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# -*-Perl-*- Test Harness script for Bioperl
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# $Id: LiveSeq.t 15112 2008-12-08 18:12:38Z sendu $
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test_begin(-tests => 48,
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-requires_module => 'IO::String');
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use_ok('Bio::LiveSeq::IO::BioPerl');
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my $loader=Bio::LiveSeq::IO::BioPerl->load(-db=>"EMBL",
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-file=>test_input_file('factor7.embl'));
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my $gene=$loader->gene2liveseq(-gene_name => "factor7");
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ok ref($gene), "Bio::LiveSeq::Gene";
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is $gene->name, "factor7";
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is $gene->get_DNA->alphabet, "dna";
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is $gene->get_DNA->display_id, "HSCFVII";
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is $gene->get_DNA->accession_number, "J02933";
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is $gene, $gene->get_DNA->gene;
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is $gene->get_DNA->desc, "Human blood coagulation factor VII gene, complete cds.";
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is $gene->get_DNA->source, "Homo sapiens";
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is $gene->get_DNA->start, 1;
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is $gene->get_DNA->end, 12850;
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is $gene->maxtranscript->start, 487;
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is $gene->maxtranscript->end, 12686;
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is $gene->upbound, 487;
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is $gene->downbound, 12686;
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ok not(defined($gene->get_Repeat_Units));
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my @exons = @{$gene->get_Exons};
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my @introns = @{$gene->get_Introns};
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is scalar(@introns), 8;
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is $introns[4]->desc, "Intron D";
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is $introns[4]->start, 6592;
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is $introns[4]->end, 8306;
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is $exons[1]->desc, "optional";
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is $exons[4]->end, 6591;
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my $transcript = $gene->get_Transcripts->[0];
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my $translation = $gene->get_Translations->[0];
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is $transcript , $translation->get_Transcript;
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is $translation , $transcript->get_Translation;
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@exons = $transcript->all_Exons;
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is $exons[4]->end , 6591;
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is $exons[4]->length , 114;
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is $transcript->upstream_seq, "tcaacaggcaggggcagcactgcagagatttcatc";
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is substr($transcript->downstream_seq,0,16), "cccagcagccctggcc";
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is $transcript->position($transcript->label(666)), 666;
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is $transcript->position($transcript->label(666),9419), 95;
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is $transcript->labelsubseq(8447,undef,9419), "gt";
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is $transcript->labelsubseq(8447,2), "gt";
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is $gene->get_DNA->labelsubseq(8447,2), "gg";
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is substr($gene->get_DNA->labelsubseq(8447,undef,9419),0,16), "ggtgaccaggcttcat";
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is $gene->get_DNA, $transcript->{seq};
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my ($nothing,$whichexon) = $transcript->in_which_Exon(9419);
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is $transcript->frame(9419) , 1;
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is $transcript->frame(9420) , 2;
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is substr($translation->seq,0,16), "MVSQALRLLCLLLGLQ";
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is substr($transcript->seq,0,32), "atggtctcccaggccctcaggctcctctgcct";
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ok $transcript->translation_table(2);
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is $transcript->translation_table , 2;
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is substr($translation->seq,0,16), "MVSQAL*"; # mitochondrial table creates stop codon
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is $gene->verbose(2), 2;
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ok $gene->delete_Obj(); # to free all memory, deleting circular references