1
Number raw input seqs 4
3
Length outside bounds of 200 and 1000 0
4
Num ambiguous bases exceeds limit of 6 0
6
Mean qual score below minimum of 25 0
7
Max homopolymer run exceeds limit of 6 0
8
Num mismatches in primer exceeds limit of 0: 0
10
Number of sequences with identifiable barcode but without identifiable reverse primer: 1
12
-z truncate_only option enabled; sequences without a discernible reverse primer as well as sequences with a valid barcode not found in the mapping file may still be written.
14
Sequence length details for all sequences passing quality filters:
15
Raw len min/max/avg 244.0/276.0/255.5
16
Wrote len min/max/avg 178.0/243.0/197.0
18
Barcodes corrected/not 0/0
19
Uncorrected barcodes will not be written to the output fasta file.
20
Corrected barcodes will be written with the appropriate barcode category.
21
Corrected but unassigned sequences will not be written unless --retain_unassigned_reads is enabled.
23
Total valid barcodes that are not in mapping file 0
24
Sequences associated with valid barcodes that are not in the mapping file will not be written.
26
Barcodes in mapping file
28
Sample ct min/max/mean: 1 / 2 / 1.33
29
Sample Sequence Count Barcode
40
Total number seqs written 4
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