1
<!DOCTYPE HTML PUBLIC "-//IETF//DTD HTML//EN">
4
<title>Out-of-frame notation</title>
7
<BODY bgcolor="FFFFFF" link="0000FF" vlink="ff0000" text="000000" >
9
<H1><CENTER>Out-Of-Frame BLAST notation</CENTER></H1>
11
When protein aligned to the nucleotide there are 6 possibilities of
12
match at any point. In OOF alignment - upper sequence is DNAP - 3-frame
13
translated DNA. Lower sequence is protein. At any position next protein
14
base may be aligned to 6 possible bases in DNAP:
16
(TBO - traditional blast output)
18
0: 3 nucleotides missing - gap (TBO notation "-")
21
OOF alignment with DNAP:
23
DTRGGDTPQKSVFSRAQNTLWGERGDTQKRGGAQRGDIFSLWGG-GVLCV
24
| | | | | | | | | | | | | | | | |
25
D G T K F A T G G Q G Q D S G K V V
34
1: 2 nucleotides missing - "frameshift -2" (TBO notation "\\")
37
OOF alignment with DNAP:
39
DTRGGDTPQKSVFSRAQNTLWGERGDTQKRGGAQRGDIFSLWGGGGVLCV
40
| | | | | | | | | | | | | | |/ | |
41
D G T K F A T G G Q G Q D S GK V V
50
2: 1 nucletide missing - "frameshift -1" (TBO notation "\")
53
OOF alignment with DNAP:
55
DTRGGDTPQKSVFSRAQNTLWGERGDTQKRGGAQRGDIFSLWGGERGV
56
| | | | | | | | | | | | | | / | |
57
D G T K F A T G G Q G Q D S G K V
65
3: Complete match
68
OOF alignment with DNAP:
70
DTRGGDTPQKSVFSRAQNTLWGERGDTQKRGGAQRGDIFSLWGGEKRGV
71
| | | | | | | | | | | | | | | | |
72
D G T K F A T G G Q G Q D S G K V
81
4: 1 nucleotide insertion - "frameshift +1" (TBO notation "/")
84
OOF alignment with DNAP:
86
DTRGGDTPQKSVFSRAQNTLWGERGDTQKRGGAQRGDIFSLWGGVEKRGV
87
| | | | | | | | | | | | | | | \
88
D G T K F A T G G Q G Q D S G K V
97
5: 2 nucleotides insertion - "frameshift +2" (TBP notation "//")
100
OOF alignment with DNAP:
102
DTRGGDTPQKSVFSRAQNTLWGERGDTQKRGGAQRGDIFSLFLWGGEKRGV
103
| | | | | | | | | | | | | | \ | |
104
D G T K F A T G G Q G Q D S G K V
115
<address><a href="mailto:shavirin@ncbi.nlm.nih.gov">Sergei Shavirin</a></address>
116
<!-- Created: Tue Oct 17 10:07:24 EDT 2000 -->
118
Last modified: Tue Oct 17 10:56:56 EDT 2000