1
SEQUENCE=atgNaggtgacttttatttacatcctagttattacatgttacgaaaacgacgtaaacgtttaccatattttttttcagatgtctctttggctgcctagtgaggccactgtctacttgcctcctgtcccagtatctaaNgttgtaagcacggatgaatatgttgcacgcacaaacatatattatcatgcaggaacatccagactacttgcagttggacatccctattttcctattaaaaaacctaacaataacaaaatattagttcctaaagtatcaggattacaatacagggtatttagaataNatttacctgaccccttagtgaataagtttggttttcctgacacctcattttaNaatccagatacacagcggctggtttgggcctgtgtaggtgttgaggtaggNcgtggtcagccattaggtgtgggcattagtggccatcctttattaaataaattggatgacacagaaaatgctagtgcttatgcagcaaatgcaggtgtggataatagagaatgtatatctatggattacaaacaaacacaattgtgtttaattggttgcaaaccacctataggggaacactggggcaaaggatccccatgtaNcaatgttgcagtaaNtccaggtgattgtccaccattagagttaataaacacagttattcaggatggtgatatggttgatacNggctttggtgctatggactttactacattacaggctaacaaaagtgaagttccactggatatttgtacNtctatttgcaaatatccagattatattaaaatggtgtcagaaccatatggcgacagcttatttttttatttacgaagggaacaaatgtttgttagacatttatttaatagggctggtNctgttggtgaaaatgtaccagacgatttatacattaaaggctctgggtctactgcaaatttagccagttcaaattattttcctacacctagtggttctatggttacctctgatgcccaaatattNaataaaccttattggttacaacgagcacagggccacaataatggcatttgttggggtaaccaactatttgttactgtNgtNgatactacacgcagtacaaatatgtcattatgtgctgccatatctacttcagaaNctacatataaaaaatactaactttaaNgagtacctacgacatggggaggaatatgatttacagtttatttttcaactgtgcaaaataaccttaactgcagacgttatgacatacatacattctatgaattccactattttggaggactggaattttggtNtacaacctccNccaggaggcacactagaagatacttataggtttgtaacatcccaggcaattgcttgtcaaaaacatacacctccagcacctaaagaagatgatccccttaaaaaataNactttttgggaagtaaatttaaaNgaaaagttttctgcagacctagatcagtttcctttaggacgcaaatttttactacaagcaggattNaaggccaaaccaaaatttacattaggaaaacgaaaagctacacccaccacctcatctacctctacaactgctaaacgcaaaaaacgtaagctgtaa
2
PRIMER_PRODUCT_SIZE_RANGE=200-1200
6
PRIMER_NUM_NS_ACCEPTED=0
8
PRIMER_MISPRIMING_LIBRARY=mispriming.fasta
9
PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0
14
PRIMER_LEFT_EXPLAIN=considered 4449, too many Ns 373, GC content failed 127, low tm 2109, high tm 1284, high any compl 6, high end compl 27, high repeat similarity 31, long poly-x seq 61, ok 431
15
PRIMER_RIGHT_EXPLAIN=considered 4370, too many Ns 400, GC content failed 120, low tm 2106, high tm 1190, high any compl 2, high end compl 6, high repeat similarity 6, long poly-x seq 58, ok 482
16
PRIMER_PAIR_EXPLAIN=considered 343, unacceptable product size 320, ok 23
17
PRIMER_PAIR_PENALTY=0.1407
18
PRIMER_LEFT_PENALTY=0.081493
19
PRIMER_RIGHT_PENALTY=0.059210
20
PRIMER_LEFT_SEQUENCE=tgtgctgccatatctacttcaga
21
PRIMER_RIGHT_SEQUENCE=taggtgctggaggtgtatgtttt
25
PRIMER_RIGHT_TM=59.941
26
PRIMER_LEFT_GC_PERCENT=43.478
27
PRIMER_RIGHT_GC_PERCENT=43.478
28
PRIMER_LEFT_SELF_ANY=4.00
29
PRIMER_RIGHT_SELF_ANY=2.00
30
PRIMER_LEFT_SELF_END=3.00
31
PRIMER_RIGHT_SELF_END=0.00
32
PRIMER_LEFT_MISPRIMING_SCORE=11.00, J04353 Human papillomavirus type 31 (HPV-31) complete genome.
33
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, U45909 Human papillomavirus type 45, isolate IS252, major capsid protein L1 (L1) gene, partial cds.
34
PRIMER_PAIR_MISPRIMING_SCORE=20.00, reverse EF177179 Human papillomavirus type 56 clone Qv22608, complete genome.
35
PRIMER_LEFT_END_STABILITY=6.7000
36
PRIMER_RIGHT_END_STABILITY=7.0000
37
PRIMER_PAIR_COMPL_ANY=5.00
38
PRIMER_PAIR_COMPL_END=1.00
39
PRIMER_PRODUCT_SIZE=278
40
PRIMER_PAIR_PENALTY_1=0.1407
41
PRIMER_LEFT_1_PENALTY=0.081493
42
PRIMER_RIGHT_1_PENALTY=0.059210
43
PRIMER_LEFT_1_SEQUENCE=tgtgctgccatatctacttcaga
44
PRIMER_RIGHT_1_SEQUENCE=ttaggtgctggaggtgtatgttt
46
PRIMER_RIGHT_1=1394,23
47
PRIMER_LEFT_1_TM=59.919
48
PRIMER_RIGHT_1_TM=59.941
49
PRIMER_LEFT_1_GC_PERCENT=43.478
50
PRIMER_RIGHT_1_GC_PERCENT=43.478
51
PRIMER_LEFT_1_SELF_ANY=4.00
52
PRIMER_RIGHT_1_SELF_ANY=2.00
53
PRIMER_LEFT_1_SELF_END=3.00
54
PRIMER_RIGHT_1_SELF_END=0.00
55
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, J04353 Human papillomavirus type 31 (HPV-31) complete genome.
56
PRIMER_RIGHT_1_MISPRIMING_SCORE=11.00, EF202156 Human papillomavirus type 45 isolate Qv20214, complete genome.
57
PRIMER_PAIR_1_MISPRIMING_SCORE=20.00, EF202156 Human papillomavirus type 45 isolate Qv20214, complete genome.
58
PRIMER_LEFT_1_END_STABILITY=6.7000
59
PRIMER_RIGHT_1_END_STABILITY=7.0000
60
PRIMER_PAIR_1_COMPL_ANY=5.00
61
PRIMER_PAIR_1_COMPL_END=1.00
62
PRIMER_PRODUCT_SIZE_1=279
63
PRIMER_PAIR_PENALTY_2=0.1407
64
PRIMER_LEFT_2_PENALTY=0.081493
65
PRIMER_RIGHT_2_PENALTY=0.059210
66
PRIMER_LEFT_2_SEQUENCE=tgtgctgccatatctacttcaga
67
PRIMER_RIGHT_2_SEQUENCE=tttaggtgctggaggtgtatgtt
69
PRIMER_RIGHT_2=1395,23
70
PRIMER_LEFT_2_TM=59.919
71
PRIMER_RIGHT_2_TM=59.941
72
PRIMER_LEFT_2_GC_PERCENT=43.478
73
PRIMER_RIGHT_2_GC_PERCENT=43.478
74
PRIMER_LEFT_2_SELF_ANY=4.00
75
PRIMER_RIGHT_2_SELF_ANY=2.00
76
PRIMER_LEFT_2_SELF_END=3.00
77
PRIMER_RIGHT_2_SELF_END=0.00
78
PRIMER_LEFT_2_MISPRIMING_SCORE=11.00, J04353 Human papillomavirus type 31 (HPV-31) complete genome.
79
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, U45909 Human papillomavirus type 45, isolate IS252, major capsid protein L1 (L1) gene, partial cds.
80
PRIMER_PAIR_2_MISPRIMING_SCORE=20.00, J04353 Human papillomavirus type 31 (HPV-31) complete genome.
81
PRIMER_LEFT_2_END_STABILITY=6.7000
82
PRIMER_RIGHT_2_END_STABILITY=6.6000
83
PRIMER_PAIR_2_COMPL_ANY=5.00
84
PRIMER_PAIR_2_COMPL_END=2.00
85
PRIMER_PRODUCT_SIZE_2=280
86
PRIMER_PAIR_PENALTY_3=0.2276
87
PRIMER_LEFT_3_PENALTY=0.081493
88
PRIMER_RIGHT_3_PENALTY=0.146140
89
PRIMER_LEFT_3_SEQUENCE=tgtgctgccatatctacttcaga
90
PRIMER_RIGHT_3_SEQUENCE=aaggggatcatcttctttaggtg
92
PRIMER_RIGHT_3=1410,23
93
PRIMER_LEFT_3_TM=59.919
94
PRIMER_RIGHT_3_TM=59.854
95
PRIMER_LEFT_3_GC_PERCENT=43.478
96
PRIMER_RIGHT_3_GC_PERCENT=43.478
97
PRIMER_LEFT_3_SELF_ANY=4.00
98
PRIMER_RIGHT_3_SELF_ANY=7.00
99
PRIMER_LEFT_3_SELF_END=3.00
100
PRIMER_RIGHT_3_SELF_END=1.00
101
PRIMER_LEFT_3_MISPRIMING_SCORE=11.00, J04353 Human papillomavirus type 31 (HPV-31) complete genome.
102
PRIMER_RIGHT_3_MISPRIMING_SCORE=11.00, X74481 Human papillomavirus type 52 genomic DNA.
103
PRIMER_PAIR_3_MISPRIMING_SCORE=20.00, DQ057317 Human papillomavirus type 52 isolate HK1151 L1 protein (L1) gene, partial cds.
104
PRIMER_LEFT_3_END_STABILITY=6.7000
105
PRIMER_RIGHT_3_END_STABILITY=7.9000
106
PRIMER_PAIR_3_COMPL_ANY=4.00
107
PRIMER_PAIR_3_COMPL_END=2.00
108
PRIMER_PRODUCT_SIZE_3=295
109
PRIMER_PAIR_PENALTY_4=0.2322
110
PRIMER_LEFT_4_PENALTY=0.173020
111
PRIMER_RIGHT_4_PENALTY=0.059210
112
PRIMER_LEFT_4_SEQUENCE=actgcaaatttagccagttcaaa
113
PRIMER_RIGHT_4_SEQUENCE=tttaggtgctggaggtgtatgtt
115
PRIMER_RIGHT_4=1395,23
116
PRIMER_LEFT_4_TM=60.173
117
PRIMER_RIGHT_4_TM=59.941
118
PRIMER_LEFT_4_GC_PERCENT=34.783
119
PRIMER_RIGHT_4_GC_PERCENT=43.478
120
PRIMER_LEFT_4_SELF_ANY=8.00
121
PRIMER_RIGHT_4_SELF_ANY=2.00
122
PRIMER_LEFT_4_SELF_END=2.00
123
PRIMER_RIGHT_4_SELF_END=0.00
124
PRIMER_LEFT_4_MISPRIMING_SCORE=10.00, J04353 Human papillomavirus type 31 (HPV-31) complete genome.
125
PRIMER_RIGHT_4_MISPRIMING_SCORE=11.00, U45909 Human papillomavirus type 45, isolate IS252, major capsid protein L1 (L1) gene, partial cds.
126
PRIMER_PAIR_4_MISPRIMING_SCORE=19.00, J04353 Human papillomavirus type 31 (HPV-31) complete genome.
127
PRIMER_LEFT_4_END_STABILITY=7.3000
128
PRIMER_RIGHT_4_END_STABILITY=6.6000
129
PRIMER_PAIR_4_COMPL_ANY=4.00
130
PRIMER_PAIR_4_COMPL_END=2.00
131
PRIMER_PRODUCT_SIZE_4=466
132
PRIMER_PAIR_PENALTY_5=0.2322
133
PRIMER_LEFT_5_PENALTY=0.173020
134
PRIMER_RIGHT_5_PENALTY=0.059210
135
PRIMER_LEFT_5_SEQUENCE=actgcaaatttagccagttcaaa
136
PRIMER_RIGHT_5_SEQUENCE=taggtgctggaggtgtatgtttt
138
PRIMER_RIGHT_5=1393,23
139
PRIMER_LEFT_5_TM=60.173
140
PRIMER_RIGHT_5_TM=59.941
141
PRIMER_LEFT_5_GC_PERCENT=34.783
142
PRIMER_RIGHT_5_GC_PERCENT=43.478
143
PRIMER_LEFT_5_SELF_ANY=8.00
144
PRIMER_RIGHT_5_SELF_ANY=2.00
145
PRIMER_LEFT_5_SELF_END=2.00
146
PRIMER_RIGHT_5_SELF_END=0.00
147
PRIMER_LEFT_5_MISPRIMING_SCORE=10.00, J04353 Human papillomavirus type 31 (HPV-31) complete genome.
148
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, U45909 Human papillomavirus type 45, isolate IS252, major capsid protein L1 (L1) gene, partial cds.
149
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, reverse PPHT39 Human papillomavirus ORFs.
150
PRIMER_LEFT_5_END_STABILITY=7.3000
151
PRIMER_RIGHT_5_END_STABILITY=7.0000
152
PRIMER_PAIR_5_COMPL_ANY=4.00
153
PRIMER_PAIR_5_COMPL_END=3.00
154
PRIMER_PRODUCT_SIZE_5=464
155
PRIMER_PAIR_PENALTY_6=0.2322
156
PRIMER_LEFT_6_PENALTY=0.173020
157
PRIMER_RIGHT_6_PENALTY=0.059210
158
PRIMER_LEFT_6_SEQUENCE=actgcaaatttagccagttcaaa
159
PRIMER_RIGHT_6_SEQUENCE=ttaggtgctggaggtgtatgttt
161
PRIMER_RIGHT_6=1394,23
162
PRIMER_LEFT_6_TM=60.173
163
PRIMER_RIGHT_6_TM=59.941
164
PRIMER_LEFT_6_GC_PERCENT=34.783
165
PRIMER_RIGHT_6_GC_PERCENT=43.478
166
PRIMER_LEFT_6_SELF_ANY=8.00
167
PRIMER_RIGHT_6_SELF_ANY=2.00
168
PRIMER_LEFT_6_SELF_END=2.00
169
PRIMER_RIGHT_6_SELF_END=0.00
170
PRIMER_LEFT_6_MISPRIMING_SCORE=10.00, J04353 Human papillomavirus type 31 (HPV-31) complete genome.
171
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, EF202156 Human papillomavirus type 45 isolate Qv20214, complete genome.
172
PRIMER_PAIR_6_MISPRIMING_SCORE=20.00, reverse PPHT39 Human papillomavirus ORFs.
173
PRIMER_LEFT_6_END_STABILITY=7.3000
174
PRIMER_RIGHT_6_END_STABILITY=7.0000
175
PRIMER_PAIR_6_COMPL_ANY=4.00
176
PRIMER_PAIR_6_COMPL_END=3.00
177
PRIMER_PRODUCT_SIZE_6=465
178
PRIMER_PAIR_PENALTY_7=0.2545
179
PRIMER_LEFT_7_PENALTY=0.173020
180
PRIMER_RIGHT_7_PENALTY=0.081493
181
PRIMER_LEFT_7_SEQUENCE=actgcaaatttagccagttcaaa
182
PRIMER_RIGHT_7_SEQUENCE=tctgaagtagatatggcagcaca
184
PRIMER_RIGHT_7=1138,23
185
PRIMER_LEFT_7_TM=60.173
186
PRIMER_RIGHT_7_TM=59.919
187
PRIMER_LEFT_7_GC_PERCENT=34.783
188
PRIMER_RIGHT_7_GC_PERCENT=43.478
189
PRIMER_LEFT_7_SELF_ANY=8.00
190
PRIMER_RIGHT_7_SELF_ANY=4.00
191
PRIMER_LEFT_7_SELF_END=2.00
192
PRIMER_RIGHT_7_SELF_END=3.00
193
PRIMER_LEFT_7_MISPRIMING_SCORE=10.00, J04353 Human papillomavirus type 31 (HPV-31) complete genome.
194
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, reverse FJ385268 Human papillomavirus type 58 isolate HPV58sc78, complete genome.
195
PRIMER_PAIR_7_MISPRIMING_SCORE=19.00, J04353 Human papillomavirus type 31 (HPV-31) complete genome.
196
PRIMER_LEFT_7_END_STABILITY=7.3000
197
PRIMER_RIGHT_7_END_STABILITY=8.2000
198
PRIMER_PAIR_7_COMPL_ANY=5.00
199
PRIMER_PAIR_7_COMPL_END=2.00
200
PRIMER_PRODUCT_SIZE_7=209
201
PRIMER_PAIR_PENALTY_8=0.3192
202
PRIMER_LEFT_8_PENALTY=0.173020
203
PRIMER_RIGHT_8_PENALTY=0.146140
204
PRIMER_LEFT_8_SEQUENCE=actgcaaatttagccagttcaaa
205
PRIMER_RIGHT_8_SEQUENCE=aaggggatcatcttctttaggtg
207
PRIMER_RIGHT_8=1410,23
208
PRIMER_LEFT_8_TM=60.173
209
PRIMER_RIGHT_8_TM=59.854
210
PRIMER_LEFT_8_GC_PERCENT=34.783
211
PRIMER_RIGHT_8_GC_PERCENT=43.478
212
PRIMER_LEFT_8_SELF_ANY=8.00
213
PRIMER_RIGHT_8_SELF_ANY=7.00
214
PRIMER_LEFT_8_SELF_END=2.00
215
PRIMER_RIGHT_8_SELF_END=1.00
216
PRIMER_LEFT_8_MISPRIMING_SCORE=10.00, J04353 Human papillomavirus type 31 (HPV-31) complete genome.
217
PRIMER_RIGHT_8_MISPRIMING_SCORE=11.00, X74481 Human papillomavirus type 52 genomic DNA.
218
PRIMER_PAIR_8_MISPRIMING_SCORE=19.00, X74481 Human papillomavirus type 52 genomic DNA.
219
PRIMER_LEFT_8_END_STABILITY=7.3000
220
PRIMER_RIGHT_8_END_STABILITY=7.9000
221
PRIMER_PAIR_8_COMPL_ANY=3.00
222
PRIMER_PAIR_8_COMPL_END=2.00
223
PRIMER_PRODUCT_SIZE_8=481
224
PRIMER_PAIR_PENALTY_9=0.3690
225
PRIMER_LEFT_9_PENALTY=0.309788
226
PRIMER_RIGHT_9_PENALTY=0.059210
227
PRIMER_LEFT_9_SEQUENCE=atgctagtgcttatgcagcaaat
228
PRIMER_RIGHT_9_SEQUENCE=taggtgctggaggtgtatgtttt
230
PRIMER_RIGHT_9=1393,23
231
PRIMER_LEFT_9_TM=60.310
232
PRIMER_RIGHT_9_TM=59.941
233
PRIMER_LEFT_9_GC_PERCENT=39.130
234
PRIMER_RIGHT_9_GC_PERCENT=43.478
235
PRIMER_LEFT_9_SELF_ANY=6.00
236
PRIMER_RIGHT_9_SELF_ANY=2.00
237
PRIMER_LEFT_9_SELF_END=3.00
238
PRIMER_RIGHT_9_SELF_END=0.00
239
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, EU918766 Human papillomavirus type 33 isolate LZcc12-33, complete genome.
240
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, U45909 Human papillomavirus type 45, isolate IS252, major capsid protein L1 (L1) gene, partial cds.
241
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, reverse PPHT39 Human papillomavirus ORFs.
242
PRIMER_LEFT_9_END_STABILITY=7.2000
243
PRIMER_RIGHT_9_END_STABILITY=7.0000
244
PRIMER_PAIR_9_COMPL_ANY=5.00
245
PRIMER_PAIR_9_COMPL_END=2.00
246
PRIMER_PRODUCT_SIZE_9=919