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Multiple alignment program of protein sequences
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Some information about Muscle from the Debian-Med project.
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MUSCLE is a multiple alignment program for protein sequences. MUSCLE
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stands for multiple sequence comparison by log-expectation. In the
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authors tests, MUSCLE achieved the highest scores of all tested
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programs on several alignment accuracy benchmarks, and is also one of
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the fastest programs out there.
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Homepage: http://www.drive5.com/muscle
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muscle -in <inputfile> -out <outputfile>
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Common options (for a complete list please see the User Guide):
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-in <inputfile> Input file in FASTA format (default stdin)
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-out <outputfile> Output alignment in FASTA format (default stdout)
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-diags Find diagonals (faster for similar sequences)
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-maxiters <n> Maximum number of iterations (integer, default 16)
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-maxhours <h> Maximum time to iterate in hours (default no limit)
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-maxmb <m> Maximum memory to allocate in Mb (default 80% of RAM)
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-html Write output in HTML format (default FASTA)
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-msf Write output in GCG MSF format (default FASTA)
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-clw Write output in CLUSTALW format (default FASTA)
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-clwstrict As -clw, with 'CLUSTAL W (1.81)' header
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-log[a] <logfile> Log to file (append if -loga, overwrite if -log)
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-quiet Do not write progress messages to stderr
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-stable Output sequences in input order (default is -group)
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-group Group sequences by similarity (this is the default)
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-version Display version information and exit
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Without refinement (very fast, avg accuracy similar to T-Coffee): -maxiters 2
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Fastest possible (amino acids): -maxiters 1 -diags -sv -distance1 kbit20_3
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Fastest possible (nucleotides): -maxiters 1 -diags