1
ACCEPTABLE LEFT PRIMERS
2
0-based # self self lib qual-
3
# sequence start ln N GC% Tm any end sim lity
4
0 TCGCAATCAAATTTTTTCTATGACCTG 36 27 0 33.33 65.188 6.00 1.00 9.00 12.188
5
1 TCGCAATCAAATTTTTTCTATGACCT 36 26 0 30.77 63.123 6.00 1.00 10.00 9.123
6
2 ATCGCAATCAAATTTTTTCTATGACCT 35 27 0 29.63 63.204 6.00 1.00 10.00 10.204
7
3 TCGCAATCAAATTTTTTCTATGACC 36 25 0 32.00 62.463 6.00 0.00 11.00 7.463
8
4 ATCGCAATCAAATTTTTTCTATGACC 35 26 0 30.77 62.574 6.00 0.00 11.00 8.574
9
5 AATCGCAATCAAATTTTTTCTATGACC 34 27 0 29.63 63.467 6.00 0.00 11.00 10.467
10
6 TCGCAATCAAATTTTTTCTATGAC 36 24 0 29.17 59.542 6.00 1.00 10.00 4.458
11
7 ATCGCAATCAAATTTTTTCTATGAC 35 25 0 28.00 59.789 6.00 2.00 10.00 5.211
12
8 AATCGCAATCAAATTTTTTCTATGAC 34 26 0 26.92 60.843 6.00 1.00 10.00 6.843
13
9 GAATCGCAATCAAATTTTTTCTATGAC 33 27 0 29.63 62.235 6.00 1.00 10.00 9.235
14
10 TCGCAATCAAATTTTTTCTATGA 36 23 0 26.09 58.793 6.00 2.00 10.00 4.207
15
11 AATCGCAATCAAATTTTTTCTATGA 34 25 0 24.00 60.202 6.00 2.00 10.00 5.202
16
12 GAATCGCAATCAAATTTTTTCTATGA 33 26 0 26.92 61.653 6.00 2.00 10.00 7.653
17
13 TGAATCGCAATCAAATTTTTTCTATGA 32 27 0 25.93 63.763 6.00 2.00 11.00 10.763
18
14 TCGCAATCAAATTTTTTCTATG 36 22 0 27.27 57.077 6.00 2.00 10.00 4.923
19
15 ATCGCAATCAAATTTTTTCTATG 35 23 0 26.09 57.456 6.00 2.00 10.00 5.544
20
16 AATCGCAATCAAATTTTTTCTATG 34 24 0 25.00 58.677 6.00 2.00 10.00 5.323
21
17 GAATCGCAATCAAATTTTTTCTATG 33 25 0 28.00 60.202 6.00 2.00 10.00 5.202
22
18 TGAATCGCAATCAAATTTTTTCTATG 32 26 0 26.92 62.397 6.00 2.00 10.00 8.397
23
19 ATGAATCGCAATCAAATTTTTTCTATG 31 27 0 25.93 62.507 6.00 2.00 10.00 9.507
24
20 TCGCAATCAAATTTTTTCTAT 36 21 0 23.81 54.389 6.00 2.00 9.00 6.611
25
21 TCGCAATCAAATTTTTTCTA 36 20 0 25.00 53.814 6.00 2.00 9.00 6.186
26
22 ATCGCAATCAAATTTTTTCTA 35 21 0 23.81 54.389 6.00 2.00 9.00 6.611
27
23 AATCGCAATCAAATTTTTTCTA 34 22 0 22.73 55.850 6.00 2.00 9.00 6.150
28
24 GAATCGCAATCAAATTTTTTCTA 33 23 0 26.09 57.561 6.00 2.00 9.00 5.439
29
25 TGAATCGCAATCAAATTTTTTCTA 32 24 0 25.00 59.977 6.00 2.00 10.00 4.023
30
26 ATGAATCGCAATCAAATTTTTTCTA 31 25 0 24.00 60.202 6.00 2.00 10.00 5.202
31
27 TATGAATCGCAATCAAATTTTTTCTA 30 26 0 23.08 60.204 6.00 2.00 10.00 6.204
32
28 TTATGAATCGCAATCAAATTTTTTCTA 29 27 0 22.22 61.199 6.00 2.00 10.00 8.199
33
29 TCGCAATCAAATTTTTTCT 36 19 0 26.32 53.559 6.00 2.00 10.00 7.441
34
30 ATCGCAATCAAATTTTTTCT 35 20 0 25.00 54.168 6.00 2.00 10.00 5.832
35
31 AATCGCAATCAAATTTTTTCT 34 21 0 23.81 55.693 6.00 2.00 10.00 5.307
36
32 GAATCGCAATCAAATTTTTTCT 33 22 0 27.27 57.467 6.00 2.00 10.00 4.533
37
33 TGAATCGCAATCAAATTTTTTCT 32 23 0 26.09 59.967 6.00 2.00 10.00 3.033
38
34 ATGAATCGCAATCAAATTTTTTCT 31 24 0 25.00 60.199 6.00 2.00 10.00 4.199
39
35 TATGAATCGCAATCAAATTTTTTCT 30 25 0 24.00 60.202 6.00 2.00 10.00 5.202
40
36 TTATGAATCGCAATCAAATTTTTTCT 29 26 0 23.08 61.226 6.00 2.00 10.00 7.226
41
37 ATTATGAATCGCAATCAAATTTTTTCT 28 27 0 22.22 61.382 6.00 2.00 10.00 8.382
42
38 TCGCAATCAAATTTTTTC 36 18 0 27.78 52.197 6.00 2.00 9.00 9.803
43
39 ATCGCAATCAAATTTTTTC 35 19 0 26.32 52.911 6.00 2.00 9.00 8.089
44
40 AATCGCAATCAAATTTTTTC 34 20 0 25.00 54.571 6.00 2.00 9.00 5.429
45
41 GAATCGCAATCAAATTTTTTC 33 21 0 28.57 56.457 6.00 2.00 9.00 4.543
46
42 TGAATCGCAATCAAATTTTTTC 32 22 0 27.27 59.091 6.00 2.00 10.00 2.909
47
43 ATGAATCGCAATCAAATTTTTTC 31 23 0 26.09 59.374 6.00 2.00 10.00 3.626
48
44 TATGAATCGCAATCAAATTTTTTC 30 24 0 25.00 59.404 6.00 2.00 10.00 4.596
49
45 TTATGAATCGCAATCAAATTTTTTC 29 25 0 24.00 60.502 6.00 2.00 10.00 5.502
50
46 ATTATGAATCGCAATCAAATTTTTTC 28 26 0 23.08 60.694 6.00 2.00 10.00 6.694
51
47 AATTATGAATCGCAATCAAATTTTTTC 27 27 0 22.22 61.652 8.00 2.00 10.00 8.652
52
48 TATGAATCGCAATCAAAT 30 18 0 27.78 49.586 4.00 2.00 10.00 12.414
53
49 ATTATGAATCGCAATCAA 28 18 0 27.78 49.586 5.00 2.00 10.00 12.414
54
50 AATTATGAATCGCAATCAA 27 19 0 26.32 51.609 5.00 2.00 10.00 9.391
added
removed
test/primer_list_last/SP01-06-with-repeat1.for
