000
12810
PRIMER_RIGHT_2_END_STABILITY=7.0000
12811
PRIMER_PAIR_2_COMPL_ANY=6.00
12812
PRIMER_PAIR_2_COMPL_END=1.00
12813
PRIMER_PRODUCT_SIZE_2=162
12814
PRIMER_PAIR_PENALTY_3=1.2900
12815
PRIMER_LEFT_3_PENALTY=1.081398
12816
PRIMER_RIGHT_3_PENALTY=0.208639
12817
PRIMER_LEFT_3_SEQUENCE=CCCTTAAATGCATCACGAGAA
12818
PRIMER_RIGHT_3_SEQUENCE=TGTATATGTGCGTGTGTGCG
12819
PRIMER_LEFT_3=13,21
12820
PRIMER_RIGHT_3=142,20
12821
PRIMER_LEFT_3_TM=60.081
12822
PRIMER_RIGHT_3_TM=60.209
12823
PRIMER_LEFT_3_GC_PERCENT=42.857
12824
PRIMER_RIGHT_3_GC_PERCENT=50.000
12825
PRIMER_LEFT_3_SELF_ANY=6.00
12826
PRIMER_RIGHT_3_SELF_ANY=4.00
12827
PRIMER_LEFT_3_SELF_END=2.00
12828
PRIMER_RIGHT_3_SELF_END=2.00
12829
PRIMER_LEFT_3_END_STABILITY=6.7000
12830
PRIMER_RIGHT_3_END_STABILITY=9.9000
12831
PRIMER_PAIR_3_COMPL_ANY=4.00
12832
PRIMER_PAIR_3_COMPL_END=2.00
12833
PRIMER_PRODUCT_SIZE_3=130
12834
PRIMER_PAIR_PENALTY_4=1.2991
12835
PRIMER_LEFT_4_PENALTY=0.460175
12836
PRIMER_RIGHT_4_PENALTY=0.838878
12837
PRIMER_LEFT_4_SEQUENCE=CCCCTTAAATGCATCACGAG
12838
PRIMER_RIGHT_4_SEQUENCE=ACAATGCTTTTTGGCATTCA
12839
PRIMER_LEFT_4=12,20
12840
PRIMER_RIGHT_4=174,20
12841
PRIMER_LEFT_4_TM=60.460
12842
PRIMER_RIGHT_4_TM=59.161
12843
PRIMER_LEFT_4_GC_PERCENT=50.000
12844
PRIMER_RIGHT_4_GC_PERCENT=35.000
12845
PRIMER_LEFT_4_SELF_ANY=6.00
12846
PRIMER_RIGHT_4_SELF_ANY=5.00
12847
PRIMER_LEFT_4_SELF_END=2.00
12848
PRIMER_RIGHT_4_SELF_END=1.00
12849
PRIMER_LEFT_4_END_STABILITY=8.1000
12850
PRIMER_RIGHT_4_END_STABILITY=6.9000
12851
PRIMER_PAIR_4_COMPL_ANY=6.00
12852
PRIMER_PAIR_4_COMPL_END=2.00
12853
PRIMER_PRODUCT_SIZE_4=163
12854
=
12855
MARKER_NAME=18.RHAP131FF9.seq
12856
SEQUENCE=NNNNNNNNNNNNNNNNCCGGTTGCATGCATGTACCCGCCCAGGAGCTAAGTCTGATTTTCGGATAAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTAGCTCATTTGAAAACTAGCTTATTGGCTCTGAGGCTGGGCTCTTCTAAGCCTTCCACAGCTAGTGTGCCCTGAGCTTCACTCCTAGCTCAATCCCTCAGTTTAGTTGCCTAGTTACCTTCTGTGAAGCCCATTGGTCTTGTTACCCAAGATGCTTTGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
12857
INCLUDED_REGION=16,274
12858
TARGET=69,58,AC
12859
PRIMER_COMMENT=CL is GCCCAGGAGCTAAGTCTGATT
12860
PRIMER_COMMENT=CR is CCAGCCTCAGAGCCAATAAG
12861
PRIMER_PAIR_PENALTY=1.1668
12862
PRIMER_LEFT_PENALTY=1.137562
12863
PRIMER_RIGHT_PENALTY=0.029253
12864
PRIMER_LEFT_SEQUENCE=GCCCAGGAGCTAAGTCTGATT
12865
PRIMER_RIGHT_SEQUENCE=CCAGCCTCAGAGCCAATAAG
12866
PRIMER_LEFT=36,21
12867
PRIMER_RIGHT=167,20
12868
PRIMER_LEFT_TM=59.862
12869
PRIMER_RIGHT_TM=59.971
12870
PRIMER_LEFT_GC_PERCENT=52.381
12871
PRIMER_RIGHT_GC_PERCENT=55.000
12872
PRIMER_LEFT_SELF_ANY=4.00
12873
PRIMER_RIGHT_SELF_ANY=5.00
12874
PRIMER_LEFT_SELF_END=3.00
12875
PRIMER_RIGHT_SELF_END=1.00
12876
PRIMER_LEFT_END_STABILITY=6.9000
12877
PRIMER_RIGHT_END_STABILITY=5.9000
12878
PRIMER_PAIR_COMPL_ANY=5.00
12879
PRIMER_PAIR_COMPL_END=4.00
12880
PRIMER_PRODUCT_SIZE=132
12881
PRIMER_PAIR_PENALTY_1=1.1944
12882
PRIMER_LEFT_1_PENALTY=1.137562
12883
PRIMER_RIGHT_1_PENALTY=0.056887
12884
PRIMER_LEFT_1_SEQUENCE=GCCCAGGAGCTAAGTCTGATT
12885
PRIMER_RIGHT_1_SEQUENCE=AGTGAAGCTCAGGGCACACT
12886
PRIMER_LEFT_1=36,21
12887
PRIMER_RIGHT_1=210,20
12888
PRIMER_LEFT_1_TM=59.862
12889
PRIMER_RIGHT_1_TM=60.057
12890
PRIMER_LEFT_1_GC_PERCENT=52.381
12891
PRIMER_RIGHT_1_GC_PERCENT=55.000
12892
PRIMER_LEFT_1_SELF_ANY=4.00
12893
PRIMER_RIGHT_1_SELF_ANY=5.00
12894
PRIMER_LEFT_1_SELF_END=3.00
12895
PRIMER_RIGHT_1_SELF_END=5.00
12896
PRIMER_LEFT_1_END_STABILITY=6.9000
12897
PRIMER_RIGHT_1_END_STABILITY=6.1000
12898
PRIMER_PAIR_1_COMPL_ANY=6.00
12899
PRIMER_PAIR_1_COMPL_END=1.00
12900
PRIMER_PRODUCT_SIZE_1=175
12901
PRIMER_PAIR_PENALTY_2=1.2541
12902
PRIMER_LEFT_2_PENALTY=1.137562
12903
PRIMER_RIGHT_2_PENALTY=0.116582
12904
PRIMER_LEFT_2_SEQUENCE=GCCCAGGAGCTAAGTCTGATT
12905
PRIMER_RIGHT_2_SEQUENCE=AAGGCTTAGAAGAGCCCAGC
12906
PRIMER_LEFT_2=36,21
12907
PRIMER_RIGHT_2=182,20
12908
PRIMER_LEFT_2_TM=59.862
12909
PRIMER_RIGHT_2_TM=60.117
12910
PRIMER_LEFT_2_GC_PERCENT=52.381
12911
PRIMER_RIGHT_2_GC_PERCENT=55.000
12912
PRIMER_LEFT_2_SELF_ANY=4.00
12913
PRIMER_RIGHT_2_SELF_ANY=5.00
12914
PRIMER_LEFT_2_SELF_END=3.00
12915
PRIMER_RIGHT_2_SELF_END=2.00
12916
PRIMER_LEFT_2_END_STABILITY=6.9000
12917
PRIMER_RIGHT_2_END_STABILITY=9.7000
12918
PRIMER_PAIR_2_COMPL_ANY=6.00
12919
PRIMER_PAIR_2_COMPL_END=1.00
12920
PRIMER_PRODUCT_SIZE_2=147
12921
PRIMER_PAIR_PENALTY_3=1.2554
12922
PRIMER_LEFT_3_PENALTY=1.137562
12923
PRIMER_RIGHT_3_PENALTY=0.117817
12924
PRIMER_LEFT_3_SEQUENCE=GCCCAGGAGCTAAGTCTGATT
12925
PRIMER_RIGHT_3_SEQUENCE=CAGCCTCAGAGCCAATAAGC
12926
PRIMER_LEFT_3=36,21
12927
PRIMER_RIGHT_3=166,20
12928
PRIMER_LEFT_3_TM=59.862
12929
PRIMER_RIGHT_3_TM=60.118
12930
PRIMER_LEFT_3_GC_PERCENT=52.381
12931
PRIMER_RIGHT_3_GC_PERCENT=55.000
12932
PRIMER_LEFT_3_SELF_ANY=4.00
12933
PRIMER_RIGHT_3_SELF_ANY=5.00
12934
PRIMER_LEFT_3_SELF_END=3.00
12935
PRIMER_RIGHT_3_SELF_END=2.00
12936
PRIMER_LEFT_3_END_STABILITY=6.9000
12937
PRIMER_RIGHT_3_END_STABILITY=7.5000
12938
PRIMER_PAIR_3_COMPL_ANY=5.00
12939
PRIMER_PAIR_3_COMPL_END=3.00
12940
PRIMER_PRODUCT_SIZE_3=131
12941
PRIMER_PAIR_PENALTY_4=1.3017
12942
PRIMER_LEFT_4_PENALTY=1.137562
12943
PRIMER_RIGHT_4_PENALTY=0.164128
12944
PRIMER_LEFT_4_SEQUENCE=GCCCAGGAGCTAAGTCTGATT
12945
PRIMER_RIGHT_4_SEQUENCE=GAAGCTCAGGGCACACTAGC
12946
PRIMER_LEFT_4=36,21
12947
PRIMER_RIGHT_4=207,20
12948
PRIMER_LEFT_4_TM=59.862
12949
PRIMER_RIGHT_4_TM=60.164
12950
PRIMER_LEFT_4_GC_PERCENT=52.381
12951
PRIMER_RIGHT_4_GC_PERCENT=60.000
12952
PRIMER_LEFT_4_SELF_ANY=4.00
12953
PRIMER_RIGHT_4_SELF_ANY=4.00
12954
PRIMER_LEFT_4_SELF_END=3.00
12955
PRIMER_RIGHT_4_SELF_END=4.00
12956
PRIMER_LEFT_4_END_STABILITY=6.9000
12957
PRIMER_RIGHT_4_END_STABILITY=7.2000
12958
PRIMER_PAIR_4_COMPL_ANY=6.00
12959
PRIMER_PAIR_4_COMPL_END=2.00
12960
PRIMER_PRODUCT_SIZE_4=172
12961
=
12962
MARKER_NAME=19.RHAP131FG1.seq
12963
SEQUENCE=NNNNNNNNNNNCCAGGATTGATTGACGCCTTGTGATGATGTCCTCTTCTAACCCTGCTCTTATACAAGGTGGAGTAGCTGGTATGTTTGAATGTTTAAATAAAAGGTACCCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTGCTTGGTATAGGTGCTTATAACTGTATCTCAGGTCCTCTGGGTTTGACAGTCAACATAACAGCCATGACAGCCCTGTCCACTAGGGCACTTCTATCAGTTCTGATATCACAGTGGAGCCAAACATGGACTGTTAGCATTCTATGCTCCACCCAGTGTTACCTAGCAACAACCAGGCTAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
12964
INCLUDED_REGION=11,331
12965
TARGET=113,46,GT
12966
PRIMER_COMMENT=CL is CCCTGCTCTTATACAAGGTGG
12967
PRIMER_COMMENT=CR is GTCAAACCCAGAGGACCTGA
12968
PRIMER_PAIR_PENALTY=2.3586
12969
PRIMER_LEFT_PENALTY=2.270426
12970
PRIMER_RIGHT_PENALTY=0.088170
12971
PRIMER_LEFT_SEQUENCE=CCCTGCTCTTATACAAGGTGG
12972
PRIMER_RIGHT_SEQUENCE=GTCAAACCCAGAGGACCTGA
12973
PRIMER_LEFT=51,21
12974
PRIMER_RIGHT=209,20
12975
PRIMER_LEFT_TM=58.730
12976
PRIMER_RIGHT_TM=60.088
12977
PRIMER_LEFT_GC_PERCENT=52.381
12978
PRIMER_RIGHT_GC_PERCENT=55.000
12979
PRIMER_LEFT_SELF_ANY=6.00
12980
PRIMER_RIGHT_SELF_ANY=5.00
12981
PRIMER_LEFT_SELF_END=4.00
12982
PRIMER_RIGHT_SELF_END=1.00
12983
PRIMER_LEFT_END_STABILITY=9.4000
12984
PRIMER_RIGHT_END_STABILITY=8.2000
12985
PRIMER_PAIR_COMPL_ANY=4.00
12986
PRIMER_PAIR_COMPL_END=0.00
12987
PRIMER_PRODUCT_SIZE=159
12988
PRIMER_PAIR_PENALTY_1=2.4996
12989
PRIMER_LEFT_1_PENALTY=2.270426
12990
PRIMER_RIGHT_1_PENALTY=0.229181
12991
PRIMER_LEFT_1_SEQUENCE=CCCTGCTCTTATACAAGGTGG
12992
PRIMER_RIGHT_1_SEQUENCE=CAAACCCAGAGGACCTGAGA
12993
PRIMER_LEFT_1=51,21
12994
PRIMER_RIGHT_1=207,20
12995
PRIMER_LEFT_1_TM=58.730
12996
PRIMER_RIGHT_1_TM=60.229
12997
PRIMER_LEFT_1_GC_PERCENT=52.381
12998
PRIMER_RIGHT_1_GC_PERCENT=55.000
12999
PRIMER_LEFT_1_SELF_ANY=6.00
13000
PRIMER_RIGHT_1_SELF_ANY=5.00
13001
PRIMER_LEFT_1_SELF_END=4.00
13002
PRIMER_RIGHT_1_SELF_END=1.00
13003
PRIMER_LEFT_1_END_STABILITY=9.4000
13004
PRIMER_RIGHT_1_END_STABILITY=6.7000
13005
PRIMER_PAIR_1_COMPL_ANY=4.00
13006
PRIMER_PAIR_1_COMPL_END=0.00
13007
PRIMER_PRODUCT_SIZE_1=157
13008
PRIMER_PAIR_PENALTY_2=2.4996
13009
PRIMER_LEFT_2_PENALTY=2.270426
13010
PRIMER_RIGHT_2_PENALTY=0.229181
13011
PRIMER_LEFT_2_SEQUENCE=CCCTGCTCTTATACAAGGTGG
13012
PRIMER_RIGHT_2_SEQUENCE=TCAAACCCAGAGGACCTGAG
13013
PRIMER_LEFT_2=51,21
13014
PRIMER_RIGHT_2=208,20
13015
PRIMER_LEFT_2_TM=58.730
13016
PRIMER_RIGHT_2_TM=60.229
13017
PRIMER_LEFT_2_GC_PERCENT=52.381
13018
PRIMER_RIGHT_2_GC_PERCENT=55.000
13019
PRIMER_LEFT_2_SELF_ANY=6.00
13020
PRIMER_RIGHT_2_SELF_ANY=5.00
13021
PRIMER_LEFT_2_SELF_END=4.00
13022
PRIMER_RIGHT_2_SELF_END=3.00
13023
PRIMER_LEFT_2_END_STABILITY=9.4000
13024
PRIMER_RIGHT_2_END_STABILITY=6.7000
13025
PRIMER_PAIR_2_COMPL_ANY=4.00
13026
PRIMER_PAIR_2_COMPL_END=0.00
13027
PRIMER_PRODUCT_SIZE_2=158
13028
PRIMER_PAIR_PENALTY_3=2.5451
13029
PRIMER_LEFT_3_PENALTY=2.456883
13030
PRIMER_RIGHT_3_PENALTY=0.088170
13031
PRIMER_LEFT_3_SEQUENCE=ACCCTGCTCTTATACAAGGTGG
13032
PRIMER_RIGHT_3_SEQUENCE=GTCAAACCCAGAGGACCTGA
13033
PRIMER_LEFT_3=50,22
13034
PRIMER_RIGHT_3=209,20
13035
PRIMER_LEFT_3_TM=59.543
13036
PRIMER_RIGHT_3_TM=60.088
13037
PRIMER_LEFT_3_GC_PERCENT=50.000
13038
PRIMER_RIGHT_3_GC_PERCENT=55.000
13039
PRIMER_LEFT_3_SELF_ANY=6.00
13040
PRIMER_RIGHT_3_SELF_ANY=5.00
13041
PRIMER_LEFT_3_SELF_END=4.00
13042
PRIMER_RIGHT_3_SELF_END=1.00
13043
PRIMER_LEFT_3_END_STABILITY=9.4000
13044
PRIMER_RIGHT_3_END_STABILITY=8.2000
13045
PRIMER_PAIR_3_COMPL_ANY=4.00
13046
PRIMER_PAIR_3_COMPL_END=0.00
13047
PRIMER_PRODUCT_SIZE_3=160
13048
PRIMER_PAIR_PENALTY_4=2.5508
13049
PRIMER_LEFT_4_PENALTY=2.462622
13050
PRIMER_RIGHT_4_PENALTY=0.088170
13051
PRIMER_LEFT_4_SEQUENCE=TGTCCTCTTCTAACCCTGCTC
13052
PRIMER_RIGHT_4_SEQUENCE=GTCAAACCCAGAGGACCTGA
13053
PRIMER_LEFT_4=38,21
13054
PRIMER_RIGHT_4=209,20
13055
PRIMER_LEFT_4_TM=58.537
13056
PRIMER_RIGHT_4_TM=60.088
13057
PRIMER_LEFT_4_GC_PERCENT=52.381
13058
PRIMER_RIGHT_4_GC_PERCENT=55.000
13059
PRIMER_LEFT_4_SELF_ANY=2.00
13060
PRIMER_RIGHT_4_SELF_ANY=5.00
13061
PRIMER_LEFT_4_SELF_END=0.00
13062
PRIMER_RIGHT_4_SELF_END=1.00
13063
PRIMER_LEFT_4_END_STABILITY=8.2000
13064
PRIMER_RIGHT_4_END_STABILITY=8.2000
13065
PRIMER_PAIR_4_COMPL_ANY=7.00
13066
PRIMER_PAIR_4_COMPL_END=2.00
13067
PRIMER_PRODUCT_SIZE_4=172
13068
=
13069
MARKER_NAME=19.RHAP131FG7.seq
13070
SEQUENCE=NNNNNNNNNNNNNNCCATGGATAACAGGGATGGACAACAAGGAAGAGCTACAGAGACATCCAGACAGCCATTATCTGCCTCATACTATCCCAACTGTTACACTTCCTATCCATACCTGCTGCTTTCAAAACCAAGTCTAGCCCCTTCTGAGAATTATTTAGGGGTGGGTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAGAGAACAACTTGCAGAAGAAGGCTCTTTCTTACCTTCCACCACATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
13071
INCLUDED_REGION=14,258
13072
TARGET=171,52,GT
13073
PRIMER_COMMENT=CL is CCAAGTCTAGCCCCTTCTGA
13074
PRIMER_COMMENT=CR is CCATGTGGTGGAAGGTAAGAA
13075
PRIMER_PAIR_PENALTY=1.7372
13076
PRIMER_LEFT_PENALTY=0.575355
13077
PRIMER_RIGHT_PENALTY=1.161853
13078
PRIMER_LEFT_SEQUENCE=CCAAGTCTAGCCCCTTCTGA
13079
PRIMER_RIGHT_SEQUENCE=CCATGTGGTGGAAGGTAAGAA
13080
PRIMER_LEFT=130,20
13081
PRIMER_RIGHT=271,21
13082
PRIMER_LEFT_TM=59.425
13083
PRIMER_RIGHT_TM=59.838
13084
PRIMER_LEFT_GC_PERCENT=55.000
13085
PRIMER_RIGHT_GC_PERCENT=47.619
13086
PRIMER_LEFT_SELF_ANY=4.00
13087
PRIMER_RIGHT_SELF_ANY=4.00
13088
PRIMER_LEFT_SELF_END=3.00
13089
PRIMER_RIGHT_SELF_END=0.00
13090
PRIMER_LEFT_END_STABILITY=6.7000
13091
PRIMER_RIGHT_END_STABILITY=7.0000
13092
PRIMER_PAIR_COMPL_ANY=5.00
13093
PRIMER_PAIR_COMPL_END=4.00
13094
PRIMER_PRODUCT_SIZE=142
13095
PRIMER_PAIR_PENALTY_1=1.7420
13096
PRIMER_LEFT_1_PENALTY=0.580167
13097
PRIMER_RIGHT_1_PENALTY=1.161853
13098
PRIMER_LEFT_1_SEQUENCE=TCCATACCTGCTGCTTTCAA
13099
PRIMER_RIGHT_1_SEQUENCE=CCATGTGGTGGAAGGTAAGAA
13100
PRIMER_LEFT_1=108,20
13101
PRIMER_RIGHT_1=271,21
13102
PRIMER_LEFT_1_TM=59.420
13103
PRIMER_RIGHT_1_TM=59.838
13104
PRIMER_LEFT_1_GC_PERCENT=45.000
13105
PRIMER_RIGHT_1_GC_PERCENT=47.619
13106
PRIMER_LEFT_1_SELF_ANY=3.00
13107
PRIMER_RIGHT_1_SELF_ANY=4.00
13108
PRIMER_LEFT_1_SELF_END=3.00
13109
PRIMER_RIGHT_1_SELF_END=0.00
13110
PRIMER_LEFT_1_END_STABILITY=7.3000
13111
PRIMER_RIGHT_1_END_STABILITY=7.0000
13112
PRIMER_PAIR_1_COMPL_ANY=5.00
13113
PRIMER_PAIR_1_COMPL_END=2.00
13114
PRIMER_PRODUCT_SIZE_1=164
13115
PRIMER_PAIR_PENALTY_2=1.9685
13116
PRIMER_LEFT_2_PENALTY=0.806616
13117
PRIMER_RIGHT_2_PENALTY=1.161853
13118
PRIMER_LEFT_2_SEQUENCE=ACCTGCTGCTTTCAAAACCA
13119
PRIMER_RIGHT_2_SEQUENCE=CCATGTGGTGGAAGGTAAGAA
13120
PRIMER_LEFT_2=113,20
13121
PRIMER_RIGHT_2=271,21
13122
PRIMER_LEFT_2_TM=60.807
13123
PRIMER_RIGHT_2_TM=59.838
13124
PRIMER_LEFT_2_GC_PERCENT=45.000
13125
PRIMER_RIGHT_2_GC_PERCENT=47.619
13126
PRIMER_LEFT_2_SELF_ANY=6.00
13127
PRIMER_RIGHT_2_SELF_ANY=4.00
13128
PRIMER_LEFT_2_SELF_END=6.00
13129
PRIMER_RIGHT_2_SELF_END=0.00
13130
PRIMER_LEFT_2_END_STABILITY=8.2000
13131
PRIMER_RIGHT_2_END_STABILITY=7.0000
13132
PRIMER_PAIR_2_COMPL_ANY=4.00
13133
PRIMER_PAIR_2_COMPL_END=1.00
13134
PRIMER_PRODUCT_SIZE_2=159
13135
PRIMER_PAIR_PENALTY_3=1.9914
13136
PRIMER_LEFT_3_PENALTY=0.829561
13137
PRIMER_RIGHT_3_PENALTY=1.161853
13138
PRIMER_LEFT_3_SEQUENCE=TCAAAACCAAGTCTAGCCCC
13139
PRIMER_RIGHT_3_SEQUENCE=CCATGTGGTGGAAGGTAAGAA
13140
PRIMER_LEFT_3=124,20
13141
PRIMER_RIGHT_3=271,21
13142
PRIMER_LEFT_3_TM=59.170
13143
PRIMER_RIGHT_3_TM=59.838
13144
PRIMER_LEFT_3_GC_PERCENT=50.000
13145
PRIMER_RIGHT_3_GC_PERCENT=47.619
13146
PRIMER_LEFT_3_SELF_ANY=4.00
13147
PRIMER_RIGHT_3_SELF_ANY=4.00
13148
PRIMER_LEFT_3_SELF_END=0.00
13149
PRIMER_RIGHT_3_SELF_END=0.00
13150
PRIMER_LEFT_3_END_STABILITY=12.4000
13151
PRIMER_RIGHT_3_END_STABILITY=7.0000
13152
PRIMER_PAIR_3_COMPL_ANY=4.00
13153
PRIMER_PAIR_3_COMPL_END=0.00
13154
PRIMER_PRODUCT_SIZE_3=148
13155
PRIMER_PAIR_PENALTY_4=2.1613
13156
PRIMER_LEFT_4_PENALTY=0.575355
13157
PRIMER_RIGHT_4_PENALTY=1.585973
13158
PRIMER_LEFT_4_SEQUENCE=CCAAGTCTAGCCCCTTCTGA
13159
PRIMER_RIGHT_4_SEQUENCE=CCATGTGGTGGAAGGTAAGA
13160
PRIMER_LEFT_4=130,20
13161
PRIMER_RIGHT_4=271,20
13162
PRIMER_LEFT_4_TM=59.425
13163
PRIMER_RIGHT_4_TM=58.414
13164
PRIMER_LEFT_4_GC_PERCENT=55.000
13165
PRIMER_RIGHT_4_GC_PERCENT=50.000
13166
PRIMER_LEFT_4_SELF_ANY=4.00
13167
PRIMER_RIGHT_4_SELF_ANY=4.00
13168
PRIMER_LEFT_4_SELF_END=3.00
13169
PRIMER_RIGHT_4_SELF_END=0.00
13170
PRIMER_LEFT_4_END_STABILITY=6.7000
13171
PRIMER_RIGHT_4_END_STABILITY=6.0000
13172
PRIMER_PAIR_4_COMPL_ANY=5.00
13173
PRIMER_PAIR_4_COMPL_END=3.00
13174
PRIMER_PRODUCT_SIZE_4=142
13175
=
13176
MARKER_NAME=20.RHAP131FG2.seq
13177
SEQUENCE=CCGGGTTTTTGTGTATGCTTGGGTGTGGGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTATTTGTATTTGTGTTATANNNNNNNNNNNNNTTTGCATGTGCGTGTGTGAGTGTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
13178
INCLUDED_REGION=0,149
13179
TARGET=31,60,TG
13180
TARGET=111,13,TG
13181
PRIMER_COMMENT=CL is CCGGGTTTTTGTGTATGCTT
13182
PRIMER_COMMENT=CR is GACACTCACACACGCACATG
13183
PRIMER_PAIR_PENALTY=0.3675
13184
PRIMER_LEFT_PENALTY=0.139129
13185
PRIMER_RIGHT_PENALTY=0.228420
13186
PRIMER_LEFT_SEQUENCE=CCGGGTTTTTGTGTATGCTT
13187
PRIMER_RIGHT_SEQUENCE=GACACTCACACACGCACATG
13188
PRIMER_LEFT=0,20
13189
PRIMER_RIGHT=147,20
13190
PRIMER_LEFT_TM=59.861
13191
PRIMER_RIGHT_TM=59.772
13192
PRIMER_LEFT_GC_PERCENT=45.000
13193
PRIMER_RIGHT_GC_PERCENT=55.000
13194
PRIMER_LEFT_SELF_ANY=4.00
13195
PRIMER_RIGHT_SELF_ANY=4.00
13196
PRIMER_LEFT_SELF_END=2.00
13197
PRIMER_RIGHT_SELF_END=4.00
13198
PRIMER_LEFT_END_STABILITY=8.5000
13199
PRIMER_RIGHT_END_STABILITY=6.6000
13200
PRIMER_PAIR_COMPL_ANY=5.00
13201
PRIMER_PAIR_COMPL_END=1.00
13202
PRIMER_PRODUCT_SIZE=148
13203
PRIMER_PAIR_PENALTY_1=0.3882
13204
PRIMER_LEFT_1_PENALTY=0.159750
13205
PRIMER_RIGHT_1_PENALTY=0.228420
13206
PRIMER_LEFT_1_SEQUENCE=TGTATGCTTGGGTGTGGGTA
13207
PRIMER_RIGHT_1_SEQUENCE=GACACTCACACACGCACATG
13208
PRIMER_LEFT_1=11,20
13209
PRIMER_RIGHT_1=147,20
13210
PRIMER_LEFT_1_TM=59.840
13211
PRIMER_RIGHT_1_TM=59.772
13212
PRIMER_LEFT_1_GC_PERCENT=50.000
13213
PRIMER_RIGHT_1_GC_PERCENT=55.000
13214
PRIMER_LEFT_1_SELF_ANY=2.00
13215
PRIMER_RIGHT_1_SELF_ANY=4.00
13216
PRIMER_LEFT_1_SELF_END=2.00
13217
PRIMER_RIGHT_1_SELF_END=4.00
13218
PRIMER_LEFT_1_END_STABILITY=8.4000
13219
PRIMER_RIGHT_1_END_STABILITY=6.6000
13220
PRIMER_PAIR_1_COMPL_ANY=7.00
13221
PRIMER_PAIR_1_COMPL_END=4.00
13222
PRIMER_PRODUCT_SIZE_1=137
13223
PRIMER_PAIR_PENALTY_2=0.6552
13224
PRIMER_LEFT_2_PENALTY=0.426779
13225
PRIMER_RIGHT_2_PENALTY=0.228420
13226
PRIMER_LEFT_2_SEQUENCE=TTGTGTATGCTTGGGTGTGG
13227
PRIMER_RIGHT_2_SEQUENCE=GACACTCACACACGCACATG
13228
PRIMER_LEFT_2=8,20
13229
PRIMER_RIGHT_2=147,20
13230
PRIMER_LEFT_2_TM=60.427
13231
PRIMER_RIGHT_2_TM=59.772
13232
PRIMER_LEFT_2_GC_PERCENT=50.000
13233
PRIMER_RIGHT_2_GC_PERCENT=55.000
13234
PRIMER_LEFT_2_SELF_ANY=2.00
13235
PRIMER_RIGHT_2_SELF_ANY=4.00
13236
PRIMER_LEFT_2_SELF_END=0.00
13237
PRIMER_RIGHT_2_SELF_END=4.00
13238
PRIMER_LEFT_2_END_STABILITY=8.2000
13239
PRIMER_RIGHT_2_END_STABILITY=6.6000
13240
PRIMER_PAIR_2_COMPL_ANY=7.00
13241
PRIMER_PAIR_2_COMPL_END=3.00
13242
PRIMER_PRODUCT_SIZE_2=140
13243
PRIMER_PAIR_PENALTY_3=0.9283
13244
PRIMER_LEFT_3_PENALTY=0.699863
13245
PRIMER_RIGHT_3_PENALTY=0.228420
13246
PRIMER_LEFT_3_SEQUENCE=GTGTATGCTTGGGTGTGGGT
13247
PRIMER_RIGHT_3_SEQUENCE=GACACTCACACACGCACATG
13248
PRIMER_LEFT_3=10,20
13249
PRIMER_RIGHT_3=147,20
13250
PRIMER_LEFT_3_TM=60.700
13251
PRIMER_RIGHT_3_TM=59.772
13252
PRIMER_LEFT_3_GC_PERCENT=55.000
13253
PRIMER_RIGHT_3_GC_PERCENT=55.000
13254
PRIMER_LEFT_3_SELF_ANY=2.00
13255
PRIMER_RIGHT_3_SELF_ANY=4.00
13256
PRIMER_LEFT_3_SELF_END=0.00
13257
PRIMER_RIGHT_3_SELF_END=4.00
13258
PRIMER_LEFT_3_END_STABILITY=9.4000
13259
PRIMER_RIGHT_3_END_STABILITY=6.6000
13260
PRIMER_PAIR_3_COMPL_ANY=7.00
13261
PRIMER_PAIR_3_COMPL_END=5.00
13262
PRIMER_PRODUCT_SIZE_3=138
13263
PRIMER_PAIR_PENALTY_4=1.1450
13264
PRIMER_LEFT_4_PENALTY=0.916535
13265
PRIMER_RIGHT_4_PENALTY=0.228420
13266
PRIMER_LEFT_4_SEQUENCE=CGGGTTTTTGTGTATGCTTG
13267
PRIMER_RIGHT_4_SEQUENCE=GACACTCACACACGCACATG
13268
PRIMER_LEFT_4=1,20
13269
PRIMER_RIGHT_4=147,20
13270
PRIMER_LEFT_4_TM=59.083
13271
PRIMER_RIGHT_4_TM=59.772
13272
PRIMER_LEFT_4_GC_PERCENT=45.000
13273
PRIMER_RIGHT_4_GC_PERCENT=55.000
13274
PRIMER_LEFT_4_SELF_ANY=2.00
13275
PRIMER_RIGHT_4_SELF_ANY=4.00
13276
PRIMER_LEFT_4_SELF_END=0.00
13277
PRIMER_RIGHT_4_SELF_END=4.00
13278
PRIMER_LEFT_4_END_STABILITY=8.5000
13279
PRIMER_RIGHT_4_END_STABILITY=6.6000
13280
PRIMER_PAIR_4_COMPL_ANY=5.00
13281
PRIMER_PAIR_4_COMPL_END=2.00
13282
PRIMER_PRODUCT_SIZE_4=147
13283
=
13284
MARKER_NAME=20.RHAP131FG8.seq
13285
SEQUENCE=NNNNNNNNNNNNNNNCCGTGGTGGTTCTATTTGCAGCCCTAGCATCTTCCCAGAAGAGGCGTTGAAATGAAGGGGCTACTGAGCTGGCTCAGCAGGAAAAGGCACTTAATATACAACCTGGCAATGCAAAATTCAATCCTAGGAACTCACGTGAGAGGTGGAAGGAGAGAACCAACTCCACAAACTAGTCCTCCAGCCTCCACACATGTGCCCCACCCCCAACTCACATATATAACTACGTCATTAACGTTTTTTTAAATTGTCTTAAGTGACAGCTGTGTGTCTTGACACACTCCTGTAATCCTGGCATGAGAAGCAGGAGTTCGAGGCCACAGCAGCTGACTTTGTCCATCTGCAGACCTTTTAACTCTTCAAGCTGAAGGTGTGGGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGCACCTTTCCTTGTGGATTTGGATTTGGAGTTTCCACCTGTCTTTAGTTAATACATTCCCATTGACCATTCTTTTTTAACATTGGTTGTACCAGAGACAACGGGTAGACATCATTTCCTGTGCTGCAAATAGGNGGAACTCCTAGAGCTTTATTGNCTCTGGNNTGTGNANAGNNTGNACTNGAGAACACAAAGNATGTCAATTTNTAGAATNTGACAATTTTAGNGTCNAACATGGGTTGTGT
13286
INCLUDED_REGION=15,666
13287
TARGET=391,44,AC
13288
PRIMER_COMMENT=CL is GGCATGAGAAGCAGGAGTTC
13289
PRIMER_COMMENT=CR is ATCCACAAGGAAAGGTGCAC
13290
PRIMER_PAIR_PENALTY=0.0693
13291
PRIMER_LEFT_PENALTY=0.042732
13292
PRIMER_RIGHT_PENALTY=0.026532
13293
PRIMER_LEFT_SEQUENCE=GGCATGAGAAGCAGGAGTTC
13294
PRIMER_RIGHT_SEQUENCE=ATCCACAAGGAAAGGTGCAC
13295
PRIMER_LEFT=305,20
13296
PRIMER_RIGHT=454,20
13297
PRIMER_LEFT_TM=59.957
13298
PRIMER_RIGHT_TM=59.973
13299
PRIMER_LEFT_GC_PERCENT=55.000
13300
PRIMER_RIGHT_GC_PERCENT=50.000
13301
PRIMER_LEFT_SELF_ANY=4.00
13302
PRIMER_RIGHT_SELF_ANY=6.00
13303
PRIMER_LEFT_SELF_END=2.00
13304
PRIMER_RIGHT_SELF_END=6.00
13305
PRIMER_LEFT_END_STABILITY=6.4000
13306
PRIMER_RIGHT_END_STABILITY=8.2000
13307
PRIMER_PAIR_COMPL_ANY=3.00
13308
PRIMER_PAIR_COMPL_END=2.00
13309
PRIMER_PRODUCT_SIZE=150
13310
PRIMER_PAIR_PENALTY_1=0.1396
13311
PRIMER_LEFT_1_PENALTY=0.042732
13312
PRIMER_RIGHT_1_PENALTY=0.096855
13313
PRIMER_LEFT_1_SEQUENCE=GGCATGAGAAGCAGGAGTTC
13314
PRIMER_RIGHT_1_SEQUENCE=TCCAAATCCACAAGGAAAGG
13315
PRIMER_LEFT_1=305,20
13316
PRIMER_RIGHT_1=459,20
13317
PRIMER_LEFT_1_TM=59.957
13318
PRIMER_RIGHT_1_TM=59.903
13319
PRIMER_LEFT_1_GC_PERCENT=55.000
13320
PRIMER_RIGHT_1_GC_PERCENT=45.000
13321
PRIMER_LEFT_1_SELF_ANY=4.00
13322
PRIMER_RIGHT_1_SELF_ANY=3.00
13323
PRIMER_LEFT_1_SELF_END=2.00
13324
PRIMER_RIGHT_1_SELF_END=0.00
13325
PRIMER_LEFT_1_END_STABILITY=6.4000
13326
PRIMER_RIGHT_1_END_STABILITY=8.5000
13327
PRIMER_PAIR_1_COMPL_ANY=4.00
13328
PRIMER_PAIR_1_COMPL_END=1.00
13329
PRIMER_PRODUCT_SIZE_1=155
13330
PRIMER_PAIR_PENALTY_2=0.1705
13331
PRIMER_LEFT_2_PENALTY=0.008461
13332
PRIMER_RIGHT_2_PENALTY=0.162074
13333
PRIMER_LEFT_2_SEQUENCE=GCTGACTTTGTCCATCTGCA
13334
PRIMER_RIGHT_2_SEQUENCE=CCAAATCCAAATCCACAAGG
13335
PRIMER_LEFT_2=337,20
13336
PRIMER_RIGHT_2=464,20
13337
PRIMER_LEFT_2_TM=59.992
13338
PRIMER_RIGHT_2_TM=60.162
13339
PRIMER_LEFT_2_GC_PERCENT=50.000
13340
PRIMER_RIGHT_2_GC_PERCENT=45.000
13341
PRIMER_LEFT_2_SELF_ANY=4.00
13342
PRIMER_RIGHT_2_SELF_ANY=2.00
13343
PRIMER_LEFT_2_SELF_END=4.00
13344
PRIMER_RIGHT_2_SELF_END=0.00
13345
PRIMER_LEFT_2_END_STABILITY=8.5000
13346
PRIMER_RIGHT_2_END_STABILITY=8.5000
13347
PRIMER_PAIR_2_COMPL_ANY=5.00
13348
PRIMER_PAIR_2_COMPL_END=5.00
13349
PRIMER_PRODUCT_SIZE_2=128
13350
PRIMER_PAIR_PENALTY_3=0.2048
13351
PRIMER_LEFT_3_PENALTY=0.042732
13352
PRIMER_RIGHT_3_PENALTY=0.162074
13353
PRIMER_LEFT_3_SEQUENCE=GGCATGAGAAGCAGGAGTTC
13354
PRIMER_RIGHT_3_SEQUENCE=CCAAATCCAAATCCACAAGG
13355
PRIMER_LEFT_3=305,20
13356
PRIMER_RIGHT_3=464,20
13357
PRIMER_LEFT_3_TM=59.957
13358
PRIMER_RIGHT_3_TM=60.162
13359
PRIMER_LEFT_3_GC_PERCENT=55.000
13360
PRIMER_RIGHT_3_GC_PERCENT=45.000
13361
PRIMER_LEFT_3_SELF_ANY=4.00
13362
PRIMER_RIGHT_3_SELF_ANY=2.00
13363
PRIMER_LEFT_3_SELF_END=2.00
13364
PRIMER_RIGHT_3_SELF_END=0.00
13365
PRIMER_LEFT_3_END_STABILITY=6.4000
13366
PRIMER_RIGHT_3_END_STABILITY=8.5000
13367
PRIMER_PAIR_3_COMPL_ANY=4.00
13368
PRIMER_PAIR_3_COMPL_END=1.00
13369
PRIMER_PRODUCT_SIZE_3=160
13370
PRIMER_PAIR_PENALTY_4=0.2413
13371
PRIMER_LEFT_4_PENALTY=0.008461
13372
PRIMER_RIGHT_4_PENALTY=0.232835
13373
PRIMER_LEFT_4_SEQUENCE=GCTGACTTTGTCCATCTGCA
13374
PRIMER_RIGHT_4_SEQUENCE=GGTGGAAACTCCAAATCCAA
13375
PRIMER_LEFT_4=337,20
13376
PRIMER_RIGHT_4=474,20
13377
PRIMER_LEFT_4_TM=59.992
13378
PRIMER_RIGHT_4_TM=59.767
13379
PRIMER_LEFT_4_GC_PERCENT=50.000
13380
PRIMER_RIGHT_4_GC_PERCENT=45.000
13381
PRIMER_LEFT_4_SELF_ANY=4.00
13382
PRIMER_RIGHT_4_SELF_ANY=5.00
13383
PRIMER_LEFT_4_SELF_END=4.00
13384
PRIMER_RIGHT_4_SELF_END=0.00
13385
PRIMER_LEFT_4_END_STABILITY=8.5000
13386
PRIMER_RIGHT_4_END_STABILITY=8.5000
13387
PRIMER_PAIR_4_COMPL_ANY=4.00
13388
PRIMER_PAIR_4_COMPL_END=1.00
13389
PRIMER_PRODUCT_SIZE_4=138
13390
=
13391
MARKER_NAME=23.RHAP131FH2.seq
13392
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCCTCACTTCTGCTACACAGTTAGCTTCCTTTTCAAAACCTCGTCTTCCTACTTTGGATCCCTTTCTCACCTCCTCTTCTGCTTCCCTTTTTGTTATGGTGGATTGTTCTTGGCTGTCTGTCTGCTTTATTCTTCTTGATCCCCACACCCCTTATGAGTAGCCATGAGATCTCCAGATGTCCGAGGCTTCTGTTGCTCTTTGGAGTTGACTGACACATGCCTGAACCACAGGGTTCACTGATAGCTCTCTGTGGTCCGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGCCTGCATGTATGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
13393
INCLUDED_REGION=23,322
13394
TARGET=281,46,GT
13395
PRIMER_COMMENT=CL is
13396
PRIMER_COMMENT=CR is
13397
=
13398
MARKER_NAME=23.RHAP131FH8.seq
13399
SEQUENCE=NNNNNNNNNNNNNNNCAGCATTTCCCAAGCTGGAGGAGCTANACAGACAGTAAGTAGTTTTTGGCTAATTAATTATCTTCCATAGAAAACAATGTTTCTCACAATTCGTCCTCTTTGTTAATATATTTTCAGAATGCCCCCCCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAATTCAGCCAGCATGCACTGCGTTTCTGAAATGTCTACTAATAGTAAAGTATGGCTATGATAAGTGACTGGTGACTGATGAGAGAGGTTTCCAGAGCTTCTGGACAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
13400
INCLUDED_REGION=15,285
13401
TARGET=144,46,AC
13402
PRIMER_COMMENT=CL is ATATTTTCAGAATGCCCCCC
13403
PRIMER_COMMENT=CR is TCCAGAAGCTCTGGAAACCT
13404
PRIMER_PAIR_PENALTY=1.0010
13405
PRIMER_LEFT_PENALTY=0.015502
13406
PRIMER_RIGHT_PENALTY=0.985544
13407
PRIMER_LEFT_SEQUENCE=ATATTTTCAGAATGCCCCCC
13408
PRIMER_RIGHT_SEQUENCE=CCAGAAGCTCTGGAAACCTC
13409
PRIMER_LEFT=122,20
13410
PRIMER_RIGHT=293,20
13411
PRIMER_LEFT_TM=59.984
13412
PRIMER_RIGHT_TM=59.014
13413
PRIMER_LEFT_GC_PERCENT=45.000
13414
PRIMER_RIGHT_GC_PERCENT=55.000
13415
PRIMER_LEFT_SELF_ANY=5.00
13416
PRIMER_RIGHT_SELF_ANY=8.00
13417
PRIMER_LEFT_SELF_END=0.00
13418
PRIMER_RIGHT_SELF_END=0.00
13419
PRIMER_LEFT_END_STABILITY=12.4000
13420
PRIMER_RIGHT_END_STABILITY=7.6000
13421
PRIMER_PAIR_COMPL_ANY=6.00
13422
PRIMER_PAIR_COMPL_END=0.00
13423
PRIMER_PRODUCT_SIZE=172
13424
PRIMER_PAIR_PENALTY_1=1.0010
13425
PRIMER_LEFT_1_PENALTY=0.015502
13426
PRIMER_RIGHT_1_PENALTY=0.985544
13427
PRIMER_LEFT_1_SEQUENCE=ATATTTTCAGAATGCCCCCC
13428
PRIMER_RIGHT_1_SEQUENCE=TCCAGAAGCTCTGGAAACCT
13429
PRIMER_LEFT_1=122,20
13430
PRIMER_RIGHT_1=294,20
13431
PRIMER_LEFT_1_TM=59.984
13432
PRIMER_RIGHT_1_TM=59.014
13433
PRIMER_LEFT_1_GC_PERCENT=45.000
13434
PRIMER_RIGHT_1_GC_PERCENT=50.000
13435
PRIMER_LEFT_1_SELF_ANY=5.00
13436
PRIMER_RIGHT_1_SELF_ANY=10.00
13437
PRIMER_LEFT_1_SELF_END=0.00
13438
PRIMER_RIGHT_1_SELF_END=2.00
13439
PRIMER_LEFT_1_END_STABILITY=12.4000
13440
PRIMER_RIGHT_1_END_STABILITY=7.9000
13441
PRIMER_PAIR_1_COMPL_ANY=6.00
13442
PRIMER_PAIR_1_COMPL_END=0.00
13443
PRIMER_PRODUCT_SIZE_1=173
13444
PRIMER_PAIR_PENALTY_2=1.0174
13445
PRIMER_LEFT_2_PENALTY=0.015502
13446
PRIMER_RIGHT_2_PENALTY=1.001941
13447
PRIMER_LEFT_2_SEQUENCE=ATATTTTCAGAATGCCCCCC
13448
PRIMER_RIGHT_2_SEQUENCE=CCAGAAGCTCTGGAAACCTCT
13449
PRIMER_LEFT_2=122,20
13450
PRIMER_RIGHT_2=293,21
13451
PRIMER_LEFT_2_TM=59.984
13452
PRIMER_RIGHT_2_TM=60.002
13453
PRIMER_LEFT_2_GC_PERCENT=45.000
13454
PRIMER_RIGHT_2_GC_PERCENT=52.381
13455
PRIMER_LEFT_2_SELF_ANY=5.00
13456
PRIMER_RIGHT_2_SELF_ANY=8.00
13457
PRIMER_LEFT_2_SELF_END=0.00
13458
PRIMER_RIGHT_2_SELF_END=2.00
13459
PRIMER_LEFT_2_END_STABILITY=12.4000
13460
PRIMER_RIGHT_2_END_STABILITY=7.9000
13461
PRIMER_PAIR_2_COMPL_ANY=6.00
13462
PRIMER_PAIR_2_COMPL_END=0.00
13463
PRIMER_PRODUCT_SIZE_2=172
13464
PRIMER_PAIR_PENALTY_3=1.1451
13465
PRIMER_LEFT_3_PENALTY=0.015502
13466
PRIMER_RIGHT_3_PENALTY=1.129584
13467
PRIMER_LEFT_3_SEQUENCE=ATATTTTCAGAATGCCCCCC
13468
PRIMER_RIGHT_3_SEQUENCE=GTCCAGAAGCTCTGGAAACCT
13469
PRIMER_LEFT_3=122,20
13470
PRIMER_RIGHT_3=295,21
13471
PRIMER_LEFT_3_TM=59.984
13472
PRIMER_RIGHT_3_TM=59.870
13473
PRIMER_LEFT_3_GC_PERCENT=45.000
13474
PRIMER_RIGHT_3_GC_PERCENT=52.381
13475
PRIMER_LEFT_3_SELF_ANY=5.00
13476
PRIMER_RIGHT_3_SELF_ANY=10.00
13477
PRIMER_LEFT_3_SELF_END=0.00
13478
PRIMER_RIGHT_3_SELF_END=2.00
13479
PRIMER_LEFT_3_END_STABILITY=12.4000
13480
PRIMER_RIGHT_3_END_STABILITY=7.9000
13481
PRIMER_PAIR_3_COMPL_ANY=6.00
13482
PRIMER_PAIR_3_COMPL_END=0.00
13483
PRIMER_PRODUCT_SIZE_3=174
13484
PRIMER_PAIR_PENALTY_4=1.1472
13485
PRIMER_LEFT_4_PENALTY=0.015502
13486
PRIMER_RIGHT_4_PENALTY=1.131705
13487
PRIMER_LEFT_4_SEQUENCE=ATATTTTCAGAATGCCCCCC
13488
PRIMER_RIGHT_4_SEQUENCE=GTCCAGAAGCTCTGGAAACC
13489
PRIMER_LEFT_4=122,20
13490
PRIMER_RIGHT_4=295,20
13491
PRIMER_LEFT_4_TM=59.984
13492
PRIMER_RIGHT_4_TM=58.868
13493
PRIMER_LEFT_4_GC_PERCENT=45.000
13494
PRIMER_RIGHT_4_GC_PERCENT=55.000
13495
PRIMER_LEFT_4_SELF_ANY=5.00
13496
PRIMER_RIGHT_4_SELF_ANY=10.00
13497
PRIMER_LEFT_4_SELF_END=0.00
13498
PRIMER_RIGHT_4_SELF_END=2.00
13499
PRIMER_LEFT_4_END_STABILITY=12.4000
13500
PRIMER_RIGHT_4_END_STABILITY=8.2000
13501
PRIMER_PAIR_4_COMPL_ANY=6.00
13502
PRIMER_PAIR_4_COMPL_END=0.00
13503
PRIMER_PRODUCT_SIZE_4=174
13504
=
13505
MARKER_NAME=24.RHAP131FH9.seq
13506
SEQUENCE=NNNNNNNNNNNNNNNCTCATTCTCCATCAAATGCCACGAACTTCAATGGCTAACCCTTGGGTTCCGTGTCTATGAGGAAGTATACACAATGCCAATGAGCCAGAGATATTCTGTAACCTTCTATTTCACAGTTCCTGTGATCAGCTCCTTAAAGTGGCAGAGGACATAGGAGTTGGCTTTCTCTAGTCATGTACAGTCTTATAAAAATCATATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGCAAGAGGAAAAATAGATTTAGTAAAAGCAAAAAAAATTGGTAATGGAGAAACAAAAATAATATTGAATCAAGACCTGAGCATTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
13507
INCLUDED_REGION=15,337
13508
TARGET=214,50,GT
13509
PRIMER_COMMENT=CL is GCTCCTTAAAGTGGCAGAGG
13510
PRIMER_COMMENT=CR is TCTCCATTACCAATTTTTTTTGC
13511
PRIMER_PAIR_PENALTY=4.5158
13512
PRIMER_LEFT_PENALTY=0.923358
13513
PRIMER_RIGHT_PENALTY=3.592434
13514
PRIMER_LEFT_SEQUENCE=GCTCCTTAAAGTGGCAGAGG
13515
PRIMER_RIGHT_SEQUENCE=TCTCCATTACCAATTTTTTTTGC
13516
PRIMER_LEFT=143,20
13517
PRIMER_RIGHT=315,23
13518
PRIMER_LEFT_TM=59.077
13519
PRIMER_RIGHT_TM=59.408
13520
PRIMER_LEFT_GC_PERCENT=55.000
13521
PRIMER_RIGHT_GC_PERCENT=30.435
13522
PRIMER_LEFT_SELF_ANY=5.00
13523
PRIMER_RIGHT_SELF_ANY=4.00
13524
PRIMER_LEFT_SELF_END=0.00
13525
PRIMER_RIGHT_SELF_END=2.00
13526
PRIMER_LEFT_END_STABILITY=7.9000
13527
PRIMER_RIGHT_END_STABILITY=8.8000
13528
PRIMER_PAIR_COMPL_ANY=5.00
13529
PRIMER_PAIR_COMPL_END=2.00
13530
PRIMER_PRODUCT_SIZE=173
13531
PRIMER_PAIR_PENALTY_1=4.6184
13532
PRIMER_LEFT_1_PENALTY=1.026007
13533
PRIMER_RIGHT_1_PENALTY=3.592434
13534
PRIMER_LEFT_1_SEQUENCE=AGCTCCTTAAAGTGGCAGAGG
13535
PRIMER_RIGHT_1_SEQUENCE=TCTCCATTACCAATTTTTTTTGC
13536
PRIMER_LEFT_1=142,21
13537
PRIMER_RIGHT_1=315,23
13538
PRIMER_LEFT_1_TM=60.026
13539
PRIMER_RIGHT_1_TM=59.408
13540
PRIMER_LEFT_1_GC_PERCENT=52.381
13541
PRIMER_RIGHT_1_GC_PERCENT=30.435
13542
PRIMER_LEFT_1_SELF_ANY=5.00
13543
PRIMER_RIGHT_1_SELF_ANY=4.00
13544
PRIMER_LEFT_1_SELF_END=0.00
13545
PRIMER_RIGHT_1_SELF_END=2.00
13546
PRIMER_LEFT_1_END_STABILITY=7.9000
13547
PRIMER_RIGHT_1_END_STABILITY=8.8000
13548
PRIMER_PAIR_1_COMPL_ANY=5.00
13549
PRIMER_PAIR_1_COMPL_END=2.00
13550
PRIMER_PRODUCT_SIZE_1=174
13551
PRIMER_PAIR_PENALTY_2=4.7316
13552
PRIMER_LEFT_2_PENALTY=1.139192
13553
PRIMER_RIGHT_2_PENALTY=3.592434
13554
PRIMER_LEFT_2_SEQUENCE=TCCTTAAAGTGGCAGAGGACA
13555
PRIMER_RIGHT_2_SEQUENCE=TCTCCATTACCAATTTTTTTTGC
13556
PRIMER_LEFT_2=145,21
13557
PRIMER_RIGHT_2=315,23
13558
PRIMER_LEFT_2_TM=59.861
13559
PRIMER_RIGHT_2_TM=59.408
13560
PRIMER_LEFT_2_GC_PERCENT=47.619
13561
PRIMER_RIGHT_2_GC_PERCENT=30.435
13562
PRIMER_LEFT_2_SELF_ANY=5.00
13563
PRIMER_RIGHT_2_SELF_ANY=4.00
13564
PRIMER_LEFT_2_SELF_END=0.00
13565
PRIMER_RIGHT_2_SELF_END=2.00
13566
PRIMER_LEFT_2_END_STABILITY=7.9000
13567
PRIMER_RIGHT_2_END_STABILITY=8.8000
13568
PRIMER_PAIR_2_COMPL_ANY=6.00
13569
PRIMER_PAIR_2_COMPL_END=2.00
13570
PRIMER_PRODUCT_SIZE_2=171
13571
PRIMER_PAIR_PENALTY_3=4.8540
13572
PRIMER_LEFT_3_PENALTY=1.261614
13573
PRIMER_RIGHT_3_PENALTY=3.592434
13574
PRIMER_LEFT_3_SEQUENCE=TGGCAGAGGACATAGGAGTTG
13575
PRIMER_RIGHT_3_SEQUENCE=TCTCCATTACCAATTTTTTTTGC
13576
PRIMER_LEFT_3=154,21
13577
PRIMER_RIGHT_3=315,23
13578
PRIMER_LEFT_3_TM=60.262
13579
PRIMER_RIGHT_3_TM=59.408
13580
PRIMER_LEFT_3_GC_PERCENT=52.381
13581
PRIMER_RIGHT_3_GC_PERCENT=30.435
13582
PRIMER_LEFT_3_SELF_ANY=3.00
13583
PRIMER_RIGHT_3_SELF_ANY=4.00
13584
PRIMER_LEFT_3_SELF_END=0.00
13585
PRIMER_RIGHT_3_SELF_END=2.00
13586
PRIMER_LEFT_3_END_STABILITY=6.7000
13587
PRIMER_RIGHT_3_END_STABILITY=8.8000
13588
PRIMER_PAIR_3_COMPL_ANY=4.00
13589
PRIMER_PAIR_3_COMPL_END=3.00
13590
PRIMER_PRODUCT_SIZE_3=162
13591
PRIMER_PAIR_PENALTY_4=4.9347
13592
PRIMER_LEFT_4_PENALTY=0.923358
13593
PRIMER_RIGHT_4_PENALTY=4.011367
13594
PRIMER_LEFT_4_SEQUENCE=GCTCCTTAAAGTGGCAGAGG
13595
PRIMER_RIGHT_4_SEQUENCE=TCCATTACCAATTTTTTTTGCTT
13596
PRIMER_LEFT_4=143,20
13597
PRIMER_RIGHT_4=313,23
13598
PRIMER_LEFT_4_TM=59.077
13599
PRIMER_RIGHT_4_TM=58.989
13600
PRIMER_LEFT_4_GC_PERCENT=55.000
13601
PRIMER_RIGHT_4_GC_PERCENT=26.087
13602
PRIMER_LEFT_4_SELF_ANY=5.00
13603
PRIMER_RIGHT_4_SELF_ANY=4.00
13604
PRIMER_LEFT_4_SELF_END=0.00
13605
PRIMER_RIGHT_4_SELF_END=0.00
13606
PRIMER_LEFT_4_END_STABILITY=7.9000
13607
PRIMER_RIGHT_4_END_STABILITY=8.5000
13608
PRIMER_PAIR_4_COMPL_ANY=5.00
13609
PRIMER_PAIR_4_COMPL_END=2.00
13610
PRIMER_PRODUCT_SIZE_4=171
13611
=
13612
MARKER_NAME=25.RHAP131FA10.seq
13613
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNCCCAGGAAGATATTTTATACATAAAATATTGAATATGTTTTACCACAATGGGGCATAGCACAATGTAAAGCATTTCACTATCATAATAGAGAGTTTTTGATTCTAGAAATGTGCGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCACAGAGCCCACAGGGAGAGNNNNNNNNNNNNNNAGAGAGAAATACAAAATGTAATTATCTTTAATTCTAAGAACCCTGCTATGATGGTTAGTTTTTATTGCCAACTTGGATGGGTTTAGAACCAGCCTGGCAACAAAACTCAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
13614
INCLUDED_REGION=27,304
13615
TARGET=143,42,AC
13616
TARGET=206,14,AC
13617
PRIMER_COMMENT=CL is TGGGGCATAGCACAATGTAA
13618
PRIMER_COMMENT=CR is CTCTCCCTGTGGGCTCTGT
13619
PRIMER_PAIR_PENALTY=1.4511
13620
PRIMER_LEFT_PENALTY=0.046801
13621
PRIMER_RIGHT_PENALTY=1.404288
13622
PRIMER_LEFT_SEQUENCE=TGGGGCATAGCACAATGTAA
13623
PRIMER_RIGHT_SEQUENCE=CTCTCCCTGTGGGCTCTGT
13624
PRIMER_LEFT=75,20
13625
PRIMER_RIGHT=205,19
13626
PRIMER_LEFT_TM=59.953
13627
PRIMER_RIGHT_TM=60.404
13628
PRIMER_LEFT_GC_PERCENT=45.000
13629
PRIMER_RIGHT_GC_PERCENT=63.158
13630
PRIMER_LEFT_SELF_ANY=5.00
13631
PRIMER_RIGHT_SELF_ANY=3.00
13632
PRIMER_LEFT_SELF_END=1.00
13633
PRIMER_RIGHT_SELF_END=0.00
13634
PRIMER_LEFT_END_STABILITY=6.0000
13635
PRIMER_RIGHT_END_STABILITY=6.4000
13636
PRIMER_PAIR_COMPL_ANY=5.00
13637
PRIMER_PAIR_COMPL_END=2.00
13638
PRIMER_PRODUCT_SIZE=131
13639
PRIMER_PAIR_PENALTY_1=1.4717
13640
PRIMER_LEFT_1_PENALTY=1.404288
13641
PRIMER_RIGHT_1_PENALTY=0.067436
13642
PRIMER_LEFT_1_SEQUENCE=ACAGAGCCCACAGGGAGAG
13643
PRIMER_RIGHT_1_SEQUENCE=GCTGGTTCTAAACCCATCCA
13644
PRIMER_LEFT_1=187,19
13645
PRIMER_RIGHT_1=312,20
13646
PRIMER_LEFT_1_TM=60.404
13647
PRIMER_RIGHT_1_TM=59.933
13648
PRIMER_LEFT_1_GC_PERCENT=63.158
13649
PRIMER_RIGHT_1_GC_PERCENT=50.000
13650
PRIMER_LEFT_1_SELF_ANY=3.00
13651
PRIMER_RIGHT_1_SELF_ANY=6.00
13652
PRIMER_LEFT_1_SELF_END=0.00
13653
PRIMER_RIGHT_1_SELF_END=0.00
13654
PRIMER_LEFT_1_END_STABILITY=6.4000
13655
PRIMER_RIGHT_1_END_STABILITY=8.1000
13656
PRIMER_PAIR_1_COMPL_ANY=4.00
13657
PRIMER_PAIR_1_COMPL_END=1.00
13658
PRIMER_PRODUCT_SIZE_1=126
13659
PRIMER_PAIR_PENALTY_2=1.6257
13660
PRIMER_LEFT_2_PENALTY=0.221368
13661
PRIMER_RIGHT_2_PENALTY=1.404288
13662
PRIMER_LEFT_2_SEQUENCE=ACAATGGGGCATAGCACAAT
13663
PRIMER_RIGHT_2_SEQUENCE=CTCTCCCTGTGGGCTCTGT
13664
PRIMER_LEFT_2=71,20
13665
PRIMER_RIGHT_2=205,19
13666
PRIMER_LEFT_2_TM=60.221
13667
PRIMER_RIGHT_2_TM=60.404
13668
PRIMER_LEFT_2_GC_PERCENT=45.000
13669
PRIMER_RIGHT_2_GC_PERCENT=63.158
13670
PRIMER_LEFT_2_SELF_ANY=4.00
13671
PRIMER_RIGHT_2_SELF_ANY=3.00
13672
PRIMER_LEFT_2_SELF_END=4.00
13673
PRIMER_RIGHT_2_SELF_END=0.00
13674
PRIMER_LEFT_2_END_STABILITY=6.6000
13675
PRIMER_RIGHT_2_END_STABILITY=6.4000
13676
PRIMER_PAIR_2_COMPL_ANY=5.00
13677
PRIMER_PAIR_2_COMPL_END=2.00
13678
PRIMER_PRODUCT_SIZE_2=135
13679
PRIMER_PAIR_PENALTY_3=1.6257
13680
PRIMER_LEFT_3_PENALTY=0.221368
13681
PRIMER_RIGHT_3_PENALTY=1.404288
13682
PRIMER_LEFT_3_SEQUENCE=AATGGGGCATAGCACAATGT
13683
PRIMER_RIGHT_3_SEQUENCE=CTCTCCCTGTGGGCTCTGT
13684
PRIMER_LEFT_3=73,20
13685
PRIMER_RIGHT_3=205,19
13686
PRIMER_LEFT_3_TM=60.221
13687
PRIMER_RIGHT_3_TM=60.404
13688
PRIMER_LEFT_3_GC_PERCENT=45.000
13689
PRIMER_RIGHT_3_GC_PERCENT=63.158
13690
PRIMER_LEFT_3_SELF_ANY=5.00
13691
PRIMER_RIGHT_3_SELF_ANY=3.00
13692
PRIMER_LEFT_3_SELF_END=5.00
13693
PRIMER_RIGHT_3_SELF_END=0.00
13694
PRIMER_LEFT_3_END_STABILITY=6.6000
13695
PRIMER_RIGHT_3_END_STABILITY=6.4000
13696
PRIMER_PAIR_3_COMPL_ANY=5.00
13697
PRIMER_PAIR_3_COMPL_END=2.00
13698
PRIMER_PRODUCT_SIZE_3=133
13699
PRIMER_PAIR_PENALTY_4=1.6532
13700
PRIMER_LEFT_4_PENALTY=1.404288
13701
PRIMER_RIGHT_4_PENALTY=0.248900
13702
PRIMER_LEFT_4_SEQUENCE=ACAGAGCCCACAGGGAGAG
13703
PRIMER_RIGHT_4_SEQUENCE=GTTGCCAGGCTGGTTCTAAA
13704
PRIMER_LEFT_4=187,19
13705
PRIMER_RIGHT_4=320,20
13706
PRIMER_LEFT_4_TM=60.404
13707
PRIMER_RIGHT_4_TM=60.249
13708
PRIMER_LEFT_4_GC_PERCENT=63.158
13709
PRIMER_RIGHT_4_GC_PERCENT=50.000
13710
PRIMER_LEFT_4_SELF_ANY=3.00
13711
PRIMER_RIGHT_4_SELF_ANY=7.00
13712
PRIMER_LEFT_4_SELF_END=0.00
13713
PRIMER_RIGHT_4_SELF_END=2.00
13714
PRIMER_LEFT_4_END_STABILITY=6.4000
13715
PRIMER_RIGHT_4_END_STABILITY=6.3000
13716
PRIMER_PAIR_4_COMPL_ANY=4.00
13717
PRIMER_PAIR_4_COMPL_END=0.00
13718
PRIMER_PRODUCT_SIZE_4=134
13719
=
13720
MARKER_NAME=26.RHAP131FA11.seq
13721
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAGCCCTACTACAACAAATCAATCTAAAAATAGACCTTTTATTAGCTCACAATTTTAAGGATTTTCTTTTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
13722
INCLUDED_REGION=15,130
13723
TARGET=15,57,AC
13724
PRIMER_COMMENT=CL is
13725
PRIMER_COMMENT=CR is
13726
=
13727
MARKER_NAME=26.RHAP131FA5.seq
13728
SEQUENCE=CCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAACTCCTCATTGTTGTTTTGATTTCAGGTTTATGCTAACTGAATTTTGTGTTACCTTGACTGGGTCATGAGACCATGCTATTCCTTTGAACCTTTTTCTGACTTTTGTGAGGACACTTTCTGTTGAGCTCAATATAGACTGATGAAAGTAGATTTTCCTCCCTGACAGAAGCCCATCTAACCAGCCTGAGCAAAATGATTAGTCTCCTTTCAGGTGGAAGCCAACTCCAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
13729
INCLUDED_REGION=0,277
13730
TARGET=3,42,GT
13731
PRIMER_COMMENT=CL is
13732
PRIMER_COMMENT=CR is
13733
=
13734
MARKER_NAME=27.RHAP131FA12.seq
13735
SEQUENCE=NNNNNNNNNNNNNNCCGACAAAGATGACATGGTTTCTANTGGAGGCAGGGCTGAGATGAGGAGTCACATTCTCTATTGGCAGGCATTTCCCCCTGTCTTGTTATTTTCATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGNNNNNNNNNNNNNNTGTNNCNNNNNGNGNNNNNCNCNACACAACGNNTGTGCGTGAAAANTGAAGTTTTTTGTGTGTCAGTGAGACAGTGTCTTCNTGGGTGCCCGAGGCAANCNAGGGNATCCACCCGAGCGTTAGCCTCCAGANATTATTAACAANGGCCAAAACATCATNCCTACCTAAGTGAGAAAAAGCAATTTNGANAAAAANCGNGCTCCNAATCCAAAACNTGGGCATGGGCTAGATCCNNAGGTGAAAATNGTAGGCGGTCNAGATTCCNAACAACNAANNANCCGGAAACATGNTGTGTGATAACCANAGGGTGACNNNNGAGTNANNNACACAAAATAANTGCGGNGCGCACAAAGNGCGNNNTCCAGNGGGGAAACCTGNGNGNGCCAACTTNNATAAANNNGNNGGGAANNANCGGGNGNGTTGCGAGGTTGNGNAANNNNNCCNCGGNAGNNTNGTANNNNCNCGNNNANGNGGGGGGAGNGNGNTTGCNNNNNGGGNNNCCTGNNNANNNTTNNNANAGTGNAANGGGGANNNCCAANNNNGNAA
13736
INCLUDED_REGION=14,687
13737
TARGET=141,14,TC
13738
TARGET=111,28,TGTC
13739
PRIMER_COMMENT=CL is CTCTATTGGCAGGCATTTCC
13740
PRIMER_COMMENT=CR is AGACACTGTCTCACTGACACACAA
13741
PRIMER_PAIR_PENALTY=4.4206
13742
PRIMER_LEFT_PENALTY=0.332028
13743
PRIMER_RIGHT_PENALTY=4.088575
13744
PRIMER_LEFT_SEQUENCE=CTCTATTGGCAGGCATTTCC
13745
PRIMER_RIGHT_SEQUENCE=AGACACTGTCTCACTGACACACAA
13746
PRIMER_LEFT=70,20
13747
PRIMER_RIGHT=233,24
13748
PRIMER_LEFT_TM=59.668
13749
PRIMER_RIGHT_TM=59.911
13750
PRIMER_LEFT_GC_PERCENT=50.000
13751
PRIMER_RIGHT_GC_PERCENT=45.833
13752
PRIMER_LEFT_SELF_ANY=3.00
13753
PRIMER_RIGHT_SELF_ANY=9.00
13754
PRIMER_LEFT_SELF_END=3.00
13755
PRIMER_RIGHT_SELF_END=3.00
13756
PRIMER_LEFT_END_STABILITY=8.5000
13757
PRIMER_RIGHT_END_STABILITY=7.0000
13758
PRIMER_PAIR_COMPL_ANY=4.00
13759
PRIMER_PAIR_COMPL_END=0.00
13760
PRIMER_PRODUCT_SIZE=164
13761
PRIMER_PAIR_PENALTY_1=4.4206
13762
PRIMER_LEFT_1_PENALTY=0.332028
13763
PRIMER_RIGHT_1_PENALTY=4.088575
13764
PRIMER_LEFT_1_SEQUENCE=CTCTATTGGCAGGCATTTCC
13765
PRIMER_RIGHT_1_SEQUENCE=AAGACACTGTCTCACTGACACACA
13766
PRIMER_LEFT_1=70,20
13767
PRIMER_RIGHT_1=234,24
13768
PRIMER_LEFT_1_TM=59.668
13769
PRIMER_RIGHT_1_TM=59.911
13770
PRIMER_LEFT_1_GC_PERCENT=50.000
13771
PRIMER_RIGHT_1_GC_PERCENT=45.833
13772
PRIMER_LEFT_1_SELF_ANY=3.00
13773
PRIMER_RIGHT_1_SELF_ANY=9.00
13774
PRIMER_LEFT_1_SELF_END=3.00
13775
PRIMER_RIGHT_1_SELF_END=5.00
13776
PRIMER_LEFT_1_END_STABILITY=8.5000
13777
PRIMER_RIGHT_1_END_STABILITY=6.4000
13778
PRIMER_PAIR_1_COMPL_ANY=4.00
13779
PRIMER_PAIR_1_COMPL_END=0.00
13780
PRIMER_PRODUCT_SIZE_1=165
13781
PRIMER_PAIR_PENALTY_2=4.4381
13782
PRIMER_LEFT_2_PENALTY=0.332028
13783
PRIMER_RIGHT_2_PENALTY=4.106055
13784
PRIMER_LEFT_2_SEQUENCE=CTCTATTGGCAGGCATTTCC
13785
PRIMER_RIGHT_2_SEQUENCE=CACTGTCTCACTGACACACAAAA
13786
PRIMER_LEFT_2=70,20
13787
PRIMER_RIGHT_2=230,23
13788
PRIMER_LEFT_2_TM=59.668
13789
PRIMER_RIGHT_2_TM=58.894
13790
PRIMER_LEFT_2_GC_PERCENT=50.000
13791
PRIMER_RIGHT_2_GC_PERCENT=43.478
13792
PRIMER_LEFT_2_SELF_ANY=3.00
13793
PRIMER_RIGHT_2_SELF_ANY=9.00
13794
PRIMER_LEFT_2_SELF_END=3.00
13795
PRIMER_RIGHT_2_SELF_END=0.00
13796
PRIMER_LEFT_2_END_STABILITY=8.5000
13797
PRIMER_RIGHT_2_END_STABILITY=7.6000
13798
PRIMER_PAIR_2_COMPL_ANY=4.00
13799
PRIMER_PAIR_2_COMPL_END=1.00
13800
PRIMER_PRODUCT_SIZE_2=161
13801
PRIMER_PAIR_PENALTY_3=4.4817
13802
PRIMER_LEFT_3_PENALTY=0.332028
13803
PRIMER_RIGHT_3_PENALTY=4.149625
13804
PRIMER_LEFT_3_SEQUENCE=CTCTATTGGCAGGCATTTCC
13805
PRIMER_RIGHT_3_SEQUENCE=GACACTGTCTCACTGACACACAA
13806
PRIMER_LEFT_3=70,20
13807
PRIMER_RIGHT_3=232,23
13808
PRIMER_LEFT_3_TM=59.668
13809
PRIMER_RIGHT_3_TM=58.850
13810
PRIMER_LEFT_3_GC_PERCENT=50.000
13811
PRIMER_RIGHT_3_GC_PERCENT=47.826
13812
PRIMER_LEFT_3_SELF_ANY=3.00
13813
PRIMER_RIGHT_3_SELF_ANY=9.00
13814
PRIMER_LEFT_3_SELF_END=3.00
13815
PRIMER_RIGHT_3_SELF_END=3.00
13816
PRIMER_LEFT_3_END_STABILITY=8.5000
13817
PRIMER_RIGHT_3_END_STABILITY=7.0000
13818
PRIMER_PAIR_3_COMPL_ANY=4.00
13819
PRIMER_PAIR_3_COMPL_END=0.00
13820
PRIMER_PRODUCT_SIZE_3=163
13821
PRIMER_PAIR_PENALTY_4=4.5564
13822
PRIMER_LEFT_4_PENALTY=0.332028
13823
PRIMER_RIGHT_4_PENALTY=4.224341
13824
PRIMER_LEFT_4_SEQUENCE=CTCTATTGGCAGGCATTTCC
13825
PRIMER_RIGHT_4_SEQUENCE=ACACTGTCTCACTGACACACAAAA
13826
PRIMER_LEFT_4=70,20
13827
PRIMER_RIGHT_4=231,24
13828
PRIMER_LEFT_4_TM=59.668
13829
PRIMER_RIGHT_4_TM=59.776
13830
PRIMER_LEFT_4_GC_PERCENT=50.000
13831
PRIMER_RIGHT_4_GC_PERCENT=41.667
13832
PRIMER_LEFT_4_SELF_ANY=3.00
13833
PRIMER_RIGHT_4_SELF_ANY=9.00
13834
PRIMER_LEFT_4_SELF_END=3.00
13835
PRIMER_RIGHT_4_SELF_END=0.00
13836
PRIMER_LEFT_4_END_STABILITY=8.5000
13837
PRIMER_RIGHT_4_END_STABILITY=7.6000
13838
PRIMER_PAIR_4_COMPL_ANY=4.00
13839
PRIMER_PAIR_4_COMPL_END=1.00
13840
PRIMER_PRODUCT_SIZE_4=162
13841
=
13842
MARKER_NAME=29.RHAP131FB5.seq
13843
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCCTTGGTGCATGCAGCCACATTTCCTCAAGGAGCATAGGGCTTATACAGTTCTTTCAGCACAAGTTTTGGGTCTTTTGTTCCACATTCACATGTCAGACTTGCTAGCCGGTTTTTTGTATATGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTATATGTATTTGTGTTATATGTGTGTGTGTGTTTGCAAGTGCGTGGTTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATTTGTATTCGTGTTATTTGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTGTTGTGTGTGTGCTATTTCTATNNGTTTTTATNTGTANNNNNNNNNNNNNNNNATNTGCGTGTGTGTGTGCNNNNNNNNNNNNNNNNNNNNNGTGTNNTCCNCNCCNGTATTTCACACNNAANACGTNNANNGNGAGAATTCCTNAAAATCNGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
13844
INCLUDED_REGION=23,477
13845
TARGET=148,44,TG
13846
TARGET=243,35,GT
13847
TARGET=301,40,TG
13848
TARGET=382,16,TG
13849
TARGET=416,18,TG
13850
PRIMER_COMMENT=CL is CATGTCAGACTTGCTAGCCG
13851
PRIMER_COMMENT=CR is ACGCACTTGCAAACACACAC
13852
PRIMER_PAIR_PENALTY=1.2189
13853
PRIMER_LEFT_PENALTY=0.384829
13854
PRIMER_RIGHT_PENALTY=0.834091
13855
PRIMER_LEFT_SEQUENCE=CATGTCAGACTTGCTAGCCG
13856
PRIMER_RIGHT_SEQUENCE=ACGCACTTGCAAACACACAC
13857
PRIMER_LEFT=112,20
13858
PRIMER_RIGHT=236,20
13859
PRIMER_LEFT_TM=59.615
13860
PRIMER_RIGHT_TM=60.834
13861
PRIMER_LEFT_GC_PERCENT=55.000
13862
PRIMER_RIGHT_GC_PERCENT=50.000
13863
PRIMER_LEFT_SELF_ANY=7.00
13864
PRIMER_RIGHT_SELF_ANY=6.00
13865
PRIMER_LEFT_SELF_END=2.00
13866
PRIMER_RIGHT_SELF_END=0.00
13867
PRIMER_LEFT_END_STABILITY=11.4000
13868
PRIMER_RIGHT_END_STABILITY=6.4000
13869
PRIMER_PAIR_COMPL_ANY=5.00
13870
PRIMER_PAIR_COMPL_END=2.00
13871
PRIMER_PRODUCT_SIZE=125
13872
PRIMER_PAIR_PENALTY_1=1.4561
13873
PRIMER_LEFT_1_PENALTY=0.384829
13874
PRIMER_RIGHT_1_PENALTY=1.071294
13875
PRIMER_LEFT_1_SEQUENCE=CATGTCAGACTTGCTAGCCG
13876
PRIMER_RIGHT_1_SEQUENCE=CACGCACTTGCAAACACAC
13877
PRIMER_LEFT_1=112,20
13878
PRIMER_RIGHT_1=237,19
13879
PRIMER_LEFT_1_TM=59.615
13880
PRIMER_RIGHT_1_TM=59.929
13881
PRIMER_LEFT_1_GC_PERCENT=55.000
13882
PRIMER_RIGHT_1_GC_PERCENT=52.632
13883
PRIMER_LEFT_1_SELF_ANY=7.00
13884
PRIMER_RIGHT_1_SELF_ANY=6.00
13885
PRIMER_LEFT_1_SELF_END=2.00
13886
PRIMER_RIGHT_1_SELF_END=0.00
13887
PRIMER_LEFT_1_END_STABILITY=11.4000
13888
PRIMER_RIGHT_1_END_STABILITY=6.4000
13889
PRIMER_PAIR_1_COMPL_ANY=5.00
13890
PRIMER_PAIR_1_COMPL_END=2.00
13891
PRIMER_PRODUCT_SIZE_1=126
13892
PRIMER_PAIR_PENALTY_2=1.4561
13893
PRIMER_LEFT_2_PENALTY=0.384829
13894
PRIMER_RIGHT_2_PENALTY=1.071294
13895
PRIMER_LEFT_2_SEQUENCE=CATGTCAGACTTGCTAGCCG
13896
PRIMER_RIGHT_2_SEQUENCE=ACGCACTTGCAAACACACA
13897
PRIMER_LEFT_2=112,20
13898
PRIMER_RIGHT_2=236,19
13899
PRIMER_LEFT_2_TM=59.615
13900
PRIMER_RIGHT_2_TM=59.929
13901
PRIMER_LEFT_2_GC_PERCENT=55.000
13902
PRIMER_RIGHT_2_GC_PERCENT=47.368
13903
PRIMER_LEFT_2_SELF_ANY=7.00
13904
PRIMER_RIGHT_2_SELF_ANY=6.00
13905
PRIMER_LEFT_2_SELF_END=2.00
13906
PRIMER_RIGHT_2_SELF_END=0.00
13907
PRIMER_LEFT_2_END_STABILITY=11.4000
13908
PRIMER_RIGHT_2_END_STABILITY=6.4000
13909
PRIMER_PAIR_2_COMPL_ANY=5.00
13910
PRIMER_PAIR_2_COMPL_END=3.00
13911
PRIMER_PRODUCT_SIZE_2=125
13912
PRIMER_PAIR_PENALTY_3=1.4900
13913
PRIMER_LEFT_3_PENALTY=0.418685
13914
PRIMER_RIGHT_3_PENALTY=1.071294
13915
PRIMER_LEFT_3_SEQUENCE=ATGTCAGACTTGCTAGCCGG
13916
PRIMER_RIGHT_3_SEQUENCE=CACGCACTTGCAAACACAC
13917
PRIMER_LEFT_3=113,20
13918
PRIMER_RIGHT_3=237,19
13919
PRIMER_LEFT_3_TM=60.419
13920
PRIMER_RIGHT_3_TM=59.929
13921
PRIMER_LEFT_3_GC_PERCENT=55.000
13922
PRIMER_RIGHT_3_GC_PERCENT=52.632
13923
PRIMER_LEFT_3_SELF_ANY=6.00
13924
PRIMER_RIGHT_3_SELF_ANY=6.00
13925
PRIMER_LEFT_3_SELF_END=4.00
13926
PRIMER_RIGHT_3_SELF_END=0.00
13927
PRIMER_LEFT_3_END_STABILITY=12.9000
13928
PRIMER_RIGHT_3_END_STABILITY=6.4000
13929
PRIMER_PAIR_3_COMPL_ANY=5.00
13930
PRIMER_PAIR_3_COMPL_END=1.00
13931
PRIMER_PRODUCT_SIZE_3=125
13932
PRIMER_PAIR_PENALTY_4=1.8388
13933
PRIMER_LEFT_4_PENALTY=1.004672
13934
PRIMER_RIGHT_4_PENALTY=0.834091
13935
PRIMER_LEFT_4_SEQUENCE=TTTGGGTCTTTTGTTCCACA
13936
PRIMER_RIGHT_4_SEQUENCE=ACGCACTTGCAAACACACAC
13937
PRIMER_LEFT_4=88,20
13938
PRIMER_RIGHT_4=236,20
13939
PRIMER_LEFT_4_TM=58.995
13940
PRIMER_RIGHT_4_TM=60.834
13941
PRIMER_LEFT_4_GC_PERCENT=40.000
13942
PRIMER_RIGHT_4_GC_PERCENT=50.000
13943
PRIMER_LEFT_4_SELF_ANY=3.00
13944
PRIMER_RIGHT_4_SELF_ANY=6.00
13945
PRIMER_LEFT_4_SELF_END=3.00
13946
PRIMER_RIGHT_4_SELF_END=0.00
13947
PRIMER_LEFT_4_END_STABILITY=8.2000
13948
PRIMER_RIGHT_4_END_STABILITY=6.4000
13949
PRIMER_PAIR_4_COMPL_ANY=5.00
13950
PRIMER_PAIR_4_COMPL_END=2.00
13951
PRIMER_PRODUCT_SIZE_4=149
13952
=
13953
MARKER_NAME=30.RHAP131FB12.seq
13954
SEQUENCE=NNNNNNNNNNNNNNCCGGACAGCTTTCTCCCTAGCTTTTCATTTCTGACAATAGTGCTCTGCCTTGATTCTAGAATGATTTCATGGAGGGAAGTTTGTCCTTTTTAGAGCTATCACAGCTTCACAGTCCTACCCACTTCACTTGTTTCTATGCCAATTCCCTAATACTTGGAGCAGTAATCACACAGCAATACCACAAGGGTGCTTACCCACAGTGCTTTCCTCAGTCAGGAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCATGGTGCTTACCCACAGTGCTTTCCTTAGTCAGGAGCGTGTGTGTGTGTGGGGGGGGGGGGAGGCCAAGATGATAGCCCCTGGGATGGCACAGGTTACTTCCTTGGAGGACCTAACACCAAGACCAATGTGACCATTGCAGCCCACATCTGCACCATCTTTAGAACAACATCCTTTAGGGATACCCTCAAGAAAACACCAGTGATCAGTCTGTGATAGGCAGAGGACAGGTAGGGCNCNTCAAGCCCTTGATCCNCGTGTCNNTGACCACGCCTGGTGATGGGGATCTCNCGTCTTGTTTACCCCATGAGATGGCAGACCCACATGGGGGACGAGCACGNATCNGAAACATGGCAGAGNTGTTCCTGTGTAAANTGGTANCGGCACACTC
13955
INCLUDED_REGION=14,666
13956
TARGET=234,54,GT
13957
PRIMER_COMMENT=CL is GCAATACCACAAGGGTGCTT
13958
PRIMER_COMMENT=CR is GGAAAGCACTGTGGGTAAGC
13959
PRIMER_PAIR_PENALTY=0.2597
13960
PRIMER_LEFT_PENALTY=0.000037
13961
PRIMER_RIGHT_PENALTY=0.259704
13962
PRIMER_LEFT_SEQUENCE=GCAATACCACAAGGGTGCTT
13963
PRIMER_RIGHT_SEQUENCE=GGAAAGCACTGTGGGTAAGC
13964
PRIMER_LEFT=186,20
13965
PRIMER_RIGHT=314,20
13966
PRIMER_LEFT_TM=60.000
13967
PRIMER_RIGHT_TM=59.740
13968
PRIMER_LEFT_GC_PERCENT=50.000
13969
PRIMER_RIGHT_GC_PERCENT=55.000
13970
PRIMER_LEFT_SELF_ANY=3.00
13971
PRIMER_RIGHT_SELF_ANY=5.00
13972
PRIMER_LEFT_SELF_END=2.00
13973
PRIMER_RIGHT_SELF_END=2.00
13974
PRIMER_LEFT_END_STABILITY=8.5000
13975
PRIMER_RIGHT_END_STABILITY=7.5000
13976
PRIMER_PAIR_COMPL_ANY=6.00
13977
PRIMER_PAIR_COMPL_END=5.00
13978
PRIMER_PRODUCT_SIZE=129
13979
PRIMER_PAIR_PENALTY_1=0.5097
13980
PRIMER_LEFT_1_PENALTY=0.259704
13981
PRIMER_RIGHT_1_PENALTY=0.249979
13982
PRIMER_LEFT_1_SEQUENCE=GCTTACCCACAGTGCTTTCC
13983
PRIMER_RIGHT_1_SEQUENCE=ATCCCAGGGGCTATCATCTT
13984
PRIMER_LEFT_1=202,20
13985
PRIMER_RIGHT_1=375,20
13986
PRIMER_LEFT_1_TM=59.740
13987
PRIMER_RIGHT_1_TM=59.750
13988
PRIMER_LEFT_1_GC_PERCENT=55.000
13989
PRIMER_RIGHT_1_GC_PERCENT=50.000
13990
PRIMER_LEFT_1_SELF_ANY=5.00
13991
PRIMER_RIGHT_1_SELF_ANY=5.00
13992
PRIMER_LEFT_1_SELF_END=0.00
13993
PRIMER_RIGHT_1_SELF_END=0.00
13994
PRIMER_LEFT_1_END_STABILITY=8.5000
13995
PRIMER_RIGHT_1_END_STABILITY=6.6000
13996
PRIMER_PAIR_1_COMPL_ANY=3.00
13997
PRIMER_PAIR_1_COMPL_END=1.00
13998
PRIMER_PRODUCT_SIZE_1=174
13999
PRIMER_PAIR_PENALTY_2=0.5480
14000
PRIMER_LEFT_2_PENALTY=0.298018
14001
PRIMER_RIGHT_2_PENALTY=0.249979
14002
PRIMER_LEFT_2_SEQUENCE=CCCACAGTGCTTTCCTCAGT
14003
PRIMER_RIGHT_2_SEQUENCE=ATCCCAGGGGCTATCATCTT
14004
PRIMER_LEFT_2=207,20
14005
PRIMER_RIGHT_2=375,20
14006
PRIMER_LEFT_2_TM=60.298
14007
PRIMER_RIGHT_2_TM=59.750
14008
PRIMER_LEFT_2_GC_PERCENT=55.000
14009
PRIMER_RIGHT_2_GC_PERCENT=50.000
14010
PRIMER_LEFT_2_SELF_ANY=5.00
14011
PRIMER_RIGHT_2_SELF_ANY=5.00
14012
PRIMER_LEFT_2_SELF_END=1.00
14013
PRIMER_RIGHT_2_SELF_END=0.00
14014
PRIMER_LEFT_2_END_STABILITY=6.4000
14015
PRIMER_RIGHT_2_END_STABILITY=6.6000
14016
PRIMER_PAIR_2_COMPL_ANY=3.00
14017
PRIMER_PAIR_2_COMPL_END=0.00
14018
PRIMER_PRODUCT_SIZE_2=169
14019
PRIMER_PAIR_PENALTY_3=0.5480
14020
PRIMER_LEFT_3_PENALTY=0.298018
14021
PRIMER_RIGHT_3_PENALTY=0.249979
14022
PRIMER_LEFT_3_SEQUENCE=ACCCACAGTGCTTTCCTCAG
14023
PRIMER_RIGHT_3_SEQUENCE=ATCCCAGGGGCTATCATCTT
14024
PRIMER_LEFT_3=206,20
14025
PRIMER_RIGHT_3=375,20
14026
PRIMER_LEFT_3_TM=60.298
14027
PRIMER_RIGHT_3_TM=59.750
14028
PRIMER_LEFT_3_GC_PERCENT=55.000
14029
PRIMER_RIGHT_3_GC_PERCENT=50.000
14030
PRIMER_LEFT_3_SELF_ANY=5.00
14031
PRIMER_RIGHT_3_SELF_ANY=5.00
14032
PRIMER_LEFT_3_SELF_END=3.00
14033
PRIMER_RIGHT_3_SELF_END=0.00
14034
PRIMER_LEFT_3_END_STABILITY=6.7000
14035
PRIMER_RIGHT_3_END_STABILITY=6.6000
14036
PRIMER_PAIR_3_COMPL_ANY=3.00
14037
PRIMER_PAIR_3_COMPL_END=1.00
14038
PRIMER_PRODUCT_SIZE_3=170
14039
PRIMER_PAIR_PENALTY_4=0.5577
14040
PRIMER_LEFT_4_PENALTY=0.378958
14041
PRIMER_RIGHT_4_PENALTY=0.178718
14042
PRIMER_LEFT_4_SEQUENCE=AGCAATACCACAAGGGTGCT
14043
PRIMER_RIGHT_4_SEQUENCE=AAGCACTGTGGGTAAGCACC
14044
PRIMER_LEFT_4=185,20
14045
PRIMER_RIGHT_4=311,20
14046
PRIMER_LEFT_4_TM=59.621
14047
PRIMER_RIGHT_4_TM=60.179
14048
PRIMER_LEFT_4_GC_PERCENT=50.000
14049
PRIMER_RIGHT_4_GC_PERCENT=55.000
14050
PRIMER_LEFT_4_SELF_ANY=4.00
14051
PRIMER_RIGHT_4_SELF_ANY=5.00
14052
PRIMER_LEFT_4_SELF_END=2.00
14053
PRIMER_RIGHT_4_SELF_END=2.00
14054
PRIMER_LEFT_4_END_STABILITY=7.9000
14055
PRIMER_RIGHT_4_END_STABILITY=9.4000
14056
PRIMER_PAIR_4_COMPL_ANY=6.00
14057
PRIMER_PAIR_4_COMPL_END=6.00
14058
PRIMER_PRODUCT_SIZE_4=127
14059
=
14060
MARKER_NAME=31.RHAP131FC4.seq
14061
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNCAAATGTGAGCAGATACTCTACTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAATGTCATGATAAGGACCACTTTTGTACACTAACAAAAAACTGGATTTAAGCAATATATTTTAAAAACCTCAGCTATATAATAACAAAATAAAAGGGATAATAGAGCTATATGACATCTTACACACATATGCACACCATAGATGTTGTGTAGAGAAAGATGACACTGAGTTCCTGACCCCCAGCTCCTCCCACTGAGTACTATAATCACAGGTGTGTACCAGCAGTCCAGTTTCATTGCNTTAAGAATTAAAGCCAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
14062
INCLUDED_REGION=24,327
14063
TARGET=48,42,TG
14064
PRIMER_COMMENT=CL is
14065
PRIMER_COMMENT=CR is
14066
=
14067
MARKER_NAME=32.RHAP131FC5.seq
14068
SEQUENCE=NNNNNNNNNNNCCCTGTGTTTCATTCTGAGTGCTACAGAGAAGCATTTTTGACAAGTTTAAGCTCTTACTTAAACTTGAGACCAATGATGAGTGAAAGGCATATCCTATCAAATTGCATACATGATATGCAAAATTAAGTCTACATTTTAACATGCCTCGCCTATTGTGGACATATTCTTTTATGAATTGTGTTTAACTACTTACTAAAGGTAGCTCTTTTCCTTTGTGTGTGCATGCACNNNNNNNNNNNNNNNNNNNNNNNNNNAGNGNGACNNNNNNNNNNNNNNNNNNNNNNNNAAAAGAACAGNTTTTNAAAAGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
14069
INCLUDED_REGION=11,311
14070
TARGET=240,26,GT
14071
TARGET=274,24,GT
14072
PRIMER_COMMENT=CL is
14073
PRIMER_COMMENT=CR is
14074
=
14075
MARKER_NAME=33.RHAP131FC12.seq
14076
SEQUENCE=NNNNNNNNNNNNNTTGTATTAGAACACTGAATAACTCTTATCTTTACTCTGTAATTTAAAAATTACATTCTTCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGCATATATGGAAGTCAGAGGACAATTTGTGGGAAGTTCTCTCTCTATACTGTGTGGGTCCTGAGGAATGAATTTATGTCACCAGGCTTGGTGACCAGCACCTTGACTGGATAATCCACCTGCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
14077
INCLUDED_REGION=13,232
14078
TARGET=75,44,GT
14079
PRIMER_COMMENT=CL is
14080
PRIMER_COMMENT=CR is
14081
=
14082
MARKER_NAME=33.RHAP131FC6.seq
14083
SEQUENCE=NNNNNNNNNNCCGCCTCCAAGGACTCCAAGACTGGATGCACACGCTGAGGCAAGACTGCTGAGGCGACACATGGAGGACACATGATGGTTAGAGGGTATATAATAAGGACTCCACAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGCACTCGGCTTCCTGGTACACTAGTTGTGTCACACTTGCTGGGCTTTGCTGACCTTTGCTTCACTCAGAGAGGCACAGCTGAAGAACGTCTGATGTTCCTGCTGGTCCCTCCTGCTGACTGGAGCCCGGGCTGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
14084
INCLUDED_REGION=10,330
14085
TARGET=118,44,GT
14086
TARGET=163,40,AG
14087
PRIMER_COMMENT=CL is ACATGGAGGACACATGATGG
14088
PRIMER_COMMENT=CR is GTGTACCAGGAAGCCGAGTG
14089
PRIMER_PAIR_PENALTY=1.5269
14090
PRIMER_LEFT_PENALTY=0.815754
14091
PRIMER_RIGHT_PENALTY=0.711184
14092
PRIMER_LEFT_SEQUENCE=ACATGGAGGACACATGATGG
14093
PRIMER_RIGHT_SEQUENCE=GTGTACCAGGAAGCCGAGTG
14094
PRIMER_LEFT=68,20
14095
PRIMER_RIGHT=224,20
14096
PRIMER_LEFT_TM=59.184
14097
PRIMER_RIGHT_TM=60.711
14098
PRIMER_LEFT_GC_PERCENT=50.000
14099
PRIMER_RIGHT_GC_PERCENT=60.000
14100
PRIMER_LEFT_SELF_ANY=5.00
14101
PRIMER_RIGHT_SELF_ANY=4.00
14102
PRIMER_LEFT_SELF_END=3.00
14103
PRIMER_RIGHT_SELF_END=0.00
14104
PRIMER_LEFT_END_STABILITY=8.1000
14105
PRIMER_RIGHT_END_STABILITY=6.4000
14106
PRIMER_PAIR_COMPL_ANY=4.00
14107
PRIMER_PAIR_COMPL_END=0.00
14108
PRIMER_PRODUCT_SIZE=157
14109
PRIMER_PAIR_PENALTY_1=1.5269
14110
PRIMER_LEFT_1_PENALTY=0.815754
14111
PRIMER_RIGHT_1_PENALTY=0.711184
14112
PRIMER_LEFT_1_SEQUENCE=CATGGAGGACACATGATGGT
14113
PRIMER_RIGHT_1_SEQUENCE=GTGTACCAGGAAGCCGAGTG
14114
PRIMER_LEFT_1=69,20
14115
PRIMER_RIGHT_1=224,20
14116
PRIMER_LEFT_1_TM=59.184
14117
PRIMER_RIGHT_1_TM=60.711
14118
PRIMER_LEFT_1_GC_PERCENT=50.000
14119
PRIMER_RIGHT_1_GC_PERCENT=60.000
14120
PRIMER_LEFT_1_SELF_ANY=5.00
14121
PRIMER_RIGHT_1_SELF_ANY=4.00
14122
PRIMER_LEFT_1_SELF_END=5.00
14123
PRIMER_RIGHT_1_SELF_END=0.00
14124
PRIMER_LEFT_1_END_STABILITY=7.8000
14125
PRIMER_RIGHT_1_END_STABILITY=6.4000
14126
PRIMER_PAIR_1_COMPL_ANY=4.00
14127
PRIMER_PAIR_1_COMPL_END=0.00
14128
PRIMER_PRODUCT_SIZE_1=156
14129
PRIMER_PAIR_PENALTY_2=1.7154
14130
PRIMER_LEFT_2_PENALTY=1.004187
14131
PRIMER_RIGHT_2_PENALTY=0.711184
14132
PRIMER_LEFT_2_SEQUENCE=GACTGCTGAGGCGACACAT
14133
PRIMER_RIGHT_2_SEQUENCE=GTGTACCAGGAAGCCGAGTG
14134
PRIMER_LEFT_2=53,19
14135
PRIMER_RIGHT_2=224,20
14136
PRIMER_LEFT_2_TM=60.004
14137
PRIMER_RIGHT_2_TM=60.711
14138
PRIMER_LEFT_2_GC_PERCENT=57.895
14139
PRIMER_RIGHT_2_GC_PERCENT=60.000
14140
PRIMER_LEFT_2_SELF_ANY=4.00
14141
PRIMER_RIGHT_2_SELF_ANY=4.00
14142
PRIMER_LEFT_2_SELF_END=2.00
14143
PRIMER_RIGHT_2_SELF_END=0.00
14144
PRIMER_LEFT_2_END_STABILITY=6.6000
14145
PRIMER_RIGHT_2_END_STABILITY=6.4000
14146
PRIMER_PAIR_2_COMPL_ANY=4.00
14147
PRIMER_PAIR_2_COMPL_END=2.00
14148
PRIMER_PRODUCT_SIZE_2=172
14149
PRIMER_PAIR_PENALTY_3=1.7492
14150
PRIMER_LEFT_3_PENALTY=1.038054
14151
PRIMER_RIGHT_3_PENALTY=0.711184
14152
PRIMER_LEFT_3_SEQUENCE=CGACACATGGAGGACACATG
14153
PRIMER_RIGHT_3_SEQUENCE=GTGTACCAGGAAGCCGAGTG
14154
PRIMER_LEFT_3=64,20
14155
PRIMER_RIGHT_3=224,20
14156
PRIMER_LEFT_3_TM=61.038
14157
PRIMER_RIGHT_3_TM=60.711
14158
PRIMER_LEFT_3_GC_PERCENT=55.000
14159
PRIMER_RIGHT_3_GC_PERCENT=60.000
14160
PRIMER_LEFT_3_SELF_ANY=4.00
14161
PRIMER_RIGHT_3_SELF_ANY=4.00
14162
PRIMER_LEFT_3_SELF_END=4.00
14163
PRIMER_RIGHT_3_SELF_END=0.00
14164
PRIMER_LEFT_3_END_STABILITY=6.6000
14165
PRIMER_RIGHT_3_END_STABILITY=6.4000
14166
PRIMER_PAIR_3_COMPL_ANY=4.00
14167
PRIMER_PAIR_3_COMPL_END=2.00
14168
PRIMER_PRODUCT_SIZE_3=161
14169
PRIMER_PAIR_PENALTY_4=1.7531
14170
PRIMER_LEFT_4_PENALTY=1.041921
14171
PRIMER_RIGHT_4_PENALTY=0.711184
14172
PRIMER_LEFT_4_SEQUENCE=AGACTGCTGAGGCGACACAT
14173
PRIMER_RIGHT_4_SEQUENCE=GTGTACCAGGAAGCCGAGTG
14174
PRIMER_LEFT_4=52,20
14175
PRIMER_RIGHT_4=224,20
14176
PRIMER_LEFT_4_TM=61.042
14177
PRIMER_RIGHT_4_TM=60.711
14178
PRIMER_LEFT_4_GC_PERCENT=55.000
14179
PRIMER_RIGHT_4_GC_PERCENT=60.000
14180
PRIMER_LEFT_4_SELF_ANY=4.00
14181
PRIMER_RIGHT_4_SELF_ANY=4.00
14182
PRIMER_LEFT_4_SELF_END=2.00
14183
PRIMER_RIGHT_4_SELF_END=0.00
14184
PRIMER_LEFT_4_END_STABILITY=6.6000
14185
PRIMER_RIGHT_4_END_STABILITY=6.4000
14186
PRIMER_PAIR_4_COMPL_ANY=4.00
14187
PRIMER_PAIR_4_COMPL_END=2.00
14188
PRIMER_PRODUCT_SIZE_4=173
14189
=
14190
MARKER_NAME=35.RHAP131FD11.seq
14191
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNNNCAGTGGTTAGCAAGCAACACTGAAGAGACTTGTCAGGTTATATTTATATATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGCAACAATGATTAAAGAAGAGGAGGCTGTGAAATTTAAGAGCGAGCAGGGGTGGGTATTGAAGAGATTAGAGGGGGAATGTAATTATGTCTTCATTAACTTTTTCAAATTAAAAAAAAAGAATGTGACTTTAATCATATCTAAATCGGAGCAGACTATCAATCCACATACTCCACTCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
14192
INCLUDED_REGION=25,317
14193
TARGET=77,83,TG
14194
PRIMER_COMMENT=CL is CAACACTGAAGAGACTTGTCAGG
14195
PRIMER_COMMENT=CR is ACCCCTGCTCGCTCTTAAAT
14196
PRIMER_PAIR_PENALTY=4.1803
14197
PRIMER_LEFT_PENALTY=3.951808
14198
PRIMER_RIGHT_PENALTY=0.228464
14199
PRIMER_LEFT_SEQUENCE=CAACACTGAAGAGACTTGTCAGG
14200
PRIMER_RIGHT_SEQUENCE=ACCCCTGCTCGCTCTTAAAT
14201
PRIMER_LEFT=39,23
14202
PRIMER_RIGHT=213,20
14203
PRIMER_LEFT_TM=59.048
14204
PRIMER_RIGHT_TM=60.228
14205
PRIMER_LEFT_GC_PERCENT=47.826
14206
PRIMER_RIGHT_GC_PERCENT=50.000
14207
PRIMER_LEFT_SELF_ANY=7.00
14208
PRIMER_RIGHT_SELF_ANY=4.00
14209
PRIMER_LEFT_SELF_END=5.00
14210
PRIMER_RIGHT_SELF_END=2.00
14211
PRIMER_LEFT_END_STABILITY=8.2000
14212
PRIMER_RIGHT_END_STABILITY=6.2000
14213
PRIMER_PAIR_COMPL_ANY=5.00
14214
PRIMER_PAIR_COMPL_END=5.00
14215
PRIMER_PRODUCT_SIZE=175
14216
PRIMER_PAIR_PENALTY_1=4.2916
14217
PRIMER_LEFT_1_PENALTY=1.478935
14218
PRIMER_RIGHT_1_PENALTY=2.812624
14219
PRIMER_LEFT_1_SEQUENCE=TGGTTAGCAAGCAACACTGAA
14220
PRIMER_RIGHT_1_SEQUENCE=TTTCACAGCCTCCTCTTCTTT
14221
PRIMER_LEFT_1=28,21
14222
PRIMER_RIGHT_1=194,21
14223
PRIMER_LEFT_1_TM=59.521
14224
PRIMER_RIGHT_1_TM=58.187
14225
PRIMER_LEFT_1_GC_PERCENT=42.857
14226
PRIMER_RIGHT_1_GC_PERCENT=42.857
14227
PRIMER_LEFT_1_SELF_ANY=4.00
14228
PRIMER_RIGHT_1_SELF_ANY=3.00
14229
PRIMER_LEFT_1_SELF_END=0.00
14230
PRIMER_RIGHT_1_SELF_END=0.00
14231
PRIMER_LEFT_1_END_STABILITY=7.0000
14232
PRIMER_RIGHT_1_END_STABILITY=7.0000
14233
PRIMER_PAIR_1_COMPL_ANY=4.00
14234
PRIMER_PAIR_1_COMPL_END=2.00
14235
PRIMER_PRODUCT_SIZE_1=167
14236
PRIMER_PAIR_PENALTY_2=4.5577
14237
PRIMER_LEFT_2_PENALTY=3.855493
14238
PRIMER_RIGHT_2_PENALTY=0.702248
14239
PRIMER_LEFT_2_SEQUENCE=GCAACACTGAAGAGACTTGTCA
14240
PRIMER_RIGHT_2_SEQUENCE=CCCCTGCTCGCTCTTAAATT
14241
PRIMER_LEFT_2=38,22
14242
PRIMER_RIGHT_2=212,20
14243
PRIMER_LEFT_2_TM=58.145
14244
PRIMER_RIGHT_2_TM=60.702
14245
PRIMER_LEFT_2_GC_PERCENT=45.455
14246
PRIMER_RIGHT_2_GC_PERCENT=50.000
14247
PRIMER_LEFT_2_SELF_ANY=5.00
14248
PRIMER_RIGHT_2_SELF_ANY=4.00
14249
PRIMER_LEFT_2_SELF_END=5.00
14250
PRIMER_RIGHT_2_SELF_END=4.00
14251
PRIMER_LEFT_2_END_STABILITY=6.7000
14252
PRIMER_RIGHT_2_END_STABILITY=7.2000
14253
PRIMER_PAIR_2_COMPL_ANY=5.00
14254
PRIMER_PAIR_2_COMPL_END=2.00
14255
PRIMER_PRODUCT_SIZE_2=175
14256
PRIMER_PAIR_PENALTY_3=4.5794
14257
PRIMER_LEFT_3_PENALTY=1.478935
14258
PRIMER_RIGHT_3_PENALTY=3.100425
14259
PRIMER_LEFT_3_SEQUENCE=TGGTTAGCAAGCAACACTGAA
14260
PRIMER_RIGHT_3_SEQUENCE=CACAGCCTCCTCTTCTTTAATCA
14261
PRIMER_LEFT_3=28,21
14262
PRIMER_RIGHT_3=191,23
14263
PRIMER_LEFT_3_TM=59.521
14264
PRIMER_RIGHT_3_TM=59.900
14265
PRIMER_LEFT_3_GC_PERCENT=42.857
14266
PRIMER_RIGHT_3_GC_PERCENT=43.478
14267
PRIMER_LEFT_3_SELF_ANY=4.00
14268
PRIMER_RIGHT_3_SELF_ANY=4.00
14269
PRIMER_LEFT_3_SELF_END=0.00
14270
PRIMER_RIGHT_3_SELF_END=2.00
14271
PRIMER_LEFT_3_END_STABILITY=7.0000
14272
PRIMER_RIGHT_3_END_STABILITY=6.9000
14273
PRIMER_PAIR_3_COMPL_ANY=4.00
14274
PRIMER_PAIR_3_COMPL_END=2.00
14275
PRIMER_PRODUCT_SIZE_3=164
14276
PRIMER_PAIR_PENALTY_4=4.6541
14277
PRIMER_LEFT_4_PENALTY=3.951808
14278
PRIMER_RIGHT_4_PENALTY=0.702248
14279
PRIMER_LEFT_4_SEQUENCE=CAACACTGAAGAGACTTGTCAGG
14280
PRIMER_RIGHT_4_SEQUENCE=CCCCTGCTCGCTCTTAAATT
14281
PRIMER_LEFT_4=39,23
14282
PRIMER_RIGHT_4=212,20
14283
PRIMER_LEFT_4_TM=59.048
14284
PRIMER_RIGHT_4_TM=60.702
14285
PRIMER_LEFT_4_GC_PERCENT=47.826
14286
PRIMER_RIGHT_4_GC_PERCENT=50.000
14287
PRIMER_LEFT_4_SELF_ANY=7.00
14288
PRIMER_RIGHT_4_SELF_ANY=4.00
14289
PRIMER_LEFT_4_SELF_END=5.00
14290
PRIMER_RIGHT_4_SELF_END=4.00
14291
PRIMER_LEFT_4_END_STABILITY=8.2000
14292
PRIMER_RIGHT_4_END_STABILITY=7.2000
14293
PRIMER_PAIR_4_COMPL_ANY=5.00
14294
PRIMER_PAIR_4_COMPL_END=4.00
14295
PRIMER_PRODUCT_SIZE_4=174
14296
=
14297
MARKER_NAME=35.RHAP131FD5.seq
14298
SEQUENCE=NNNNNNNNNNNGNTTAAAATTTCAGTTGTCTTCACTTCACATCCAAGGCTGGATTTCTTGGGATGTGATACAGTGCGGATGGTGCTTGTGGGATCACACAATCTTATGAGACCTGTCTTTCTGTGTTGCCTGTAAACTGTTTCCTAAATANTTTGAGTAGATCTGTTACCTTCTAAATAAGGTTAGATCACTCAAATAACGTATTTGTCTCTAGCTTCTATCTCAGTCACATTTTAATTATTTATACCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNGTTTCCATTAACTTTGCCTTNTGANGANGACTCCTTTNTCTTTCANTCTNTTGGGNGGTANCGAAGCTCGNANTCGNNAATNGNNGTCNNNGNTGTTTTCCNGTGTNANNTTNNTNNCCGCNCACNATTCCANNCNNAATANGNNGCGGNAGGATANAAGTGGTANANCNGGGGGNNNNNAAANGNGTTGGGNNANTCAGNNTTANNNGGGGGGNNNNTNNCGNTTNNCAANNNGGGNAANNNNNNNTGNANNNGNNTTAATGNNTNGGGNNANG
14299
INCLUDED_REGION=11,568
14300
TARGET=250,50,TG
14301
PRIMER_COMMENT=CL is
14302
PRIMER_COMMENT=CR is
14303
=
14304
MARKER_NAME=38.RHAP131FE11.seq
14305
SEQUENCE=NNNNNNNNNNNNNNCCAATGAGCAACCCTGTCTTAAAACAACTGTGTGTGTTTTTCGGGGGGTCAAGGGGGCTGTTCCTGAGGAATGATTCAAGGTTGATCTCTGGCACCTGTGCTTGTGTGCACACATATGCACAAGTGTATGCACACATACACAACAGAATCTTCCAACACTTTGACCTTGGACTTTCCCTCTTTCAGAAGCAAGAGAAACAGATATCTGTTGTTTAAACCACTTAGTCTACAGTGATTGGTCACTTGGTGTCCTGTGCTATGAGGCGTGTGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTACTGAAATTAGAAACCACCCTTATCACCATGGTTTTCAGAGCCCAGTATCAGTTCAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
14306
INCLUDED_REGION=14,402
14307
TARGET=286,70,TG
14308
PRIMER_COMMENT=CL is CTGTGCTATGAGGCGTGTGT
14309
PRIMER_COMMENT=CR is ACTGGGCTCTGAAAACCATG
14310
PRIMER_PAIR_PENALTY=0.1822
14311
PRIMER_LEFT_PENALTY=0.071094
14312
PRIMER_RIGHT_PENALTY=0.111077
14313
PRIMER_LEFT_SEQUENCE=CTGTGCTATGAGGCGTGTGT
14314
PRIMER_RIGHT_SEQUENCE=ACTGGGCTCTGAAAACCATG
14315
PRIMER_LEFT=265,20
14316
PRIMER_RIGHT=404,20
14317
PRIMER_LEFT_TM=59.929
14318
PRIMER_RIGHT_TM=60.111
14319
PRIMER_LEFT_GC_PERCENT=55.000
14320
PRIMER_RIGHT_GC_PERCENT=50.000
14321
PRIMER_LEFT_SELF_ANY=2.00
14322
PRIMER_RIGHT_SELF_ANY=4.00
14323
PRIMER_LEFT_SELF_END=0.00
14324
PRIMER_RIGHT_SELF_END=4.00
14325
PRIMER_LEFT_END_STABILITY=6.4000
14326
PRIMER_RIGHT_END_STABILITY=8.4000
14327
PRIMER_PAIR_COMPL_ANY=3.00
14328
PRIMER_PAIR_COMPL_END=2.00
14329
PRIMER_PRODUCT_SIZE=140
14330
PRIMER_PAIR_PENALTY_1=0.4100
14331
PRIMER_LEFT_1_PENALTY=0.298942
14332
PRIMER_RIGHT_1_PENALTY=0.111077
14333
PRIMER_LEFT_1_SEQUENCE=TGGTGTCCTGTGCTATGAGG
14334
PRIMER_RIGHT_1_SEQUENCE=ACTGGGCTCTGAAAACCATG
14335
PRIMER_LEFT_1=258,20
14336
PRIMER_RIGHT_1=404,20
14337
PRIMER_LEFT_1_TM=59.701
14338
PRIMER_RIGHT_1_TM=60.111
14339
PRIMER_LEFT_1_GC_PERCENT=55.000
14340
PRIMER_RIGHT_1_GC_PERCENT=50.000
14341
PRIMER_LEFT_1_SELF_ANY=3.00
14342
PRIMER_RIGHT_1_SELF_ANY=4.00
14343
PRIMER_LEFT_1_SELF_END=0.00
14344
PRIMER_RIGHT_1_SELF_END=4.00
14345
PRIMER_LEFT_1_END_STABILITY=8.2000
14346
PRIMER_RIGHT_1_END_STABILITY=8.4000
14347
PRIMER_PAIR_1_COMPL_ANY=5.00
14348
PRIMER_PAIR_1_COMPL_END=1.00
14349
PRIMER_PRODUCT_SIZE_1=147
14350
PRIMER_PAIR_PENALTY_2=0.5448
14351
PRIMER_LEFT_2_PENALTY=0.071094
14352
PRIMER_RIGHT_2_PENALTY=0.473695
14353
PRIMER_LEFT_2_SEQUENCE=CTGTGCTATGAGGCGTGTGT
14354
PRIMER_RIGHT_2_SEQUENCE=CCATGGTGATAAGGGTGGTT
14355
PRIMER_LEFT_2=265,20
14356
PRIMER_RIGHT_2=389,20
14357
PRIMER_LEFT_2_TM=59.929
14358
PRIMER_RIGHT_2_TM=59.526
14359
PRIMER_LEFT_2_GC_PERCENT=55.000
14360
PRIMER_RIGHT_2_GC_PERCENT=50.000
14361
PRIMER_LEFT_2_SELF_ANY=2.00
14362
PRIMER_RIGHT_2_SELF_ANY=6.00
14363
PRIMER_LEFT_2_SELF_END=0.00
14364
PRIMER_RIGHT_2_SELF_END=0.00
14365
PRIMER_LEFT_2_END_STABILITY=6.4000
14366
PRIMER_RIGHT_2_END_STABILITY=8.2000
14367
PRIMER_PAIR_2_COMPL_ANY=3.00
14368
PRIMER_PAIR_2_COMPL_END=0.00
14369
PRIMER_PRODUCT_SIZE_2=125
14370
PRIMER_PAIR_PENALTY_3=0.5448
14371
PRIMER_LEFT_3_PENALTY=0.071094
14372
PRIMER_RIGHT_3_PENALTY=0.473695
14373
PRIMER_LEFT_3_SEQUENCE=CTGTGCTATGAGGCGTGTGT
14374
PRIMER_RIGHT_3_SEQUENCE=AACCATGGTGATAAGGGTGG
14375
PRIMER_LEFT_3=265,20
14376
PRIMER_RIGHT_3=391,20
14377
PRIMER_LEFT_3_TM=59.929
14378
PRIMER_RIGHT_3_TM=59.526
14379
PRIMER_LEFT_3_GC_PERCENT=55.000
14380
PRIMER_RIGHT_3_GC_PERCENT=50.000
14381
PRIMER_LEFT_3_SELF_ANY=2.00
14382
PRIMER_RIGHT_3_SELF_ANY=8.00
14383
PRIMER_LEFT_3_SELF_END=0.00
14384
PRIMER_RIGHT_3_SELF_END=2.00
14385
PRIMER_LEFT_3_END_STABILITY=6.4000
14386
PRIMER_RIGHT_3_END_STABILITY=9.4000
14387
PRIMER_PAIR_3_COMPL_ANY=3.00
14388
PRIMER_PAIR_3_COMPL_END=0.00
14389
PRIMER_PRODUCT_SIZE_3=127
14390
PRIMER_PAIR_PENALTY_4=0.7377
14391
PRIMER_LEFT_4_PENALTY=0.626619
14392
PRIMER_RIGHT_4_PENALTY=0.111077
14393
PRIMER_LEFT_4_SEQUENCE=TGGTCACTTGGTGTCCTGTG
14394
PRIMER_RIGHT_4_SEQUENCE=ACTGGGCTCTGAAAACCATG
14395
PRIMER_LEFT_4=250,20
14396
PRIMER_RIGHT_4=404,20
14397
PRIMER_LEFT_4_TM=60.627
14398
PRIMER_RIGHT_4_TM=60.111
14399
PRIMER_LEFT_4_GC_PERCENT=55.000
14400
PRIMER_RIGHT_4_GC_PERCENT=50.000
14401
PRIMER_LEFT_4_SELF_ANY=5.00
14402
PRIMER_RIGHT_4_SELF_ANY=4.00
14403
PRIMER_LEFT_4_SELF_END=1.00
14404
PRIMER_RIGHT_4_SELF_END=4.00
14405
PRIMER_LEFT_4_END_STABILITY=6.7000
14406
PRIMER_RIGHT_4_END_STABILITY=8.4000
14407
PRIMER_PAIR_4_COMPL_ANY=4.00
14408
PRIMER_PAIR_4_COMPL_END=4.00
14409
PRIMER_PRODUCT_SIZE_4=155
14410
=
14411
MARKER_NAME=38.RHAP131FE5.seq
14412
SEQUENCE=NNNNNNNNNCANCTCCGAAAAAAANNCCAAAACTAGTTCTCAGCTCTAGATAAAGACTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGCCTGCCTTACCTTGATCCTGACGCCCTTGTGTCATTTGTAGGTCAACCAACCACCCATACGTTTCAAGCAATAAAGAATCCAGAGAAAATAATCACNNNNNNNNNNNNNNNNNNGAGTTTTCTGACAGATGCCCAAAGATGCTTTCCAAAGCGTATGTTAGAAAGTGTGAACGAATGAATTCCTCTCTCCCTTCCCGCCCTAAAACACGAACTCACATTCTCCCAGAGATCCACTCCACTCTCCTGTTCCTTCTGTGATAGTTAATTTTGTCTCTTAGCATCACTAGATTTCCAGTCACCTAAGAAACATACCCCTGGGTGTGTGTGTCTTTTGGGGTGATCCTGCAAAAGGTTTAACTGAGGAGTGGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
14413
INCLUDED_REGION=9,456
14414
TARGET=59,32,TG
14415
TARGET=190,18,CT
14416
PRIMER_COMMENT=CL is CTTACCTTGATCCTGACGCC
14417
PRIMER_COMMENT=CR is GCTTTGGAAAGCATCTTTGG
14418
PRIMER_PAIR_PENALTY=0.4832
14419
PRIMER_LEFT_PENALTY=0.306150
14420
PRIMER_RIGHT_PENALTY=0.177008
14421
PRIMER_LEFT_SEQUENCE=GCCTTACCTTGATCCTGACG
14422
PRIMER_RIGHT_SEQUENCE=GCTTTGGAAAGCATCTTTGG
14423
PRIMER_LEFT=97,20
14424
PRIMER_RIGHT=245,20
14425
PRIMER_LEFT_TM=59.694
14426
PRIMER_RIGHT_TM=59.823
14427
PRIMER_LEFT_GC_PERCENT=55.000
14428
PRIMER_RIGHT_GC_PERCENT=45.000
14429
PRIMER_LEFT_SELF_ANY=4.00
14430
PRIMER_RIGHT_SELF_ANY=8.00
14431
PRIMER_LEFT_SELF_END=2.00
14432
PRIMER_RIGHT_SELF_END=2.00
14433
PRIMER_LEFT_END_STABILITY=8.4000
14434
PRIMER_RIGHT_END_STABILITY=8.8000
14435
PRIMER_PAIR_COMPL_ANY=4.00
14436
PRIMER_PAIR_COMPL_END=0.00
14437
PRIMER_PRODUCT_SIZE=149
14438
PRIMER_PAIR_PENALTY_1=0.4832
14439
PRIMER_LEFT_1_PENALTY=0.306150
14440
PRIMER_RIGHT_1_PENALTY=0.177008
14441
PRIMER_LEFT_1_SEQUENCE=CCTTACCTTGATCCTGACGC
14442
PRIMER_RIGHT_1_SEQUENCE=GCTTTGGAAAGCATCTTTGG
14443
PRIMER_LEFT_1=98,20
14444
PRIMER_RIGHT_1=245,20
14445
PRIMER_LEFT_1_TM=59.694
14446
PRIMER_RIGHT_1_TM=59.823
14447
PRIMER_LEFT_1_GC_PERCENT=55.000
14448
PRIMER_RIGHT_1_GC_PERCENT=45.000
14449
PRIMER_LEFT_1_SELF_ANY=4.00
14450
PRIMER_RIGHT_1_SELF_ANY=8.00
14451
PRIMER_LEFT_1_SELF_END=2.00
14452
PRIMER_RIGHT_1_SELF_END=2.00
14453
PRIMER_LEFT_1_END_STABILITY=9.6000
14454
PRIMER_RIGHT_1_END_STABILITY=8.8000
14455
PRIMER_PAIR_1_COMPL_ANY=4.00
14456
PRIMER_PAIR_1_COMPL_END=1.00
14457
PRIMER_PRODUCT_SIZE_1=148
14458
PRIMER_PAIR_PENALTY_2=0.4832
14459
PRIMER_LEFT_2_PENALTY=0.306150
14460
PRIMER_RIGHT_2_PENALTY=0.177008
14461
PRIMER_LEFT_2_SEQUENCE=CTTACCTTGATCCTGACGCC
14462
PRIMER_RIGHT_2_SEQUENCE=GCTTTGGAAAGCATCTTTGG
14463
PRIMER_LEFT_2=99,20
14464
PRIMER_RIGHT_2=245,20
14465
PRIMER_LEFT_2_TM=59.694
14466
PRIMER_RIGHT_2_TM=59.823
14467
PRIMER_LEFT_2_GC_PERCENT=55.000
14468
PRIMER_RIGHT_2_GC_PERCENT=45.000
14469
PRIMER_LEFT_2_SELF_ANY=4.00
14470
PRIMER_RIGHT_2_SELF_ANY=8.00
14471
PRIMER_LEFT_2_SELF_END=2.00
14472
PRIMER_RIGHT_2_SELF_END=2.00
14473
PRIMER_LEFT_2_END_STABILITY=11.1000
14474
PRIMER_RIGHT_2_END_STABILITY=8.8000
14475
PRIMER_PAIR_2_COMPL_ANY=4.00
14476
PRIMER_PAIR_2_COMPL_END=2.00
14477
PRIMER_PRODUCT_SIZE_2=147
14478
PRIMER_PAIR_PENALTY_3=0.4890
14479
PRIMER_LEFT_3_PENALTY=0.312010
14480
PRIMER_RIGHT_3_PENALTY=0.177008
14481
PRIMER_LEFT_3_SEQUENCE=CCTGCCTTACCTTGATCCTG
14482
PRIMER_RIGHT_3_SEQUENCE=GCTTTGGAAAGCATCTTTGG
14483
PRIMER_LEFT_3=94,20
14484
PRIMER_RIGHT_3=245,20
14485
PRIMER_LEFT_3_TM=59.688
14486
PRIMER_RIGHT_3_TM=59.823
14487
PRIMER_LEFT_3_GC_PERCENT=55.000
14488
PRIMER_RIGHT_3_GC_PERCENT=45.000
14489
PRIMER_LEFT_3_SELF_ANY=4.00
14490
PRIMER_RIGHT_3_SELF_ANY=8.00
14491
PRIMER_LEFT_3_SELF_END=2.00
14492
PRIMER_RIGHT_3_SELF_END=2.00
14493
PRIMER_LEFT_3_END_STABILITY=8.2000
14494
PRIMER_RIGHT_3_END_STABILITY=8.8000
14495
PRIMER_PAIR_3_COMPL_ANY=4.00
14496
PRIMER_PAIR_3_COMPL_END=1.00
14497
PRIMER_PRODUCT_SIZE_3=152
14498
PRIMER_PAIR_PENALTY_4=0.5197
14499
PRIMER_LEFT_4_PENALTY=0.306150
14500
PRIMER_RIGHT_4_PENALTY=0.213506
14501
PRIMER_LEFT_4_SEQUENCE=GCCTTACCTTGATCCTGACG
14502
PRIMER_RIGHT_4_SEQUENCE=AAAGCATCTTTGGGCATCTG
14503
PRIMER_LEFT_4=97,20
14504
PRIMER_RIGHT_4=238,20
14505
PRIMER_LEFT_4_TM=59.694
14506
PRIMER_RIGHT_4_TM=60.214
14507
PRIMER_LEFT_4_GC_PERCENT=55.000
14508
PRIMER_RIGHT_4_GC_PERCENT=45.000
14509
PRIMER_LEFT_4_SELF_ANY=4.00
14510
PRIMER_RIGHT_4_SELF_ANY=6.00
14511
PRIMER_LEFT_4_SELF_END=2.00
14512
PRIMER_RIGHT_4_SELF_END=2.00
14513
PRIMER_LEFT_4_END_STABILITY=8.4000
14514
PRIMER_RIGHT_4_END_STABILITY=6.6000
14515
PRIMER_PAIR_4_COMPL_ANY=4.00
14516
PRIMER_PAIR_4_COMPL_END=2.00
14517
PRIMER_PRODUCT_SIZE_4=142
14518
=
14519
MARKER_NAME=40.RHAP131FF4.seq
14520
SEQUENCE=NNNNNNNNNNNNNNNNNNNNCAGTCACACAAAGATAGAGTATAGAATAGAGTTTATTTAGGGCATGGGGAGGAGAGTTGAAAGGCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGAGGAGTAGAGGCTGGTGGTGAGCACGTAGAGAGAAGGGAAGGGGCAGGGGAAGGAGAGAAAGGGAGAGCAGGAACAAGAGAGGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
14521
INCLUDED_REGION=20,212
14522
TARGET=86,58,GT
14523
PRIMER_COMMENT=CL is CATGGGGAGGAGAGTTGAAA
14524
PRIMER_COMMENT=CR is CCCCTTCCCTTCTCTCTACG
14525
PRIMER_PAIR_PENALTY=0.2389
14526
PRIMER_LEFT_PENALTY=0.042377
14527
PRIMER_RIGHT_PENALTY=0.196491
14528
PRIMER_LEFT_SEQUENCE=CATGGGGAGGAGAGTTGAAA
14529
PRIMER_RIGHT_SEQUENCE=CCCCTTCCCTTCTCTCTACG
14530
PRIMER_LEFT=62,20
14531
PRIMER_RIGHT=189,20
14532
PRIMER_LEFT_TM=60.042
14533
PRIMER_RIGHT_TM=60.196
14534
PRIMER_LEFT_GC_PERCENT=50.000
14535
PRIMER_RIGHT_GC_PERCENT=60.000
14536
PRIMER_LEFT_SELF_ANY=4.00
14537
PRIMER_RIGHT_SELF_ANY=2.00
14538
PRIMER_LEFT_SELF_END=2.00
14539
PRIMER_RIGHT_SELF_END=2.00
14540
PRIMER_LEFT_END_STABILITY=7.3000
14541
PRIMER_RIGHT_END_STABILITY=7.4000
14542
PRIMER_PAIR_COMPL_ANY=5.00
14543
PRIMER_PAIR_COMPL_END=2.00
14544
PRIMER_PRODUCT_SIZE=128
14545
PRIMER_PAIR_PENALTY_1=0.2936
14546
PRIMER_LEFT_1_PENALTY=0.042377
14547
PRIMER_RIGHT_1_PENALTY=0.251196
14548
PRIMER_LEFT_1_SEQUENCE=CATGGGGAGGAGAGTTGAAA
14549
PRIMER_RIGHT_1_SEQUENCE=TCTCCCTTTCTCTCCTTCCC
14550
PRIMER_LEFT_1=62,20
14551
PRIMER_RIGHT_1=212,20
14552
PRIMER_LEFT_1_TM=60.042
14553
PRIMER_RIGHT_1_TM=59.749
14554
PRIMER_LEFT_1_GC_PERCENT=50.000
14555
PRIMER_RIGHT_1_GC_PERCENT=55.000
14556
PRIMER_LEFT_1_SELF_ANY=4.00
14557
PRIMER_RIGHT_1_SELF_ANY=0.00
14558
PRIMER_LEFT_1_SELF_END=2.00
14559
PRIMER_RIGHT_1_SELF_END=0.00
14560
PRIMER_LEFT_1_END_STABILITY=7.3000
14561
PRIMER_RIGHT_1_END_STABILITY=9.7000
14562
PRIMER_PAIR_1_COMPL_ANY=9.00
14563
PRIMER_PAIR_1_COMPL_END=6.00
14564
PRIMER_PRODUCT_SIZE_1=151
14565
PRIMER_PAIR_PENALTY_2=0.5105
14566
PRIMER_LEFT_2_PENALTY=0.042377
14567
PRIMER_RIGHT_2_PENALTY=0.468086
14568
PRIMER_LEFT_2_SEQUENCE=CATGGGGAGGAGAGTTGAAA
14569
PRIMER_RIGHT_2_SEQUENCE=CCTGCTCTCCCTTTCTCTCC
14570
PRIMER_LEFT_2=62,20
14571
PRIMER_RIGHT_2=217,20
14572
PRIMER_LEFT_2_TM=60.042
14573
PRIMER_RIGHT_2_TM=60.468
14574
PRIMER_LEFT_2_GC_PERCENT=50.000
14575
PRIMER_RIGHT_2_GC_PERCENT=60.000
14576
PRIMER_LEFT_2_SELF_ANY=4.00
14577
PRIMER_RIGHT_2_SELF_ANY=2.00
14578
PRIMER_LEFT_2_SELF_END=2.00
14579
PRIMER_RIGHT_2_SELF_END=0.00
14580
PRIMER_LEFT_2_END_STABILITY=7.3000
14581
PRIMER_RIGHT_2_END_STABILITY=7.9000
14582
PRIMER_PAIR_2_COMPL_ANY=6.00
14583
PRIMER_PAIR_2_COMPL_END=4.00
14584
PRIMER_PRODUCT_SIZE_2=156
14585
PRIMER_PAIR_PENALTY_3=0.5124
14586
PRIMER_LEFT_3_PENALTY=0.042377
14587
PRIMER_RIGHT_3_PENALTY=0.470001
14588
PRIMER_LEFT_3_SEQUENCE=CATGGGGAGGAGAGTTGAAA
14589
PRIMER_RIGHT_3_SEQUENCE=TCTCTTGTTCCTGCTCTCCC
14590
PRIMER_LEFT_3=62,20
14591
PRIMER_RIGHT_3=226,20
14592
PRIMER_LEFT_3_TM=60.042
14593
PRIMER_RIGHT_3_TM=59.530
14594
PRIMER_LEFT_3_GC_PERCENT=50.000
14595
PRIMER_RIGHT_3_GC_PERCENT=55.000
14596
PRIMER_LEFT_3_SELF_ANY=4.00
14597
PRIMER_RIGHT_3_SELF_ANY=2.00
14598
PRIMER_LEFT_3_SELF_END=2.00
14599
PRIMER_RIGHT_3_SELF_END=0.00
14600
PRIMER_LEFT_3_END_STABILITY=7.3000
14601
PRIMER_RIGHT_3_END_STABILITY=9.4000
14602
PRIMER_PAIR_3_COMPL_ANY=6.00
14603
PRIMER_PAIR_3_COMPL_END=5.00
14604
PRIMER_PRODUCT_SIZE_3=165
14605
PRIMER_PAIR_PENALTY_4=0.6371
14606
PRIMER_LEFT_4_PENALTY=0.042377
14607
PRIMER_RIGHT_4_PENALTY=0.594713
14608
PRIMER_LEFT_4_SEQUENCE=CATGGGGAGGAGAGTTGAAA
14609
PRIMER_RIGHT_4_SEQUENCE=TTGTTCCTGCTCTCCCTTTC
14610
PRIMER_LEFT_4=62,20
14611
PRIMER_RIGHT_4=222,20
14612
PRIMER_LEFT_4_TM=60.042
14613
PRIMER_RIGHT_4_TM=59.405
14614
PRIMER_LEFT_4_GC_PERCENT=50.000
14615
PRIMER_RIGHT_4_GC_PERCENT=50.000
14616
PRIMER_LEFT_4_SELF_ANY=4.00
14617
PRIMER_RIGHT_4_SELF_ANY=2.00
14618
PRIMER_LEFT_4_SELF_END=2.00
14619
PRIMER_RIGHT_4_SELF_END=0.00
14620
PRIMER_LEFT_4_END_STABILITY=7.3000
14621
PRIMER_RIGHT_4_END_STABILITY=7.0000
14622
PRIMER_PAIR_4_COMPL_ANY=6.00
14623
PRIMER_PAIR_4_COMPL_END=4.00
14624
PRIMER_PRODUCT_SIZE_4=161
14625
=
14626
MARKER_NAME=43.RHAP131FG10.seq
14627
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNNNCCATACACAGAACTAGGGTCTATACACAGTACTAGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGTACAGACATGAATGCATAGTGTAGGCATAAAAAGAAAGGCTCGATTTCATTCTTTTATCCACACTGTTCACCTGTGTCACCTGTTTTGAGCATGTGAGTCTGTTTTGAGCATGCTAGCCTGTATATAACTAGAGGAACTTAACAAGACTGCACAAGTGAACTCAGGGTAAGGACATATATAAAAGAACATTACAATAGGCAGGGTGAAGTGGGAACAGCAATCCCAGAACACATTCTGGAAATGTTGTAGTTTGAGGAGGAAGAGAGGGAAAGGTCACTCTGGGCATAATTTGGCTTTAACATCAATTTTCCTTCTGGTAGGAGTTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
14628
INCLUDED_REGION=23,414
14629
TARGET=60,46,TG
14630
PRIMER_COMMENT=CL is
14631
PRIMER_COMMENT=CR is
14632
=
14633
MARKER_NAME=47.RHAP131FH5.seq
14634
SEQUENCE=ANNTNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGTTNGTGTCTGGGGGGAGGCTNCTCTCCTGTGTGNTGCAGCCANTGGGGTTGGGGAGGAGCANANAGTTTGCNTTNNTGGAGTGAATCAACCAAATTTTGGGAGTTGGAAGTATTTTATTANAAACATNTGGAGATTTTAGAATTTTTTNAAAATGTTGTTATTTAAANCCTTTTCAACGTTAGAAAGTAATAAGTAGGGGTGGGNGGNACGCCCTCNNAGGNNNTTGTTTCAAAANGNNTCNCCCAACTCCCATGANTTTCTTGGNTTTTTTTNNTTNCCGGNAACCNTGNTCTCCTNNAANATCAAAACCCATCAACTTTTGGNGGCCANAATAATCNNTTTTTACTANANTGNNTGNGNGTCCCTTGGTANANTNGNTTNNGGGGGTANCNGANCTCNANNTTCNNTAANTNATGGGNNAAANNGGTTTTTTTNCNTGGGGNNNANTTGTTTTNNTCTNNCCNNNNATTTTTNCNACATTGANANNNNGGNNCCCGGGGCGCNATTNNNGGTGTAANNNCCTGGGGGNNNNNTAANNGNGNGGNNNNNNCCTCANTTTTTANTGGGCGGTNNNCCNCNNNTGCCCNTTTTTTCTGNNTGGGGAAANNTTGTTNNNNNNNNTNG
14635
INCLUDED_REGION=0,663
14636
TARGET=6,29,GT
14637
PRIMER_COMMENT=CL is
14638
PRIMER_COMMENT=CR is
14639
=
14640
MARKER_NAME=48.RHAP131FH12.seq
14641
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCAGGCATGNGTGAGGGGATGAGAACCCACCTGCAATGTNAAGGAAGTGATGTGATTATTGTTCATGCCCATGTCTCATTCTTCTCCCTTTCCCATGTTGGTCCTGATGGAAATGGAAAAAATAGAGTTGGGGGAAACTTGGAGACCTTATTGTAATATTAGAGACTATGAGGAATCTTAGGATTCTTTTGGGAAAGGCTTGGGCATGTCTTATGATAGATTTCAAGCTAGTAACAACACATCCAAGGATGCTTCTTAAGAAATTTAATTAATAAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTACATTCAGGTCAACATAGTTGCTTGTGGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
14642
INCLUDED_REGION=21,348
14643
TARGET=297,40,TG
14644
PRIMER_COMMENT=CL is AGGCTTGGGCATGTCTTATG
14645
PRIMER_COMMENT=CR is TCCACAAGCAACTATGTTGACC
14646
PRIMER_PAIR_PENALTY=2.1331
14647
PRIMER_LEFT_PENALTY=0.095771
14648
PRIMER_RIGHT_PENALTY=2.037339
14649
PRIMER_LEFT_SEQUENCE=AGGCTTGGGCATGTCTTATG
14650
PRIMER_RIGHT_SEQUENCE=TCCACAAGCAACTATGTTGACC
14651
PRIMER_LEFT=215,20
14652
PRIMER_RIGHT=366,22
14653
PRIMER_LEFT_TM=60.096
14654
PRIMER_RIGHT_TM=60.037
14655
PRIMER_LEFT_GC_PERCENT=50.000
14656
PRIMER_RIGHT_GC_PERCENT=45.455
14657
PRIMER_LEFT_SELF_ANY=4.00
14658
PRIMER_RIGHT_SELF_ANY=6.00
14659
PRIMER_LEFT_SELF_END=2.00
14660
PRIMER_RIGHT_SELF_END=2.00
14661
PRIMER_LEFT_END_STABILITY=6.2000
14662
PRIMER_RIGHT_END_STABILITY=7.9000
14663
PRIMER_PAIR_COMPL_ANY=5.00
14664
PRIMER_PAIR_COMPL_END=1.00
14665
PRIMER_PRODUCT_SIZE=152
14666
PRIMER_PAIR_PENALTY_1=2.2639
14667
PRIMER_LEFT_1_PENALTY=0.095771
14668
PRIMER_RIGHT_1_PENALTY=2.168114
14669
PRIMER_LEFT_1_SEQUENCE=AGGCTTGGGCATGTCTTATG
14670
PRIMER_RIGHT_1_SEQUENCE=CCTCCACAAGCAACTATGTTGA
14671
PRIMER_LEFT_1=215,20
14672
PRIMER_RIGHT_1=368,22
14673
PRIMER_LEFT_1_TM=60.096
14674
PRIMER_RIGHT_1_TM=60.168
14675
PRIMER_LEFT_1_GC_PERCENT=50.000
14676
PRIMER_RIGHT_1_GC_PERCENT=45.455
14677
PRIMER_LEFT_1_SELF_ANY=4.00
14678
PRIMER_RIGHT_1_SELF_ANY=6.00
14679
PRIMER_LEFT_1_SELF_END=2.00
14680
PRIMER_RIGHT_1_SELF_END=5.00
14681
PRIMER_LEFT_1_END_STABILITY=6.2000
14682
PRIMER_RIGHT_1_END_STABILITY=6.7000
14683
PRIMER_PAIR_1_COMPL_ANY=5.00
14684
PRIMER_PAIR_1_COMPL_END=1.00
14685
PRIMER_PRODUCT_SIZE_1=154
14686
PRIMER_PAIR_PENALTY_2=2.3298
14687
PRIMER_LEFT_2_PENALTY=0.292414
14688
PRIMER_RIGHT_2_PENALTY=2.037339
14689
PRIMER_LEFT_2_SEQUENCE=AAAGGCTTGGGCATGTCTTA
14690
PRIMER_RIGHT_2_SEQUENCE=TCCACAAGCAACTATGTTGACC
14691
PRIMER_LEFT_2=213,20
14692
PRIMER_RIGHT_2=366,22
14693
PRIMER_LEFT_2_TM=59.708
14694
PRIMER_RIGHT_2_TM=60.037
14695
PRIMER_LEFT_2_GC_PERCENT=45.000
14696
PRIMER_RIGHT_2_GC_PERCENT=45.455
14697
PRIMER_LEFT_2_SELF_ANY=6.00
14698
PRIMER_RIGHT_2_SELF_ANY=6.00
14699
PRIMER_LEFT_2_SELF_END=4.00
14700
PRIMER_RIGHT_2_SELF_END=2.00
14701
PRIMER_LEFT_2_END_STABILITY=6.0000
14702
PRIMER_RIGHT_2_END_STABILITY=7.9000
14703
PRIMER_PAIR_2_COMPL_ANY=5.00
14704
PRIMER_PAIR_2_COMPL_END=0.00
14705
PRIMER_PRODUCT_SIZE_2=154
14706
PRIMER_PAIR_PENALTY_3=2.4605
14707
PRIMER_LEFT_3_PENALTY=0.292414
14708
PRIMER_RIGHT_3_PENALTY=2.168114
14709
PRIMER_LEFT_3_SEQUENCE=AAAGGCTTGGGCATGTCTTA
14710
PRIMER_RIGHT_3_SEQUENCE=CCTCCACAAGCAACTATGTTGA
14711
PRIMER_LEFT_3=213,20
14712
PRIMER_RIGHT_3=368,22
14713
PRIMER_LEFT_3_TM=59.708
14714
PRIMER_RIGHT_3_TM=60.168
14715
PRIMER_LEFT_3_GC_PERCENT=45.000
14716
PRIMER_RIGHT_3_GC_PERCENT=45.455
14717
PRIMER_LEFT_3_SELF_ANY=6.00
14718
PRIMER_RIGHT_3_SELF_ANY=6.00
14719
PRIMER_LEFT_3_SELF_END=4.00
14720
PRIMER_RIGHT_3_SELF_END=5.00
14721
PRIMER_LEFT_3_END_STABILITY=6.0000
14722
PRIMER_RIGHT_3_END_STABILITY=6.7000
14723
PRIMER_PAIR_3_COMPL_ANY=5.00
14724
PRIMER_PAIR_3_COMPL_END=1.00
14725
PRIMER_PRODUCT_SIZE_3=156
14726
PRIMER_PAIR_PENALTY_4=2.5052
14727
PRIMER_LEFT_4_PENALTY=0.467882
14728
PRIMER_RIGHT_4_PENALTY=2.037339
14729
PRIMER_LEFT_4_SEQUENCE=AGGATTCTTTTGGGAAAGGC
14730
PRIMER_RIGHT_4_SEQUENCE=TCCACAAGCAACTATGTTGACC
14731
PRIMER_LEFT_4=199,20
14732
PRIMER_RIGHT_4=366,22
14733
PRIMER_LEFT_4_TM=59.532
14734
PRIMER_RIGHT_4_TM=60.037
14735
PRIMER_LEFT_4_GC_PERCENT=45.000
14736
PRIMER_RIGHT_4_GC_PERCENT=45.455
14737
PRIMER_LEFT_4_SELF_ANY=4.00
14738
PRIMER_RIGHT_4_SELF_ANY=6.00
14739
PRIMER_LEFT_4_SELF_END=2.00
14740
PRIMER_RIGHT_4_SELF_END=2.00
14741
PRIMER_LEFT_4_END_STABILITY=9.7000
14742
PRIMER_RIGHT_4_END_STABILITY=7.9000
14743
PRIMER_PAIR_4_COMPL_ANY=5.00
14744
PRIMER_PAIR_4_COMPL_END=1.00
14745
PRIMER_PRODUCT_SIZE_4=168
14746
=
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